Yeast最新文献

{"title":"Editorial: On the Special Issue \"Yeast Science in Central and Eastern Europe\".","authors":"Andriy A Sibirny, Terrance G Cooper, Hiroshi Takagi","doi":"10.1002/yea.3883","DOIUrl":"https://doi.org/10.1002/yea.3883","url":null,"abstract":"The first Polish Yeast Conference was held at the University of Rzeszow in South‐Eastern Poland. Investigators from all the major Polish cities, Warsaw, Wroclaw, Poznan, Cracow, Gdansk, Lodz, Lublin, and Rzeszow, were joined by international colleagues from the United States, Japan, Sweden, Belgium, France, United Kingdom, Slovakia, and Ukraine in eight scientific sessions. The 114 attendees presented lectures and posters covering a wide variety of current topics leading to a very stimulating and successful conference. The conference demonstrated the presence and great potential for further development of yeast research in Poland. Dr. Gianni Liti, Editor‐in‐Chief of the Journal Yeast generously provided the conference's participants the opportunity of contributing peer‐reviewed articles to the Yeast Special Issue “Yeast Science in Central and Eastern Europe”. The 13 manuscripts, most from Poland, covered many of the topics presented during the conference. P. Fickers (Belgium) and his Chinese colleagues described improved production of the sweetener, erythritol, in Yarrowia lipolytica emanating from activation of the alternative glycerol catabolic, known as dihydroxyacetone, pathway. Overexpression of DAK2 coding for dihydroxyacetone kinase together with genes TKL1 and TAL1 coding for transketolase and transaldolase, respectively, led to a strain with increased productivity and yield of erythritol in glycerol medium. The paper of T. Cooper and co‐authors from the United States described the mechanisms through which nitrogen catabolite repression‐sensitive transcription activator Gln3 and TorC1 kinase regulate and integrate transcriptional control of the GABA (g‐aminobutyric acid) shunt and retrograde pathway genes. TorC1 regulates the last three shunt genes in a Gln3‐dependent manner, whereas it does so in a Gln3‐independent manner for the first shunt GABA decarboxylase gene. GABA shunt and retrograde pathway gene expression increase in the presence of nickel, likely to cope with increased ROS that nickel detoxification generates. H. Takagi and colleagues from Japan showed that arginine permease, Can1, beyond being an arginine transporter, also acts as an arginine transceptor that participates in the inhibition of proline utilization. They also reported that arginine activates protein kinase A signaling via the Can1 permease. The review of A. Tyczewska and K. Grzywacz from Poznan (Poland), assembled data showing that tRNA fragments are responsible for numerous regulatory functions in yeast. While the regulatory role of microRNAs and small interfering RNAs has been known for many years, recent studies demonstrated that specific tRNA fragments (tRFs) also affect gene expression in pathogenic and non‐pathogenic yeasts. D. Satala et al. from Cracow (Poland) described interactions between Candida parapsilosis adhesins, a family of the agglutinin‐like sequence protein, and human cells. These interactions were shown to be enhanced in the presence of fibr","PeriodicalId":23870,"journal":{"name":"Yeast","volume":"40 8","pages":"281-282"},"PeriodicalIF":2.6,"publicationDate":"2023-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10334351","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sodium and lithium exert differential effects on the central carbon metabolism of Debaryomyces hansenii through the glyoxylate shunt regulation.","authors":"Francisco S Ruiz-Pérez, Francisco J Ruiz-Castilla, Carlos Leal, José L Martínez, José Ramos","doi":"10.1002/yea.3856","DOIUrl":"10.1002/yea.3856","url":null,"abstract":"<p><p>Debaryomyces hansenii is a halotolerant/halophilic yeast usually found in salty environments. The yeast accumulated sodium at high concentrations, which improved growth in salty media. In contrast, lithium was toxic even at low concentrations and its presence prevented cell proliferation. To analyse the responses to both cations, metabolite levels, enzymatic activities and gene expression were determined, showing that NaCl and LiCl trigger different cellular responses. At high concentrations of NaCl (0.5 or 1.5 M) cells accumulated higher amounts of the intermediate metabolites glyoxylate and malate and, at the same time, the levels of intracellular oxoglutarate decreased. Additionally, 0.5 M NaCl increased the activity of the enzymes isocitrate lyase and malate synthase involved in the synthesis of glyoxylate and malate respectively and decreased the activity of isocitrate dehydrogenase. Moreover, transcription of the genes coding for isocitrate lyase and malate synthase was activated by NaCl. Also, cells accumulated phosphate upon NaCl exposure. None of these effects was provoked when LiCl (0.1 or 0.3 M) was used instead of NaCl. Lithium induced accumulation of higher amounts of oxoglutarate and decreased the concentrations of glyoxylate and malate to non-detectable levels. Cells incubated with lithium also showed higher activity of the isocitrate dehydrogenase and neither increased isocitrate lyase and malate synthase activities nor the transcription of the corresponding genes. In summary, we show that sodium, but not lithium, up regulates the shunt of the glyoxylic acid in D. hansenii and we propose that this is an important metabolic adaptation to thrive in salty environments.</p>","PeriodicalId":23870,"journal":{"name":"Yeast","volume":"40 7","pages":"265-275"},"PeriodicalIF":2.2,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10168826","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enrico Cabib (1925-2023).","authors":"Vladimír Farkaš,&nbsp;Javier Arroyo","doi":"10.1002/yea.3882","DOIUrl":"https://doi.org/10.1002/yea.3882","url":null,"abstract":"","PeriodicalId":23870,"journal":{"name":"Yeast","volume":"40 7","pages":"235-236"},"PeriodicalIF":2.6,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10153324","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Schizosaccharomyces lindneri sp. nov., a fission yeast occurring in honey.","authors":"Michael Brysch-Herzberg, Guo-Song Jia, Matthias Sipiczki, Martin Seidel, Wen Li, Imen Assali, Li-Lin Du","doi":"10.1002/yea.3857","DOIUrl":"10.1002/yea.3857","url":null,"abstract":"<p><p>Two strains of fission yeast were isolated from honey. They differ from the type strain of Schizosaccharomyces octosporus by three substitutions in the D1/D2 domain of the nuclear 26S large subunit ribosomal RNA (rRNA) gene sequence, resulting in a 99.5% identity. In the internal transcribed spacer (ITS) region (consisting of ITS1, 5.8S rDNA, and ITS2), the strains differ from S. octosporus by 16 gaps and 91 substitutions, which is equivalent to an identity of 88.1%. Genome sequencing on one of the new strains revealed that the average nucleotide identity (ANI) between its genome and the reference genome of S. octosporus is 90.43% and there exist major genome rearrangements between the two genomes. Mating analysis revealed that S. octosporus and one of the new strains are completely reproductively separated. A strong prezygotic barrier exists and the few mating products consist of diploid hybrids that do not form recombinant ascospores. In the new strains, asci are either zygotic, arising from conjugation, or they develop without conjugation from asexual cells (azygotic). Compared to the currently recognized Schizosaccharomyces species, the spectrum of nutrients that are assimilated by the new strains is restricted. Of the 43 carbohydrates that were included in the physiological standard tests, only 7 were assimilated. According to the results of the genome sequence analysis, the mating trials, and the phenotypic characterization, the new species Schizosaccharomyces lindneri is described to accommodate the two strains (holotype: CBS 18203^T  and ex-type: MUCL 58363; MycoBank no.: MB 847838).</p>","PeriodicalId":23870,"journal":{"name":"Yeast","volume":"40 7","pages":"237-253"},"PeriodicalIF":2.2,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9794308","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Metabolomics of astaxanthin biosynthesis and corresponding regulation strategies in Phaffia rhodozyma.","authors":"Haoyi Yang, Liang Yang, Xiping Du, Ning He, Zedong Jiang, Yanbing Zhu, Lijun Li, Hui Ni, Qingbiao Li, Zhipeng Li","doi":"10.1002/yea.3854","DOIUrl":"10.1002/yea.3854","url":null,"abstract":"<p><p>Astaxanthin is a valuable carotenoid and is used as antioxidant and health care. Phaffia rhodozyma is a potential strain for the biosynthesis of astaxanthin. The unclear metabolic characteristics of P. rhodozyma at different metabolic stages hinder astaxanthin's promotion. This study is conducted to investigate metabolite changes based on quadrupole time-of-flight mass spectrometry metabolomics method. The results showed that the downregulation of purine, pyrimidine, amino acid synthesis, and glycolytic pathways contributed to astaxanthin biosynthesis. Meanwhile, the upregulation of lipid metabolites contributed to astaxanthin accumulation. Therefore, the regulation strategies were proposed based on this. The addition of sodium orthovanadate inhibited the amino acid pathway to increase astaxanthin concentration by 19.2%. And the addition of melatonin promoted lipid metabolism to increase the astaxanthin concentration by 30.3%. It further confirmed that inhibition of amino acid metabolism and promotion of lipid metabolism were beneficial for astaxanthin biosynthesis of P. rhodozyma. It is helpful in understanding metabolic pathways affecting astaxanthin of P. rhodozyma and provides regulatory strategies for metabolism.</p>","PeriodicalId":23870,"journal":{"name":"Yeast","volume":"40 7","pages":"254-264"},"PeriodicalIF":2.2,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9792406","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Issue Information","authors":"","doi":"10.1002/yea.3757","DOIUrl":"https://doi.org/10.1002/yea.3757","url":null,"abstract":"No abstract is available for this article.","PeriodicalId":23870,"journal":{"name":"Yeast","volume":" ","pages":""},"PeriodicalIF":2.6,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48255644","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Yeast: A platform for the production of _L -tyrosine derivatives.","authors":"Xufan Guo, Xinxin Wu, He Ma, Huayi Liu, Yunzi Luo","doi":"10.1002/yea.3850","DOIUrl":"10.1002/yea.3850","url":null,"abstract":"<p><p>_L -Tyrosine derivatives are widely applied in the pharmaceutical, food, and chemical industries. Their production is mainly confined to chemical synthesis and plant extract. Microorganisms, as cell factories, exhibit promising advantages for valuable chemical production to fulfill the increase in the demand of global markets. Yeast has been used to produce natural products owing to its robustness and genetic maneuverability. Focusing on the progress of yeast cell factories for the production of _L -tyrosine derivatives, we summarized the emerging metabolic engineering approaches in building _L -tyrosoine-overproducing yeast and constructing cell factories of three typical chemicals and their derivatives: tyrosol, p-coumaric acid, and _L -DOPA. Finally, the challenges and opportunities of _L -tyrosine derivatives production in yeast cell factories were also discussed.</p>","PeriodicalId":23870,"journal":{"name":"Yeast","volume":"40 5-6","pages":"214-230"},"PeriodicalIF":2.2,"publicationDate":"2023-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9659575","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Diversity of dung beetle-associated yeasts from pristine environments of Botswana.","authors":"Anita E Nwaefuna, Teun Boekhout, Mar Garcia-Aloy, Urska Vrhovsek, Nerve Zhou","doi":"10.1002/yea.3852","DOIUrl":"10.1002/yea.3852","url":null,"abstract":"<p><p>Yeast-insect interactions are increasingly becoming an attractive source of discovery for previously unknown, unique, diverse, and industrially relevant yeast species. Despite a wealth of studies that have recently focused on yeasts in symbiotic association with Hymenopteran insects, yeasts associated with Coleopteran insects, such as lignocellulosic-rich dung-dependent beetles, remain poorly studied. Trends in yeast discovery suggest that species richness and diversity can be attributed to the ecological niche of the insect. Here, we considered the potential of dung beetles inhabiting the extreme environments of Botswana, characterized by desert-like conditions (semi-arid to arid and hot) as well as protected pristine environments, as possible attribute niches that can shape the extremophilic and diverse life history strategies of yeasts. We obtained a total of 97 phylogenetically diverse yeast isolates from six species of dung beetles from Botswana's unexplored environments, representing 19 species belonging to 11 genera. The findings suggest that the guts of dung beetles are a rich niche for non-Saccharomyces yeast species. Meyerozyma and Pichia were the most dominant genera associated with dung beetles, representing 55% (53 out of 97) of the yeast isolates in our study. Trichosporon and Cutaneotrichosporon genera represented 32% (31 out of 97) of the isolates. The remaining isolates belonged to Apiotrichum, Candida, Diutina, Naganishia, Rhodotorula, and Wickerhamiella genera (12 out of 97). We found out that about 62% (60 out of 97) of the isolates were potentially new species because of their low internal transcribed spacer (ITS) sequence similarity when compared to the most recent optimal species delineation threshold. A single isolate was unidentifiable using the ITS sequences. Using an in silico polymerase chain reaction-restriction fragment length polymorphism approach, we revealed that there was genetic diversity within isolates of the same species. Our results contribute to the knowledge and understanding of the diversity of dung beetle-associated yeasts.</p>","PeriodicalId":23870,"journal":{"name":"Yeast","volume":"40 5-6","pages":"182-196"},"PeriodicalIF":2.2,"publicationDate":"2023-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9978061","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Assessment of yeast physiology during industrial-scale brewing practices using the redox-sensitive dye resazurin.","authors":"Brooke A Dilmetz, Christopher T Desire, Leigh Donnellan, Jon Meneses, Manuela Klingler-Hoffmann, Clifford Young, Peter Hoffmann","doi":"10.1002/yea.3851","DOIUrl":"10.1002/yea.3851","url":null,"abstract":"<p><p>Beer refermentation in bottles is an industrial process utilized by breweries where yeast and fermentable extract are added to green beer. The beer is refermented for a minimum of 2 weeks before distribution, with the physiological state of the yeast a critical factor for successful refermentation. Ideally, fresh yeast that is propagated from a dedicated propagation plant should be used for refermentation in bottles. Here, we explored the applicability of the fluorescent and redox-sensitive dye, resazurin, to assess cellular metabolism in yeast and its ability to differentiate between growth stages. We applied this assay, with other markers of yeast physiology, to evaluate yeast quality during a full-scale industrial propagation. Resazurin allowed the discrimination between the different growth phases in yeast and afforded a more in-depth understanding of yeast metabolism during propagation. This assay can be used to optimize the yeast propagation process and cropping time to improve beer quality.</p>","PeriodicalId":23870,"journal":{"name":"Yeast","volume":"40 5-6","pages":"171-181"},"PeriodicalIF":2.2,"publicationDate":"2023-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9659125","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Assembly and comparative genome analysis of a Patagonian Aureobasidium pullulans isolate reveals unexpected intraspecific variation.","authors":"Micaela Parra, Diego Libkind, Chris Todd Hittinger, Lucía Álvarez, Nicolás Bellora","doi":"10.1002/yea.3853","DOIUrl":"10.1002/yea.3853","url":null,"abstract":"<p><p>Aureobasidium pullulans is a yeast-like fungus with remarkable phenotypic plasticity widely studied for its importance for the pharmaceutical and food industries. So far, genomic studies with strains from all over the world suggest they constitute a genetically unstructured population, with no association by habitat. However, the mechanisms by which this genome supports so many phenotypic permutations are still poorly understood. Recent works have shown the importance of sequencing yeast genomes from extreme environments to increase the repertoire of phenotypic diversity of unconventional yeasts. In this study, we present the genomic draft of A. pullulans strain from a Patagonian yeast diversity hotspot, re-evaluate its taxonomic classification based on taxogenomic approaches, and annotate its genome with high-depth transcriptomic data. Our analysis suggests this isolate could be considered a novel variant at an early stage of the speciation process. The discovery of divergent strains in a genomically homogeneous group, such as A. pullulans, can be valuable in understanding the evolution of the species. The identification and characterization of new variants will not only allow finding unique traits of biotechnological importance, but also optimize the choice of strains whose phenotypes will be characterized, providing new elements to explore questions about plasticity and adaptation.</p>","PeriodicalId":23870,"journal":{"name":"Yeast","volume":"40 5-6","pages":"197-213"},"PeriodicalIF":2.2,"publicationDate":"2023-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9659613","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}