IF 2.6 4区生物学

Yeast Pub Date : 2023-03-01 DOI: 10.1002/yea.3848

Mariana Natalino, Marco Fumasoni

{"title":"Experimental approaches to study evolutionary cell biology using yeasts.","authors":"Mariana Natalino, Marco Fumasoni","doi":"10.1002/yea.3848","DOIUrl":"https://doi.org/10.1002/yea.3848","url":null,"abstract":"The past century has witnessed tremendous advances in understanding how cells function. Nevertheless, how cellular processes have evolved is still poorly understood. Many studies have highlighted surprising molecular diversity in how cells from diverse species execute the same processes, and advances in comparative genomics are likely to reveal much more molecular diversity than was believed possible until recently. Extant cells remain therefore the product of an evolutionary history that we vastly ignore. Evolutionary cell biology has emerged as a discipline aiming to address this knowledge gap by combining evolutionary, molecular, and cellular biology thinking. Recent studies have shown how even essential molecular processes, such as DNA replication, can undergo fast adaptive evolution under certain laboratory conditions. These developments open new lines of research where the evolution of cellular processes can be investigated experimentally. Yeasts naturally find themselves at the forefront of this research line. Not only do they allow the observation of fast evolutionary adaptation, but they also provide numerous genomic, synthetic, and cellular biology tools already developed by a large community. Here we propose that yeasts can serve as an \"evolutionary cell lab\" to test hypotheses, principles, and ideas in evolutionary cell biology. We discuss various experimental approaches available for this purpose, and how biology at large can benefit from them.","PeriodicalId":23870,"journal":{"name":"Yeast","volume":"40 3-4","pages":"123-133"},"PeriodicalIF":2.6,"publicationDate":"2023-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10375262","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Emergence of [GAR⁺ ] cells in yeast from sake brewing affects the fermentation properties. 清酒酿造过程中酵母中[GAR+]细胞的出现影响了发酵性能。

IF 2.6 4区生物学

Yeast Pub Date : 2023-03-01 DOI: 10.1002/yea.3844

Koichi Tanabe, Natshumi Maeda, Honoka Okumura, Jun Shima

引用次数: 0

Insights from yeast: Transcriptional reprogramming following metformin treatment is similar to that of deferiprone in a yeast Friedreich's ataxia model. 酵母的见解:二甲双胍治疗后的转录重编程与酵母弗里德赖希共济失调模型中的去铁蛋白相似。

IF 2.6 4区生物学

Yeast Pub Date : 2023-03-01 DOI: 10.1002/yea.3845

Esra Börklü

{"title":"Insights from yeast: Transcriptional reprogramming following metformin treatment is similar to that of deferiprone in a yeast Friedreich's ataxia model.","authors":"Esra Börklü","doi":"10.1002/yea.3845","DOIUrl":"https://doi.org/10.1002/yea.3845","url":null,"abstract":"In the absence of YFH1, the yeast ortholog of the human FXN gene, budding yeast Saccharomyces cerevisiae experience similar problems to those of cells with Friedreich's ataxia (FRDA). The comparable phenotypic traits consist of impaired respiration, problems in iron homeostasis, decreased oxidative stress tolerance, and diminished iron-sulfur cluster synthesis, rendering yeast of potential use in FRDA modeling and drug trials. Deferiprone, an iron chelator, is one of the long-term studied potential drugs for FRDA, whereas metformin is a biguanide prescribed to treat type 2 diabetes. In the present study, the effects of deferiprone and metformin treatment on the yeast FRDA model are explored via RNA-sequencing analyses. The comparative inquiry of transcriptome data reveals new promising roles for metformin in FRDA treatment since deferiprone and metformin treatments produce overlapping transcriptional and phenotypic responses in YFH1Δ cells. The results revealed that both deferiprone and metformin treatment does not rescue aerobic respiration in YFH1Δ cells, but they alleviate the FRDA phenotype probably by triggering the retrograde mitochondria-to-nucleus signaling.","PeriodicalId":23870,"journal":{"name":"Yeast","volume":"40 3-4","pages":"143-151"},"PeriodicalIF":2.6,"publicationDate":"2023-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9320187","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ger1 is a secreted aspartic acid protease essential for spore germination in Ustilago maydis. Ger1是麦草黑穗菌孢子萌发所必需的一种分泌性天冬氨酸蛋白酶。

IF 2.6 4区生物学

Yeast Pub Date : 2023-02-01 DOI: 10.1002/yea.3835

Subhasish Mukherjee, Koustav Bhakta, Abhrajyoti Ghosh, Anupama Ghosh

{"title":"Ger1 is a secreted aspartic acid protease essential for spore germination in Ustilago maydis.","authors":"Subhasish Mukherjee, Koustav Bhakta, Abhrajyoti Ghosh, Anupama Ghosh","doi":"10.1002/yea.3835","DOIUrl":"https://doi.org/10.1002/yea.3835","url":null,"abstract":"Ustilago maydis expresses a number of proteases during its pathogenic lifecycle. Some of the proteases including both intracellular and extracellular ones have previously been shown to influence the virulence of the pathogen. However, any role of secreted proteases in the sporulation process of U. maydis have not been explored earlier. In this study we have investigated the biological function of one such secreted protease, Ger1 belonging to aspartic protease A1 family. An assessment of the real time expression of ger1 revealed an infection specific expression of the protein especially during late phases of infection. We also evaluated any contribution of the protein in the pathogenicity of the fungus. Our data revealed an involvement of Ger1 in the sporulation and spore germination processes of U. maydis. Ger1 also showed positive influence on the pathogenicity of the fungus and accordingly the ger1 deletion mutant exhibited reduced pathogenicity. The study also demonstrated the protease activity associated with Ger1 to be essential for its biological function. Fluorescence microscopy of maize plants infected with U. maydis cells expressing Ger1-mcherry-HA also revealed that Ger1 is efficiently secreted within maize apoplast.","PeriodicalId":23870,"journal":{"name":"Yeast","volume":"40 2","pages":"102-116"},"PeriodicalIF":2.6,"publicationDate":"2023-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9432669","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Serine metabolism contributes to cell survival by regulating extracellular pH and providing an energy source in Saccharomyces cerevisiae. 在酿酒酵母中，丝氨酸代谢通过调节细胞外pH值和提供能量来源来促进细胞存活。

IF 2.6 4区生物学

Yeast Pub Date : 2023-02-01 DOI: 10.1002/yea.3840

Arisa Kawamukai, Ayana Iwano, Momoka Shibata, Yuko Kishi, Akira Matsuura

引用次数: 1

Heterologous expression reveals unique properties of Trk K⁺ importers from nonconventional biotechnologically relevant yeast species together with their potential to support Saccharomyces cerevisiae growth. 异源表达揭示了来自非传统生物技术相关酵母菌种的Trk +进口商的独特特性，以及它们支持酿酒酵母生长的潜力。

IF 2.6 4区生物学

Yeast Pub Date : 2023-02-01 DOI: 10.1002/yea.3834

Klára Papoušková, Marcos Gómez, Marie Kodedová, José Ramos, Olga Zimmermannová, Hana Sychrová

{"title":"Heterologous expression reveals unique properties of Trk K+ importers from nonconventional biotechnologically relevant yeast species together with their potential to support Saccharomyces cerevisiae growth.","authors":"Klára Papoušková, Marcos Gómez, Marie Kodedová, José Ramos, Olga Zimmermannová, Hana Sychrová","doi":"10.1002/yea.3834","DOIUrl":"https://doi.org/10.1002/yea.3834","url":null,"abstract":"In the model yeast Saccharomyces cerevisiae, Trk1 is the main K+ importer. It is involved in many important physiological processes, such as the maintenance of ion homeostasis, cell volume, intracellular pH, and plasma-membrane potential. The ScTrk1 protein can be of great interest to industry, as it was shown that changes in its activity influence ethanol production and tolerance in S. cerevisiae and also cell performance in the presence of organic acids or high ammonium under low K+ conditions. Nonconventional yeast species are attracting attention due to their unique properties and as a potential source of genes that encode proteins with unusual characteristics. In this work, we aimed to study and compare Trk proteins from Debaryomyces hansenii, Hortaea werneckii, Kluyveromyces marxianus, and Yarrowia lipolytica, four biotechnologically relevant yeasts that tolerate various extreme environments. Heterologous expression in S. cerevisiae cells lacking the endogenous Trk importers revealed differences in the studied Trk proteins' abilities to support the growth of cells under various cultivation conditions such as low K+ or the presence of toxic cations, to reduce plasma-membrane potential or to take up Rb+ . Examination of the potential of Trks to support the stress resistance of S. cerevisiae wild-type strains showed that Y. lipolytica Trk1 is a promising tool for improving cell tolerance to both low K+ and high salt and that the overproduction of S. cerevisiae's own Trk1 was the most efficient at improving the growth of cells in the presence of highly toxic Li+ ions.","PeriodicalId":23870,"journal":{"name":"Yeast","volume":"40 2","pages":"68-83"},"PeriodicalIF":2.6,"publicationDate":"2023-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9087968","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Brazilian Amazonian rainforest harbors a high diversity of yeasts associated with rotting wood, including many candidates for new yeast species. 巴西亚马逊雨林蕴藏着与腐烂木材相关的多种多样的酵母，包括许多新酵母物种的候选者。

IF 2.6 4区生物学

Yeast Pub Date : 2023-02-01 DOI: 10.1002/yea.3837

Katharina O Barros, Flávia B M Alvarenga, Giulia Magni, Gisele F L Souza, Maxwel A Abegg, Fernanda Palladino, Sílvio S da Silva, Rita C L B Rodrigues, Trey K Sato, Chris Todd Hittinger, Carlos A Rosa

{"title":"The Brazilian Amazonian rainforest harbors a high diversity of yeasts associated with rotting wood, including many candidates for new yeast species.","authors":"Katharina O Barros, Flávia B M Alvarenga, Giulia Magni, Gisele F L Souza, Maxwel A Abegg, Fernanda Palladino, Sílvio S da Silva, Rita C L B Rodrigues, Trey K Sato, Chris Todd Hittinger, Carlos A Rosa","doi":"10.1002/yea.3837","DOIUrl":"https://doi.org/10.1002/yea.3837","url":null,"abstract":"This study investigated the diversity of yeast species associated with rotting wood in Brazilian Amazonian rainforests. A total of 569 yeast strains were isolated from rotting wood samples collected in three Amazonian areas (Universidade Federal do Amazonas-Universidade Federal do Amazonas [UFAM], Piquiá, and Carú) in the municipality of Itacoatiara, Amazon state. The samples were cultured in yeast nitrogen base (YNB)-d-xylose, YNB-xylan, and sugarcane bagasse and corncob hemicellulosic hydrolysates (undiluted and diluted 1:2 and 1:5). Sugiyamaella was the most prevalent genus identified in this work, followed by Kazachstania. The most frequently isolated yeast species were Schwanniomyces polymorphus, Scheffersomyces amazonensis, and Wickerhamomyces sp., respectively. The alpha diversity analyses showed that the dryland forest of UFAM was the most diverse area, while the floodplain forest of Carú was the least. Additionally, the difference in diversity between UFAM and Carú was the highest among the comparisons. Thirty candidates for new yeast species were obtained, representing 36% of the species identified and totaling 101 isolates. Among them were species belonging to the clades Spathaspora, Scheffersomyces, and Sugiyamaella, which are recognized as genera with natural xylose-fermenting yeasts that are often studied for biotechnological and ecological purposes. The results of this work showed that rotting wood collected from the Amazonian rainforest is a tremendous source of diverse yeasts, including candidates for new species.","PeriodicalId":23870,"journal":{"name":"Yeast","volume":"40 2","pages":"84-101"},"PeriodicalIF":2.6,"publicationDate":"2023-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9072928","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 6

Issue Information 问题信息

IF 2.6 4区生物学

Yeast Pub Date : 2023-02-01 DOI: 10.1111/mila.12424

引用次数: 0

Linoleic acid functions as a quorum-sensing molecule in Monascus purpureus-Saccharomyces cerevisiae co-culture. 亚油酸作为群体感应分子在红曲霉与酿酒酵母共培养中的作用。

IF 2.6 4区生物学

Yeast Pub Date : 2023-01-01 DOI: 10.1002/yea.3831

Ruoyu Shi, Pengfei Gong, Yutong Liu, Qiaoqiao Luo, Wei Chen, Chengtao Wang

{"title":"Linoleic acid functions as a quorum-sensing molecule in Monascus purpureus-Saccharomyces cerevisiae co-culture.","authors":"Ruoyu Shi, Pengfei Gong, Yutong Liu, Qiaoqiao Luo, Wei Chen, Chengtao Wang","doi":"10.1002/yea.3831","DOIUrl":"https://doi.org/10.1002/yea.3831","url":null,"abstract":"When Monascus purpureus was co-cultured with Saccharomyces cerevisiae, we noted significant changes in the secondary metabolism and morphological development of Monascus. In yeast co-culture, although the pH was not different from that of a control, the Monascus mycelial biomass increased during fermentation, and the Monacolin K yield was significantly enhanced (up to 58.87% higher). However, pigment production did not increase. Co-culture with S. cerevisiae significantly increased the expression levels of genes related to Monacolin K production (mokA-mokI), especially mokE, mokF, and mokG. Linoleic acid, that has been implicated in playing a regulating role in the secondary metabolism and morphology of Monascus, was hypothesized to be the effector. Linoleic acid was detected in the co-culture, and its levels changed during fermentation. Addition of linoleic acid increased Monacolin K production and caused similar morphological changes in Monascus spores and mycelia. Exogenous linoleic acid also significantly upregulated the transcription levels of all nine genes involved in the biosynthesis of Monacolin K (up to 69.50% higher), consistent with the enhanced Monacolin K yield. Taken together, our results showed the effect of S. cerevisiae co-culture on M. purpureus and suggested linoleic acid as a specific quorum-sensing molecule in Saccharomyces-Monascus co-culture.","PeriodicalId":23870,"journal":{"name":"Yeast","volume":"40 1","pages":"42-52"},"PeriodicalIF":2.6,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9087956","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Da Vinci's yeast: Blastobotrys davincii f.a., sp. nov. 达氏酵母：达氏芽管酵母。

IF 2.6 4区生物学

Yeast Pub Date : 2023-01-01 DOI: 10.1002/yea.3816

Cobus M Visagie, Teun Boekhout, Bart Theelen, Jan Dijksterhuis, Neriman Yilmaz, Keith A Seifert

{"title":"Da Vinci's yeast: Blastobotrys davincii f.a., sp. nov.","authors":"Cobus M Visagie, Teun Boekhout, Bart Theelen, Jan Dijksterhuis, Neriman Yilmaz, Keith A Seifert","doi":"10.1002/yea.3816","DOIUrl":"https://doi.org/10.1002/yea.3816","url":null,"abstract":"A new species of the yeast genus Blastobotrys was discovered during a worldwide survey of culturable xerophilic fungi in house dust. Several culture-dependent and independent studies from around the world detected the same species from a wide range of substrates including indoor air, cave wall paintings, bats, mummies, and the iconic self-portrait of Leonardo da Vinci from ca 1512. However, none of these studies identified their strains, clones, or OTUs as Blastobotrys. We introduce the new species as Blastobotrys davincii f.a., sp. nov. (holotype CBS H-24879) and delineate it from other species using morphological, phylogenetic, and physiological characters. The new species of asexually (anamorphic) budding yeast is classified in Trichomonascaceae and forms a clade along with its associated sexual state genus Trichomonascus. Despite the decade-old requirement to use a single generic name for fungi, both names are still used. Selection of the preferred name awaits a formal nomenclatural proposal. We present arguments for adopting Blastobotrys over Trichomonascus and introduce four new combinations as Blastobotrys allociferrii (≡ Candida allociferrii), B. fungorum (≡ Sporothrix fungorum), B. mucifer (≡ Candida mucifera), and Blastobotrys vanleenenianus (≡ Trichomonascus vanleenenianus). We provide a nomenclatural review and an accepted species list for the 37 accepted species in the Blastobotrys/Trichomonascus clade. Finally, we discuss the identity of the DNA clones detected on the da Vinci portrait, and the importance of using appropriate media to isolate xerophilic or halophilic fungi.","PeriodicalId":23870,"journal":{"name":"Yeast","volume":"40 1","pages":"7-31"},"PeriodicalIF":2.6,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/63/e4/YEA-40-7.PMC10108157.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9318776","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

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