[转基因烟草植物中两种植物凝集素基因的表达]。

Zhao-Hua Liu, Zhen-Shan Zhang, Hong-Nian Guo, Yan-Tao Jia, Guang-Yu Zheng, Ying-Chuan Tian
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引用次数: 0

摘要

构建了一种植物表达载体 pBACG,其中含有编码茎苋凝集素(ACA)和改良的茎苋凝集素(GNA)基因的 DNA 序列。用携带上述表达载体的 A. tumefaciens LBA4404 转化烟草烟叶品种 SRI 的叶片外植体。PCR 和 Southern 印迹分析结果表明,ACA 和 GNA 基因都插入了转化烟草植株的基因组中。对从转基因植株中分离出的可溶性蛋白进行的 Western 印迹分析表明,转基因植株合成了 ACA 和 GNA。桃蚜(Myzus persicae)昆虫生物测定结果表明,pBACG 转基因植株对桃蚜具有很强的抗性。转基因植株(T0)及其自交后代(T1)的平均蚜虫抑制率分别达到 83.9% 和 85.3%,表明这两个基因的功能是可遗传的。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
[Expression of two plant agglutinin genes in transgenic tobacco plants].

A plant expression vector pBACG containing the DNA sequence coding for Amaranthus caudatus agglutinin (ACA) and a modified Glanthus nivalis agglutinin (GNA) gene was constructed. Leaf explants of Nicotiana tobacum cv. SRI were transformed with A. tumefaciens LBA4404 harbouring the above expression vector. Results from PCR and Southern blotting analysis showed that both the ACA and GNA gene were inserted into the genome of transformed tobacco plants. Western blottingting analysis of soluble protein isolated from transgenic plants showed that ACA and GNA were synthesized. The results from insect bioassay with peach aphids ( Myzus persicae) revealed that the transgenic plants of pBACG had acquired high resistance against peach aphids. The average aphid-inhibition rate reached up to 83.9% and 85.3% for transgenic plants (T0) and their selfed progenies (T1) respectively,indicating that the functions of these two genes were inheritable.

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