Zeitschrift fur Naturforschung. Section C, Biosciences最新文献

MTD approach to quantitative structure-activity relationships for cardiotonic steroids. 强心剂类固醇定量构效关系的MTD方法。

Zeitschrift fur Naturforschung. Section C, Biosciences Pub Date : 1985-11-01

M Bohl, Z Simon

引用次数: 0

Tumor inhibition by ferricenium complexes: systemic effect in vivo and cell growth inhibition in vitro. 铁配合物的肿瘤抑制作用:体内的全身效应和体外的细胞生长抑制。

Zeitschrift fur Naturforschung. Section C, Biosciences Pub Date : 1985-11-01

P Köpf-Maier

{"title":"Tumor inhibition by ferricenium complexes: systemic effect in vivo and cell growth inhibition in vitro.","authors":"P Köpf-Maier","doi":"","DOIUrl":"","url":null,"abstract":"The antiproliferative activity of divers ferricenium complexes [Cp2Fe]+X-(X- = [CC13COO]- X CC13COOH (I); X- = [CC13COOH]- X 2 CC13COOH (II); X- = 1/2 [C13FeOFeC13]2- (III); X- = [FeC14]- (IV); X- = [2,4,6-(NO2)3C6H2O]- (V)) was investigated against solid, subcutaneously growing Ehrlich ascites tumor (EAT) in vivo as well as against EAT cells cultivated in vitro as permanent suspension culture. In vivo, triple intraperitoneal injections of the complexes II (3 X 200 mg/kg), III (3 X 100, 140, 180 mg/kg) or IV (3 X 160 mg/kg) markedly suppressed tumor development thus that the sizes of treated tumors were reduced to 42-48% related to control tumors (100%); these results point to the systemic character of the antitumor action by ferricenium complexes in vivo. In vitro, all ferricenium complexes inhibited cellular proliferation to an equal extent; application of 10(-5) M diminished the increase in cell number by 20-40%, application of 10(-4) M resulted in a total cessation of cellular proliferation. In comparison to cis-diamminedichloroplatinum(II), the cell growth-inhibiting effect of ferricenium complexes was less pronounced and required 10- to 50-fold higher concentration levels to evoke equivalent cytostasis.","PeriodicalId":23914,"journal":{"name":"Zeitschrift fur Naturforschung. Section C, Biosciences","volume":"40 11-12","pages":"843-6"},"PeriodicalIF":0.0,"publicationDate":"1985-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14948936","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Further observations on periodicities of nucleotide occurrences in natural DNA's. 对天然DNA中核苷酸出现周期的进一步观察。

Zeitschrift fur Naturforschung. Section C, Biosciences Pub Date : 1985-11-01

N Burr Furlong, K Marien

{"title":"Further observations on periodicities of nucleotide occurrences in natural DNA's.","authors":"N Burr Furlong, K Marien","doi":"","DOIUrl":"","url":null,"abstract":"There are non-random features in the occurrences of nucleotides in the DNA's of certain organisms which are detectable by statistical analyses of the entire sequence. Earlier, using the bacteriophage Phi-X 174 DNA sequence, we had reported that the self-information values for one type of dinucleotide association showed a marked periodicity when their autocorrelation coefficients were graphed. A similar, but computationally simpler, analysis has been developed which gives a comparable indication of periodicity. The difference, in average autocorrelation coefficients obtained with this analysis, between the peak values and all others has been used as an index to compare the extent of periodic non-randomness for a series of natural DNA sequences and for various artificial sequences. Calculations show that triplet periodicity, the relationship between dinucleotides separated by a single nucleotide, is characteristic only of the natural sequences of certain filamentous phages and is not found prominently in any other DNA analyzed (including sequences of similar length from plasmids, yeast, bacteria and higher animals). By shuffling nucleotides in a given sequence or by substituting selected nucleotides to alter various positions in both periodic and aperiodic sequences, we have found that an excess or deficiency of a given nucleotide at one of the three positions in a triplet reading frame can simulate the periodic characteristic. Thus, it appears that this global statistical analysis detects the tendency for single-strand phages to utilize a specific nucleotide, rather than one randomly selected, to constitute codons.","PeriodicalId":23914,"journal":{"name":"Zeitschrift fur Naturforschung. Section C, Biosciences","volume":"40 11-12","pages":"854-7"},"PeriodicalIF":0.0,"publicationDate":"1985-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14072893","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Growth response of mouse lymphoma cells to low concentrations of mercuric chloride. 小鼠淋巴瘤细胞对低浓度氯化汞的生长反应。

Zeitschrift fur Naturforschung. Section C, Biosciences Pub Date : 1985-11-01

S P Jost, J Cole, B C Goodwin

引用次数: 0

High yields of DNA-transfer into mouse L-cells by electropermeabilization. 高产量的dna通过电渗透转移到小鼠l细胞。

Zeitschrift fur Naturforschung. Section C, Biosciences Pub Date : 1985-11-01

H Stopper, U Zimmermann, E Wecker

引用次数: 0

[Mammalian spermatogenesis as a biological indicator for ionizing radiation]. [作为电离辐射生物学指标的哺乳动物精子发生]。

Zeitschrift fur Naturforschung. Section C, Biosciences Pub Date : 1985-11-01

U Hacker-Klom, E M Meier, W Göhde

引用次数: 0

Biochemical characterization of propylglyoxal bis(guanylhydrazone). Facile synthesis of monoalkylglyoxal bis(guanylhydrazones). 丙基乙二醛双胍腙的生化表征。单烷基乙二醛双胍腙的简易合成。

Zeitschrift fur Naturforschung. Section C, Biosciences Pub Date : 1985-11-01

H Elo, R Laine, L Alhonen-Hongisto, J Jänne, I Mutikainen, P Lumme

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Female urinary chemosignals influence scent-marking behavior in male Mongolian gerbils (Meriones unguiculatus). 雌性尿液化学信号影响雄性蒙古沙鼠的气味标记行为。

Zeitschrift fur Naturforschung. Section C, Biosciences Pub Date : 1985-11-01

B Probst

引用次数: 0

A disappearance of a 24-kDa acid-soluble protein from liver chromatin of normal and starved hens following D-galactosamine administration. 在给药d-半乳糖胺后，正常和饥饿母鸡肝染色质中一种24kda酸溶性蛋白消失。

Zeitschrift fur Naturforschung. Section C, Biosciences Pub Date : 1985-11-01

J Palyga

引用次数: 0

Reaction of fluorescein isothiocyanate with thiol and amino groups of sarcoplasmic ATPase. 异硫氰酸荧光素与肌浆atp酶的巯基和氨基的反应。

Zeitschrift fur Naturforschung. Section C, Biosciences Pub Date : 1985-11-01

G Swoboda, W Hasselbach

{"title":"Reaction of fluorescein isothiocyanate with thiol and amino groups of sarcoplasmic ATPase.","authors":"G Swoboda, W Hasselbach","doi":"","DOIUrl":"","url":null,"abstract":"Several model compounds containing thiol and/or amino groups (mercaptoethanol, glutathione, cysteine, ethanolamine, glycine) were studied with respect to their reactivity towards fluorescein isothiocyanate (followed spectrophotometrically at 504 and 412 nm), stability of product and long-wave absorption maximum of the fluorescein residue attached. Thiol groups reacted by far more readily than amino groups. A specific effect was observed with cysteine, indicating an intramolecular transfer of the fluorescein residue from SH to NH2. With sarcoplasmic vesicles both types of reactions were observed. The ratio of products, which can be distinguished by their different stabilities and absorption spectra, depended on the absence or presence of detergents. While with native vesicles the NH2 reaction predominated, with vesicles solubilized with sodium dodecylsulfate, octaethyleneglycol mono-n-dodecyl ether or 1-0-tetradecyl-propanediol-(1,3)-3-phosphorylcholine the SH reaction became prevailing. Already 0.35 mg sodium dodecylsulfate per mg protein were sufficient to give rise to dithiourethane formation exclusively. Excess fluorescein isothiocyanate reacted with several thiol groups of dodecylsulfate-solubilized vesicles. In the presence of ATP binding of fluorescein isothiocyanate to native vesicles was significantly reduced. Total blockage of the vesicular SH groups with N-ethyl-maleimide led to preparations that reacted with fluorescein isothiocyanate much more slowly, compared to native vesicles. Octaethyleneglycol mono-n-dodecyl ether or 1-0-tetradecyl-propanediol-(1,3)-3-phosphorylcholine in the assay accelerated the thioureide formation from N-ethylmaleimide modified vesicles, whereas sodium dodecylsulfate prevented it almost completely. Our results support the suggestion that one or several thiol groups in vicinity of the highly reactive lysyl residue might play a role in the fast specific reaction, which is only observed with intact native vesicles.","PeriodicalId":23914,"journal":{"name":"Zeitschrift fur Naturforschung. Section C, Biosciences","volume":"40 11-12","pages":"863-75"},"PeriodicalIF":0.0,"publicationDate":"1985-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14072896","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0