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Zeitschrift fur Naturforschung. Section C, Biosciences最新文献

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Isolation of the adducts of platinum complexes and nucleic acid bases on the Dowex 50 W column. 铂配合物加合物和核酸碱基在Dowex 50w色谱柱上的分离。
Zeitschrift fur Naturforschung. Section C, Biosciences Pub Date : 1984-11-01
R Oliński, Z Walter
{"title":"Isolation of the adducts of platinum complexes and nucleic acid bases on the Dowex 50 W column.","authors":"R Oliński,&nbsp;Z Walter","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The method presented here enables the isolation of three monofunctional adducts. These are: platination of cytosine, adenine and guanosine. The three detected bifunctional complexes were: guanine N7 to N7, adenine N7 to N7 and adenine N7 to N1. A mixed bifunctional complex of guanine and adenosine and the product of polymerization of three adenines and two moieties of cis DDP were also detected. In the peak eluted separately from the guanine standard, no Pt was detected. The sample eluted in this peak had UV spectrum different from the standard and may represent the degraded product of guanine and polymerized Pt.</p>","PeriodicalId":23914,"journal":{"name":"Zeitschrift fur Naturforschung. Section C, Biosciences","volume":"39 11-12","pages":"1052-6"},"PeriodicalIF":0.0,"publicationDate":"1984-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17601337","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[Stimulation of immunoreactivity against endogenous retroviruses and protection against leukemia in aged AKR mice after vaccination with antibodies to viral surface components. The role of antibodies to p15(E)]. 接种病毒表面成分抗体后，刺激老年AKR小鼠对内源性逆转录病毒的免疫反应性和对白血病的保护作用。p15(E)抗体的作用[j]。
Zeitschrift fur Naturforschung. Section C, Biosciences Pub Date : 1984-11-01
H Schwarz, H J Thiel, K J Weinhold, D P Bolognesi, W Schäfer
{"title":"[Stimulation of immunoreactivity against endogenous retroviruses and protection against leukemia in aged AKR mice after vaccination with antibodies to viral surface components. The role of antibodies to p15(E)].","authors":"H Schwarz,&nbsp;H J Thiel,&nbsp;K J Weinhold,&nbsp;D P Bolognesi,&nbsp;W Schäfer","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Antibody against viral gp71 is effective therapeutically for high leukemic AKR mice if injected immediately after birth. No corresponding effect could be observed after inoculation later in life when the endogenous virus burden is already high. However, if antibody treatment was supplemented by the injection of p15(E) antibody, a therapeutic effect was observed even in older mice first treated at an age of 21/2 months. Those mice produced antibodies against viral surface proteins and appeared to be able to survive longer than control mice. Thus p15(E) antibody might be able to overcome retroviral associated immuno-deficiency. This therapy may have implications for the treatment of the apparently retroviral induced acquired immunodeficiency syndrome (AIDS) of man.</p>","PeriodicalId":23914,"journal":{"name":"Zeitschrift fur Naturforschung. Section C, Biosciences","volume":"39 11-12","pages":"1199-202"},"PeriodicalIF":0.0,"publicationDate":"1984-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17588721","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Inhibitors of calmodulin-dependent phosphorylation simultaneously inhibit calcium uptake and calcium-dependent ATPase activity in skeletal muscle sarcoplasmic reticulum and transiently induce calcium release. 钙调素依赖性磷酸化抑制剂同时抑制骨骼肌肌浆网钙摄取和钙依赖性atp酶活性，并短暂诱导钙释放。
Zeitschrift fur Naturforschung. Section C, Biosciences Pub Date : 1984-11-01 DOI: 10.1515/znc-1984-11-1235
W Hasselbach, A Migala
{"title":"Inhibitors of calmodulin-dependent phosphorylation simultaneously inhibit calcium uptake and calcium-dependent ATPase activity in skeletal muscle sarcoplasmic reticulum and transiently induce calcium release.","authors":"W Hasselbach,&nbsp;A Migala","doi":"10.1515/znc-1984-11-1235","DOIUrl":"https://doi.org/10.1515/znc-1984-11-1235","url":null,"abstract":"<p><p>Under adequate experimental conditions calmodulin antagonists like compound 48/80 do not dissociate calcium uptake from the calcium-dependent ATP hydrolysis of skeletal muscle sarcoplasmic reticulum membranes but simultaneously inhibit both processes. Apart from the agent's pump inhibiting effect, they interact with the caffeine sensitive calcium channel in the sarcoplasmic reticulum causing a rapid transient calcium release.</p>","PeriodicalId":23914,"journal":{"name":"Zeitschrift fur Naturforschung. Section C, Biosciences","volume":"39 11-12","pages":"1189-91"},"PeriodicalIF":0.0,"publicationDate":"1984-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1515/znc-1984-11-1235","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17305438","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 4
Metabolic effects of direct current stimulation on cultured vascular smooth muscle cells. 直流电刺激对培养血管平滑肌细胞代谢的影响。
Zeitschrift fur Naturforschung. Section C, Biosciences Pub Date : 1984-11-01 DOI: 10.1515/znc-1984-11-1225
H Heinle, G Sigg, A Reich, K U Thiedemann
{"title":"Metabolic effects of direct current stimulation on cultured vascular smooth muscle cells.","authors":"H Heinle,&nbsp;G Sigg,&nbsp;A Reich,&nbsp;K U Thiedemann","doi":"10.1515/znc-1984-11-1225","DOIUrl":"https://doi.org/10.1515/znc-1984-11-1225","url":null,"abstract":"<p><p>Vascular smooth muscle cells from rabbit arteries were grown in tissue culture and stimulated by DC impulses (1 mA, 1 V, 10 Hz, 1 ms/imp). Scanning microscopic examination disclosed that in stimulated cultures the cell surface was enlarged by numerous microvilli. This was interpreted as being indicative of an increase in cell activity. Cellular metabolism was characterized by analyzing the incubation medium for glucose, glutamate/glutamine, and lactate. When compared to unstimulated controls, stimulation caused an increase in the uptake of glucose and glutamine as well as an increased lactate production. The enhancing effect on metabolism was prevented when the \"calcium antagonist\" verapamil was present (5 X 10(-6) M). Although the exact mechanism by which DC stimulation influences the cells remains obscure, this finding indicates an important mediating role of Ca2+ ions.</p>","PeriodicalId":23914,"journal":{"name":"Zeitschrift fur Naturforschung. Section C, Biosciences","volume":"39 11-12","pages":"1141-4"},"PeriodicalIF":0.0,"publicationDate":"1984-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1515/znc-1984-11-1225","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17588839","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 2
Effect of extracellular alkali metal salts on the electric parameters of human erythrocytes in normal and pathological conditions (homozygous beta-thalassemia). 细胞外碱金属盐对正常和病理(纯合子-地中海贫血)人红细胞电参数的影响。
Zeitschrift fur Naturforschung. Section C, Biosciences Pub Date : 1984-11-01 DOI: 10.1515/znc-1984-11-1230
C Ballario, A Bonincontro, C Cametti, A Rosi, L Sportelli
{"title":"Effect of extracellular alkali metal salts on the electric parameters of human erythrocytes in normal and pathological conditions (homozygous beta-thalassemia).","authors":"C Ballario,&nbsp;A Bonincontro,&nbsp;C Cametti,&nbsp;A Rosi,&nbsp;L Sportelli","doi":"10.1515/znc-1984-11-1230","DOIUrl":"https://doi.org/10.1515/znc-1984-11-1230","url":null,"abstract":"<p><p>The conductivity of human erythrocyte cells dispersed in various uni-univalent electrolyte solutions (NaCl, KCl, LiCl, CsCl; 0.15 M) have been measured in the frequency range from 10 KHz to 100 MHz at five temperatures between 5 and 45 degrees C. The results were analyzed in the light of the theory of conductivity polarization of a suspension of ellipsoidal particles-covered with two confocal shells. Differences in the electrical parameters of the membrane between normal and homozygous beta-thalassemic cells have been evidentiated.</p>","PeriodicalId":23914,"journal":{"name":"Zeitschrift fur Naturforschung. Section C, Biosciences","volume":"39 11-12","pages":"1163-9"},"PeriodicalIF":0.0,"publicationDate":"1984-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1515/znc-1984-11-1230","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17588841","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 18
Stimulation of brain synaptosome-associated adenylate cyclase by acidic phospholipids. 酸性磷脂对脑突触体相关腺苷酸环化酶的刺激。
Zeitschrift fur Naturforschung. Section C, Biosciences Pub Date : 1984-11-01 DOI: 10.1515/znc-1984-11-1237
S Tsakiris
{"title":"Stimulation of brain synaptosome-associated adenylate cyclase by acidic phospholipids.","authors":"S Tsakiris","doi":"10.1515/znc-1984-11-1237","DOIUrl":"https://doi.org/10.1515/znc-1984-11-1237","url":null,"abstract":"<p><p>Phosphatidylserine (PS), phosphatidylinositol (PIN) or phosphatidylglycerol (PGL) incubated with synaptosomal plasma membranes (SPM) of dog brain, stimulated adenylate cyclase. The enzyme activity showed a dramatic increase at around 1.6 mumol PS/mg protein, while use of higher concentrations led to inhibition of the activity with respect to the maximal percentage of stimulation. Moreover, PS stimulated the dopamine-sensitive adenylate cyclase. Solubilization of SPM by the detergent Lubrol-PX did not affect the enzyme activation induced by dopamine. The solubilization, also, showed that the enzyme activity does not change at any PS, PIN or PGL concentration used. These results indicate that acidic phospholipids do not directly act on adenylate cyclase, but indirectly, affecting the membrane fluidity probably. Such modifications of interactions through lipid-protein(s) of adenylate cyclase may have implications to physiological responses to hormones or/and neurotransmitters in the central nervous system.</p>","PeriodicalId":23914,"journal":{"name":"Zeitschrift fur Naturforschung. Section C, Biosciences","volume":"39 11-12","pages":"1196-8"},"PeriodicalIF":0.0,"publicationDate":"1984-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1515/znc-1984-11-1237","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17588843","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
Haemolytic properties of cereal 5-n-alk(en)ylresorcinols. 谷物5-n-烷基间苯二酚的溶血特性。
Zeitschrift fur Naturforschung. Section C, Biosciences Pub Date : 1984-11-01 DOI: 10.1515/znc-1984-11-1223
A Kozubek
{"title":"Haemolytic properties of cereal 5-n-alk(en)ylresorcinols.","authors":"A Kozubek","doi":"10.1515/znc-1984-11-1223","DOIUrl":"https://doi.org/10.1515/znc-1984-11-1223","url":null,"abstract":"<p><p>The haemolytic activity of 5-n-alk(en)ylresorcinols is temperature dependent and correlated to their transition temperatures. The values of the parameters describing the 5-n-alk(en)ylresorcinol-induced red blood cell lysis indicate strong affinity of the compounds to the membrane and their high lytic capacity. The affinity of the compounds for the membrane decrease with the increasing quantity of the molecules incorporated into the erythrocyte membrane and is much higher for saturated resorcinols than unsaturated ones. The amount of 5-n-alk(en)ylresorcinol molecules bound to the membrane at a hundred percent lysis is about eighty times and eleven times (for alkyl and alkenyl derivatives respectively) higher than at zero percent lysis. Estimated free energy of erythrocyte lysis was similar for alkyl and alkenyl derivatives of resorcinol provided the preparation of the resorcinolic suspensions above their transition temperatures.</p>","PeriodicalId":23914,"journal":{"name":"Zeitschrift fur Naturforschung. Section C, Biosciences","volume":"39 11-12","pages":"1132-6"},"PeriodicalIF":0.0,"publicationDate":"1984-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1515/znc-1984-11-1223","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17588836","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 7
Effects of vitamin D3 on in vivo labelling of chick skeletal muscle proteins with [3H]leucine. 维生素D3对鸡骨骼肌蛋白[3H]亮氨酸体内标记的影响。
Zeitschrift fur Naturforschung. Section C, Biosciences Pub Date : 1984-09-01 DOI: 10.1515/znc-1984-9-1025
A R de Boland, R L Boland
{"title":"Effects of vitamin D3 on in vivo labelling of chick skeletal muscle proteins with [3H]leucine.","authors":"A R de Boland,&nbsp;R L Boland","doi":"10.1515/znc-1984-9-1025","DOIUrl":"https://doi.org/10.1515/znc-1984-9-1025","url":null,"abstract":"<p><p>The effects of the administration of a single oral dose of vitamin D3 to rachitic chicks on the in vivo incorporation of [3H]leucine to proteins of skeletal muscle subcellular fractions was studied. A significant stimulation (50%) of labelling of mitochondrial proteins could be seen. The sterol affected the labelling of sarcoplasmic reticulum (20%) and contractile proteins (10%) to a lesser extent. These results confirm previous observations which implicate vitamin D3 in the synthesis of mitochondrial proteins.</p>","PeriodicalId":23914,"journal":{"name":"Zeitschrift fur Naturforschung. Section C, Biosciences","volume":"39 9-10","pages":"1015-6"},"PeriodicalIF":0.0,"publicationDate":"1984-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1515/znc-1984-9-1025","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17163580","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 3
Binding of the fluorescent dye 8-anilinonaphthalene 1-sulfonic acid to the native and pressure dissociated beta 2-dimer of tryptophan synthase from Escherichia coli. 荧光染料8-苯胺萘磺酸与大肠杆菌色氨酸合成酶的天然和压力解离- 2二聚体的结合。
Zeitschrift fur Naturforschung. Section C, Biosciences Pub Date : 1984-09-01 DOI: 10.1515/znc-1984-9-1023
T Seifert, P Bartholmes, R Jaenicke
{"title":"Binding of the fluorescent dye 8-anilinonaphthalene 1-sulfonic acid to the native and pressure dissociated beta 2-dimer of tryptophan synthase from Escherichia coli.","authors":"T Seifert,&nbsp;P Bartholmes,&nbsp;R Jaenicke","doi":"10.1515/znc-1984-9-1023","DOIUrl":"https://doi.org/10.1515/znc-1984-9-1023","url":null,"abstract":"<p><p>The beta 2-dimer of tryptophan synthase from Escherichia coli exhibits weak binding of 8-anilinonaphthalene-1-sulfonic acid (ANS). Titrating the dye at 0.2 mM concentration with the apo-beta 2-dimer at atmospheric pressure causes increased fluorescence emission at 480 nm (lambda exc = 380 nm), corresponding to unspecific binding of the ligand to hydrophobic residues. Increasing hydrostatic pressure affects ANS binding. Up to 700 bar, a sigmoidal increase of ANS fluorescence reflects an increase in hydrophobic surface area, probably caused by subunit dissociation. At approximately 1 kbar, a maximum is reached; beyond this value, pressure competes with ligand binding causing fluorescence emission to be decreased again. Pressure release leads to a drastic fluorescence enhancement, ascribed to ANS binding to the partially and reversibly denatured enzyme. Plotting the total fluorescence enhancement vs. pressure yields a profile which parallels the pressure dependent dimer in equilibrium monomer transition monitored by subunit hybridization (T. Seifert, P. Bartholmes, and R. Jaenicke, Biochemistry, in press).</p>","PeriodicalId":23914,"journal":{"name":"Zeitschrift fur Naturforschung. Section C, Biosciences","volume":"39 9-10","pages":"1008-11"},"PeriodicalIF":0.0,"publicationDate":"1984-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1515/znc-1984-9-1023","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17452483","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 2
[New isolation procedure for swine kidney acylase. Kinetics of Co2+, Mn2+, Ni2+ and Cd2+-enzymes]. 猪肾酰化酶分离新方法。Co2+， Mn2+， Ni2+和Cd2+-酶的动力学[j]。
Zeitschrift fur Naturforschung. Section C, Biosciences Pub Date : 1984-09-01
I Gilles, H G Löffler, F Schneider
{"title":"[New isolation procedure for swine kidney acylase. Kinetics of Co2+, Mn2+, Ni2+ and Cd2+-enzymes].","authors":"I Gilles,&nbsp;H G Löffler,&nbsp;F Schneider","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>A new procedure for the isolation of highly purified acylamino acid amidohydrolase from hog kidney is described which allows the preparation of the enzyme with a recovery of about 45%, a 200 fold purification and a spec. activity of 350-500 U. The essential Zn2+ of the enzyme was exchanged for Co2+, Ni2+, Mn2+ and Cd2+, and the kinetic parameters KM, kcat and kcat/KM of the different enzyme species for a series of acetyl-L-amino acids were determined.</p>","PeriodicalId":23914,"journal":{"name":"Zeitschrift fur Naturforschung. Section C, Biosciences","volume":"39 9-10","pages":"1017-20"},"PeriodicalIF":0.0,"publicationDate":"1984-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17571034","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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