Virulence最新文献_第7页

LGG promotes activation of intestinal ILC3 through TLR2 receptor and inhibits salmonella typhimurium infection in mice. LGG 通过 TLR2 受体促进肠道 ILC3 的活化，抑制小鼠鼠伤寒沙门氏菌感染。

IF 5.5 1区农林科学

Virulence Pub Date : 2024-12-01 Epub Date: 2024-07-30 DOI: 10.1080/21505594.2024.2384553

Junhong Wang, Ming Gao, Jiarui Wang, Yan Zeng, Chunfeng Wang, Xin Cao

{"title":"LGG promotes activation of intestinal ILC3 through TLR2 receptor and inhibits salmonella typhimurium infection in mice.","authors":"Junhong Wang, Ming Gao, Jiarui Wang, Yan Zeng, Chunfeng Wang, Xin Cao","doi":"10.1080/21505594.2024.2384553","DOIUrl":"10.1080/21505594.2024.2384553","url":null,"abstract":"Salmonella is a foodborne pathogen that causes disruption of intestinal mucosal immunity, leading to acute gastroenteritis in the host. In this study, we found that Salmonella Typhimurium (STM) infection of the intestinal tract of mice led to a significant increase in the proportion of Lacticaseibacillus, while the secretion of IL-22 from type 3 innate lymphoid cells (ILC3) increased significantly. Feeding Lacticaseibacillus rhamnosus GG (LGG) effectively alleviated the infection of STM in the mouse intestines. TLR2-/- mice experiments found that TLR2-expressing dendritic cells (DCs) are crucial for LGG's activation of ILC3. Subsequent in vitro experiments showed that heat-killed LGG (HK-LGG) could promote DCs to secrete IL-23, which in turn further promotes the activation of ILC3 and the secretion of IL-22. Finally, organoid experiments further verified that IL-22 secreted by ILC3 can enhance the intestinal mucosal immune barrier and inhibit STM infection. This study demonstrates that oral administration of LGG is a potential method for inhibiting STM infection.","PeriodicalId":23747,"journal":{"name":"Virulence","volume":null,"pages":null},"PeriodicalIF":5.5,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11296546/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141856635","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Correction. 更正。

IF 5.2 1区农林科学

Virulence Pub Date : 2024-12-01 Epub Date: 2024-06-09 DOI: 10.1080/21505594.2024.2363658

引用次数: 0

M1-type polarized macrophage contributes to brain damage through CXCR3.2/CXCL11 pathways after RGNNV infection in grouper. 石斑鱼感染 RGNNV 后，M1 型极化巨噬细胞通过 CXCR3.2/CXCL11 途径造成脑损伤。

IF 5.2 1区农林科学

Virulence Pub Date : 2024-12-01 Epub Date: 2024-05-23 DOI: 10.1080/21505594.2024.2355971

Kaishan Liang, Minlin Zhang, Jiantao Liang, Xiaoling Zuo, Xianze Jia, Jinhong Shan, Zongyang Li, Jie Yu, Zijie Xuan, Liyuan Luo, Huihong Zhao, Songyong Gan, Ding Liu, Qiwei Qin, Qing Wang

{"title":"M1-type polarized macrophage contributes to brain damage through CXCR3.2/CXCL11 pathways after RGNNV infection in grouper.","authors":"Kaishan Liang, Minlin Zhang, Jiantao Liang, Xiaoling Zuo, Xianze Jia, Jinhong Shan, Zongyang Li, Jie Yu, Zijie Xuan, Liyuan Luo, Huihong Zhao, Songyong Gan, Ding Liu, Qiwei Qin, Qing Wang","doi":"10.1080/21505594.2024.2355971","DOIUrl":"10.1080/21505594.2024.2355971","url":null,"abstract":"The vertebrate central nervous system (CNS) is the most complex system of the body. The CNS, especially the brain, is generally regarded as immune-privileged. However, the specialized immune strategies in the brain and how immune cells, specifically macrophages in the brain, respond to virus invasion remain poorly understood. Therefore, this study aimed to examine the potential immune response of macrophages in the brain of orange-spotted groupers (Epinephelus coioides) following red-spotted grouper nervous necrosis virus (RGNNV) infection. We observed that RGNNV induced macrophages to produce an inflammatory response in the brain of orange-spotted grouper, and the macrophages exhibited M1-type polarization after RGNNV infection. In addition, we found RGNNV-induced macrophage M1 polarization via the CXCR3.2- CXCL11 pathway. Furthermore, we observed that RGNNV triggered M1 polarization in macrophages, resulting in substantial proinflammatory cytokine production and subsequent damage to brain tissue. These findings reveal a unique mechanism for brain macrophage polarization, emphasizing their role in contributing to nervous tissue damage following viral infection in the CNS.","PeriodicalId":23747,"journal":{"name":"Virulence","volume":null,"pages":null},"PeriodicalIF":5.2,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11123556/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140923264","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

In turkeys, unlike chickens, the non-structural NS1 protein does not play a significant role in the replication and tissue tropism of the H7N1 avian influenza virus. 与鸡不同，火鸡的非结构性 NS1 蛋白在 H7N1 禽流感病毒的复制和组织滋养过程中并不发挥重要作用。

IF 5.5 1区农林科学

Virulence Pub Date : 2024-12-01 Epub Date: 2024-07-16 DOI: 10.1080/21505594.2024.2379371

Maryna Kuryshko, Maria Landmann, Christine Luttermann, Reiner Ulrich, Elsayed M Abdelwhab

{"title":"In turkeys, unlike chickens, the non-structural NS1 protein does not play a significant role in the replication and tissue tropism of the H7N1 avian influenza virus.","authors":"Maryna Kuryshko, Maria Landmann, Christine Luttermann, Reiner Ulrich, Elsayed M Abdelwhab","doi":"10.1080/21505594.2024.2379371","DOIUrl":"10.1080/21505594.2024.2379371","url":null,"abstract":"The economic losses caused by high pathogenicity (HP) avian influenza viruses (AIV) in the poultry industry worldwide are enormous. Although chickens and turkeys are closely related Galliformes, turkeys are thought to be a bridging host for the adaptation of AIV from wild birds to poultry because of their high susceptibility to AIV infections. HPAIV evolve from low pathogenicity (LP) AIV after circulation in poultry through mutations in different viral proteins, including the non-structural protein (NS1), a major interferon (IFN) antagonist of AIV. At present, it is largely unknown whether the virulence determinants of HPAIV are the same in turkeys and chickens. Previously, we showed that mutations in the NS1 of HPAIV H7N1 significantly reduced viral replication in chickens in vitro and in vivo. Here, we investigated the effect of NS1 on the replication and virulence of HPAIV H7N1 in turkeys after inoculation with recombinant H7N1 carrying a naturally truncated wild-type NS1 (with 224 amino-acid \"aa\" in length) or an extended NS1 with 230-aa similar to the LP H7N1 ancestor. There were no significant differences in multiple-cycle viral replication or in the efficiency of NS1 in blocking IFN induction in the cell culture. Similarly, all viruses were highly virulent in turkeys and replicated at similar levels in various organs and swabs collected from the inoculated turkeys. These results suggest that NS1 does not play a role in the virulence or replication of HPAIV H7N1 in turkeys and further indicate that the genetic determinants of HPAIV differ in these two closely related galliform species.","PeriodicalId":23747,"journal":{"name":"Virulence","volume":null,"pages":null},"PeriodicalIF":5.5,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11259080/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141627821","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Streptococcus suis subtilisin-like serine proteases SspA-1 and SspA-2 interplay with complement C3a and C5a to facilitate bacterial immune evasion and infection. 猪链球菌枯草蛋白样丝氨酸蛋白酶 SspA-1 和 SspA-2 与补体 C3a 和 C5a 相互作用，促进细菌的免疫逃避和感染。

IF 5.2 1区农林科学

Virulence Pub Date : 2024-12-01 Epub Date: 2024-01-16 DOI: 10.1080/21505594.2023.2301246

Simin Deng, Junhui Liao, Haojie Li, Jiali Xu, Jingyan Fan, Jing Xia, Jing Wang, Lei Lei, Mianmian Chen, Yue Han, Ruidong Zhai, Chang Zhou, Rui Zhou, Changyong Cheng, Houhui Song

{"title":"Streptococcus suis subtilisin-like serine proteases SspA-1 and SspA-2 interplay with complement C3a and C5a to facilitate bacterial immune evasion and infection.","authors":"Simin Deng, Junhui Liao, Haojie Li, Jiali Xu, Jingyan Fan, Jing Xia, Jing Wang, Lei Lei, Mianmian Chen, Yue Han, Ruidong Zhai, Chang Zhou, Rui Zhou, Changyong Cheng, Houhui Song","doi":"10.1080/21505594.2023.2301246","DOIUrl":"10.1080/21505594.2023.2301246","url":null,"abstract":"Streptococcus suis (S. suis), a significant zoonotic bacterial pathogen impacting swine and human, is associated with severe systemic diseases such as streptococcal toxic shock-like syndrome, meningitis, septicaemia, and abrupt fatality. The multifaceted roles of complement components C5a and C3a extend to orchestrating inflammatory cells recruitment, oxidative burst induction, and cytokines release. Despite the pivotal role of subtilisin-like serine proteases in S. suis pathogenicity, their involvement in immune evasion remains underexplored. In the present study, we identify two cell wall-anchored subtilisin-like serine proteases in S. suis, SspA-1 and SspA-2, as binding partners for C3a and C5a. Through Co-Immunoprecipitation, Enzyme-Linked Immunosorbent and Far-Western Blotting Assays, we validate their interactions with the aforementioned components. However, SspA-1 and SspA-2 have no cleavage activity against complement C3a and C5a performed by Cleavage assay. Chemotaxis assays reveal that recombinant SspA-1 and SspA-2 effectively attenuate monocyte chemotaxis towards C3a and C5a. Notably, the ΔsspA-1, ΔsspA-1, and ΔsspA-1/2 mutant strains exhibit compromised survival in blood, and resistance of opsonophagocytosis, alongside impaired survival in blood and in vivo colonization compared to the parental strain SC-19. Critical insights from the murine and Galleria mellonella larva infection models further underscore the significance of sspA-1 in altering mortality rates. Collectively, our findings indicate that SspA-1 and SspA-2 are novel binding proteins for C3a and C5a, thereby shedding light on their pivotal roles in S. suis immune evasion and the pathogenesis.","PeriodicalId":23747,"journal":{"name":"Virulence","volume":null,"pages":null},"PeriodicalIF":5.2,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10795781/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139088675","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The multifaceted interactions between Newcastle disease virus proteins and host proteins: a systematic review. 新城疫病毒蛋白质与宿主蛋白质之间多方面的相互作用：系统综述。

IF 5.2 1区农林科学

Virulence Pub Date : 2024-12-01 Epub Date: 2024-01-09 DOI: 10.1080/21505594.2023.2299182

Xiaolong Lu, Xiaoquan Wang, Xiufan Liu, Xiaowen Liu

{"title":"The multifaceted interactions between Newcastle disease virus proteins and host proteins: a systematic review.","authors":"Xiaolong Lu, Xiaoquan Wang, Xiufan Liu, Xiaowen Liu","doi":"10.1080/21505594.2023.2299182","DOIUrl":"10.1080/21505594.2023.2299182","url":null,"abstract":"Newcastle disease virus (NDV) typically induces severe illness in poultry and results in significant economic losses for the worldwide poultry sector. NDV, an RNA virus with a single-stranded negative-sense genome, is susceptible to mutation and immune evasion during viral transmission, thus imposing enormous challenges to avian health and poultry production. NDV is composed of six structural proteins and two nonstructural proteins that exert pivotal roles in viral infection and antiviral responses by interacting with host proteins. Nowadays, there is a particular focus on the mechanisms of virus-host protein interactions in NDV research, yet a comprehensive overview of such research is still lacking. Herein, we briefly summarize the mechanisms regarding the effects of virus-host protein interaction on viral infection, pathogenesis, and host immune responses. This review can not only enhance the present comprehension of the mechanism underlying NDV and host interplay, but also furnish a point of reference for the advancement of antiviral measures.","PeriodicalId":23747,"journal":{"name":"Virulence","volume":null,"pages":null},"PeriodicalIF":5.2,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10793697/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139404600","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Time to positivity of Klebsiella pneumoniae in blood cultures as prognostic marker in patients with intra-abdominal infection: A retrospective study. 血液培养中肺炎克雷伯菌阳性时间作为腹腔内感染患者的预后指标：一项回顾性研究。

IF 5.2 1区农林科学

Virulence Pub Date : 2024-12-01 Epub Date: 2024-03-28 DOI: 10.1080/21505594.2024.2329397

Yong-Ye Yang, I-Ting Tsai, Chung-Hsu Lai, Chih-Ping Chen, Chia-Chi Chen, Yin-Chou Hsu

{"title":"Time to positivity of Klebsiella pneumoniae in blood cultures as prognostic marker in patients with intra-abdominal infection: A retrospective study.","authors":"Yong-Ye Yang, I-Ting Tsai, Chung-Hsu Lai, Chih-Ping Chen, Chia-Chi Chen, Yin-Chou Hsu","doi":"10.1080/21505594.2024.2329397","DOIUrl":"10.1080/21505594.2024.2329397","url":null,"abstract":"Klebsiella pneumoniae is a common causative pathogen of intra-abdominal infection with concomitant bacteraemia, leading to a significant mortality risk. The time to positivity (TTP) of blood culture is postulated to be a prognostic factor in bacteraemia caused by other species. Therefore, this study aimed to investigate the prognostic value of TTP in these patients. The single-centred, retrospective, observational cohort study was conducted between 1 July 2016 and 30 June 2021. All adult emergency department patients with diagnosis of intra-abdominal infection and underwent blood culture collection which yield K. pneumoniae during this period were enrolled. A total of 196 patients were included in the study. The overall 30-day mortality rate was 12.2% (24/196), and the median TTP of the studied cohort was 12.3 h (10.5-15.8 h). TTP revealed a moderate 30-day mortality discriminative ability (area under the curve 0.73, p < 0.001). Compared with the late TTP group (>12 h, N = 109), patients in the early TTP (≤12 h, N = 87) group had a significantly higher risk of 30-day morality (21.8% vs. 4.6%, p < 0.01) and other adverse outcomes. Furthermore, TTP (odds ratio [OR] = 0.79, p = 0.02), Pitt bacteraemia score (OR = 1.30, p = 0.03), and implementation of source control (OR = 0.06, p < 0.01) were identified as independent factors related to 30-day mortality risk in patients with intra-abdominal infection and K. pneumoniae bacteraemia. Therefore, physicians can use TTP for prognosis stratification in these patients.","PeriodicalId":23747,"journal":{"name":"Virulence","volume":null,"pages":null},"PeriodicalIF":5.2,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10984124/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140319396","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Coincidence of antibodies against Hwp1 and ASCA, two distinct molecular targets of Candida albicans, reinforces the link between this fungal species and coeliac disease. 针对 Hwp1 和 ASCA（白色念珠菌的两个不同分子靶标）的抗体同时出现，加强了这种真菌与乳糜泻之间的联系。

IF 5.2 1区农林科学

Virulence Pub Date : 2024-12-01 Epub Date: 2024-03-26 DOI: 10.1080/21505594.2024.2334085

Boualem Sendid, Christopher Cao, Jean-Frederic Colombel, Daniel Poulain

{"title":"Coincidence of antibodies against Hwp1 and ASCA, two distinct molecular targets of Candida albicans, reinforces the link between this fungal species and coeliac disease.","authors":"Boualem Sendid, Christopher Cao, Jean-Frederic Colombel, Daniel Poulain","doi":"10.1080/21505594.2024.2334085","DOIUrl":"10.1080/21505594.2024.2334085","url":null,"abstract":"Candida albicans is an immunogen for anti-Saccharomyces cerevisiae antibodies (ASCA), a serological marker of Crohn's disease. ASCA has also been reported in other autoimmune diseases, including coeliac disease (CeD). A strong antibody response against Hwp1, a protein associated with invasive hyphal form of C. albicans which presents peptide sequence homologies with gliadin, has also been described in CeD. This observation supports the hypothesis that C. albicans hyphal transition in C. albicans may trigger CeD onset through a mechanism of molecular/antigenic mimicry. In this study, we assessed whether the anti-C. albicans oligomannose and anti-Hwp1 protein responses may be linked despite their different pathophysiological significance. The measurement of ASCA levels in a cohort of patients involved in our previous Hwp1 study showed a significant correlation between the two biomarkers. This new observation further reinforces the link between C. albicans and CeD.","PeriodicalId":23747,"journal":{"name":"Virulence","volume":null,"pages":null},"PeriodicalIF":5.2,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10968292/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140289062","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Analyzing the factors affecting virus invasion by quantitative single-particle analysis. 通过单粒子定量分析，分析影响病毒入侵的因素。

IF 5.5 1区农林科学

Virulence Pub Date : 2024-12-01 Epub Date: 2024-06-23 DOI: 10.1080/21505594.2024.2367671

Yi-Ning Hou, Li-Juan Zhang, Lei Du, Dan-Dan Fu, Jing Li, Liu Liu, Peng-Fei Xu, Ya-Wen Zheng, Dai-Wen Pang, Hong-Wu Tang

{"title":"Analyzing the factors affecting virus invasion by quantitative single-particle analysis.","authors":"Yi-Ning Hou, Li-Juan Zhang, Lei Du, Dan-Dan Fu, Jing Li, Liu Liu, Peng-Fei Xu, Ya-Wen Zheng, Dai-Wen Pang, Hong-Wu Tang","doi":"10.1080/21505594.2024.2367671","DOIUrl":"10.1080/21505594.2024.2367671","url":null,"abstract":"Viral diseases are among the main threats to public health. Understanding the factors affecting viral invasion is important for antiviral research. Until now, it was known that most viruses have very low plaque-forming unit (PFU)-to-particle ratios. However, further investigation is required to determine the underlying factors. Here, using quantitative single-particle analysis methods, the invasion of Semliki Forest virus (SFV), Japanese encephalitis virus (JEV), and influenza A virus (IAV) containing attachment to the cell surface, entry into the cell, transport towards the cell interior, and fusion with endosomes to release nucleocapsids were quantitatively analysed in parallel. It was found that for SFV with an PFU-to-particle ratio of approximately 1:2, an entry efficiency of approximately 31% limited infection. For JEV, whose PFU-to-particle ratio was approximately 1:310, an attachment efficiency of approximately 27% and an entry efficiency of 10% were the main factors limiting its infection. Meanwhile, for IAV with PFU-to-particle ratios of 1:8100, 5% attachment efficiency, 9% entry efficiency, and 53% fusion efficiency significantly limited its infection. These results suggest that viruses with different infectivities have different limited steps in the invasion process. Moreover, there are significant differences in attachment efficiencies among viruses, emphasizing the pivotal role of attachment in viral invasion. The influence of the virus purification method on virus invasion was also investigated. This study, for the first time, reports the efficiencies of different stages of virus invasion, leading to a better understanding of virus invasion and providing a protocol to quantitatively analyse the virus invasion efficiency.","PeriodicalId":23747,"journal":{"name":"Virulence","volume":null,"pages":null},"PeriodicalIF":5.5,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11197921/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141443402","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Screening optimal DC-targeting peptide to enhance the immune efficacy of recombinant Lactobacillus expressing RHDV VP60. 筛选最佳直流电靶向肽，提高表达 RHDV VP60 的重组乳杆菌的免疫功效。

IF 5.5 1区农林科学

Virulence Pub Date : 2024-12-01 Epub Date: 2024-06-20 DOI: 10.1080/21505594.2024.2368080

Tian Xia, Xiao Lu, Deming Kong, Tiantian Guo, Yueyi Gao, Lingxiang Xin, Yanping Jiang, Xiaona Wang, Zhifu Shan, Jiaxuan Li, Han Zhou, Wen Cui, Xinyuan Qiao, Lijie Tang, Yijing Li, Li Wang

{"title":"Screening optimal DC-targeting peptide to enhance the immune efficacy of recombinant Lactobacillus expressing RHDV VP60.","authors":"Tian Xia, Xiao Lu, Deming Kong, Tiantian Guo, Yueyi Gao, Lingxiang Xin, Yanping Jiang, Xiaona Wang, Zhifu Shan, Jiaxuan Li, Han Zhou, Wen Cui, Xinyuan Qiao, Lijie Tang, Yijing Li, Li Wang","doi":"10.1080/21505594.2024.2368080","DOIUrl":"10.1080/21505594.2024.2368080","url":null,"abstract":"Dendritic cells (DCs) present an ideal target for delivering immunogenic cargo due to their potent antigen-presenting capabilities. This targeting approach holds promise in vaccine development by enhancing the efficiency of antigen recognition and capture by DCs. To identify a high-affinity targeting peptide binding to rabbit DCs, rabbit monocyte-derived DCs (raMoDCs) were isolated and cultured, and a novel peptide, HS (HSLRHDYGYPGH), was identified using a phage-displayed peptide library. Alongside HS, two other DC-targeting peptides, KC1 and MY, previously validated in our laboratory, were employed to construct recombinant Lactgobacillus reuteri fusion-expressed rabbit hemorrhagic disease virus (RHDV) capsid protein VP60. These recombinant Lactobacillus strains were named HS-VP60/L. reuteri, KC1-VP60/L. reuteri, and MY-VP60/L. reuteri. The ability of these recombinant Lactobacillus to bind rabbit DCs was evaluated both in vivo and in vitro. Results demonstrated that the DC-targeting peptide KC1 significantly enhanced the capture efficiency of recombinant Lactobacillus by raMoDCs, promoted DC maturation, and increased cytokine secretion. Furthermore, oral administration of KC1-VP60/L. reuteri effectively induced SIgA and IgG production in rabbits, prolonged rabbit survival post-challenge, and reduced RHDV copies in organs. In summary, the DC-targeting peptide KC1 exhibited robust binding to raMoDCs, and recombinant Lactobacillus expressing KC1-VP60 protein antigens efficiently induced systemic and mucosal immune responses in rabbits, conferring protective efficacy against RHDV. This study offers valuable insights for the development of novel RHDV vaccines.","PeriodicalId":23747,"journal":{"name":"Virulence","volume":null,"pages":null},"PeriodicalIF":5.5,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11195490/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141427761","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0