Biochimie最新文献

{"title":"Convergence of plant sterols and host eukaryotic cell-derived defensive lipids at the infectious pathogen-host interface","authors":"Darab Ghadimi ,&nbsp;Regina Fölster-Holst ,&nbsp;Sophia Blömer ,&nbsp;Michael Ebsen ,&nbsp;Christoph Röcken ,&nbsp;Jumpei Uchiyama ,&nbsp;Shigenobu Matsuzaki ,&nbsp;Wilhelm Bockelmann","doi":"10.1016/j.biochi.2024.12.001","DOIUrl":"10.1016/j.biochi.2024.12.001","url":null,"abstract":"<div><div>Plant sterols (PSs) exhibit intrinsic functions such as antibacterial effects. Their effects simultaneously on both host-mediated and bacteria-mediated pathogenesis are not yet fully understood. We hypothesized that when absorptive cells, defensive cells and detoxer cells are cultured together, their convergent response to an infectious pathogen depends on the molecular mimicry between the ingested sterols and their own defensive lipids. A human triple cell co-culture model incorporating colonocytes, macrophages, and hepatocytes was established. Cocultures were stimulated with <em>Klebsiella pneumoniae</em> 52145 (Kp52145) in the presence of pure plant sterol (β-sitosterol, PS) for 6 h. Changes in the structural health markers of the stimulated cocultured cells and their immune response and biochemical markers of pathogenicity were determined. PS significantly inhibited the secretion of cytokines induced by Kp52145. Cell viability was higher in the Kp52145 + PS group compared to the Kp52145 alone group. PS decreased Kp52145-induced marker of pathogenicity (SOD), accompanied by reduced levels of interleukin-1β (IL-1β), interleukin-6 (IL-6), mannose binding lectin (MBL), and pentraxin 3 (PTX3) which are the mediators and enzymes associated with the inflammatory response to an infectious-inflamed milieu. PS recovered Kp52145-decreased peroxidase (POX), catalase (CAT), complement component 3 (C3), and high-density lipoprotein cholesterol (HDL-C) values. Convergence of ingested plant sterols and host eukaryotic cell-derived defensive lipids mitigates the disruptive effects of bacterial toxic effector molecules. Structural or immunological similarities (molecular mimicry) between ingested plant sterols and host defensive lipids play an important role in modulating bacterial signalling that occurs at the pathogen-host interface and in the mitigation of infection- and inflammation-driven pathological processes.</div></div>","PeriodicalId":251,"journal":{"name":"Biochimie","volume":"231 ","pages":"Pages 35-45"},"PeriodicalIF":3.3,"publicationDate":"2024-12-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142796522","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Metabolomic characterization of a new strain of microalgae by GC-MS method with the introduction of a deuterium label","authors":"Anna Vishnevskaya ,&nbsp;Anton Bashilov ,&nbsp;Sergey Osipenko ,&nbsp;Albert Kireev ,&nbsp;Maria Sinetova ,&nbsp;Eugene Nikolaev ,&nbsp;Yury Kostyukevich","doi":"10.1016/j.biochi.2024.11.015","DOIUrl":"10.1016/j.biochi.2024.11.015","url":null,"abstract":"<div><div>Microalgae are active producers of various compounds, including toxic substances. However, their metabolism is very diverse and insufficiently known. We demonstrate an approach that includes growing a new strain of cyanobacterium <em>Leptolyngbya</em> sp. (IPPAS B-1204) on an isotopically labeled medium (D₂O) and evaluating the metabolomic composition of these microorganisms after deuterium uptake. Despite the low resolution of the GC-MS method, the interpretation of the obtained spectra allowed us to find out not only the amount of the embedded isotope label but also to assume the position in the structure where the label is embedded. We present the results of reliably detecting more than 30 compounds with isotope labels belonging to various classes of biological compounds produced by this cyanobacterium, revealing the metabolic pathways of entry of this label. We also demonstrate that the synthesis of unsaturated fatty acids is suppressed under the growth on D₂O medium. In addition, we found an isotopic effect in the chromatographic separation of isotopically labeled compounds in gas chromatography. These data can be used in the future both for the identification of compounds and the analysis of the biosynthesis pathways of secondary biologically active compounds and in the analysis of the production of isotopically labeled standards of compounds.</div></div>","PeriodicalId":251,"journal":{"name":"Biochimie","volume":"231 ","pages":"Pages 23-34"},"PeriodicalIF":3.3,"publicationDate":"2024-12-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142792994","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Macromolecules from mushrooms, venoms, microorganisms, and plants for diabetes treatment - Progress or setback?","authors":"Asmaa Chbel ,&nbsp;Ayoub Lafnoune ,&nbsp;Imane Nait Irahal ,&nbsp;Noureddine Bourhim","doi":"10.1016/j.biochi.2024.07.004","DOIUrl":"10.1016/j.biochi.2024.07.004","url":null,"abstract":"<div><div>Diabetes is a substantial public health issue, while its prevalence continues to rise worldwide, affecting millions of persons between the ages of 20 and 80, the development of new therapeutic classes improving glycemic control and consequently micro and macrovascular complications are needed. Today, diabetes treatment is daily for life, and should not be interrupted. However, insulin secretagogues medications, and exogenous self-administration of insulin provide efficient antidiabetic effects, but their misuse leads to hypoglycemic complications besides other risks, hence the need to look for other natural products not to use solely but in concert with others types of medications. In this review, we will highlight briefly the pathophysiology of diabetes and its complications, then we will report the main bioactive macromolecules derived from various sources of natural products providing anti-diabetic properties. However, further researches need to be carried out to face the limitations hampering the development of effective natural drugs for diabetes treatment.</div></div>","PeriodicalId":251,"journal":{"name":"Biochimie","volume":"227 ","pages":"Pages 119-128"},"PeriodicalIF":3.3,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141602371","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Assessment of membrane labelling mechanisms with exogenous fatty acids and detergents in bacteria","authors":"Laila Zaatouf ,&nbsp;Kiran Kumar ,&nbsp;Isabelle Marcotte ,&nbsp;Dror E. Warschawski","doi":"10.1016/j.biochi.2024.05.024","DOIUrl":"10.1016/j.biochi.2024.05.024","url":null,"abstract":"<div><div>Labelling of bacterial membranes using exogenous fatty acids has proven to be a valuable tool to investigate molecular interactions by in-cell solid-state nuclear magnetic resonance (ssNMR) spectroscopy, notably with antimicrobial peptides. However, the mechanism by which this labelling takes place in non-mutated bacteria has not yet been investigated. In this work, we propose a rapid method to assess the fate of the fatty acids during the labelling of bacteria, involving two different methylation schemes and gas chromatography coupled to mass spectrometry. We applied this approach to Gram(+) and Gram(−) bacteria grown with deuterated palmitic acid under different conditions. We assessed the extent of labelling, then the resulting membrane rigidity by ²H ssNMR. Our results reveal that the labelling mechanism depends on the detergent used to micellize the fatty acids. This labelling can be either <em>active</em> or <em>passive</em>, whether the fatty acids are metabolized and used in the phospholipids biosynthesis, or remain unmodified in the membrane. We discuss the best labelling protocol for studying peptide-membrane interactions.</div></div>","PeriodicalId":251,"journal":{"name":"Biochimie","volume":"227 ","pages":"Pages 12-18"},"PeriodicalIF":3.3,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141201583","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Tailor-made vincristine-liposomes for tumor targeting","authors":"Ariana Abawi ,&nbsp;Ana-Maria Trunfio-Sfarghiu ,&nbsp;Céline Thomann ,&nbsp;Emma Petiot ,&nbsp;Giovanna Lollo ,&nbsp;Thierry Granjon ,&nbsp;Agnès Girard-Egrot ,&nbsp;Ofelia Maniti","doi":"10.1016/j.biochi.2024.07.017","DOIUrl":"10.1016/j.biochi.2024.07.017","url":null,"abstract":"<div><div>To ensure selective targeting based on membrane fluidity and physico-chemical compatibility between the biological membrane of the target cell and the lipid membrane of the liposomes carriers. Lipid-based carriers as liposomes with varying membrane fluidities were designed for delivering vincristine, an anti-tumor compound derived from Madagascar's periwinkle. Liposomes, loaded with vincristine, were tested on prostate, colon, and breast cancer cell lines alongside non-tumor controls. Results showed that vincristine-loaded liposomes with fluid membranes significantly decreased the viability of cancer cell lines compared to controls. Confocal microscopy revealed the intracellular release of vincristine, evidenced by disrupted mitosis-specific labeling of actin filaments in metastatic prostate cell lines. This highlights the crucial role of membrane fluidity in the development of lipid-based drug carriers, offering a promising and cost-effective option for targeting cancer cells as an alternative to conventional strategies.</div></div>","PeriodicalId":251,"journal":{"name":"Biochimie","volume":"227 ","pages":"Pages 35-46"},"PeriodicalIF":3.3,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141879942","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Systemic and joint adipose tissue lipids and their role in osteoarthritis","authors":"Natalia Zapata-Linares ,&nbsp;Léa Loisay ,&nbsp;Diego de Haro ,&nbsp;Francis Berenbaum ,&nbsp;Thomas Hügle ,&nbsp;Jeroen Geurts ,&nbsp;Xavier Houard","doi":"10.1016/j.biochi.2024.09.015","DOIUrl":"10.1016/j.biochi.2024.09.015","url":null,"abstract":"<div><div>Osteoarthritis (OA) is a major disease whose prevalence increases with aging, sedentary lifestyles, and obesity. The association between obesity and OA has been well documented, but the precise mechanisms underlying this heightened risk remain unclear. While obesity imposes greater forces on joints, systemic fat-derived factors such as lipids or adipokine may potentially act on the pathophysiology of OA, but the exact role of these factors in weight-bearing and non-weight-bearing joints remains elusive. Intra-articular adipose tissues (IAAT) have gained significant attention for actively participating in OA pathogenesis by interacting with various joint tissues. Lipid content has been proposed as a diagnostic target for early OA detection and a potential source of biomarkers. Moreover, targeting a specific IAAT called infrapatellar fat pad (IFP) and its lipids hold promise for attenuating OA-associated inflammation. Conversely, bone marrow adipose tissue (BMAT), which was long thought to be an inert filling tissue, is now increasingly considered a dynamic tissue whose volume and lipid content regulate bone remodeling in pathological conditions. Given OA's ability to alter adipose tissues, particularly those within the joint (IFP and BMAT), and the influence of adipose tissues on OA pathogenesis, this review examines the lipids produced by OA-associated adipose tissues, shedding light on their potential role in OA pathophysiology and highlighting them as potential therapeutic targets.</div></div>","PeriodicalId":251,"journal":{"name":"Biochimie","volume":"227 ","pages":"Pages 130-138"},"PeriodicalIF":3.3,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142334289","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Unlocking the potential of histone modification in regulating bone metabolism","authors":"Jiayuan Zhang,&nbsp;Hanghang Liu,&nbsp;Yao Liu,&nbsp;En Luo,&nbsp;Shibo Liu","doi":"10.1016/j.biochi.2024.08.004","DOIUrl":"10.1016/j.biochi.2024.08.004","url":null,"abstract":"<div><div>Bone metabolism plays a crucial role in maintaining normal bone tissue homeostasis and function. Imbalances between bone formation and resorption can lead to osteoporosis, osteoarthritis, and other bone diseases. The dynamic and complex process of bone remodeling is driven by various factors, including epigenetics. Histone modification, one of the most important and well-studied components of epigenetic regulation, has emerged as a promising area of research in bone metabolism. Different histone proteins and modification sites exert diverse effects on osteogenesis and osteoclastogenesis. In this review, we summarize recent progress in understanding histone modifications in bone metabolism, including specific modification sites and potential regulatory enzymes. Comprehensive knowledge of histone modifications in bone metabolism could reveal new therapeutic targets and treatment strategies for bone diseases.</div></div>","PeriodicalId":251,"journal":{"name":"Biochimie","volume":"227 ","pages":"Pages 286-298"},"PeriodicalIF":3.3,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142001571","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Inhibitors and activators for myotoxic phospholipase A2-like toxins from snake venoms – A structural overview","authors":"Guilherme H.M. Salvador ,&nbsp;Fábio F. Cardoso ,&nbsp;Bruno Lomonte ,&nbsp;Marcos R.M. Fontes","doi":"10.1016/j.biochi.2024.07.016","DOIUrl":"10.1016/j.biochi.2024.07.016","url":null,"abstract":"<div><div>Snakebite envenomations result in acute and chronic physical and psychological health effects on their victims, leading to a substantial socio-economic burden in tropical and subtropical countries. Local necrosis is one of the serious effects caused by envenomation, primarily induced by snake venoms from the Viperidae family through the direct action of components collectively denominated as myotoxins, including the phopholipase A₂-like (PLA₂-like) toxins. Considering the limitations of antivenoms in preventing the rapid development of local tissue damage caused by envenomation, the use of small molecule therapeutics has been suggested as potential first-aid treatments or as adjuvants to antivenom therapy. In this review, we provide an overview of the structural interactions of molecules exhibiting inhibitory activity toward PLA₂-like toxins. Additionally, we discuss the implications for the myotoxic mechanism of PLA₂-like toxins and the molecules involved in their activation, highlighting key differences between activators and inhibitors. Finally, we integrate all these results to propose a classification of inhibitors into three different classes and five sub-classes. Taking into account the structural and affinity information, we compare the different inhibitors/ligands to gain a deeper understanding of the structural basis for the effective inhibition of PLA₂-like toxins. By offering these insights, we aim to contribute to the search for new and efficient inhibitor molecules to complement and improve current therapy by conventional antivenoms.</div></div>","PeriodicalId":251,"journal":{"name":"Biochimie","volume":"227 ","pages":"Pages 231-247"},"PeriodicalIF":3.3,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141876923","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Alpha hemolysin of Escherichia coli induces a necrotic-like procoagulant state in platelets","authors":"Kenia Pérez Vázquez ,&nbsp;Julia Tau ,&nbsp;M. Florencia Leal Denis ,&nbsp;Claudio M. Fader ,&nbsp;Mariano A. Ostuni ,&nbsp;Pablo J. Schwarzbaum ,&nbsp;Vanesa Herlax","doi":"10.1016/j.biochi.2024.06.001","DOIUrl":"10.1016/j.biochi.2024.06.001","url":null,"abstract":"<div><div>Uropathogenic strains of <em>E. coli</em><span><span> (UPEC) is a leading cause of sepsis, deploying multiple </span>virulence factors<span><span><span> to evade host immune responses. Notably, alpha-hemolysin (HlyA) produced by UPEC is implicated in septic symptoms associated with </span>bacteremia, correlating with </span>thrombocytopenia, a critical indicator of organ dysfunction and a predictor of poorer patient prognosis.</span></span></div><div><span><span>This study meticulously explores the impact of sublytic concentrations of HlyA on platelets. Findings reveal that HlyA triggers an increase in intracellular calcium, activating </span>calpain<span> and exposing phosphatidylserine to the cell surface, as validated by flow cytometric experiments. </span></span>Electron microscopy<span><span> reveals a distinctive balloon-like shape in HlyA-treated platelets, indicative of a procoagulant state. The toxin induces the release of procoagulant extracellular vesicles and the secretion of alpha and </span>dense granules. Overall, the results point to HlyA inducing a necrotic-like procoagulant state in platelets.</span></div><div>The effects of sublytic concentrations of HlyA on both erythrocytes and platelets could have a potential impact on capillary microcirculation<span>. Targeting HlyA emerges as a viable therapeutic strategy to mitigate the adverse effects of UPEC infections, especially in South American countries where these infections are endemic, underscoring its significance as a potential therapeutic target.</span></div></div>","PeriodicalId":251,"journal":{"name":"Biochimie","volume":"227 ","pages":"Pages 1-14"},"PeriodicalIF":3.3,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141302233","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Stealing survival: Iron acquisition strategies of Mycobacterium tuberculosis","authors":"Gauri Shankar,&nbsp;Yusuf Akhter","doi":"10.1016/j.biochi.2024.06.006","DOIUrl":"10.1016/j.biochi.2024.06.006","url":null,"abstract":"<div><div><em>Mycobacterium tuberculosis</em> (<em>Mtb</em>), the causative agent of tuberculosis (TB), faces iron scarcity within the host due to immune defenses. This review explores the importance of iron for <em>Mtb</em> and its strategies to overcome iron restriction. We discuss how the host limits iron as an innate immune response and how <em>Mtb</em> utilizes various iron acquisition systems, particularly the siderophore-mediated pathway. The review illustrates the structure and biosynthesis of mycobactin, a key siderophore in <em>Mtb</em>, and the regulation of its production. We explore the potential of targeting siderophore biosynthesis and uptake as a novel therapeutic approach for TB. Finally, we summarize current knowledge on <em>Mtb</em>'s iron acquisition and highlight promising directions for future research to exploit this pathway for developing new TB interventions.</div></div>","PeriodicalId":251,"journal":{"name":"Biochimie","volume":"227 ","pages":"Pages 37-60"},"PeriodicalIF":3.3,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141433651","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}