[Inheritance analysis and molecular marker selection of genes for wheat spindle streak mosaic disease resistance].

Qing-Ping Zhang, Xiu-E Wang, Yao-Nan Wang, Yan Zhao, Hai-Yan Wang, Su-Ling Wang, Pei-Du Chen
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Abstract

Three wheat spindle streak mosaic viruses (WSSMV) resistant cultivars ('Yining Xiaomai', 'Xu87-633', and 'Xifeng') and one susceptible cultivar ('Zhen9523') were used as parents of 3 crosses in this experiment. WSSMV resistance of the parents, F1, and F2 was evaluated under field condition. Based on the segregation ratios of resistant and susceptible plants in F, and F2 populations, it was deduced that the resistance to WSSMV was dominant and the inheritable factors controlling WSSMV resistance were encoded by the nuclear genome. WSSMV resistances in 'Yining Xiaomai' and 'Xifeng' were controlled by two pairs of alleles, which showed complementary effects. However the resistance in 'Xu-87633' was controlled by a single dominant gene. 266 pairs of SSR primers located on 21 wheat chromosomes were used for polymorphic analysis of the two resistant and the susceptible parents 'Yining Xiaomai' and 'Zhen9523', and 108 of them amplified polymorphic DNA products. By Bulk Segregant Analysis of resistant and susceptible pools, one pair of primer located on chromosome arm 2DS, Xgwm261, were found being linked to WSSMV resistance. The 224 F2 individuals were then amplified with marker Xgwm261. The statistic genetic distance between Xgwm261 and the resistance locus was calculated to be 22.9 cM using the software Mapmaker 3.0.

小麦轴条花叶病抗性基因的遗传分析和分子标记选择。
以3个小麦纺锤条花叶病毒(WSSMV)抗性品种‘伊宁小买’、‘旭87-633’和‘西风’为亲本,1个小麦纺锤条花叶病毒易感品种‘真9523’为亲本。在田间条件下对亲本、F1和F2的WSSMV抗性进行了评价。根据F、F2群体中抗性和感感植株的分离率,推测对WSSMV的抗性为显性,控制WSSMV抗性的遗传因子是由核基因组编码的。‘伊宁小买’和‘西风’的WSSMV抗性由两对等位基因控制,呈互补效应。而“徐-87633”的抗性是由一个显性基因控制的。利用位于21条小麦染色体上的266对SSR引物对易感亲本“益宁小买”和“真9523”进行多态性分析,其中108对引物扩增出多态性DNA产物。通过对抗性和敏感群体的整体分离分析,发现位于2DS染色体臂上的一对引物Xgwm261与WSSMV抗性有关。用标记Xgwm261扩增224f2个体。利用Mapmaker 3.0软件计算出Xgwm261与抗性位点的统计遗传距离为22.9 cM。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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