Veterinary and comparative oncology最新文献

{"title":"Postmortem Detection of Blood Vascular Tumour-Cell Emboli in 31 Dogs: Macroscopic and Histological Findings.","authors":"Helenize Molozzi, Vanessa Cirinéa Tapia Barraza, Valentina Berté Marcus, Isabela de Aro Jorge Tavares, Maria Vitória Girol Sanches, Beatriz Regina Britz Zimmer, Bruna Karine Britz Zimmer, Flavia Alassia Sangoi, Rafael Almeida Fighera, Glaucia Denise Kommers, Mariana Martins Flores","doi":"10.1111/vco.70059","DOIUrl":"10.1111/vco.70059","url":null,"abstract":"<p><p>Despite the high prevalence of neoplastic diseases in dogs, tumour-cell embolism is poorly documented. This study aimed to characterise the presence and pathological features of distant blood vascular tumour-cell emboli (TCE) in dogs submitted for necropsy. TCE were histologically confirmed in 31/528 dogs (5.9%) that died or were euthanized due to neoplastic disease, most frequently mammary carcinomas (17/31, 54.8%). In 15/31 dogs (48.4%), TCE were associated with secondary macroscopic lesions, such as haemorrhage, whereas in 16/31 dogs (51.6%), there was no clear association with additional pathological changes. TCE were most commonly observed in the lungs (25/31), kidneys (9/31), adrenals (5/31), brain (5/31), and myocardium (5/31). Macroscopic pulmonary changes included petechiae or suffusions (8/31), and TCE-associated haemorrhages were also observed in the myocardium (4/31), kidneys (2/31), adrenals (2/31), brain (2/31), and gastrointestinal serosae (2/31). Histological lesions associated with TCE included haemorrhage (18/31), thrombosis (11/31), oedema (8/31), infarcts (7/31), and intimal proliferation and perivascular fibrosis (4/31). Taken together, these findings suggest that blood vascular TCE may be more prevalent in dogs than currently recognised and reinforce the importance of careful pathological evaluation for their detection. The clinical, macroscopic, and microscopic features described herein provide a practical framework for recognising TCE in veterinary pathology and underscore the need for further studies addressing their pathogenesis, clinical significance, and impact on disease progression in dogs.</p>","PeriodicalId":23693,"journal":{"name":"Veterinary and comparative oncology","volume":" ","pages":"353-362"},"PeriodicalIF":1.8,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147370435","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Use of Cardiac-Gated Computed Tomography for Assessment of 12 Dogs With Suspected Right Auricular Appendage Masses: A Case Series.","authors":"Josephine J Gendler, Eric T Hostnik, Hunter J Piegols, Janis M Lapsley, Laura E Selmic","doi":"10.1111/vco.70042","DOIUrl":"10.1111/vco.70042","url":null,"abstract":"<p><p>The cardiac-gated computed tomography (CT) scan reports of 12 dogs with right auricular appendage masses between 2018 and 2023 were reviewed to describe the use of cardiac-gated CT for assessment of right auricular appendage tumour location and extent and for staging. Imaging reports from thoracic radiographs, CT scans, abdominal ultrasound, and echocardiogram were evaluated for detection of a cardiac mass, mass location on the heart, and detection and location of suspected metastases. Surgery and necropsy reports were evaluated for definitive location of the cardiac mass. Descriptive statistics were performed. Cardiac-gated CT identified a mass that was confined to the right auricular appendage in seven dogs and involved additional cardiac structures in five dogs. Metastases were suspected on CT in 10 dogs. Surgery with the intent to remove the mass for cytoreduction was pursued in five dogs. Right auriculectomy was performed in four dogs. One dog whose mass was suspected to extend beyond the right auricular appendage on CT died during attempted surgical resection. Cardiac-gated CT helped guide clinical decision making by providing information about the location and extent of the mass to assess feasibility of surgical resection and also functioned as a sensitive staging test.</p>","PeriodicalId":23693,"journal":{"name":"Veterinary and comparative oncology","volume":" ","pages":"240-247"},"PeriodicalIF":1.9,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145901111","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Canine Urothelial DNA Mutations Are Not Associated With Total Trihalomethanes Concentrations in Municipal Drinking Water.","authors":"Janice O'Brien, Samantha L Braman, Jennifer Willcox, Phillip Wang, Daniel Gerrity, Audrey Ruple, Lauren A Trepanier","doi":"10.1111/vco.70060","DOIUrl":"10.1111/vco.70060","url":null,"abstract":"<p><p>Contaminants in municipal drinking water, specifically total trihalomethanes (TTHMs), are mutagenic. Exposures to TTHMs have been associated with urothelial carcinoma (UC) in both people and dogs. Mutations in the BRAF gene and other genomic copy number aberrations detected in voided urothelial cells with commercial CADET BRAF and BRAF-PLUS tests have also been associated with the presence of UC in dogs. However, it is not known whether these urothelial mutations can be linked to TTHM exposures in dogs. The objectives of this ecological study were to compare the incidence of detected urinary BRAF mutations or genomic copy number aberrations in dogs residing in a city with relatively high drinking water TTHMs (Las Vegas, NV) to a city with significantly lower TTHMs (Reno, NV), and to expand the study group to include BRAF and BRAF-PLUS test results and municipal drinking water TTHM concentrations by zip code across the United States. Dogs living in Las Vegas had a higher relative risk of urothelial mutations compared to dogs living in Reno (RR 2.52; 95% CI, 1.77-3.38; p < 0.0001). However, this risk could not be attributed to higher municipal drinking water TTHMs. Across the United States, the population-adjusted incidence of urothelial mutations in voided urine was not associated with municipal water TTHMs, but was instead associated in cross-sectional analyses with age, neutered status, higher regional test submission rates and previously reported high-risk breeds for UC (Scottish terriers, West Highland white terriers, Shetland sheepdogs, beagles and wirehaired fox terriers). The higher RR for a positive BRAF or BRAF-PLUS test in Las Vegas versus Reno could be solely due to higher test submission rates (2.97 versus 0.97 per 1000 dogs) but could also reflect other environmental exposures not considered in this study.</p>","PeriodicalId":23693,"journal":{"name":"Veterinary and comparative oncology","volume":" ","pages":"363-369"},"PeriodicalIF":1.8,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13161735/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147435986","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Prognostic Value of Magnetic Resonance Imaging Defined Extent of Surgical Resection in Dogs With Intracranial Meningiomas.","authors":"John H Rossmeisl, Rell L Parker, Richard L Shinn, Josefa K Garcia Mora, Thomas E Cecere, Teresa Southard, John L Robertson","doi":"10.1111/vco.70044","DOIUrl":"10.1111/vco.70044","url":null,"abstract":"<p><p>Surgery is a common treatment for intracranial meningiomas in dogs, although the prognostic impact of the extent of resection (EOR) has not been systematically evaluated. This retrospective study identified prognostic factors associated with clinical outcomes in dogs that underwent surgery and early post-operative magnetic resonance imaging (epoMRI) to evaluate meningioma EOR. We hypothesised that gross total tumour resection (GTR) would result in longer progression free (PFS) and overall survival (OS), and superior post-operative seizure control and resolution of neurological dysfunction than subtotal resection (STR). Multivariable logistic regression was used to identify prognostic factors, and Kaplan-Meier analyses to compare survival outcomes. Forty-one dogs were included of which 24 (59%) had GTR and 17 (41%) had STR. GTR was associated with decreased rates of tumour progression (HR = 0.21; 95% CI, 0.09-0.42; p < 0.0001) and death (HR = 0.49; 95% CI, 0.14-0.69; p < 0.0001), and longer PFS (618 vs. 189 days, p < 0.0001) and OS (694 vs. 349 days, p < 0.0001) compared to STR. Higher tumour grade and increasing age negatively impacted PFS and OS, respectively. Seizure freedom was attained in a larger proportion of dogs with GTR (18/20 [90%]) than STR (4/13 [31%]; p < 0.001), but rates of improvement of neurological deficits were not different between groups. GTR resulted in durable clinical improvements and survivals in the absence of adjuvant treatments. EpoMRI to assess EOR should be routinely incorporated into management of canine meningiomas to inform outcome expectations, and to identify STR cases in which adjuvant therapies should be considered.</p>","PeriodicalId":23693,"journal":{"name":"Veterinary and comparative oncology","volume":" ","pages":"260-273"},"PeriodicalIF":1.8,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13161732/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145990921","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Multi-Institutional Retrospective Study of 21 Dogs Having Undergone Hypofractionated Radiotherapy for Adrenal Tumours (2017-2024).","authors":"Yen-Hao Erik Lai, Michael S Kent, Michael W Nolan, Dah-Renn Fu, Jayme S Looper","doi":"10.1111/vco.70041","DOIUrl":"10.1111/vco.70041","url":null,"abstract":"<p><p>Radiation therapy (RT) has emerged as a promising non-surgical approach for treating canine adrenal tumours. This multi-institutional, retrospective study describes clinical outcomes for 21 dogs having been prescribed a course of hypofractionated image-guided intensity-modulated RT (IMRT) entailing delivery of 25-35 Gy total in 5 fractions given over 5-15 days for an adrenal tumour. Diagnoses were based on imaging (abdominal ultrasound or computed tomography) and biochemical testing. All dogs had unilateral or bilateral irregular adrenal masses with evidence of vessel compression or invasion. Adrenal masses were incidentally identified in 11 dogs. The clinical diagnoses included pheochromocytoma (n = 13, 61.9%), adrenocortical adenocarcinoma (n = 2, 9.5%) and unspecified (n = 6, 28.6%). Among the 16 dogs with available follow-up imaging, the rates of partial response and stable disease were 37.5% (6/16) and 62.5% (10/16), respectively. Mild gastrointestinal side effects related to RT were reported in four dogs (19%). Early death that could have been attributable to tumour or complications of treatment occurred in two dogs (9.5%) at 21 and 52 days post-RT; one presenting with acute vomiting, and the other presenting with vomiting, tremors, and shock before death. Of the 15 deceased dogs, 4 (26.7%) died due to tumour-related causes and 11 died due to unknown (n = 2) or unrelated (n = 9) causes. The median overall survival time was 377 days, with a median follow-up time of 458 days for censored patients (n = 6). The one- and two-year survival rates were 59.4% and 34.7%, respectively. These data build upon prior published reports, demonstrating that RT can be associated with prolonged survival in dogs with adrenal tumours. Hypofractionated IMRT appears to offer a potential survival benefit even in dogs with major vessel invasion or comorbidities. Future research should focus on identifying risk factors for early death and determining which patient populations are most likely to benefit from RT.</p>","PeriodicalId":23693,"journal":{"name":"Veterinary and comparative oncology","volume":" ","pages":"229-239"},"PeriodicalIF":1.8,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13161733/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145906938","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Whole Exome Sequencing of Feline Oral Squamous Cell Carcinoma Reveals Genomic Parallels With Human Head and Neck Squamous Cell Carcinoma.","authors":"Shirley Chu, Zachary L Skidmore, Wes Warren, Bryan Fisk, Dae Young Kim, Santiago Peralta, Alana Rodney, Jennifer Bryant, Catrina Fronick, Owen Skinner, Cindy Bell, Amanda Gruber, Lara Bartl-Wilson, Scott Neiberg, Gwen Bilyk, Janet Coffman, Kristen Wischmann, Tami Beckemen, Christy Fischer, Alec Flaum, William Crouch, Denise Montrose, Kenton Hoernig, Malachi Griffith, Jeffrey N Bryan, Obi L Griffith","doi":"10.1111/vco.70054","DOIUrl":"10.1111/vco.70054","url":null,"abstract":"<p><p>Feline oral squamous cell carcinoma (FOSCC) and human head and neck squamous cell carcinoma (HNSCC) are the 4th most common malignant neoplasms in cats and humans. Most cats are not treated due to the poor prognosis and die of their disease within a few months of diagnosis. In humans, despite surgery, radiation therapy, and chemotherapy, 1/3 of patients develop loco-regional recurrence within 5 years. FOSCC has commonly been proposed as a model for HNSCC since FOSCC is a spontaneous model and exhibits similar biologic behaviour, treatment course, and outcome to the human disease. The objective of this study was to identify genetic similarities and differences with HNSCC, therapeutic targets, and possible causes of FOSCC through whole exome sequencing. Thirty-six matched normal FOSCC tumours and three matched normal chronic gingivostomatitis lesions were sequenced with a previously validated custom Roche Nimblegen exome reagent, based on the Felis_catus_9.0 genome assembly, covering 35.7 Mb. The exome libraries were pooled and sequenced on the Illumina Novaseq6000 as 2 × 150 bp reads. The data was analysed with the McDonnell Genome Institute's (MGI) cancer informatics pipelines, namely the Genome Modelling System (GMS), which was adapted to the feline genome. TP53 harboured consequential variants in 71% of the FOSCC tumours and was the most commonly mutated gene. Consequential variants in TTN were seen in 18% tumours and consequential variants in FSIP2, LRP1B, RYR2 were seen 9% of tumours. Genes with highly recurrent copy number variants (CNVs) included CKDN2A, PTEN, and MYC. TMB was very low, 0-1.6 (mean 0.66). Six cats had a history of chronic gingivostomatitis for years before the diagnosis of FOSCC. Despite the low TMB, the genomic landscape of FOSCC parallels HPV-negative HNSCC. Aside from TP53, recurrent small-scale mutations in FOSCC were uncommon, but recurrent CNVs were common. FOSCC is a genetically and clinically heterogenous cancer. A multifactorial cause is suspected. Chronic gingivostomatitis, immunosuppression, and/or viral infection may be associated with FOSCC in young cats.</p>","PeriodicalId":23693,"journal":{"name":"Veterinary and comparative oncology","volume":" ","pages":"300-312"},"PeriodicalIF":1.8,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13420018/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147271985","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Recurrence Rates for Mandibular Fibrosarcoma Associated With Mandibular Canal Invasion Following Mandibulectomy Procedures in Dogs.","authors":"Carley P Johnson, Josephine A Dornbusch, Owen T Skinner, Janet A Grimes, Julius M Liptak, Megan T Cray, Michelle Oblak, William T N Culp, Marine Traverson, Vincent A Wavreille, Hunter J Piegols, Laura E Selmic","doi":"10.1111/vco.70064","DOIUrl":"10.1111/vco.70064","url":null,"abstract":"<p><p>Oral fibrosarcoma is a highly invasive tumour with local recurrence rates of up to 57.9% following surgical excision. The optimal surgical margins for long-term local control of oral fibrosarcoma have not been ascertained, especially when tumour invasion into the mandibular canal is present. The objective of this study was to evaluate the recurrence of mandibular fibrosarcoma in dogs following mandibulectomy procedures based on the presence or absence of mandibular canal invasion. Medical records of dogs that underwent a mandibulectomy for fibrosarcoma between January 2005 and December 2023 at 9 veterinary referral centers were reviewed. The size of the tumour, surgical procedure performed, surgical margins obtained, and long-term outcome were recorded. Fisher's exact and Mann-Whitney U tests were used to compare the association between different factors with development of local recurrence. Forty-nine dogs were included. Surgeries performed included segmental mandibulectomy (n = 14), unilateral mandibulectomy (n = 11), bilateral rostral mandibulectomy (n = 9), unilateral rostral mandibulectomy (n = 5), and three-quarter (¾) mandibulectomy (n = 3). Forty dogs had complete histologic margins. Mandibular canal invasion was reported in 14 dogs. Follow-up was available for a median of 493 days postoperatively. Eight dogs were diagnosed with local recurrence. Five dogs with local recurrence had mandibular canal invasion at the initial surgery. No variables evaluated individually were significantly associated with local recurrence. Multivariable logistic regression found the absence of mandibular canal invasion was associated with reduced odds of recurrence (odds ratio, 0.06; 95% confidence interval 0.004-0.835; p = 0.036) when adjusted for the type of mandibulectomy performed and the histopathologic margin status. Further research is needed to determine the optimal surgical margins for dogs with mandibular fibrosarcoma.</p>","PeriodicalId":23693,"journal":{"name":"Veterinary and comparative oncology","volume":" ","pages":"407-415"},"PeriodicalIF":1.8,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13161737/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147595019","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Clinical Efficacy and Tolerability of Sorafenib in Dogs With Advanced Carcinomas.","authors":"Doyun Kim, Taekun Kim, Youngsun Kim, Hojin Jang, Hanbin Lee, Youngmin Yun, Mihyun Choi, Woo-Jin Song","doi":"10.1111/vco.70047","DOIUrl":"10.1111/vco.70047","url":null,"abstract":"<p><p>Advanced carcinomas in dogs present significant therapeutic challenges with limited treatment options and poor prognoses. Sorafenib, a multi-kinase inhibitor targeting RAF and receptor tyrosine kinases, has demonstrated antitumor activity and tolerability in preclinical veterinary studies; however, clinical data remain limited. This prospective, open-label cohort study evaluated the tolerability and clinical efficacy of oral sorafenib (6 mg/kg once daily) in 24 dogs with cytologically or histologically confirmed unresectable, recurrent, or metastatic carcinomas. Tumour response was assessed using RECIST criteria, and adverse events were graded according to VCOG-CTCAE v2.0. Stable disease at 1 month was observed in 14 of 18 evaluable dogs (77.8%), yielding an overall clinical benefit rate of 58.3%. Median time to progression and overall survival were 51 days (95% CI, 14-115) and 65 days (95% CI, 49-143), respectively. Dogs presenting with lameness (n = 7) showed significantly longer time to progression and overall survival than those without (p < 0.01). Adverse events occurred in 9 of 24 dogs (37.5%), primarily mild lameness (29%) and gastrointestinal signs. Weight gain at 1 month, observed in 66.7% of dogs, was significantly associated with clinical benefit (p = 0.005; OR = 45.0; 95% CI, 1.8-1127.7). Sorafenib was well tolerated and provided measurable clinical benefit in dogs with advanced carcinomas. Lameness may represent a prognostic biomarker, and weight gain may reflect a favourable clinical response. These findings support further investigation of sorafenib and other tyrosine kinase inhibitors in veterinary oncology.</p>","PeriodicalId":23693,"journal":{"name":"Veterinary and comparative oncology","volume":" ","pages":"274-283"},"PeriodicalIF":1.8,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146087396","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genomic Landscape Analysis of Canine Pulmonary Adenocarcinoma Reveals Candidate Targetable Gene Fusions.","authors":"Sharadha Sakthikumar, William P D Hendricks, David Rainford, William Selleck, Natalia Briones, Christopher Coggins, Natalie Quan, Victoria Zismann, Gwendolen Lorch, Aleksandar Sekulic, Jeffrey M Trent","doi":"10.1111/vco.70063","DOIUrl":"10.1111/vco.70063","url":null,"abstract":"<p><p>Spontaneously occurring primary canine pulmonary adenocarcinoma (cPAC) exhibits clinicopathological and molecular similarities to never-smoker human lung cancers. Shared genomic alterations, including point mutations, indel mutations and copy number changes particularly in HER2 signalling, are significant therapeutic targets, especially for HER2 and tyrosine kinase inhibitors. Whilst progress has been made in identifying mutational drivers in canine cancers, the role of somatic gene fusions in cPAC remains poorly understood, despite their importance in other cancers as drivers and therapeutic targets. This study investigates the fusion landscape in cPAC by analysing RNA-seq data from a cohort of 36 primary tumour samples and reports oncogenic fusions with therapeutic potential. Notably, NRG1 fusions were identified in a subset of tumours, including recurrent SDC4::NRG1 events, potentially playing key roles in disease progression. NRG1 fusions, known to activate HER2 signalling, are mutually exclusive with HER2 gene alterations, indicating convergence on the same pathway. Tumours with SDC4::NRG1 fusions also overexpress HER2 pathway-related genes, reinforcing NRG1-driven activation. Similar fusions occur in never-smoker human non-small cell adenocarcinoma lacking other common drivers, underscoring their therapeutic importance. These findings highlight NRG1 fusions as critical contributors to cPAC tumorigenesis and warrant further clinical and comparative investigation. Additionally, novel fusions disrupting the PTEN axis were identified, leading to truncated PTEN and associated protein domains. These disruptions could impair tumour-suppressive pathways, presenting additional therapeutic targets. This research emphasises the broader relevance of fusion-driven mechanisms in cPAC tumorigenesis, advancing the understanding of both canine and human lung cancers for clinical and comparative studies.</p>","PeriodicalId":23693,"journal":{"name":"Veterinary and comparative oncology","volume":" ","pages":"393-406"},"PeriodicalIF":1.8,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13161739/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147582414","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Generation of a Rabbit Anti-Canine CD19 Monoclonal Antibody From Peripheral Blood and Its Validation in Immunoassays and CAR-T Feasibility.","authors":"Huixin Li, Qiqi Lu, Xin Huang, Mengyan Li, Yiting Zhou, Mengjuan Chen, Mengke Qin, Jingjing Kang, Shanshan Xie, Qingda Meng","doi":"10.1111/vco.70061","DOIUrl":"10.1111/vco.70061","url":null,"abstract":"<p><p>Lymphoma constitutes 24% of canine neoplastic diseases and 85% of haematopoietic tumours, with B-cell subtypes accounting for 60%-80% of cases. As the most prevalent spontaneous tumour in canines, this disease model holds significant translational value for human non-Hodgkin lymphoma research. To address diagnostic limitations in canine B-cell lymphoma, we developed a canine-specific CD19 monoclonal antibody (HAC19.1) with high affinity and established a dual-platform detection system compatible with flow cytometry and immunohistochemistry. Additionally, a novel CD19-targeting chimeric antigen receptor (CAR) gene sequence (HUA-1) was engineered and successfully transduced into Jurkat cells via lentiviral vectors, confirming stable CAR membrane expression. This breakthrough provides critical technical groundwork for advancing autologous CAR-T cell therapy in canines.</p>","PeriodicalId":23693,"journal":{"name":"Veterinary and comparative oncology","volume":" ","pages":"370-381"},"PeriodicalIF":1.8,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147475759","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}