Zeitschrift fur Naturforschung. Section C, Biosciences最新文献_第2页

Reculturing of cells from primary CFU-C colonies. 原代CFU-C菌落细胞的再培养。

Zeitschrift fur Naturforschung. Section C, Biosciences Pub Date : 1985-11-01

G E Hübner, F Ali-Osman, M Kastner, C Papadimitriou, H R Maurer

{"title":"Reculturing of cells from primary CFU-C colonies.","authors":"G E Hübner, F Ali-Osman, M Kastner, C Papadimitriou, H R Maurer","doi":"","DOIUrl":"","url":null,"abstract":"This study was aimed at investigating whether cells of CFU-C derived colonies could form secondary colonies. Bone marrow cultures of volumes of agar medium between 25 microliter and 75 microliter contained in glass capillaries were stimulated with mouse lung-conditioned medium (MLCM) containing granulocyte/macrophage colony-stimulating factor (GM-CSF). Agar gels with colonies of up to greater than or equal to 20 were blown out into identical culture medium, completely dispersed on a whirl-mix to single cell suspensions, and used for establishing secondary agar cultures. In these secondary cultures considerable numbers of secondary granulocytic, mixed granulocytic/macrophage and macrophage colonies as well as numerous clusters arose. In contrast, when single colonies were recultured, only few secondary cell aggregates were formed. When primary cultures containing up to greater than or equal to 20 cell aggregates were used for serial reculture at intermittent intervals of 3 and 4 days, a 2-7-fold increase of colony-forming cells was found in tertiary cultures as was monitored by 7 day colony counts. And by use of different kinds of CSF-containing media, an over 4-fold increase of secondary over primary colonies was obtained with bovine lung-conditioned medium (BLCM) in primary and L-cell-conditioned medium (LCCM) in secondary 7 day cultures. Primary capillary cultures were found to be devoid of CFU-S. Also, setting up bone marrow cultures in petri dishes and stimulating with MLCM, growth of primary as well as secondary colonies was obtained. The results indicate some self-renewal potential of CFU-C in vitro.","PeriodicalId":23914,"journal":{"name":"Zeitschrift fur Naturforschung. Section C, Biosciences","volume":"40 11-12","pages":"891-7"},"PeriodicalIF":0.0,"publicationDate":"1985-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14996277","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Formation and decay of the vanadate complex of the sarcoplasmic reticulum calcium transport protein. 肌浆网钙转运蛋白钒酸盐复合物的形成和衰变。

Zeitschrift fur Naturforschung. Section C, Biosciences Pub Date : 1985-11-01

P Medda, W Hasselbach

引用次数: 0

Thyroxine induced transformation in sarcoplasmic reticulum of rabbit soleus and psoas muscles. 甲状腺素诱导兔比目鱼肌和腰肌肌浆网转化。

Zeitschrift fur Naturforschung. Section C, Biosciences Pub Date : 1985-09-01 DOI: 10.1515/znc-1985-9-1025

M T Nunes, A C Bianco, A Migala, B Agostini, W Hasselbach

{"title":"Thyroxine induced transformation in sarcoplasmic reticulum of rabbit soleus and psoas muscles.","authors":"M T Nunes, A C Bianco, A Migala, B Agostini, W Hasselbach","doi":"10.1515/znc-1985-9-1025","DOIUrl":"https://doi.org/10.1515/znc-1985-9-1025","url":null,"abstract":"The properties of the sarcoplasmic reticulum membranes isolated from slow-twitch type I soleus and fast-twitch type II psoas muscles of control and thyroxine treated rabbits were comparatively studied. Membrane yield, maximal calcium storing capacity, ATP-supported calcium uptake, calcium-dependent ATPase activity and calcium-dependent phosphoprotein formation were found to be 3-10 fold higher in psoas than in soleus preparations. Membrane yield, calcium-dependent ATPase activity, ATP-supported calcium transport and calcium-dependent phosphoprotein are at least twice enhanced in the membranes from soleus muscles of animals treated for 14-21 days with thyroxine. The corresponding capacities of the membranes from psoas muscles are not further augmented by the same thyroxine treatment. The maximal calcium storing capacity of the psoas membranes is their sole specific property which is significantly increased. The changes in the properties of the soleus muscles' sarcoplasmic reticulum membranes are engendered by an increase from 5 to 30-50% in the number of type II fibres. Since the calcium transporting properties of the sarcoplasmic reticulum membranes from type II fibres qualitatively differ from those of type I fibres, thyroxine does not only affect quantitative but also qualitative parameters of the muscles' sarcoplasmic reticulum membrane system.","PeriodicalId":23914,"journal":{"name":"Zeitschrift fur Naturforschung. Section C, Biosciences","volume":"40 9-10","pages":"726-34"},"PeriodicalIF":0.0,"publicationDate":"1985-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1515/znc-1985-9-1025","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14069967","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 32

On the active principles of the spurge family (Euphorbiaceae). XI. [1] The skin irritant and tumor promoting diterpene esters of Euphorbia tirucalli L. originating from South Africa. 论大戟科植物的活性成分。西[1]南非大戟(Euphorbia tirucalli L.)的皮肤刺激性和促肿瘤二萜酯。

Zeitschrift fur Naturforschung. Section C, Biosciences Pub Date : 1985-09-01 DOI: 10.1515/znc-1985-9-1008

G Fürstenberger, E Hecker

{"title":"On the active principles of the spurge family (Euphorbiaceae). XI. [1] The skin irritant and tumor promoting diterpene esters of Euphorbia tirucalli L. originating from South Africa.","authors":"G Fürstenberger, E Hecker","doi":"10.1515/znc-1985-9-1008","DOIUrl":"https://doi.org/10.1515/znc-1985-9-1008","url":null,"abstract":"The irritant and tumor-promoting constituents of latex of Euphorbia tirucalli L. originating from South Africa were isolated. They were identified as irritant ingenane and tigliane type diterpene esters derived from unsaturated aliphatic acids and acetic acid and the polyfunctional diterpene parent alcohols 4-deoxyphorbol, phorbol and ingenol, respectively. The irritant and tumor-promoting esters of 4-deoxyphorbol are predominant and were fully characterized chemically and biologically. They are positionally isomeric 12,13-acylates, acetates e.g. Euphorbiafactors Ti1-Ti4. As acyl groups they carry homologous, highly unsaturated aliphatic acids of the general structure CH3-(CH2)m-(CH = CH)n-COOH (m = 2,4; n = 1,2, 3,4,5; N = 2n + m + 2). Corresponding diesters of 4-deoxy-4 alpha-phorbol are also present which are biologically inactive. Comparison of structures and biological activities of 12,13-diesters of 4-deoxyphorbol indicates that--for a distinct total number of C-atoms (N) in the acyl moiety--an increasing number of conjugated double bonds (n) may increase the irritant but decrease the tumor-promoting activity. Replacement of the hydroxyl function at C-4 (phorbol-12,13-diesters) by hydrogen (corresponding 4-deoxyphorbol-12,13-diesters) does not essentially alter biological activities. Epimerization of 4-deoxyphorbol-12,13-diesters at C-4 abolishes biological activities. The specific chemical properties demonstrated for the diterpene ester irritants contained in the latex of E. tirucalli and hence in all plant parts may be useful in trials to abolish the potential risk of cancer involved especially in occupational mass production and handling of the plant. Some of the structure activity relations of the Euphorbia factors isolated made them excellent tools in experimental cancer research for the analysis of mechanisms of tumorigenesis.","PeriodicalId":23914,"journal":{"name":"Zeitschrift fur Naturforschung. Section C, Biosciences","volume":"40 9-10","pages":"631-46"},"PeriodicalIF":0.0,"publicationDate":"1985-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1515/znc-1985-9-1008","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"15194567","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 32

Phosphorylation of coformycin and 2'-deoxycoformycin, and substrate and inhibitor properties of the nucleosides and nucleotides in several enzyme systems. coformycin和2'-脱氧coformycin的磷酸化，以及核苷和核苷酸在几种酶系统中的底物和抑制剂特性。

Zeitschrift fur Naturforschung. Section C, Biosciences Pub Date : 1985-09-01 DOI: 10.1515/znc-1985-9-1022

A Bzowska, P Lassota, D Shugar

{"title":"Phosphorylation of coformycin and 2'-deoxycoformycin, and substrate and inhibitor properties of the nucleosides and nucleotides in several enzyme systems.","authors":"A Bzowska, P Lassota, D Shugar","doi":"10.1515/znc-1985-9-1022","DOIUrl":"https://doi.org/10.1515/znc-1985-9-1022","url":null,"abstract":"Under conditions where 2'-deoxycoformycin is enzymatically phosphorylated by wheat shoot phosphotransferase to the 5'-phosphate in 15-20% yield, coformycin is a relatively poor substrate, and is phosphorylated only to the extent of less than or equal to 5%. However, chemical phosphorylation of coformycin by modifications of the Yoshikawa procedure led to isolation of coformycin-5'-phosphate in 20% overall yield. Coformycin-5'-phosphate was characterized by various criteria, including 1H NMR spectroscopy. Comparison of the spectrum with that of the parent nucleoside indicated that the nucleotide is predominantly, although not exclusively, in the conformation anti about the glycosidic bond. Like 2'-deoxycoformycin-5'-phosphate, coformycin-5'-phosphate was a feeble substrate of snake venom 5'-nucleotidase, and is hydrolyzed, quantitatively, at only 2% the rate for 5'-AMP. With 5'-AMP analogues as substrate, the 5'-phosphates of both coformycin and deoxycoformycin were poor inhibitors of the enzyme, with Ki values greater than 0.3 mM. The 5'-phosphates of both coformycin and deoxycoformycin do not significantly inhibit adenosine deaminase (Ki greater than 0.2 mM), but are potent inhibitors of adenylate deaminase (Ki less than or equal to 10(-9) M). Neither coformycin nor deoxycoformycin are inhibitors of mammalian purine nucleoside phosphorylase. The stabilities of coformycin, deoxycoformycin, and their 5'-phosphates, have been examined as a function of pH, and nature of the buffer medium. In particular, all exhibit instability in acid and neutral media, but are relatively stable in the vicinity of pH 9. Some biological aspects of the overall results are presented.","PeriodicalId":23914,"journal":{"name":"Zeitschrift fur Naturforschung. Section C, Biosciences","volume":"40 9-10","pages":"710-4"},"PeriodicalIF":0.0,"publicationDate":"1985-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1515/znc-1985-9-1022","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14135007","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5

Effects of norfloxacin and rifampicin on growth and streptolysin S production in hemolytic streptococci. 诺氟沙星和利福平对溶血性链球菌生长和产链溶素S的影响。

Zeitschrift fur Naturforschung. Section C, Biosciences Pub Date : 1985-09-01 DOI: 10.1515/znc-1985-9-1009

A Taketo, Y Taketo

引用次数: 0

Stereospecific synthesis by the sodium salt glycosylation method of halogeno benzimidazole 2'-deoxyribose analogues of the inhibitor of hnRNA synthesis, 5,6-dichloro-1-(beta-D-ribofuranosyl)benzimidazole (DRB). 用钠盐糖基化法立体定向合成卤代苯并咪唑2′-脱氧核糖类似物的hnRNA合成抑制剂5,6-二氯-1-(β -d -核呋喃基)苯并咪唑(DRB)。

Zeitschrift fur Naturforschung. Section C, Biosciences Pub Date : 1985-09-01 DOI: 10.1515/znc-1985-9-1023

Z Kazimierczuk, R Stolarski, D Shugar

引用次数: 8

Acetohydroxyacid synthase inhibitors: N-phthalyl-L-valine anilide and related compounds. 乙酰羟基酸合成酶抑制剂:n -邻苯酞- l-缬氨酸苯胺及相关化合物。

Zeitschrift fur Naturforschung. Section C, Biosciences Pub Date : 1985-09-01 DOI: 10.1515/znc-1985-9-1010

J L Huppatz, J E Casida

引用次数: 11

Regioselective O-demethylation of scoparone: differentiation between rat liver cytochrome P-450 isozymes. 天scopone的区域选择性o -去甲基化:大鼠肝细胞色素P-450同工酶的分化。

Zeitschrift fur Naturforschung. Section C, Biosciences Pub Date : 1985-09-01 DOI: 10.1515/znc-1985-9-1017

D Müller-Enoch, E Büttgen, A Nonnenmacher

引用次数: 9

The action of Saraca asoca Roxb. de Wilde bark on the PGH2 synthetase enzyme complex of the sheep vesicular gland. Saraca asoca Roxb的行动。绵羊囊腺PGH2合成酶复合物的研究。

Zeitschrift fur Naturforschung. Section C, Biosciences Pub Date : 1985-07-01 DOI: 10.1515/znc-1985-7-812

T B Middelkoop, R P Labadie

引用次数: 19