小麦抗条锈病新基因YrHua的遗传分析及分子标记[j]。

Zhang-Jun Cao, Xian-Ping Wang, Mei-Nan Wang, Shuang-He Cao, Jin-Xue Jing, Hong-Sheng Shang, Zhen-Qi Li, Xiang-Qi Zhang
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引用次数: 0

摘要

H9020-17-5是一种常见的小麦-华山灰蚜易位系,具有优良的抗小麦条锈病能力。对H9020-17-5 × Mingxian169的F2和BC1居群的遗传分析表明,H9020-17-5对条锈病的抗性是由花山梨单基因控制的显性性状。本研究首次报道了该抗性基因,并将其命名为“花花”。采用AFLP方法对接种条锈病分离物CY30的H9020-17-5 ×明鲜169 F2群体119个个体进行了分析,以确定抗性基因YrHua。结果发现,PM14(301)和PM42(249)两个标记与抗性基因YrHua有连锁,标记与靶基因的遗传距离分别为5.4 cM和2.7 cM。为了便于小麦育种中的标记辅助选择,根据PM14(301)的DNA序列和限制性位点多态性,利用一对特殊引物将两个AFLP标记中的一个转化为PCR标记。我们的研究结果为标记辅助选择提供了有用的工具,并为血花基因的精细定位和基于图谱的克隆奠定了基础。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
[Genetic analysis and molecular markers of a novel stripe rust resistance gene YrHua in wheat originated from Psathyrostachys huashanica Keng].

The H9020-17-5,a common wheat-Psathyrostachys huashanica Keng translocation line, possesses excellent resistance to wheat stripe rust. Genetic analysis of F2 and BC1 populations derived from H9020-17-5 x Mingxian169 indicated that resistance to stripe rust in H9020-17-5 was a dominant character controlling by single gene originated from Ps. huashanica. This resistance gene originated from Ps. huashanica was first reported in the present study and named as YrHua. In order to map the resistance gene YrHua, AFLP approach was employed to analyze the 119 individuals of H9020-17-5 x Mingxian169 F2 population which were inoculated by stripe rust isolate CY30. As a result,two markers, PM14(301) and PM42(249) were found to be linked to the resistance gene YrHua,and the genetic distances between the markers and target gene were 5.4 cM and 2.7 cM, respectively. For the convenience of marker-assisted selection in wheat breeding, one of the two AFLP markers was converted to PCR marker using a pair of special primers based on the DNA sequence of PM14 (301) and the polymorphism of restriction site. Our research results provided a useful tool for marker-assisted selection and laid a foundation of fine mapping and map based cloning of YrHua gene.

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