Stimulation of brain synaptosome-associated adenylate cyclase by acidic phospholipids.

S Tsakiris
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引用次数: 1

Abstract

Phosphatidylserine (PS), phosphatidylinositol (PIN) or phosphatidylglycerol (PGL) incubated with synaptosomal plasma membranes (SPM) of dog brain, stimulated adenylate cyclase. The enzyme activity showed a dramatic increase at around 1.6 mumol PS/mg protein, while use of higher concentrations led to inhibition of the activity with respect to the maximal percentage of stimulation. Moreover, PS stimulated the dopamine-sensitive adenylate cyclase. Solubilization of SPM by the detergent Lubrol-PX did not affect the enzyme activation induced by dopamine. The solubilization, also, showed that the enzyme activity does not change at any PS, PIN or PGL concentration used. These results indicate that acidic phospholipids do not directly act on adenylate cyclase, but indirectly, affecting the membrane fluidity probably. Such modifications of interactions through lipid-protein(s) of adenylate cyclase may have implications to physiological responses to hormones or/and neurotransmitters in the central nervous system.

酸性磷脂对脑突触体相关腺苷酸环化酶的刺激。
磷脂酰丝氨酸(PS)、磷脂酰肌醇(PIN)或磷脂酰甘油(PGL)与犬脑突触体质膜(SPM)孵育,刺激腺苷酸环化酶。在1.6 mumol PS/mg蛋白附近,酶活性显著增加,而使用较高的浓度导致活性受到最大刺激百分比的抑制。此外,PS刺激多巴胺敏感腺苷酸环化酶。洗涤剂Lubrol-PX对SPM的增溶作用不影响多巴胺诱导的酶活化。溶解实验也表明,酶活性在任何PS、PIN或PGL浓度下都没有变化。这些结果表明,酸性磷脂不直接作用于腺苷酸环化酶,而可能间接影响膜的流动性。这种通过脂质-蛋白腺苷酸环化酶相互作用的改变可能与中枢神经系统对激素或/和神经递质的生理反应有关。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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