Biochimie最新文献

{"title":"The cysteine protease legumain decreases glucose metabolism and enhances fatty acid uptake in human myotubes","authors":"Nimo Mukhtar Mohamud Osoble ,&nbsp;Ngoc Nguyen Lunde ,&nbsp;Abbas Jafari ,&nbsp;G. Hege Thoresen ,&nbsp;Rigmor Solberg ,&nbsp;Arild C. Rustan","doi":"10.1016/j.biochi.2024.12.012","DOIUrl":"10.1016/j.biochi.2024.12.012","url":null,"abstract":"<div><div>Skeletal muscle has an important role in whole body energy metabolism and various proteases are involved in skeletal muscle functions. We have previously identified the cysteine protease legumain in cultured human skeletal muscle cells. However, the potential role of legumain in regulation of energy metabolism remains unexplored. This study aimed to investigate cellular uptake, processing, and activation of prolegumain in human myotubes. Additionally, we sought to determine the effects of prolegumain on energy substrate metabolism in these cells. During differentiation of human myoblast to myotubes, legumain mRNA expression and activity were upregulated. Interestingly, legumain activity in myotubes was inversely correlated with the body mass index (BMI) of the obese cell donors. Myotubes exposed to conditioned medium enriched in prolegumain during the last two days of differentiation demonstrated the capacity to internalize and process prolegumain into its active form. Pre-treatment with prolegumain induced a metabolic shift towards increased fatty acid uptake in myotubes, as evidenced by elevated oleic acid uptake whereas glucose uptake and oxidation were reduced. The metabolic changes were not reversed by a legumain inhibitor, indicating a different mechanism for this effect. The metabolic alterations were accompanied by increased mRNA expression of the fatty acid transporter CD36, whereas the glucose transporter GLUT1 mRNA level remained unchanged. These findings suggest that legumain may play a regulatory role in skeletal muscle energy metabolism, highlighting its potential as a novel therapeutic target of metabolic disorders.</div></div>","PeriodicalId":251,"journal":{"name":"Biochimie","volume":"231 ","pages":"Pages 127-136"},"PeriodicalIF":3.3,"publicationDate":"2024-12-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142908045","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The neutralisation and inhibitory effects of new tRNA-mimetic aptamer against cytotoxin-induced cytotoxicity in human skin keratinocytes","authors":"Jia Jin Hiu,&nbsp;Hock Siew Tan,&nbsp;Michelle Khai Khun Yap","doi":"10.1016/j.biochi.2024.12.011","DOIUrl":"10.1016/j.biochi.2024.12.011","url":null,"abstract":"<div><div>Cytotoxin (CTX) is the major venom toxin in cobra venom, accounting for the dermonecrosis in cobra envenomed patients. Conventional antibody-based antivenoms have limited efficacy in the treatment for CTX-manifested dermonecrosis, highlighting the urgent needs for discovery of new therapeutics. This study aims to discover oligonucleotide-based aptamers which target the functional epitopes of CTX to inhibit its cytotoxicity. Genomic tRNA Database (GtRNAdb) was acquired for the computational selection and design of potential tRNA-mimetic aptamers (AptRNAs) targeting CTX. The binding characteristics of the AptRNAs were further validated by electromobility shift assay (EMSA), and enzyme-linked oligonucleotide assay (ELONA). The best candidate was also examined for neutralisation potency and efficacy. The computational analyses identified six consensus AptRNA clusters, whereby three AptRNAs showed significant binding for CTX with an ascending ranking from AptRNA4 &gt; AptRNA6 &gt; AptRNA5. These AptRNAs bind to the functional epitopes of CTX. AptRNA6 had the highest binding specificity towards CTX, with a binding affinity (K_D) of 14.45 nM. Furthermore, AptRNA6 exhibited the best neutralisation and rescue potency in CTX-induced cytotoxicity. Even at low treatment concentrations, AptRNA6 also demonstrated the highest potency in rescuing experimental post-envenomed model by cobra venoms of medically important species. Overall, the findings concluded the potential of AptRNA6 as synthetic biotherapeutic against cobra venom CTX.</div></div>","PeriodicalId":251,"journal":{"name":"Biochimie","volume":"231 ","pages":"Pages 116-126"},"PeriodicalIF":3.3,"publicationDate":"2024-12-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143592469","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Characterization of Arabidopsis thaliana FRATAXIN HOMOLOG in heme catabolism","authors":"Jing Zhang,&nbsp;Yingying Zhou,&nbsp;Qianyi Duan,&nbsp;Xinhe Xu,&nbsp;Xiao Wang,&nbsp;Jia Wang,&nbsp;Lin Liu","doi":"10.1016/j.biochi.2024.12.010","DOIUrl":"10.1016/j.biochi.2024.12.010","url":null,"abstract":"<div><div>Frataxin plays vital roles in various iron related processes. <em>Arabidopsis thaliana</em> FRATAXIN HOMOLOG (<em>At</em>FH) is the first identified plant frataxin and has been found to regulate the last step of heme biosynthesis. Here, we report the involvement of <em>At</em>FH in heme catabolism by regulating the activity of heme oxygenase. <em>At</em>FH forms a homodimer, and its crystal structure shows the dimeric interactions. A structural comparison with known frataxin structures suggests the iron binding sites, and the site for heme oxygenase activity is possibly located in a region containing Glu78. The results indicate a previously uncharacterized role of plant frataxin in heme catabolism.</div></div>","PeriodicalId":251,"journal":{"name":"Biochimie","volume":"231 ","pages":"Pages 110-115"},"PeriodicalIF":3.3,"publicationDate":"2024-12-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142900796","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluating efflux pump inhibition in Staphylococcus aureus 1199B strain using thiadiazine-derived compounds: In vitro and in silico approaches","authors":"Priscilla Ramos Freitas ,&nbsp;Ana Carolina Justino de Araújo ,&nbsp;Isaac Moura Araújo ,&nbsp;Ray Silva Almeida ,&nbsp;João Arthur de Oliveira Borges ,&nbsp;Clara Mariana Gonçalves Lima ,&nbsp;Cícera Datiane Morais Oliveira-Tintino ,&nbsp;Cícera Laura Roque Paulo ,&nbsp;Gustavo Marinho Miranda ,&nbsp;José Bezerra de Araújo-Neto ,&nbsp;José Weverton Almeida-Bezerra ,&nbsp;Igor José dos Santos Nascimento ,&nbsp;João Xavier de Araújo-Júnior ,&nbsp;Edeildo Ferreira da Silva-Júnior ,&nbsp;Thiago Mendonça de Aquino ,&nbsp;Francisco Jaime Bezerra Mendonca Junior ,&nbsp;Emmanuel Silva Marinho ,&nbsp;Hélcio Silva dos Santos ,&nbsp;Irwin Rose Alencar de Menezes ,&nbsp;Saulo Relison Tintino ,&nbsp;Henrique Douglas Melo Coutinho","doi":"10.1016/j.biochi.2024.12.009","DOIUrl":"10.1016/j.biochi.2024.12.009","url":null,"abstract":"<div><div>Thiadiazines are heterocyclic compounds known for some pharmacological activities. However, the ability of these compounds and their derivatives to act as antibacterial agents and inhibitors of the efflux system in resistant bacteria remains unknown. This study aims to evaluate the antibacterial and NorA efflux pump inhibitory activities of thiadiazine-derived compounds (IJ14, IJ15, IJ16, IJ17, IJ18, IJ19, and IJ20) against the <em>Staphylococcus aureus</em> 1199B strain. Minimum Inhibitory Concentration (MIC) tests and antibacterial activity assessment through NorA efflux system inhibition were performed using microdilution assays in 96-well plates. Additionally, ethidium bromide (EtBr) fluorescence emission assays were conducted to evaluate efflux system inhibition. The methodology revealed that the IJ17 and IJ20 compounds presented MIC values of 256 and 597.3 μg/mL, respectively. The efflux pump inhibition assessment using the microdilution method showed significant results for all compounds, which also increased the fluorescence rates emitted by EtBr. Consequently, thiadiazine-derived compounds exhibit promising results in targeting a key bacterial resistance mechanism, underscoring the need for further studies, such as molecular tests, to evaluate their mechanism of action and clarify the feasibility and efficacy of these compounds as antibacterial agents.</div></div>","PeriodicalId":251,"journal":{"name":"Biochimie","volume":"231 ","pages":"Pages 104-109"},"PeriodicalIF":3.3,"publicationDate":"2024-12-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142873762","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"50 years since the concept of homeoviscous adaptation","authors":"Dmitry A. Los,&nbsp;Anna V. Leusenko","doi":"10.1016/j.biochi.2024.12.008","DOIUrl":"10.1016/j.biochi.2024.12.008","url":null,"abstract":"<div><div>This mini review focuses on the phenomenon of homeoviscous adaptation (HVA). The concept, which dominated for decades, had a significant impact on membrane and lipid research. It includes the functional characterization of biological membranes and their domains, the role of lipids and fatty acids in cell metabolic control, and the characterization of fatty acid desaturases and their roles in membrane properties modulation. This hypothesis led to the discovery of a feed-back manner of desaturase expression and membrane-associated temperature sensors in bacteria.</div></div>","PeriodicalId":251,"journal":{"name":"Biochimie","volume":"231 ","pages":"Pages 98-103"},"PeriodicalIF":3.3,"publicationDate":"2024-12-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142873759","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Distinctive aspects of ligand binding to G4 DNA structure flanked by the double helix","authors":"Liana L. Tevonyan,&nbsp;Timerkhan M. Fatkullin,&nbsp;Artemy D. Beniaminov,&nbsp;Dmitry N. Kaluzhny","doi":"10.1016/j.biochi.2024.12.005","DOIUrl":"10.1016/j.biochi.2024.12.005","url":null,"abstract":"<div><div>Except for telomeres, G4 DNA structures in the human genome can be formed only within the context of double-stranded DNA. DNA duplexes flanking the G4 structure may potentially affect the G4 architecture and the binding of G4-specific ligands. Here, we examine the interaction of TMPyP4, NMM, and PDS ligands with three structures formed by the same DNA fragment containing the (GGGT)₄ sequence: the G4 in duplex (dsG4), G4 in single-stranded DNA (ssG4) and perfect duplex DNA (ds). To design a structure-specific fluorescent sensor, single thymine loops or proximal positions in DNA duplex were modified with FAM. Ligand-induced fluorescence quenching revealed a preferential binding of TMPyP4 and NMM with the dsG4 and ssG4 structures over the flanking duplex part or double-stranded DNA. PDS could not quench the fluorophores attached to single-nucleotide loops of the G4 DNA, although gel mobility assay confirmed tight binding of the ligand to the ssG4 or dsG4 structures. We hypothesize that the selectivity of the ligands for G4 loops compared to duplexes is responsible for the high quenching efficiency. Distinctive features of ligand interactions with G4 DNA in a duplex context suggest the potential for developing specific ligands for the genomic G4 structure.</div></div>","PeriodicalId":251,"journal":{"name":"Biochimie","volume":"230 ","pages":"Pages 166-171"},"PeriodicalIF":3.3,"publicationDate":"2024-12-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142824908","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Achatina fulica haemocyanin-derived peptides as novel antimicrobial agents","authors":"Andrés Esteban Pereira ,&nbsp;Libardo Suarez ,&nbsp;Tanya Roman ,&nbsp;Fanny Guzmán ,&nbsp;Leidy Sierra ,&nbsp;Bladimiro Rincón-Orozco ,&nbsp;William Hidalgo","doi":"10.1016/j.biochi.2024.12.007","DOIUrl":"10.1016/j.biochi.2024.12.007","url":null,"abstract":"<div><div>Haemocyanin-derived peptides were previously found in semi-purified fractions of mucus secretion from the snail <em>Achatina fulica</em>, which exhibited an inhibitory effect on <em>Staphylococcus aureus</em> strains. Here, an <em>in silico</em> rational design strategy was employed to generate new antimicrobial peptides (AMPs) from <em>A. fulica</em> haemocyanin-derived peptides (AfH). The designed peptides were chemically synthetized using the Fmoc strategy, and their antimicrobial activity against <em>Escherichia coli</em> and <em>S. aureus</em> strains was evaluated using the broth microdilution method. In addition, the cytotoxic activity on Vero, HaCat, and human erythrocyte cells was also determined. The results demonstrated that 15-residue alpha-helical and cationic synthetic peptides exhibited the highest biological activity against Gram-positive strains, with minimum inhibitory concentrations (MIC) in the range from 7.5 to 30 μM. The positive selectivity index suggests a higher selectivity, primarily on the microorganisms evaluated, but not on eukaryotic cells. In this study, <em>A. fulica</em> hemocyanin was identified as an appropriate protein model for the rational design of AMPs against bacteria of public health significance. Further studies are required to evaluate the activity of the peptides on Gram-negative bacteria other than <em>E. coli</em>.</div></div>","PeriodicalId":251,"journal":{"name":"Biochimie","volume":"231 ","pages":"Pages 84-97"},"PeriodicalIF":3.3,"publicationDate":"2024-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142840604","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Helicid: A novel Anti-Staphylococcus aureus adjuvant","authors":"Yufen Li ,&nbsp;Haofang Zhou ,&nbsp;Teri Gele ,&nbsp;Chunjie Hu ,&nbsp;Chang Liu ,&nbsp;Wu Song ,&nbsp;Lin Wei ,&nbsp;Danning Song ,&nbsp;Mengli Jin ,&nbsp;Yating Tang ,&nbsp;Qingjie Li ,&nbsp;Shuang Jiang ,&nbsp;Gang Yuan ,&nbsp;Xin Su","doi":"10.1016/j.biochi.2024.12.002","DOIUrl":"10.1016/j.biochi.2024.12.002","url":null,"abstract":"<div><div>Staphylocoagulase (Coa) plays a critical role in the pathogenicity of <em>Staphylococcus aureus</em> (<em>S. aureus</em>). The present study was undertaken to investigate the underlying mechanism which helicid (HEL) suppressed the virulence factor Coa, as well as to assess the synergistic inhibitory effects of HEL in conjunction with antibiotics, thereby establishing the potential of HEL as an antibacterial adjuvant. We employed coagulation and biofilm assays to comprehensively assess the inhibitory impact of HEL on <em>S. aureus</em> pathogenicity. The thermal shift assay demonstrated that HEL exerted a direct impact on the protein stability of Coa, evidenced by a 6 °C change in melting temperature (Δ<em>Tm</em>) at a concentration of 100 μM. HEL binding to Coa proteins was further validated by molecular dynamics simulations and fluorescence quenching. Molecular docking and point mutation assays identified S23 and D112 as crucial binding sites for HEL and Coa. Furthermore, HEL has been observed to potentiate the bactericidal properties of ceftaroline fosamil (CEF-F), concurrently diminishing the resistance exhibited by <em>S. aureus</em> towards CEF-F, as demonstrated by antibiotic synergy tests and resistance induction assays. The combination of HEL and CEF-F effectively reduced the number of bacteria and improved the survival of both <em>Galleria mellonella</em> larvae and mice. Additionally, a significant decrease was observed in the levels of TNF-α, IL-6, and IFN-γ in mice broncho-alveolar lavage fluid (BALF). Ultimately, our findings confirmed that the direct binding of HEL to Coa could diminish the pathogenicity of <em>S. aureus</em>. Moreover, the combination with CEF-F substantially reduced the lethality associated with <em>S. aureus</em>-infected pneumonia and extended the efficacy of the antibiotic.</div></div>","PeriodicalId":251,"journal":{"name":"Biochimie","volume":"231 ","pages":"Pages 46-60"},"PeriodicalIF":3.3,"publicationDate":"2024-12-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142840605","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Combination of chemotherapy and c-MET inhibitors has synergistic effects in c-MET overexpressing pancreatic cancer cells","authors":"Fatemeh Moosavi ,&nbsp;Roya Firoozi ,&nbsp;Marjan Tavakkoli ,&nbsp;Somayeh Nazari ,&nbsp;Alireza Alipour ,&nbsp;Omidreza Firuzi","doi":"10.1016/j.biochi.2024.12.006","DOIUrl":"10.1016/j.biochi.2024.12.006","url":null,"abstract":"<div><div>Pancreatic ductal adenocarcinoma (PDAC) remains as one of the most lethal malignancies. c-MET is an important oncogenic kinase involved in PDAC progression. We determined the anticancer effect of c-MET inhibitors, crizotinib and cabozantinib, combined with chemotherapeutic agents, doxorubicin, oxaliplatin and gemcitabine, against different PDAC and a lung adenocarcinoma cell line expressing different levels of c-MET. MTT assay was performed to assess cell growth inhibition. Synergistic combinations were evaluated in spheroid cultures, while apoptosis was determined through Hoechst33258 staining. The effect of drug combinations on cell cycle and apoptosis induction was examined by RNase/PI flow cytometric assay. We also evaluated reactive oxygen species (ROS) levels using 2′,7′-dichlorofluorescein-diacetate (DCFH-DA) assay to explore the possible mechanisms contributing to synergism. Combination of crizotinib or cabozantinib with doxorubicin exhibited synergistic effects in c-MET overexpressing cells. Conversely, combinations of c-MET inhibitors with other agents were additive or even antagonistic. Combination index (CI) values calculated with Calcusyn software were 0.631–0.730 for crizotinib and 0.542–0.746 for cabozantinib co-administered with doxorubicin. These synergistic combinations showed significant spheroid growth inhibition and apoptosis induction in Suit-2, c-MET dependent PDAC cells. These combinations also significantly increased the number of cells in both apoptotic sub-G1 phase and the G2/M phase compared to single-drug treatment. Increased ROS production seemed to be a possible mechanism underlying synergism. In conclusion, c-MET inhibitors synergize with DNA damaging agent, doxorubicin, in cancer cells with c-MET overexpression, indicating that these combination therapies may be a promising cancer therapeutic strategy.</div></div>","PeriodicalId":251,"journal":{"name":"Biochimie","volume":"231 ","pages":"Pages 73-83"},"PeriodicalIF":3.3,"publicationDate":"2024-12-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142831180","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Optimized high-yield expression of envelope glycoprotein domain III from dengue virus serotypes 1 to 4","authors":"Disharee Mallick ,&nbsp;Vanshika Tyagi ,&nbsp;Anjali Saroj ,&nbsp;Mandar Bhutkar ,&nbsp;Vivek Kumar ,&nbsp;Manjima Das ,&nbsp;Rishav Madhukalya ,&nbsp;Shweta Choudhary ,&nbsp;Rohit Gupta ,&nbsp;Vishakha Singh ,&nbsp;Dilip Kumar ,&nbsp;Shailly Tomar ,&nbsp;Rajesh Kumar","doi":"10.1016/j.biochi.2024.12.003","DOIUrl":"10.1016/j.biochi.2024.12.003","url":null,"abstract":"<div><div>Dengue virus (DENV) envelope glycoprotein Domain III (EDIII) is critical for viral entry, highly immunogenic, and induces robust neutralizing antibody response. It is a prominent candidate for designing subunit-based vaccines and can also be harnessed as an antigenic bait for isolation of neutralizing human mAbs. Here, we describe an optimized method for high-yield expression of recombinant domain EDIII protein from DENV serotypes 1 to 4 in different <em>Escherichia coli (E. coli)</em> expression strains. The DENV EDIII proteins show differential expression patterns in tested <em>E. coli</em> expression strains. The structural integrity of the purified and refolded proteins is further validated using the Circular Dichroism (CD) spectroscopy and Fourier Transform Infrared (FTIR) spectroscopic analysis. The functional validation of the purified refolded DENV EDIII proteins through Enzyme-linked Immunosorbent Assay (ELISA) and co-immunoprecipitation (Co-IP) exhibits efficient binding with a well-characterized humanized neutralizing mAb 513. Further, we compared the potency of purified EDIII in blocking viral through competitive inhibition assay. Our study highlights that a universal expression system may not be an ideal approach for all DENV EDIII protein expression.</div></div>","PeriodicalId":251,"journal":{"name":"Biochimie","volume":"231 ","pages":"Pages 61-72"},"PeriodicalIF":3.3,"publicationDate":"2024-12-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142822989","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}