Yeast最新文献_第6页

High nitrogen concentration causes G2/M arrest in Hanseniaspora vineae. 高氮浓度引起海参G2/M阻滞。

IF 2.6 4区生物学

Yeast Pub Date : 2023-12-01 Epub Date: 2023-11-23 DOI: 10.1002/yea.3911

Luisa Vivian Schwarz, Fernanda Knaach Sandri, Fernando Scariot, Ana Paula Longaray Delamare, Maria Jose Valera, Francisco Carrau, Sergio Echeverrigaray

{"title":"High nitrogen concentration causes G2/M arrest in Hanseniaspora vineae.","authors":"Luisa Vivian Schwarz, Fernanda Knaach Sandri, Fernando Scariot, Ana Paula Longaray Delamare, Maria Jose Valera, Francisco Carrau, Sergio Echeverrigaray","doi":"10.1002/yea.3911","DOIUrl":"10.1002/yea.3911","url":null,"abstract":"Yeasts have been widely used as a model to better understand cell cycle mechanisms and how nutritional and genetic factors can impact cell cycle progression. While nitrogen scarcity is well known to modulate cell cycle progression, the relevance of nitrogen excess for microorganisms has been overlooked. In our previous work, we observed an absence of proper entry into the quiescent state in Hanseniaspora vineae and identified a potential link between this behavior and nitrogen availability. Furthermore, the Hanseniaspora genus has gained attention due to a significant loss of genes associated with DNA repair and cell cycle. Thus, the aim of our study was to investigate the effects of varying nitrogen concentrations on H. vineae's cell cycle progression. Our findings demonstrated that nitrogen excess, regardless of the source, disrupts cell cycle progression and induces G2/M arrest in H. vineae after reaching the stationary phase. Additionally, we observed a viability decline in H. vineae cells in an ammonium-dependent manner, accompanied by increased production of reactive oxygen species, mitochondrial hyperpolarization, intracellular acidification, and DNA fragmentation. Overall, our study highlights the events of the cell cycle arrest in H. vineae induced by nitrogen excess and attempts to elucidate the possible mechanism triggering this absence of proper entry into the quiescent state.","PeriodicalId":23870,"journal":{"name":"Yeast","volume":null,"pages":null},"PeriodicalIF":2.6,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138300149","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Taxogenomic analysis of a novel yeast species isolated from soil, Pichia galeolata sp. nov. 从土壤中分离的一种新酵母Pichia galeolata sp.nov.的分类基因组分析。

IF 2.6 4区生物学

Yeast Pub Date : 2023-12-01 Epub Date: 2023-11-03 DOI: 10.1002/yea.3905

Dana A Opulente, Quinn K Langdon, Martin Jarzyna, Kelly V Buh, Max A B Haase, Marizeth Groenewald, Chris Todd Hittinger

引用次数: 0

Strain diversity in Saccharomyces cerevisiae thiamine production capacity. 酿酒酵母硫胺素生产能力的菌株多样性。

IF 2.6 4区生物学

Yeast Pub Date : 2023-12-01 Epub Date: 2023-11-06 DOI: 10.1002/yea.3906

Rebecca Rocchi, Judith C M Wolkers-Rooijackers, Zhuotong Liao, Marcel H Tempelaars, Eddy J Smid

{"title":"Strain diversity in Saccharomyces cerevisiae thiamine production capacity.","authors":"Rebecca Rocchi, Judith C M Wolkers-Rooijackers, Zhuotong Liao, Marcel H Tempelaars, Eddy J Smid","doi":"10.1002/yea.3906","DOIUrl":"10.1002/yea.3906","url":null,"abstract":"Vitamin B1 , also known as thiamine, is an important vitamin that, besides its role in human health, is converted to meat aromas upon exposure to high temperatures. Therefore, it is relevant for the production of vegan meat-like flavours. In this study, we investigated 48 Saccharomyces cerevisiae strains for their thiamine production capacity by measuring the intracellular and extracellular vitamins produced in the thiamine-free minimal medium after 72 h of growth. We found approximately an 8.2-fold difference in overall thiamine yield between the highest and lowest-producing strains. While the highest thiamine yield was 254.6 nmol/L, the highest thiamine-specific productivity was 160.9 nmol/g DW. To assess whether extracellular thiamine was due to leakage caused by cell damage, we monitored membrane permeabilization using propidium iodide (PI) staining and flow cytometry. We found a good correlation between the percentage of extracellular thiamine and PI-stained cells (Spearman's ρ = 0.85). Finally, we compared S. cerevisiae CEN.PK113-7D (wild type [WT]) to three strains evolved in a thiamine-free medium for their thiamine production capacity. On average, we saw an increase in the amount of thiamine produced. One of the evolved strains had a 49% increase in intracellular thiamine-specific productivity and a biomass increase of 20% compared with the WT. This led to a total increase in thiamine yield of 60% in this strain, reaching 208 nmol/L. This study demonstrated that it is possible to achieve thiamine overproduction in S. cerevisiae via strain selection and adaptive laboratory evolution.","PeriodicalId":23870,"journal":{"name":"Yeast","volume":null,"pages":null},"PeriodicalIF":2.6,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71486677","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Yueomyces silvicola sp. nov., a novel ascomycetous yeast species unable to utilize ammonium, glutamate, and glutamine as sole nitrogen sources. silvicola Yuomyces sp.nov.，一种新的子囊菌酵母，不能利用铵、谷氨酸和谷氨酰胺作为唯一的氮源。

IF 2.6 4区生物学

Yeast Pub Date : 2023-11-01 Epub Date: 2023-10-11 DOI: 10.1002/yea.3901

Hong-Tao Yu, Yu-Jie Shang, Hai-Yan Zhu, Pei-Jie Han, Qi-Ming Wang, Ana Raquel O Santos, Katharina O Barros, Gisele F L Souza, Flávia B M Alvarenga, Maxwel A Abegg, Carlos A Rosa, Feng-Yan Bai

{"title":"Yueomyces silvicola sp. nov., a novel ascomycetous yeast species unable to utilize ammonium, glutamate, and glutamine as sole nitrogen sources.","authors":"Hong-Tao Yu, Yu-Jie Shang, Hai-Yan Zhu, Pei-Jie Han, Qi-Ming Wang, Ana Raquel O Santos, Katharina O Barros, Gisele F L Souza, Flávia B M Alvarenga, Maxwel A Abegg, Carlos A Rosa, Feng-Yan Bai","doi":"10.1002/yea.3901","DOIUrl":"10.1002/yea.3901","url":null,"abstract":"Five yeast strains isolated from tree bark and rotten wood collected in central and southwestern China, together with four Brazilian strains (three from soil and rotting wood collected in an Amazonian rainforest biome and one from Bromeliad collected in Alagoas state) and one Costa Rican strain isolated from a flower beetle, represent a new species closely related with Yueomyces sinensis in Saccharomycetaceae, as revealed by the 26S ribosomal RNA gene D1/D2 domain and the internal transcribed spacer region sequence analysis. The name Yueomyces silvicola sp. nov. is proposed for this new species with the holotype China General Microbiological Culture Collection Center 2.6469 (= Japan Collection of Microorganisms 34885). The new species exhibits a whole-genome average nucleotide identity value of 77.8% with Y. sinensis. The two Yueomyces species shared unique physiological characteristics of being unable to utilize ammonium and the majority of the amino acids, including glutamate and glutamine, as sole nitrogen sources. Among the 20 amino acids tested, only leucine and tyrosine can be utilized by the Yueomyces species. Genome sequence comparison showed that GAT1, which encodes a GATA family protein participating in transcriptional activation of nitrogen-catabolic genes in Saccharomyces cerevisiae, is absent in the Yueomyces species. However, the failure of the Yueomyces species to utilize ammonium, glutamate, and glutamine, which are generally preferred nitrogen sources for microorganisms, implies that more complicated alterations in the central nitrogen metabolism pathway might occur in the genus Yueomyces.","PeriodicalId":23870,"journal":{"name":"Yeast","volume":null,"pages":null},"PeriodicalIF":2.6,"publicationDate":"2023-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41214477","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Unilateral ends-out gene targeting increases mistargeting through supporting extensive single-strand assimilation. 单向终止基因靶向通过支持广泛的单链同化增加了错误靶向。

IF 2.6 4区生物学

Yeast Pub Date : 2023-11-01 Epub Date: 2023-10-08 DOI: 10.1002/yea.3899

Petar Tomev Mitrikeski

{"title":"Unilateral ends-out gene targeting increases mistargeting through supporting extensive single-strand assimilation.","authors":"Petar Tomev Mitrikeski","doi":"10.1002/yea.3899","DOIUrl":"10.1002/yea.3899","url":null,"abstract":"Ends-out gene targeting enables the swapping of endogenous alleles with exogenous ones through homologous recombination which bears great implications both fundamental and applicable. To address the recombination mechanism(s) behind it, an experimental system was designed to distinguish between a possible (but rarely active) unilateral and the expected bilateral targeting in the yeast Saccharomyces cerevisiae in which the proportions of the two alternative genetic outcomes are conceived to mirror the probabilities of the two scenarios. The quantitative analysis showed that the bilateral targeting was expectedly predominant. However, an analogous comparative analysis on a different experimental set suggested a prevalence of unilateral targeting unveiling an uncertainty whether the extensively resected targeting modules only mimic unilateral invasion. Based on this, a comprehensive qualitative analysis was conducted revealing a single basic ends-out gene targeting mechanism composed of two intertwined pathways differing in the way how the homologous invasion is initiated and/or the production of the intermediates is conducted. This study suggests that bilateral targeting lowers mistargeting plausibly by limiting strand assimilation, unlike unilateral targeting which may initiate extensive strand assimilation producing intermediates capable of supporting multiple genetic outcomes which leads to mistargeting. Some of these outcomes can also be produced by mimicking unilateral invasion.","PeriodicalId":23870,"journal":{"name":"Yeast","volume":null,"pages":null},"PeriodicalIF":2.6,"publicationDate":"2023-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41116741","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Efficient PCR-based gene targeting in isolates of the nonconventional yeast Debaryomyces hansenii. 基于PCR的高效基因靶向非常规酵母汉氏双核菌分离株。

IF 2.6 4区生物学

Yeast Pub Date : 2023-11-01 Epub Date: 2023-10-23 DOI: 10.1002/yea.3902

Sondos Alhajouj, Selva Turkolmez, Tarad Abalkhail, Zeena Hadi Obaid Alwan, Daniel James Gilmour, Phil J Mitchell, Ewald H Hettema

引用次数: 1

Yeasts from tropical forests: Biodiversity, ecological interactions, and as sources of bioinnovation. 热带森林的酵母：生物多样性，生态相互作用，以及作为生物创新的来源。

IF 2.6 4区生物学

Yeast Pub Date : 2023-11-01 Epub Date: 2023-11-03 DOI: 10.1002/yea.3903

Carlos A Rosa, Marc-André Lachance, Savitree Limtong, Ana R O Santos, Mellisa F Landell, Andreas K Gombert, Paula B Morais, José P Sampaio, Carla Gonçalves, Paula Gonçalves, Aristóteles Góes-Neto, Rosângela Santa-Brígida, Marlúcia B Martins, Daniel H Janzen, Winnie Hallwachs

{"title":"Yeasts from tropical forests: Biodiversity, ecological interactions, and as sources of bioinnovation.","authors":"Carlos A Rosa, Marc-André Lachance, Savitree Limtong, Ana R O Santos, Mellisa F Landell, Andreas K Gombert, Paula B Morais, José P Sampaio, Carla Gonçalves, Paula Gonçalves, Aristóteles Góes-Neto, Rosângela Santa-Brígida, Marlúcia B Martins, Daniel H Janzen, Winnie Hallwachs","doi":"10.1002/yea.3903","DOIUrl":"10.1002/yea.3903","url":null,"abstract":"Tropical rainforests and related biomes are found in Asia, Australia, Africa, Central and South America, Mexico, and many Pacific Islands. These biomes encompass less than 20% of Earth's terrestrial area, may contain about 50% of the planet's biodiversity, and are endangered regions vulnerable to deforestation. Tropical rainforests have a great diversity of substrates that can be colonized by yeasts. These unicellular fungi contribute to the recycling of organic matter, may serve as a food source for other organisms, or have ecological interactions that benefit or harm plants, animals, and other fungi. In this review, we summarize the most important studies of yeast biodiversity carried out in these biomes, as well as new data, and discuss the ecology of yeast genera frequently isolated from tropical forests and the potential of these microorganisms as a source of bioinnovation. We show that tropical forest biomes represent a tremendous source of new yeast species. Although many studies, most using culture-dependent methods, have already been carried out in Central America, South America, and Asia, the tropical forest biomes of Africa and Australasia remain an underexplored source of novel yeasts. We hope that this review will encourage new researchers to study yeasts in unexplored tropical forest habitats.","PeriodicalId":23870,"journal":{"name":"Yeast","volume":null,"pages":null},"PeriodicalIF":2.6,"publicationDate":"2023-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71427440","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Visioning synthetic futures for yeast research within the context of current global techno-political trends. 在当前全球技术政治趋势的背景下，展望酵母研究的合成未来。

IF 2.6 4区生物学

Yeast Pub Date : 2023-10-01 Epub Date: 2023-08-31 DOI: 10.1002/yea.3897

Thomas A Dixon, Roy S K Walker, Isak S Pretorius

{"title":"Visioning synthetic futures for yeast research within the context of current global techno-political trends.","authors":"Thomas A Dixon, Roy S K Walker, Isak S Pretorius","doi":"10.1002/yea.3897","DOIUrl":"10.1002/yea.3897","url":null,"abstract":"Yeast research is entering into a new period of scholarship, with new scientific tools, new questions to ask and new issues to consider. The politics of emerging and critical technology can no longer be separated from the pursuit of basic science in fields, such as synthetic biology and engineering biology. Given the intensifying race for technological leadership, yeast research is likely to attract significant investment from government, and that it offers huge opportunities to the curious minded from a basic research standpoint. This article provides an overview of new directions in yeast research with a focus on Saccharomyces cerevisiae, and places these trends in their geopolitical context. At the highest level, yeast research is situated within the ongoing convergence of the life sciences with the information sciences. This convergent effect is most strongly pronounced in areas of AI-enabled tools for the life sciences, and the creation of synthetic genomes, minimal genomes, pan-genomes, neochromosomes and metagenomes using computer-assisted design tools and methodologies. Synthetic yeast futures encompass basic and applied science questions that will be of intense interest to government and nongovernment funding sources. It is essential for the yeast research community to map and understand the context of their research to ensure their collaborations turn global challenges into research opportunities.","PeriodicalId":23870,"journal":{"name":"Yeast","volume":null,"pages":null},"PeriodicalIF":2.6,"publicationDate":"2023-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10502486","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Sequential inoculation of flocculent Torulaspora delbrueckii with Saccharomyces cerevisiae increases color density of Pinot Noir wines. 用酿酒酵母连续接种絮凝的德氏Torulaspora delbrueckii可提高黑比诺葡萄酒的色密度。

IF 2.6 4区生物学

Yeast Pub Date : 2023-10-01 Epub Date: 2023-08-31 DOI: 10.1002/yea.3896

Katasha S McCullough, Yi Yang, Melodie A Lindsay, Neill Culley, Rebecca C Deed

{"title":"Sequential inoculation of flocculent Torulaspora delbrueckii with Saccharomyces cerevisiae increases color density of Pinot Noir wines.","authors":"Katasha S McCullough, Yi Yang, Melodie A Lindsay, Neill Culley, Rebecca C Deed","doi":"10.1002/yea.3896","DOIUrl":"10.1002/yea.3896","url":null,"abstract":"Pinot noir grapes require careful management in the winery to prevent loss of color density and promote aging stability. Winemaking with flocculent yeast has been shown to increase color density, which is desirable to consumers. This research explored interspecies sequential inoculation and co-flocculation of commercial yeast on Pinot noir wine color. Sedimentation rates of six non-Saccharomyces species and two Saccharomyces cerevisiae strains were assayed individually and in combination. The most flocculent pairings, Torulaspora delbrueckii BIODIVA with S. cerevisiae RC212 or VL3, were used to ferment 20 L Pinot noir must. Sequential fermentations produced wines with greater color density at 420 + 520 nm, confirmed by sensory panel. Total and monomeric anthocyanin concentrations were decreased in sequentially fermented wines, despite being the main source of red wine color. BIODIVA adsorbed more anthocyanins than S. cerevisiae, indicating a greater number of cell wall mannoproteins in flocculent yeast, that could then result in a later release of anthocyanins and enhance copigment formation in red wines.","PeriodicalId":23870,"journal":{"name":"Yeast","volume":null,"pages":null},"PeriodicalIF":2.6,"publicationDate":"2023-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10122144","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Presence of Saccharomyces eubayanus in fermentative environments reveals a new adaptive scenario in Patagonia. 真巴亚努斯酵母在发酵环境中的存在揭示了巴塔哥尼亚的一种新的适应性场景。

IF 2.6 4区生物学

Yeast Pub Date : 2023-10-01 Epub Date: 2023-08-18 DOI: 10.1002/yea.3894

Melisa Gonzalez-Flores, Ana V Delfino, María E Rodríguez, Christian A Lopes

{"title":"Presence of Saccharomyces eubayanus in fermentative environments reveals a new adaptive scenario in Patagonia.","authors":"Melisa Gonzalez-Flores, Ana V Delfino, María E Rodríguez, Christian A Lopes","doi":"10.1002/yea.3894","DOIUrl":"10.1002/yea.3894","url":null,"abstract":"Patagonia (Argentina and Chile) harbors the highest Saccharomyces eubayanus genomic diversity and its widest predominance in natural environments. In this work, S. eubayanus was isolated for the first time from a fermentative environment. This species was found dominating both a traditional apple chicha fermentation as well as feral apple trees in the Andean region of Aluminé (Argentina). S. eubayanus was the only Saccharomyces species found in the isolation substrates, although it coexisted with other non-Saccharomyces species. The absence of strong fermentative competitors of the Saccharomyces genus (like Saccharomyces uvarum or Saccharomyces cerevisiae) in the feral apples could promote the development and implantation of S. eubayanus in a spontaneous apple must fermentation. Phylogeographic analyses revealed a high intraspecific diversity in S. eubayanus, enabling the characterization of strains belonging to the genomic subpopulations PA1, PA2, and PB1 according to the sequences obtained for the intFR gene region. This result evidence that the studied sampling area represents a natural habitat for the species. Being a novel finding, studying the causes that allowed this species to prosper in a fermentative environment becomes essential. Hence, the physiological profile of the new isolates, including their ability to grow at different temperature, nitrogen, and ethanol concentrations was evaluated in comparison with a set of S. eubayanus strains previously isolated from natural environment and representing different genomic subpopulations. Greater physiological diversity was evidenced when strains isolated from both natural and fermentative environments were analyzed overall. Furthermore, no direct relationship between genomic population and physiological behavior was observed; on the opposite, strains appeared to exhibit similar behavior, primarily grouped by isolation origin.","PeriodicalId":23870,"journal":{"name":"Yeast","volume":null,"pages":null},"PeriodicalIF":2.6,"publicationDate":"2023-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10024148","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0