[Characterization of the beta-actin gene of the rice field eel and its phylogeny in fish].

Lai-Xin Xia, Han-Hua Cheng, Yi-Qing Guo, Rong-Jia Zhou
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Abstract

Beta-actin is a member of the actin family of genes,which play important roles in maintaining cytoskeletal structure, cell motility, cell division, intracellular movements and contractile processes. We report here the identification of a beta-actin cDNA of the rice field eel,a teleost fish with a characteristic of natural sex reversal. The cDNA sequence of this gene was 1860 bp in length,encoding a 375 amino acid protein. Amino acid identities of the beta-actin between the rice field eel and other vertebrates including human, chicken, and other fishes, were more than 98%. RT-PCR showed expression of the rice field eel beta-actin in testis, ovotestis, ovary, heart, liver, spleen and brain,suggesting a ubiquitous expression pattern. Phylogenetic tree including all beta-actin cds of teleost fishes was constructed,which suggested consistently that teleost beta-actin can be classified into four types,but no fish has been found contains all the four types of beta-actin gene in its genome. The results that suggest lineage-specific beta-actin loss might happen in the radical evolution of teleost fishes.

稻田鳗β -肌动蛋白基因的鉴定及其在鱼类中的系统发育。
β -肌动蛋白是肌动蛋白家族的一员,在维持细胞骨架结构、细胞运动、细胞分裂、细胞内运动和收缩过程中起重要作用。我们在这里报道了稻田鳗鱼的β -肌动蛋白cDNA的鉴定,鳝是一种具有自然性别逆转特征的硬骨鱼。该基因全长1860 bp,编码一个375个氨基酸的蛋白。稻田鳗与其他脊椎动物(包括人、鸡和其他鱼类)之间的-肌动蛋白氨基酸一致性超过98%。RT-PCR结果显示,稻田鳗β -肌动蛋白在睾丸、卵睾丸、卵巢、心脏、肝脏、脾脏和大脑中均有表达,表达模式普遍存在。构建了包含硬骨鱼所有β -肌动蛋白cd的系统发育树,结果一致表明硬骨鱼β -肌动蛋白可分为四种类型,但尚未发现在其基因组中包含所有四种类型的β -肌动蛋白基因。结果表明,谱系特异性的-肌动蛋白丢失可能发生在硬骨鱼的激进进化中。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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