Yeast最新文献

{"title":"The arginine transporter Can1 acts as a transceptor for regulation of proline utilization in the yeast Saccharomyces cerevisiae.","authors":"Ryoya Tanahashi,&nbsp;Akira Nishimura,&nbsp;Fumika Morita,&nbsp;Hayate Nakazawa,&nbsp;Atsuki Taniguchi,&nbsp;Kazuki Ichikawa,&nbsp;Kazuki Nakagami,&nbsp;Kyria Boundy-Mills,&nbsp;Hiroshi Takagi","doi":"10.1002/yea.3836","DOIUrl":"https://doi.org/10.1002/yea.3836","url":null,"abstract":"<p><p>Proline is the most abundant amino acid in wine and beer, because the yeast Saccharomyces cerevisiae hardly assimilates proline during fermentation processes. Our previous studies showed that arginine induces endocytosis of the proline transporter Put4, resulting in inhibition of proline utilization. We here report a possible role of arginine sensing in the inhibition of proline utilization. We first found that two basic amino acids, ornithine, and lysine, inhibit proline utilization by inducing Put4 endocytosis in a manner similar to arginine, but citrulline does not. Our genetic screening revealed that the arginine transporter Can1 is involved in the inhibition of proline utilization by arginine. Intriguingly, the arginine uptake activity of Can1 was not required for the arginine-dependent inhibition of proline utilization, suggesting that Can1 has a function beyond its commonly known function of transporting arginine. More importantly, our biochemical analyses revealed that Can1 activates signaling cascades of protein kinase A in response to extracellular arginine. Hence, we proposed that Can1 regulates proline utilization by functioning as a transceptor possessing the activity of both a transporter and receptor of arginine.</p>","PeriodicalId":23870,"journal":{"name":"Yeast","volume":"40 8","pages":"333-348"},"PeriodicalIF":2.6,"publicationDate":"2023-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9981153","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Oleaginous yeasts for biochemicals, biofuels and food from lignocellulose-hydrolysate and crude glycerol.","authors":"Volkmar Passoth,&nbsp;Jule Brandenburg,&nbsp;Mikołaj Chmielarz,&nbsp;Giselle Martín-Hernandez,&nbsp;Yashaswini Nagaraj,&nbsp;Bettina Müller,&nbsp;Johanna Blomqvist","doi":"10.1002/yea.3838","DOIUrl":"https://doi.org/10.1002/yea.3838","url":null,"abstract":"<p><p>Microbial lipids produced from lignocellulose and crude glycerol (CG) can serve as sustainable alternatives to vegetable oils, whose production is, in many cases, accompanied by monocultures, land use changes or rain forest clearings. Our projects aim to understand the physiology of microbial lipid production by oleaginous yeasts, optimise the production and establish novel applications of microbial lipid compounds. We have established methods for fermentation and intracellular lipid quantification. Following the kinetics of lipid accumulation in different strains, we found high variability in lipid formation even between very closely related oleaginous yeast strains on both, wheat straw hydrolysate and CG. For example, on complete wheat straw hydrolysate, we saw that one Rhodotorula glutinis strain, when starting assimilating D-xylosealso assimilated the accumulated lipids, while a Rhodotorula babjevae strain could accumulate lipids on D-xylose. Two strains (Rhodotorula toruloides CBS 14 and R. glutinis CBS 3044) were found to be the best out of 27 tested to accumulate lipids on CG. Interestingly, the presence of hemicellulose hydrolysate stimulated glycerol assimilation in both strains. Apart from microbial oil, R. toruloides also produces carotenoids. The first attempts of extraction using the classical acetone-based method showed that β-carotene is the major carotenoid. However, there are indications that there are also substantial amounts of torulene and torularhodin, which have a very high potential as antioxidants.</p>","PeriodicalId":23870,"journal":{"name":"Yeast","volume":"40 8","pages":"290-302"},"PeriodicalIF":2.6,"publicationDate":"2023-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9980834","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Release of neutrophil extracellular traps in response to Candida albicans yeast, as a secondary defense mechanism activated by phagocytosis.","authors":"Marcin Zawrotniak,&nbsp;Magdalena Juszczak,&nbsp;Maria Rapała-Kozik","doi":"10.1002/yea.3842","DOIUrl":"https://doi.org/10.1002/yea.3842","url":null,"abstract":"<p><p>Candida albicans is one of the main pathogens responsible for the development of difficult-to-fight fungal infections called candidiasis. Neutrophils are the major effector cells involved in the eradication of fungal pathogens. This group of immune cells uses several mechanisms that enable the rapid neutralization of pathogens. The most frequently identified mechanisms are phagocytosis and the release of neutrophil extracellular traps (NETs). The mechanism for selecting the type of neutrophil immune response is still unknown. In our study, we analyzed the relationship between the activation of phagocytosis and netosis. We detected the presence of two neutrophil populations characterized by different response patterns to contact with C. albicans blastospores. The first neutrophil population showed an increased ability to rapidly release NETs without prior internalization of the pathogen. In the second population, the netosis process was inherently associated with phagocytosis. Differences between populations also referred to the production of reactive oxygen species. Our results suggest that neutrophils use different strategies to fight C. albicans and, contrary to previous reports, these mechanisms are not mutually exclusive.</p>","PeriodicalId":23870,"journal":{"name":"Yeast","volume":"40 8","pages":"349-359"},"PeriodicalIF":2.6,"publicationDate":"2023-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10035240","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Construction of the advanced flavin mononucleotide producers in the flavinogenic yeast Candida famata.","authors":"Dariya V Fedorovych,&nbsp;Andriy O Tsyrulnyk,&nbsp;Justyna Ruchala,&nbsp;Svitlana M Sobchuk,&nbsp;Kostyantyn V Dmytruk,&nbsp;Lyubov R Fayura,&nbsp;Andriy A Sibirny","doi":"10.1002/yea.3843","DOIUrl":"https://doi.org/10.1002/yea.3843","url":null,"abstract":"<p><p>Flavin mononucleotide (FMN, riboflavin-5'-phosphate) is flavin coenzyme synthesized in all living organisms from riboflavin (vitamin B₂ ) after phosphorylation in the reaction catalyzed by riboflavin kinase. FMN has several applications mostly as yellow colorant in food industry due to 200 times better water solubility as compared to riboflavin. Currently, FMN is produced by chemical phosphorylation of riboflavin, however, final product contains up to 25% of flavin impurities. Microbial overproducers of FMN are known, however, they accumulate this coenzyme in glucose medium. Current work shows that the recombinant strains of the flavinogenic yeast Candida famata with overexpressed FMN1 gene coding for riboflavin kinase in the recently isolated by us advanced riboflavin producers due to overexpression of the structural and regulatory genes of riboflavin synthesis and of the putative exporter of riboflavin from the cell, synthesized elevated amounts of FMN in the media not only with glucose but also in lactose and cheese whey. Activation of FMN accumulation on lactose and cheese whey was especially strong in the strains which expressed the gene of transcription activator SEF1 under control of the lactose-induced LAC4 promoter. The accumulation of this coenzyme by the washed cells of the best recombinant strain achieved 540 mg/L in the cheese whey supplemented only with ammonium sulfate during 48 h in shake flask experiments.</p>","PeriodicalId":23870,"journal":{"name":"Yeast","volume":"40 8","pages":"360-366"},"PeriodicalIF":2.6,"publicationDate":"2023-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10035241","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"tRNA-derived fragments as new players in regulatory processes in yeast.","authors":"Agata Tyczewska,&nbsp;Kamilla Grzywacz","doi":"10.1002/yea.3829","DOIUrl":"https://doi.org/10.1002/yea.3829","url":null,"abstract":"<p><p>For a very long time, RNA molecules were treated as transistory molecules, by which the genetic information flows from DNA to proteins; the model proposed in the 1960s accepted that proteins are both the products and the regulators of gene expression. Since then, thousands of reports proved that RNAs should be thought about as the factors that do control gene expression. The pervasive transcription has been reported in many eukaryotic organisms, illustrating a highly interwoven transcriptome organization that includes hundreds of previously unknown noncoding RNAs. The key roles of noncoding RNAs (microRNAs and small interfering RNAs) in gene expression regulation are no longer surprising, as are new classes of noncoding RNAs constantly being discovered. Transfer RNAs (tRNAs) are the second most abundant type of RNAs in the cell. Advances in high-throughput sequencing technologies exposed the existence of functional, regulatory tRNA-derived RNA fragments (tRFs), generated from precursor and mature tRNAs. These tRF molecules have been found to play central roles during stress and different pathological conditions. Herein, we present the critical assessment of the discoveries made in the field of tRNA-derived fragments in the past 15 years in various pathogenic and nonpathogenic yeast species.</p>","PeriodicalId":23870,"journal":{"name":"Yeast","volume":"40 8","pages":"283-289"},"PeriodicalIF":2.6,"publicationDate":"2023-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10333853","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Editorial: On the Special Issue \"Yeast Science in Central and Eastern Europe\".","authors":"Andriy A Sibirny,&nbsp;Terrance G Cooper,&nbsp;Hiroshi Takagi","doi":"10.1002/yea.3883","DOIUrl":"https://doi.org/10.1002/yea.3883","url":null,"abstract":"The first Polish Yeast Conference was held at the University of Rzeszow in South‐Eastern Poland. Investigators from all the major Polish cities, Warsaw, Wroclaw, Poznan, Cracow, Gdansk, Lodz, Lublin, and Rzeszow, were joined by international colleagues from the United States, Japan, Sweden, Belgium, France, United Kingdom, Slovakia, and Ukraine in eight scientific sessions. The 114 attendees presented lectures and posters covering a wide variety of current topics leading to a very stimulating and successful conference. The conference demonstrated the presence and great potential for further development of yeast research in Poland. Dr. Gianni Liti, Editor‐in‐Chief of the Journal Yeast generously provided the conference's participants the opportunity of contributing peer‐reviewed articles to the Yeast Special Issue “Yeast Science in Central and Eastern Europe”. The 13 manuscripts, most from Poland, covered many of the topics presented during the conference. P. Fickers (Belgium) and his Chinese colleagues described improved production of the sweetener, erythritol, in Yarrowia lipolytica emanating from activation of the alternative glycerol catabolic, known as dihydroxyacetone, pathway. Overexpression of DAK2 coding for dihydroxyacetone kinase together with genes TKL1 and TAL1 coding for transketolase and transaldolase, respectively, led to a strain with increased productivity and yield of erythritol in glycerol medium. The paper of T. Cooper and co‐authors from the United States described the mechanisms through which nitrogen catabolite repression‐sensitive transcription activator Gln3 and TorC1 kinase regulate and integrate transcriptional control of the GABA (g‐aminobutyric acid) shunt and retrograde pathway genes. TorC1 regulates the last three shunt genes in a Gln3‐dependent manner, whereas it does so in a Gln3‐independent manner for the first shunt GABA decarboxylase gene. GABA shunt and retrograde pathway gene expression increase in the presence of nickel, likely to cope with increased ROS that nickel detoxification generates. H. Takagi and colleagues from Japan showed that arginine permease, Can1, beyond being an arginine transporter, also acts as an arginine transceptor that participates in the inhibition of proline utilization. They also reported that arginine activates protein kinase A signaling via the Can1 permease. The review of A. Tyczewska and K. Grzywacz from Poznan (Poland), assembled data showing that tRNA fragments are responsible for numerous regulatory functions in yeast. While the regulatory role of microRNAs and small interfering RNAs has been known for many years, recent studies demonstrated that specific tRNA fragments (tRFs) also affect gene expression in pathogenic and non‐pathogenic yeasts. D. Satala et al. from Cracow (Poland) described interactions between Candida parapsilosis adhesins, a family of the agglutinin‐like sequence protein, and human cells. These interactions were shown to be enhanced in the presence of fibr","PeriodicalId":23870,"journal":{"name":"Yeast","volume":"40 8","pages":"281-282"},"PeriodicalIF":2.6,"publicationDate":"2023-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10334351","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sodium and lithium exert differential effects on the central carbon metabolism of Debaryomyces hansenii through the glyoxylate shunt regulation.","authors":"Francisco S Ruiz-Pérez, Francisco J Ruiz-Castilla, Carlos Leal, José L Martínez, José Ramos","doi":"10.1002/yea.3856","DOIUrl":"10.1002/yea.3856","url":null,"abstract":"<p><p>Debaryomyces hansenii is a halotolerant/halophilic yeast usually found in salty environments. The yeast accumulated sodium at high concentrations, which improved growth in salty media. In contrast, lithium was toxic even at low concentrations and its presence prevented cell proliferation. To analyse the responses to both cations, metabolite levels, enzymatic activities and gene expression were determined, showing that NaCl and LiCl trigger different cellular responses. At high concentrations of NaCl (0.5 or 1.5 M) cells accumulated higher amounts of the intermediate metabolites glyoxylate and malate and, at the same time, the levels of intracellular oxoglutarate decreased. Additionally, 0.5 M NaCl increased the activity of the enzymes isocitrate lyase and malate synthase involved in the synthesis of glyoxylate and malate respectively and decreased the activity of isocitrate dehydrogenase. Moreover, transcription of the genes coding for isocitrate lyase and malate synthase was activated by NaCl. Also, cells accumulated phosphate upon NaCl exposure. None of these effects was provoked when LiCl (0.1 or 0.3 M) was used instead of NaCl. Lithium induced accumulation of higher amounts of oxoglutarate and decreased the concentrations of glyoxylate and malate to non-detectable levels. Cells incubated with lithium also showed higher activity of the isocitrate dehydrogenase and neither increased isocitrate lyase and malate synthase activities nor the transcription of the corresponding genes. In summary, we show that sodium, but not lithium, up regulates the shunt of the glyoxylic acid in D. hansenii and we propose that this is an important metabolic adaptation to thrive in salty environments.</p>","PeriodicalId":23870,"journal":{"name":"Yeast","volume":"40 7","pages":"265-275"},"PeriodicalIF":2.2,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10168826","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enrico Cabib (1925-2023).","authors":"Vladimír Farkaš,&nbsp;Javier Arroyo","doi":"10.1002/yea.3882","DOIUrl":"https://doi.org/10.1002/yea.3882","url":null,"abstract":"","PeriodicalId":23870,"journal":{"name":"Yeast","volume":"40 7","pages":"235-236"},"PeriodicalIF":2.6,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10153324","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Schizosaccharomyces lindneri sp. nov., a fission yeast occurring in honey.","authors":"Michael Brysch-Herzberg, Guo-Song Jia, Matthias Sipiczki, Martin Seidel, Wen Li, Imen Assali, Li-Lin Du","doi":"10.1002/yea.3857","DOIUrl":"10.1002/yea.3857","url":null,"abstract":"<p><p>Two strains of fission yeast were isolated from honey. They differ from the type strain of Schizosaccharomyces octosporus by three substitutions in the D1/D2 domain of the nuclear 26S large subunit ribosomal RNA (rRNA) gene sequence, resulting in a 99.5% identity. In the internal transcribed spacer (ITS) region (consisting of ITS1, 5.8S rDNA, and ITS2), the strains differ from S. octosporus by 16 gaps and 91 substitutions, which is equivalent to an identity of 88.1%. Genome sequencing on one of the new strains revealed that the average nucleotide identity (ANI) between its genome and the reference genome of S. octosporus is 90.43% and there exist major genome rearrangements between the two genomes. Mating analysis revealed that S. octosporus and one of the new strains are completely reproductively separated. A strong prezygotic barrier exists and the few mating products consist of diploid hybrids that do not form recombinant ascospores. In the new strains, asci are either zygotic, arising from conjugation, or they develop without conjugation from asexual cells (azygotic). Compared to the currently recognized Schizosaccharomyces species, the spectrum of nutrients that are assimilated by the new strains is restricted. Of the 43 carbohydrates that were included in the physiological standard tests, only 7 were assimilated. According to the results of the genome sequence analysis, the mating trials, and the phenotypic characterization, the new species Schizosaccharomyces lindneri is described to accommodate the two strains (holotype: CBS 18203^T  and ex-type: MUCL 58363; MycoBank no.: MB 847838).</p>","PeriodicalId":23870,"journal":{"name":"Yeast","volume":"40 7","pages":"237-253"},"PeriodicalIF":2.2,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9794308","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Metabolomics of astaxanthin biosynthesis and corresponding regulation strategies in Phaffia rhodozyma.","authors":"Haoyi Yang, Liang Yang, Xiping Du, Ning He, Zedong Jiang, Yanbing Zhu, Lijun Li, Hui Ni, Qingbiao Li, Zhipeng Li","doi":"10.1002/yea.3854","DOIUrl":"10.1002/yea.3854","url":null,"abstract":"<p><p>Astaxanthin is a valuable carotenoid and is used as antioxidant and health care. Phaffia rhodozyma is a potential strain for the biosynthesis of astaxanthin. The unclear metabolic characteristics of P. rhodozyma at different metabolic stages hinder astaxanthin's promotion. This study is conducted to investigate metabolite changes based on quadrupole time-of-flight mass spectrometry metabolomics method. The results showed that the downregulation of purine, pyrimidine, amino acid synthesis, and glycolytic pathways contributed to astaxanthin biosynthesis. Meanwhile, the upregulation of lipid metabolites contributed to astaxanthin accumulation. Therefore, the regulation strategies were proposed based on this. The addition of sodium orthovanadate inhibited the amino acid pathway to increase astaxanthin concentration by 19.2%. And the addition of melatonin promoted lipid metabolism to increase the astaxanthin concentration by 30.3%. It further confirmed that inhibition of amino acid metabolism and promotion of lipid metabolism were beneficial for astaxanthin biosynthesis of P. rhodozyma. It is helpful in understanding metabolic pathways affecting astaxanthin of P. rhodozyma and provides regulatory strategies for metabolism.</p>","PeriodicalId":23870,"journal":{"name":"Yeast","volume":"40 7","pages":"254-264"},"PeriodicalIF":2.2,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9792406","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}