生理钙浓度下肌浆网钙泵化学计量的不变性——重新评价。

W Hasselbach, A Migala
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引用次数: 3

摘要

最近的一项研究(a . Gafni和P. D. Boyer, Proc. Natl)观察到肌浆钙泵运输比率的下降。学会科学。(USA 82, 89-101[1985])是由于低钙/蛋白比时草酸钙沉淀的延迟。在净钙摄取过程中,普遍存在的高内部钙水平支持快速的钙回流和代偿性ATP水解,从而降低了运输比。然而,测定的钙回流并不能完全解释输运比的下降。外界钙(0.1微米)对泵的化学计量的假设调制与先前的研究结果不同,这些研究显示在相同的钙浓度范围内恒定的输运比为2。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Invariance of stoichiometry of the sarcoplasmic reticulum calcium pump at physiological calcium concentrations--a reevaluation.

The decline of the transport ratio of the sarcoplasmic calcium pump observed in a recent study (A. Gafni and P. D. Boyer, Proc. Natl. Acad. Sci. USA 82, 89-101 [1985] ) results from the retardation of calcium oxalate precipitation at low calcium/protein ratios. The prevailing high internal calcium level supports a rapid calcium backflux and a compensatory ATP hydrolysis during net calcium uptake which reduces the transport ratio. Yet, the determined calcium backflux does not fully account for the decline of the transport ratio. A supposed modulation of the stoichiometry of the pump by external calcium (0.1 microM) is at variance with results of previous studies showing a constant transport ratio of two in the same calcium concentration range.

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