Biochimie最新文献_第9页

Discovery and characterization of the α-amylases cDNAs from Enchytraeus albidus shed light on the evolution of “Enchytraeus-Eisenia type” Amy homologs in Annelida 白尾 Enchytraeus α 淀粉酶 cDNA 的发现和特征描述揭示了无脊椎动物中 "Enchytraeus-Eisenia 型 "淀粉酶同源物的进化过程。

IF 3.9 3区生物学

Biochimie Pub Date : 2024-01-17 DOI: 10.1016/j.biochi.2024.01.008

Łukasz Gajda, Agata Daszkowska-Golec, Piotr Świątek

{"title":"Discovery and characterization of the α-amylases cDNAs from Enchytraeus albidus shed light on the evolution of “Enchytraeus-Eisenia type” Amy homologs in Annelida","authors":"Łukasz Gajda, Agata Daszkowska-Golec, Piotr Świątek","doi":"10.1016/j.biochi.2024.01.008","DOIUrl":"10.1016/j.biochi.2024.01.008","url":null,"abstract":"<div>Although enchytraeids have gained popularity in scientific research, fundamental questions regarding their feeding ecology and biology remain largely unexplored. This study investigates α-amylases, major digestive enzymes responsible for hydrolyzing starch and similar polysaccharides into sugars, in Enchytraeus albidus. Genetic data related to α-amylases is currently lacking for the family Enchytraeidae but also for the entire Annelida. To detect and identify coding sequences of the expressed α-amylase genes in COI-monohaplotype culture (PL-A strain) of E. albidus, we used classical “gene fishing” and transcriptomic approaches. We also compared coding sequence variants of α-amylase retrieved from transcriptomic data related to freeze-tolerant strains. Our results reveal that E. albidus possesses two distinct α-amylase genes (Amy I and Amy II) that are homologs to earthworm Eisenia fetida Ef-Amy genes. Different strains of E. albidus possess distinctive alleles of α-amylases with unique SNP patterns specific to a particular strain. Unlike Amy II, Amy I seems to be a highly polymorphic and multicopy gene. The domain architecture of the putative Amy proteins was found the same as for classical animal α-amylases with ABC-domains. A characteristic feature of Amy II is the lack of GHGA motif in the flexible loop region, similarly to many insect amylases. We identified “Enchytraeus-Eisenia type” α-amylase homologs in other clitellates and polychaetes, indicating the ancestral origin of Amy I/II proteins in Annelida. This study provides the first insight into the endogenous non-proteolytic digestive enzyme genes in potworms, discusses the evolution of Amy α-amylases in Annelida, and explores phylogenetic implications.</div>","PeriodicalId":251,"journal":{"name":"Biochimie","volume":null,"pages":null},"PeriodicalIF":3.9,"publicationDate":"2024-01-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139502049","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Dynamic modulation of transthylakoid electric potential by chloroplast ATP synthases 叶绿体 ATP 合成酶对跨叶绿体电动势的动态调节

IF 3.9 3区生物学

Biochimie Pub Date : 2024-01-13 DOI: 10.1016/j.biochi.2024.01.007

Hui Lyu, Yong-Song Zuo

{"title":"Dynamic modulation of transthylakoid electric potential by chloroplast ATP synthases","authors":"Hui Lyu, Yong-Song Zuo","doi":"10.1016/j.biochi.2024.01.007","DOIUrl":"10.1016/j.biochi.2024.01.007","url":null,"abstract":"<div>The light-induced transthylakoid membrane potential (ΔΨm) can function as a driving force to help catalyzing the formation of ATP molecules, proving a tight connection between ΔΨm and the ATP synthase. Naturally, a question can be raised on the effects of altered functioning of ATP synthases on regulating ΔΨm, which is attractive in the area of photosynthetic research. Lots of findings, when making efforts of solving this difficulty, can offer an in-depth understanding into the mechanism behind. However, the functional network on modulating ΔΨm is highly interdependent. It is difficult to comprehend the consequences of altered activity of ATP synthases on adjusting ΔΨm because parameters that have influences on ΔΨm would themselves be affected by ΔΨm. In this work, a computer model was applied to check the kinetic changes in polarization/depolarization across the thylakoid membrane (TM) regulated by the modified action of ATP synthases. The computing data revealed that under the extreme condition by numerically “switching off” the action of the ATP synthase, the complete inactivation of ATP synthase would markedly impede proton translocation at the cytb6f complex. Concurrently, the KEA3 (CLCe) porter, actively pumping protons into the stroma, further contributes to achieving a sustained low level of ΔΨm. Besides, the quantitative consequences on every particular component of ΔΨm adjusted by the modified functioning of ATP synthases were also explored. By employing the model, we bring evidence from the theoretical perspective that the ATP synthase is a key factor in forming a transmembrane proton loop thereby maintaining a propriate steady-state ΔΨm to meet variable environmental conditions.</div>","PeriodicalId":251,"journal":{"name":"Biochimie","volume":null,"pages":null},"PeriodicalIF":3.9,"publicationDate":"2024-01-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139462817","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cell-penetrating peptide and cationic liposomes mediated siRNA delivery to arrest growth of chronic myeloid leukemia cells in vitro 细胞穿透肽和阳离子脂质体介导的 siRNA 体外递送可阻止慢性髓性白血病细胞生长

IF 3.9 3区生物学

Biochimie Pub Date : 2024-01-10 DOI: 10.1016/j.biochi.2024.01.006

Vera Vysochinskaya , Yana Zabrodskaya , Olesya Dovbysh , Anton Emelyanov , Vladimir Klimenko , Nikolay Knyazev , Ivan Terterov , Marya Egorova , Alexey Bogdanov , Michael Maslov , Andrey Vasin , Michael Dubina

{"title":"Cell-penetrating peptide and cationic liposomes mediated siRNA delivery to arrest growth of chronic myeloid leukemia cells in vitro","authors":"Vera Vysochinskaya , Yana Zabrodskaya , Olesya Dovbysh , Anton Emelyanov , Vladimir Klimenko , Nikolay Knyazev , Ivan Terterov , Marya Egorova , Alexey Bogdanov , Michael Maslov , Andrey Vasin , Michael Dubina","doi":"10.1016/j.biochi.2024.01.006","DOIUrl":"10.1016/j.biochi.2024.01.006","url":null,"abstract":"<div>Gene silencing through RNA interference (RNAi) is a promising therapeutic approach for a wide range of disorders, including cancer. Non-viral gene therapy, using specific siRNAs against BCR-ABL1, can be a supportive or alternative measure to traditional chronic myeloid leukemia (CML) tyrosine kinase inhibitor (TKIs) therapies, given the prevalence of clinical TKI resistance. The main challenge for such approaches remains the development of the effective delivery system for siRNA tailored to the specific disease model.The purpose of this study was to examine and compare the efficiency of endosomolytic cell penetrating peptide (CPP) EB1 and PEG2000-decorated cationic liposomes composed of polycationic lipid 1,26-bis(cholest-5-en-3-yloxycarbonylamino)-7,11,16,20-tetraazahexacosane tetrahydrochloride (2Х3) and helper lipid 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine (DOPE) for anti-bcr-abl siRNA delivery into the K562 human CML cell line. We show that both EB1 and 2Х3-DOPE-DSPE-PEG2000 (0.62 % mol.) liposomes effectively deliver siRNA into K562 cells by endocytic mechanisms, and the use of liposomes leads to more effective inhibition of expression of the targeted gene (BCR-ABL1) and cancer cell proliferation. Taken together, these findings suggest that PEG-decorated cationic liposomes mediated siRNA delivery allows an effective antisense suppression of certain oncogenes, and represents a promising new class of therapies for CML.</div>","PeriodicalId":251,"journal":{"name":"Biochimie","volume":null,"pages":null},"PeriodicalIF":3.9,"publicationDate":"2024-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139412347","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Sucrose phosphorylase from Alteromonas mediterranea: Structural insight into the regioselective α-glucosylation of (+)-catechin 来自地中海单胞菌的蔗糖磷酸化酶：对(+)-儿茶素的区域选择性α-葡萄糖基化的结构洞察

IF 3.9 3区生物学

Biochimie Pub Date : 2024-01-09 DOI: 10.1016/j.biochi.2024.01.004

Marine Goux , Marie Demonceaux , Johann Hendrickx , Claude Solleux , Emilie Lormeau , Folmer Fredslund , David Tezé , Bernard Offmann , Corinne André-Miral

{"title":"Sucrose phosphorylase from Alteromonas mediterranea: Structural insight into the regioselective α-glucosylation of (+)-catechin","authors":"Marine Goux , Marie Demonceaux , Johann Hendrickx , Claude Solleux , Emilie Lormeau , Folmer Fredslund , David Tezé , Bernard Offmann , Corinne André-Miral","doi":"10.1016/j.biochi.2024.01.004","DOIUrl":"10.1016/j.biochi.2024.01.004","url":null,"abstract":"<div>Sucrose phosphorylases, through transglycosylation reactions, are interesting enzymes that can transfer regioselectively glucose from sucrose, the donor substrate, onto acceptors like flavonoids to form glycoconjugates and hence modulate their solubility and bioactivity. Here, we report for the first time the structure of sucrose phosphorylase from the marine bacteria Alteromonas mediterranea (AmSP) and its enzymatic properties. Kinetics of sucrose hydrolysis and transglucosylation capacities on (+)-catechin were investigated. Wild-type enzyme (AmSP-WT) displayed high hydrolytic activity on sucrose and was devoid of transglucosylation activity on (+)-catechin. Two variants, AmSP-Q353F and AmSP-P140D catalysed the regiospecific transglucosylation of (+)-catechin: 89 % of a novel compound (+)-catechin-4′-O-α-d-glucopyranoside (CAT-4′) for AmSP-P140D and 92 % of (+)-catechin-3′-O-α-d-glucopyranoside (CAT-3′) for AmSP-Q353F. The compound CAT-4′ was fully characterized by NMR and mass spectrometry. An explanation for this difference in regiospecificity was provided at atomic level by molecular docking simulations: AmSP-P140D was found to preferentially bind (+)-catechin in a mode that favours glucosylation on its hydroxyl group in position 4′ while the binding mode in AmSP-Q353F favoured glucosylation on its hydroxyl group in position 3’.</div>","PeriodicalId":251,"journal":{"name":"Biochimie","volume":null,"pages":null},"PeriodicalIF":3.9,"publicationDate":"2024-01-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S030090842400004X/pdfft?md5=42e43335b2df48c3f434f3f13832e47b&pid=1-s2.0-S030090842400004X-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139412343","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The determinants of metabolic discrepancies in aerobic glycolysis: Providing potential targets for breast cancer treatment 有氧糖酵解代谢差异的决定因素：为乳腺癌治疗提供潜在靶点

IF 3.9 3区生物学

Biochimie Pub Date : 2024-01-04 DOI: 10.1016/j.biochi.2024.01.003

Ajeesh Babu Littleflower, Sulfath Thottungal Parambil, Gisha Rose Antony, Lakshmi Subhadradevi

{"title":"The determinants of metabolic discrepancies in aerobic glycolysis: Providing potential targets for breast cancer treatment","authors":"Ajeesh Babu Littleflower, Sulfath Thottungal Parambil, Gisha Rose Antony, Lakshmi Subhadradevi","doi":"10.1016/j.biochi.2024.01.003","DOIUrl":"10.1016/j.biochi.2024.01.003","url":null,"abstract":"<div>Altered aerobic glycolysis is the robust mechanism to support cancer cell survival and proliferation beyond the maintenance of cellular energy metabolism. Several investigators portrayed the important role of deregulated glycolysis in different cancers, including breast cancer. Breast cancer is the most ubiquitous form of cancer and the primary cause of cancer death in women worldwide. Breast cancer with increased glycolytic flux is hampered to eradicate with current therapies and can result in tumor recurrence. In spite of the low order efficiency of ATP production, cancer cells are highly addicted to glycolysis. The glycolytic dependency of cancer cells provides potential therapeutic strategies to preferentially kill cancer cells by inhibiting glycolysis using antiglycolytic agents. The present review emphasizes the most recent research on the implication of glycolytic enzymes, including glucose transporters (GLUTs), hexokinase (HK), phosphofructokinase (PFK), pyruvate kinase (PK), lactate dehydrogenase-A (LDHA), associated signalling pathways and transcription factors, as well as the antiglycolytic agents that target key glycolytic enzymes in breast cancer. The potential activity of glycolytic inhibitors impinges cancer prevalence and cellular resistance to conventional drugs even under worse physiological conditions such as hypoxia. As a single agent or in combination with other chemotherapeutic drugs, it provides the feasibility of new therapeutic modalities against a wide spectrum of human cancers.</div>","PeriodicalId":251,"journal":{"name":"Biochimie","volume":null,"pages":null},"PeriodicalIF":3.9,"publicationDate":"2024-01-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139096584","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Recent advances in nano/microfluidics-based cell isolation techniques for cancer diagnosis and treatments 基于纳米/微流体的细胞分离技术在癌症诊断和治疗方面的最新进展

IF 3.9 3区生物学

Biochimie Pub Date : 2024-01-03 DOI: 10.1016/j.biochi.2024.01.001

Nahid Shanehband, Seyed Morteza Naghib

{"title":"Recent advances in nano/microfluidics-based cell isolation techniques for cancer diagnosis and treatments","authors":"Nahid Shanehband, Seyed Morteza Naghib","doi":"10.1016/j.biochi.2024.01.001","DOIUrl":"10.1016/j.biochi.2024.01.001","url":null,"abstract":"<div>Miniaturization has improved significantly in the recent decade, which has enabled the development of numerous microfluidic systems. Microfluidic technologies have shown great potential for separating desired cells from heterogeneous samples, as they offer benefits such as low sample consumption, easy operation, and high separation accuracy. Microfluidic cell separation approaches can be classified into physical (label-free) and biological (labeled) methods based on their working principles. Each method has remarkable and feasible benefits for the purposes of cancer detection and therapy, as well as the challenges that we have discussed in this article. In this review, we present the recent advances in microfluidic cell sorting techniques that incorporate both physical and biological aspects, with an emphasis on the methods by which the cells are separated. We first introduce and discuss the biological cell sorting techniques, followed by the physical cell sorting techniques. Additionally, we explore the role of microfluidics in drug screening, drug delivery, and lab-on-chip (LOC) therapy. In addition, we discuss the challenges and future prospects of integrated microfluidics for cell sorting.</div>","PeriodicalId":251,"journal":{"name":"Biochimie","volume":null,"pages":null},"PeriodicalIF":3.9,"publicationDate":"2024-01-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139093539","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Exploring NAD+ metabolism and NNAT: Insights from structure, function, and computational modeling 探索 NAD+ 代谢和 NNAT：从结构、功能和计算模型中获得启示

IF 3.9 3区生物学

Biochimie Pub Date : 2024-01-03 DOI: 10.1016/j.biochi.2024.01.002

Olamide Jeje , Sarah Otun , Chinyere Aloke , Ikechukwu Achilonu

{"title":"Exploring NAD+ metabolism and NNAT: Insights from structure, function, and computational modeling","authors":"Olamide Jeje , Sarah Otun , Chinyere Aloke , Ikechukwu Achilonu","doi":"10.1016/j.biochi.2024.01.002","DOIUrl":"10.1016/j.biochi.2024.01.002","url":null,"abstract":"<div>Nicotinamide Adenine Dinucleotide (NAD+), a coenzyme, is ubiquitously distributed and serves crucial functions in diverse biological processes, encompassing redox reactions, energy metabolism, and cellular signalling. This review article explores the intricate realm of NAD + metabolism, with a particular emphasis on the complex relationship between its structure, function, and the pivotal enzyme, Nicotinate Nucleotide Adenylyltransferase (NNAT), also known as nicotinate mononucleotide adenylyltransferase (NaMNAT), in the process of its biosynthesis. Our findings indicate that NAD + biosynthesis in humans and bacteria occurs via the same de novo synthesis route and the pyridine ring salvage pathway. Maintaining NAD homeostasis in bacteria is imperative, as most bacterial species cannot get NAD+ from their surroundings. However, due to lower sequence identity and structurally distant relationship of bacteria, including E. faecium and K. pneumonia, to its human counterpart, inhibiting NNAT, an indispensable enzyme implicated in NAD + biosynthesis, is a viable alternative in curtailing infections orchestrated by E. faecium and K. pneumonia. By merging empirical and computational discoveries and connecting the intricate NAD + metabolism network with NNAT's crucial role, it becomes clear that the synergistic effect of these insights may lead to a more profound understanding of the coenzyme's function and its potential applications in the fields of therapeutics and biotechnology.</div>","PeriodicalId":251,"journal":{"name":"Biochimie","volume":null,"pages":null},"PeriodicalIF":3.9,"publicationDate":"2024-01-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139093588","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The complex repertoire of Tityus spp. venoms: Advances on their composition and pharmacological potential of their toxins Tityus属毒液的复杂种类：关于其毒素成分和药理潜力的研究进展

IF 3.9 3区生物学

Biochimie Pub Date : 2024-01-03 DOI: 10.1016/j.biochi.2023.12.012

Gisele A. Wiezel , Isadora S. Oliveira , Mouzarllem B. Reis , Isabela G. Ferreira , Kalynka R. Cordeiro , Karla C.F. Bordon , Eliane C. Arantes

{"title":"The complex repertoire of Tityus spp. venoms: Advances on their composition and pharmacological potential of their toxins","authors":"Gisele A. Wiezel , Isadora S. Oliveira , Mouzarllem B. Reis , Isabela G. Ferreira , Kalynka R. Cordeiro , Karla C.F. Bordon , Eliane C. Arantes","doi":"10.1016/j.biochi.2023.12.012","DOIUrl":"10.1016/j.biochi.2023.12.012","url":null,"abstract":"<div>Animal venoms are a rich and complex source of components, including peptides (such as neurotoxins, anionic peptides and hypotensins), lipids, proteins (such as proteases, hyaluronidases and phospholipases) and inorganic compounds, which affect all biological systems of the envenoming victim. Their action may result in a wide range of clinical manifestations, including tachy/bradycardia, hyper/hypotension, disorders in blood coagulation, pain, edema, inflammation, fever, muscle paralysis, coma and even death. Scorpions are one of the most studied venomous animals in the world and interesting bioactive molecules have been isolated and identified from their venoms over the years. Tityus spp. are among the scorpions with high number of accidents reported in the Americas, especially in Brazil. Their venoms have demonstrated interesting results in the search for novel agents with antimicrobial, anti-viral, anti-parasitic, hypotensive, immunomodulation, anti-insect, antitumor and/or antinociceptive activities. Furthermore, other recent activities still under investigation include drug delivery action, design of anti-epileptic drugs, investigation of sodium channel function, treatment of erectile disfunction and priapism, improvement of scorpion antivenom and chelating molecules activity. In this scenario, this paper focuses on reviewing advances on Tityus venom components mainly through the modern omics technologies as well as addressing potential therapeutic agents from their venoms and highlighting this abundant source of pharmacologically active molecules with biotechnological application.</div>","PeriodicalId":251,"journal":{"name":"Biochimie","volume":null,"pages":null},"PeriodicalIF":3.9,"publicationDate":"2024-01-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139093591","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Unlocking the enigma of phenotypic drug tolerance: Mechanisms and emerging therapeutic strategies 揭开表型耐药性之谜：机制与新兴治疗策略

IF 3.9 3区生物学

Biochimie Pub Date : 2023-12-31 DOI: 10.1016/j.biochi.2023.12.009

Alok K. Mishra , Ritesh P. Thakare , Bela G. Santani , Shivraj M. Yabaji , Shivendra K. Dixit , Kishore K. Srivastava

{"title":"Unlocking the enigma of phenotypic drug tolerance: Mechanisms and emerging therapeutic strategies","authors":"Alok K. Mishra , Ritesh P. Thakare , Bela G. Santani , Shivraj M. Yabaji , Shivendra K. Dixit , Kishore K. Srivastava","doi":"10.1016/j.biochi.2023.12.009","DOIUrl":"10.1016/j.biochi.2023.12.009","url":null,"abstract":"<div>In the ongoing battle against antimicrobial resistance, phenotypic drug tolerance poses a formidable challenge. This adaptive ability of microorganisms to withstand drug pressure without genetic alterations further complicating global healthcare challenges. Microbial populations employ an array of persistence mechanisms, including dormancy, biofilm formation, adaptation to intracellular environments, and the adoption of L-forms, to develop drug tolerance. Moreover, molecular mechanisms like toxin-antitoxin modules, oxidative stress responses, energy metabolism, and (p)ppGpp signaling contribute to this phenomenon. Understanding these persistence mechanisms is crucial for predicting drug efficacy, developing strategies for chronic bacterial infections, and exploring innovative therapies for refractory infections. In this comprehensive review, we dissect the intricacies of drug tolerance and persister formation, explore their role in acquired drug resistance, and highlight emerging therapeutic approaches to combat phenotypic drug tolerance. Furthermore, we outline the future landscape of interventions for persistent bacterial infections.</div>","PeriodicalId":251,"journal":{"name":"Biochimie","volume":null,"pages":null},"PeriodicalIF":3.9,"publicationDate":"2023-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0300908423003334/pdfft?md5=eaba07b42d6835fc3491a59325726f16&pid=1-s2.0-S0300908423003334-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139069200","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparison of the calcium signaling alterations in GABA-ergic medium spiny neurons produced from iPSCs of different origins 比较不同来源 iPSCs 产生的 GABA 能中棘神经元的钙信号改变

IF 3.9 3区生物学

Biochimie Pub Date : 2023-12-30 DOI: 10.1016/j.biochi.2023.12.011

Arina A. Oshkolova , Dmitriy A. Grekhnev , Anna A. Kruchinina , Lilia D. Belikova , Egor A. Volovikov , Olga S. Lebedeva , Alexandra N. Bogomazova , Vladimir A. Vigont , Maria A. Lagarkova , Elena V. Kaznacheyeva

{"title":"Comparison of the calcium signaling alterations in GABA-ergic medium spiny neurons produced from iPSCs of different origins","authors":"Arina A. Oshkolova , Dmitriy A. Grekhnev , Anna A. Kruchinina , Lilia D. Belikova , Egor A. Volovikov , Olga S. Lebedeva , Alexandra N. Bogomazova , Vladimir A. Vigont , Maria A. Lagarkova , Elena V. Kaznacheyeva","doi":"10.1016/j.biochi.2023.12.011","DOIUrl":"10.1016/j.biochi.2023.12.011","url":null,"abstract":"<div>Disease models based on induced pluripotent stem cells (iPSCs) are in high demand because of their physiological adequacy and well-reproducibility of the pathological phenotype. Nowadays, the most common approach to generate iPSCs is the reprogramming of somatic cells using vectors based on lentivirus or Sendai virus. We have previously shown impairments of calcium signaling including store-operated calcium entry in Huntington's disease-specific iPSCs-based GABA-ergic medium spiny neurons. However, different approaches for iPSCs generation make it difficult to compare the models since the mechanism of reprogramming may influence the electrophysiological properties of the terminally differentiated neurons. Here, we have studied the features of calcium homeostasis in GABA-ergic medium spiny neurons differentiated from iPSCs obtained from fibroblasts of the same donor using different methods. Our data demonstrated that there were no significant differences neither in calcium influx through the store-operated channels, nor in the levels of proteins activating this type of calcium entry in neurons differentiated from iPSCs generated with lenti- and Sendai viruses-based approaches. We also found no differences in voltage-gated calcium entry for these neurons. Thus, we clearly showed that various methods of cell reprogramming result in similar deregulations in neuronal calcium signaling which substantiates the ability to combine the experimental data on functional studies of ion channels in models based on iPSCs obtained by different methods and expands the prospects for the use of biobanking.</div>","PeriodicalId":251,"journal":{"name":"Biochimie","volume":null,"pages":null},"PeriodicalIF":3.9,"publicationDate":"2023-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139068891","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0