Zhongguo yao li xue bao = Acta pharmacologica Sinica最新文献_第7页

Scavenging effect of chinonin on free radicals studied with quantum chemistry. 用量子化学方法研究了甲壳素对自由基的清除作用。

Zhongguo yao li xue bao = Acta pharmacologica Sinica Pub Date : 1999-06-01

H Y Zhang

引用次数: 0

Protective effect of melatonin on injuried cerebral neurons is associated with bcl-2 protein over-expression. 褪黑素对脑损伤神经元的保护作用与bcl-2蛋白过表达有关。

Zhongguo yao li xue bao = Acta pharmacologica Sinica Pub Date : 1999-05-01

X Ling, L M Zhang, S D Lu, X J Li, F Y Sun

引用次数: 0

Circumvention of tumor multidrug resistance by a new annonaceous acetogenin: atemoyacin-B. 一种新的无毒醋酸原atemoyacin-B规避肿瘤多药耐药的研究。

Zhongguo yao li xue bao = Acta pharmacologica Sinica Pub Date : 1999-05-01

L W Fu, Q C Pan, Y J Liang, H B Huang

{"title":"Circumvention of tumor multidrug resistance by a new annonaceous acetogenin: atemoyacin-B.","authors":"L W Fu, Q C Pan, Y J Liang, H B Huang","doi":"","DOIUrl":"","url":null,"abstract":"Aim: To explore the effect of atemoyacin-B (Ate) on overcoming multidrug resistance (MDR).Methods: Bullatacin (Bul) was used as a positive control. Cytotoxic effects of Bul and Ate were studied with cell culture of human MDR breast adenocarcinoma cells, MCF-7/Dox and human KBv200 cells, and their parental sensitive cell lines MCF-7 and KB. Cytotoxicity was determined by tetrazolium (MTT) assay. The function of P-glycoprotein (P-gp) was examined by Fura 2-AM assay. Cellular accumulation of doxorubicin (Dox) was determined by fluorescence spectrophotometry. Apoptosis was measured by flow cytometry.Results: IC50 of Ate for MCF-7/Dox, MCF-7, KBv200, and KB cells were 122, 120, 1.34, and 1.27 nmol.L-1, respectively. IC50 of Bul for MCF-7/Dox, MCF-7, KBv200, and KB cells were 0.60, 0.59, 0.04, and 0.04 nmol.L-1, respectively. The cytotoxicities of Bul and Ate to MDR cells were similar to those to parental sensitive cells. Bul and Ate markedly increased cellular Fura-2 and Dox accumulation in MCF-7/Dox cells, but not in MCF-7 cells. The rates of apoptosis in MDR cells were similar to those in sensitive cells induced by Ate.Conclusion: There was no cross-resistance of P-gp positive MCF-7/Dox and KBv200 cell lines to Bul and Ate as compared with their sensitive P-gp negative MCF-7 and KB cell lines. The mechanism of the circumvention of MDR was associated with the decrease of P-gp function and the increase of cellular drug accumulation in MDR cells.","PeriodicalId":24002,"journal":{"name":"Zhongguo yao li xue bao = Acta pharmacologica Sinica","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1999-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21532719","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Dynamic digital fluorescence ratio imaging of cell calcium in vascular endothelial cells. 血管内皮细胞钙的动态数字荧光比成像。

Zhongguo yao li xue bao = Acta pharmacologica Sinica Pub Date : 1999-05-01

C Y Kwan, T K Kwan

{"title":"Dynamic digital fluorescence ratio imaging of cell calcium in vascular endothelial cells.","authors":"C Y Kwan, T K Kwan","doi":"","DOIUrl":"","url":null,"abstract":"Aim: To study the spatial and temporal distribution of intracellular Ca2+ concentration in cultured bovine pulmonary artery endothelial (BPAE) cells.Methods: Cultured BPAE cells were loaded with Fura-2 and observed under an inverted microscope coupled to a microfluorimeter, which enables pixel-to-pixel ratio imaging of the BPAE cells in real time.Results: Addition of Ca2+ 1-2 mmol.L-1 to BPAE cells, which were exposed to Ca(2+)-free medium containing egtazic acid, resulted in a transient elevation of cytosolic Ca2+ concentration, which rapidly returned to the resting level. Biphasic elevation (a larger transient phase followed by a smaller sustained phase) of intracellular Ca2+ concentration was observed upon the addition of ATP (via activation of surface membrane receptor). 4-Chloro-3-ethyl phenol (CEP; an activator of Ca(2+)-induced Ca2+ channels) potently induced elevation of Ca2+ level. Cyclopiazonic acid (CPA; an inhibitor of endoplasmic reticulum Ca(2+)-ATPase pump) offered a more sustained elevation of Ca2+. In most cases, the highest level of Ca2+ elevation was observed around the cell peripheries, sometimes at rest and particularly upon stimulation. Ca2+ elevation associated with nuclear complex seemed to be higher compared to that in the cytosolic compartment.Conclusion: Changes of cell Ca2+ upon stimulation by various agents that acted at different intracellular sites were found to be temporarily and spatially heterogenous among BPAE cells. At the single cell level, Ca2+ elevation seemed to occur initially near the peripheral region followed by the nuclear region. This study raised the possibility that nuclear Ca2+ and cytosolic Ca2+ might be regulated independently in BPAE cells.","PeriodicalId":24002,"journal":{"name":"Zhongguo yao li xue bao = Acta pharmacologica Sinica","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1999-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21531601","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Antagonistic effects of Ginkgo biloba extract on adhesion of monocytes and neutrophils to cultured cerebral microvascular endothelial cells. 银杏叶提取物对培养的脑微血管内皮细胞粘附单核细胞和中性粒细胞的拮抗作用。

Zhongguo yao li xue bao = Acta pharmacologica Sinica Pub Date : 1999-05-01

J P Xu, Y C Rui, T J Li

引用次数: 0

Apoptosis and necrosis induced by sulfur mustard in Hela cells. 硫芥诱导Hela细胞凋亡和坏死。

Zhongguo yao li xue bao = Acta pharmacologica Sinica Pub Date : 1999-05-01

J Sun, Y X Wang, M J Sun

引用次数: 0

6,7-dimethoxycoumarin attenuated cisplatin-induced DNA interstrand crosslink and DNA-protein crosslink in primary cultured rabbit kidney proximal tubular cells. 6,7-二甲氧基香豆素减弱顺铂诱导的兔肾近端小管细胞DNA链间交联和DNA-蛋白交联。

Zhongguo yao li xue bao = Acta pharmacologica Sinica Pub Date : 1999-05-01

S J Liu, S W Zhou

引用次数: 0

Quercetin decreased heart rate and cardiomyocyte Ca2+ oscillation frequency in rats and prevented cardiac hypertrophy in mice. 槲皮素降低大鼠心率和心肌细胞Ca2+振荡频率，防止小鼠心肌肥厚。

Zhongguo yao li xue bao = Acta pharmacologica Sinica Pub Date : 1999-05-01

Y Wang, H Y Wang, Z K Yuan, X N Zhao, J X Wang, Z X Zhang

{"title":"Quercetin decreased heart rate and cardiomyocyte Ca2+ oscillation frequency in rats and prevented cardiac hypertrophy in mice.","authors":"Y Wang, H Y Wang, Z K Yuan, X N Zhao, J X Wang, Z X Zhang","doi":"","DOIUrl":"","url":null,"abstract":"Aim: To study the effects of quercetin (Que) on myocardial excitation-contraction coupling and cardiac remodeling.Methods: Left ventricles and femoral arteries of rats were cannulated for hemodynamic recording. Mouse cardiac hypertrophy was induced by abdominal aortic coarctation (AAC). Cultured myocardial cells in neonatal rats were loaded with Fura 2-AM. The intracellular calcium ([Ca2+]i) and spontaneous [Ca2+]i oscillations ([Ca2+]i-SO) were tested by AR-CM-MIC cation measurement system.Results: Que 3 or 25 mg.kg-1 i.v. in rats decreased heart rate from (420 +/- 19) to (390 +/- 15) and (314 +/- 18) beat.min-1, respectively, companied with very modest changes in both left ventricular pressures (LVP) and its differential dpLV/dtmax. Que 10, 50, 250 mumol.L-1 concentration-dependently slowed the frequency of [Ca2+]i-SO in cultured myocardial cells from (26 +/- 4) to (25 +/- 3), (18 +/- 4), and (12 +/- 3) time.min-1, respectively, but did not change their resting [Ca2+]i or amplitudes of [Ca2+]i-SO. Similarly, the increases in frequency of [Ca2+]i-SO caused by either isoproterenol (Iso) or ouabain (Oua) were prevented by Que 100 mumol.L-1, while the simultaneous increases in amplitude of [Ca2+]i-SO remained. Besides, [Ca2+]i rises excited by angiotensin II (Ang II) but not high [K+]o were prevented by Que 100 mumol.L-1. Daily administration of Que 120 mg.kg-1 i.g. for 5 d markedly prevented the cardiac hypertrophy in AAC mice, without effects on the ventricular mass to body weight ratio (VM/BW) in sham-operated mice.Conclusion: Que decreased myocardial [Ca2+]i-oscillation frequency and prevented cardiac remodeling, but had no direct effect on cardiac excitation-contraction coupling.","PeriodicalId":24002,"journal":{"name":"Zhongguo yao li xue bao = Acta pharmacologica Sinica","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1999-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21532717","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Gene therapy for human hepatocellular carcinoma with cytosine deaminase gene and prodrug flucytosine. 胞嘧啶脱氨酶基因与前药氟胞嘧啶治疗人肝癌的研究。

Zhongguo yao li xue bao = Acta pharmacologica Sinica Pub Date : 1999-05-01

H Z Lü, D Z Wu, Y L Wan, B Gu, H Z Gao, Y Q Liang, J X Wang

{"title":"Gene therapy for human hepatocellular carcinoma with cytosine deaminase gene and prodrug flucytosine.","authors":"H Z Lü, D Z Wu, Y L Wan, B Gu, H Z Gao, Y Q Liang, J X Wang","doi":"","DOIUrl":"","url":null,"abstract":"Aim: To investigate the antitumor effects of cytosine deaminase (CD) gene in combination with prodrug flucytosine (Flu, 5-fluorocytosine) on human hepatocellular carcinoma.Methods: CD gene was transduced into human hepatocellular carcinoma cell line SMMC7721 with retroviral method and the cytotoxicity of Flu on the tumor cells was assayed in vitro with clonogenic techniques. The xenograft tumor model in nude mice was used to study in vivo therapeutic effects of CD gene/Flu system against human hepatocellular carcinoma.Results: CD gene/Flu system had significant antitumor activities on human hepatocellular carcinoma cells in vitro and in nude mice. The antitumor activities of Flu 500 mg.kg-1 on hepatocellular carcinoma xenografts in nude mice were more potent than those of 5-fluouracil 10 mg.kg-1. CD gene/Flu system possessed bystander killing effects on hepatocellular carcinoma xenografts in nude mice.Conclusion: The experiment demonstrates the potential value of the CD gene/Flu system in the treatment of human hepatocellular carcinoma.","PeriodicalId":24002,"journal":{"name":"Zhongguo yao li xue bao = Acta pharmacologica Sinica","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1999-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21532720","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ro 31-8220 inhibits release of interleukin-1 and interleukin-6 from mouse peritoneal macrophages induced by fibrin fibrinogen degradation products. Ro 31-8220抑制纤维蛋白原降解产物诱导的小鼠腹腔巨噬细胞释放白细胞介素-1和白细胞介素-6。

Zhongguo yao li xue bao = Acta pharmacologica Sinica Pub Date : 1999-05-01

B Zhou, J P Zhang, Z L Hu, Y X Tan, D H Qian

引用次数: 0