Veterinary Research Communications最新文献

{"title":"Effects of zinc oxide and monoglyceride blend on intestinal immune, health and microbiota responses of weanling pigs.","authors":"Ana Lígia Braga Mezzina, Erick Marlon Pereira, Cecília Archangelo Ferreira de Melo, Thallysson Taumaturgo Silva Souza, Matheus Saliba Monteiro, André Pegoraro Poor, Bruno Bracco Donatelli Muro, Caroline Veloso, Flávio de Aguiar Coelho, Rafaella Fernandes Carnevale, Francisco Alves Pereira, Fernanda Mariane Dos Santos, Jose Alfonso Echavarria Martínez, Rodrigo Knop Guazzi Messias, Cesar Augusto Pospissil Garbossa","doi":"10.1007/s11259-025-10762-4","DOIUrl":"https://doi.org/10.1007/s11259-025-10762-4","url":null,"abstract":"<p><p>Organic acids are increasingly being investigated as alternatives to zinc oxide (ZnO) in nursery pig diets. Monoglycerides can act in distal parts of the intestine, promoting intestinal health, also reducing bacterial fermentation in hindgut. This study aimed to determine the effects of dietary supplementation based on a blend of monoglycerides (C4:C8:C10) (MBLEND) in replacement to ZnO during the nursery phase on growth-performance and intestinal health. Ninety-six piglets were used (average weight 7.08 ± 0.837 kg) with 25 days of age during 42 days, divided into three treatments and eight replicates in a randomized block design. The treatments were: CON - Negative control (basal diet without additive supplementation); MBLEND - Fatty acids (basal diet with monoglycerides blend (C4:C8:C10) additive supplementation at 1.5 kg/ton in the pre-initial 1 and pre-initial 2 phases, 1.0 kg/ton in the initial 1 and initial 2 phases) and ZnO - Zinc oxide (basal diet with zinc oxide supplementation at 3000 ppm in the pre-initial 1, pre-initial 2 and initial 1 phases, 2000 ppm in the initial 2 phase). Both ZnO and MBLEND groups did not influence (P>0.05) growth performance during all phases. The inclusion of the MBLEND improved (P<0.05) dry matter (DM), crude protein (CP), and ashes digestibility when compared to CON and ZnO groups. The use of ZnO reduced (P<0.05) IL-1 β cytokine expression in the piglets' jejunal mucosa. ZnO increased evenness and affected beta-diversity (P<0.05) compared to CON and MBLEND. The ZnO group increased Tannerellaceae, Bacteroidaceae, and Muribaculaceae families and reduced Escherichia Coli-Shigella and Desulfovibrio generas (q<0.05). Although there were similar results on growth performance, ZnO modulated cecal microbiota and reduced inflammatory ileal cytokines and MBLEND improved protein and ashes digestibility.</p>","PeriodicalId":23690,"journal":{"name":"Veterinary Research Communications","volume":"49 4","pages":"197"},"PeriodicalIF":1.8,"publicationDate":"2025-05-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144036448","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Clinical and pathological characterization of a feline intestinal large granular lymphocyte lymphoma (LGLL).","authors":"Camila Issa Amaral, Lorena Santos Bezerra, Acácia Eduarda de Jesus Nascimento, Nayara Toledo da Silva, Lize Amanda Basaglia Borges, Paloma Helena Sanches da Silva, Letícia Monteiro Barbosa, Anelise Carvalho Nepomuceno, Rodrigo Dos Santos Horta, Geovanni Dantas Cassali, Ayisa Rodrigues Oliveira","doi":"10.1007/s11259-025-10765-1","DOIUrl":"https://doi.org/10.1007/s11259-025-10765-1","url":null,"abstract":"<p><p>A five-year-old neutered male domestic cat presented with a three-month history of vomiting, hyporexia, progressive weight loss, and abdominal pain. Ultrasound revealed a significant increase in the thickness of the ileum, suggestive of a neoplastic process. Enterectomy and enteroanastomosis was performed, and the sample was sent for histopathological examination. Grossly, the intestine was tortuous and was irregular. In the antimesenteric portion, a 1.4 cm thick, annular, homogenous, white and soft mass was present slightly reducing luminal diameter. Histologically, the neoplastic cells were arranged in sheets of large and pleomorphic round cells with numerous intracytoplasmic eosinophilic granules extending from the lamina propria to the serosa. Tumor cell immunolabelling was for CD3, multifocal for granzyme B and absent for CD79a and CD20. Based on the morphology and immunophenotyping of the neoplastic cells, the diagnosis of intestinal large granular lymphoma was made.</p>","PeriodicalId":23690,"journal":{"name":"Veterinary Research Communications","volume":"49 4","pages":"195"},"PeriodicalIF":1.8,"publicationDate":"2025-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144030143","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dietary supplementation with açai (Euterpe oleracea) improved the haemato-immunological parameters and growth performance of koi carp (Cyprinus Carpio Linnaeus, 1758).","authors":"Alexandre Vaz da Silva, Emilly Monteiro Lopes, Domickson Silva Costa, Gracienhe Gomes Dos Santos, Caio Francisco Santana Farias, Paola Capistrano Dos Santos, Ana Paula de Souza, Gabriele Bueno Dos Anjos, Manoela Clemente Fernandes, Arlene Sobrinho Ventura, Claudia Andrea Lima Cardoso, Marco Shizuo Owatari, José Luiz Pedreira Mouriño, Maurício Laterça Martins","doi":"10.1007/s11259-025-10763-3","DOIUrl":"https://doi.org/10.1007/s11259-025-10763-3","url":null,"abstract":"<p><p>Amazonian phytotherapeutics show great potential in treating various diseases and have proven effective in aquaculture. Açai, a plant rich in antioxidants, essential fatty acids, and anti-inflammatory properties, is a promising feed additive for fish. This study evaluated the effects of dietary supplementation with açai in the diet of the juvenile koi carp Cyprinus carpio (Koi variety). For this purpose, the fish were subjected to five dietary treatments with different amounts of açai in the feed [0, 5, 10, 15 and 20 g kg^-1] for 60 days. Phytochemical analyses revealed that the contents of phenolic compounds, flavonoids, tannins, and carotenoids and the antioxidant potential of the experimental diets increased as the inclusion volume increased. At the end of the feed trial, the growth performance, haematoimmunological parameters, and chemical analysis of the fillet and skin of the fish were evaluated. Weight gain, daily weight gain, biomass gain, daily biomass gain, and feed conversion were significantly greater (p < 0.05) in the 15 g kg^-1 group than in the 5 g kg^-1 and control groups. In terms of haematology, the number of thrombocytes was significantly greater (p < 0.05) in the fish from the 20 g kg^-1 treatment group than in those from the control group. The plasma antimicrobial activity significantly increased (p < 0.05) in the 15 g kg^-1 and 20 g kg^-1 treatments, whereas the agglutination titre was significantly positive (p < 0.05) in the açai treatments compared with the control group. The results indicated that freeze-dried açai pulp could be used as a growth promoter and improve the innate immune response of C. carpio after 60 days of dietary supplementation.</p>","PeriodicalId":23690,"journal":{"name":"Veterinary Research Communications","volume":"49 4","pages":"193"},"PeriodicalIF":1.8,"publicationDate":"2025-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144050641","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Zoonotic relevance of multidrug-resistant bacteria in parrots with respiratory illness.","authors":"Ahmed Samir, Tarek Mosallam, Hassan Aboul-Ella, Aisha Ali, Ojena Samir, Mohamed Hegab, Mark Erian, Fady Youssef, Hala Zaher","doi":"10.1007/s11259-025-10752-6","DOIUrl":"https://doi.org/10.1007/s11259-025-10752-6","url":null,"abstract":"<p><p>Nowadays, research attention is paid to the investigation of bacterial pathogens in the cloaca of parrots rather than the nasal niche, which is largely ignored. Therefore, this study aimed to investigate the nasal carriage of multidrug-resistant bacteria with zoonotic potential in parrots suffering from respiratory illness. Nasal swabs were collected from 75 sick parrots with respiratory illness, and they were subjected to microbiological isolation and identification, followed by antimicrobial susceptibility testing. Escherichia coli, Klebsiella pneumoniae, Proteus mirabilis, and Staphylococcus aureus were isolated with a prevalence rate of 36%, 32%, 26.7%, and 9.3%, respectively, while one isolate (1.3%) of Staphylococcus pseudointermedius, Staphylococcus simulans, Staphylococcus sciuri, and Enterococcus faecalis was identified. E. coli, K. pneumoniae, and P. mirabilis were investigated for ESBL genes, Staphylococcus species for the mecA gene, followed by SCCmec typing, and E. faecalis for the vanA and vanB genes. Regarding beta-lactamase-encoding genes, bla_TEM (97.6%), bla_SHV (48.8%), and bla_CTX-M (39%) gene families were detected, while bla_OXA was not found. Sequencing of bla_CTX-M in one strain of E. coli, K. pneumoniae, and P. mirabilis revealed bla_CTX-M-15. The mecA was determined in three S. aureus and one S. sciuri strain, and the SCCmec typing of three MRSA isolates yielded type V, whereas type I in S. sciuri. Only the vanA gene was recognized in the E. faecalis strain. Moreover, 67.1% of bacterial isolates exhibited multidrug resistance. These findings highlight the potential role of parrots in the transmission of multidrug-resistant zoonotic bacteria, which may pose a threat to human contacts.</p>","PeriodicalId":23690,"journal":{"name":"Veterinary Research Communications","volume":"49 4","pages":"194"},"PeriodicalIF":1.8,"publicationDate":"2025-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12062053/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144001361","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Approaching equine infectious anemia in regions dominated by grade-working animals - a case of Pará, a state in the Brazilian Amazon.","authors":"Ana Paula Vilhena Beckman Pinho, Fernando Ferreira, Jeferson Jacó Fuck, Jefferson Pinto de Oliveira, Ricardo Augusto Dias, José Henrique Hidebrand Grisi-Filho, Marcos Bryan Heinemann, Evelise Oliveira Telles, Marcos Amaku, José Soares Ferreira Neto","doi":"10.1007/s11259-025-10759-z","DOIUrl":"https://doi.org/10.1007/s11259-025-10759-z","url":null,"abstract":"<p><p>Considering that control strategies for Equine Infectious Anemia, based on the serological diagnosis of equids and the removal of positive animals, may not be optimal for developing countries with an absolute predominance of grade working animals, this study aimed to address this issue based on the epidemiological situation of the disease in Pará, a state in the Brazilian Amazon. Pará was divided into five regions, and within each region a pre-established number of farms were randomly selected. Within each farm, a pre-set number of animals were randomly selected and submitted to Agar Gel Immunodiffusion test. A questionnaire was administered on the farms to identify risk factors for the disease. In total, 2,718 equids (horses, mules and donkeys) from 654 farms were tested. The prevalence at farms and animal level in the state was 34.5% (95% CI: 30.8-38.4) and 15.8% (95% CI: 14.4-17.4), with significant regional differences. Equids from farms with 11 or more equids (OR = 2.32 [95% CI: 1.38-3.92]) and those that shared water sources with other farms (OR = 1.76 [95% CI: 1.26-2.46]) were more likely to be infected. The average sensitivity of the surveillance system for detecting infected farms ranged from 0.92 to 1.14%, which is insufficient to disrupt the endemic balance of the disease in the state, demanding a reassessment. The key elements of this process, as well as the potential strategies to be implemented, have been discussed in the context of Pará and can be extended to regions with similar characteristics.</p>","PeriodicalId":23690,"journal":{"name":"Veterinary Research Communications","volume":"49 4","pages":"192"},"PeriodicalIF":1.8,"publicationDate":"2025-05-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143988739","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Survival and viability of Toxoplasma gondii oocysts under natural dry season conditions in the Brazilian semi-arid region.","authors":"Ana Luzia Peixoto Silva, Brendo Andrade Lima, Victor Hugo Alves Sousa Formiga, Estefany Ferreira Lima, Geraldo Moreira Silva Filho, Welitânia Inácia Silva, Jordania Oliveira Silva, Felipe Boniedj Ventura Alvares, Vinícius Longo Ribeiro Vilela, Thais Ferreira Feitosa","doi":"10.1007/s11259-025-10757-1","DOIUrl":"https://doi.org/10.1007/s11259-025-10757-1","url":null,"abstract":"<p><p>Due to the outbreaks of toxoplasmosis in Brazil, primarily caused by oocysts, the objective of this study was to evaluate how Toxoplasma gondii oocysts behave in the Brazilian semi-arid region and the influence of environmental conditions on the occurrence of outbreaks in the region. Cats were infected with mouse brains containing T. gondii cysts and subjected to five different environmental conditions: Group 1: Positive fecal samples exposed to sunlight and covered with 2 cm of common sand; Group 2: Positive fecal samples exposed to sunlight on the surface of the sand; Group 3: Positive fecal samples in the shade, covered with 2 cm of common sand; Group 4: Positive fecal samples in the shade on the surface of the sand; Group 5: Positive fecal samples kept under ideal temperature and humidity conditions in Biochemical Oxygen Demand (BOD) - Control. Samples were collected initially after 12 h of environmental exposure and subsequently every 12 h to evaluate the presence of oocysts and the sporulation rate. Temperature and humidity were monitored throughout the experiment. After 24 h of exposure, the oocysts were completely destroyed, and 92% of the oocysts were sporulated within 12 h of initial exposure. Soil temperatures reached up to 57 °C, with humidity levels as low as 15%. The study concludes that high temperatures and low humidity are decisive factors in the destruction of T. gondii oocysts, which are rapidly inactivated when exposed to the semi-arid climate of Northeastern Brazil during the dry season.</p>","PeriodicalId":23690,"journal":{"name":"Veterinary Research Communications","volume":"49 4","pages":"191"},"PeriodicalIF":1.8,"publicationDate":"2025-05-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143988742","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Histopathological and immunohistochemical characteristics of chordae tendineae affected by degenerative processes in canine myxomatous mitral valve disease.","authors":"Justyn Gach, Agnieszka Mackiewicz, Izabela Janus-Ziółkowska, Agnieszka Noszczyk-Nowak","doi":"10.1007/s11259-025-10761-5","DOIUrl":"https://doi.org/10.1007/s11259-025-10761-5","url":null,"abstract":"<p><p>Myxomatous mitral valve disease is a major problem in canine cardiology. Degenerative changes extend from the valve leaflets to reach the rest of the subvalvular apparatus. The chordae tendineae (CT) play a key role in the mechanics of the mitral valve and ensure unidirectional blood flow through the heart. Degenerative changes within the chordae tendineae can severely disrupt their function, ultimately leading to an episode of chordae rupture. The study aimed to analyse the structure of healthy and degenerated CTs via histopathology and immunohistochemistry. The mitral valve was assessed macroscopically using the Whitney scale to identify degenerative changes. The chordae tendineae were classified on a four-grade scale (0-3) on the basis of structural changes and subsequently analysed through immunohistochemical staining with antibodies targeting collagens I, III, and IV, as well as fibronectin, chondroitin, and tenascin. The findings revealed alterations in the extracellular matrix in degenerated chordae tendineae.</p>","PeriodicalId":23690,"journal":{"name":"Veterinary Research Communications","volume":"49 4","pages":"190"},"PeriodicalIF":1.8,"publicationDate":"2025-05-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12055879/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144052664","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Clinical, pathological, and molecular aspects of malignant catarrhal fever in Russia: a case report.","authors":"Pavel A Andriyanov, Olga I Zakharova, Pavel A Zhurilov, Anastasia I Tutrina, Daria D Kashina, Olga A Burova, Elena A Liskova, Ekaterina A Shirokova, Ivan V Yashin, Andrey A Blokhin","doi":"10.1007/s11259-025-10758-0","DOIUrl":"https://doi.org/10.1007/s11259-025-10758-0","url":null,"abstract":"<p><p>Malignant catarrhal fever (MCF) is a highly fatal viral disease caused by ovine gammaherpesvirus-2 (OvHV-2) that primarily affects cattle around the world. Sheep act as asymptomatic carriers, silently perpetuating the virus. Despite its global presence, MCF remains poorly understood in Russia due to limited surveillance, voluntary disease reporting, and its exclusion from routine differential diagnoses. This study aimed to investigate a suspected case of MCF in a Yakut-breed cow on a private farm in Pskov Oblast, Russia, bordering Estonia. The cow resided in a mixed sheep and cattle herd with direct sheep contact. Clinical examination and histological analysis revealed characteristic MCF lesions. Phylogenetic analysis of the cow's OvHV-2 revealed a mosaic structure. The polymerase01, gene clustered with strains from the Netherlands and Germany, while the tegument gene grouped with isolates from India and Egypt. This highlights the global nature of OvHV-2 genetic diversity. Overall, this study provides the first documented case of MCF in Russia and underscores the sporadic nature of the disease in cattle. The identification of novel pathological changes and the unique viral genetic makeup necessitate further investigation of MCF in Russia.</p>","PeriodicalId":23690,"journal":{"name":"Veterinary Research Communications","volume":"49 4","pages":"189"},"PeriodicalIF":1.8,"publicationDate":"2025-05-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144017110","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"DNA porcine viruses detected on fresh liver samples destined for human consumption.","authors":"Mariana Antunes Rezende, Yasmin Ampese Maté, Jean Francisco Maldaner Lui, Ana Clara Seibel, Rafael Frandoloso, Luiz Carlos Kreutz","doi":"10.1007/s11259-025-10753-5","DOIUrl":"https://doi.org/10.1007/s11259-025-10753-5","url":null,"abstract":"<p><p>Pork meat and pork-derived products are major sources of protein, but they might be important vehicles for transmitting viruses to humans. Here we aimed to evaluate whether pig enzootic viruses could be found in liver samples destined for foodstuff manufacture. We collected 140 liver samples in a slaughterhouse for DNA extraction and viral detection by real-time or conventional PCR. The samples were from 14 swine herds (n = 10/herd) located in Rio Grande do Sul state, Brazil. We found 93 (66.4%) samples positive for swine torque teno virus (TTSuV); out of these, 29 samples (31.2%) were positive for TTSuV1, 39 (41.9%) for TTSuV2 and 25 (26.9%) for both TTSuV genotypes. Porcine circovirus type 2 (PCV2) DNA was found on 24 (17.1%) samples, out of which 23 were PCV2d and only one was PCV2b. Half of the samples from each farm were tested for adenoviruses (AdV) and 44 (62.8%) were positive: by analyzing the melting curve, the samples were classified as adenoviruses of human (HAdV), canine (CAdV), and bird (AVAdV) origin in 39 (55.7%), 11 (15.7%) and 3 (4.2%) of the samples. Porcine parvovirus (PPV) DNA was detected in 5 (7.1%) of the 70 samples analyzed. Pork liver is commonly used for making pate, sausages, and mortadella which, in this situation, could harbor these viruses. Further processing of foodstuff is likely to inactive viral agents; however, our data indicates that pork-derived meat might contain viruses that eventually could reach the food chain and be harmful to immunocompromised individuals.</p>","PeriodicalId":23690,"journal":{"name":"Veterinary Research Communications","volume":"49 4","pages":"188"},"PeriodicalIF":1.8,"publicationDate":"2025-05-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144050642","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mycological and molecular identification of mycoses involved in mastitis from Holstein dairy cattle with special reference to Candida albicans.","authors":"Hayat Fayed, Asmaa A Elgendy, Salma A Shoulah, Samar M Moustafa, Ahmed Maher, Ahmed F Hikal, Ahmed Abdeen, Ekramy Elmorsy, Mohamed E Mohamed, Helal F Hetta, Heba Allah Elbaghdady, Afrina Mustari, Samah F Ibrahim, Ateya M Ibrahim, Marawan A Marawan","doi":"10.1007/s11259-025-10744-6","DOIUrl":"https://doi.org/10.1007/s11259-025-10744-6","url":null,"abstract":"<p><p>Globally, mastitis is an incredibly devastating, multifactorial disease that affects the dairy industry. However, cases of mycotic mastitis in dairy cows have increased in recent years. This study aimed to investigate the prevalence of mycotic mastitis with special reference to Candida albicans in Holstein-Friesian dairy cows from some farms in Menoufia province, Egypt. For the study, 150 milk samples (n = 150, 20 from healthy cows and 130 from cows with subclinical and clinical mastitis) were collected from 15 smallholder dairy farms located in three localities (Quesina, Elbagour, and Shibin El-Kom) five farms from each. The samples were subjected to mycological culture and subsequently verified using traditional morphological and biochemical tests to confirm fungal characteristics followed by antimycotic susceptibility testing using Vitec 2 system. Finally, molecular identification was carried out via PCR and phylogenetic analysis. 27 samples out of 130 were positive for fungal culture representing (20.77%). Candida species were recorded the highest percentage (62.96%) of the detected fungi (17/27). In addition, C. albicans was the predominant species (3/27), which corresponds to 11.11%. Antifungal susceptibility testing of the fungal isolates showed the highest susceptibility to micafungin, followed by caspofungin (88.88% and 86.19%, respectively). However, the lowest susceptibility was noted against amphotericin B (50.55%). The three isolates of C. albicans were confirmed by PCR by amplifying the ITS region, which was then sequenced for phylogenetic analysis. The sequences have been deposited in GenBank under the following accession numbers: OR97266, OR97267, and OR97268. Our results indicate that Candida spp., especially C. albicans, is one of the major causes of mycotic mastitis in dairy cows in Egypt. Moreover, micafungin might be the efficient medication for treating these cases of mycotic mastitis, followed by caspofungin.</p>","PeriodicalId":23690,"journal":{"name":"Veterinary Research Communications","volume":"49 4","pages":"187"},"PeriodicalIF":1.8,"publicationDate":"2025-05-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144064889","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}