Yi chuan xue bao = Acta genetica Sinica最新文献

{"title":"[Effect of population size of performance test on short-term selection result of sire line].","authors":"Hao Zhang,&nbsp;Jia-Qi Li,&nbsp;Chong Wang,&nbsp;Xiao-Hong Liu,&nbsp;Yao-Sheng Chen","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The effects of the population size,boar to sow ratio,and the number of pigs tested per litter on the selection response and its coefficient of variation, inbreeding coefficient of sire line selection were studied. Pig populations of 100 to 500 sows were simulated with Monte Carlo method,and the ratios of boar to sow were 1:10 and 1:20. The number of pigs tested per litter were 2 and 4 composed equally of boars and gilts. The traits selected were average daily gain after weaning (ADG) and backfat thickness (BF) at 100 kg live weight. Breeding pigs were selected according to multiple-trait BLUP,and economic weight of BF was set as 2.5 times of that of ADG. After five generation selection, the results showed that the more the number of sows of the breeding population, the higher the cumulative selection response at 5th generation, and the slower the coefficient of inbreeding increment per generation, the smaller the coefficient of variation of the cumulative selection response at 5th generation. Increasing the number of pigs tested per litter and/or the boar to sow ratio increased the cumulative selection response at 5th generation, inbreeding coefficient increment per generation, and the coefficient of variation of the cumulative selection response at 5th generation. The cumulative selection response got higher and the increment of inbreeding coefficient slowed down significantly when the number of sows in the breeding population increased from 100 to 300,and the ratio of boar to sow and the number of pigs tested per litter were fixed. When the sow number of breeding population was increased from 300 to 400 above,the cumulative selection response only increased slightly,and the coefficient of inbreeding continued to decrease but at a diminished rate. In conclusion, for the short-term selection of sire line, it is recommended that the breeding population should be composed of 400 or above sows, 4 pigs tested per litter,and the ratio of boar to sow should be maintained at 1:20.</p>","PeriodicalId":23770,"journal":{"name":"Yi chuan xue bao = Acta genetica Sinica","volume":"32 7","pages":"696-703"},"PeriodicalIF":0.0,"publicationDate":"2005-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25229650","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Molecular basis of familial hypercholesterolemia-like phenotype heterogeneity].","authors":"Lü-Ya Wang,&nbsp;Jie Lin,&nbsp;Shu Liu,&nbsp;Bao-Sheng Chen","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Familial hypercholesterolemia (FH),which is caused by low-density lipoprotein (LDL) receptor mutation, leads to LDL-R dysfunction and high plasma LDL level and early onset of cardiovascular disease. LDL-R mutation has been regarded as the only cause of FH phenotype. However, evidences from recent studies showed that another six gene mutations can also result in FH like phenotype through different mechanism. Further studies on these genes will clarify the mechanism of plasma LDL regulation and provide the molecular basis for the diagnosis and treatment of patients with FH-like phenotype. This review summarizes recent studies on the molecular basis of FH-like phenotype heterogeneity in the hope of drawing more attention to the disease.</p>","PeriodicalId":23770,"journal":{"name":"Yi chuan xue bao = Acta genetica Sinica","volume":"32 7","pages":"770-7"},"PeriodicalIF":0.0,"publicationDate":"2005-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25230066","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Bioinformatic analysis of the 14-3-3 gene family in rice].","authors":"Gu-Lei Jin,&nbsp;Xu-Sheng Wang,&nbsp;Jun Zhu","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Using two-step HMM (hidden markov model) scan strategy,eight 14-3-3-like proteins were identified by searching the Oryza sativa L. ssp. japonica protein database. From them four genes were newly detected in this study. We confined the genes expressing in Nipponbare by EST search. Expression analysis also showed each gene expressed diversely within any individual,this tends suggested specific function of particular gene. Alignment of amino acid sequences suggested that there could be isoform function of the specific residues. The analyses of gene structure and chromosome location indicated that rice genome contains both epsilon and no-epsilon forms of 14-3-3 proteins. In addition,we analyzed the evolution of the rice 14-3-3 protein family.</p>","PeriodicalId":23770,"journal":{"name":"Yi chuan xue bao = Acta genetica Sinica","volume":"32 7","pages":"726-32"},"PeriodicalIF":0.0,"publicationDate":"2005-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25229002","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Expression of two plant agglutinin genes in transgenic tobacco plants].","authors":"Zhao-Hua Liu, Zhen-Shan Zhang, Hong-Nian Guo, Yan-Tao Jia, Guang-Yu Zheng, Ying-Chuan Tian","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>A plant expression vector pBACG containing the DNA sequence coding for Amaranthus caudatus agglutinin (ACA) and a modified Glanthus nivalis agglutinin (GNA) gene was constructed. Leaf explants of Nicotiana tobacum cv. SRI were transformed with A. tumefaciens LBA4404 harbouring the above expression vector. Results from PCR and Southern blotting analysis showed that both the ACA and GNA gene were inserted into the genome of transformed tobacco plants. Western blottingting analysis of soluble protein isolated from transgenic plants showed that ACA and GNA were synthesized. The results from insect bioassay with peach aphids ( Myzus persicae) revealed that the transgenic plants of pBACG had acquired high resistance against peach aphids. The average aphid-inhibition rate reached up to 83.9% and 85.3% for transgenic plants (T0) and their selfed progenies (T1) respectively,indicating that the functions of these two genes were inheritable.</p>","PeriodicalId":23770,"journal":{"name":"Yi chuan xue bao = Acta genetica Sinica","volume":"32 7","pages":"758-63"},"PeriodicalIF":0.0,"publicationDate":"2005-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25230064","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Cloning of ACC oxidase gene and inhibition of endogenous gene expression with RNAi in cauliflower].","authors":"Yin-Hua Chen,&nbsp;Jun-Hong Zhang,&nbsp;Bo Ouyang,&nbsp;Han-Xia Li,&nbsp;Zhi-Biao Ye","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>A fragment of 1202 bp of the candidate ACO gene was amplified from the cauliflower (brassica oleracea Var. botrytis) genome using the degenerated primers which were designed according to the consensus sequence of ACO amino acids among various plant species. The result of BLAST showed the sequence presented a very high match with the ACO genes from other plants; the homologue was from 83% to 99%. Three exons and two introns were identified in this sequence. The spliced length of mRNA was 756 nt and encoded 252 amino acids. The putative new gene was denominated BoACO, and submitted to GenBank (AY676466). Using the sequence, we constructed an RNA interference (RNAi) transformation vector through the way of BP cloning. The transformation into cauliflower was performed. Five regenerated plants with kanamycin resistance were obtained. And the transgene integrated into cauliflower genome was proved with PCR and Southern blotting. The expression of this ACO gene is down-regulated based on the Northern blotting in the transgenic plants. The activity of ACO enzyme was depressed significantly.</p>","PeriodicalId":23770,"journal":{"name":"Yi chuan xue bao = Acta genetica Sinica","volume":"32 7","pages":"764-9"},"PeriodicalIF":0.0,"publicationDate":"2005-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25230065","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Cloning and analysis of a new NBS-LRR resistance gene family in rice].","authors":"Shi-Quan Wang,&nbsp;De-Chun Zhang,&nbsp;Ping Li,&nbsp;Xu-Dong Wang,&nbsp;Shi-Gui Li,&nbsp;Li-Huang Zhu,&nbsp;Wen-Xue Zhai","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Sequence-based gene isolation has been a practical approach for plant resistance gene cloning. In this study, RS13, a cloned rice sequence with the NBS (nucleotide-binding site) domain of resistance genes, was used as a probe to screen a bacterial artificial chromosome (BAC) library of rice variety IR64,and four positive clones were obtained. Of them the clone 14E19 covered the other three clones and was sequenced through a shotgun approach. The whole sequence of the insert fragment of 14E19 was assembled into approximately 73 kb in length. Genes on the whole assembled sequence were predicted,and four genes encoding NBS and LRR (leucine-rich repeat) domains were found, named as NL-A, B, C and D respectively. For further analysis, another longer BAC clone,106P13, covering 14E19 on the same chromosome position was identified from a BAC library of IRBB56 which had the same genome background with IR64. Ten NL-homologous copies were discovered on the sequence of the BAC clone 106P13, and four copies were identical with those on 14E19. The similar homologous sequences were also found in the genomic sequences of Nipponbare,93-11 and Guangluai4. However, NL sequences were less homologous with the known NBS-LRR resistance genes. This result indicated that NL was a new NBS-LRR gene family and was composed of ten members at least. RT-PCR and cDNA screening displayed that NL-B expressed in a bacterial blight-resistant rice variety IRBB4, indicating the gene was possibly involved in resistance reactions.</p>","PeriodicalId":23770,"journal":{"name":"Yi chuan xue bao = Acta genetica Sinica","volume":"32 7","pages":"704-11"},"PeriodicalIF":0.0,"publicationDate":"2005-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25228999","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[QTL mapping and interaction analysis of genotype x environment (Fe2+ -concentrations ) for mesocotyl length in rice (Oryza sativa L.)].","authors":"You-Nan Ouyang,&nbsp;Qiu-Ying Zhang,&nbsp;Ke-Qin Zhang,&nbsp;Sheng-Miao Yu,&nbsp;Jie-Yun Zhuang,&nbsp;Qian-Yu Jin,&nbsp;Shi-Hua Cheng","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>A recombinant inbred lines (RILs) population derived from a cross between Zhenshan97B and Miyang46 was used for detecting QTLs with additive effects and additive-by-additive epistasis for rice mesocotyl length. A linkage map consisting of 207 DNA markers,distributing on the 12 chromosomes of rice,was employed for QTL mapping by using software QTL Mapper 1.6 of mixed linear model. Rice mesocotyl length under germination conditions with 4 different FeSO4 concentrations (0, 1.79, 7.16, 14.32 mmol/L) was measured 7 days after planting. A total of 6 QTLs with significant additive effects on chromosome 1, 5 and 9, with variance explained of 3.5%-11.4%, eleven QTLs with significant additive x additive epistatic effects on chromosome 1, 2, 3, 4, 5, 8 were detected, with variance explained of 4.5%-8.1%. In addition, one QTL for environmental interaction (Fe2+ -concentrations) was detected.</p>","PeriodicalId":23770,"journal":{"name":"Yi chuan xue bao = Acta genetica Sinica","volume":"32 7","pages":"712-8"},"PeriodicalIF":0.0,"publicationDate":"2005-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25229000","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Identification of genes related to resistance to Magnaporthe grisea using differential display technique in rice].","authors":"Hai-Ying Zhang,&nbsp;Yong Liu,&nbsp;Dong-Cheng Liu,&nbsp;Xiu-Zhi Wang,&nbsp;Chao Wang,&nbsp;Ling-Xia Wang,&nbsp;Ai-Min Zhang,&nbsp;Ping Li","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Rice blast caused by Magnaporthe grisea is one of the most serious constraints on high productivity. Understanding the mechanism of the infection of Magnaporthe grisea and the change of gene expression after infection is useful to control blast disease in rice. This work presents the isolation of differentially expressed cDNA fragments from rice leaf induced by the inoculum suspension of Magnaporthe grisea using mRNA differential display technique. Total 87 differential expressed cDNA fragments were recoveried and reamplified. The dot-blotting results showed that 6 fragments of 81 were confirmed to be the expression induced by Magnaporthe grisea inoculum. Those fragments were then cloned into vectors for sequencing. Sequence analysis through Internet Blast searching showed that 3 fragments were novel gene fragments. One was homologous with a putative malate synthase gene on rice chromosome 4 with 78% identities of amino acid; one was highly homologous (75% identity) with rice RPR1 gene on chromosome 11, which has a conservative structure of NBS-LRR domain and may be related to signal transduction of rice defense reaction;another one was homologous with a putative thioredoxin gene on rice chromosome 6 with the identity of 72%.</p>","PeriodicalId":23770,"journal":{"name":"Yi chuan xue bao = Acta genetica Sinica","volume":"32 7","pages":"719-25"},"PeriodicalIF":0.0,"publicationDate":"2005-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25229001","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Studies on the cytological characters of the interspecific hybrid F1 among the cultivated species in Gossypium and their genetic relationship].","authors":"Yan-Hui Gao,&nbsp;Shui-Jin Zhu,&nbsp;Dao-Fan Ji","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Interspescific hybridization among four cultivated species in Gossypium (G. herbaceum, G. arboreum, G. hirsutum and G. barbadense) were carried out to produce dispecific hybrids F1, (G. arboreum x G. herbaceum) F1 and (G. hirsutum x G. barbadense) F1, and quadrispecific hybrid F1, which was produced by crossed the chromosome doubled (G. arboreum x G. herbaceum) F1 with (G. hirsutum x G. barbadense) F1. In order to study the evolution relationship among the four cultivated species in Gossypium, the characteristic of chromosome behavior during the meiosis and pollen viability in those interspecific hybrids F1 were studied in this paper. The results showed that the diploid interspecific hybrid, (G. arboreum x G. herbaceum) F1, had a four-chromosome-ring, the chromosome configuration was 2n = 26 = 11 II + 1 IV. And the normal pollen percent was 50.71%, which showed the character of typical gamete semi-sterility, and approved that there was a chromosome translocation between the two diploid cotton species, G. arboreum and G. herbaceum. For the allotetraploid species interspecific hybrid F1, (G. hirsutum x G. barbadense) F1, most of the chromosomes at metaphase I could be paired into bivalents, with a few number of univalents, trivalents, and quardrivalents. The chromosome configuration was 2n = 52 = 0.78 I +22.24 II +0.94 III +0.98 IV, with a normal pollen rate of 54. 84%. The experiment showed that there were a few chromosome translocation or chromosome inversion between the two allotetraploid cotton species, G. hirsutum and G. barbadenses. The meiosis of the quardrispecific hybrid F1 was abnormal, and the loss of chromosomes was common. Most of the chromosomes could not synapse at metaphase I, which led to many univalents and some multivalents. The chromosome configuration of the quardrispecific hybrid F1 was 2n = 52 = 5.45 I +14.41 II +2.44 III +1.59 IV +0.63 V +0.15 VI, and the normal pollen rate was 6.87%, which showed that the relationship of four cultivated cotton species was relatively closed. It is possible to produce a new germplasm with the good characters of the four cultivated species through genetic recombination.</p>","PeriodicalId":23770,"journal":{"name":"Yi chuan xue bao = Acta genetica Sinica","volume":"32 7","pages":"744-52"},"PeriodicalIF":0.0,"publicationDate":"2005-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25229005","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Molecular identification of cytoplasmic male sterility associated gene orf 220 in leaf mustard (Brassica juncea var. multiceps Tsen et Lee)].","authors":"Jing-Hua Yang,&nbsp;Ming-Fang Zhang,&nbsp;Jing-Quan Yu,&nbsp;Tao Wang,&nbsp;Zhu-Jun Chen","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The cytoplasmic male-sterility (CMS) was transferred into leaf mustard by inter-varietal hybridization and subsequent backcrosses cms donor as using tuber mustard. Using specially designed primers, one specific band at 600-700 bp was distinctively amplified by PCR from cms plants of different backcrossed generations. It was genetically stable and cytoplasmically inheritable in the various backcrossed progenies. The sequencing result shows it is a 663 bp fragment with its own initiation and termination codons, presumably encoding for 220 amino acids (named orf 220). This deduced polypeptide had two trans-membrane hydrophilic domains and its N-terminus shared high similarity in amino acids constituents with COX III protein from Oenothera berteriana, ATP8 protein from radish and ORFB protein from sunflower. In addition, RT-PCR analysis showed, orf 220 gene was constitutively expressed in leaves, roots and floral buds. However, the direct evidence of its involvement in expression of cms trait needs further confirmation.</p>","PeriodicalId":23770,"journal":{"name":"Yi chuan xue bao = Acta genetica Sinica","volume":"32 6","pages":"594-9"},"PeriodicalIF":0.0,"publicationDate":"2005-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25189585","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}