Virulence最新文献_第5页

Physiological role of bicarbonate in microbes: A double-edged sword? 碳酸氢盐在微生物中的生理作用：一把双刃剑？

IF 5.5 1区农林科学

Virulence Pub Date : 2025-12-01 Epub Date: 2025-03-06 DOI: 10.1080/21505594.2025.2474865

Ashok Aspatwar, Jenny Parkkinen, Seppo Parkkila

{"title":"Physiological role of bicarbonate in microbes: A double-edged sword?","authors":"Ashok Aspatwar, Jenny Parkkinen, Seppo Parkkila","doi":"10.1080/21505594.2025.2474865","DOIUrl":"10.1080/21505594.2025.2474865","url":null,"abstract":"HCO3- is involved in pH homoeostasis and plays a multifaceted role in human health. HCO3- has been recognized for its antimicrobial properties and is pivotal in bacterial antibiotic susceptibility. Notably, the interconversion between CO2 and HCO3-, facilitated by the enzyme carbonic anhydrase (CA), is crucial in tissues infected by pathogens. Studies have highlighted the antimicrobial potency of CA inhibitors, emphasizing the importance of this enzyme in this area. The potential of HCO3- as an antibiotic adjuvant is evident; its ability to increase virulence in pathogens such as Enterococcus faecalis and Mycobacterium tuberculosis requires meticulous scrutiny. HCO3- modulates bacterial behaviours in diverse manners: it promotes Escherichia coli O157:H7 colonization in the human gut by altering specific gene expression and, with Pseudomonas aeruginosa, amplifies the effect of tobramycin on planktonic cells while promoting biofilm formation. These multifaceted effects necessitate profound mechanistic exploration before HCO3- can be considered a promising clinical adjuvant.","PeriodicalId":23747,"journal":{"name":"Virulence","volume":"16 1","pages":"2474865"},"PeriodicalIF":5.5,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11901407/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143568309","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Are Escherichia coli causing recurrent cystitis just ordinary uropathogenic E. coli (UPEC) strains? 引起复发性膀胱炎的大肠杆菌只是普通的尿路致病性大肠杆菌（UPEC）菌株吗？

IF 5.5 1区农林科学

Virulence Pub Date : 2025-12-01 Epub Date: 2024-12-26 DOI: 10.1080/21505594.2024.2444689

Nicolas Vautrin, Sandrine Dahyot, Marie Leoz, François Caron, Maxime Grand, Audrey Feldmann, François Gravey, Stéphanie Legris, David Ribet, Kévin Alexandre, Martine Pestel-Caron

{"title":"Are Escherichia coli causing recurrent cystitis just ordinary uropathogenic E. coli (UPEC) strains?","authors":"Nicolas Vautrin, Sandrine Dahyot, Marie Leoz, François Caron, Maxime Grand, Audrey Feldmann, François Gravey, Stéphanie Legris, David Ribet, Kévin Alexandre, Martine Pestel-Caron","doi":"10.1080/21505594.2024.2444689","DOIUrl":"https://doi.org/10.1080/21505594.2024.2444689","url":null,"abstract":"Specific determinants associated with Uropathogenic Escherichia coli (UPEC) causing recurrent cystitis are still poorly characterized. Using strains from a previous clinical study (Vitale study, clinicaltrials.gov, identifier NCT02292160) the aims of this study were (i) to describe genomic and phenotypic traits associated with recurrence using a large collection of recurrent and paired sporadic UPEC isolates and (ii) to explore within-host genomic adaptation associated with recurrence using series of 2 to 5 sequential UPEC isolates. Whole genome comparative analyses between 24 recurrent cystitis isolates (RCIs) and 24 phylogenetically paired sporadic cystitis isolates (SCIs) suggested a lower prevalence of putative mobile genetic elements (MGE) in RCIs, such as plasmids and prophages. The intra-patient evolution of the 24 RCI series over time was characterized by SNP occurrence in genes involved in metabolism or membrane transport and by plasmid loss in 5 out of the 24 RCI series. Genomic evolution occurred early in the course of recurrence, suggesting rapid adaptation to strong selection pressure in the urinary tract. However, RCIs did not exhibit specific virulence factor determinants and could not be distinguished from SCIs by their fitness, biofilm formation, or ability to invade HTB-9 bladder epithelial cells. Taken together, these results suggest a rapid but not convergent adaptation of RCIs that involves both strain- and host-specific characteristics.","PeriodicalId":23747,"journal":{"name":"Virulence","volume":"16 1","pages":"2444689"},"PeriodicalIF":5.5,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142898399","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bacterial metabolism in the host and its association with virulence. 宿主的细菌代谢及其与毒力的关系。

IF 5.5 1区农林科学

Virulence Pub Date : 2025-12-01 Epub Date: 2025-01-31 DOI: 10.1080/21505594.2025.2459336

Amrita Bhagwat, Tiyasa Haldar, Poonam Kanojiya, Sunil D Saroj

{"title":"Bacterial metabolism in the host and its association with virulence.","authors":"Amrita Bhagwat, Tiyasa Haldar, Poonam Kanojiya, Sunil D Saroj","doi":"10.1080/21505594.2025.2459336","DOIUrl":"10.1080/21505594.2025.2459336","url":null,"abstract":"The host restricted pathogens are competently dependent on their respective host for nutritional requirements. The bacterial metabolic pathways are surprisingly varied and remarkably flexible that in turn help them to successfully overcome competition and colonise their host. The metabolic adaptation plays pivotal role in bacterial pathogenesis. The understanding of host-pathogen metabolic crosstalk needs to be prioritized to decipher host-pathogen interactions. The review focuses on various aspects of host pathogen interactions that majorly involves adaptation of bacterial metabolism to counteract immune mechanisms by rectifying metabolic cues that provides pathogen the idea of different anatomical sites and the local physiology of the host. The key set of metabolites that are recognized as centre of competition between host and its pathogens are also briefly discussed. The factors that control the timely expression of virulence of bacterial pathogens is poorly understood. The perspective presented herein will facilitate us with a broader view of molecular mechanisms that modulates the expression of virulence factors in bacterial pathogens. The knowledge of crosslinked metabolic pathways of bacteria and their host will serve to develop novel potential therapeutics.","PeriodicalId":23747,"journal":{"name":"Virulence","volume":"16 1","pages":"2459336"},"PeriodicalIF":5.5,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11792850/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143075805","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Gut microbiome composition and metabolic activity in metabolic-associated fatty liver disease. 代谢相关脂肪性肝病的肠道微生物组成和代谢活性

IF 5.5 1区农林科学

Virulence Pub Date : 2025-12-01 Epub Date: 2025-03-28 DOI: 10.1080/21505594.2025.2482158

Daya Zhang, Qi Wang, Da Li, Shiju Chen, Jinrun Chen, Xuli Zhu, Feihu Bai

{"title":"Gut microbiome composition and metabolic activity in metabolic-associated fatty liver disease.","authors":"Daya Zhang, Qi Wang, Da Li, Shiju Chen, Jinrun Chen, Xuli Zhu, Feihu Bai","doi":"10.1080/21505594.2025.2482158","DOIUrl":"10.1080/21505594.2025.2482158","url":null,"abstract":"Metabolic Associated Fatty Liver Disease (MAFLD) impacts approximately 25% of the global population. Between April 2023 and July 2023, 60 patients with MAFLD, along with 60 age, ethnicity, and sex-matched healthy controls (HCs), were enrolled from the Inner Mongolia Autonomous Region, China. Analysis of gut microbiota composition and plasma metabolic profiles was conducted using metagenome sequencing and LC-MS. LEfSe analysis identified five pivotal species: Eubacterium rectale, Dialister invisus, Pseudoruminococcus massiliensis, GGB3278 SGB4328, and Ruminococcaceae bacteria. In subgroup analysis, Eubacterium rectale tended to increase by more than 2 times and more than double in the non-obese MAFLD group, and MAFLD with moderate hepatic steatosis (HS), respectively. Plasma samples identified 172 metabolites mainly composed of fatty acid metabolites such as propionic acid and butyric acid analogues. Ruminococcaceae bacteria have a strong positive correlation with β-alanine, uric acid, and L-valine. Pseudoruminococcus massiliensis has a strong positive correlation with β-alanine. Combinations of phenomics and metabolomics yielded the highest accuracy (AUC = 0.97) in the MAFLD diagnosis. Combinations of phenomics and metagenomics yielded the highest accuracy (AUC = 0.94) in the prediction of the MAFLD HS progress. Increases in Eubacterium rectale and decreases in Dialister invisus seem to be indicative of MAFLD patients. Eubacterium rectale may predict HS degree of MAFLD and play an important role in the development of non-obese MAFLD. Eubacterium rectale can generate more propionic acid and butyric acid analogues to absorb energy and increase lipid synthesis and ultimately cause MAFLD.","PeriodicalId":23747,"journal":{"name":"Virulence","volume":" ","pages":"2482158"},"PeriodicalIF":5.5,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11959907/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143693589","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

UvCYP503 is required for stress response and pathogenicity in Ustilaginoidea virens. UvCYP503是野田鼠的应激反应和致病性所必需的。

IF 5.5 1区农林科学

Virulence Pub Date : 2025-12-01 Epub Date: 2025-03-09 DOI: 10.1080/21505594.2025.2472877

Xiuxiu Cao, Hui Wen, Dagang Tian, Huanbin Shi, Kabin Xie, Jiehua Qiu, Yanjun Kou

{"title":"UvCYP503 is required for stress response and pathogenicity in Ustilaginoidea virens.","authors":"Xiuxiu Cao, Hui Wen, Dagang Tian, Huanbin Shi, Kabin Xie, Jiehua Qiu, Yanjun Kou","doi":"10.1080/21505594.2025.2472877","DOIUrl":"10.1080/21505594.2025.2472877","url":null,"abstract":"The fungus Ustilaginoidea virens, which impacts rice spikes, causes rice false smut (RFS), a significant prevalent disease in rice cultivation regions globally. Cytochrome P450 genes are known to be involved in secondary metabolism and pathogenesis in various species, but studies on CYP450 genes in U. virens are limited. In this research, a P450 family gene, CYP503, was found up-regulated during invasion stage of U. virens. Observation of fluorescence indicated that UvCYP503-GFP is situated within cytoplasm of hyphae. Disruption of CYP503 led to decreased hyphal development, conidiation, and pathogenicity. Additional RNA-seq assay revealed that UvCYP503 affects the transcript of genes associated with pathogenicity, various stress responses, and other CYP450 genes. In alignment with RNA-seq results, compared with wild-type, ΔUvcyp503 mutants showed increased sensitivity to cell wall stresses, but reduced sensitivity to osmotic and hyperosmotic stressors. Moreover, ΔUvcyp503 mutants exhibited decreased sensitivity to the fungicides difenoconazole and tebuconazole. This study represents a phenome-based functional analysis of a CYP503 gene in U. virens and provides valuable genetic resources for further research in filamentous fungi and other plant pathogens.","PeriodicalId":23747,"journal":{"name":"Virulence","volume":" ","pages":"2472877"},"PeriodicalIF":5.5,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11901397/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143543726","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Chemosensory protein 16 has an immune function and participates in host-pathogen interaction in Galleria mellonella infected with Pseudomonas entomophila. 化学感觉蛋白16在嗜虫假单胞菌感染的mellonia中具有免疫功能并参与宿主-病原体相互作用。

IF 5.5 1区农林科学

Virulence Pub Date : 2025-12-01 Epub Date: 2025-02-28 DOI: 10.1080/21505594.2025.2471367

Jakub Kordaczuk, Michał Sułek, Paweł Mak, Alicja Frączek, Iwona Wojda

{"title":"Chemosensory protein 16 has an immune function and participates in host-pathogen interaction in Galleria mellonella infected with Pseudomonas entomophila.","authors":"Jakub Kordaczuk, Michał Sułek, Paweł Mak, Alicja Frączek, Iwona Wojda","doi":"10.1080/21505594.2025.2471367","DOIUrl":"10.1080/21505594.2025.2471367","url":null,"abstract":"Chemosensory protein 16 was identified in the hemolymph of Galleria mellonella as a protein with an amount increasing after oral infection with 10^3 CFU of Pseudomonas entomophila, and decreasing after infection with a higher dose (10^5 CFU) of bacteria. The expression of the CSP16 gene occurred in the fat body and in the gut and correlated with changes in the protein level in the hemolymph. The CSP16 protein inhibited P. entomophila growth in the concentration range from 0.15 to 6 nM. Additionally, the CSP16 protein showed bactericidal activity against P. entomophila, Bacillus thuringiensis, and Escherichia coli in the range of 2-18 μM, but only in the presence of protease inhibitors, otherwise it was degraded by extracellular proteases secreted by P. entomophila. We demonstrated that the bactericidal activity of CSP16 was related to its ability to perforate bacterial cellular membranes in a dose-dependent manner. The antimicrobial properties of this protein were also confirmed with the use of Atomic Force Microscopy, which showed significant changes in the topology of different bacterial cell surfaces. Finally, when CSP16 was injected in vivo into G. mellonella larvae one hour after infection with P. entomophila, more survivors were observed at particular time-points. Taking into account its immune properties and putative ability to bind bacteria-derived compounds, the possible function of CSP16 in the host-pathogen interaction is discussed.","PeriodicalId":23747,"journal":{"name":"Virulence","volume":"16 1","pages":"2471367"},"PeriodicalIF":5.5,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11875508/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143524737","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Assessment for antibiotic resistance in Helicobacter pylori: A practical and interpretable machine learning model based on genome-wide genetic variation. 幽门螺杆菌抗生素耐药性评估：基于全基因组遗传变异的实用且可解释的机器学习模型。

IF 5.5 1区农林科学

Virulence Pub Date : 2025-12-01 Epub Date: 2025-03-21 DOI: 10.1080/21505594.2025.2481503

Yingying Wang, Shuwen Zheng, Rui Guo, Yanke Li, Honghao Yin, Xunan Qiu, Jijun Chen, Chuxuan Ni, Yuan Yuan, Yuehua Gong

{"title":"Assessment for antibiotic resistance in Helicobacter pylori: A practical and interpretable machine learning model based on genome-wide genetic variation.","authors":"Yingying Wang, Shuwen Zheng, Rui Guo, Yanke Li, Honghao Yin, Xunan Qiu, Jijun Chen, Chuxuan Ni, Yuan Yuan, Yuehua Gong","doi":"10.1080/21505594.2025.2481503","DOIUrl":"10.1080/21505594.2025.2481503","url":null,"abstract":"Helicobacter pylori (H. pylori) antibiotic resistance poses a global health threat. Accurate identification of antibiotic resistant strains is essential for the control of infection. In the present study, our goal is to leverage the whole-genome data of H. pylori to develop practical and interpretable machine learning (ML) models for comprehensive antibiotic resistance assessment. A total of 296 H. pylori isolates with genome-wide data were downloaded from the Bacterial and Viral Bioinformatics Resource Center (BV-BRC) and the National Center for Biotechnology Information (NCBI) databases. By training ML models on feature sets of single nucleotide polymorphisms from SNP calling (SNPs-1), antibiotic-resistance SNP annotated by the Comprehensive Antibiotic Resistance Database (SNPs-2), gene presence or absence (GPA), we generated predictive models for four antibiotics and multidrug-resistance (MDR). Among them, the models that combined SNPs-1, SNPs-2, and GPA data demonstrated the best performance, with the eXtreme Gradient Boosting (XGBoost) consistently outperforming others. And then we utilized the SHapley Additive exPlanations (SHAP) method to interpret the ML models. Furthermore, a free web application for the MDR model was deployed to the GitHub repository (https://H.pylori/MDR/App/). Our study demonstrated the promise of employing whole-genome data in conjunction with ML algorithms to forecast H. pylori antibiotic resistance. In the future, the application of this approach for predicting H. pylori antibiotic resistance would hold the potential to mitigate the empiric administration.","PeriodicalId":23747,"journal":{"name":"Virulence","volume":"16 1","pages":"2481503"},"PeriodicalIF":5.5,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11934168/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143677337","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Multifaceted quorum-sensing inhibiting activity of 3-(Benzo[d][1,3]dioxol-4-yl)oxazolidin-2-one mitigates Pseudomonas aeruginosa virulence. 3-(苯并[d][1,3]二恶茂-4-基)恶唑烷-2-酮的多方面法定人数感应抑制活性可减轻铜绿假单胞菌的毒性。

IF 5.5 1区农林科学

Virulence Pub Date : 2025-12-01 Epub Date: 2025-03-19 DOI: 10.1080/21505594.2025.2479103

Yi Wu, Fulong Wen, Shiyi Gou, Qiman Ran, Yiwen Chu, Wenbo Ma, Kelei Zhao

{"title":"Multifaceted quorum-sensing inhibiting activity of 3-(Benzo[d][1,3]dioxol-4-yl)oxazolidin-2-one mitigates Pseudomonas aeruginosa virulence.","authors":"Yi Wu, Fulong Wen, Shiyi Gou, Qiman Ran, Yiwen Chu, Wenbo Ma, Kelei Zhao","doi":"10.1080/21505594.2025.2479103","DOIUrl":"https://doi.org/10.1080/21505594.2025.2479103","url":null,"abstract":"As antibiotic resistance escalates into a global health crisis, novel therapeutic approaches against infectious diseases are in urgent need. Pseudomonas aeruginosa, an adaptable opportunistic pathogen, poses substantial challenges in treating a range of infections. The quorum-sensing (QS) system plays a pivotal role in orchestrating the production of a large set of virulence factors in a cell density-dependent manner, and the anti-virulence strategy targeting QS may show huge potential. Here, we present a comprehensive investigation into the potential of the synthesized compound 3-(benzo[d][1,3]dioxol-4-yl)oxazolidin-2-one (OZDO, C10H9NO4) as a QS inhibitor to curb the virulence of P. aeruginosa. By employing an integrated approach encompassing in silico screening, in vitro and in vivo functional identification, we elucidated the multifaceted effects of OZDO. Molecular docking predicted that OZDO interfered with three core regulatory proteins of P. aeruginosa QS system. Notably, OZDO exhibited significant inhibition on the production of pyocyanin, rhamnolipid and extracellular proteases, biofilm formation, and cell motilities of P. aeruginosa. Transcriptomic analysis and quantitative real-time PCR displayed the down-regulation of QS-controlled genes in OZDO-treated PAO1, reaffirming the QS-inhibition activity of OZDO. In vivo assessments using a Caenorhabditis elegans-infection model demonstrated OZDO mitigated P. aeruginosa pathogenicity, particularly against the hypervirulent strain PA14. Moreover, OZDO in combination with polymyxin B and aztreonam presented a promising avenue for innovative anti-infective therapy. Our study sheds light on the multifaceted potential of OZDO as an anti-virulence agent and its significance in combating P. aeruginosa-associated infections.","PeriodicalId":23747,"journal":{"name":"Virulence","volume":"16 1","pages":"2479103"},"PeriodicalIF":5.5,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143658883","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Clinical analysis and risk factors associated with poor prognosis in nontuberculous mycobacterial infection. 非结核性分枝杆菌感染预后不良的临床分析及相关危险因素。

IF 5.5 1区农林科学

Virulence Pub Date : 2025-12-01 Epub Date: 2025-02-03 DOI: 10.1080/21505594.2025.2459313

Jinjing Chai, Sujie Zhang, Chong Ma, Qimin Mei, Tao Liu, Jihai Liu, Yecheng Liu, Huadong Zhu

{"title":"Clinical analysis and risk factors associated with poor prognosis in nontuberculous mycobacterial infection.","authors":"Jinjing Chai, Sujie Zhang, Chong Ma, Qimin Mei, Tao Liu, Jihai Liu, Yecheng Liu, Huadong Zhu","doi":"10.1080/21505594.2025.2459313","DOIUrl":"10.1080/21505594.2025.2459313","url":null,"abstract":"Recently, the incidence and prevalence of NTM have been increasing nationwide in many countries. This study aimed to identify risk factors associated with the prognosis and mortality of non-HIV nontuberculous mycobacterial disease patients. This retrospective study was conducted at Peking Union Medical College Hospital. The electronic medical records in the hospital's database from January 2013 to December 2022 were retrospectively reviewed. Relevant data, including clinical characteristics, laboratory findings, microbiological tests, treatments, and outcomes were collected and subjected to statistical analyses. The search identified 745 patients diagnosed with NTM infection, of whom 147 met the inclusion criteria. NTM pulmonary disease was the most commonly observed (n = 93; 63.3%), followed by disseminated infection (n = 43; 29.3%). The most frequent NTM species was Mycobacterium avium complex (55.8%), followed by Mycobacterium abscessus (21.2%). The incidence of Aspergillus and Pseudomonas aeruginosa infection was significantly higher in the NTM pulmonary disease group than in the disseminated NTM group. Cumulative mortality in the total patients was 24.49% at 5 years. High Charlson Comorbidity Index (CCI), high neutrophil-to-lymphocyte-ratio (NLR), haematological disease, and disseminated infection were identified as independent predictors of unfavourable outcomes. The area under the curve (AUC) values for NLR and neutrophil-to-monocyte-plus-lymphocyte-ratio (NMLR) were 0.751 and 0.763 with optimal cut-off values of 9.50 and 3.83, respectively, for prediction of mortality in patients with NTM disease.","PeriodicalId":23747,"journal":{"name":"Virulence","volume":"16 1","pages":"2459313"},"PeriodicalIF":5.5,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11792823/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143080926","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effect of COVID-19 infection on thyroid function status and clinical indexes among hypothyroid outpatients. COVID-19感染对甲状腺功能减退门诊患者甲状腺功能状况及临床指标的影响

IF 5.5 1区农林科学

Virulence Pub Date : 2025-12-01 Epub Date: 2024-12-27 DOI: 10.1080/21505594.2024.2441397

Bingxin Li, Xiaoyun Feng, Yihan Zhang, Yunhong Huang, Mingyu Gu, Haiyan Sun, Wenqian Ren, Qin Zhen, Tingting Shen, Ling Pan, Tingting Fan, Qin Qin, Fang Liu, Yongde Peng, Yufan Wang, Huanbai Xu

{"title":"Effect of COVID-19 infection on thyroid function status and clinical indexes among hypothyroid outpatients.","authors":"Bingxin Li, Xiaoyun Feng, Yihan Zhang, Yunhong Huang, Mingyu Gu, Haiyan Sun, Wenqian Ren, Qin Zhen, Tingting Shen, Ling Pan, Tingting Fan, Qin Qin, Fang Liu, Yongde Peng, Yufan Wang, Huanbai Xu","doi":"10.1080/21505594.2024.2441397","DOIUrl":"https://doi.org/10.1080/21505594.2024.2441397","url":null,"abstract":"The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) spread rapidly, leading to an Omicron outbreak in Shanghai in mid-December after adjustments to the Coronavirus Disease 2019 (COVID-19) control strategy. To investigate the impact of COVID-19 infection among hypothyroid patients, we gathered data on the hypothyroid outpatients with COVID-19 infection during this time at the Thyroid Disease Center (TDC) of Shanghai Central Hospital. Patients were divided into two groups based on whether their hypothyroidism was caused by Hashimoto's Thyroiditis (HT): the HT and the non-HT group. We assessed the differences between pre-infection and clinical follow-up at one month (day (D) 30) and three months (D90) after COVID-19 infection. In HT group, thyroid-stimulating hormone (TSH) levels decreased significantly compared to pre-infection levels (p = 0.013), while free triiodothyronine (FT3) levels increased at D90 compared to both D30 post-infection and pre-infection levels (p < 0.001 and p = 0.005). Hemoglobin levels also increased after COVID-19 infection (p = 0.033). For non-HT patients, FT3 levels increased at D30 compared to pre-infection levels (p = 0.017). Moreover, inactivated SARS-CoV-2 vaccination can preserve thyroid function stability in patients with hypothyroidism.","PeriodicalId":23747,"journal":{"name":"Virulence","volume":"16 1","pages":"2441397"},"PeriodicalIF":5.5,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142898401","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0