Veterinary and comparative oncology最新文献_第4页

Combination of Hypofractionated Radiation Therapy and Gilvetmab for the Treatment of Cutaneous or Subcutaneous Mast Cell Tumours in Dogs. 低分割放疗联合Gilvetmab治疗犬皮肤或皮下肥大细胞瘤。

IF 1.8 2区农林科学

Veterinary and comparative oncology Pub Date : 2026-09-01 DOI: 10.1111/vco.70110

Tiffany W Martin, Douglas H Thamm, Kristen M Weishaar

{"title":"Combination of Hypofractionated Radiation Therapy and Gilvetmab for the Treatment of Cutaneous or Subcutaneous Mast Cell Tumours in Dogs.","authors":"Tiffany W Martin, Douglas H Thamm, Kristen M Weishaar","doi":"10.1111/vco.70110","DOIUrl":"https://doi.org/10.1111/vco.70110","url":null,"abstract":"Gilvetmab is a caninised monoclonal antibody against canine PD-1. Concurrent or sequential administration of anti-PD-1 antibodies and radiation therapy (RT) improves survival time in various species. Eighteen dogs with mast cell tumours (MCTs) were prospectively treated with 24 Gy of RT over Days 0, 7 and 21 and gilvetmab every 2 weeks beginning on the second fraction of RT (Day 7) for up to 10 doses. Best tumour response was complete in one (6%), partial in eight (44%) and stable disease in nine dogs (50%) for an ORR of 50%. Median time to best response was 7 days after the first fraction of RT. Thirteen dogs (72%) developed progressive disease with a median progression free interval (PFI) of 116 days after the first fraction of RT. Nine adverse events (AEs) attributed to gilvetmab were recorded in seven dogs (39%) consisting of infusion reactions in four, metabolic changes in three and gastrointestinal signs and pancreatitis in one each. Overall, five dogs (28%) experienced Grade 3 AEs. Radiation AEs were limited to Grade 1 for both acute and late effects in seven dogs (39%). There was a significant difference in PFI and MST for dogs with and without lymph node metastasis with a median PFI of 64 versus 292 days and MST of 103 versus 587 days (p = 0.02 and 0.005, respectively). This sequence, or combination, of therapies did not provide durable tumour control for canine MCT and the AE profile warrants further evaluation.","PeriodicalId":23693,"journal":{"name":"Veterinary and comparative oncology","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148875889","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Proteasome Inhibitor-Induced Cytotoxic Mechanisms in Canine Pulmonary Adenocarcinoma Cell Lines. 蛋白酶体抑制剂诱导犬肺腺癌细胞的细胞毒性机制。

IF 1.8 2区农林科学

Veterinary and comparative oncology Pub Date : 2026-09-01 Epub Date: 2026-04-06 DOI: 10.1111/vco.70065

Aika Chijiwa, Sakuya Inanaga, Tomohiro Osaki, Masaya Igase, Takuya Mizuno

{"title":"Proteasome Inhibitor-Induced Cytotoxic Mechanisms in Canine Pulmonary Adenocarcinoma Cell Lines.","authors":"Aika Chijiwa, Sakuya Inanaga, Tomohiro Osaki, Masaya Igase, Takuya Mizuno","doi":"10.1111/vco.70065","DOIUrl":"10.1111/vco.70065","url":null,"abstract":"Canine pulmonary adenocarcinoma (cPAC) lacks well-validated systemic treatment options, motivating preclinical identification of potential therapeutic candidates. To explore potential therapeutic candidates, we first performed a screening of an FDA-approved drug library using a cPAC cell line, which identified bortezomib-a proteasome inhibitor-as a potential agent. Guided by this result, we focused subsequent investigations on proteasome blockade and evaluated the antitumor activity and mechanisms of bortezomib together with a second-generation proteasome inhibitor, carfilzomib-alone and in combination with standard cytotoxics-in three cPAC cell lines (CLAC, HDC, and LuBi). Both agents reduced cell proliferation and cell viability in a dose-dependent manner, with proteasome target engagement (ubiquitinated protein accumulation), G2/M arrest, sub-G1 increase, and caspase-3 cleavage. Pan- and selective caspase inhibition partially rescued viability, suggesting contributions from both intrinsic and extrinsic apoptosis, together with caspase-independent mechanisms. We also observed activation of the JNK-c-Jun pathway and increased Bim expression, indicating the induction of cellular stress responses and apoptosis. Drug-interaction analysis showed reproducible synergy between carfilzomib and carboplatin in HDC and LuBi, whereas combinations with vinorelbine were mainly additive or antagonistic effects. Overall, proteasome inhibitors exert multifaceted cytotoxic effects in cPAC through proteotoxic stress, cell-cycle arrest, and caspase-associated apoptosis. In particular, the combination of carfilzomib and carboplatin may represent a promising therapeutic strategy. Further in vivo and clinical investigations are warranted to evaluate therapeutic efficacy, safety, and pharmacokinetics of proteasome inhibitors in cPAC.","PeriodicalId":23693,"journal":{"name":"Veterinary and comparative oncology","volume":" ","pages":"421-432"},"PeriodicalIF":1.8,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13456618/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147628733","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Integrative Phosphoproteomic Profiling Reveals Stage-Specific Signalling and Metabolism in Equine Melanocytic Neoplasm. 综合磷酸化蛋白质组学分析揭示马黑色素细胞肿瘤的阶段特异性信号传导和代谢。

IF 1.8 2区农林科学

Veterinary and comparative oncology Pub Date : 2026-09-01 Epub Date: 2026-04-16 DOI: 10.1111/vco.70070

Paitoon Srimontri, Amornthep Kingkaw, Nawarus Prapaiwan, Rangsima Sujittosakul, Nichapat Iamkaewprasert, Jiraschaya Piputwat, Puetta Isama-Al, Thanutchanok Munkongdee, Thanapon Chotikaprakal, Petchpailin Yanyongsirikarn, Narumon Phaonakrop, Sittiruk Roytrakul, Wanwipa Vongsangnak, Parichart Tesena

{"title":"Integrative Phosphoproteomic Profiling Reveals Stage-Specific Signalling and Metabolism in Equine Melanocytic Neoplasm.","authors":"Paitoon Srimontri, Amornthep Kingkaw, Nawarus Prapaiwan, Rangsima Sujittosakul, Nichapat Iamkaewprasert, Jiraschaya Piputwat, Puetta Isama-Al, Thanutchanok Munkongdee, Thanapon Chotikaprakal, Petchpailin Yanyongsirikarn, Narumon Phaonakrop, Sittiruk Roytrakul, Wanwipa Vongsangnak, Parichart Tesena","doi":"10.1111/vco.70070","DOIUrl":"10.1111/vco.70070","url":null,"abstract":"Equine melanocytic neoplasms (EMN) are aggressive tumours characterised by high metastatic potential and limited therapeutic options available. However, the molecular mechanisms underlying their progression remain poorly understood. This study therefore presents the integrative phosphoproteomic analysis of EMN tissue, with the aim of elucidating stage-specific alterations in signalling pathways and metabolism. Nineteen tissue samples from grey horses were categorised as normal-stage (n = 6), early-stage EMN (n = 7), and severe-stage EMN (n = 6) and subjected to in-depth analysis using liquid chromatography-tandem mass spectrometry (LC-MS/MS). A total of 2035 phosphoproteins were identified, of which 219 were differentially expressed across the disease stages. Interestingly, early-stage EMN showed dysregulation of inositol phosphate metabolism and activation of the PI3K-Akt pathway which involved INPP5F and PKN2. In severe-stage EMN, upregulation of SYNJ1, STRN4 and VIM indicated enhanced membrane trafficking, cytoskeletal remodelling, and MAPK signalling. Additionally, ASPM and GNAO1 upregulation reflected heightened proliferation and altered Rap1 signalling, while UBR5 dysregulation suggested aberrant protein homeostasis. Metabolic reprogramming was also noticed, with elevated TKT and GAPDH expression supporting glycolysis and NADPH production. Observably, the severe-stage EMN exhibited a higher expression of Dickkopf-3 (DKK3) which suggests a role in aberrant Wnt/β-catenin activation and tumour progression. These findings reveal stage-specific molecular mechanisms in EMN pathogenesis and highlight potential biomarkers and therapeutic targets for equine melanoma.","PeriodicalId":23693,"journal":{"name":"Veterinary and comparative oncology","volume":" ","pages":"468-484"},"PeriodicalIF":1.8,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13456603/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147700172","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Retrospective Analysis of Survival and Adverse Effects Associated With Stereotactic Body Radiation Therapy in 12 Dogs With Hepatocellular Carcinoma. 12只肝细胞癌犬立体定向放射治疗的生存和不良反应回顾性分析。

IF 1.8 2区农林科学

Veterinary and comparative oncology Pub Date : 2026-09-01 Epub Date: 2026-04-19 DOI: 10.1111/vco.70071

Victoria Lee Newberry, Mary-Keara Boss, Del Leary, Tiffany W Martin

{"title":"Retrospective Analysis of Survival and Adverse Effects Associated With Stereotactic Body Radiation Therapy in 12 Dogs With Hepatocellular Carcinoma.","authors":"Victoria Lee Newberry, Mary-Keara Boss, Del Leary, Tiffany W Martin","doi":"10.1111/vco.70071","DOIUrl":"10.1111/vco.70071","url":null,"abstract":"This retrospective analysis evaluated 12 dogs with hepatocellular carcinoma (HCC) who received stereotactic body radiation therapy (SBRT). Dogs received between 6 and 25 Grey (Gy) per fraction for 1-5 fractions for a total dose of 13-30 Gy. Eleven patients (92%) experienced acute adverse effects (AE), with the majority being VRTOG grade 2-3 stomach/small intestinal AE. Additionally, 70% of patients experienced late AE, with the majority being VRTOG grade 2 stomach/small intestinal AE. Liver-specific side effects were also observed following SBRT, including VCOG-CTCAE grade 4 hepatic encephalopathy and grade 5 liver failure. There was a statistically significant correlation between the gross tumour volume (GTV) and the overall survival time (OST; p = 0.02) as well as a significant correlation between the percentage of normal liver affected by the mass and the severity of acute stomach/small intestinal AE (p = 0.01). Statistically significant correlations were observed between the dose of radiation to the normal liver, starting with a threshold dose of 7 Gy, and an increase of the liver enzyme ALT. Median survival time (MST) of the cohort was 301 days. Dogs with > 28.7% of the liver affected by the mass had a MST of 182 days vs. 419 days for dogs with < 28.7% affected (p = 0.009). This study suggests parameters for GTV, percentage of liver affected, and dose to normal liver could affect survival and the likelihood for adverse effects and liver enzyme elevation when treating with SBRT.","PeriodicalId":23693,"journal":{"name":"Veterinary and comparative oncology","volume":" ","pages":"485-498"},"PeriodicalIF":1.8,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13456606/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147723706","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Abstracts from the 2026 Veterinary Cancer Society (VCS) Annual Conference, 24-26^th September 2026. 2026年兽医癌症协会（VCS）年会摘要，2026年9月24-26日。

IF 1.8 2区农林科学

Veterinary and comparative oncology Pub Date : 2026-09-01 DOI: 10.1111/vco.70098

引用次数: 0

Prognostic Significance of the Tumour Microenvironment in Surgically Resected Canine Pulmonary Adenocarcinomas. 手术切除犬肺腺癌肿瘤微环境的预后意义。

IF 1.8 2区农林科学

Veterinary and comparative oncology Pub Date : 2026-09-01 Epub Date: 2026-04-27 DOI: 10.1111/vco.70075

Masanao Ichimata, Yumiko Kagawa, Atsushi Toshima, Fukiko Matsuyama, Eri Fukazawa, Kei Harada, Ryuzo Katayama, Yuko Nakano, Tetsuya Kobayashi, Masaya Igase, Takuya Mizuno

{"title":"Prognostic Significance of the Tumour Microenvironment in Surgically Resected Canine Pulmonary Adenocarcinomas.","authors":"Masanao Ichimata, Yumiko Kagawa, Atsushi Toshima, Fukiko Matsuyama, Eri Fukazawa, Kei Harada, Ryuzo Katayama, Yuko Nakano, Tetsuya Kobayashi, Masaya Igase, Takuya Mizuno","doi":"10.1111/vco.70075","DOIUrl":"10.1111/vco.70075","url":null,"abstract":"The prognostic relevance of the tumour microenvironment in surgically resected canine pulmonary adenocarcinomas (cPACs) remains uncertain. This retrospective single-centre cohort study evaluated associations between intratumoural immune features and postoperative outcomes. Dogs treated between 2005 and 2021 with histologically confirmed cPACs that had undergone surgical excision and had an evaluable tumour microenvironment were included. Immunohistochemistry for CD8, Foxp3, CD3 and CD20 was performed. CD8+ and Foxp3+ cell densities were quantified in five intratumoural hotspots of 0.237 mm2 each. Lymphoid aggregates (LAs) were defined as intratumoural aggregates located within tumour nests or intratumoural stroma and composed of at least 50 CD20+ B cells with intercellular spacing ≤ 10 μm and admixed CD3+ T cells. Primary outcomes were progression-free interval (PFI) and overall survival time (OST). A total of 71 dogs were enrolled. LAs were identified in 49 dogs (69.0%). The median densities of CD8+, CD20+ and Foxp3+ cells were 130.0, 398.3 and 79.3 cells/mm2, respectively. Tumours with LAs showed higher CD20+ and Foxp3+ cell densities than tumours without LAs. Median PFI and OST were 754 days (95% CI, 375-not reached) and 716 days (95% CI, 399-936), respectively. In multivariable analysis, tumour size classification, lymph node metastasis, surgical margin status and the presence of LAs were independently associated with shorter PFI. Age, lymph node metastasis and the CD8/Foxp3 ratio were independently associated with OST. These findings indicate that immunohistochemical features may provide additional prognostic information for postoperative risk stratification in cPACs and complement established clinicopathological factors.","PeriodicalId":23693,"journal":{"name":"Veterinary and comparative oncology","volume":" ","pages":"545-553"},"PeriodicalIF":1.8,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13456590/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147782943","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Plasma Dimethylarginine Concentrations in Dogs With Large Cell Lymphoma or Hemangiosarcoma: A Prospective Observational Study. 大细胞淋巴瘤或血管肉瘤犬血浆二甲基精氨酸浓度：一项前瞻性观察研究。

IF 1.8 2区农林科学

Veterinary and comparative oncology Pub Date : 2026-08-27 DOI: 10.1111/vco.70108

Grégoire Bernardo Marques, Christelle Maurey, Delphine Le Roux, Jérémy Béguin

{"title":"Plasma Dimethylarginine Concentrations in Dogs With Large Cell Lymphoma or Hemangiosarcoma: A Prospective Observational Study.","authors":"Grégoire Bernardo Marques, Christelle Maurey, Delphine Le Roux, Jérémy Béguin","doi":"10.1111/vco.70108","DOIUrl":"https://doi.org/10.1111/vco.70108","url":null,"abstract":"Asymmetric dimethylarginine (ADMA) and symmetric dimethylarginine (SDMA) are endogenous methylated arginine derivatives associated with endothelial dysfunction and tumour progression in humans. Their relevance in canine oncology remains unclear. This prospective study aimed to assess plasma concentrations of ADMA and SDMA in dogs newly diagnosed with large cell lymphoma or non-cutaneous hemangiosarcoma (HSA); dogs with benign splenic lesions were also enrolled. Renal function was assessed at the time of inclusion to exclude overt kidney disease. Plasma ADMA and SDMA concentrations were measured at diagnosis, and clinical staging data were collected. A total of 31 dogs were enrolled: 11 with lymphoma, 15 with HSA and 5 with benign splenic lesions. SDMA concentrations exceeded the reference interval in 47%, 36% and 0% of dogs with HSA, lymphoma and benign lesions, respectively. Dogs with lymphoma (p = 0.003) and HSA (p = 0.013) had significantly higher SDMA concentrations than dogs with benign splenic lesions. Moreover, dogs with presumed disseminated HSA had significantly higher SDMA concentrations than those without metastatic disease (median: 21.5 vs. 12 μg/dL; p = 0.012). In contrast, plasma ADMA concentrations did not differ significantly among groups (p = 0.39). These findings suggest that lymphoma and HSA may represent extra-renal causes of SDMA elevation in dogs, and that SDMA could have value as a biomarker of tumour burden or progression. The potential clinical utility of ADMA in canine cancer warrants further investigation.","PeriodicalId":23693,"journal":{"name":"Veterinary and comparative oncology","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2026-08-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148841331","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Clinical Behaviour and Outcome of Pelvic Osteosarcoma in Dogs: A Retrospective Multicentre Analysis of 74 Cases (2007-2025). 犬盆腔骨肉瘤的临床行为和预后：74例回顾性多中心分析（2007-2025）。

IF 1.8 2区农林科学

Veterinary and comparative oncology Pub Date : 2026-08-22 DOI: 10.1111/vco.70106

Csilla Fejös, Felicitas Czichon, Maureen Griffin, Natalie Grillo, Quentin Fournier, Michelle L Oblak, Mikaela Eng-Toogood, Michael Kent, Isabelle Desmas-Bazelle, Miriam Kleiter, Laura Marconato, Katie McNaught, Simona Vincenti, Carmit Chalfon, Petros Odatzoglou, Carla Rohrer Bley

{"title":"Clinical Behaviour and Outcome of Pelvic Osteosarcoma in Dogs: A Retrospective Multicentre Analysis of 74 Cases (2007-2025).","authors":"Csilla Fejös, Felicitas Czichon, Maureen Griffin, Natalie Grillo, Quentin Fournier, Michelle L Oblak, Mikaela Eng-Toogood, Michael Kent, Isabelle Desmas-Bazelle, Miriam Kleiter, Laura Marconato, Katie McNaught, Simona Vincenti, Carmit Chalfon, Petros Odatzoglou, Carla Rohrer Bley","doi":"10.1111/vco.70106","DOIUrl":"https://doi.org/10.1111/vco.70106","url":null,"abstract":"Although osteosarcoma is the most common primary bone tumour in dogs, involvement of the pelvic bones is rare. The aim of the present study was to describe clinical presentation, identify possible prognostic factors, and evaluate outcomes in dogs diagnosed with pelvic osteosarcoma. Seventy-four dogs with cytologically or histologically diagnosed pelvic osteosarcoma were included in this multicentre retrospective study (2007-2025). The median progression-free survival (PFS) and overall survival (OS) were estimated using the Kaplan-Meier method, with comparisons performed using log-rank tests. Prognostic factors for PFS and OS were assessed using multivariable Cox proportional hazards regression analysis. Lameness (84%) was the most common presenting clinical sign, and the ilium (57%) was the most frequent intrapelvic tumour location. Thirty-one percent of dogs had metastases at diagnosis, primarily affecting the lungs. The median PFS was 75 days, and the median OS was 79 days. Dogs receiving any treatment had significantly longer PFS (116 vs. 7 days, p < 0.001) and OS (145 vs. 8 days, p < 0.001) than untreated dogs. No factors were significantly associated with PFS. In contrast, dogs with higher body weight and shorter time to diagnosis tended to have more favourable survival, whereas chemotherapy and local progression were associated with poorer outcomes. Despite the overall poor prognosis associated with pelvic osteosarcoma, treatment may extend survival. An individualized, often multimodal approach may provide clinical benefit in selected dogs.","PeriodicalId":23693,"journal":{"name":"Veterinary and comparative oncology","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2026-08-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148798404","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Toxicity of Cytarabine Intravenous Constant Rate Infusion in Cats With Lymphoma. 阿糖胞苷恒速静脉输注对淋巴瘤猫的毒性。

IF 1.8 2区农林科学

Veterinary and comparative oncology Pub Date : 2026-08-18 DOI: 10.1111/vco.70107

Rachel Hanford, Katherine A Skorupski, Robert B Rebhun, Rachel V Brady, Sami Al-Nadaf, Amandine T Lejeune

{"title":"Toxicity of Cytarabine Intravenous Constant Rate Infusion in Cats With Lymphoma.","authors":"Rachel Hanford, Katherine A Skorupski, Robert B Rebhun, Rachel V Brady, Sami Al-Nadaf, Amandine T Lejeune","doi":"10.1111/vco.70107","DOIUrl":"https://doi.org/10.1111/vco.70107","url":null,"abstract":"Cytarabine intravenous constant rate infusion (IV CRI) toxicity in cats with lymphoma has not been well documented. We aimed to assess toxicities following cytarabine IV CRI as a single-agent or within a multiagent protocol in cats with lymphoma. Medical records of cats with lymphoma treated with cytarabine IV CRI, with documented dose, route, duration and at least one follow-up visit between 1990 and 2024, were retrospectively reviewed. Adverse events (AEs) were retrospectively graded according to VCOG-CTCAE v2. Thirty-eight cats were included, and 70 doses were evaluated for AEs. Median cytarabine dose was 389.8 mg/m2 (range 100.1-697.3 mg/m2) over a median infusion duration of 6 h (range 2-17 h). A total of 103 AEs were documented across 70 administrations. Gastrointestinal AEs were the most common category: altered appetite (21/38 cats, 55.2%), vomiting (9/38, 23.7%; one grade 3, the rest grades 1-2), diarrhoea (2/38, 5.3%) and dysphagia (2/38, 5.3%). Anaemia was the most common hematologic AE (14/38 cats, 36.8%; five grade 1, two grade 2, five grade 3, two grade 4); six cats (15.8%) developed thrombocytopenia (four grade 1, two grade 2), and one cat (2.6%) developed grade 2 neutropenia. Lethargy was the most common constitutional AE (12/38 cats, 31.6%). IV CRI of cytarabine was generally tolerated, with predominantly low-grade AEs, although grade 3-4 anaemia was not uncommon. Response rate and clinical benefit remain unknown; investigation of alternative dosing regimens may help clarify its therapeutic potential.","PeriodicalId":23693,"journal":{"name":"Veterinary and comparative oncology","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2026-08-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148798369","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Immunohistochemical Characterisation of Mismatch Repair Proteins in Feline Cancers: A Pilot Study Using Validated Cross-Reactive Antibodies. 猫癌症中错配修复蛋白的免疫组织化学特征：一项使用有效交叉反应抗体的初步研究。

IF 1.8 2区农林科学

Veterinary and comparative oncology Pub Date : 2026-08-16 DOI: 10.1111/vco.70105

Shoma Nishibori, Masashi Sakurai, Yumiko Kagawa, Kazuo Nishigaki, Takayuki Nakagawa, Sakuya Inanaga, Masaya Igase, Takuya Mizuno

{"title":"Immunohistochemical Characterisation of Mismatch Repair Proteins in Feline Cancers: A Pilot Study Using Validated Cross-Reactive Antibodies.","authors":"Shoma Nishibori, Masashi Sakurai, Yumiko Kagawa, Kazuo Nishigaki, Takayuki Nakagawa, Sakuya Inanaga, Masaya Igase, Takuya Mizuno","doi":"10.1111/vco.70105","DOIUrl":"https://doi.org/10.1111/vco.70105","url":null,"abstract":"DNA mismatch repair (MMR) deficiency is a clinically important biomarker in human oncology, yet its relevance in feline neoplasia remains poorly understood due to limited characterisation and the absence of validated reagents. In this study, we established a practical immunohistochemistry (IHC) approach for evaluating feline MMR proteins by confirming the cross-reactivity of anti-human MLH1, MSH2 and MSH6 antibodies using CRISPR/Cas9-engineered feline tumour cell lines lacking each target gene. Using these validated antibodies, we conducted a pilot screening to assess MMR protein expression in 83 feline tumours and observed variable reductions or losses of MLH1, MSH2 and MSH6 across several tumour types. Notably, MLH1 expression was lost in all melanoma cases (6/6, 100%) and in most osteosarcomas (14/15, 93%), indicating that MMR perturbations may be particularly relevant in these malignancies. These findings provide the first analytically validated framework for assessing MMR proteins in feline oncology and suggest that MMR dysregulation may be more widespread than previously recognised. Although the clinical sample size is limited, this initial pilot screening maps the preliminary landscape of feline MMR protein expression. Further large-scale validation studies incorporating microsatellite instability testing, methylation analysis and genomic profiling are warranted to clarify the biological significance of these alterations.","PeriodicalId":23693,"journal":{"name":"Veterinary and comparative oncology","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2026-08-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148761578","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0