Yeast最新文献 - Book学术

Exploiting the Geranylgeranyl-Pyrophosphate-Sensing N-Terminal Domain of HMG-CoA Reductase 2 to Regulate Farnesyl Pyrophosphate Synthase (Erg20p) for Improved Monoterpene Production in Saccharomyces cerevisiae. 利用HMG-CoA还原酶2的香叶基焦磷酸传感n端结构域调控法尼基焦磷酸合成酶（Erg20p）以提高酿酒酵母单萜的产量。

IF 2.2 4区生物学

Yeast Pub Date : 2025-07-21 DOI: 10.1002/yea.4005

Zeyu Lu, Samuel Evans, Liam McDonnell, Naga Chandra Bandari, Yilun Weng, Wanli Jin, Robert Speight, Gerhard Schenk, Christopher B Howard, Claudia E Vickers, Bingyin Peng

{"title":"Exploiting the Geranylgeranyl-Pyrophosphate-Sensing N-Terminal Domain of HMG-CoA Reductase 2 to Regulate Farnesyl Pyrophosphate Synthase (Erg20p) for Improved Monoterpene Production in Saccharomyces cerevisiae.","authors":"Zeyu Lu, Samuel Evans, Liam McDonnell, Naga Chandra Bandari, Yilun Weng, Wanli Jin, Robert Speight, Gerhard Schenk, Christopher B Howard, Claudia E Vickers, Bingyin Peng","doi":"10.1002/yea.4005","DOIUrl":"https://doi.org/10.1002/yea.4005","url":null,"abstract":"Dynamic downregulation of the endogenous farnesyl pyrophosphate (FPP) synthase (Erg20p) is crucial to engineer heterologous monoterpene production in the yeast Saccharomyces cerevisiae. FPP downstream metabolite geranylgeranyl pyrophosphate (GGPP) can induce the degradation of 3-hydroxy-3-methylglutaryl (HMG)-CoA reductase 2 (Hmg2p) through its N-terminal GGPP-sensing endoplasmic reticulum transmembrane domain (Hmg2pN) in S. cerevisiae. Here, we investigate the use of Hmg2pN to regulate Erg20p, aiming to restrict FPP synthesis and redirect metabolic flux to monoterpene production. While using the ERG1 promoter to regulate ERG20 transcription improved monoterpene limonene by ~10-fold, combinatory fusion of Hmg2pN to Erg20p N-terminus further improved limonene production by 40% to 0.52 g L-1 in synthetic minimal media. This approach yielded 0.5 g L-1 geraniol in batch cultivation, comparable to levels achieved using the N-end-rule degron K3K15 or an auxin-inducible degron to regulate Erg20p. In rich complex media, this approach was superior, leading to 2.1 g L-1 geraniol production in semi-fed batch cultivation. In summary, the Hmg2pN domain is an efficient tool to constrain FPP synthesis for improved monoterpene production in S. cerevisiae.","PeriodicalId":23870,"journal":{"name":"Yeast","volume":" ","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-07-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144675935","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cooperative Function of Atg8- and TORC1-Mediated Activities in Yeast. 酵母中Atg8-和torc1介导活性的协同功能。

IF 2.2 4区生物学

Yeast Pub Date : 2025-06-26 DOI: 10.1002/yea.4003

Yumiko Oba, Miyuki Higuchi, Naoka Takahashi, Haruko Katsuta, Naoki Koike, Takashi Ushimaru, Yoko Kimura

{"title":"Cooperative Function of Atg8- and TORC1-Mediated Activities in Yeast.","authors":"Yumiko Oba, Miyuki Higuchi, Naoka Takahashi, Haruko Katsuta, Naoki Koike, Takashi Ushimaru, Yoko Kimura","doi":"10.1002/yea.4003","DOIUrl":"https://doi.org/10.1002/yea.4003","url":null,"abstract":"The target of rapamycin complex 1 (TORC1) protein kinase plays an important role in regulating various cellular activities in response to nutrient availability. In this study, an autophagy-related protein 8 (atg8) mutant of Saccharomyces cerevisiae was highly sensitive to cellular processes in which TORC1 activity was inhibited by rapamycin treatment or by a mutated allele of KOG1 which encodes a subunit of TORC1. Atg8 exhibits both lipidation-dependent and -independent activities, each involving distinct factors. Lipidation of Atg8 is necessary for autophagy and functions with autophagy-related proteins like Atg7, whereas the lipidation-independent activities of Atg8 require Hfl1. The atg7Δhfl1Δ double mutant exhibited defects for the impaired TORC1 activities, suggesting that both lipidation-dependent and -independent functions of Atg8 are required for survival during impaired TORC1 activity. Moreover, atg8Δ and atg7Δhfl1Δ mutants exhibited sensitivity to metal ion Zn2+ during low-dose rapamycin treatment. The results suggest that Atg8-mediated functions and TORC1 signaling events play an important role in cell growth, possibly by maintaining vacuole integrity.","PeriodicalId":23870,"journal":{"name":"Yeast","volume":" ","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-06-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144498189","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

An Investigation of TDA1 Deficiency in Saccharomyces cerevisiae During Diauxic Growth. 酿酒酵母生长过程中TDA1缺乏的研究。

IF 2.2 4区生物学

Yeast Pub Date : 2025-06-01 Epub Date: 2025-06-26 DOI: 10.1002/yea.4004

Erik Y Bjurström, Praphapan Lasin, Daniel Brunnsåker, Ievgeniia A Tiukova, Ross D King

{"title":"An Investigation of TDA1 Deficiency in Saccharomyces cerevisiae During Diauxic Growth.","authors":"Erik Y Bjurström, Praphapan Lasin, Daniel Brunnsåker, Ievgeniia A Tiukova, Ross D King","doi":"10.1002/yea.4004","DOIUrl":"10.1002/yea.4004","url":null,"abstract":"Tda1p is a protein kinase in Saccharomyces cerevisiae. Here we investigate the function of TDA1 during the diauxic shift using transcriptomics. We compared the gene expression in the deletion mutant tda1∆ and the reference strain (BY4741) during both the aerobic fermentation phase (log phase), and the respiratory phase (post-diauxic shift phase, PDS) in three separate independent experiments. We found: Differential gene expression analysis showed that compared to the reference strain, the tda1∆ mutant exhibited an upregulation of the glucose repressed hexose transporter HXT6 during the log phase, and upregulation of mitochondrial proteins and genes related to mitochondrial translation during the PDS phase. Gene set enrichment analysis showed an enrichment in mitochondrial translation in the PDS phase for the deletion mutant tda1∆, but not for the reference strain. Transcription factor analysis showed that the enrichment of Mig1p repressed genes was not statistically significant in TDA1 deletion mutants for neither log-phase nor PDS-phase. This conflicted with the previously suggested model that argued for an interaction between Tda1p and Mig1p. Instead, transcription factor analysis showed an enrichment of genes regulated by the HAP-complex, which regulates mitochondrial translation, during the PDS-phase in the tda1∆ mutant. The combined evidence from this study indicates that Tda1p does not participate in Mig1p-mediated glucose repression. Instead, we propose that it is involved in the regulation of mitochondrial translation by repressing the expression of HAP complex subunits.","PeriodicalId":23870,"journal":{"name":"Yeast","volume":" ","pages":"142-156"},"PeriodicalIF":2.2,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12232523/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144498188","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cytoduction Preserves Genetic Diversity Following Plasmid Transfer Into Pooled Yeast Libraries. 质粒转入酵母文库后，细胞传导保留了遗传多样性。

IF 2.2 4区生物学

Yeast Pub Date : 2025-06-01 Epub Date: 2025-04-07 DOI: 10.1002/yea.4001

Han-Ying Jhuang, Dimitra Aggeli, Gregory I Lang

{"title":"Cytoduction Preserves Genetic Diversity Following Plasmid Transfer Into Pooled Yeast Libraries.","authors":"Han-Ying Jhuang, Dimitra Aggeli, Gregory I Lang","doi":"10.1002/yea.4001","DOIUrl":"10.1002/yea.4001","url":null,"abstract":"Introducing plasmids into yeast is a critical step for many phenotypic assays and genetic engineering applications. However, it is often challenging for applications that involve large pools of variants because the population structure can be easily altered by traditional methods such as chemical transformation. In this study, we introduce drug-marked plasmids into a heterogeneous yeast population using both transformation and cytoduction (mating without nuclear fusion). Using a highly diverse barcoded yeast collection, we quantify the efficiency of both methods. We demonstrate that for cytoduction, but not transformation, nearly all the genotypes in the initial pool were detected in the final pool, with a high correlation to their initial frequencies. Finally, we map QTL that impact both cytoduction and transformation. Overall, we demonstrate the efficiency of cytoduction as a means of introducing plasmids into yeast. This is significant because it provides a means of manipulating diverse yeast populations, such as pools constructed for bulk segregant analysis, deep mutational scanning, large-scale gene editing, or populations from long-term evolution experiments.","PeriodicalId":23870,"journal":{"name":"Yeast","volume":" ","pages":"126-131"},"PeriodicalIF":2.2,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12232603/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143796459","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A Rapid Growth Rate Underpins the Dominance of Hanseniaspora uvarum in Spontaneous Grape Juice Fermentations. 快速的生长速度支持了葡萄芽孢杆菌在葡萄汁自发发酵中的优势地位。

IF 2.2 4区生物学

Yeast Pub Date : 2025-06-01 Epub Date: 2025-03-21 DOI: 10.1002/yea.4000

Cristobal A Onetto, Jane McCarthy, Simon A Schmidt

{"title":"A Rapid Growth Rate Underpins the Dominance of Hanseniaspora uvarum in Spontaneous Grape Juice Fermentations.","authors":"Cristobal A Onetto, Jane McCarthy, Simon A Schmidt","doi":"10.1002/yea.4000","DOIUrl":"10.1002/yea.4000","url":null,"abstract":"Hanseniaspora uvarum is consistently observed as the dominant non-Saccharomyces species in spontaneous grape juice fermentations. However, the physiological mechanisms and physicochemical variables influencing the prevalence of H. uvarum over other non-Saccharomyces species remain unclear. We tested the factors contributing to H. uvarum dominance by inoculating a chemically diverse set of grape juices with a mock community whose composition was based on a previously published comprehensive microbial survey of commercial spontaneous fermentations. The diverse composition of these grape juices appeared to have minimal impact on the overall microbial dynamics of fermentation, with H. uvarum consistently emerging as the dominant non-Saccharomyces species in nearly all conditions tested. Flow cytometry analysis confirmed that H. uvarum has a faster growth rate than Saccharomyces cerevisiae and several other Hanseniaspora species. Moreover, its growth was not affected by the presence of S. cerevisiae. H. uvarum negatively affected the growth of S. cerevisiae, with significant implications for fermentation performance and sugar consumption. Our study suggests that the fast growth rate of H. uvarum enables it to dominate the grape juice environment quickly during early fermentation stages. This physiological advantage may be critical to the outcome of spontaneous fermentations, as evidenced by its direct impact on S. cerevisiae and fermentation performance.","PeriodicalId":23870,"journal":{"name":"Yeast","volume":" ","pages":"116-125"},"PeriodicalIF":2.2,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143674604","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Saccharomycopsis yichangensis sp. nov., a Novel Predacious Yeast Species Isolated From Soil. 宜昌酵母菌（Saccharomycopsis ychangensis sp. nov.）——一种从土壤中分离的新型掠食性酵母。

IF 2.2 4区生物学

Yeast Pub Date : 2025-06-01 Epub Date: 2025-05-20 DOI: 10.1002/yea.4002

Shuang Hu, Liang-Chen Guo, Yan-Jie Qiu, Qi-Yang Zhu, Ri-Peng Zhang, Pei-Jie Han, Feng-Yan Bai

{"title":"Saccharomycopsis yichangensis sp. nov., a Novel Predacious Yeast Species Isolated From Soil.","authors":"Shuang Hu, Liang-Chen Guo, Yan-Jie Qiu, Qi-Yang Zhu, Ri-Peng Zhang, Pei-Jie Han, Feng-Yan Bai","doi":"10.1002/yea.4002","DOIUrl":"10.1002/yea.4002","url":null,"abstract":"Two yeast strains belonging to the ascomycetous yeast genus Saccharomycopsis were isolated from soil collected from a forest in Wufeng Tujia Autonomous County, Yichang, Hubei province, China. Phylogenetic analyzes of the internal transcribed spacer (ITS) region and the D1/D2 domain of the large subunit rRNA gene showed that they closely related to S. fermentans and S. babjevae but differed from S. fermentans by 17 (3.09%, 15 substitutions and two gaps) and 30 (4.85%, 22 substitutions and eight gaps) mismatches, and from S. babjevae by 13 (2.39%, eight substitutions and five gaps) and 21 (3.46%, 14 substitutions and seven gaps) mismatches in the D1/D2 domain and ITS region, respectively. A phylogenomic analysis based on 1260 single-copy orthologs confirmed the close relationship of the new Chinese strains with S. fermentans and S. babjevae. The whole genome average nucleotide identity (ANI) values of the new strains with the two species are 85.7% and 86.9%, respectively. The results suggest that the two strains represent a novel species, for which the name Saccharomycopsis yichangensis sp. nov. (holotype strain CGMCC 2.7390) is proposed. The Fungal Names number is FN 572295. The novel yeast is homothallic and produces asci containing four spheroidal ascospores with an equatorial or subequatorial ledge. This species can prey on cells of Jamesozyma jinghongensis, Meyerozyma carpophila and Saccharomyces cerevisiae through invasive infection pegs.","PeriodicalId":23870,"journal":{"name":"Yeast","volume":" ","pages":"132-141"},"PeriodicalIF":2.2,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144112219","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Enhancing Yeast Transformation: Achieving up to a Tenfold Increase Through a Single Adjustment in the Lithium Acetate-Polyethylene Glycol Method. 增强酵母菌转化：通过在醋酸锂-聚乙二醇方法中的一次调整实现高达十倍的增加。

IF 2.2 4区生物学

Yeast Pub Date : 2025-06-01 Epub Date: 2025-03-15 DOI: 10.1002/yea.3999

Mathilde Kadouch, Pierre Gaspin, Christelle Marchal, Sabine Castano, Christophe Cullin

引用次数: 0

Pro-Oxidant Influence of Quercetin Supplementation in Saccharomyces cerevisiae. 补充槲皮素对酿酒酵母促氧化作用的影响。

IF 2.2 4区生物学

Yeast Pub Date : 2025-04-01 Epub Date: 2025-02-23 DOI: 10.1002/yea.3992

Andres Carrillo-Garmendia, Ana Leticia Vaca-Martinez, Blanca Lucia Carmona-Moreno, Juan Carlos González-Hernández, Jose Angel Granados-Arvizu, Sofia Maria Arvizu-Medrano, Jorge Gracida, Rosa Martha Pérez-Serrano, Gerardo M Nava, Carlos Regalado-Gonzalez, Luis Alberto Madrigal-Perez

引用次数: 0

Effects of Rainfall and Drought on the Functional and Taxonomic Diversity of Cultivable Yeasts Associated With Bromelia laciniosa From a Brazilian Tropical Dryland. 降雨和干旱对巴西热带旱地凤梨相关可栽培酵母功能和分类多样性的影响

IF 2.2 4区生物学

Yeast Pub Date : 2025-04-01 Epub Date: 2025-03-07 DOI: 10.1002/yea.3997

Ciro R Félix, Bruno E S Nascimento, Victor Tavares, Melissa F Landell

{"title":"Effects of Rainfall and Drought on the Functional and Taxonomic Diversity of Cultivable Yeasts Associated With Bromelia laciniosa From a Brazilian Tropical Dryland.","authors":"Ciro R Félix, Bruno E S Nascimento, Victor Tavares, Melissa F Landell","doi":"10.1002/yea.3997","DOIUrl":"10.1002/yea.3997","url":null,"abstract":"The phyllosphere is a crucial interface for plant-environment interactions, hosting a diverse microbial community, including yeasts. This community affects the host's fitness and can act as a plant resilience booster. Nonetheless, abiotic factors can have a significant impact on the microbial community. Therefore, this work aims to investigate the potential effects of rain and drought on the taxonomic and functional diversity of epiphytic yeasts associated with Bromelia laciniosa leaves in the Caatinga, a tropical dryland in South America. A total of 262 isolates were obtained. Based on their D1/D2 region of the LSU gene rRNA sequences, the isolates were identified as belonging to 76 species of yeasts and yeast-like fungi, including 53 Basidiomycetes and 23 Ascomycetes. Furthermore, 23 species (ca. 30% of the total) are possible new species. Most of the variables related to rainfall and drought did not affect the yeast taxonomic diversity. Furthermore, the impact of rain and drought on the community composition differs between functional and taxonomic diversities, which may suggest a decoupling between these dimensions. The functional and taxonomic structure of the yeast community in the Caatinga is complex, and rain and drought alone are not the absolute factors governing its dynamics. Additionally, the functional traits may provide valuable insights into the behavior of the yeast community in bromeliads and help predict the effects of dry-wet cycles on the leaf-inhabiting yeast community, as well as potential impacts on the host.","PeriodicalId":23870,"journal":{"name":"Yeast","volume":" ","pages":"70-84"},"PeriodicalIF":2.2,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143587419","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A Key Role of the EMC Complex for Mitochondrial Respiration and Quiescence in Fission Yeasts. EMC复合体在裂变酵母线粒体呼吸和静止中的关键作用。

IF 2.2 4区生物学

Yeast Pub Date : 2025-04-01 Epub Date: 2025-03-14 DOI: 10.1002/yea.3998

Modesto Berraquero, Víctor A Tallada, Juan Jimenez

引用次数: 0