Virulence最新文献_第7页

Throat microbiota drives alterations in pulmonary alveolar microbiota in patients with septic ARDS. 咽喉微生物群驱动脓毒症 ARDS 患者肺泡微生物群的改变。

IF 5.2 1区农林科学

Virulence Pub Date : 2024-12-01 Epub Date: 2024-05-12 DOI: 10.1080/21505594.2024.2350775

Na-Na Li, Kai Kang, Yang Zhou, Yan-Qi Liu, Qian-Qian Zhang, Pei-Yao Luo, Lei Wang, Ming-Yin Man, Jia-Feng Lv, Xi-Bo Wang, Ya-Hui Peng, Fei-Yu Luan, Yue Li, Jian-Nan Zhang, Yang Chong, Yi-Qi Wang, Chang-Song Wang, Ming-Yan Zhao, Kai-Jiang Yu

{"title":"Throat microbiota drives alterations in pulmonary alveolar microbiota in patients with septic ARDS.","authors":"Na-Na Li, Kai Kang, Yang Zhou, Yan-Qi Liu, Qian-Qian Zhang, Pei-Yao Luo, Lei Wang, Ming-Yin Man, Jia-Feng Lv, Xi-Bo Wang, Ya-Hui Peng, Fei-Yu Luan, Yue Li, Jian-Nan Zhang, Yang Chong, Yi-Qi Wang, Chang-Song Wang, Ming-Yan Zhao, Kai-Jiang Yu","doi":"10.1080/21505594.2024.2350775","DOIUrl":"10.1080/21505594.2024.2350775","url":null,"abstract":"Objectives: The translocation of intestinal flora has been linked to the colonization of diverse and heavy lower respiratory flora in patients with septic ARDS, and is considered a critical prognostic factor for patients.Methods: On the first and third days of ICU admission, BALF, throat swab, and anal swab were collected, resulting in a total of 288 samples. These samples were analyzed using 16S rRNA analysis and the traceability analysis of new generation technology.Results: On the first day, among the top five microbiota species in abundance, four species were found to be identical in BALF and throat samples. Similarly, on the third day, three microbiota species were found to be identical in abundance in both BALF and throat samples. On the first day, 85.16% of microorganisms originated from the throat, 5.79% from the intestines, and 9.05% were unknown. On the third day, 83.52% of microorganisms came from the throat, 4.67% from the intestines, and 11.81% were unknown. Additionally, when regrouping the 46 patients, the results revealed a significant predominance of throat microorganisms in BALF on both the first and third day. Furthermore, as the disease progressed, the proportion of intestinal flora in BALF increased in patients with enterogenic ARDS.Conclusions: In patients with septic ARDS, the main source of lung microbiota is primarily from the throat. Furthermore, the dynamic trend of the microbiota on the first and third day is essentially consistent.It is important to note that the origin of the intestinal flora does not exclude the possibility of its origin from the throat.","PeriodicalId":23747,"journal":{"name":"Virulence","volume":"15 1","pages":"2350775"},"PeriodicalIF":5.2,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11093027/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140912813","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Essential roles of Rad6 in conidial property, stress tolerance, and pathogenicity of Beauveria bassiana. Rad6 在 Beauveria bassiana 的分生孢子特性、应激耐受性和致病性中的重要作用。

IF 5.5 1区农林科学

Virulence Pub Date : 2024-12-01 Epub Date: 2024-06-11 DOI: 10.1080/21505594.2024.2362748

Yi Guan, Haomin He, Yuhan Guo, Longbin Zhang

{"title":"Essential roles of Rad6 in conidial property, stress tolerance, and pathogenicity of Beauveria bassiana.","authors":"Yi Guan, Haomin He, Yuhan Guo, Longbin Zhang","doi":"10.1080/21505594.2024.2362748","DOIUrl":"10.1080/21505594.2024.2362748","url":null,"abstract":"Rad6 functions as a ubiquitin-conjugating protein that regulates cellular processes in many fungal species. However, its role in filamentous entomopathogenic fungi remains poorly understood. This study characterizes Rad6 in Beauveria bassiana, a filamentous fungus widely employed as a critical fungicide globally. The results demonstrate a significant association between Rad6 and conidial properties, heat shock response, and UV-B tolerance. Concurrently, the mutant strain exhibited heightened sensitivity to oxidative stress, cell wall interfering agents, DNA damage stress, and prolonged heat shock. Furthermore, the absence of Rad6 significantly extended the median lethal time (LT50) of Galleria mellonella infected by B. bassiana. This delay could be attributed to reduced Pr1 proteases and extracellular cuticle-degrading enzymes, diminished dimorphic transition rates, and dysregulated antioxidant enzymes. Additionally, the absence of Rad6 had a more pronounced effect on genetic information processing, metabolism, and cellular processes under normal conditions. However, its impact was limited to metabolism in oxidative stress. This study offers a comprehensive understanding of the pivotal roles of Rad6 in conidial and hyphal stress tolerance, environmental adaptation, and the pathogenesis of Beauveria bassiana.","PeriodicalId":23747,"journal":{"name":"Virulence","volume":"15 1","pages":"2362748"},"PeriodicalIF":5.5,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11174126/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141301746","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Research progress on the nonstructural protein 1 (NS1) of influenza a virus. 甲型流感病毒非结构蛋白 1（NS1）的研究进展。

IF 5.5 1区农林科学

Virulence Pub Date : 2024-12-01 Epub Date: 2024-06-25 DOI: 10.1080/21505594.2024.2359470

Xiaoyan Zhang, Yuying Zhang, Fanhua Wei

引用次数: 0

Prevalence and transmission of pretreatment drug resistance in people living with HIV-1 in Shanghai China, 2017-2021. 2017-2021年中国上海HIV-1感染者预处理耐药性的流行和传播情况。

IF 5.5 1区农林科学

Virulence Pub Date : 2024-12-01 Epub Date: 2024-06-27 DOI: 10.1080/21505594.2024.2373105

Min Zhang, Yingying Ma, Zhenyan Wang, Gang Wang, Qianying Wang, Xin Li, Feng Lin, Chiyu Zhang

{"title":"Prevalence and transmission of pretreatment drug resistance in people living with HIV-1 in Shanghai China, 2017-2021.","authors":"Min Zhang, Yingying Ma, Zhenyan Wang, Gang Wang, Qianying Wang, Xin Li, Feng Lin, Chiyu Zhang","doi":"10.1080/21505594.2024.2373105","DOIUrl":"10.1080/21505594.2024.2373105","url":null,"abstract":"The implementation of pretreatment drug-resistance (PDR) surveillance among people living with HIV-1 (PLWH) is a top priority in countries using efavirenz (EFV)/nevirapine (NVP) for first-line ART. In this study, we assessed the prevalence of PDR among PLWH in Shanghai, China during 2017-2021, and to reveal PDR transmission between Shanghai and other regions of China. A total of 5050 PLWH not on ART during 2017-2021 were included. Partial HIV-1 pol sequences were amplified, sequenced, and analysed for drug-resistance mutations (DRMs). Besides, transmission network of PDR variants was inferred using HIV-TRACE. The overall prevalence of PDR was 4.8% (242/5050; 95% CI, 4.2-5.4). Prevalence of NNRTI-associated PDR was 3.9% (95% CI, 3.4-4.5), higher than those of NRTI-associated (0.8%; 95% CI, 0.5-1.1) and PI-associated PDR (0.9%; 95% CI, 0.6-1.2). High prevalence of PDR (especially high-level resistance) to EFV (132/5050, 2.6%) and NVP (137/5050, 2.7%) were found. CRF01_AE (46.0%) was the predominant HIV-1 genotype with any DRMs, followed by CRF55_01B (21.0%), and CRF07_BC (15.1%). Two NRTI-associated (S68G/N/R and T215A/N/S/Y), five NNRTI-associated (V179D/E/T/L, K103N/R/S/T, E138A/G/K, V106M/I/A and Y181C/I) and two PI-associated mutations (M46I/L/V and Q58E) were the most common observed DRMs in PDR patients in Shanghai. The vast majority of S68G occurred in CRF01_AE (45%). M46I/L/V and Q58E showed a relatively high prevalence in CRF01_AE (4.1%) and CRF07_BC (12.6%). Transmission network analyses demonstrated cross-regional transmission links of PDR variants between Shanghai and other regions of China, which was mainly driven by the potential low-level DRM V179D/E. These results provide crucial information for clinical decision making of first-line ART in PLWH with PDR.","PeriodicalId":23747,"journal":{"name":"Virulence","volume":"15 1","pages":"2373105"},"PeriodicalIF":5.5,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11212556/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141459624","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evaluation of the immune responses of biological adjuvant bivalent vaccine with three different insertion modes for ND and IBD. 评估具有三种不同插入模式的 ND 和 IBD 生物佐剂二价疫苗的免疫反应。

IF 5.5 1区农林科学

Virulence Pub Date : 2024-12-01 Epub Date: 2024-08-05 DOI: 10.1080/21505594.2024.2387181

Wenying Sun, Shuang Li, Dun Niu, Ruihan Qin, Huimin Li, Zhiqiang Xue, Yunpeng Guo, Jinmiao Liu, Yijia Liu, Xinghao Jiang, Jiechao Yin, Xiaochen Guo, Guiping Ren

{"title":"Evaluation of the immune responses of biological adjuvant bivalent vaccine with three different insertion modes for ND and IBD.","authors":"Wenying Sun, Shuang Li, Dun Niu, Ruihan Qin, Huimin Li, Zhiqiang Xue, Yunpeng Guo, Jinmiao Liu, Yijia Liu, Xinghao Jiang, Jiechao Yin, Xiaochen Guo, Guiping Ren","doi":"10.1080/21505594.2024.2387181","DOIUrl":"10.1080/21505594.2024.2387181","url":null,"abstract":"Infectious bursal disease (IBD) is a widespread problem in the poultry industry, and vaccination is the primary preventive method. However, moderately virulent vaccines may damage the bursa, necessitating the development of a safe and effective vaccine. The Newcastle disease virus (NDV) has been explored as a vector for vaccine development. In this study, reverse genetic technology was used to obtain three recombinant viruses, namely, rClone30-VP2L (P/M)-chGM-CSF (NP), rClone30-chGM-CSF (P/M)-VP2L (NP), and rClone30-VP2L-chGM-CSF (P/M). Animal experiments showed that the three biological adjuvant bivalent vaccines effectively increased anti-NDV and anti-infectious bursal disease virus (IBDV) titres, enhancing both humoral and cellular immune responses in chickens without leading to any harm. Amongst the three biological adjuvant bivalent vaccines, the rClone30-chGM-CSF (P/M)-VP2L (NP) group had higher levels of anti-NDV antibodies at 14 days after the first immunization and stimulated a greater humoral immune response in 7-10 days. While, the rClone30-VP2L (P/M)-chGM-CSF (NP) group was the most effective in producing a higher level of IBDV antibody response. In conclusion, these three vaccines can induce immune responses more rapidly and effectively, streamline production processes, be cost-effective, and provide a new avenue for the development of Newcastle disease (ND) and IBD bivalent vaccines.","PeriodicalId":23747,"journal":{"name":"Virulence","volume":"15 1","pages":"2387181"},"PeriodicalIF":5.5,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11302549/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141890241","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Emergence of Staphylococcus argenteus in pediatrics: Molecular insights from a hospital in East China. 儿科中出现的金黄色葡萄球菌：来自华东一家医院的分子研究。

IF 5.5 1区农林科学

Virulence Pub Date : 2024-12-01 Epub Date: 2024-08-27 DOI: 10.1080/21505594.2024.2396477

Chao Fang, Zheng Zhou, Jianping Li, Mingming Zhou

{"title":"Emergence of Staphylococcus argenteus in pediatrics: Molecular insights from a hospital in East China.","authors":"Chao Fang, Zheng Zhou, Jianping Li, Mingming Zhou","doi":"10.1080/21505594.2024.2396477","DOIUrl":"10.1080/21505594.2024.2396477","url":null,"abstract":"Staphylococcus argenteus is a novel species within the Staphylococcus aureus complex and can cause serious bloodstream infections (BSIs) in humans, which have been mainly reported in adults, especially the elderly. In this study, we analyzed the molecular characterization of a strain of S. argenteus (22WJ8192) isolated from the peripheral vein blood sample of a seven-month-old female infant in Eastern China. The 22WJ8192 belonged to sequence type (ST)2250 and harbored six antibiotic-resistance genes and 53 virulence genes and was resistant to penicillin. Additionally, we conducted a comparative analysis of the molecular characteristics of S. argenteus sourced from various origins within the dataset, predominantly from the National Center for Biotechnology Information Collection (NCBI) genome database. Antibiotic-resistance genes blaR1, blaI_of_Z, blaZ, fosB-Saur, tet(L), aph(3\")-IIIa, mecA, and dfrG were more prevalent among the strains of human origin. Virulence genes lukF-PV, sak, sdrE, scn, sdrC, and sdrD were more prevalent among strains of human origin. The presence of antibiotic-resistance genes blaR1, blaI_of_Z, blaZ, fosB-Saur, and aph(3\")-IIIa in strain 22WJ8192 was also more common among strains of human origin in the dataset. Conversely, the antibiotic-resistance genes tet(L), mecA, and dfrG, typically found in strains of human origin, were not detected in 22WJ8192. Additionally, virulence genes lukF-PV, sak, sdrE, scn, sdrC, and sdrD present in 22WJ8192 exhibited a higher prevalence among strains of human origin in the dataset. In conclusion, this study emphasizes the potential of S. argenteus ST2250 to induce severe bloodstream infections in infants, shedding light on the molecular characteristics of this strain.","PeriodicalId":23747,"journal":{"name":"Virulence","volume":" ","pages":"2396477"},"PeriodicalIF":5.5,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11364062/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142056657","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

TolC facilitates the intracellular survival and immunomodulation of Salmonella Typhi in human host cells. TolC 有助于伤寒沙门氏菌在人类宿主细胞中的胞内存活和免疫调节。

IF 5.5 1区农林科学

Virulence Pub Date : 2024-12-01 Epub Date: 2024-09-09 DOI: 10.1080/21505594.2024.2395831

Ashraf Hussain, Eugene Boon Beng Ong, Prabha Balaram, Asma Ismail, Phua Kia Kien

{"title":"TolC facilitates the intracellular survival and immunomodulation of Salmonella Typhi in human host cells.","authors":"Ashraf Hussain, Eugene Boon Beng Ong, Prabha Balaram, Asma Ismail, Phua Kia Kien","doi":"10.1080/21505594.2024.2395831","DOIUrl":"10.1080/21505594.2024.2395831","url":null,"abstract":"Salmonella enterica serovar Typhi (S. Typhi) causes typhoid fever, a systemic infection that affects millions of people worldwide. S. Typhi can invade and survive within host cells, such as intestinal epithelial cells and macrophages, by modulating their immune responses. However, the immunomodulatory capability of S. Typhi in relation to TolC-facilitated efflux pump function remains unclear. The role of TolC, an outer membrane protein that facilitates efflux pump function, in the invasion and immunomodulation of S. Typhi, was studied in human intestinal epithelial cells and macrophages. The tolC deletion mutant of S. Typhi was compared with the wild-type and its complemented strain in terms of their ability to invade epithelial cells, survive and induce cytotoxicity in macrophages, and elicit proinflammatory cytokine production in macrophages. The tolC mutant, which has a defective outer membrane, was impaired in invading epithelial cells compared to the wild-type strain, but the intracellular presence of the tolC mutant exhibited greater cytotoxicity and induced higher levels of proinflammatory cytokines (IL-1β and IL-8) in macrophages compared to the wild-type strain. These effects were reversed by complementing the tolC mutant with a functional tolC gene. Our results suggest that TolC plays a role in S. Typhi to efficiently invade epithelial cells and suppress host immune responses during infection. TolC may be a potential target for the development of novel therapeutics against typhoid fever.","PeriodicalId":23747,"journal":{"name":"Virulence","volume":" ","pages":"2395831"},"PeriodicalIF":5.5,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11385165/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142056658","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Akkermansia muciniphila: A promising probiotic against inflammation and metabolic disorders. Akkermansia muciniphila：一种很有前景的抗炎症和代谢紊乱的益生菌。

IF 5.5 1区农林科学

Virulence Pub Date : 2024-12-01 Epub Date: 2024-08-27 DOI: 10.1080/21505594.2024.2375555

Yanqing Zhao, Huijun Yang, Peng Wu, Shuguo Yang, Wenkun Xue, Biao Xu, Sirui Zhang, Bin Tang, Daoxiu Xu

{"title":"Akkermansia muciniphila: A promising probiotic against inflammation and metabolic disorders.","authors":"Yanqing Zhao, Huijun Yang, Peng Wu, Shuguo Yang, Wenkun Xue, Biao Xu, Sirui Zhang, Bin Tang, Daoxiu Xu","doi":"10.1080/21505594.2024.2375555","DOIUrl":"10.1080/21505594.2024.2375555","url":null,"abstract":"Metabolic disease is a worldwide epidemic that has become a public health problem. Gut microbiota is considered to be one of the important factors that maintain human health by regulating host metabolism. As an abundant bacterium in the host gut, A. muciniphila regulates metabolic and immune functions, and protects gut health. Multiple studies have indicated that alterations in the abundance of A. muciniphila are associated with various diseases, including intestinal inflammatory diseases, obesity, type 2 diabetes mellitus, and even parasitic diseases. Beneficial effects were observed not only in live A. muciniphila, but also in pasteurized A. muciniphila, A. muciniphila-derived extracellular vesicles, outer membrane, and secreted proteins. Although numerous studies have only proven the simple correlation between multiple diseases and A. muciniphila, an increasing number of studies in animal models and preclinical models have demonstrated that the beneficial impacts shifted from correlations to in-depth mechanisms. In this review, we provide a comprehensive view of the beneficial effects of A. muciniphila on different diseases and summarize the potential mechanisms of action of A. muciniphila in the treatment of diseases. We provide a comprehensive understanding of A. muciniphila for improving host health and discuss the perspectives of A. muciniphila in the future studies.","PeriodicalId":23747,"journal":{"name":"Virulence","volume":"15 1","pages":"2375555"},"PeriodicalIF":5.5,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11364076/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142081761","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Pathogenicity and virulence of Yersinia. 耶尔森氏菌的致病性和毒性。

IF 5.2 1区农林科学

Virulence Pub Date : 2024-12-01 Epub Date: 2024-02-22 DOI: 10.1080/21505594.2024.2316439

Jarett A Seabaugh, Deborah M Anderson

{"title":"Pathogenicity and virulence of Yersinia.","authors":"Jarett A Seabaugh, Deborah M Anderson","doi":"10.1080/21505594.2024.2316439","DOIUrl":"10.1080/21505594.2024.2316439","url":null,"abstract":"The genus Yersinia includes human, animal, insect, and plant pathogens as well as many symbionts and harmless bacteria. Within this genus are Yersinia enterocolitica and the Yersinia pseudotuberculosis complex, with four human pathogenic species that are highly related at the genomic level including the causative agent of plague, Yersinia pestis. Extensive laboratory, field work, and clinical research have been conducted to understand the underlying pathogenesis and zoonotic transmission of these pathogens. There are presently more than 500 whole genome sequences from which an evolutionary footprint can be developed that details shared and unique virulence properties. Whereas the virulence of Y. pestis now seems in apparent homoeostasis within its flea transmission cycle, substantial evolutionary changes that affect transmission and disease severity continue to ndergo apparent selective pressure within the other Yersiniae that cause intestinal diseases. In this review, we will summarize the present understanding of the virulence and pathogenesis of Yersinia, highlighting shared mechanisms of virulence and the differences that determine the infection niche and disease severity.","PeriodicalId":23747,"journal":{"name":"Virulence","volume":"15 1","pages":"2316439"},"PeriodicalIF":5.2,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10896167/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139933145","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Escherichia coli and HPV16 coinfection may contribute to the development of cervical cancer. 大肠杆菌和 HPV16 共同感染可能会导致宫颈癌的发生。

IF 5.2 1区农林科学

Virulence Pub Date : 2024-12-01 Epub Date: 2024-02-21 DOI: 10.1080/21505594.2024.2319962

Qin Zou, Yingying Wu, ShuaiShuai Zhang, Shu Li, Siyue Li, Yan Su, Lei Zhang, Qian Li, Hua Zou, Xinyuan Zhang, Teng Wang, Shuang Liang, Jun Yang, Chunli Li

{"title":"Escherichia coli and HPV16 coinfection may contribute to the development of cervical cancer.","authors":"Qin Zou, Yingying Wu, ShuaiShuai Zhang, Shu Li, Siyue Li, Yan Su, Lei Zhang, Qian Li, Hua Zou, Xinyuan Zhang, Teng Wang, Shuang Liang, Jun Yang, Chunli Li","doi":"10.1080/21505594.2024.2319962","DOIUrl":"10.1080/21505594.2024.2319962","url":null,"abstract":"Persistent human papillomavirus HPV infection is a necessary but insufficient condition for cervical cancer. Microorganisms are crucial environmental factors in cancers susceptibility and progression, recently attracting considerable attention. This study aimed to determine the infection status and relationship between high-risk HPV (HR-HPV) and lower genital tract infectious pathogens in cervical cancer and its precursors. From a retrospective and a prospective cohort analysis, Escherichia coli (E. coli) dominated the pathogens isolated from cervical discharges, and an isolation rate uptrend has been shown recently. HPV16 and E. coli's coinfection rate gradually increased with the severity of cervical intraepithelial neoplasia. The adhesion and invasion abilities of the isolated E. coli to HPV16-positive SiHa cells were evaluated in vitro. The TCGA database and cervical tissues samples analysis showed that IL-10 was upregulated in cervical cancer. IL-10 expression levels increased in tissue samples with the severity of cervical cancer and its precursors with HPV16 and E. coli coinfection. Although no significant changes in IL-10 production were observed in the co-culture supernatant, we hypothesized that Treg immune cells in the tumour microenvironment might be responsible for the local IL-10 upregulation, according to our data showing Foxp3 upregulation and an upward trend with the cervical intraepithelial neoplasia grading to cancer and tumours with E. coli and HPV16 coinfection. Our data provide insights into the possible role of E. coli in cervical cancer progression and suggest that the application of HPV and E. coli screening programs may be an effective strategy to relieve the burden of cervical cancer and its precursor lesions.","PeriodicalId":23747,"journal":{"name":"Virulence","volume":"15 1","pages":"2319962"},"PeriodicalIF":5.2,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10883084/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139913584","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0