Yeast最新文献

{"title":"Uneven Culturable Yeast Communities Harbored by Two Sympatric Stingless Bee Species: Scaptotrigona depilis and Tetragona clavipes.","authors":"Rodolfo Bizarria, Ana Ligia Leandrini de Oliveira, Bárbara Gimenes de Castro, Mônica Tallarico Pupo","doi":"10.1002/yea.70048","DOIUrl":"https://doi.org/10.1002/yea.70048","url":null,"abstract":"<p><p>Insects are essential partners for yeasts, playing a key role in their dispersal to new environments. Stingless bees (Apidae: Corbiculata: Meliponini) are social corbiculate bees which lack a functional sting. They are important pollinators in tropical and subtropical regions, foraging for nectar, pollen, and resins, thereby enriching their nests with plant-associated microbes, including yeasts. Here we assessed the yeast diversity associated with stingless bees and their provisions, by thoroughly sampling guts, brood cells, pollen, and honey pots, across seven different Meliponini species: Frieseomelitta varia, Melipona quadrifasciata, Scaptotrigona depilis, Scaptotrigona postica, Tetragona clavipes, Tetragonisca angustula, and Trigona cilipes. Yeast diversity was analyzed for the most extensively sampled bees, S. depilis and T. clavipes. Among the 20 yeast and yeast-like species recovered, we found a core dominant yeast community, primarily composed of Starmerella and Wickerhamiella yeasts, which are notorious to be associated with flowers and floricolous insects. Although the communities are dominated by specific yeast taxa with similar metabolic profiles, it differs among stingless bee species that occur in the same geographic area, as observed for S. depilis and T. clavipes. We suggest that yeast communities associated with stingless bees and their provisions contain overrepresented taxa and are shaped by host foraging choices.</p>","PeriodicalId":23870,"journal":{"name":"Yeast","volume":" ","pages":""},"PeriodicalIF":2.2,"publicationDate":"2026-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149138526","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Using Whole Genome Sequencing to Decipher the Metschnikowia pulcherrima Complex.","authors":"El Khoury Samer, Grondin Cecile, Devillers Hugo, Groenewald Marizeth, Legras Jean-Luc","doi":"10.1002/yea.70047","DOIUrl":"https://doi.org/10.1002/yea.70047","url":null,"abstract":"<p><p>Metschnikowia pulcherrima, an ascomycetous yeast, has been frequently isolated from various substrates associated with agriculture such as nutrient-rich plant materials (fruit, fruit juices and fermenting grape must), soil and with various insects including fruit flies. Due to the growing consumer demand for wines with reduced levels of additives such as sulphites, there has been great interest in this yeast species for its ability to compete with other fungi. Despite having similar phenotypes, many species related to M. pulcherrima have been described in the last 20 years based on the sequences of the LSU D1/D2 domain. These species were grouped together as M. pulcherrima based on primary and secondary barcode sequences, physiological tests and hybridization experiments (Sipiczki 2022a). However, this new classification is supported by limited genetic information. In this project we performed a genomic characterization of 63 strains, including 10 type strains of current synonyms. The combination of different genomic-scale analyses (ANI, k-mer, Busco genes, whole-genome genotyping) conclusively demonstrated the existence of a main cluster of strains representing the M. pulcherrima species and a small sister cluster related to M. chrysoperlae that appeared as potential triploid hybrids. We also observed a high heterozygosity in M. pulcherrima which was significantly higher than S. cerevisiae and the presence of some homozygous strains. Strong linkage disequilibrium decay also suggests active outcrossing leading to recombination in this species. This demonstrates the power of phylogenomic in resolving taxonomic issues and the ability of such approaches to provide information on the life cycle of this species.</p>","PeriodicalId":23870,"journal":{"name":"Yeast","volume":" ","pages":""},"PeriodicalIF":2.2,"publicationDate":"2026-10-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149115570","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Taxogenomic Characterization and Comparative Genomic Analysis of Hermanozyma buritii sp. nov., f.a., a Yeast Species Isolated From Plants and Soil.","authors":"Vitória Petra de O Barros, Ana Raquel O Santos, Júlio Ricardo M Silva, Paula B Morais, Thiago M Batista, Daniela L Souza, Rosangela Santa-Brígida, Marlúcia B Martins, Glória R Franco, Marc-André Lachance, Carlos A Rosa, Melissa Fontes Landell","doi":"10.1002/yea.70049","DOIUrl":"https://doi.org/10.1002/yea.70049","url":null,"abstract":"<p><p>Four yeast isolates with an affinity to Hermanozyma ubatubensis and Hermanozyma aechmeae (Ascomycota, Saccharomycotina, and Metschnikowiaceae) were obtained from plant and soil samples collected at different localities in Brazil. Analyses of the D1/D2 domains of the LSU rDNA gene and phylogenomic data indicate that these isolates represent a new species within the Herm. ubatubensis clade. We propose the name Hermanozyma buritii sp. nov., f.a. to accommodate these isolates. The holotype is CBS 19561. The MycoBank number is MB864970. To further characterize this species, genome sequence data were analyzed for genes involved in carbon and nitrogen metabolism and compared with those of Herm. ubatubensis, Herm. aechmeae, and other phylogenetically related yeast species with publicly available genomes from the genera Australozyma, Helenozyma, Metschnikowia, and Tanozyma. Although sporulation and other evidence of sexual reproduction were not observed, genome analyses revealed the presence of the MATα locus in the strain examined. Herm. buritii exhibited the highest diversity of mating pheromone genes among the analyzed species, with five α-pheromone copies (two variants) and seven mfa loci encoding three distinct peptides, suggesting a potentially versatile mating system. Herm. buritii produced a red-maroon pigment when cultivated in the presence of iron, consistent with pulcherrimin formation through the binding of pulcherriminic acid to ferric iron (Fe³⁺). In addition, all four genes required for pulcherriminic acid biosynthesis were identified in its genome. The phenotypic characteristics of the novel species were compared with those of Herm. ubatubensis and Herm. aechmeae.</p>","PeriodicalId":23870,"journal":{"name":"Yeast","volume":" ","pages":""},"PeriodicalIF":2.2,"publicationDate":"2026-10-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149115578","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Screening of Brazilian Isolated Yeasts Reveals Multiple Potential Chassis for Industrial Biotechnology.","authors":"Giovanna R Maklouf, Lara I S Sousa, Juliana Pimentel Galhardo, Cecilia Trivellin, João Pedro R Prado, Tássia Cristina da Silva, Ana C S R de Carvalho, Juliana José, Gonçalo A G Pereira, Marcelo F Carazzolle, Fellipe S B de Mello","doi":"10.1002/yea.70045","DOIUrl":"10.1002/yea.70045","url":null,"abstract":"<p><p>The future of sustainable industrial biotechnology relies on microbial hosts that can thrive where conventional strains fail. Although Saccharomyces cerevisiae remains the dominant industrial yeast, its metabolic constraints and stress sensitivity highlight the need for alternative microbial chassis. Environmental selection shapes microbial phenotypes, and ecologically diverse isolates represent an underexplored reservoir of industrially relevant traits. Here, we established the Brazilian Yeast Collection (BRYC), comprising 79 isolates spanning 18 genera and 28 named species collected across Brazilian biomes, and systematically characterized their phenotypic diversity under 19 industrially relevant conditions-including carbon sources, biomass-derived inhibitors, elevated temperatures, and high ethanol concentrations-as well as performance on biomass feedstock for industrial bioenergy applications. Across 19 conditions, an industrial S. cerevisiae control ranked first in only one (14% ethanol) despite the panel including strains selected or engineered for low pH tolerance, aldehyde tolerance, xylose utilization, and industrial fermentation performance. 68% of BRYC isolates also exceeded the industrial controls in growth rate robustness. Twenty isolates ranked in the top 10 across three or more conditions, with Wickerhamomyces anomalus (BRYC03 and BRYC30), Pichia kudriavzevii (BRYC98), and Sungouiella flosculorum (BRYC21) emerging as the most versatile performers. Stress-tolerance profiles predicted performance on complex industrial substrates, supporting condition-relevant phenotyping as a framework for rational strain selection. These rankings were, however, substantially format-dependent, with low concordance between liquid and solid cultivation across most conditions (median top-10 overlap = 0.20), underscoring the importance of matching cultivation format to target industrial processes. These findings expand the portfolio of stress-resilient, metabolically versatile yeasts and provide a foundation for their development as next-generation industrial fermenters.</p>","PeriodicalId":23870,"journal":{"name":"Yeast","volume":" ","pages":""},"PeriodicalIF":2.2,"publicationDate":"2026-09-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149040977","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Yeast Knockout Strain Collection: Driving Functional Genomics, Biotechnological Applications and Human Disease Research.","authors":"Takashi Hirasawa","doi":"10.1002/yea.70044","DOIUrl":"https://doi.org/10.1002/yea.70044","url":null,"abstract":"<p><p>The knockout strain collection of Saccharomyces cerevisiae has served as a valuable resource for functional genomics and yeast-based biotechnology studies. A comprehensive single-gene knockout strain collection, covering nearly all non-essential genes, together with complementary mutant collections for essential genes, including temperature-sensitive and transcriptionally regulated strains, has enabled systematic genome-wide analyses of gene function. These resources have facilitated extensive studies of genetic interaction networks, cellular stress responses, metabolic regulation, and adaptive evolution. The yeast knockout strain collection has also been widely utilized in biotechnological research including the identification of genes affecting tolerance to toxic compounds, improvement of metabolite production, and design of synthetic microbial communities, and human disease research such as identification of disease-related genes and treatment targets. This review summarizes the construction and characteristics of the yeast knockout strain collection, key technologies for their application, and recent representative studies in fundamental, biotechnological and human disease research. Furthermore, important considerations for the reliable utilization of the yeast knockout strain collection, including genome instability, neighboring gene effects, and the influence of genetic background, are discussed. Finally, future prospects for applications of the yeast knockout strain collection are outlined.</p>","PeriodicalId":23870,"journal":{"name":"Yeast","volume":" ","pages":""},"PeriodicalIF":2.2,"publicationDate":"2026-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148991579","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative Genomics and Heterologous Complementation Reveal Sequence and Structural Divergence of Selected Transcription Factors From Spathaspora passalidarum.","authors":"Fernanda Matias Albuini, Alex Gazolla de Castro, Lílian Emídio Ribeiro, Lizandra Cristina de Oliveira Figueiredo Gazolla, Carlos Augusto Rosa, Tiago Antônio de Oliveira Mendes, Luciano Gomes Fietto","doi":"10.1002/yea.70046","DOIUrl":"https://doi.org/10.1002/yea.70046","url":null,"abstract":"<p><p>The yeast Spathaspora passalidarum is considered promising for domestication and use in second-generation ethanol production because it ferments xylose and glucose with yields above 70%. However, successful domestication requires not only efficient sugar utilization but also enhanced tolerance to the stresses associated with industrial fermentation, particularly ethanol accumulation, osmotic pressure, and oxidative conditions. To investigate stress responses in Sp. passalidarum, its repertoire of transcription factors and over 300 stress-related proteins were predicted and compared to orthologs in Saccharomyces cerevisiae. While most stress-related proteins were conserved between the two species, more than 70% of Sp. passalidarum transcription factors showed less than 30% sequence identity, lacked recognizable domains, or diverged within functional domains. Gene complementation assays showed that the Sp. passalidarum YAP1 and MSN-like genes showed limited or incomplete complementation of oxidative stress tolerance in S. cerevisiae mutant strains compared with the corresponding native genes. Together, these findings support functional divergence of the analyzed transcription factors in the heterologous S. cerevisiae system and suggest that regulatory divergence may contribute to stress-response differences between Spathaspora passalidarum and Saccharomyces cerevisiae.</p>","PeriodicalId":23870,"journal":{"name":"Yeast","volume":" ","pages":""},"PeriodicalIF":2.2,"publicationDate":"2026-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148991582","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"CryoBN-PAGE: A Rapid and Cost-Effective Method for the Analysis of Yeast Respiratory Complexes and Their Dynamic Organization.","authors":"Lizeth J Camacho Lopez, Mazzen H Eldeeb, Elliana G Mohrhardt, Flavia Fontanesi","doi":"10.1002/yea.70043","DOIUrl":"https://doi.org/10.1002/yea.70043","url":null,"abstract":"<p><p>Over the last three decades, blue native polyacrylamide gel electrophoresis (BN-PAGE), a technique that allows the resolution of large protein complexes in their native conformations, has profoundly impacted the study of mitochondrial biology and our understanding of oxidative phosphorylation (OXPHOS) system's biogenesis and organization. However, while protein samples for BN-PAGE can be prepared from permeabilized mammalian cells, the analysis of yeast OXPHOS complexes requires the prior isolation of mitochondria from whole yeast cells. This requirement limits high-throughput studies and prevents short time-course analyses. We therefore combined BN-PAGE techniques with the cryogenic milling of snap-frozen cells to develop cryoBN-PAGE, which allows for the preparation of BN-PAGE samples from whole-cell yeast lysates and avoids the cost and time associated with mitochondrial isolation. Here, we show the optimization of the method and demonstrate that it can be efficiently paired with a number of downstream applications, such as immunoblots, second-dimension SDS-PAGE, and in gel enzymatic activity assays. Furthermore, by avoiding the time-consuming mitochondrial isolation, cryoBN-PAGE allows for precise time-course assays and the investigation of rapid changes in the properties of the OXPHOS system in response to alterations in environmental conditions.</p>","PeriodicalId":23870,"journal":{"name":"Yeast","volume":" ","pages":""},"PeriodicalIF":2.2,"publicationDate":"2026-09-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148963850","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genetic Tools in the Nakaseomyces clade for Evolutionary Comparisons of Signal Transduction Pathways.","authors":"Maria Abraham, Christine L Iosue, Dennis D Wykoff","doi":"10.1002/yea.70029","DOIUrl":"10.1002/yea.70029","url":null,"abstract":"<p><p>The genus Nakaseomyces provides four species that are closely related but have different characteristics. For example, N. glabratus (formerly known as Candida glabrata) is a common human pathogen, whereas N. bracarensis and N. nivariensis have been isolated in clinical settings but are not common human pathogens. N. delphensis was isolated from fruit and there is no evidence it is pathogenic. Given the differences, we developed the clade as a molecular genetic system where we could introduce plasmids and assess transcriptional output from cloned promoters. We engineered a CRISPR/Cas9 plasmid that allows for rapid Gibson cloning of gRNAs, generated auxotrophic strains for amino acids and nucleotides, and introduced plasmids into each species. We used promoter-YFP plasmids to determine that while there are differences between the species, each species likely has intact thiamine and phosphate (THI and PHO) signal transduction pathways, and that gene expression in N. glabratus and N. bracarensis is more similar to one another than to the other two species. Finally, we determine that N. glabratus, N. bracarensis, and N. nivariensis persist in a murine macrophage for 24 h, whereas N. delphensis does not. This work describes new molecular tools for genetic manipulation in the Nakaseomyces clade and allows for evolutionary questions to be explored.</p>","PeriodicalId":23870,"journal":{"name":"Yeast","volume":" ","pages":"202-213"},"PeriodicalIF":2.2,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13548925/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148151730","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enhanced Production of Recombinant Thermophilic Xylanase X11P in Ogataea polymorpha via In-Silico Signal Peptide Discovery and Fed-Batch Fermentation.","authors":"Kanokarn Kocharin, Chitwadee Phithakrotchanakoon, Somsak Likhitrattanapisal, Aekkachai Puseenam, Kitisak Sansatchanon, Jedsadakorn Ninrat, Wannaporn Sagasae, Paopit Siriarchawatana, Warasirin Sornlek, Supawadee Ingsriswang, Niran Roongsawang","doi":"10.1002/yea.70032","DOIUrl":"10.1002/yea.70032","url":null,"abstract":"<p><p>Efficient secretion of heterologous proteins is essential for advancing yeast-based bioprocesses, yet signal peptide (SP) optimization in the thermotolerant methylotrophic yeast Ogataea polymorpha remains limited. This study integrates in-silico SP discovery, experimental validation, and bioprocess engineering to enhance secretion of the thermophilic xylanase X11P under sucrose-inducible expression. Genome-wide screening of 5184 O. polymorpha proteins using SignalP, Phobius, DeepLoc, WoLF PSORT, and ProP identified 11 high-confidence SP candidates. Comparative analysis with Komagataella phaffii endogenous proteins guided selection of seven SPs for experimental evaluation. Among these, the novel O. polymorpha α-mating factor-like peptide FUN_005010 exhibited strong secretion-promoting activity, with its prepro-sequence yielding the highest extracellular xylanase levels and outperforming the classical Saccharomyces cerevisiae α-MF. To evaluate industrial applicability, sucrose-based fermentation strategies were systematically optimized in a 5-L bioreactor. Controlled sucrose feeding and balanced C/N ratios were found to be critical for maximizing maltase (MAL) promoter-driven expression. A stepwise increasing sucrose feed combined with induction at 30°C enabled X11P titers up to 770 U/mL, representing a 15-fold improvement over shake-flask cultures. This work demonstrates that the combination of SP evaluation and optimized sucrose-inducible fed-batch operation significantly enhances X11P production in O. polymorpha. The identified FUN_005010-prepro SP and the refined process framework provide valuable tools for developing O. polymorpha as a high-performance industrial expression platform.</p>","PeriodicalId":23870,"journal":{"name":"Yeast","volume":" ","pages":"231-242"},"PeriodicalIF":2.2,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13548932/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148259828","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sugar Metabolisms Altered By Undissociated Forms of Organic Acids Based on the Emergence of [GAR⁺] Cells in Saccharomyces cerevisiae.","authors":"Koichi Tanabe, Atsuhiko Nakata, Mizuki Hosotani, Jun Shima","doi":"10.1002/yea.70031","DOIUrl":"10.1002/yea.70031","url":null,"abstract":"<p><p>The glucose repression system is a mechanism for effective energy acquisition by glucose assimilation in microorganisms. In yeast, Saccharomyces cerevisiae, which is known as a prion-like protein [GAR⁺], is involved in the bypass of glucose repression. It has been reported that the emergence of [GAR⁺] cells was promoted by lactate and acetate. Herein, we examined the promotion of [GAR⁺] emergence by pyruvate (both the end product of glycolysis and a precursor of the synthesis of organic acids), and we compared the results of pyruvate with those of lactate or acetate. Pyruvate, as well as other organic acids, promoted [GAR⁺] emergence in all S. cerevisiae strains tested, but the induction patterns of [GAR⁺] emergence by organic acids differed among the strains. The ability of all three organic acids to induce [GAR⁺] was weakened at pH values greater than the pKa value of each organic acid, indicating that the undissociated forms of organic acids may promote [GAR⁺] emergence.</p>","PeriodicalId":23870,"journal":{"name":"Yeast","volume":" ","pages":"223-230"},"PeriodicalIF":2.2,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13548999/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148200168","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}