Yi chuan xue bao = Acta genetica Sinica最新文献_第8页

No association between attention-deficit hyperactivity disorder and catechol-O-methyltransferase gene in Chinese. 中国人注意缺陷多动障碍与儿茶酚- o -甲基转移酶基因无相关性。

Yi chuan xue bao = Acta genetica Sinica Pub Date : 2005-08-01

San-Duo Jiang, Xiao-Dong Wu, Ye Zhang, Guo-Mei Tang, Yi-Ping Qian, Dong-Xiang Wang

引用次数: 0

[Construction of RNA-containing virus-like nanoparticles expression vector with cysteine residues on surface and fluorescent decoration]. [表面有半胱氨酸残基的含rna病毒样纳米颗粒表达载体的构建及荧光修饰]。

Yi chuan xue bao = Acta genetica Sinica Pub Date : 2005-08-01

Yang-Jian Cheng, Ji-Xuan Liang, Qing-Ge Li

{"title":"[Construction of RNA-containing virus-like nanoparticles expression vector with cysteine residues on surface and fluorescent decoration].","authors":"Yang-Jian Cheng, Ji-Xuan Liang, Qing-Ge Li","doi":"","DOIUrl":"","url":null,"abstract":"Site-directed mutagenesis was performed at the codon 15 of the MS2 bacteriophage coat protein gene,which had been cloned to the virus-like particles expression vector containing non-self RNA fragment. The produced expression vector,termed pARSC, was transformed to E. coli DH5alpha. The positive clones were selected and proliferated. The harvested cells were treated with sonication and the supernatant was then subjected to linear sucrose density gradients centrifugation (15% to 60%) at 32000 r/min for 4 h at 4 degrees C. The virus-like particles, VLP-Cy, were collected at 35% sucrose density. The particles were examined by transmission electron microscopy and the spherical viral particles of approximately 27 nm in diameter were found. The thiolated VLP-Cy was then chemically modified with fluorescein -5'-maleimide. The covalent fluorescent labeling was confirmed by absorption analysis, SDS-PAGE and MALDI-TOF mass spectroscopy. This is the first report of preparation of RNA-containing natural fluorescent nanoparticles. The study highlight the versatility of MS2 bacteriophage capsids as building blocks for functional nanomaterials construction for a variety of application purposes.","PeriodicalId":23770,"journal":{"name":"Yi chuan xue bao = Acta genetica Sinica","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2005-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25653558","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[QTL analysis for traits associated with photosynthetic functions in rice (Oryza sativa L.)]. 水稻光合功能相关性状的QTL分析[j]。

Yi chuan xue bao = Acta genetica Sinica Pub Date : 2005-08-01

Mao-Long Hu, Chun-Ming Wang, Quan-Hai Yang, Hu-Qu Zhai, Wei Lu, Rong-Xian Zhang, Jian-Min Wan

{"title":"[QTL analysis for traits associated with photosynthetic functions in rice (Oryza sativa L.)].","authors":"Mao-Long Hu, Chun-Ming Wang, Quan-Hai Yang, Hu-Qu Zhai, Wei Lu, Rong-Xian Zhang, Jian-Min Wan","doi":"","DOIUrl":"","url":null,"abstract":"A mapping population of 81 F11 lines (Recombinant Inbred Lines, RILs), derived from a cross between a japonica variety Kinmaze and an indica variety DV85 by single-seed descent method, was used to detect quantitative trait loci (QTL) for traits associated with photosynthetic functions. Total leaf nitrogen content (TLN), chlorophyll a/b ratio (Chl. a:b) and chlorophyll content (Chl) were measured in leaves of rice (Oryza sativa L.) at the 7th day after heading. A total of six putative QTLs were detected with percentage of variance explained (PVE) ranging between 11.2% -29.6%, and LOD of QTLs 2.66-4.81. Of those putative QTLs, three for TLN were detected on chromosomes 1,2 and 11, with PVE of 17.3%, 15.3% and 13.7%, respectively;Two controlling Chl. a:b on chromosomes 3 and 4, PVE of 13.8% and 29.6%, one for Chl on chromosome 1, PVE of 11.2%. Four of those detected QTLs were newly reported in this study. Interestingly, the QTL controlling chlorophyll content,namely qCC-1 reported here,was detected in the region of the RFLP marker C122 on chromosome 1, where harbored NADH-glutamate synthase structure gene according to a previous study. Because the biosynthesis of chlorophyll begins with glutamate, qCC-1 would play a vital role in photosynthetic functions. Whereas,no QTL controlling chlorophyll content were detected at the 30th day after heading, suggesting that the effect of the QTL controlling chlorophyll content decreased during leaf senescence.","PeriodicalId":23770,"journal":{"name":"Yi chuan xue bao = Acta genetica Sinica","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2005-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25662339","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Inheritance analysis and molecular marker selection of genes for wheat spindle streak mosaic disease resistance]. 小麦轴条花叶病抗性基因的遗传分析和分子标记选择。

Yi chuan xue bao = Acta genetica Sinica Pub Date : 2005-07-01

Qing-Ping Zhang, Xiu-E Wang, Yao-Nan Wang, Yan Zhao, Hai-Yan Wang, Su-Ling Wang, Pei-Du Chen

{"title":"[Inheritance analysis and molecular marker selection of genes for wheat spindle streak mosaic disease resistance].","authors":"Qing-Ping Zhang, Xiu-E Wang, Yao-Nan Wang, Yan Zhao, Hai-Yan Wang, Su-Ling Wang, Pei-Du Chen","doi":"","DOIUrl":"","url":null,"abstract":"Three wheat spindle streak mosaic viruses (WSSMV) resistant cultivars ('Yining Xiaomai', 'Xu87-633', and 'Xifeng') and one susceptible cultivar ('Zhen9523') were used as parents of 3 crosses in this experiment. WSSMV resistance of the parents, F1, and F2 was evaluated under field condition. Based on the segregation ratios of resistant and susceptible plants in F, and F2 populations, it was deduced that the resistance to WSSMV was dominant and the inheritable factors controlling WSSMV resistance were encoded by the nuclear genome. WSSMV resistances in 'Yining Xiaomai' and 'Xifeng' were controlled by two pairs of alleles, which showed complementary effects. However the resistance in 'Xu-87633' was controlled by a single dominant gene. 266 pairs of SSR primers located on 21 wheat chromosomes were used for polymorphic analysis of the two resistant and the susceptible parents 'Yining Xiaomai' and 'Zhen9523', and 108 of them amplified polymorphic DNA products. By Bulk Segregant Analysis of resistant and susceptible pools, one pair of primer located on chromosome arm 2DS, Xgwm261, were found being linked to WSSMV resistance. The 224 F2 individuals were then amplified with marker Xgwm261. The statistic genetic distance between Xgwm261 and the resistance locus was calculated to be 22.9 cM using the software Mapmaker 3.0.","PeriodicalId":23770,"journal":{"name":"Yi chuan xue bao = Acta genetica Sinica","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2005-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25229003","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Refinement of DSAP1 locus and mutation detection for candidate genes]. [DSAP1基因座的改良及候选基因的突变检测]。

Yi chuan xue bao = Acta genetica Sinica Pub Date : 2005-07-01

Zheng-Hu Zhang, Zhen-Min Niu, Wen-Tao Yuan, Jing-Jun Zhao, Fa-Xing Jiang, Jing Zhang, Bao Chai, Xiao-Yan Xiong, Lei-Hong Xiang, Yi Wang, Shi-Jie Xu, Wei-Da Liu, Zhi-Zhong Zheng, Wei Huang

{"title":"[Refinement of DSAP1 locus and mutation detection for candidate genes].","authors":"Zheng-Hu Zhang, Zhen-Min Niu, Wen-Tao Yuan, Jing-Jun Zhao, Fa-Xing Jiang, Jing Zhang, Bao Chai, Xiao-Yan Xiong, Lei-Hong Xiang, Yi Wang, Shi-Jie Xu, Wei-Da Liu, Zhi-Zhong Zheng, Wei Huang","doi":"","DOIUrl":"","url":null,"abstract":"Disseminated superficial actinic porokeratosis (DSAP) is an uncommon autosomal dominant chronic keratinization disorder,characterized by multiple superficial keratotic lesions surrounded by a slightly raised keratotic border. In previous studies,the disease gene was mapped to 12q23. 2-24.1 (DSAP1), and 15q25. 1-26.1 (DSAP2). In this study,genome-wide scan was performed in two unrelated six-generation DSAP pedigrees to localize and identify the candidate gene(s) of disease. Linkage analysis showed that the cumulative maximum two-point lod score of 8.28 was obtained with the marker D12S84 at a recombination fraction theta of 0.00. Haplotype analysis defined an 8.0 cM critical region for DSAP gene(s) between markers D12S330 and D12S354 on 12q24. 1-q24. 2, which partially overlapped with the region identified for DSAP1. DNA sequencing of the coding exons of six candidate genes (CRY1, PWP1, ASCL4, PRDM4, KIAA0789 and CMKLR1) on the basis of their location in the critical overlap interval, failed to detect any mutation in DSAP patients. Thus, it is likely that these genes are not involved in DSAP.","PeriodicalId":23770,"journal":{"name":"Yi chuan xue bao = Acta genetica Sinica","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2005-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25229646","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Characterization of the beta-actin gene of the rice field eel and its phylogeny in fish]. 稻田鳗β -肌动蛋白基因的鉴定及其在鱼类中的系统发育。

Yi chuan xue bao = Acta genetica Sinica Pub Date : 2005-07-01

Lai-Xin Xia, Han-Hua Cheng, Yi-Qing Guo, Rong-Jia Zhou

引用次数: 0

[Genetic analysis and molecular markers of a novel stripe rust resistance gene YrHua in wheat originated from Psathyrostachys huashanica Keng]. 小麦抗条锈病新基因YrHua的遗传分析及分子标记[j]。

Yi chuan xue bao = Acta genetica Sinica Pub Date : 2005-07-01

Zhang-Jun Cao, Xian-Ping Wang, Mei-Nan Wang, Shuang-He Cao, Jin-Xue Jing, Hong-Sheng Shang, Zhen-Qi Li, Xiang-Qi Zhang

{"title":"[Genetic analysis and molecular markers of a novel stripe rust resistance gene YrHua in wheat originated from Psathyrostachys huashanica Keng].","authors":"Zhang-Jun Cao, Xian-Ping Wang, Mei-Nan Wang, Shuang-He Cao, Jin-Xue Jing, Hong-Sheng Shang, Zhen-Qi Li, Xiang-Qi Zhang","doi":"","DOIUrl":"","url":null,"abstract":"The H9020-17-5,a common wheat-Psathyrostachys huashanica Keng translocation line, possesses excellent resistance to wheat stripe rust. Genetic analysis of F2 and BC1 populations derived from H9020-17-5 x Mingxian169 indicated that resistance to stripe rust in H9020-17-5 was a dominant character controlling by single gene originated from Ps. huashanica. This resistance gene originated from Ps. huashanica was first reported in the present study and named as YrHua. In order to map the resistance gene YrHua, AFLP approach was employed to analyze the 119 individuals of H9020-17-5 x Mingxian169 F2 population which were inoculated by stripe rust isolate CY30. As a result,two markers, PM14(301) and PM42(249) were found to be linked to the resistance gene YrHua,and the genetic distances between the markers and target gene were 5.4 cM and 2.7 cM, respectively. For the convenience of marker-assisted selection in wheat breeding, one of the two AFLP markers was converted to PCR marker using a pair of special primers based on the DNA sequence of PM14 (301) and the polymorphism of restriction site. Our research results provided a useful tool for marker-assisted selection and laid a foundation of fine mapping and map based cloning of YrHua gene.","PeriodicalId":23770,"journal":{"name":"Yi chuan xue bao = Acta genetica Sinica","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2005-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25229004","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Association analysis between polymorphisms of PON gene cluster with coronary heart disease in Chinese]. 中国人PON基因簇多态性与冠心病的相关性分析

Yi chuan xue bao = Acta genetica Sinica Pub Date : 2005-07-01

Xiao-Ling Wang, Zhong-Jie Fan, Jian-Feng Huang, Shao-Yong Su, Jian-Gong Zhao, Dong-Feng Gu

{"title":"[Association analysis between polymorphisms of PON gene cluster with coronary heart disease in Chinese].","authors":"Xiao-Ling Wang, Zhong-Jie Fan, Jian-Feng Huang, Shao-Yong Su, Jian-Gong Zhao, Dong-Feng Gu","doi":"","DOIUrl":"","url":null,"abstract":"An extensive association analysis of PON gene cluster (PONs) with coronary heart disease (CHD) was performed in Chinese Han population. Eleven polymorphisms of PON1, PON2 and PON3 gene were investigated for association with CHD in 474 male patients and 475 controls. Univariate analyses showed the cases had significantly higher frequencies of PON1 192Q allele, 160R allele, -162A allele and PON2 311C allele than were seen in the controls. Logistic regression analyses revealed only the PON1 R160G and -162G/A polymorphisms remained significantly associated with CHD (P = 0.0054 and P = 0.0002). Haplotype analyses for various polymorphism combinations further confirmed the results of individual polymorphism analyses. Only the frequencies of haplotypes containing -162A allele were significantly higher,whereas only the frequencies of haplotypes containing 160G allele significantly lower in cases than those in controls in various polymorphism combinations. This extensive association study has identified the PON1 -162G/A and R160G polymorphisms to be independently associated with CHD in Chinese Han population,and warrants further study to elucidate the biological mechanism.","PeriodicalId":23770,"journal":{"name":"Yi chuan xue bao = Acta genetica Sinica","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2005-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25229647","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Locating maize male sterility gene induced by space flight using microsatellite markers]. [利用微卫星标记定位航天诱导玉米雄性不育基因]。

Yi chuan xue bao = Acta genetica Sinica Pub Date : 2005-07-01

Fu-Xia Liu, Mo-Ju Cao, Ting-Zhao Rong, Guang-Tang Pan

引用次数: 0

[Molecular cloning and expression of an isotoxin gene, alpha-bungarotoxin, from Bungarus multicinctus]. 一种异毒素基因的分子克隆和表达[a-bungarotoxin]。

Yi chuan xue bao = Acta genetica Sinica Pub Date : 2005-07-01

Fang Wang, Yi-Quan Wang

引用次数: 0