Veterinary and comparative oncology最新文献

Classification and Prognostication of B-Cell and T-Cell Multicentric Lymphoma in Dogs Using Serum MicroRNAs.

IF 2.3 2区农林科学

Veterinary and comparative oncology Pub Date : 2025-04-05 DOI: 10.1111/vco.13057

Latasha Ludwig, Heather Treleaven, Roger Moorehead, Robert A Foster, R Darren Wood, R Ayesha Ali, Geoffrey A Wood

{"title":"Classification and Prognostication of B-Cell and T-Cell Multicentric Lymphoma in Dogs Using Serum MicroRNAs.","authors":"Latasha Ludwig, Heather Treleaven, Roger Moorehead, Robert A Foster, R Darren Wood, R Ayesha Ali, Geoffrey A Wood","doi":"10.1111/vco.13057","DOIUrl":"https://doi.org/10.1111/vco.13057","url":null,"abstract":"Canine multicentric lymphoma is a common malignancy in dogs. It often responds well to initial chemotherapy but frequently relapses and has a poor response to subsequent treatment. B-cell (BCL) and T-cell (TCL) lymphomas differ in both their prognoses and chemotherapeutic treatment protocols. Currently, immunophenotyping can be costly and can only be performed on specific high-quality samples. MicroRNAs (miRNAs) are small molecules present in blood and tissues and are dysregulated in both human and canine lymphoma. We investigated 59 miRNAs by RT-qPCR to establish a serum miRNA profile in dogs with B-cell and T-cell multicentric lymphoma. Multiple miRNA pruned decision tree models were used to classify BCL and TCL cases from each other and controls, and to predict prognosis in BCL cases receiving standard CHOP chemotherapy. Six individual miRNAs were differentially expressed in serum between BCL and controls, and three were differentially expressed between BCL and TCL. A three-miRNA model (miR-155-5p, miR-1 and miR-181b) could differentiate between BCL, TCL and control samples with an accuracy of 83.02%. A three-miRNA model (miR-125b-5p, miR-350 and let-7b-5p) in BCL samples separated the cases into four groups with hazard ratios ranging from 0.44 to 3.5 for overall survival. This study established a serum miRNA profile for both BCL and TCL and demonstrated the utility of multiple serum miRNA models to assist in the diagnosis of lymphoma and BCL prognostication.","PeriodicalId":23693,"journal":{"name":"Veterinary and comparative oncology","volume":" ","pages":""},"PeriodicalIF":2.3,"publicationDate":"2025-04-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143789116","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Poly (ADP-Ribose) Polymerase-1 Enzyme Is Overexpressed in Various Solid Canine Tumours: An Immunohistochemical Study.

IF 2.3 2区农林科学

Veterinary and comparative oncology Pub Date : 2025-04-03 DOI: 10.1111/vco.13053

Shana De Vos, Simone Janssen, Sophie Favril, Hilde De Cock, Matthijs Vynck, Jonas Steenbrugge, Ward De Spiegelaere, Hilde de Rooster

{"title":"The Poly (ADP-Ribose) Polymerase-1 Enzyme Is Overexpressed in Various Solid Canine Tumours: An Immunohistochemical Study.","authors":"Shana De Vos, Simone Janssen, Sophie Favril, Hilde De Cock, Matthijs Vynck, Jonas Steenbrugge, Ward De Spiegelaere, Hilde de Rooster","doi":"10.1111/vco.13053","DOIUrl":"https://doi.org/10.1111/vco.13053","url":null,"abstract":"The enzyme poly (ADP-ribose) polymerase-1 (PARP-1) is crucial for cellular regulation and DNA repair. Its immunohistochemical overexpression is known in various human neoplasms, but it was not yet a topic of veterinary research. Formalin-fixed paraffin-embedded canine samples of 15 controls and 34 tumours were immunohistochemically tested for PARP-1 expression. Controls included five skin samples with mast cells, five oral mucosa samples and five thyroid glands. Tumours included 18 mast cell tumours (MCTs), 10 oral squamous cell carcinomas (SCCs) and six follicular thyroid carcinomas. A board-certified veterinary pathologist defined the optimal region for the blind PARP-1 evaluation, assessed by two independent veterinary PhD students. Positive nuclei were evaluated by the immunoreactivity score (IRS) and quick score (QS) and, for both scores, the averages of the two observers were used for statistical analysis. In all MCTs, 6/10 SCCs and all thyroid carcinomas as well as four thyroid controls a nuclear expression was observed. A cytoplasmic granular staining was visible in all dermal mast cells and in 11/18 MCTs due to non-specific antibody uptake. No PARP-1 was expressed in 11/15 controls.Compared to the controls, thyroid carcinomas significantly overexpressed PARP-1 when calculated by IRS and QS (p = 0.003 and p = 0.005, respectively). The latter also applied to the MCTs (p = 0.001). A significantly higher PARP-1 IRS and QS were observed in thyroid carcinomas (p = 0.003, p = 0.005) and MCTs (p = 0.003, p = 0.012) compared to oral SCCs. The immunohistochemical PARP-1 overexpression in these tumours invites further research to assess its potential as a therapeutic target.","PeriodicalId":23693,"journal":{"name":"Veterinary and comparative oncology","volume":" ","pages":""},"PeriodicalIF":2.3,"publicationDate":"2025-04-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143773127","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Chemoresistance Cytological Score for Canine Transmissible Venereal Tumour: A Proposed System of Predicting Vincristine Resistance.

IF 2.3 2区农林科学

Veterinary and comparative oncology Pub Date : 2025-03-30 DOI: 10.1111/vco.13054

Pedro Antônio Bronhara Pimentel, Mariana Pádua Costa, Isadora Maria Sátiro de Oliveira, Ayisa Rodrigues Oliveira, Paulo Ricardo Oliveira Paes, Ayla Watanabe Pereira, Camila Stefanie Fonseca de Oliveira, Rodrigo Dos Santos Horta

{"title":"Chemoresistance Cytological Score for Canine Transmissible Venereal Tumour: A Proposed System of Predicting Vincristine Resistance.","authors":"Pedro Antônio Bronhara Pimentel, Mariana Pádua Costa, Isadora Maria Sátiro de Oliveira, Ayisa Rodrigues Oliveira, Paulo Ricardo Oliveira Paes, Ayla Watanabe Pereira, Camila Stefanie Fonseca de Oliveira, Rodrigo Dos Santos Horta","doi":"10.1111/vco.13054","DOIUrl":"https://doi.org/10.1111/vco.13054","url":null,"abstract":"Canine transmissible venereal tumour (CTVT) is a common diagnosis in many low-income countries, and managing its chemoresistant cases can be challenging within the conventional vincristine sulfate chemotherapy protocol. Also, predictive markers of chemoresistance for this tumour remain unidentified. This study aimed to evaluate CTVT cytological characteristics to develop a predictive cytological scoring system for vincristine sulfate resistance. For this purpose, 40 cases were retrospectively analysed based on their clinical aspects and response to vincristine chemotherapy. First, cytological preparations underwent a double-blind assessment to evaluate a modified cytomorphological classification. This classification, an adaptation of a previously published system, categorised tumours based on cell shape (plasmacytic versus lymphocytic), but no association with chemoresistance was found (p = 0.083). Subsequently, a novel cytology scoring system was developed and tested to identify cases potentially associated with chemoresistance. This system was based on three criteria: anisokaryosis, mitotic count, and the presence of binucleated cells. Malignancy criteria, evaluated in 5 hotspots, were inversely associated with chemoresistance (p = 0.001), predicted by low anisokaryosis, a mitotic count of ≤ 6 in a 2.37 mm2 area, and no binucleated cells. This study introduces a novel cytology scoring system for CTVTs, designed to serve as a predictive tool for vincristine treatment response. This system has the potential to aid practitioners in clinical decision-making. However, further studies are required to validate its reliability and applicability.","PeriodicalId":23693,"journal":{"name":"Veterinary and comparative oncology","volume":" ","pages":""},"PeriodicalIF":2.3,"publicationDate":"2025-03-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143753832","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genotoxic Herbicide Exposures in Golden Retrievers With and Without Multicentric Lymphoma. 患有和未患有多中心淋巴瘤的金毛寻回犬暴露于遗传毒性除草剂的情况。

IF 2.3 2区农林科学

Veterinary and comparative oncology Pub Date : 2025-03-21 DOI: 10.1111/vco.13051

Ashleigh N Tindle, Lauren M Krueger, Brenna Swafford, Erin Mani, Camille Danielson, Julia Labadie, Lauren A Trepanier

{"title":"Genotoxic Herbicide Exposures in Golden Retrievers With and Without Multicentric Lymphoma.","authors":"Ashleigh N Tindle, Lauren M Krueger, Brenna Swafford, Erin Mani, Camille Danielson, Julia Labadie, Lauren A Trepanier","doi":"10.1111/vco.13051","DOIUrl":"https://doi.org/10.1111/vco.13051","url":null,"abstract":"Canine multicentric lymphoma (CML) is one of the most common malignancies in dogs. Although breed risk is important, environmental factors such as herbicides have also been implicated. The objective of this study was to determine whether genotoxic exposures to the herbicides 2,4-D and glyphosate are associated with CML, using dogs from the Golden Retriever Lifetime Study cohort. We measured urinary concentrations of glyphosate and 2,4-D in golden retrievers with CML and matched unaffected controls at two time points: at the time of diagnosis and 1 year prior to diagnosis. To assess the genotoxic potential of herbicide exposures, we used reverse dosimetry from urinary concentrations to estimate plasma concentrations. We then assessed the genotoxicity of these herbicide concentrations towards healthy canine peripheral blood mononuclear cells (PBMC's) in vitro using the CometChip assay, with and without canine liver microsomes. All dogs had detectable urinary exposures to 2,4-D (7.3-42.9 ng/mg creat) and glyphosate (0.4-80.7 ng/mg creat), with no differences between cases and controls at either time point. Both 2,4-D and glyphosate were genotoxic to canine PBMCs at concentrations of 0.10 μM and higher, with no consistent effects of canine liver microsomes on herbicide genotoxicity. No dogs reached estimated genotoxic plasma concentrations for glyphosate, but 4 of 30 golden retrievers with CML (13.3%) and 2 of 30 control dogs (6.7%) reached estimated genotoxic 2,4-D exposures (p = 0.67).","PeriodicalId":23693,"journal":{"name":"Veterinary and comparative oncology","volume":" ","pages":""},"PeriodicalIF":2.3,"publicationDate":"2025-03-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143671086","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Retrospective Study Evaluating the Outcome and Efficacy of Stereotactic Body Radiation Therapy for the Treatment of Metastatic Abdominal Lymph Nodes in Dogs With Apocrine Gland Anal Sac Adenocarcinoma.

IF 2.3 2区农林科学

Veterinary and comparative oncology Pub Date : 2025-03-20 DOI: 10.1111/vco.13052

Tiffany W Martin, Theodore Chang, Mary-Keara Boss, Brandan Janssens, Susan M LaRue

{"title":"Retrospective Study Evaluating the Outcome and Efficacy of Stereotactic Body Radiation Therapy for the Treatment of Metastatic Abdominal Lymph Nodes in Dogs With Apocrine Gland Anal Sac Adenocarcinoma.","authors":"Tiffany W Martin, Theodore Chang, Mary-Keara Boss, Brandan Janssens, Susan M LaRue","doi":"10.1111/vco.13052","DOIUrl":"https://doi.org/10.1111/vco.13052","url":null,"abstract":"Local treatment for dogs with regional lymph node metastasis secondary to apocrine gland anal sac adenocarcinoma (AGASACA) includes nodal extirpation or radiotherapy. Stereotactic body radiation therapy (SBRT) may provide a definitive intent treatment option for macroscopic nodal disease when surgery is declined or the disease is deemed inoperable. Twenty-five dogs receiving SBRT to the metastatic sacroiliac lymph nodes were retrospectively evaluated. Dogs were staged according to the previously published TNM staging system with 3 stage IIIa, 14 stage 3b, and 8 stage IV. The overall median survival time (MST) was 451 days and the stage did not significantly impact survival (p = 0.31). The overall median event-free survival time was 246 days. Significant positive prognostic factors included male sex, higher dose per fraction, and higher total dose (p = 0.034, 0.0035, 0.0047). Dogs receiving 6-7.5 Gy per fraction with a total dose of 30-37.5 Gy outperformed dogs receiving other protocols. Twelve dogs experienced gait changes in the hind limbs during the late radiation effects period. Resolution of hypercalcemia in 5 dogs was inconsistent and transient. The best response was complete in 21%, partial in 58%, and stable disease in 17% at a median of 100 days. Three dogs (12%) developed progression of treated lymph nodes at 157, 498, and 644 day. Eight dogs (32%) had recurrence of their primary (untreated by radiation) anal sac masses. SBRT was determined to be an effective alternative to surgical excision; however, more investigation is needed to determine the cause of gait changes in the late toxicity period.","PeriodicalId":23693,"journal":{"name":"Veterinary and comparative oncology","volume":" ","pages":""},"PeriodicalIF":2.3,"publicationDate":"2025-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143664585","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Characterisation of Gene Expression in Canine Invasive Urothelial Carcinoma Using a NanoString-Based Urine Assay.

IF 2.3 2区农林科学

Veterinary and comparative oncology Pub Date : 2025-03-19 DOI: 10.1111/vco.13046

Ellen Marie Kuerbitz, Deepika Dhawan, Sagar Utturkar, Mario Francis Sola, Alexander William Enstrom, Lindsey Marie Fourez, Deborah Wagner Knapp

{"title":"Characterisation of Gene Expression in Canine Invasive Urothelial Carcinoma Using a NanoString-Based Urine Assay.","authors":"Ellen Marie Kuerbitz, Deepika Dhawan, Sagar Utturkar, Mario Francis Sola, Alexander William Enstrom, Lindsey Marie Fourez, Deborah Wagner Knapp","doi":"10.1111/vco.13046","DOIUrl":"https://doi.org/10.1111/vco.13046","url":null,"abstract":"For human and canine invasive urothelial carcinoma (InvUC), there is growing interest in using the molecular characteristics of a tumour to guide individualised treatment strategies. The objective of this study was to use a non-invasive, urine-based method to characterise gene expression signatures in dogs with InvUC. RNA was isolated from canine InvUC tumour samples, urine sediment from dogs with InvUC, normal canine bladder mucosa, and normal canine urine sediment and queried using the nCounter Canine Immuno-Oncology Panel. Differential gene expression profiles were characterised for tissue and urine samples, and nCounter results were compared to bulk RNA-seq gene expression profiles. The effect of spiking normal urine with white blood cells (WBCs) from the same dog was also assessed. Key genes involved in antitumor immune responses and oncogenic signalling pathways, including potential small molecule inhibitor targets, were differentially expressed in tumour and urine samples from dogs with InvUC, compared to normal samples. nCounter-generated gene expression profiles for tumour tissue and urine from dogs with InvUC were highly correlated, whereas the correlation between the nCounter IO panel and bulk RNA-seq results for InvUC tissue was moderate. The addition of WBCs to normal urine affected the gene expression profiles. Analysis of canine urine using the nCounter canine IO panel has good potential for revealing gene expression patterns in InvUC. Additional studies are warranted to determine the extent to which WBC infiltration affects the results related to immune response patterns and the expression of other genes.","PeriodicalId":23693,"journal":{"name":"Veterinary and comparative oncology","volume":" ","pages":""},"PeriodicalIF":2.3,"publicationDate":"2025-03-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143658880","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Half-Body Radiation Therapy Results in a Prolonged Progression-Free Interval in Canine High-Grade Lymphoma After First Remission.

IF 2.3 2区农林科学

Veterinary and comparative oncology Pub Date : 2025-03-15 DOI: 10.1111/vco.13050

Yen-Hao Erik Lai, Sarah Lyles, Mark Mitchell, Jayme Looper

{"title":"Half-Body Radiation Therapy Results in a Prolonged Progression-Free Interval in Canine High-Grade Lymphoma After First Remission.","authors":"Yen-Hao Erik Lai, Sarah Lyles, Mark Mitchell, Jayme Looper","doi":"10.1111/vco.13050","DOIUrl":"https://doi.org/10.1111/vco.13050","url":null,"abstract":"An optimal protocol of adding wide-field irradiation to multi-agent chemotherapy for dogs with lymphoma has not been established. The aim of this retrospective case-control study was to evaluate the efficacy of a protocol combining chemotherapy and half-body irradiation (HBI) for dogs with high-grade lymphoma. Dogs in the treatment group received cranial HBI 2 weeks after completing the second cycle of the multi-agent chemotherapy protocol. The radiation therapy protocol consisted of 4 Gy/fraction once per day for 2 consecutive days for the cranial half body, followed by the same protocol for the caudal half 2 weeks later. The control group only received multi-agent chemotherapy. All patients were required to have cytological confirmation of high-grade lymphoma and achieve complete remission after two cycles of multi-agent chemotherapy. Fourteen patients receiving HBI and 11 patients in the control group were included. The median progression-free interval (PFI) in the HBI group (1143 days) was significantly longer than that in the control group (316 days, p = 0.004). In the HBI group, dogs with T cell lymphoma had statistically shorter PFI (292 days) than dogs with B cell lymphoma (2127 days, p = 0.0013). The median survival time in the HBI group (1924 days) was significantly longer than that in the chemotherapy-only group (566 days, p = 0.0077). The predictive factors for longer PFI and ST were found in the patients who received HBI and chemotherapy (p = 0.0062 and 0.0252, respectively). For chemotherapy-responding patients that completed a multi-agent protocol, HBI significantly prolonged the time to tumour relapse compared with the chemotherapy-only group.","PeriodicalId":23693,"journal":{"name":"Veterinary and comparative oncology","volume":" ","pages":""},"PeriodicalIF":2.3,"publicationDate":"2025-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143634701","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Contrast-Enhanced Computed Tomography for Identification of Potential Malignancy in Canine Colorectal Mesenchymal Tumours. 对比度增强计算机断层扫描用于鉴定犬结肠直肠间质瘤的潜在恶性程度。

IF 2.3 2区农林科学

Veterinary and comparative oncology Pub Date : 2025-03-08 DOI: 10.1111/vco.13047

Alexandria D Bourgeois, Owen T Skinner, James J Karnia, Amanda C Smith, Dae Young Kim, Aitor Gallastegui, Maureen A Griffin, Meaghan O'Neill, Brandan G Wustefeld-Janssens, Gabrielle S Fontes, Laura E Selmic, Marine Traverson, Carlos H de M Souza, Judith Bertran, Brian Thomsen, Arathi Vinayak, Megan A Mickelson

{"title":"Contrast-Enhanced Computed Tomography for Identification of Potential Malignancy in Canine Colorectal Mesenchymal Tumours.","authors":"Alexandria D Bourgeois, Owen T Skinner, James J Karnia, Amanda C Smith, Dae Young Kim, Aitor Gallastegui, Maureen A Griffin, Meaghan O'Neill, Brandan G Wustefeld-Janssens, Gabrielle S Fontes, Laura E Selmic, Marine Traverson, Carlos H de M Souza, Judith Bertran, Brian Thomsen, Arathi Vinayak, Megan A Mickelson","doi":"10.1111/vco.13047","DOIUrl":"10.1111/vco.13047","url":null,"abstract":"Cross-sectional imaging may be used to characterise the location and extent of colorectal mesenchymal tumours (CRMTs). Given the anticipated variation in tumour behaviour and varying morbidity based on surgical margins, a reliable, non-invasive means of predicting malignant potential could facilitate case management. The purpose of this multi-institutional, retrospective study was to determine the diagnostic accuracy of contrast-enhanced CT for distinguishing benign and malignant CRMTs. Twenty-seven dogs with CRMTs were included. Initial diagnoses were reviewed, and slides or blocks were available for 24/27 dogs for further histologic review and immunohistochemical labelling for smooth muscle actin, KIT and vimentin. Two masked radiologists reviewed DICOM images for tumour characteristics, including a final, binary, consensus, subjective interpretation of malignancy. Eighteen tumours (66.7%) were classified as leiomyoma, one (3.7%) as a benign other non-lymphogenic intestinal mesenchymal tumour (benign), one (3.7%) as leiomyosarcoma, and seven (25.9%) as gastrointestinal stromal tumour (malignant). Agreement between radiologists ranged from none to weak for categorical variables, with no agreement (κ = 0.135) for the final assessment of a tumour as benign or malignant. Substantial overlap was noted between groups, with no single categorical variable demonstrating high accuracy as a predictor of malignancy. Consensus final assessment was a sensitive (80.0%) but not specific (29.4%) predictor of malignancy (accuracy: 48.2%). No association was identified between CT determination of malignancy and histologic determination of malignancy (p = 0.678). Non-standardised contrast-enhanced CT was ineffective at distinguishing malignant from benign CRMTs in this study.","PeriodicalId":23693,"journal":{"name":"Veterinary and comparative oncology","volume":" ","pages":""},"PeriodicalIF":2.3,"publicationDate":"2025-03-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143582369","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Newcastle Disease Virus Induces Pyroptosis in Canine Mammary Tumour CMT-U27 Cells via the TNFα/NF-κB/NLRP3 Signalling Pathway.

IF 2.3 2区农林科学

Veterinary and comparative oncology Pub Date : 2025-03-03 DOI: 10.1111/vco.13048

Mengqing Li, Yunjie Zhu, Meng Li

{"title":"Newcastle Disease Virus Induces Pyroptosis in Canine Mammary Tumour CMT-U27 Cells via the TNFα/NF-κB/NLRP3 Signalling Pathway.","authors":"Mengqing Li, Yunjie Zhu, Meng Li","doi":"10.1111/vco.13048","DOIUrl":"https://doi.org/10.1111/vco.13048","url":null,"abstract":"Mammary tumours are the most common type of neoplasm in female dogs, with nearly half being malignant. Oncolytic Newcastle disease virus (NDV) therapy has emerged as a novel cancer treatment option; however, its precise oncolytic mechanism in canine mammary tumours (CMT) remain unclear. Ultrastructural analysis of NDV-infected CMT-U27 cells with locally damaged cell membranes and swollen and ruptured mitochondria revealed the occurrence of pyroptosis. Transcriptome sequencing further identified a significant upregulation of pyroptosis-related genes, including NLRP1, NOD2, caspase-1, and GSDMD. Subsequent examination of RNA and protein expression levels of pyroptosis-related molecules in vitro indicated that NDV induces pyroptosis in CMT-U27 cells via the caspase-1/GSDMD pathway. Additionally, inhibition of the TNFα/NF-κB pathway and knockdown of NOD-like receptor pyrin domain-containing protein 3 (NLRP3) using small interfering RNA demonstrated that the TNFα/NF-κB pathway can regulate NDV-induced pyroptosis through the NLRP3 inflammasome. In a xenograft model, intravenous administration of NDV significantly inhibited tumour growth, and prolonged the survival time in nude mice bearing CMT-U27 cells. NDV treatment enhances intratumoural pyrotosis in tumour bearing mice. In conclusion, these findings suggest that NDV induces pyroptosis in CMT-U27 cells through the TNFα/NF-κB/NLRP3 pathway, providing a foundation for future research into NDV's therapeutic potential in canine mammary cancer.","PeriodicalId":23693,"journal":{"name":"Veterinary and comparative oncology","volume":" ","pages":""},"PeriodicalIF":2.3,"publicationDate":"2025-03-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143543698","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Outcomes and Perioperative Complications of Surgical Resection on Canine Carotid Paragangliomas (21 Cases): A Veterinary Society of Surgical Oncology Study. 犬颈动脉旁神经节瘤手术切除的结果和围手术期并发症（21 例）：兽医肿瘤外科学会的一项研究。

IF 2.3 2区农林科学

Veterinary and comparative oncology Pub Date : 2025-03-01 Epub Date: 2024-11-04 DOI: 10.1111/vco.13023

Kathryn Marie Paskoff, Brad Matz, Quentin Fournier, Josep Aisa, Michelle L Oblak, Jonathan Bray, Daniel Linden

{"title":"Outcomes and Perioperative Complications of Surgical Resection on Canine Carotid Paragangliomas (21 Cases): A Veterinary Society of Surgical Oncology Study.","authors":"Kathryn Marie Paskoff, Brad Matz, Quentin Fournier, Josep Aisa, Michelle L Oblak, Jonathan Bray, Daniel Linden","doi":"10.1111/vco.13023","DOIUrl":"10.1111/vco.13023","url":null,"abstract":"Carotid body paragangliomas represent an uncommon neoplasm in dogs. The objective of this study was to report outcomes and complications associated with surgical excision of carotid body paragangliomas in 21 dogs. Cases were recruited retrospectively via medical record review from 9 veterinary speciality centres. The perioperative complication rate was 52% (11/21). Complications encountered in this cohort related to removal of carotid body tumour included airway obstruction, aspiration pneumonia, megaesophagus, unilateral laryngeal paralysis, coughing and Horner's syndrome. The overall perioperative mortality rate was 4.7% (1/21 dogs) and median survival time was 554 days for the six patients with known dates of death. One- and two-year survival rates were 61% and 42%, respectively. This is the largest collection of carotid body paraganglioma cases reported in veterinary literature. Based on these results, surgical resection of carotid body paragangliomas was associated with low perioperative mortality and long survival times.","PeriodicalId":23693,"journal":{"name":"Veterinary and comparative oncology","volume":" ","pages":"37-41"},"PeriodicalIF":2.3,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142576827","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0