Apoptosis and necrosis induced by sulfur mustard in Hela cells.

J Sun, Y X Wang, M J Sun
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引用次数: 0

Abstract

Aim: To study the apoptotic effect of sulfur mustard (SM) on Hela cells.

Methods: Exponentially growing Hela cells were treated with SM at various concentrations for 3 h, then apoptosis was examined by electron-microscope, DNA gel electrophoresis, and flow cytometry.

Results: SM 1 mumol.L-1 arrested cell growth. After treatment with SM 10-100 mumol.L-1, cells were mainly blocked at G1-phase with apoptosis. Agarose gel electrophoresis of DNA from cells treated with SM revealed "DNA Ladder." About 33% of the Hela cells showed apoptosis 12 h after 3-h treatment with SM 100 mumol.L-1 as determined by flow cytometry and the S-phase cells were more susceptible. However, SM 1000 mumol.L-1 caused marked necrosis in Hela cells.

Conclusion: SM caused 2 distinct forms of cell death, apoptosis or necrosis, in Hela cells in a concentration-dependent manner.

硫芥诱导Hela细胞凋亡和坏死。
目的:研究硫芥(SM)对Hela细胞凋亡的影响。方法:用不同浓度的SM处理指数生长的Hela细胞3 h,用电镜、DNA凝胶电泳、流式细胞术观察细胞凋亡情况。结果:sm1mumol;L-1抑制细胞生长。经SM 10-100 μ mol处理后。L-1时,细胞主要阻滞在g1期并发生凋亡。经SM处理的细胞DNA琼脂糖凝胶电泳显示“DNA阶梯”。sm100 mumol作用3 h后12 h,约33%的Hela细胞出现凋亡。流式细胞术检测的L-1和s期细胞更敏感。然而,sm1000 μ mol。L-1引起Hela细胞明显坏死。结论:SM可引起Hela细胞凋亡和坏死两种不同形式的细胞死亡,并呈浓度依赖性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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