Zeitschrift fur Naturforschung. Section C, Biosciences最新文献_第3页

Composition and distribution of lipids in tissues of bogue (Boops boops). 蛙类(Boops Boops)组织中脂质的组成和分布。

Zeitschrift fur Naturforschung. Section C, Biosciences Pub Date : 1985-07-01 DOI: 10.1515/znc-1985-7-819

V M Kapoulas, S Miniadis-Meimaroglou

引用次数: 7

X-ray studies on phospholipid bilayers. V. Interactions with DDT. 磷脂双分子层的x射线研究。V.与滴滴涕的相互作用。

Zeitschrift fur Naturforschung. Section C, Biosciences Pub Date : 1985-07-01 DOI: 10.1515/znc-1985-7-820

M Suwalsky, N Bugueño, J Tapia, F Neira

引用次数: 8

Invariance of stoichiometry of the sarcoplasmic reticulum calcium pump at physiological calcium concentrations--a reevaluation. 生理钙浓度下肌浆网钙泵化学计量的不变性——重新评价。

Zeitschrift fur Naturforschung. Section C, Biosciences Pub Date : 1985-07-01 DOI: 10.1515/znc-1985-7-821

W Hasselbach, A Migala

引用次数: 3

Biosynthesis of trichothecene mycotoxins in Fusarium culmorum cultures. 镰刀菌培养中毛霉毒素的生物合成。

Zeitschrift fur Naturforschung. Section C, Biosciences Pub Date : 1985-07-01 DOI: 10.1515/znc-1985-7-810

N C Baldwin, B W Bycroft, P M Dewick, J Gilbert, I Holden

引用次数: 8

Isotopic effect on the kinetic of thermal denaturation of ceruloplasmin. 同位素对铜蓝蛋白热变性动力学的影响。

Zeitschrift fur Naturforschung. Section C, Biosciences Pub Date : 1985-07-01 DOI: 10.1515/znc-1985-7-817

L Sportelli, A Desideri, A Campaniello

引用次数: 5

[Comparison of various immune surface labeling methods for scanning electron microscopy with the example of a surface antigen protein of the yeast Candida albicans]. [以白色念珠菌表面抗原蛋白为例，比较扫描电镜下各种免疫表面标记方法]。

Zeitschrift fur Naturforschung. Section C, Biosciences Pub Date : 1985-07-01

M Borg

{"title":"[Comparison of various immune surface labeling methods for scanning electron microscopy with the example of a surface antigen protein of the yeast Candida albicans].","authors":"M Borg","doi":"","DOIUrl":"","url":null,"abstract":"The labeling of immunocomplexes for scanning electron microscopy (SEM) is a fairly new technique, and the various procedures, that have been proposed, have not yet been compared. Such comparative evaluation was performed with Candida protease as a target antigen. This secretory enzyme of the opportunistic yeast Candida albicans can be localized on the surface of fungal blastopores and mycelia, both after growth in proteinaceous medium and upon infection of murine peritoneal macrophages. The presence of the protease antigen was confirmed by immunofluorescence and by immunoperoxidase-light microscopy. The decoration of protease - anti protease complexes for SEM was attempted with colloids derived from the immunoperoxidase reaction, by the immunogold technique, and by antibodies linked to beads of synthetic polymers (polystyrene, polymethacrylate, polyacrolein). In addition, inactivated Staphylococcus aureus was used, which binds to antibodies through its protein-A. The high resolution by SEM of surface structures was matched only by the colloid based decoration techniques. All conjugates with beads suffered from inconsistent binding, which did not correspond with the distribution of the surface antigen. The comparatively best result with beads was obtained with polystyrene (Latex). Colloid based techniques in addition allow for critical point drying, which cannot be applied to synthetic beads in the usual manner.","PeriodicalId":23914,"journal":{"name":"Zeitschrift fur Naturforschung. Section C, Biosciences","volume":"40 7-8","pages":"539-50"},"PeriodicalIF":0.0,"publicationDate":"1985-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"15019359","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Prosomes are involved in the repression of viral mRNA. 蛋白体参与病毒mRNA的抑制。

Zeitschrift fur Naturforschung. Section C, Biosciences Pub Date : 1985-05-01 DOI: 10.1515/znc-1985-5-627

A Horsch, K Köhler, H P Schmid

引用次数: 9

Proton uptake by light induced interaction between rhodopsin and G-protein. 光诱导紫红质与g蛋白相互作用的质子摄取。

Zeitschrift fur Naturforschung. Section C, Biosciences Pub Date : 1985-05-01 DOI: 10.1515/znc-1985-5-619

A Schleicher, K P Hofmann

引用次数: 13

Localization of chromenes and benzofurans in the genus Encelia (Asteraceae). 菊科蕨属中铬烯类和苯并呋喃类的定位。

Zeitschrift fur Naturforschung. Section C, Biosciences Pub Date : 1985-05-01 DOI: 10.1515/znc-1985-5-602

P Proksch, M Proksch, W Weck, E Rodriguez

引用次数: 5

A small-angle X-ray scattering study on pre-irradiated malate synthase. The influence of formate, superoxide dismutase, and catalase on the X-ray induced aggregation of the enzyme. 预辐照苹果酸合酶的小角x射线散射研究。甲酸酯、超氧化物歧化酶和过氧化氢酶对x射线诱导的酶聚集的影响。

Zeitschrift fur Naturforschung. Section C, Biosciences Pub Date : 1985-05-01 DOI: 10.1515/znc-1985-5-614

P Zipper, R Wilfing, M Kriechbaum, H Durchschlag

{"title":"A small-angle X-ray scattering study on pre-irradiated malate synthase. The influence of formate, superoxide dismutase, and catalase on the X-ray induced aggregation of the enzyme.","authors":"P Zipper, R Wilfing, M Kriechbaum, H Durchschlag","doi":"10.1515/znc-1985-5-614","DOIUrl":"https://doi.org/10.1515/znc-1985-5-614","url":null,"abstract":"The sulfhydryl enzyme malate synthase from baker's yeast was X-irradiated with 6 kGy in air-saturated aqueous solution (enzyme concentration: congruent to 10 mg/ml; volume: 120 microliters), in the absence or presence of the specific scavengers formate, superoxide dismutase, and catalase. After X-irradiation, a small aliquot of the irradiated solutions was tested for enzymic activity while the main portion was investigated by means of small-angle X-ray scattering. Additionally, an unirradiated sample without additives was investigated as a reference. Experiments yielded the following results: X-irradiation in the absence of the mentioned scavengers caused considerable aggregation, fragmentation, and inactivation of the enzyme. The dose Dt37 for total (= repairable + non-repairable) inactivation resulted as 4.4 kGy. The mean radius of gyration was found to be about 13 nm. The mean degree of aggregation was obtained as 5.7, without correction for fragmentation. An estimation based on the thickness factor revealed that about 19% of material might be strongly fragmented. When this amount of fragments was accordingly taken into account, a value of 7.1 was obtained as an upper limit for the mean degree of aggregation. The observed retention of the thickness factor and the finding of two different cross-section factors are in full accord with the two-dimensional aggregation model established previously (Zipper and Durchschlag, Radiat. Environ. Biophys. 18, 99-121 (1980)). The presence of catalytic amounts of superoxide dismutase and/or catalase, in the absence of formate, during X-irradiation reduced both aggregation and inactivation significantly. The presence of formate (10 or 100 mM) during X-irradiation led to a strong decrease of aggregation and inactivation.(ABSTRACT TRUNCATED AT 250 WORDS)","PeriodicalId":23914,"journal":{"name":"Zeitschrift fur Naturforschung. Section C, Biosciences","volume":"40 5-6","pages":"364-72"},"PeriodicalIF":0.0,"publicationDate":"1985-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1515/znc-1985-5-614","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"15137312","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 6