Zeitschrift fur Naturforschung. Section C, Biosciences最新文献_第4页

Isolation of labeled lipoprotein from Escherichia coli and Proteus mirabilis after incubation with [14C]penicillin. [14C]青霉素培养大肠杆菌和神奇变形杆菌中标记脂蛋白的分离。

Zeitschrift fur Naturforschung. Section C, Biosciences Pub Date : 1985-05-01 DOI: 10.1515/znc-1985-5-622

G Gruner, M H Tadros, R Plapp

引用次数: 1

On the role of branched-chain amino acids in protein turnover of skeletal muscle. Studies in vivo with L-norleucine. 支链氨基酸在骨骼肌蛋白质周转中的作用。l -去甲亮氨酸的体内研究。

Zeitschrift fur Naturforschung. Section C, Biosciences Pub Date : 1985-05-01 DOI: 10.1515/znc-1985-5-624

K J Schott, J Gehrmann, U Pötter, V Neuhoff

{"title":"On the role of branched-chain amino acids in protein turnover of skeletal muscle. Studies in vivo with L-norleucine.","authors":"K J Schott, J Gehrmann, U Pötter, V Neuhoff","doi":"10.1515/znc-1985-5-624","DOIUrl":"https://doi.org/10.1515/znc-1985-5-624","url":null,"abstract":"The effect of L-norleucine, an isomer of leucine, on protein metabolism in vivo was studied in suckling rats. Rats were injected subcutaneously with various doses of L-norleucine (0.5 and 5.0 mumol/g body wt.) every 12 h from 3 to 15 days post partum. Protein concentration, amino acid concentrations, and incorporation of [3H]tyrosine into protein were analyzed in liver, muscles of thigh and small intestine. Amino acid concentrations and insulin levels in serum were also measured. At 5 days of age, norleucine induced an increase in protein concentration of skeletal muscle with an increased incorporation of [3H]tyrosine into protein indicating an accelerated protein synthesis. Changes in protein metabolism were paralleled by alterations in the amino acid pattern of this tissue. When protein concentration and protein synthesis were increased in skeletal muscle, protein concentration of small intestine was decreased, accompanied by elevated levels of amino acids in tissue. Protein synthesis of small intestine was not altered by the norleucine treatment. The results suggest a close interrelationship between skeletal muscle and small intestine with respect to protein turnover. The effects of norleucine were less pronounced at 10 and 15 days of age, which indicates a metabolic adaptation to the treatment. Alterations in amino acid concentrations of tissue due to changes in protein metabolism were not uniform but tissue-specific. Current concepts for explaining the effects of branched-chain amino acids (BCAA) on protein turnover in skeletal muscle are based on the assumption that the BCAA or leucine alone might become rate-limiting for protein synthesis in muscle under catabolic conditions. The amino acid analogue norleucine, however, cannot replace any of the BCAA in protein. Additionally, norleucine affected protein metabolism in highly anabolic organisms. Therefore, the present thoughts on this issue appear to be incomplete.","PeriodicalId":23914,"journal":{"name":"Zeitschrift fur Naturforschung. Section C, Biosciences","volume":"40 5-6","pages":"427-37"},"PeriodicalIF":0.0,"publicationDate":"1985-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1515/znc-1985-5-624","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"15009544","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Biochemical oxygen activation as the basis for the physiological action of tetrachlorodecaoxide (TCDO). 生化氧活化是四氯十氧化物(TCDO)生理作用的基础。

Zeitschrift fur Naturforschung. Section C, Biosciences Pub Date : 1985-05-01 DOI: 10.1515/znc-1985-5-621

R J Youngman, G R Wagner, F W Kühne, E F Elstner

引用次数: 16

Acyl chain specificity and kinetic properties of phospholipase A1 and A2 of bone marrow-derived macrophages. 骨髓源性巨噬细胞磷脂酶A1和A2的酰基链特异性和动力学性质。

Zeitschrift fur Naturforschung. Section C, Biosciences Pub Date : 1985-05-01 DOI: 10.1515/znc-1985-5-613

I Flesch, B Schmidt, E Ferber

{"title":"Acyl chain specificity and kinetic properties of phospholipase A1 and A2 of bone marrow-derived macrophages.","authors":"I Flesch, B Schmidt, E Ferber","doi":"10.1515/znc-1985-5-613","DOIUrl":"https://doi.org/10.1515/znc-1985-5-613","url":null,"abstract":"The fatty acyl specificity of phospholipase A1 and A2 in homogenates of mouse bone marrow-derived macrophages was determined using phosphatidylcholine and phosphatidylethanolamine of different acyl chain composition. Phosphatidylcholine with arachidonoyl at position 2 was cleaved preferentially by an alkaline phospholipase A2 (pH-optimum 9.0) leading to selective liberation of arachidonic acid. In contrast, phosphatidylcholines with oleoyl or linoleoyl at position 2 were degraded mainly by an acid phospholipase A1 (pH-optimum 4-5) resulting in a conservation of these fatty acids esterified in lysophosphatides. Substrate kinetics of the alkaline phospholipase A2 revealed a 30 fold higher affinity (Km = 3.8 X 10(-7) M) for 1-acyl-2-arachidonoyl-glycerophosphocholine compared to 1-acyl-2-oleoyl-glycerophosphocholine. The kinetic data were not influenced by endogenous lipids indicating that exogenous substrates do not equilibrate with cellular lipids. These results are suitable to explain a selective liberation of arachidonic acid from a mixture of phospholipids.","PeriodicalId":23914,"journal":{"name":"Zeitschrift fur Naturforschung. Section C, Biosciences","volume":"40 5-6","pages":"356-63"},"PeriodicalIF":0.0,"publicationDate":"1985-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1515/znc-1985-5-613","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"15137318","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 19

Chemiluminescence in the coupled oxidation of lecithin and ascorbate. 卵磷脂和抗坏血酸偶联氧化中的化学发光。

Zeitschrift fur Naturforschung. Section C, Biosciences Pub Date : 1985-03-01 DOI: 10.1515/znc-1985-3-415

K Lichszteld, Z Machoy, A Stepińska

引用次数: 0

Rate and rate-determining step of hydrogen-atom-induced strand breakage in poly(U) in aqueous solution under anoxic conditions. 缺氧条件下水溶液中聚(U)中氢原子诱导链断裂的速率及速率决定步骤。

Zeitschrift fur Naturforschung. Section C, Biosciences Pub Date : 1985-03-01 DOI: 10.1515/znc-1985-3-419

E Bothe, H Selbach

引用次数: 14

Enhanced production of cell-bound and extracellular streptolysin S by hemolytic streptococci pretreated with proteases. 经蛋白酶预处理的溶血性链球菌增强细胞结合和细胞外溶血素S的产生。

Zeitschrift fur Naturforschung. Section C, Biosciences Pub Date : 1985-03-01 DOI: 10.1515/znc-1985-3-404

A Taketo, Y Taketo

引用次数: 1

Site of H atom attack on uracil and its derivatives in aqueous solution. 水溶液中氢原子攻击尿嘧啶及其衍生物的位置。

Zeitschrift fur Naturforschung. Section C, Biosciences Pub Date : 1985-03-01 DOI: 10.1515/znc-1985-3-425

S Das, D J Deeble, C von Sonntag

引用次数: 21

Nucleotide sequence of bovine 1.723 satellite DNA. 牛1.723卫星DNA的核苷酸序列。

Zeitschrift fur Naturforschung. Section C, Biosciences Pub Date : 1985-03-01 DOI: 10.1515/znc-1985-3-418

G Plucienniczak, J Skowronski, A Plucienniczak, J Jaworski

引用次数: 2

Properties of lectins from snails of the genus Helix probed by monoclonal antibodies. 单克隆抗体检测螺旋属蜗牛凝集素的性质。

Zeitschrift fur Naturforschung. Section C, Biosciences Pub Date : 1985-03-01

H A Schneider

{"title":"Properties of lectins from snails of the genus Helix probed by monoclonal antibodies.","authors":"H A Schneider","doi":"","DOIUrl":"","url":null,"abstract":"Monoclonal antibodies were raised against the lectin of Helix pomatia (HPL). Besides antibodies bearing the more common gamma and kappa chains, antibodies with alpha, mu and lambda 2 chains were elicited. The anti-HPL antibodies are expected to be useful in studies on HPL biogenesis and HPL substructure and in studies concerned with the binding of HPL to cell surfaces. Binding of carbohydrates to HPL impaired the binding of anti-HPL antibodies. One to 3 mM GalNAc inhibited HPL-binding in two out of nine antibodies. None of the antibodies bound in the presence of micrograms per ml of the polyvalent blood group A-substance from hog stomach. Similarly, all anti-HPL antibodies were prevented from binding if non-inhibitory concentrations of A-substance were supplemented with GalNAc. Lectins from Helix aspersa (HAL) and Helix lucorum (HLL) differed from HPL in antigenic properties. Only one anti-HPL antibody each bound these lectins as well as HPL. Binding of lectins of Cepaea and Rapana was scarcely detectable. Most of the anti-HPL antibodies and the multivalent HPL-antigens formed precipitation lines in double diffusion tests. At least two antibodies (IgMs) did so with HLL but none with HAL. The possibility that antibodies were selected because of unknown interactions between HPL and the carbohydrate moieties of certain fractions of antibodies was excluded by raising the antibodies in the presence of tunicamycin to inhibit N-glycosylation.","PeriodicalId":23914,"journal":{"name":"Zeitschrift fur Naturforschung. Section C, Biosciences","volume":"40 3-4","pages":"254-61"},"PeriodicalIF":0.0,"publicationDate":"1985-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"15039238","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0