Virulence最新文献_第4页

Pathological protection and molecular regulation of cystatin B in Nile tilapia (Oreochromis niloticus) bacterial disease. 半胱抑素B在尼罗罗非鱼细菌性疾病中的病理保护作用及分子调控作用。

IF 5.4 1区农林科学

Virulence Pub Date : 2025-12-01 Epub Date: 2025-09-29 DOI: 10.1080/21505594.2025.2563010

Yongxiong Huang, Xuyan Tan, Yuwen Li, Zhiqiang Zhang, Yu Huang, Jichang Jian

{"title":"Pathological protection and molecular regulation of cystatin B in Nile tilapia (Oreochromis niloticus) bacterial disease.","authors":"Yongxiong Huang, Xuyan Tan, Yuwen Li, Zhiqiang Zhang, Yu Huang, Jichang Jian","doi":"10.1080/21505594.2025.2563010","DOIUrl":"10.1080/21505594.2025.2563010","url":null,"abstract":"Cystatin B (CSTB) is an endogenous cysteine protease inhibitor that plays a critical role in the modulation of numerous biological processes, involving immune responses, apoptosis, and inflammation in mammals. However, its immunological functions in teleost, particularly Nile tilapia (Oreochromis niloticus) remain unclear. In the present study, the CSTB was cloned and characterized from Nile tilapia (On-CSTB) and its role in bacterial infection was revealed. The On-CSTB open reading frame is 300 bp encoding 99 amino acids that contains a conserved cystatin domain. Multiple sequence alignment analysis revealed that On-CSTB share over 75% identity with fish lineages, and 45% identity with mammals. qPCR analysis showed that On-CSTB is widely expressed across various tissues and highly expressed in blood cells and intestines and can be significantly inducted by Streptococcus agalactiae (S. agalactiae) and Aeromonas hydrophila (A. hydrophila). In vivo experiments demonstrated that On-CSTB protein could suppress inflammation while participating in the MyD88/NF-κB signaling pathway and inflammasome activation, affecting apoptosis and pyroptosis processes. Moreover, On-CSTB contributed to enhanced tissue integrity and alleviated pathological damage. These findings collectively highlight On-CSTB as a crucial immunomodulator that contributes to host defense and tissue protection in fish. The present study offers novel perspectives on the immunomodulatory role of CSTB in tilapia, providing a basis for disease resistance strategies in aquaculture.","PeriodicalId":23747,"journal":{"name":"Virulence","volume":"16 1","pages":"2563010"},"PeriodicalIF":5.4,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12482433/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145186543","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Antifungal mechanism of ketone volatile organic compounds against Pseudogymnoascus destructans. 酮类挥发性有机化合物对刺槐的抑菌机理研究。

IF 5.4 1区农林科学

Virulence Pub Date : 2025-12-01 Epub Date: 2025-10-07 DOI: 10.1080/21505594.2025.2569627

Yaping Lu, Yue Zhu, Long Huang, Yingting Pu, Xiaoyu Sun, Jiang Feng, Keping Sun

{"title":"Antifungal mechanism of ketone volatile organic compounds against Pseudogymnoascus destructans.","authors":"Yaping Lu, Yue Zhu, Long Huang, Yingting Pu, Xiaoyu Sun, Jiang Feng, Keping Sun","doi":"10.1080/21505594.2025.2569627","DOIUrl":"10.1080/21505594.2025.2569627","url":null,"abstract":"Ketone volatile organic compounds have demonstrated favorable inhibitory activity against a wide range of pathogenic fungi, including Pseudogymnoascus destructans (Pd), the lethal pathogenic fungus responsible for white-nose syndrome in bats. However, the mechanism of fungal inhibition by ketones remains unclear. In this study, we employed transcriptomic analysis to conduct RNA sequencing on Pd treated with 2-undecanone and 2-nonanone, aiming to investigate the effects of these ketones on the gene expression profiles of Pd. The results indicated that 2-undecanone and 2-nonanone inhibit spore germination in Pd and cause significant damage to its mycelium. The minimum inhibitory concentrations (MIC) were determined to be 25.94 μg/mL and 135.41 μg/mL, respectively. Transcriptomic analysis revealed these ketones affects Pd through multiple pathways, inducing lesions in the cell wall and membrane, and disrupting ribosomal stability by interfering with rRNA modifications and ribosome assembly. Additionally, we found that 2-undecanone impacts enzymes involved in the tricarboxylic acid cycle, disrupting energy metabolism by interfering with critical metabolic pathways in aerobic organisms. In contrast, 2-nonanone directly damages Pd DNA, triggering the activation of DNA damage repair mechanisms. This study provides a theoretical basis for exploring novel antifungal strategies targeting Pd, suggesting that ketones may serve as potential in vitro defense and control tools, laying the groundwork for the subsequent development of efficient fumigants.","PeriodicalId":23747,"journal":{"name":"Virulence","volume":"16 1","pages":"2569627"},"PeriodicalIF":5.4,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12505510/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145239834","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Future disease burden due to the rise of emerging infectious disease secondary to climate change may be being under-estimated. 由于气候变化继发的新发传染病增加而造成的未来疾病负担可能被低估了。

IF 5.5 1区农林科学

Virulence Pub Date : 2025-12-01 Epub Date: 2025-05-11 DOI: 10.1080/21505594.2025.2501243

Paul R Hunter

引用次数: 0

Isopropyl paraben targets type III secretion to inhibit Salmonella enterica serovar typhimurium infection. 对羟基苯甲酸异丙酯靶向III型分泌抑制肠沙门氏菌血清型鼠伤寒杆菌感染。

IF 5.4 1区农林科学

Virulence Pub Date : 2025-12-01 Epub Date: 2025-08-26 DOI: 10.1080/21505594.2025.2548621

Jinli Ge, Xueyu Li, Qian Lu, Siqi Li, Jiayang Liu, Xuming Deng, Hongtao Liu, Jiazhang Qiu

{"title":"Isopropyl paraben targets type III secretion to inhibit Salmonella enterica serovar typhimurium infection.","authors":"Jinli Ge, Xueyu Li, Qian Lu, Siqi Li, Jiayang Liu, Xuming Deng, Hongtao Liu, Jiazhang Qiu","doi":"10.1080/21505594.2025.2548621","DOIUrl":"10.1080/21505594.2025.2548621","url":null,"abstract":"Salmonella enterica, a food- and water-borne pathogen, triggers food poisoning and enteric infections. The effectiveness of antibiotics against Salmonella infections is decreasing due to bacterial resistance. Developing novel antimicrobial agents is crucial and urgent. Here, we screened 550 natural compounds and found that isopropyl paraben (IPPB) effectively inhibited Salmonella enterica serovar Typhimurium (ST) invasion of host cells without impacting bacterial growth, reducing the risk of developing bacterial resistance. Further investigation revealed that IPPB bound to the regulatory protein HilD and inhibited the transcription of the type III protein secretion system 1 (T3SS-1) regulatory genes hilD, hilC and rtsA. This interference blocked the production of T3SS-1 effectors. Importantly, IPPB exerted certain therapeutic effects on both G. mellonella larvae and murine models infected with ST and improved the ST-induced gut microbiota dysbiosis in mice. These findings suggest that IPPB has potential as a novel antimicrobial agent, targeting the T3SS-1 of Salmonella.","PeriodicalId":23747,"journal":{"name":"Virulence","volume":" ","pages":"2548621"},"PeriodicalIF":5.4,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12382468/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144875460","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of Isaria cateniannulata on the colonization process and enzyme activity of Fagopyrum tataricum seeds during germination. 钩藤对苦荞种子萌发定殖过程及酶活性的影响。

IF 5.4 1区农林科学

Virulence Pub Date : 2025-12-01 Epub Date: 2025-08-15 DOI: 10.1080/21505594.2025.2543062

Xiaona Zhang, Lingdi Gu, Guimin Yang, Can Liu, Kaifeng Huang, Qingfu Chen

{"title":"Effects of Isaria cateniannulata on the colonization process and enzyme activity of Fagopyrum tataricum seeds during germination.","authors":"Xiaona Zhang, Lingdi Gu, Guimin Yang, Can Liu, Kaifeng Huang, Qingfu Chen","doi":"10.1080/21505594.2025.2543062","DOIUrl":"10.1080/21505594.2025.2543062","url":null,"abstract":"Endophytic colonization of entomopathogenic fungi has garnered significant attention for its role in promoting plant growth. Specifically, Isaria cateniannulata has shown a positive effect on the germination of Fagopyrum tataricum (buckwheat) seeds, but the mechanisms underlying this promotion remain unclear. This study aims to elucidate the colonization process of I. cateniannulata in F. tataricum seeds during germination stages, quantify the colonization efficiency and tissue specificity of the fungus, and investigate the temporal dynamics of antioxidant enzyme activities and malondialdehyde content triggered by fungal colonization. Furthermore, we evaluated the potential of I. cateniannulata-colonized seedlings to suppress T. urticae populations through oviposition inhibition. The results demonstrated for the first time that I. cateniannulata could successfully colonize germinating F. tataricum seeds during the seed imbibition and germination stages, either by forming dissolution zones with its spores or by germinating and forming mycelia. Initial colonization of all tissues was observed within 16 h, with colonization rates peaking after 5 d, with a preferential colonization rate observed as endosperm > embryo > seed coat. Furthermore, the colonization by I. cateniannulata enhanced peroxidase (POD) activity in the embryo and reduced malondialdehyde (MDA) content. Seedlings grown after colonization were also found to effectively reduce the number of eggs laid by T. urticae. These findings provide both theoretical insights and practical foundations for developing a symbiotic system between I. cateniannulata and F. tataricum seeds.","PeriodicalId":23747,"journal":{"name":"Virulence","volume":" ","pages":"2543062"},"PeriodicalIF":5.4,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12360201/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144776305","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Identification of fecal microbes as potential biomarkers for early diagnosis of fatty liver disease in cattle. 鉴定粪便微生物作为早期诊断牛脂肪肝的潜在生物标志物。

IF 5.4 1区农林科学

Virulence Pub Date : 2025-12-01 Epub Date: 2025-07-09 DOI: 10.1080/21505594.2025.2530166

Haoming Sun, Tingjun Liu, Xuyang Song, Sadiq M S Shah, Qin Zhang, Kerong Shi

{"title":"Identification of fecal microbes as potential biomarkers for early diagnosis of fatty liver disease in cattle.","authors":"Haoming Sun, Tingjun Liu, Xuyang Song, Sadiq M S Shah, Qin Zhang, Kerong Shi","doi":"10.1080/21505594.2025.2530166","DOIUrl":"10.1080/21505594.2025.2530166","url":null,"abstract":"Fatty liver disease is prevalent during parturition in dairy cattle. Therefore, there is an urgent need to develop novel, sensitive biomarkers for the early diagnosis of the metabolic disorders. Macroproteomics revealed that the faecal microbial community changes significantly when animal develops fatty liver disease. The microbial changes in cows with severe fatty liver (SFL) were greater than cows with moderate fatty liver (MFL) and normal condition (Norm). This suggests that microorganisms play an important role in the pathogenesis of metabolic disorders. In this study, faeces-sourced microorganisms and microbial proteins were identified and testified as novel biomarkers for the early diagnosis of fatty liver disease in cattle. For example, the AUC (area under curve) values, based on Receiver Operating Characteristics analysis, of using the combination of Lachnoanaerobaculum and Bifidobacterium (at the genus level) to discriminate MFL and SFL animals reached 0.944 and 0.867, respectively, and 0.922 and 0.985, respectively, for the combination of Bifidobacterium pseudolongum and Lachnospiraceae bacterium (at the species level). Interestingly, the differentially expressed microbial proteins are closely related to the identified microorganisms. For example, the majority of the top 20 microbial proteins with significant expression differences were derived from Bifidobacterium pseudolongum. Bifidobacterium pseudolongum was considered a prominent potential biomarker for the diagnosis of metabolic disorders, especially in fatty liver cattle. The results of this study confirm that faecal microbial dysbiosis signatures can serve as a diagnosis biomarker for non-alcoholic fatty liver disease (NAFLD), but also shed light on faecal microbiota transfer (FMT) experiments in treating NAFLD.","PeriodicalId":23747,"journal":{"name":"Virulence","volume":"16 1","pages":"2530166"},"PeriodicalIF":5.4,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12247111/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144601676","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A comprehension and systematic insight into the interaction between ferroptosis and virus infection: The implications of mechanisms and strategies. 对铁下垂和病毒感染之间相互作用的理解和系统见解：机制和策略的含义。

IF 5.4 1区农林科学

Virulence Pub Date : 2025-12-01 Epub Date: 2025-07-14 DOI: 10.1080/21505594.2025.2532806

Sai Niu, Yian Deng, Yonggen Yang, Junjie Wang, Chunyue Fang, Ying Zhou, Hanchuan Dai

{"title":"A comprehension and systematic insight into the interaction between ferroptosis and virus infection: The implications of mechanisms and strategies.","authors":"Sai Niu, Yian Deng, Yonggen Yang, Junjie Wang, Chunyue Fang, Ying Zhou, Hanchuan Dai","doi":"10.1080/21505594.2025.2532806","DOIUrl":"10.1080/21505594.2025.2532806","url":null,"abstract":"Ferroptosis is a novel form of iron-dependent programmed cell death characterized by iron metabolic derangement, an abnormal antioxidant system, and lipid peroxidation. Emerging evidence revealed that viruses modulate ferroptosis to facilitate their replication, dissemination, and pathogenesis, thereby promoting infection or achieving immune evasion by hijacking the host's iron metabolism. However, the interplay between ferroptosis and virus infections remains to be elucidated. This review comprehensively summarizes the core mechanisms of ferroptosis, including iron homeostasis, the system Xc-/GPX4 pathway, the FSP1/CoQ10 pathway, lipid peroxidation, and their essential roles were discussed in ferroptosis. We highlighted the relationship between the core mechanisms of ferroptosis and virus infection. Furthermore, we revealed the underlying pathogenic mechanisms of viral infections and the prospective applications targeting ferroptosis. This article is conducive to deepening our understanding of the regulatory mechanism of ferroptosis, unraveling the potential therapeutic intervention and pharmacological direction for the development of innovative ferroptosis-dependent antiviral agents.","PeriodicalId":23747,"journal":{"name":"Virulence","volume":"16 1","pages":"2532806"},"PeriodicalIF":5.4,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12269692/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144627268","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Circulated echovirus 18 strains in Guangdong Province and worldwide: A novel perspective on genetic diversity and recombination patterns. 在广东省和世界范围内流行的埃可病毒18株：遗传多样性和重组模式的新视角。

IF 5.4 1区农林科学

Virulence Pub Date : 2025-12-01 Epub Date: 2025-07-23 DOI: 10.1080/21505594.2025.2534519

Zhiyu Li, Xiaohan Yang, Ru Bai, Ping Li, Yanting Qin, Mingyong Luo

{"title":"Circulated echovirus 18 strains in Guangdong Province and worldwide: A novel perspective on genetic diversity and recombination patterns.","authors":"Zhiyu Li, Xiaohan Yang, Ru Bai, Ping Li, Yanting Qin, Mingyong Luo","doi":"10.1080/21505594.2025.2534519","DOIUrl":"10.1080/21505594.2025.2534519","url":null,"abstract":"Echovirus 18 (E18) has re-emerged as a global public health concern in recent years because of its association with severe neonatal systemic diseases that pose a risk of high mortality. The lack of effective intervention strategies for E18 infections is largely attributed to limited knowledge regarding molecular epidemiology and recombination patterns. In this study, we obtained seven full-length E18 sequences from infants in Guangdong Province and combined them with representative sequences from GenBank. Using this expanded dataset, we analysed the molecular epidemiological features, genetic characteristics, and recombination patterns of E18. Global statistics reveal a distinct double-peak pattern in the frequency of E18 infections throughout the year in the Northern Hemisphere. All strains isolated from clinical specimens were classified as genotype C2, which has emerged as the predominant circulating strain in Guangdong Province and globally. Several potential recombination events with E30 were identified among these seven strains, particularly in the P2 and P3 non-structural regions. This study offers new insights into the global dissemination, genetic diversity, and phylodynamics of E18, potentially providing valuable information for designing antiviral vaccines and the implementation of sustainable surveillance strategies to enhance virus prevention and control during public health crises.","PeriodicalId":23747,"journal":{"name":"Virulence","volume":" ","pages":"2534519"},"PeriodicalIF":5.4,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12296116/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144643652","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The highly pathogenic strain of porcine deltacoronavirus disrupts the intestinal barrier and causes diarrhea in newborn piglets. 猪三角冠状病毒的高致病性菌株破坏肠道屏障并引起新生仔猪腹泻。

IF 5.4 1区农林科学

Virulence Pub Date : 2025-12-01 Epub Date: 2025-01-06 DOI: 10.1080/21505594.2024.2446742

Xin Yao, Wei-Hong Lu, Wen-Ting Qiao, Yu-Qian Zhang, Bao-Ying Zhang, Hui-Xin Li, Jin-Long Li

{"title":"The highly pathogenic strain of porcine deltacoronavirus disrupts the intestinal barrier and causes diarrhea in newborn piglets.","authors":"Xin Yao, Wei-Hong Lu, Wen-Ting Qiao, Yu-Qian Zhang, Bao-Ying Zhang, Hui-Xin Li, Jin-Long Li","doi":"10.1080/21505594.2024.2446742","DOIUrl":"10.1080/21505594.2024.2446742","url":null,"abstract":"Porcine deltacoronavirus (PDCoV) is increasingly prevalent in newborn piglets with diarrhea. With the development of research on the virus and the feasibility of PDCoV cross-species transmission, the biosafety and the development of pig industry have been greatly affected. In this study, a PDCoV strain CH/LNFX/2022 was isolated from diarrheal newborn piglets at a farm in China. A genome-wide based phylogenetic analysis suggests that 97.5% to 99.2% homology existed in the whole genomes of other strains. Five amino acid mutations are seen for the first time in the S protein. By constructing 3D models, it was found that the S1-NTD/CTD and S2-HR-C regions produced structural alterations. Protein functional analysis showed that the structural changes of the three regions changed the epitope of S protein, the O-GalNAc glycosylation site and the 3C-like protease cleavage site. In addition, oral administration of 107 TCID50 CH/LNFX/2022 to newborn piglets successfully reproduced obvious clinical signs of piglets, such as diarrhea and dehydration. Meanwhile, PDCoV antigen was detected by immunofluorescence in the small intestine, and microscopic lesions and intestinal mucosal barrier destruction were detected by histological observation and scanning electron microscopy. Our study confirmed that porcine coronavirus strains increased pathogenicity through evolution, damaged the intestinal barrier of newborn piglets, and caused diarrhea in pigs. This study provided the candidate strains and theoretical basis for establishing the prevention and control system of vaccine and diagnostic methods for piglet diarrhea.","PeriodicalId":23747,"journal":{"name":"Virulence","volume":"16 1","pages":"2446742"},"PeriodicalIF":5.4,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142932791","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Emerging West African Genotype Chikungunya Virus in Mosquito Virome. 蚊子病毒中的新兴西非基孔肯雅病毒基因型。

IF 5.4 1区农林科学

Virulence Pub Date : 2025-12-01 Epub Date: 2024-12-23 DOI: 10.1080/21505594.2024.2444686

Pengpeng Xiao, Yujia Hao, Yuge Yuan, Wenzhou Ma, Yiquan Li, He Zhang, Nan Li

引用次数: 0