Biochimie最新文献_第7页

IF 3.3 3区生物学

Biochimie Pub Date : 2025-02-22 DOI: 10.1016/S0300-9084(25)00029-X

引用次数: 0

Difference in the rearrangement of quail histone H1 allelic variants during divergent selection for reduction of body mass coupled to the food withdraw 鹌鹑组蛋白H1等位基因变异在减少体重的分散选择过程中的重排差异与食物缺失。

IF 3.3 3区生物学

Biochimie Pub Date : 2025-02-21 DOI: 10.1016/j.biochi.2025.02.008

Andrzej Kowalski , Sebastian Knaga

{"title":"Difference in the rearrangement of quail histone H1 allelic variants during divergent selection for reduction of body mass coupled to the food withdraw","authors":"Andrzej Kowalski , Sebastian Knaga","doi":"10.1016/j.biochi.2025.02.008","DOIUrl":"10.1016/j.biochi.2025.02.008","url":null,"abstract":"<div><div>This study was undertaken to show whether chromatin phenotype(s) related to histone H1 polymorphism(s) exhibit connection with breeding practice (selection for reduction of body mass) and/or physiological trait (withdraw of the food) of the organism. For this purpose, a genetic diversity between quail lines selected for high and low reduction of body mass after transient food withdrawal was examined using variation in the histone H1 allelic expression. Symmetric and asymmetric expression of the isoforms of histone H1.b and histone H1.d was identified in 2-D PAGE due to various molecular weight and different intensities, respectively. Low (locus H1.d) to moderate (locus H1.b) differentiated populations conform to Hardy-Weinberg equilibrium but distribution of the phenotypes significantly differ between them. Whereas frequency of the same phenotype of histone H1.b is different between selected lines, the frequency of the same phenotypes of histone H1.d is similar in both selected lines. Thus, line-specific promotion of the phenotypes of histone H1.b suggest a response to conducted selection. Unlike this, a line-nonspecific arrangement of the phenotypes of histone H1.d appear due to the stress evoked by food withdrawal ongoing during the selection. Such a result indicate that histone H1 allelic variants possess individual impact on chromatin states and/or processes associated with breeding practices and physiological conditions of the organism. According to this, functional individualization is a characteristic feature of histone H1 polymorphic variants.</div></div>","PeriodicalId":251,"journal":{"name":"Biochimie","volume":"233 ","pages":"Pages 75-80"},"PeriodicalIF":3.3,"publicationDate":"2025-02-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143485008","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Molecular mechanism of selenite reduction by Bacillus amyloliquefaciens BB61 based on transcriptome analysis 基于转录组分析的解淀粉芽孢杆菌BB61还原亚硒酸盐的分子机制

IF 3.3 3区生物学

Biochimie Pub Date : 2025-02-21 DOI: 10.1016/j.biochi.2025.02.005

Yujie Wang , Fan Wan , Huiqin Xue , Yiqiong Hang , Caixia Pei , Yang Lu

{"title":"Molecular mechanism of selenite reduction by Bacillus amyloliquefaciens BB61 based on transcriptome analysis","authors":"Yujie Wang , Fan Wan , Huiqin Xue , Yiqiong Hang , Caixia Pei , Yang Lu","doi":"10.1016/j.biochi.2025.02.005","DOIUrl":"10.1016/j.biochi.2025.02.005","url":null,"abstract":"<div><div>The microbial conversion of selenite represents an effective detoxification and assimilation process, although the underlying mechanisms remain incompletely understood. In this study, strain BB61 was a probiotic isolated from piglet feces and identified as <em>Bacillus amyloliquefaciens</em>, which could almost completely reduce 0.1 g/L Na<sub>2</sub>SeO<sub>3</sub> to SeNPs within 48h. We investigated the potential mechanisms of selenite reduction in this strain through transcriptome sequencing and qPCR. The transcriptome analysis revealed the up-regulation of 829 genes and the down-regulation of 892 genes in response to 1 g/L Se treatment (padj <0.05) in <em>Bacillus amyloliquefaciens BB61</em>. GO (Gene Ontology) enrichment analysis indicated that DEGs (Differentially expressed genes) were predominantly associated with transmembrane transporters, ion transmembrane transport, cytoplasmic and cell membrane composition, cell movement and localization, and carbon metabolism. Additionally, the KEGG (Encyclopedia of Genes and Genomes) pathway annotation analysis revealed that the DEGs were primarily involved in the pentose phosphate pathway, pyruvate metabolism, pyrimidine metabolism, cofactor biosynthesis, and other pathways (<em>P</em> < 0.05). Among the highly expressed reductases, thioredoxin reductase (TrxA/B), nitrite reductase (NfsA), and selenite reductase (NamA) were all found to be up-regulated. Consequently, this study established a reduction pathway model for Se (IV), offering new insights into the molecular mechanisms underlying the bioreduction of selenite to form SeNPs.</div></div>","PeriodicalId":251,"journal":{"name":"Biochimie","volume":"233 ","pages":"Pages 36-46"},"PeriodicalIF":3.3,"publicationDate":"2025-02-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143485013","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Identification and characterization of a laterally transferred alternative oxidase (AOX) in a terrestrial insect, the dipteran Pseudolycoriella hygida 陆生昆虫双翅目水合假科里菌（Pseudolycoriella hygida）体内横向转移替代氧化酶（AOX）的鉴定和表征。

IF 3.3 3区生物学

Biochimie Pub Date : 2025-02-21 DOI: 10.1016/j.biochi.2025.02.007

Nadia Monesi , Guilherme Magre Fernandes , Felipe Berti Valer , João Vítor Cardoso Uliana , Vitor Trinca , Ana Elisa Caleiro Seixas Azzolini , Eduardo Gorab , Luciane Carla Alberici

{"title":"Identification and characterization of a laterally transferred alternative oxidase (AOX) in a terrestrial insect, the dipteran Pseudolycoriella hygida","authors":"Nadia Monesi , Guilherme Magre Fernandes , Felipe Berti Valer , João Vítor Cardoso Uliana , Vitor Trinca , Ana Elisa Caleiro Seixas Azzolini , Eduardo Gorab , Luciane Carla Alberici","doi":"10.1016/j.biochi.2025.02.007","DOIUrl":"10.1016/j.biochi.2025.02.007","url":null,"abstract":"<div><div>Alternative oxidase (AOX) (EC 1.10.3.11) is a terminal oxidase in the mitochondrial inner membrane that branches the canonical electron transport system (ETS). AOX is ubiquitous in plants, frequently found in fungi and protists and presents a more sporadic distribution in metazoans. More recently, AOX has gained attention due to its potential application in gene therapy for treatment of mitochondrial diseases. Here we characterized the AOX in the basal Dipteran, <em>Pseudolycoriella hygida</em> using a combination of genomic analyses, molecular, functional and <em>in vivo</em> survival assays. <em>AOX</em> is a single copy gene that encodes three developmental stage specific protein isoforms. AOX localizes to the mitochondria in adult thoracic muscles, which present cyanide-resistant respiration that is sensitive to the AOX inhibitor salicylhydroxamic acid (SHAM). Both the cyanide-resistant respiration and AOX levels gradually increase during aging, but are not influenced by thermal stress. Thoracic mitochondria respire using substrates derived from several metabolic routes, such as pyruvate, proline, acylcarnitine, NADH and glycerol-3P, and present values of oxidative phosphorylation capacity ((P-L)/E = 0.70) and coupling (P/L = 4.35; L/E = 0.21). Adult flies exhibit a high survival resistance for SHAM-sensitive complex III inhibition. Together, our results demonstrate the presence of a functional AOX in a terrestrial arthropod and provide insights regarding AOX function in animals and evolution of respiratory systems in metazoans. <em>Psl. hygida</em> emerges as a natural and valuable model for comprehensive AOX research at the whole-organism level which complements models expressing the heterologous enzyme.</div></div>","PeriodicalId":251,"journal":{"name":"Biochimie","volume":"233 ","pages":"Pages 60-74"},"PeriodicalIF":3.3,"publicationDate":"2025-02-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143485011","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Nitazoxanide inhibits pili assembly by targeting BamB to synergize with polymyxin B against drug-resistant Escherichia coli Nitazoxanide通过靶向BamB与多粘菌素B协同靶向耐药大肠杆菌抑制菌毛组装。

IF 3.3 3区生物学

Biochimie Pub Date : 2025-02-19 DOI: 10.1016/j.biochi.2025.02.006

Wenwen Li , Bingjie Ji , Boyu Li, Minghui Du, Linwei Wang, Jiale Tuo, Hongmei Zhou, Jian Gong, Yongshan Zhao

{"title":"Nitazoxanide inhibits pili assembly by targeting BamB to synergize with polymyxin B against drug-resistant Escherichia coli","authors":"Wenwen Li , Bingjie Ji , Boyu Li, Minghui Du, Linwei Wang, Jiale Tuo, Hongmei Zhou, Jian Gong, Yongshan Zhao","doi":"10.1016/j.biochi.2025.02.006","DOIUrl":"10.1016/j.biochi.2025.02.006","url":null,"abstract":"<div><div>Gram-negative bacteria rely on pili assembly for pathogenicity, with the chaperone-usher (CU) pathway regulating pilus biogenesis. Nitazoxanide (NTZ) inhibits CU pathway-mediated P pilus biogenesis by specifically interfering with the proper folding of the outer membrane protein (OMP) usher, primarily mediated by the β-barrel assembly machinery (BAM) complex. In this study, we identified the BAM complex components BamB and the BamA POTRA2 domain as key binding targets for NTZ. Molecular dynamics simulations and Bio-Layer Interferometry revealed that BamB residues S61 and R195 are critical for NTZ binding. NTZ activated the Cpx two-component system and induced inner membrane perturbations, which resulted from the accumulation of misfolded P pilus subunits. Upregulation of the <em>ibpAB</em> gene, which protects the bacteria against NTZ-induced oxidative stress, was also observed. Importantly, NTZ combined with polymyxin B enhanced the latter's antibacterial activity against both susceptible and MCR-positive <em>E. coli</em> strains. This enhancement was achieved through NTZ-induced increases in inner membrane permeability, oxidative stress, and inhibition of efflux pump activity and biofilm formation. This study provides new insights into the antimicrobial mechanism of NTZ and highlights its potential as an antibiotic adjuvant by targeting BamB to inhibit the CU pathway, restoring the efficacy of polymyxin B against multidrug-resistant bacteria.</div></div>","PeriodicalId":251,"journal":{"name":"Biochimie","volume":"233 ","pages":"Pages 47-59"},"PeriodicalIF":3.3,"publicationDate":"2025-02-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143473204","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Crystal structure of Leishmania orientalis triosephosphate isomerase at 1.88 Å resolution and its specific inhibitors 1.88 Å分辨率下东方利什曼原虫三磷酸异构酶的晶体结构及其特异性抑制剂。

IF 3.3 3区生物学

Biochimie Pub Date : 2025-02-19 DOI: 10.1016/j.biochi.2025.02.004

Buabarn Kuaprasert , Ubolsree Leartsakulpanich , Pinpunya Riangrungroj , Wichai Pornthanakasem , Wipa Suginta , Mathirut Mungthin , Saovanee Leelayoova , Duangnapa Kiriwan , Kiattawee Choowongkomon

{"title":"Crystal structure of Leishmania orientalis triosephosphate isomerase at 1.88 Å resolution and its specific inhibitors","authors":"Buabarn Kuaprasert , Ubolsree Leartsakulpanich , Pinpunya Riangrungroj , Wichai Pornthanakasem , Wipa Suginta , Mathirut Mungthin , Saovanee Leelayoova , Duangnapa Kiriwan , Kiattawee Choowongkomon","doi":"10.1016/j.biochi.2025.02.004","DOIUrl":"10.1016/j.biochi.2025.02.004","url":null,"abstract":"<div><div><em>Leishmania orientalis</em>, previously called <em>L. siamensis</em>, is a new species characterized as causing cutaneous leishmaniasis in Thailand. This study solves the crystal structure of the <em>L. orientalis</em> triosephosphate isomerase (<em>Lo</em>TIM) in apo form at 1.88 Å resolution by using molecular replacement method. Tyrosine118 presents in the LoTIM protein sequence, whereas <em>L. mexicana</em> and <em>Trypanosoma cruzi</em> TIMs have a relative Cys118, which plays a major role in their specific ligand binding. Sulfur atom of the Cys57 thiol group is covalently bound to an arsenic (As) atom present in the precipitating solution. Although the electron density of loop-6 (Gly174-Tyr175-Gly176-Lys177-Val178) is missing in the structure due to this region lacking rigidity, the biological assembly of the two monomers of the <em>Lo</em>TIM crystal structures are like that of <em>L. mexicana</em> and <em>T. cruzi</em>. 3D molecular protein-ligand docking was performed using the dimeric interfacial pocket of the enzyme as a ligand-binding receptor to identify its specific inhibitors. Five potential inhibiting compounds, including NSC639174, NSC606498, NSC110039, NSC58446, and NSC345647, were obtained with their <em>IC</em><sub>50</sub> 2.79 ± 0.10, 3.28 ± 0.80, 3.67 ± 0.11, 4.59 ± 0.87 and 15.44 ± 0.14 μM, respectively. However, specific inhibition assays against TIMs from <em>L. orientalis</em> and rabbit muscle indicate that NSC639174 and NSC110039 are the most potent inhibitors for <em>Lo</em>TIM, whereas NSC58446 inhibits well both the parasitic and rabbit enzymes.</div></div>","PeriodicalId":251,"journal":{"name":"Biochimie","volume":"233 ","pages":"Pages 27-35"},"PeriodicalIF":3.3,"publicationDate":"2025-02-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143473266","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The prolyl oligopeptidase and α-synuclein connection revisited 重新审视脯氨酸寡肽酶和α-突触核蛋白的联系。

IF 3.3 3区生物学

Biochimie Pub Date : 2025-02-19 DOI: 10.1016/j.biochi.2025.02.003

Roos Van Elzen , Yannick Waumans , Sangeeta Nath , Pieter Van der Veken , Sonja Kerkhoff , Evert Van Dijk , Markus Morawski , Steffen Roßner , Yves Engelborghs , Ingrid De Meester , Anne-Marie Lambeir

{"title":"The prolyl oligopeptidase and α-synuclein connection revisited","authors":"Roos Van Elzen , Yannick Waumans , Sangeeta Nath , Pieter Van der Veken , Sonja Kerkhoff , Evert Van Dijk , Markus Morawski , Steffen Roßner , Yves Engelborghs , Ingrid De Meester , Anne-Marie Lambeir","doi":"10.1016/j.biochi.2025.02.003","DOIUrl":"10.1016/j.biochi.2025.02.003","url":null,"abstract":"<div><div>The aim of this work was to revisit the connection between prolyl oligopeptidase (PREP) and α-synuclein (aSyn) by presenting novel data from cell free and cellular assays and to discuss the results in a contemporary context.</div><div>The aSyn aggregation process was studied using fluorescence correlation spectroscopy and thioflavin-T fluorescence. Binding sites for PREP on the aSyn sequence were determined using peptide arrays. Subcellular localisation of PREP and stress markers were studied using double staining immunofluorescence microscopy in SH-SY5Y cells with and without overexpression of aSyn and PREP, before and after differentiation, and with or without proteolytic stress induced by proteasome inhibition.</div><div>The interaction between PREP and aSyn was found to be weak and transient. It promotes the early phases of aggregation but does not affect the rate of β-fibril formation. Moreover, this interaction is not dependent upon the C-terminal prolines of aSyn, but is affected by PREP inhibitors and interferes with PREP substrate binding. Although present in the same cellular compartments, there is little evidence for a strong physical association of PREP with aggresomes and stress markers. Instead, there is colocalization with aSyn in the cell periphery and neurites.</div><div>There is evidence for a binding site for peptides much longer than the usual PREP substrates. The modular assembly of molecular machines and the observation that PREP's protein-protein interactions are tuneable by active site inhibitors, lead to the hypothesis that this binding site features in the cross-talk between autophagy and neuron-specific pathways involving vesicle transport and protein secretion.</div></div>","PeriodicalId":251,"journal":{"name":"Biochimie","volume":"233 ","pages":"Pages 1-13"},"PeriodicalIF":3.3,"publicationDate":"2025-02-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143473206","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Editor's Note to '27-hydroxycholesterol decreases cell proliferation in colon cancer cell lines' [Biochimie 153 (2018) 171-180] 编者按。

IF 3.3 3区生物学

Biochimie Pub Date : 2025-02-06 DOI: 10.1016/j.biochi.2025.02.001

引用次数: 0

Fluor NMR study of amino acid derived ligand to study TSPO 氨基酸衍生配体的荧光核磁共振研究TSPO。

IF 3.3 3区生物学

Biochimie Pub Date : 2025-02-04 DOI: 10.1016/j.biochi.2025.01.015

Luminita Duma , Severine Schneider , Agathe Martinez , Cathy Hachet , Frederic Bihel , Jean-Jacques Lacapere

{"title":"Fluor NMR study of amino acid derived ligand to study TSPO","authors":"Luminita Duma , Severine Schneider , Agathe Martinez , Cathy Hachet , Frederic Bihel , Jean-Jacques Lacapere","doi":"10.1016/j.biochi.2025.01.015","DOIUrl":"10.1016/j.biochi.2025.01.015","url":null,"abstract":"<div><div>Translocator protein (TSPO, 18 kDa), previously known as peripheral-type benzodiazepine receptor, is an evolutionarily conserved membrane protein involved in various physiological processes and patho-physiological conditions. The endogeneous TSPO ligand is a polypeptide of 9 kDa, but dipeptides with biological activity have been previously synthesized and characterized. Herein, we synthesized a phenyl alanine derived ligand with a <sup>19</sup>F labelling which opens prospective for <sup>19</sup>F-MRI and potential <sup>18</sup>F-PET applications. We characterized the coexistence of two conformers that are not equally sensitive to the media used for membrane protein studies. Interaction studies with the recombinant mouse TSPO (mTSPO) in different membrane-mimicking environments are presented using <sup>19</sup>F NMR enabling structure/function characterizations. A change in the mTSPO environment from pure detergent to lipid/detergent mixture reveals different exchange rates between bound and free ligand forms. Competition experiments with the high-affinity drug ligand (<em>R</em>)-PK 11195 suggests that phenyl alanine derived ligand binds in the same protein cavity.</div></div>","PeriodicalId":251,"journal":{"name":"Biochimie","volume":"233 ","pages":"Pages 14-26"},"PeriodicalIF":3.3,"publicationDate":"2025-02-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143367059","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Metabolic adaptation mechanisms of glycogen reduction and lipid accumulation in testicular protection in Daurian ground squirrels during hibernation 达斡尔地松鼠冬眠期间睾丸保护中糖原减少和脂质积累的代谢适应机制。

IF 3.3 3区生物学

Biochimie Pub Date : 2025-02-03 DOI: 10.1016/j.biochi.2025.01.014

Ming-Di Wang , Lu-Fan Li , Yu-Jing Yan , Xing-Chen Wang , Le Chen , Kai Dang , Zhe Wang , Hui-Ping Wang

{"title":"Metabolic adaptation mechanisms of glycogen reduction and lipid accumulation in testicular protection in Daurian ground squirrels during hibernation","authors":"Ming-Di Wang , Lu-Fan Li , Yu-Jing Yan , Xing-Chen Wang , Le Chen , Kai Dang , Zhe Wang , Hui-Ping Wang","doi":"10.1016/j.biochi.2025.01.014","DOIUrl":"10.1016/j.biochi.2025.01.014","url":null,"abstract":"<div><div>The role of glycogen and lipid metabolism in the testes of Daurian ground squirrels (<em>Spermophilus dauricus</em>) during different stages of the hibernation cycle and their influence on reproductive function remain poorly understood. This study examined testicular morphology across hibernation stages and investigated potential molecular mechanisms. Results showed that: (1) Spermatocyte density was reduced in the torpor group compared to the pre-hibernation (PRE) group, suggesting a suppression of spermatogenesis during torpor. In the post-hibernation (POST) group, reduced spermatocyte density was speculated to correspond to the initial phase of spermatocyte maturation into spermatozoa. (2) Glycogen content was lower during interbout arousal (IBA), while glycogen phosphorylase (GP) activity was significantly elevated compared to the other stages. Sertoli cell density was higher in the IBA group relative to the torpor group, suggesting that elevated GP activity facilitates glycogen breakdown, providing glycolytic substrates for Sertoli cells during this phase. (3) During torpor, triglyceride and fatty acid levels, along with fatty acid synthase and acetyl-CoA carboxylase activities, remained consistent with PRE levels. These findings suggest that fatty acids are crucial for maintaining testicular reproductive function during torpor. In contrast, lipid metabolism indicators declined during the near post-hibernation (NP) and POST stages, likely supporting the rapid reactivation of reproductive processes required for the upcoming breeding season. In summary, this study highlights a dynamic interplay between lipid and glycogen metabolism across hibernation stages, with the transition from lipid-based metabolism during torpor to glycogen utilization during IBA playing a pivotal role in sustaining testicular homeostasis in Daurian ground squirrels.</div></div>","PeriodicalId":251,"journal":{"name":"Biochimie","volume":"232 ","pages":"Pages 133-141"},"PeriodicalIF":3.3,"publicationDate":"2025-02-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143256160","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0