Isolation of the adducts of platinum complexes and nucleic acid bases on the Dowex 50 W column.

Abstract

The method presented here enables the isolation of three monofunctional adducts. These are: platination of cytosine, adenine and guanosine. The three detected bifunctional complexes were: guanine N7 to N7, adenine N7 to N7 and adenine N7 to N1. A mixed bifunctional complex of guanine and adenosine and the product of polymerization of three adenines and two moieties of cis DDP were also detected. In the peak eluted separately from the guanine standard, no Pt was detected. The sample eluted in this peak had UV spectrum different from the standard and may represent the degraded product of guanine and polymerized Pt.