IF 2.6 4区生物学

Yeast Pub Date : 2023-09-01 Epub Date: 2023-08-10 DOI: 10.1002/yea.3891

Tingxiu Yang, Yuanyuan Zhang, Hua Zhang, Xiaojuan Wu, Jianchao Sun, Dengxiong Hua, Ke Pan, Qi Liu, Guzhen Cui, Zhenghong Chen

{"title":"Intracellular presence and genetic relationship of Helicobacter pylori within neonates' fecal yeasts and their mothers' vaginal yeasts.","authors":"Tingxiu Yang, Yuanyuan Zhang, Hua Zhang, Xiaojuan Wu, Jianchao Sun, Dengxiong Hua, Ke Pan, Qi Liu, Guzhen Cui, Zhenghong Chen","doi":"10.1002/yea.3891","DOIUrl":"10.1002/yea.3891","url":null,"abstract":"Helicobacter pylori are transmissible from person to person and among family members. Mother-to-child transmission is the main intrafamilial route of H. pylori transmission. However, how it transmits from mother to child is still being determined. Vaginal yeast often transmits to neonates during delivery. Therefore, H. pylori hosted in yeast might follow the same transmission route. This study aimed to detect intracellular H. pylori in vaginal and fecal yeasts isolates and explore the role of yeast in H. pylori transmission. Yeast was isolated from the mothers' vaginal discharge and neonates' feces and identified by internal transcribed spacer (ITS) sequencing. H. pylori 16S rRNA and antigen were detected in yeast isolates by polymerase chain reaction and direct immunofluorescence assay. Genetic relationships of Candida strains isolated from seven mothers and their corresponding neonates were determined by random amplified polymorphic DNA (RAPD) fingerprinting and ITS alignment. The Candida isolates from four mother-neonate pairs had identical RAPD patterns and highly homologous ITS sequences. The current study showed H. pylori could be sheltered within yeast colonizing the vagina, and fecal yeast from neonates is genetically related to the vaginal yeast from their mothers. Thus, vaginal yeast presents a potential reservoir of H. pylori and plays a vital role in the transmission from mother to neonate.","PeriodicalId":23870,"journal":{"name":"Yeast","volume":"40 9","pages":"401-413"},"PeriodicalIF":2.6,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10250580","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Komagatella phaffii ACG1 gene, encoding β-1,6-N-acetylglucosaminyltransferase, is involved in the autophagy of cytosolic and peroxisomal proteins. 法菲Komagatella phaffii ACG1基因编码β-1,6- n -乙酰氨基葡萄糖转移酶，参与胞浆蛋白和过氧化物酶体蛋白的自噬。

IF 2.6 4区生物学

Yeast Pub Date : 2023-08-01 DOI: 10.1002/yea.3846

Anastasiya Z Zazulya, Marta V Semkiv, Maxim Stec, Zuzanna Cyske, Lidia Gaffke, Karolina Pierzynowska, Grzegorz Węgrzyn, Andriy A Sibirny

{"title":"The Komagatella phaffii ACG1 gene, encoding β-1,6-N-acetylglucosaminyltransferase, is involved in the autophagy of cytosolic and peroxisomal proteins.","authors":"Anastasiya Z Zazulya, Marta V Semkiv, Maxim Stec, Zuzanna Cyske, Lidia Gaffke, Karolina Pierzynowska, Grzegorz Węgrzyn, Andriy A Sibirny","doi":"10.1002/yea.3846","DOIUrl":"https://doi.org/10.1002/yea.3846","url":null,"abstract":"The methylotrophic yeast Komagataella phaffii is considered one of the most effective producers of recombinant proteins of industrial importance. Effective producers should be characterized by the maximal reduction of degradation of the cytosolic recombinant proteins. The mechanisms of degradation of cytosolic proteins in K. phaffii have not been elucidated; however, data suggest that they are partially degraded in the autophagic pathway. To identify factors that influence this process, a developed system for the selection of recombinant strains of K. phaffii with impaired autophagic degradation of the heterologous model cytosolic protein (yeast β-galactosidase) was used for insertional tagging of the genes involved in cytosolic proteins degradation. In one of the obtained strains, the insertion cassette disrupted the open reading frame of the gene encoding β-1,6-N-acetylglucosaminyltransferase. A recombinant strain with deletion of this gene was also obtained. The rate of degradation of the β-galactosidase enzyme was two times slower in the insertion mutant and 1.5 times slower in the deletion strain as compared to the parental strain with native β-1,6-N-acetylglucosaminyltransferase. The rate of degradation of native K. phaffii cytosolic and peroxisomal enzymes, formaldehyde dehydrogenase, formate dehydrogenase, and alcohol oxidase, respectively, showed similar trends to that of β-galactosidase-slower degradation in the deletion and insertional mutants as compared to the wild-type strain, but faster protein degradation relative to the strain completely defective in autophagy. We conclude that K. phaffii gene designated ACG1, encoding β-1,6-N-acetylglucosaminyltransferase, is involved in autophagy of the cytosolic and peroxisomal proteins.","PeriodicalId":23870,"journal":{"name":"Yeast","volume":"40 8","pages":"367-376"},"PeriodicalIF":2.6,"publicationDate":"2023-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9980846","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

TorC1 and nitrogen catabolite repression control of integrated GABA shunt and retrograde pathway gene expression. TorC1和氮分解代谢产物对GABA旁路和逆行通路基因表达的抑制控制。

IF 2.2 4区生物学

Yeast Pub Date : 2023-08-01 Epub Date: 2023-04-10 DOI: 10.1002/yea.3849

Jennifer J Tate, Rajendra Rai, Terrance G Cooper

引用次数: 0

Living together: The role of Candida albicans in the formation of polymicrobial biofilms in the oral cavity. 共同生活:白色念珠菌在口腔内形成多微生物生物膜中的作用。

IF 2.6 4区生物学

Yeast Pub Date : 2023-08-01 DOI: 10.1002/yea.3855

Maria Rapala-Kozik, Magdalena Surowiec, Magdalena Juszczak, Ewelina Wronowska, Kamila Kulig, Aneta Bednarek, Miriam Gonzalez-Gonzalez, Justyna Karkowska-Kuleta, Marcin Zawrotniak, Dorota Satała, Andrzej Kozik

{"title":"Living together: The role of Candida albicans in the formation of polymicrobial biofilms in the oral cavity.","authors":"Maria Rapala-Kozik, Magdalena Surowiec, Magdalena Juszczak, Ewelina Wronowska, Kamila Kulig, Aneta Bednarek, Miriam Gonzalez-Gonzalez, Justyna Karkowska-Kuleta, Marcin Zawrotniak, Dorota Satała, Andrzej Kozik","doi":"10.1002/yea.3855","DOIUrl":"https://doi.org/10.1002/yea.3855","url":null,"abstract":"The oral cavity of humans is colonized by diversity of microbial community, although dominated by bacteria, it is also constituted by a low number of fungi, often represented by Candida albicans. Although in the vast minority, this usually commensal fungus under certain conditions of the host (e.g., immunosuppression or antibiotic therapy), can transform into an invasive pathogen that adheres to mucous membranes and also to medical or dental devices, causing mucosal infections. This transformation is correlated with changes in cell morphology from yeast-like cells to hyphae and is supported by numerous virulence factors exposed by C. albicans cells at the site of infection, such as multifunctional adhesins, degradative enzymes, or toxin. All of them affect the surrounding host cells or proteins, leading to their destruction. However, at the site of infection, C. albicans can interact with different bacterial species and in its filamentous form may produce biofilms-the elaborated consortia of microorganisms, that present increased ability to host colonization and resistance to antimicrobial agents. In this review, we highlight the modification of the infectious potential of C. albicans in contact with different bacterial species, and also consider the mutual bacterial-fungal relationships, involving cooperation, competition, or antagonism, that lead to an increase in the propagation of oral infection. The mycofilm of C. albicans is an excellent hiding place for bacteria, especially those that prefer low oxygen availability, where microbial cells during mutual co-existence can avoid host recognition or elimination by antimicrobial action. However, these microbial relationships, identified mainly in in vitro studies, are modified depending on the complexity of host conditions and microbial dominance in vivo.","PeriodicalId":23870,"journal":{"name":"Yeast","volume":"40 8","pages":"303-317"},"PeriodicalIF":2.6,"publicationDate":"2023-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10334344","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Candida parapsilosis cell wall proteins-CPAR2_404800 and CPAR2_404780-Are adhesins that bind to human epithelial and endothelial cells and extracellular matrix proteins. 假丝酵母旁裂症细胞壁蛋白cpar2_404800和cpar2_404780是结合人上皮细胞和内皮细胞及细胞外基质蛋白的粘附素。

IF 2.6 4区生物学

Yeast Pub Date : 2023-08-01 DOI: 10.1002/yea.3847

Dorota Satala, Justyna Karkowska-Kuleta, Grazyna Bras, Maria Rapala-Kozik, Andrzej Kozik

{"title":"Candida parapsilosis cell wall proteins-CPAR2_404800 and CPAR2_404780-Are adhesins that bind to human epithelial and endothelial cells and extracellular matrix proteins.","authors":"Dorota Satala, Justyna Karkowska-Kuleta, Grazyna Bras, Maria Rapala-Kozik, Andrzej Kozik","doi":"10.1002/yea.3847","DOIUrl":"https://doi.org/10.1002/yea.3847","url":null,"abstract":"One of the initial steps necessary for the development of Candida infections is the adherence to the host tissues and cells. Recent transcriptomic studies suggest that, in Candida parapsilosis—a fungal infectious agent that causes systemic candidiasis in immunosuppressed individuals—the adhesion is mediated by pathogen cell‐exposed proteins belonging to the agglutinin‐like sequence (Als) family. However, to date, the actual interactions of individual members of this family with human cells and extracellular matrix (ECM) have not been characterized in detail. In the current study, we focused attention on two of these C. parapsilosis Als proteins—CPAR2_404800 and CPAR2_404780—that were proteomically identified in the fungal cell wall of yeasts grown in the media suitable for culturing human epithelial and endothelial cells. Both proteins were extracted from the cell wall and purified, and using a microplate binding assay and a fluorescence microscopic analysis were shown to adhere to human cells of A431 (epithelial) and HMEC‐1 (endothelial) lines. The human extracellular matrix components that are also plasma proteins—fibronectin and vitronectin—enhanced these interactions, and also could directly bind to CPAR2_404800 and CPAR2_404780 proteins, with a high affinity (KD in a range of 10−7 to 10−8 M) as determined by surface plasmon resonance measurements. Our findings highlight the role of proteins CPAR2_404800 and CPAR2_404780 in adhesion to host cells and proteins, contributing to the knowledge of the mechanisms of host‐pathogen interactions during C. parapsilosis‐caused infections.","PeriodicalId":23870,"journal":{"name":"Yeast","volume":"40 8","pages":"377-389"},"PeriodicalIF":2.6,"publicationDate":"2023-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10333879","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The arginine transporter Can1 acts as a transceptor for regulation of proline utilization in the yeast Saccharomyces cerevisiae. 精氨酸转运体Can1在酿酒酵母中作为调节脯氨酸利用的受体。

IF 2.6 4区生物学

Yeast Pub Date : 2023-08-01 DOI: 10.1002/yea.3836

Ryoya Tanahashi, Akira Nishimura, Fumika Morita, Hayate Nakazawa, Atsuki Taniguchi, Kazuki Ichikawa, Kazuki Nakagami, Kyria Boundy-Mills, Hiroshi Takagi

{"title":"The arginine transporter Can1 acts as a transceptor for regulation of proline utilization in the yeast Saccharomyces cerevisiae.","authors":"Ryoya Tanahashi, Akira Nishimura, Fumika Morita, Hayate Nakazawa, Atsuki Taniguchi, Kazuki Ichikawa, Kazuki Nakagami, Kyria Boundy-Mills, Hiroshi Takagi","doi":"10.1002/yea.3836","DOIUrl":"https://doi.org/10.1002/yea.3836","url":null,"abstract":"Proline is the most abundant amino acid in wine and beer, because the yeast Saccharomyces cerevisiae hardly assimilates proline during fermentation processes. Our previous studies showed that arginine induces endocytosis of the proline transporter Put4, resulting in inhibition of proline utilization. We here report a possible role of arginine sensing in the inhibition of proline utilization. We first found that two basic amino acids, ornithine, and lysine, inhibit proline utilization by inducing Put4 endocytosis in a manner similar to arginine, but citrulline does not. Our genetic screening revealed that the arginine transporter Can1 is involved in the inhibition of proline utilization by arginine. Intriguingly, the arginine uptake activity of Can1 was not required for the arginine-dependent inhibition of proline utilization, suggesting that Can1 has a function beyond its commonly known function of transporting arginine. More importantly, our biochemical analyses revealed that Can1 activates signaling cascades of protein kinase A in response to extracellular arginine. Hence, we proposed that Can1 regulates proline utilization by functioning as a transceptor possessing the activity of both a transporter and receptor of arginine.","PeriodicalId":23870,"journal":{"name":"Yeast","volume":"40 8","pages":"333-348"},"PeriodicalIF":2.6,"publicationDate":"2023-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9981153","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

Oleaginous yeasts for biochemicals, biofuels and food from lignocellulose-hydrolysate and crude glycerol. 从木质纤维素水解物和粗甘油中提取生物化学、生物燃料和食品的产油酵母。

IF 2.6 4区生物学

Yeast Pub Date : 2023-08-01 DOI: 10.1002/yea.3838

Volkmar Passoth, Jule Brandenburg, Mikołaj Chmielarz, Giselle Martín-Hernandez, Yashaswini Nagaraj, Bettina Müller, Johanna Blomqvist

{"title":"Oleaginous yeasts for biochemicals, biofuels and food from lignocellulose-hydrolysate and crude glycerol.","authors":"Volkmar Passoth, Jule Brandenburg, Mikołaj Chmielarz, Giselle Martín-Hernandez, Yashaswini Nagaraj, Bettina Müller, Johanna Blomqvist","doi":"10.1002/yea.3838","DOIUrl":"https://doi.org/10.1002/yea.3838","url":null,"abstract":"Microbial lipids produced from lignocellulose and crude glycerol (CG) can serve as sustainable alternatives to vegetable oils, whose production is, in many cases, accompanied by monocultures, land use changes or rain forest clearings. Our projects aim to understand the physiology of microbial lipid production by oleaginous yeasts, optimise the production and establish novel applications of microbial lipid compounds. We have established methods for fermentation and intracellular lipid quantification. Following the kinetics of lipid accumulation in different strains, we found high variability in lipid formation even between very closely related oleaginous yeast strains on both, wheat straw hydrolysate and CG. For example, on complete wheat straw hydrolysate, we saw that one Rhodotorula glutinis strain, when starting assimilating D-xylosealso assimilated the accumulated lipids, while a Rhodotorula babjevae strain could accumulate lipids on D-xylose. Two strains (Rhodotorula toruloides CBS 14 and R. glutinis CBS 3044) were found to be the best out of 27 tested to accumulate lipids on CG. Interestingly, the presence of hemicellulose hydrolysate stimulated glycerol assimilation in both strains. Apart from microbial oil, R. toruloides also produces carotenoids. The first attempts of extraction using the classical acetone-based method showed that β-carotene is the major carotenoid. However, there are indications that there are also substantial amounts of torulene and torularhodin, which have a very high potential as antioxidants.","PeriodicalId":23870,"journal":{"name":"Yeast","volume":"40 8","pages":"290-302"},"PeriodicalIF":2.6,"publicationDate":"2023-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9980834","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

Release of neutrophil extracellular traps in response to Candida albicans yeast, as a secondary defense mechanism activated by phagocytosis. 释放中性粒细胞胞外陷阱响应白色念珠菌酵母菌，作为一个次级防御机制激活的吞噬。

IF 2.6 4区生物学

Yeast Pub Date : 2023-08-01 DOI: 10.1002/yea.3842

Marcin Zawrotniak, Magdalena Juszczak, Maria Rapała-Kozik

{"title":"Release of neutrophil extracellular traps in response to Candida albicans yeast, as a secondary defense mechanism activated by phagocytosis.","authors":"Marcin Zawrotniak, Magdalena Juszczak, Maria Rapała-Kozik","doi":"10.1002/yea.3842","DOIUrl":"https://doi.org/10.1002/yea.3842","url":null,"abstract":"Candida albicans is one of the main pathogens responsible for the development of difficult-to-fight fungal infections called candidiasis. Neutrophils are the major effector cells involved in the eradication of fungal pathogens. This group of immune cells uses several mechanisms that enable the rapid neutralization of pathogens. The most frequently identified mechanisms are phagocytosis and the release of neutrophil extracellular traps (NETs). The mechanism for selecting the type of neutrophil immune response is still unknown. In our study, we analyzed the relationship between the activation of phagocytosis and netosis. We detected the presence of two neutrophil populations characterized by different response patterns to contact with C. albicans blastospores. The first neutrophil population showed an increased ability to rapidly release NETs without prior internalization of the pathogen. In the second population, the netosis process was inherently associated with phagocytosis. Differences between populations also referred to the production of reactive oxygen species. Our results suggest that neutrophils use different strategies to fight C. albicans and, contrary to previous reports, these mechanisms are not mutually exclusive.","PeriodicalId":23870,"journal":{"name":"Yeast","volume":"40 8","pages":"349-359"},"PeriodicalIF":2.6,"publicationDate":"2023-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10035240","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Construction of the advanced flavin mononucleotide producers in the flavinogenic yeast Candida famata. 黄素产酵母菌中黄素单核苷酸先进产体的构建。

IF 2.6 4区生物学

Yeast Pub Date : 2023-08-01 DOI: 10.1002/yea.3843

Dariya V Fedorovych, Andriy O Tsyrulnyk, Justyna Ruchala, Svitlana M Sobchuk, Kostyantyn V Dmytruk, Lyubov R Fayura, Andriy A Sibirny

{"title":"Construction of the advanced flavin mononucleotide producers in the flavinogenic yeast Candida famata.","authors":"Dariya V Fedorovych, Andriy O Tsyrulnyk, Justyna Ruchala, Svitlana M Sobchuk, Kostyantyn V Dmytruk, Lyubov R Fayura, Andriy A Sibirny","doi":"10.1002/yea.3843","DOIUrl":"https://doi.org/10.1002/yea.3843","url":null,"abstract":"Flavin mononucleotide (FMN, riboflavin-5'-phosphate) is flavin coenzyme synthesized in all living organisms from riboflavin (vitamin B2 ) after phosphorylation in the reaction catalyzed by riboflavin kinase. FMN has several applications mostly as yellow colorant in food industry due to 200 times better water solubility as compared to riboflavin. Currently, FMN is produced by chemical phosphorylation of riboflavin, however, final product contains up to 25% of flavin impurities. Microbial overproducers of FMN are known, however, they accumulate this coenzyme in glucose medium. Current work shows that the recombinant strains of the flavinogenic yeast Candida famata with overexpressed FMN1 gene coding for riboflavin kinase in the recently isolated by us advanced riboflavin producers due to overexpression of the structural and regulatory genes of riboflavin synthesis and of the putative exporter of riboflavin from the cell, synthesized elevated amounts of FMN in the media not only with glucose but also in lactose and cheese whey. Activation of FMN accumulation on lactose and cheese whey was especially strong in the strains which expressed the gene of transcription activator SEF1 under control of the lactose-induced LAC4 promoter. The accumulation of this coenzyme by the washed cells of the best recombinant strain achieved 540 mg/L in the cheese whey supplemented only with ammonium sulfate during 48 h in shake flask experiments.","PeriodicalId":23870,"journal":{"name":"Yeast","volume":"40 8","pages":"360-366"},"PeriodicalIF":2.6,"publicationDate":"2023-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10035241","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

tRNA-derived fragments as new players in regulatory processes in yeast. trna衍生片段在酵母调控过程中的新作用。

IF 2.6 4区生物学

Yeast Pub Date : 2023-08-01 DOI: 10.1002/yea.3829

Agata Tyczewska, Kamilla Grzywacz

{"title":"tRNA-derived fragments as new players in regulatory processes in yeast.","authors":"Agata Tyczewska, Kamilla Grzywacz","doi":"10.1002/yea.3829","DOIUrl":"https://doi.org/10.1002/yea.3829","url":null,"abstract":"For a very long time, RNA molecules were treated as transistory molecules, by which the genetic information flows from DNA to proteins; the model proposed in the 1960s accepted that proteins are both the products and the regulators of gene expression. Since then, thousands of reports proved that RNAs should be thought about as the factors that do control gene expression. The pervasive transcription has been reported in many eukaryotic organisms, illustrating a highly interwoven transcriptome organization that includes hundreds of previously unknown noncoding RNAs. The key roles of noncoding RNAs (microRNAs and small interfering RNAs) in gene expression regulation are no longer surprising, as are new classes of noncoding RNAs constantly being discovered. Transfer RNAs (tRNAs) are the second most abundant type of RNAs in the cell. Advances in high-throughput sequencing technologies exposed the existence of functional, regulatory tRNA-derived RNA fragments (tRFs), generated from precursor and mature tRNAs. These tRF molecules have been found to play central roles during stress and different pathological conditions. Herein, we present the critical assessment of the discoveries made in the field of tRNA-derived fragments in the past 15 years in various pathogenic and nonpathogenic yeast species.","PeriodicalId":23870,"journal":{"name":"Yeast","volume":"40 8","pages":"283-289"},"PeriodicalIF":2.6,"publicationDate":"2023-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10333853","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

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