Veterinary and comparative oncology最新文献

Performance of Frozen Section Histopathology, Imprint Cytology and Fine-Needle Aspirates for Detecting Canine Metastatic Mast Cell Tumour. 冷冻切片组织病理学、印迹细胞学和细针抽吸检测犬转移性肥大细胞瘤的性能。

IF 1.9 2区农林科学

Veterinary and comparative oncology Pub Date : 2025-10-02 DOI: 10.1111/vco.70023

Alejandro Alvarez-Sanchez, Katy L Townsend, Elena Gorman, Milan Milovancev, Duncan S Russell

{"title":"Performance of Frozen Section Histopathology, Imprint Cytology and Fine-Needle Aspirates for Detecting Canine Metastatic Mast Cell Tumour.","authors":"Alejandro Alvarez-Sanchez, Katy L Townsend, Elena Gorman, Milan Milovancev, Duncan S Russell","doi":"10.1111/vco.70023","DOIUrl":"https://doi.org/10.1111/vco.70023","url":null,"abstract":"Intra-operative staging of canine mast cell tumour (MCT) currently relies on routine cytology to determine nodal metastasis. While frozen section nodal histopathology is commonly used in humans, its applicability to veterinary settings is poorly characterised. The goal of this study was to determine the diagnostic performance of frozen section (FS) histopathology for diagnosing metastatic MCT, as compared to a formalin-fixed histopathologic gold standard. Performances of imprint cytology (IC) and fine needle aspirates (FNA) were also evaluated. Forty-one lymph nodes from 20 dogs with MCT were collected and stained with haematoxylin and eosin (HE) and Giemsa (formalin-fixed and frozen tissues), and Wright Giemsa and toluidine blue (IC and FNA). Nineteen out of 20 primary tumours were low grade. Frozen HE sections had poor agreement as compared to formalin-fixed HE histopathology (κ = 0.15); however, diagnostic performance increased to a good level of agreement when interpretation was combined with Giemsa (κ = 0.46). FNA and IC using Wright Giemsa had agreement comparable to combined frozen section histopathology (κ = 0.51 and 0.43, respectively). Combined frozen sections had a sensitivity of 65% and specificity of 93%, which was the same as FNA. Challenges encountered in morphologic interpretation of frozen sections included inadequate sectioning quality, architectural disruption, ruptured cells, and background metachromatic staining. These data provide support for FS histopathology as a feasible strategy for intra-operative detection of metastatic MCT, with diagnostic agreement similar to conventional cytology. Performance of FS histopathology is conditional upon a metachromatic stain evaluated in parallel with HE.","PeriodicalId":23693,"journal":{"name":"Veterinary and comparative oncology","volume":" ","pages":""},"PeriodicalIF":1.9,"publicationDate":"2025-10-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145207764","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Abstracts from the ESVONC Annual Congress, 22-24 May 2025. ESVONC年会摘要，2025年5月22-24日。

IF 1.9 2区农林科学

Veterinary and comparative oncology Pub Date : 2025-10-01 DOI: 10.1111/vco.70016

引用次数: 0

Clinical Response to Imatinib Mesylate and Toxicity Profile in 35 Dogs With Mast Cell Tumours. 甲磺酸伊马替尼对35只肥大细胞肿瘤犬的临床疗效及毒性分析

IF 1.9 2区农林科学

Veterinary and comparative oncology Pub Date : 2025-09-24 DOI: 10.1111/vco.70022

Elisabetta Treggiari, Emanuela Catania, Paola Valenti

{"title":"Clinical Response to Imatinib Mesylate and Toxicity Profile in 35 Dogs With Mast Cell Tumours.","authors":"Elisabetta Treggiari, Emanuela Catania, Paola Valenti","doi":"10.1111/vco.70022","DOIUrl":"https://doi.org/10.1111/vco.70022","url":null,"abstract":"Mast cell tumours (MCTs) are common in dogs. Treatment options include surgery, radiation therapy, and cytotoxic chemotherapy; however, in cases of inoperable or metastatic tumours, tyrosine kinase inhibitors (TKIs) can be used. Imatinib mesylate has been used in the treatment of solid tumours in both human and veterinary medicine. Previous studies have shown some efficacy in dogs with MCTs; however, additional data are needed to better define the optimal dosage, toxicity profile, and clinical outcomes associated with its use. Dogs with a cytological or histopathological diagnosis of mucosal, cutaneous or subcutaneous MCTs were included. Medical records from 2017 to 2024 were reviewed for clinical presentation, results of staging procedures, diagnostic tests, and treatment. Inclusion required the presence of macroscopic disease and administration of imatinib, either as a sole treatment or in combination with surgery. Thirty-five cases were included, all receiving medical treatment with or without surgical excision. Imatinib was administered as first-line treatment in 8 dogs (22.8%) and as a rescue treatment in 27 dogs (77.1%), achieving an overall clinical benefit, including complete response, partial response, and stable disease of 77%. Median progression-free survival was 37 days (range 13-770 days), while the overall median survival time (MST) was 270 days (range 83-1396 days). Following imatinib treatment, the MST was 105 days (range 22-1145 days). Gastrointestinal and haematological adverse events were recorded in 2 (5.7%) and 3 (8.6%) dogs, respectively, and were self-limiting. Imatinib treatment was generally well tolerated, with measurable clinical responses observed and only a limited spectrum of toxicities. Further studies are warranted to better characterise its safety and efficacy in dogs with MCTs.","PeriodicalId":23693,"journal":{"name":"Veterinary and comparative oncology","volume":" ","pages":""},"PeriodicalIF":1.9,"publicationDate":"2025-09-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145138989","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Favourable Prognostic Significance of HER2 Mutations in Canine Pulmonary Adenocarcinoma Following Surgical Resection. 犬肺腺癌手术切除后HER2突变对预后的有利意义。

IF 1.9 2区农林科学

Veterinary and comparative oncology Pub Date : 2025-09-04 DOI: 10.1111/vco.70020

Masanao Ichimata, Yumiko Kagawa, Atsushi Toshima, Masaya Igase, Takuya Mizuno

{"title":"Favourable Prognostic Significance of HER2 Mutations in Canine Pulmonary Adenocarcinoma Following Surgical Resection.","authors":"Masanao Ichimata, Yumiko Kagawa, Atsushi Toshima, Masaya Igase, Takuya Mizuno","doi":"10.1111/vco.70020","DOIUrl":"https://doi.org/10.1111/vco.70020","url":null,"abstract":"Human epidermal growth factor receptor 2 (HER2) gene mutations have been reported in 5% to 38% of canine pulmonary adenocarcinomas (cPACs), most commonly as V659E mutations in exon 20. However, their prognostic and predictive significance remains unclear. This retrospective, single-centre cohort study investigated the frequency of HER2 mutations in surgically resected cPACs and their association with clinical outcomes. Between 2005 and 2021, lung masses histologically diagnosed as cPACs were collected and subjected to direct sequencing of HER2 exons 20 and 21. A total of 72 dogs were enrolled, with successful HER2 gene analysis in 69 cases. HER2 exon 20 missense mutations were identified in 20 dogs (29.0%), including 18 harbouring the previously reported V659E hotspot mutation within the transmembrane domain. Homozygous mutations were detected in 13 dogs. Univariable analysis revealed associations between progression-free interval (PFI) and clinical signs, tumour size classification, lymph node metastasis, surgical margin status, and histologic grade. Overall survival time (OST) was associated with age, clinical signs, tumour size > 7 cm, histologic subtype, lymph node metastasis, and margin status. In multivariable analysis, tumour size classification and margin status remained significantly associated with PFI, while age, tumour size > 7 cm, and histologic subtype were independently associated with OST. Notably, the presence of HER2 mutations was significantly associated with prolonged PFI in both univariable and multivariable analyses, although no significant association with OST was observed. These findings suggest that HER2 mutation status may serve as a favourable prognostic marker for disease progression in surgically resected cPACs.","PeriodicalId":23693,"journal":{"name":"Veterinary and comparative oncology","volume":" ","pages":""},"PeriodicalIF":1.9,"publicationDate":"2025-09-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144993462","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Prognostic Value of a Histopathological Scoring System and the Ki67 Proliferation Index in Dogs With Phaeochromocytoma. 组织病理学评分系统和Ki67增殖指数对嗜铬细胞瘤犬的预后价值。

IF 1.9 2区农林科学

Veterinary and comparative oncology Pub Date : 2025-09-03 DOI: 10.1111/vco.70021

Marit F van den Berg, Aitor Martínez Ruiz, Maurice M J M Zandvliet, Sebastiaan A van Nimwegen, Hans S Kooistra, Sara Galac, Guy C M Grinwis

{"title":"Prognostic Value of a Histopathological Scoring System and the Ki67 Proliferation Index in Dogs With Phaeochromocytoma.","authors":"Marit F van den Berg, Aitor Martínez Ruiz, Maurice M J M Zandvliet, Sebastiaan A van Nimwegen, Hans S Kooistra, Sara Galac, Guy C M Grinwis","doi":"10.1111/vco.70021","DOIUrl":"10.1111/vco.70021","url":null,"abstract":"Canine phaeochromocytomas (PCCs) are neuroendocrine tumours with malignant potential. Metastatic disease remains the sole definitive evidence of malignancy. Histopathological criteria to predict long-term survival have not been established in dogs. This study evaluated the reproducibility and prognostic value of histopathological parameters derived from human scoring systems, along with the Ki67 proliferation index (PI), in dogs after adrenalectomy for PCC. Tumour samples from 41 dogs were assessed by a veterinary pathologist and pathology resident. Of 10 histopathological parameters examined, only necrosis, tumour cell spindling, and extension into adipose tissue achieved sufficient inter- and intra-observer agreement (≥ 0.40) for inclusion in survival analyses, while Ki67 PI demonstrated excellent reproducibility (≥ 0.95). A composite histopathological score was generated by summing these three parameters and a dichotomised Ki67 PI (optimal cutoff 18%), as determined by ROC analysis. Among the 41 dogs, eight died within 2 weeks postoperatively, leaving 33 long-term survivors with four tumour-related events. Kaplan-Meier analysis showed significantly poorer survival (p < 0.001) in dogs with a high Ki67 PI (≥ 18%), whereas the composite score showed a borderline significant association with outcome in Cox regression (p = 0.056; hazard ratio 2.80). Overall, dogs surviving the immediate postoperative period demonstrated a favourable prognosis (mean overall survival of 2456 days). These findings suggest that, in this cohort with few tumour-related events, the dichotomised Ki67 PI alone may serve as a clinically applicable prognosticator for canine PCC. However, further research in larger populations is needed to determine whether a composite score adds prognostic value and guides postoperative management.","PeriodicalId":23693,"journal":{"name":"Veterinary and comparative oncology","volume":" ","pages":""},"PeriodicalIF":1.9,"publicationDate":"2025-09-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144971222","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Analysis of Ki67 Density Maps Reveals Variation of Spatial Proliferation Patterns in Different Canine Neoplasms. Ki67密度图分析揭示不同犬肿瘤空间增殖模式的差异。

IF 1.9 2区农林科学

Veterinary and comparative oncology Pub Date : 2025-09-02 DOI: 10.1111/vco.70019

Brigandì Elena, Bacci Barbara, Brunetti Barbara, Rigillo Antonella, Roccabianca Paola, Bellini Giorgia, Chiappelli Chiara, Avallone Giancarlo

{"title":"Analysis of Ki67 Density Maps Reveals Variation of Spatial Proliferation Patterns in Different Canine Neoplasms.","authors":"Brigandì Elena, Bacci Barbara, Brunetti Barbara, Rigillo Antonella, Roccabianca Paola, Bellini Giorgia, Chiappelli Chiara, Avallone Giancarlo","doi":"10.1111/vco.70019","DOIUrl":"https://doi.org/10.1111/vco.70019","url":null,"abstract":"Mitotic count (MC) is a well-established prognostic factor in many canine malignancies. While standardisation efforts have improved inter-pathologist agreement regarding the morphology of mitotic figures and the size of the counting area, the selection of the tumour region for MC assessment remains to be standardised. This study aimed to evaluate the spatial distribution of the most proliferative areas in selected canine tumour types, using Ki67 immunohistochemistry, to identify optimal candidate regions for MC assessment. Tumour types analysed included melanomas, cutaneous mast cell tumours (cMCT), canine mammary carcinomas (CMC) and soft tissue sarcomas (STS). Using image analysis, Ki67 density maps were generated from digital slides and classified according to their distribution pattern (focal/multifocal or diffuse) and location within the tumour (central, peripheral or scattered). A total of 202 cases were included: 43 melanomas, 30 cMCTs, 42 CMCs and 87 cSTSs. The vast majority of tumours (92.6%) exhibited a multifocal hotspot distribution. Peripheral hotspot localisation was predominant in 55% of cases, particularly in cMCTs (73.3%) and melanomas (76.7%). In contrast, cSTSs more frequently showed a scattered hotspot pattern (60.9%) (χ2 = 41.9; p < 0.001). CMCs had a higher proportion of centrally located hotspots (16.7%). These findings suggest that pathologists should focus on peripheral tumour regions when assessing MC in cMCTs and melanomas. Both central and peripheral regions should be considered in CMCs, while a more extensive, comprehensive evaluation may be required in STSs. The observed association between tumour histotype and proliferation pattern likely reflects inherent biological differences among the tumour types studied.","PeriodicalId":23693,"journal":{"name":"Veterinary and comparative oncology","volume":" ","pages":""},"PeriodicalIF":1.9,"publicationDate":"2025-09-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144971088","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Expanding the Spectrum of Canine Diffuse Large B-Cell Lymphoma Genetic Aberrations Through Whole Genome Sequencing Analysis. 通过全基因组测序分析扩大犬弥漫性大b细胞淋巴瘤遗传畸变谱。

IF 1.9 2区农林科学

Veterinary and comparative oncology Pub Date : 2025-09-01 Epub Date: 2025-05-04 DOI: 10.1111/vco.13059

Antonella Fanelli, Eugenio Mazzone, Diana Giannuzzi, Laura Marconato, Luca Aresu

{"title":"Expanding the Spectrum of Canine Diffuse Large B-Cell Lymphoma Genetic Aberrations Through Whole Genome Sequencing Analysis.","authors":"Antonella Fanelli, Eugenio Mazzone, Diana Giannuzzi, Laura Marconato, Luca Aresu","doi":"10.1111/vco.13059","DOIUrl":"10.1111/vco.13059","url":null,"abstract":"Diffuse large B-cell lymphoma (DLBCL) is one of the most prevalent haematological malignancies in both humans and dogs, characterised in both species by significant clinical heterogeneity and limited prognostic predictability. With the introduction of next-generation sequencing (NGS) technologies in veterinary medicine over the past decade, researchers have begun to elucidate the molecular basis of canine DLBCL (cDLBCL); however, much of the clinical heterogeneity associated with this tumour remains unexplained. In this study, we performed whole genome sequencing on 10 cDLBCL cases, all treated with chemo-immunotherapy, which exhibited similar clinico-pathological features but markedly different outcomes. Cases were classified as \"poor\" or \"good\" responders based on whether their lymphoma-specific survival fell below or above the cohort's median. Protein-coding variants and copy number aberrations unique to poor or good responders revealed novel candidate genes not previously identified in cDLBCL studies, while splicing, untranslated regions, and intronic variants were detected in genes already known to be recurrently mutated. In conclusion, our investigation has broadened the spectrum of potentially pathogenic variants implicated in cDLBCL, though further studies with larger cohorts are necessary to validate these findings.","PeriodicalId":23693,"journal":{"name":"Veterinary and comparative oncology","volume":" ","pages":"346-357"},"PeriodicalIF":1.9,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12378081/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144029573","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Prognostic Role of Patient, Tumour and Radiomic Factors Influencing Outcomes in Dogs With Thyroid Cancer Treated With Iodine-131. 患者、肿瘤和放射学因素对碘-131治疗甲状腺癌犬预后的影响

IF 1.9 2区农林科学

Veterinary and comparative oncology Pub Date : 2025-09-01 Epub Date: 2025-06-04 DOI: 10.1111/vco.13070

Ibrahim Chamseddine, Caitlin Cowan, Lindsay Donnelly, Rebecca J Abergel, Jan Schuemann, Alejandro Bertolet, Charles A Maitz

{"title":"Prognostic Role of Patient, Tumour and Radiomic Factors Influencing Outcomes in Dogs With Thyroid Cancer Treated With Iodine-131.","authors":"Ibrahim Chamseddine, Caitlin Cowan, Lindsay Donnelly, Rebecca J Abergel, Jan Schuemann, Alejandro Bertolet, Charles A Maitz","doi":"10.1111/vco.13070","DOIUrl":"10.1111/vco.13070","url":null,"abstract":"Radioactive iodine therapy (I-131) is widely used in both human and veterinary medicine for treating thyroid cancer, utilising the common biological behaviour of thyroid tissues. Recognising the need to better understand the prognostic factors affecting heterogeneous treatment outcomes, this retrospective study analysed 32 dogs with thyroid carcinoma treated at the University of Missouri Veterinary Health Center. The study examined variables such as patient demographics, tumour characteristics, and first-order radiomic features. Purposeful feature selection was employed to identify both significant and confounding factors, with a focus on treatment response and survival time. Specific administered activity (SAA) emerged as a significant predictor of treatment response, with higher SAA levels improving odds of response (p value = 0.02, OR = 10.28), while clinical signs and nodal stage (N-stage) were identified as significant confounders. Moreover, metastasis stage (M-stage) (p < 0.01, OR = 0.35) and tumour-to-salivary gland dose uptake ratio (TSGR) significantly affected survival time (p = 0.02, OR = 1.57), with TSGR displaying a notable nonlinear effect. These results underline the importance of refined patient stratification and highlight the potential for personalised dose adjustments to enhance outcomes in canine thyroid cancer.","PeriodicalId":23693,"journal":{"name":"Veterinary and comparative oncology","volume":" ","pages":"454-464"},"PeriodicalIF":1.9,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12354336/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144226824","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Auranofin Induces ER Stress-Mediated Apoptosis, and Its Combination With Bortezomib Elicits Paraptosis-Like Cell Death in Malignant Canine Mammary Tumour Cells. 金嘌呤诱导内质网应激介导的细胞凋亡，并与硼替佐米联合诱导犬乳腺恶性肿瘤细胞凋亡样细胞死亡。

IF 1.9 2区农林科学

Veterinary and comparative oncology Pub Date : 2025-09-01 Epub Date: 2025-04-15 DOI: 10.1111/vco.13062

Yoon-Ho Suh, Se-Hoon Kim, Ki-Hoon Song, Jun-Yeol Choi, Min-Ok Ryu, Robert B Rebhun, Kyoung-Won Seo

{"title":"Auranofin Induces ER Stress-Mediated Apoptosis, and Its Combination With Bortezomib Elicits Paraptosis-Like Cell Death in Malignant Canine Mammary Tumour Cells.","authors":"Yoon-Ho Suh, Se-Hoon Kim, Ki-Hoon Song, Jun-Yeol Choi, Min-Ok Ryu, Robert B Rebhun, Kyoung-Won Seo","doi":"10.1111/vco.13062","DOIUrl":"10.1111/vco.13062","url":null,"abstract":"Canine mammary tumours (CMT) are common in female dogs, often associated with malignancy and limited responses to conventional therapies. This study explores the potential of Auranofin (AF) in malignant CMT, focusing on its ability to induce distinct cell deaths. AF inhibited thioredoxin reductase (TrxR) activity, cell proliferation, and colony formation across malignant CMT cell lines, demonstrating significant anticancer effects. In AF-sensitive cell lines (CMT-U27, CHMm, and CHMp), 0.5-2 μM AF induced endoplasmic reticulum (ER) stress-mediated apoptosis, while concentrations above 3 μM caused near-complete cell death via additional proteasome inhibition. However, in AF-resistant cell lines (CIPp and CIPm), AF concentrations required for near-complete cell death were higher, expected to be challenging to achieve clinically. Therefore, we combined sublethal doses of AF (~2 μM) with the proteasome inhibitor Bortezomib (Bz) in these cells. The combination exhibited synergistic cytotoxicity and induced extensive cytoplasmic vacuolation. Live-cell staining revealed the ER origin of vacuoles, and cycloheximide pretreatment inhibited both vacuolation and AF + Bz-induced cell death, indicating features of paraptosis. While apoptosis could not be excluded, it was classified as paraptosis-like cell death occurring concurrently with apoptosis. Further analysis supported that this cell death is related to enhanced ER stress from AF-induced TrxR inhibition and Bz-induced proteasome inhibition. Based on these findings, we propose AF alone or combined with Bz as a promising therapeutic strategy for malignant CMT. Our findings highlight AF's potential to induce ER stress-mediated apoptosis and paraptosis-like cell death in canine cancer cells, expanding therapeutic options for targeting cancers in dogs.","PeriodicalId":23693,"journal":{"name":"Veterinary and comparative oncology","volume":" ","pages":"377-387"},"PeriodicalIF":1.9,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12378087/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144039969","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Molecular Classification Based on the Gene Expression Profiles in Canine Histiocytic Sarcoma Cells. 基于犬组织细胞肉瘤细胞基因表达谱的分子分类。

IF 1.9 2区农林科学

Veterinary and comparative oncology Pub Date : 2025-09-01 Epub Date: 2025-06-11 DOI: 10.1111/vco.13071

Hiroki Sakuma, Hirotaka Tomiyasu, Akiyoshi Tani, Yuko Goto-Koshino, Makoto Bonkobara, Masaru Okuda

{"title":"Molecular Classification Based on the Gene Expression Profiles in Canine Histiocytic Sarcoma Cells.","authors":"Hiroki Sakuma, Hirotaka Tomiyasu, Akiyoshi Tani, Yuko Goto-Koshino, Makoto Bonkobara, Masaru Okuda","doi":"10.1111/vco.13071","DOIUrl":"10.1111/vco.13071","url":null,"abstract":"The molecular abnormalities of canine histiocytic sarcoma (CHS) remain to be elucidated. We previously revealed that the sensitivities to dasatinib and trametinib were significantly various among CHS cell lines, indicating the differences in underlying molecular abnormalities. In the present study, we performed RNA sequencing analysis using 11 CHS cell lines to investigate molecular classifications based on the gene expression profiles (GEPs). The clustering analysis showed that CHS cell lines were divided into two distinct clusters. The comparisons of GEPs between the clusters extracted 675 differentially expressed genes (DEGs), and these DEGs were enriched with those related to the regulations of inflammatory responses. Among these DEGs, differences in the expressions of CCL3, CCL4, CCL7, CLEC7A, and TLR4 genes between the two groups were confirmed by RT-qPCR. Since no significant difference in the activation status of Akt and ERK pathways was observed between the two groups, the NF-κB pathway was focused on and its activation status was examined in the cell lines. As a result, cell lines belonging to one cluster showed nuclear translocation of the p65 protein together with increased release of CCL5 protein, which is a target molecule of the NF-κB pathway, in a cell culture supernatant. These results suggested that the molecular pathology of CHS cells might be divided into two categories depending on the activation status of the NF-κB pathway, and it is necessary to establish precision medicine for each molecular subtype of CHS.","PeriodicalId":23693,"journal":{"name":"Veterinary and comparative oncology","volume":" ","pages":"465-475"},"PeriodicalIF":1.9,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12378086/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144275991","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0