将循环游离细胞 DNA 作为液体活检对患有各种肿瘤的猫的临床实用性

IF 2.3 2区 农林科学 Q1 VETERINARY SCIENCES
Veterinary and comparative oncology Pub Date : 2024-12-01 Epub Date: 2024-10-09 DOI:10.1111/vco.13013
Michihito Tagawa, Hotaka Hiroi, Yuzuki Nakano, Riyo Morishita, Kosuke Kobayashi, Osamu Sakai
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引用次数: 0

摘要

目前,兽医领域(尤其是猫科动物)只有数量有限的肿瘤生物标记物可用。无细胞DNA(cfDNA)是细胞死亡时释放的细胞外DNA片段,被认为是诊断和监测各种人类恶性肿瘤的微创生物标记物。本研究旨在阐明循环 cfDNA 作为各种猫科动物肿瘤的液体活检的实用性。研究人员收集了 44 只患有各种肿瘤的猫咪、24 只患有其他疾病的猫咪和 10 只健康对照组猫咪的血浆样本。对 3 名肿瘤患者进行了随访研究。所有 cfDNA 浓度都通过实时聚合酶链式反应(PCR)进行了量化,PCR 提供了新发现的猫科 LINE-1 基因的长短片段。我们发现,患有各种肿瘤的猫的 cfDNA 水平明显高于患有其他疾病的猫或健康对照组。cfDNA浓度与血清淀粉样蛋白A(SAA)水平无关。患有肿瘤的猫的 cfDNA 水平升高,预测其患有肿瘤的灵敏度和特异度分别为 50.5%和 91.2%(AUC 0.736;P<0.05)。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Clinical Utility of Circulating Cell-Free DNA as a Liquid Biopsy in Cats With Various Tumours.

Only a limited number of tumour biomarkers are currently available in veterinary medicine, particularly in cats. Cell-free DNA (cfDNA) is an extracellular DNA fragment released upon cell death and is considered a minimally invasive biomarker for the diagnosis and monitoring of various human malignancies. This study aimed to clarify the utility of circulating cfDNA as a liquid biopsy for various feline tumours. Plasma samples were collected from 44 cats with various tumours, 24 cats with other diseases and 10 healthy controls. A follow-up study was conducted in three tumour-bearing patients. All cfDNA concentrations were quantified via real-time polymerase chain reaction (PCR), which provided short and long fragments of a newly identified feline LINE-1 gene. We found that cfDNA levels were significantly higher in cats with various tumours than in those with other diseases or healthy controls. The cfDNA concentration was not correlated with serum amyloid A (SAA) levels. Cats with tumours exhibited elevated cfDNA levels that predicted tumour-bearing with a sensitivity and specificity of 50.5% and 91.2%, respectively (AUC 0.736; p < 0.001). In lymphoma cases, cats with high cfDNA levels had significantly shorter survival times than those with low cfDNA levels (median: 33 days vs. 178 days; p = 0.003). In addition, the cfDNA levels of the three patients correlated with clinical status during follow-up. Collectively, these findings indicate the potential of cfDNA as a useful biomarker for the diagnosis, therapeutic monitoring and prognostic assessment of tumours in cats.

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来源期刊
Veterinary and comparative oncology
Veterinary and comparative oncology 农林科学-兽医学
CiteScore
4.80
自引率
9.50%
发文量
75
审稿时长
>24 weeks
期刊介绍: Veterinary and Comparative Oncology (VCO) is an international, peer-reviewed journal integrating clinical and scientific information from a variety of related disciplines and from worldwide sources for all veterinary oncologists and cancer researchers concerned with aetiology, diagnosis and clinical course of cancer in domestic animals and its prevention. With the ultimate aim of diminishing suffering from cancer, the journal supports the transfer of knowledge in all aspects of veterinary oncology, from the application of new laboratory technology to cancer prevention, early detection, diagnosis and therapy. In addition to original articles, the journal publishes solicited editorials, review articles, commentary, correspondence and abstracts from the published literature. Accordingly, studies describing laboratory work performed exclusively in purpose-bred domestic animals (e.g. dogs, cats, horses) will not be considered.
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