水稻14-3-3基因家族的生物信息学分析

Gu-Lei Jin, Xu-Sheng Wang, Jun Zhu
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引用次数: 0

摘要

利用两步隐马尔可夫模型(HMM)扫描策略,对水稻(Oryza sativa L. ssp)进行检索,鉴定出8个14-3-3样蛋白。粳稻蛋白数据库。其中有4个基因是本研究新发现的。我们通过EST搜索限定了在日本的表达基因。表达分析还显示,每个基因在任何个体内的表达都是不同的,这往往暗示了特定基因的特定功能。氨基酸序列比对表明,特定残基可能具有同工异构体功能。基因结构和染色体定位分析表明,水稻基因组中含有14-3-3蛋白的epsilon和no-epsilon两种形式。此外,我们还分析了水稻14-3-3蛋白家族的进化过程。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
[Bioinformatic analysis of the 14-3-3 gene family in rice].

Using two-step HMM (hidden markov model) scan strategy,eight 14-3-3-like proteins were identified by searching the Oryza sativa L. ssp. japonica protein database. From them four genes were newly detected in this study. We confined the genes expressing in Nipponbare by EST search. Expression analysis also showed each gene expressed diversely within any individual,this tends suggested specific function of particular gene. Alignment of amino acid sequences suggested that there could be isoform function of the specific residues. The analyses of gene structure and chromosome location indicated that rice genome contains both epsilon and no-epsilon forms of 14-3-3 proteins. In addition,we analyzed the evolution of the rice 14-3-3 protein family.

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