Journal of the Japan Society of the Reticuloendothelial System最新文献

{"title":"Plasma cell dyscrasias and normal plasma cells.","authors":"H. Harada, M. Kawano","doi":"10.3960/JSLRT1961.33.367","DOIUrl":"https://doi.org/10.3960/JSLRT1961.33.367","url":null,"abstract":"We have recently shown that two-color analysis with fluorescein isothiocyanate (FITC)-anti-CD38 antibody could clearly distinguish myeloma cells (plasma cells) from other hematopoietic cells in the bone marrow. Myeloma cells (plasma cells) alone were located at CD38strong positive(+++) fractions. We also identified two subpopulations among these myeloma cells: VLA-5-MPC-1- myeloma cells and VLA-5+MPC-1+ myeloma cells. Morphological examination showed that VLA-5- myeloma cells were mostly immature and VLA-5+ cells were mature myeloma cells. Furthermore, VLA-5- myeloma cells proliferated markedly in vitro and responded to interleukin 6 (IL-6), while VLA-5+ myeloma cells showed very low proliferation and no response to IL-6 but secreted higher amounts of M-protein in vitro significantly. Therefore, we could clarify heterogeneity of human myeloma cells in the bone marrow with regard to the expression of VLA-5; VLA-5- myeloma cells were proliferative immature cells and VLA-5+ cells were mature myeloma cells. To further distinguish normal plasma cells from mature myeloma cells phenotypically, we examined immunophenotypes of normal plasma cells and myeloma cells by two-color flow cytometry with FITC-anti-CD38 antibody and phycoerythrin (PE) staining with a given antibody. Normal plasma cells in the bone marrow, tonsil, spleen and lymph node were all CD19+CD56-. On the other hand, mature myeloma cells were mostly CD19- and most of them were CD56+, and there were no myeloma cell with the CD19+CD56- phenotype. According to this findings, we investigated the expression of CD19 and CD56 on plasma cells (CD38+++ fractions) in benign monoclonal gammopathy (BMG). Both CD19+CD56- and CD19-CD56+ plasma cells were detected, and were suggesting that BMG consisted of phenotypically normal plasma cells and myeloma cells. In order to investigate from where myeloma cells (plasma cells) originate, the phenotypes of B cell lineage in tonsils, lymph nodes and peripheral blood were analyzed by two-color analysis. In the tonsils and lymph nodes, germinal center B cells (GC-B cells) revealed CD38+VLA-5-MPC-1-CD24-CD10+ CD5-, while the phenotype of mantle zone B cells (MZ-B cells) were CD38-VLA-5+ MPC-1+CD24+CD10-CD5+. On the other hand, we found CD38moderately positive(++) cells in the peripheral blood and they revealed VLA-5-MPC-1-CD24-CD10+CD5-. Morphological examination showed that these cells were plasmacytoid. Therefore, we regarded the cells as precursor plasma cells. In conclusion, we could present here that 1) myeloma cells consisted of VLA-5- immature and VLA-5+ mature myeloma cells, 2) normal plasma cells were clearly distinguished from mature myeloma cells phenotypically, 3) plasma cells in BMG contained both normal plasma cells and myeloma cells, 4) precursor plasma cells were identified in the peripheral blood.","PeriodicalId":237003,"journal":{"name":"Journal of the Japan Society of the Reticuloendothelial System","volume":"25 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115431827","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Three cases of histiocytic sarcoma.","authors":"H. Sakamoto","doi":"10.3960/JSLRT1961.32.293","DOIUrl":"https://doi.org/10.3960/JSLRT1961.32.293","url":null,"abstract":"Three cases of histiocytic sarcoma were reported. Neoplastic cells in these cases posessed cytoplasm which was S100 protein and α1 antitrypsin positive but lysozyme negative, suggesting T zone histiocyte origin. Interdigitation was observed electron microscopically in case 2. However different results in immunohistochemical staining were observed among these cases. For example, LCA and OKT4 positive tumor cells were observed only in case 2 and Ki-1 positive cells in case 1 and 2. Erythrophagocytosis was observed only in tumor cells of case 3 obtained at autopsy. The patients were young (16, 48 and 19 years old respectively), and had multiple metastases involving extranodal organs.","PeriodicalId":237003,"journal":{"name":"Journal of the Japan Society of the Reticuloendothelial System","volume":"25 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"114504112","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"特発性血小板減少性紫斑病(ITP)と網内系","authors":"Hiroshi Iwasaki","doi":"10.3960/JSLRT1961.28.27","DOIUrl":"https://doi.org/10.3960/JSLRT1961.28.27","url":null,"abstract":"The spleens removed from 134 patients with chronic idiopathic thrombocytopenic purpura (ITP) treated in the 17 institutes in Japan were utilized in this study; the fresh surgical materials obtained in 18 of these cases were studied also by electron microscopy and immunohistochemistry. In ordinary HE sections, foamy macrophages were demonstrated in 55% of the 11 spleens from the patients without improvement after splenectomy, while the cells were found in only 25% of the 43 patients who revealed improved platelet counts after surgery. The foamy cells reacted strongly with a monoclonal antibody FU7 that was generated by using cultured malignant fibrous histiocytoma as an immunogen. When stained wite FU 7, there was a remarkable increase in the number of the recognizable foamy cells: 100% of the ITP spleens, and even 67% of the control spleens contained some positive cells. As to the subsets of T lymphocytes, the periarterial Iymphoid sheaths were rich in helper/inducer cells that outnumbered suppressor/cytotoxic cells. By electron microscopy, the ITP spleens showed pronounced phagocytosis of platelets by macrophages in the medullary cords and marginal zones; foamy macrophages were filled with numerous myelin figures and residual bodies probably deriving from incompletely digested platelet membrane. A mild increase of IgG-positive lymphoid cells and plasma cells in these regions, together with hyperplastic lymph follicles with prominent germinal centers in some cases, may represent the activated state of the B lymphocyte system in ITP. The follicles were generally atrophic and devoid of germinal centeres in the cases treated with prolonged steroid therapy.","PeriodicalId":237003,"journal":{"name":"Journal of the Japan Society of the Reticuloendothelial System","volume":"22 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115767447","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Immunohistochemical study of dendritic cells, macrophages and lymphocytes in gastric cancer.","authors":"H. Iwase, K. Morise","doi":"10.3960/JSLRT1961.32.235","DOIUrl":"https://doi.org/10.3960/JSLRT1961.32.235","url":null,"abstract":"Infiltration of dendritic cells, macrophages and lymphcytes into tumor tissues was immunohistochemically investigated using new monoclonal antibodies ID-1 for dendritic cells and CD68 for macrophages in 68 patients with gastric cancer. A serial section technique and double staining method were used for the phenotypic analysis. In the normal musosa, ID-1+ cells were located under the surface epithelium, while CD68+ cells were widely distributed in the lamina propria. Most of ID-1+ cells and CD68+ cells in the normal mucosa were negative for ICAM-1 and activated markers of CD25 and HLA-DR antigens. In cases of mucosal cancer, the numbers of ID-1+ cells and CD 68+ cells in the cancer tissue were similar to the normal mucosa. When cancer cells invaded the submucosa, ID-1+ cells and CD68+ cells were significantly increased in the cancer tissue except for the cases of signet ring cell carcinoma. The increased ID-1+ cells were positive for ICAM-1, HLA-DR, CD25 and CD11c, while most of CD68+ cells were negative for ICAM-1 and CD25 antigans. Further, ID-1+ cells were predominantly distributed adjacent to the tumor tissues with a marked infiltration of CD3+ cells. Conversely, CD68+ cells were distributed in the cancer stroma and necrotic tissues without CD3+ cell infiltration. These findings suggested that ID-1+ dendritic cells play a crucial role in the immune response agaist the cancer tissue.","PeriodicalId":237003,"journal":{"name":"Journal of the Japan Society of the Reticuloendothelial System","volume":"90 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"114707631","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ontogeny of the chicken macrophage.","authors":"K. Tajima, M. Yamakawa, Y. Imai","doi":"10.3960/JSLRT1961.30.253","DOIUrl":"https://doi.org/10.3960/JSLRT1961.30.253","url":null,"abstract":"","PeriodicalId":237003,"journal":{"name":"Journal of the Japan Society of the Reticuloendothelial System","volume":"41 8 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"126182550","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"T cell rich B cell lymphoma","authors":"孝一 大島, 淳司 鈴宮, 昌弘 菊池","doi":"10.3960/JSLRT1961.36.391","DOIUrl":"https://doi.org/10.3960/JSLRT1961.36.391","url":null,"abstract":"","PeriodicalId":237003,"journal":{"name":"Journal of the Japan Society of the Reticuloendothelial System","volume":"17 8 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"128183951","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"中国吉林省(白求恩医科大学)における非ホジキンリンパ腫の研究","authors":"雅潔 張, 昌宏 斉藤, 佳敬 上坂, 勤 綿貫","doi":"10.3960/JSLRT1961.33.185","DOIUrl":"https://doi.org/10.3960/JSLRT1961.33.185","url":null,"abstract":"A detailed histopathologic and immunohistochemical study has been carried out on primary extranodal non-Hodgkin's lymphoma (NHL), especially in gastrointestinal (GI) tract, and on primary intestinal and mesenteric lymph nodal lymphoma, comparatively. The material were collected from Jilin Provience, China. The results obtained were as follows:A total of 52 cases with extranodal NHL were consisted of 12 of stomach, 22 of intestine (16 of small intestine, 5 of ileocecum and 1 of cecum), 11 of Waldeyer's ring, and 7 of the others.Histologically, 45 cases were diffuse (D) type and the other 7 were follicular (F). D/F ratio was 6.4:1. Phenotypically, B/T ratio was 25:1.According to WF classification, the intermediate grade of malignancy was 43 cases (82.7%) including 24 cases (46.2%) of diffuse, large non-cleaved which was the most preponderant type.Five of 34 cases (14.7%) of primary GI lymphoma were reclassified as mocosa-associated lymphoid tissue (MALT) lymphoma. Three cases were composed of centrocyte-like (CCL) cells and the other 2 were of large blast-like cells. In all cases, the lymphoepithelial lesion (LEL) and reactive or neoplastic follicular structure were noted histologically, and positive finding of paranuclear dot for LN-1, both in CCL and blast-like cells, was found immunohistochemically.Two of 34 cases (5.9%) of primary GI lymphoma showed an appearance of multiple lymphomatous polyposis. The one was recognized as the results of dissemination of MALT lymphoma, because the LEL was distinctively exhibited and the medium-sized cell with nucleus showing marked cleavation. The other was identified as mantle zone lymphoma of multicentric origin, since the main component cells within the vague nodules were small and round nucleus with slight cleavation, negative for LN-1 and positive for LN-2, and no LEL was found.No evident association between the primary small intestinal and the primary mesenteric lymph nodal lymphoma had been recorded.","PeriodicalId":237003,"journal":{"name":"Journal of the Japan Society of the Reticuloendothelial System","volume":"33 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"126985885","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hematological disorders and human macrophages.","authors":"Kokichi Yamamoto, Y. Nakabo, N. Harakawa, M. Sasada","doi":"10.3960/JSLRT1961.33.245","DOIUrl":"https://doi.org/10.3960/JSLRT1961.33.245","url":null,"abstract":"We report the results of in vitro experiments and in vivo studies to explore the possibility whether macrophage function might influence on the prognosis of human leukemia. At first, we noticed a transient but remarkable monocytosis during recovery after intensive chemotherapy in peripheral blood of patients with acute leukemia. Such remarkable monocytosis was observed only when they achieved a complete hematological remission. This may reflect the recovery of normal hematopoiesis, in addition, monocytes might contribute to the reduction of residual leukemic cells. To explore such possibility, we took advantage of monocyte-derived macrophages (Mφ). Mφ, pretreated with IFN-γ or LPS, lysed human leukemic cells, HL-60 and K562. When Mφ were pretreated with IFN-γ and LPS simultaneously, they lysed much more leukemic cells. Furthermore, activated Mφ could induce lysis of leukemic cells separated from Mφ by a microporous membrane. As to the mechanism of leukemic cell lysis by activated Mφ, TNF was found to be at least an important effector molecule. It was also suggested that TNF and other labile factor(s) might be involved in the leukemic cell lysis. Next, we measured the concentration of various cytokines in the blood of leukemia patients before chemotherapy, at nadir, and on recovery phase. Among them, the concentration of IL-6 was high on recovery phase. G-CSF was high at early recovery phase in the blood of some patients. In the patients with high grade fever the concentration of G-CSF, M-CSF, and IFN-γ was high. Elevated concentration of IL-6 may contribute for early recovery of normal blood cells.Monocyte-derived Mφ of patients with leukemia, obtained during monocytosis, lysed HL-60 to a similar extent as control Mφ. The finding that activated human Mφ could lyse human leukemic cells suggests a role of Mφ in the eradication of leukemic cells in vivo and supports the possibility of clinical application of activated Mφ.","PeriodicalId":237003,"journal":{"name":"Journal of the Japan Society of the Reticuloendothelial System","volume":"5 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"126593200","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A case of adult-type Gaucher's disease associated with a right tibial tumor and hyperlipemia: its histological, histochemical, immunological, biochemical, enzymological and ultrastructural observations.","authors":"M. Naito","doi":"10.3960/JSLRT1961.23.151","DOIUrl":"https://doi.org/10.3960/JSLRT1961.23.151","url":null,"abstract":"","PeriodicalId":237003,"journal":{"name":"Journal of the Japan Society of the Reticuloendothelial System","volume":"348 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"122754028","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Leukemic malignant histiocytosis.","authors":"M. Motoi, S. Moriwaki, H. Toki, K. Okabe, T. Yoshino, T. Akagi","doi":"10.3960/JSLRT1961.31.43","DOIUrl":"https://doi.org/10.3960/JSLRT1961.31.43","url":null,"abstract":"Clinical, morphological, and ultrastructual studies are presented of three cases which had been diagnosed from lymph node biopsy as malignant histiocytosis. Three patients had a diffuse type of malignant histiocytosis and developed a leukemic phase at admission. Three patients survived for 1, 4 and 1 months after diagnosis. The neoplastic cells are characterized morphologically in the lymph nodes and in the peripheral blood by light microscopy and in two cases by electron microscopy also. These appear to be a spectrum of cell types in the leukemic malignant histiocytosis ranging from primitive reticulum cells or histiocytes to more differentiated monocytoid cells. No evidence for differentiation toward granulocytes was seen morphologically or immunohistochemically. The leukemic phase of malignant histiocytosis appears to be closely related to or identical histiocytic or monocytic leukemia (Schilling type) and should be differentiated from hairy cell leukemia. Necropsy findings from three cases are shortly reviewed.","PeriodicalId":237003,"journal":{"name":"Journal of the Japan Society of the Reticuloendothelial System","volume":"107 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"121815626","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}