Veterinary Research Communications最新文献

{"title":"Expression of chicken circovirus Cap protein and establishment of ELISA method for antibody detection.","authors":"Man Xu, Tingting Zhang, Weiyu Gao, Xinling Xu, Haoyan Yang, Hao Wang, Bin Xie, Xiang Liu, Baoshan Liu, Zeliang Chen","doi":"10.1007/s11259-024-10603-w","DOIUrl":"10.1007/s11259-024-10603-w","url":null,"abstract":"<p><p>A recently identified virus, chicken circovirus (ChCV), has been linked to the onset of acute gastroenteritis in chicks, a condition that can have a detrimental impact on the overall health and well-being of chickens in a farming setting. In this study, we developed an enzyme-linked immunosorbent assay (ELISA) method for the indirect detection of antibodies against the chicken circovirus (ChCV) through codon optimization, which effectively expressed the capsid protein of the ChCV and utilized it as an encapsulated antigen following purification. In establishing the ELISA method for detecting antibodies using the purified Cap protein as the antigen, the optimal concentration of the antigen was determined to be 1 µg/mL, the optimal blocking solution was identified as 1% bovine serum albumin, the optimal dilution ratio of the serum to be tested was established to be 1:100, and the dilution ratio of the secondary antibody was determined to be 1:5,000. At these thresholds, the sensitivity of the ELISA method was 94.44%, and the specificity was 100%. The testing of 203 clinical samples yielded a positivity rate of 46.8%, indicating that the virus is endemic in chickens. In conclusion, this study established an ELISA method to detect antibodies against chicken circovirus using recombinant Cap protein as antigen, demonstrating good specificity and sensitivity. This lays the foundation for the development of related kits and the detection of infection and epidemiology of chicken circovirus. Meanwhile, the analysis concluded that chicken circovirus infection is more common, and the prevention and control of this disease should be emphasised and strengthened.</p>","PeriodicalId":23690,"journal":{"name":"Veterinary Research Communications","volume":"49 1","pages":"34"},"PeriodicalIF":1.8,"publicationDate":"2024-11-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142710202","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Smartphone-based ECG assessment of heart rhythm at birth in healthy foals born after spontaneous or induced parturition: is there a potential difference?","authors":"Marina Petrucelli, Micaela Sgorbini, Carolina Castagnetti, Aliai Lanci, Jole Mariella, Paola Marmorini, Francesca Freccero","doi":"10.1007/s11259-024-10576-w","DOIUrl":"https://doi.org/10.1007/s11259-024-10576-w","url":null,"abstract":"<p><p>Background- Subclinical electrophysiological derangements occur at birth in foals. Smartphone-based ECG (spECG) is practical tools to assess heart rhythm and was recently validated in foals. Objectives-To assess the heart rate (HR) and rhythm at birth in clinically healthy foals born after spontaneous and induced parturition using a spECG. Animals- Newborn healthy foals born after at term spontaneous (SF) or induced foaling (IF). Methods- Cohort study. In SF and IF groups, a spECG was performed immediately at birth (T0) and after 5 min (T5). SpECG tracings were selected for quality. HR was manually calculated and was compared between groups and time-points. Tracings were manually interpreted for rhythm diagnosis. Results- Eighty-one spECG tracings owing to 42 healthy foals (36 SF, 6 IF) were of adequate quality. There were no significant differences in HR between SF and IF and between T0 and T5 (p > 0.05). Six foals were diagnosed with atrial fibrillation and 4 with sinus arrhythmia. Conclusions and clinical importance- The spECG was feasible and detected subclinical arrhythmias at birth. A potential relationship with induced foaling warrants confirmation. Provided good quality tracing is obtained, the device might assist in the clinical assessment at birth, especially in screening high-risk foals.</p>","PeriodicalId":23690,"journal":{"name":"Veterinary Research Communications","volume":"49 1","pages":"31"},"PeriodicalIF":1.8,"publicationDate":"2024-11-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142695346","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Limosilactobacillus reuteri B1/1 modulated the intestinal immune response in preventing Salmonella Enteritidis PT4 infection in a chicken ileal explant model.","authors":"Viera Karaffová, Zuzana Kiššová, Csilla Tóthová, Patrik Tráj, Máté Mackei, Gábor Mátis","doi":"10.1007/s11259-024-10609-4","DOIUrl":"10.1007/s11259-024-10609-4","url":null,"abstract":"<p><p>In this study, we observed the effect of the newly isolated probiotic strain Limosilactobacillus reuteri B1/1 on the relative gene expression of selected cytokines (interleukin-15, transforming growth factor-β4), tight junction proteins (E-cadherin, occludin), biomarker active intestinal stem cells - LGR5 (leucine-rich repeat containing G protein-coupled receptor), markers of mucosal intestinal immunity (mucin-2, immunoglobulin A), as well as the creation of a new biomarker of inflammation in the intestine - calprotectin on an ex vivo model of chicken ileal explant in the prevention of Salmonella Enteritidis PT4 infection. The ability of L. reuteri B1/1 to effectively modulate the mucosal immune response under pretreatment conditions in S. Enteritidis PT4 infection in a chicken ileal explant model was confirmed. In addition, our obtained results point to the fact that the new chicken ileum explant model could be a suitable model to investigate or test the influence of natural substances such as probiotic bacteria in the interaction with the intestine as well as pathogenic microorganisms. In addition, the results of our study may contribute to a deeper understanding of the action of newly isolated probiotic bacteria at the intestinal level using ex vivo models such as chicken ileum explant, which are able to mimic in vivo conditions sufficiently.</p>","PeriodicalId":23690,"journal":{"name":"Veterinary Research Communications","volume":"49 1","pages":"32"},"PeriodicalIF":1.8,"publicationDate":"2024-11-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11585517/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142695150","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Treatment of mycotic rhinitis caused by aspergillus fumigatus in a quarter horse mare using topical clotrimazole and oral potassium iodide.","authors":"Juliana de Moura Alonso, Marcos Jun Watanabe, Sandra de Moraes Gimenes Bosco, Emanuel Vitor Pereira Apolonio, Artur Bibiano de Vasconcelos, Ana Carolina do Prado, Ana Liz Garcia Alves, Celso Antonio Rodrigues, Carlos Alberto Hussni","doi":"10.1007/s11259-024-10570-2","DOIUrl":"10.1007/s11259-024-10570-2","url":null,"abstract":"<p><p>Fungal rhinitis in horses is rare, although severe, due to the progressive nasal obstruction. Aspergillus is one of the fungi most often involved in these cases. A 7-year-old Quarter Horse mare weighing 325 kg was admitted with a history of recurrent nasal discharge and bleeding, foul smell, reluctance to exercise, and abortion. Laboratory and imaging examinations revealed eosinophilia, scarring and erosion areas in the nasal mucosa, purulent and bloody secretions, and remarkable yellowish plaques. Histopathological examination revealed fungal rhinitis and bacterial culture revealed the presence of β-hemolytic Streptococcus and Staphylococcus sp. Fungal culture and molecular diagnosis confirmed Aspergillus fumigatus as the etiological agent. A tracheostomy was necessary due to the patient's dyspnea. For the treatment, topical application of clotrimazole ointment was carried out in the rostral portion of the nasal cavity once a day and in the caudal portion of the nasal cavity, endoscopic guided every 48 h. In the fifth endoscopy-guided application, a significant improvement was noticed; however, the animal exhibited a hypersensitivity reaction, and the treatment was discontinued. Thus, potassium iodide therapy was initiated (67 mg/kg, PO, SID, 14 days). The patient demonstrated favorable clinical improvement, achieved resolution of the fungal infection, and was discharged after 25 days of hospitalization. Topical clotrimazole demonstrated a probable efficacy as a standalone treatment, although its use had to be prematurely discontinued due to the emergence of a hypersensitivity reaction. The combination of clotrimazole and potassium iodide effectively treated fungal rhinitis in a shorter time than previously reported. This report provides useful guidance for other cases of fungal rhinitis, although further studies are needed.</p>","PeriodicalId":23690,"journal":{"name":"Veterinary Research Communications","volume":"49 1","pages":"28"},"PeriodicalIF":1.8,"publicationDate":"2024-11-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142688930","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"CT findings and anatomical aspects of a Labrador retriver humerus characterized by osteophytosis of the sulcus intertubercularis and obliteration of the supratrochlear foramen.","authors":"Sedef Selviler-Sizer, Semih Kurt, Burcu Onuk, Murat Kabak","doi":"10.1007/s11259-024-10583-x","DOIUrl":"10.1007/s11259-024-10583-x","url":null,"abstract":"<p><p>Degenerative changes in the skeletal system of dogs may occur as a consequence of aging, trauma, infection, or inflammation. The object of this study consisted of an 8-year-old male Labrador dog that was brought to a private veterinary clinic after a road traffic accident and died despite all interventions. For a different study planned in our department, CT images were taken of the Labrador retriever's humerus and osteophytes to encounter incidentally were investigated for found in these images. After the CT scan, the dissected humerus bones were boiled to remove soft tissues. Subsequently, the bones were underwent with a 10% hydrogen peroxide solution for bleaching. Osteophytes in the proximal part of the humerus extended from the tuberculum majus and tuberculum minus towards the cranial direction, forming a complete ring-like structure at the level of the sulcus intertubercularis in the left humerus. Although not fully ring-shaped, similar osteophytes were found in the right humerus. In addition, it was observed that the foramen supratrochleare seen in the left humerus in the CT images was covered with a thin membrane during dissection. The presence of this membrane suggested that it might be due to the joint not being able to reach sufficient extension due to osteophytes forming in the bone. Additionally, it was concluded that CT may not recognize thin, non-mineralized septations, such as the membrane obliterating the supratrochlear foramen, as observed during necropsy.</p>","PeriodicalId":23690,"journal":{"name":"Veterinary Research Communications","volume":"49 1","pages":"27"},"PeriodicalIF":1.8,"publicationDate":"2024-11-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142688749","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Etiology and antimicrobial resistance of subclinical mastitis pathogens Staphylococcus aureus, Streptococcus spp. and Enterococcus spp. in sheep milk.","authors":"Ionica Iancu, Violeta Igna, Sebastian Alexandru Popa, Kálmán Imre, Corina Pascu, Luminița Costinar, Janos Degi, Alexandru Gligor, Vlad Iorgoni, Corina Badea, Ileana Nichita, Viorel Herman","doi":"10.1007/s11259-024-10579-7","DOIUrl":"10.1007/s11259-024-10579-7","url":null,"abstract":"<p><p>The present study investigates the prevalence and etiology of subclinical mastitis in Țurcana sheep flocks located in south-western Romania. Milchtest and California Mastitis Test (CMT), were used for the detection of subclinical mastitis. A number of 360 milk samples across three lactation phases were analyzed. Subclinical mastitis was identified in 146 (40.6%) of sheep milk samples. Only milk samples (n = 146) collected from sheep diagnosed with subclinical mastitis were considered for microbiological analyses. Thus, Staphylococcus aureus (129/146; 88.4%) was identified as the predominant pathogen followed by Streptococcus spp. (14/146; 9.6%) Enterococcus spp. (7/146; 4.8%), Pseudomonas aeruginosa (6/146; 4.1%) and Klebsiella pneumoniae (5/146; 3.4%). Antimicrobial susceptibility of isolated strains of Staphylococcus aureus, Streptococcus spp. and Enterococcus spp. was also tested. The results showed varying resistance patterns depending on the farm and microorganism. In heard A the highest resistance of Staphylococcus aureus was identified to polymyxin B (79.7%) followed by novobiocin (73.4%) and tetracycline (45.3%); Streptococcus spp. to streptomycin (100%), novobiocin (100%), tetracycline (100%) and Enterococcus spp. to tylosin (100%), streptomycin (75.0%) and tetracycline (75.0%). In herd B, Staphylococcus aureus showed high resistance rates to polymyxin B (86.3%), novobiocin (74.5%) and cloxacillin (39.2%); Streptococcus spp. strains to streptomycin (100%), novobiocin (100%), tetracycline (100%) and Enterococcus spp. to gentamicin (100%), streptomycin (66.7%) and erythromycin (66.7%). The obtained preliminary results indicate a potential risk that future treatments of sheep mastitis may not be effective, and demonstrated that raw unpasteurized milk can harbor antimicrobial resistance pathogens posing a threat to public health. However, further investigations involving molecular research on resistance genes are required to draw comprehensive conclusions.</p>","PeriodicalId":23690,"journal":{"name":"Veterinary Research Communications","volume":"49 1","pages":"30"},"PeriodicalIF":1.8,"publicationDate":"2024-11-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142688847","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The interrelationship between macrominerals and heat stress in ruminants: current perspectives and future directions - a review.","authors":"Emad M Samara, Mohammed A Al-Badwi, Khalid A Abdoun, Mutassim M Abdelrahman, Aly B Okab, Majdi A Bahadi, Ahmed A Al-Haidary","doi":"10.1007/s11259-024-10597-5","DOIUrl":"10.1007/s11259-024-10597-5","url":null,"abstract":"<p><p>Macromineral imbalances in ruminants, particularly in tropical and subtropical regions, pose a significant challenge to production sustainability and profitability. Heat stress exacerbates these imbalances, negatively impacting physiological functions and productivity. This review examines the effects of heat stress on macromineral levels in ruminants and the need for supplementation under such conditions. Heat stress lowers key macrominerals (Na+, K+, Cl-, Ca + 2, Mg + 2, inorganic P) and disrupts acid-base balance due to thermoregulatory responses and reduced feed intake. Supplementing macrominerals to the diet to achieve higher dietary cation-anion difference helps mitigate heat-related morbidity and maintains ruminant health and productivity. A more practical approach, such as sustained-release macromineral boluses in the rumen, is proposed to provide more consistent benefits. Further researches are warranted to optimize supplementation strategies and fully understand macromineral nutrition for heat-stressed ruminants.</p>","PeriodicalId":23690,"journal":{"name":"Veterinary Research Communications","volume":"49 1","pages":"29"},"PeriodicalIF":1.8,"publicationDate":"2024-11-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142688884","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Efficacy of Lactobacillus acidophilus and yeast cell wall-derived supplements on immunity responses, growth performance, and disease resistance in Cyprinus carpio juveniles.","authors":"Hamed Ghafarifarsani, Saman Ahani, Maryam Aftabgard, Sara Ahani, Morteza Yousefi","doi":"10.1007/s11259-024-10567-x","DOIUrl":"https://doi.org/10.1007/s11259-024-10567-x","url":null,"abstract":"<p><p>This study investigated the effects of probiotic Lactobacillus acidophilus (PTCC 1643) (LA) and a commercial yeast cell wall prebiotic (Immunogen^®) (IM) on immunoantioxidant, growth, and digestive status, and resistance to Aeromonas hydrophila in Cyprinus carpio juveniles. The fish were fed in five treatments including control (T1), LA1.5 (T2): 1.5 (× 10^8 CFU/g of LA)/kg diet, LA3 (T3): 3 (× 10^8 CFU/g of LA)/kg diet, IM1 (T4): 1 g of IM/kg diet, and IM2 (T5): 2 g of IM/ kg diet. After 60 days, the function of growth and intestinal protease and amylase showed a significant increase in IM treatments. Serum levels of total protein and superoxide dismutase in LA3 treatment, as well as albumin, catalase, and glutathione peroxidase in LA3 and IM2 treatments were significantly increased. Serum level of malondialdehyde in LA and IM treatments, as well as alkaline phosphatase (ALP) in LA treatments, alanine aminotransferase in LA and IM2 treatments, and lactate dehydrogenase in LA3 treatment was significantly decreased. Serum levels of all the immune parameters in LA3 treatment, as well as alternative complement pathway hemolytic and nitroblue tetrazolium in IM2 treatment were significantly improved. Mucosal level of protease in LA3 and IM2 treatments, total immunoglobulin, and lysozyme in LA and IM2 treatments, as well as ALP and peroxidase in LA3 treatment was significantly increased. Cumulative mortality rate in LA and IM treatments was significantly decreased compared to control after the 14-day challenge with A. hydrophila. It is recommended to include IM2 in the diet of C. carpio juveniles with regard to its beneficial effects on growth and immunity status.</p>","PeriodicalId":23690,"journal":{"name":"Veterinary Research Communications","volume":"49 1","pages":"23"},"PeriodicalIF":1.8,"publicationDate":"2024-11-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142682925","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Microbiota modulation by the inclusion of Tenebrio molitor larvae as alternative to fermented soy protein concentrate in growing pigs diet.","authors":"Irene Ferri, Matteo Dell'Anno, Alessandro Quiese, Bianca Castiglioni, Paola Cremonesi, Filippo Biscarini, Benedetta Canala, Matteo Santoru, Alessia Colombini, Giancarlo Ruffo, Antonella Baldi, Luciana Rossi","doi":"10.1007/s11259-024-10588-6","DOIUrl":"https://doi.org/10.1007/s11259-024-10588-6","url":null,"abstract":"<p><p>Tenebrio molitor meal represents a promising protein source for animal nutrition due to its low environmental impact and high nutritional value. To date, there is limited data in the literature regarding the effects of Tenebrio molitor meal on the modulation of gut microbiota in growing animals, with most results focusing on poultry rather than pigs. The aim of this study was to evaluate the effects of replacing fermented soy protein concentrate with Tenebrio molitor meal on gut microbiota and feed digestibility in growing pigs. A total of 14 growing pigs (80 ± 2 days old) were randomly allotted to two groups: the control group (CON) was fed a commercial diet containing 4% fermented soy protein concentrate (48% crude protein), and the treatment group (TM) was fed a basal diet containing 5% of T. molitor larvae meal formulated to be isonitrogenous and isoenergetic. The study lasted 28 days. Animals were weekly weighted and feed refuse was routinely measured. Fecal, blood samples, and rectal swabs were collected for analysis. No differences were observed in growth and diet digestibility for the protein and lipid components throughout the trial. No differences in the serum concentrations of albumin, globulin, urea, and interleukin-6 were registered in both groups, suggesting an unaltered health status. The TM group showed a significant difference in the beta diversity index considering the total duration of the trial (treatment effect evaluated with PERMANOVA, R² 0.0771, p value = 0.0099) showing an increased abundance of Elusimicrobium spp. and a decrease in Asteroplasma spp. in TM compared to the CON group (p < 0.05). Obtained findings indicate that 5% T. molitor meal can be included as a partial replacement for soy in growing pig formula without impairing pig growth and gut microbiota composition.</p>","PeriodicalId":23690,"journal":{"name":"Veterinary Research Communications","volume":"49 1","pages":"26"},"PeriodicalIF":1.8,"publicationDate":"2024-11-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142682927","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Novel approach for biomaterial assessment: utilizing the Ex Ovo quail cam assay for biocompatibility pre-screening.","authors":"Zuzana Tirpakova, Zuzana Demcisakova, Lenka Luptakova, Julia Hurnikova, Matus Coma, Lukas Urban, Peter Gal, Lubomir Medvecky, Eva Petrovova","doi":"10.1007/s11259-024-10574-y","DOIUrl":"10.1007/s11259-024-10574-y","url":null,"abstract":"<p><p>In recent years, the chorioallantoic membrane (CAM) has emerged as a crucial component of biocompatibility testing for biomaterials designed for regenerative strategies and tissue engineering applications. This study explores angiogenic potential of an innovative acellular and porous biopolymer scaffold, based on polyhydroxybutyrate and chitosan (PHB/CHIT), using the ex ovo quail CAM assay as an alternative to the conventional chick CAM test. On embryonic day 6 (ED6), we placed the tested biomaterials on the CAM alone or soaked them with various substances, including vascular endothelial growth factor (VEGF-A), saline, or the endogenous angiogenesis inhibitor Angiostatin. After 72 h (ED9), we analyzed blood vessels formation, a sign of ongoing angiogenesis, in the vicinity of the scaffold and within its pores. We employed marker for cell proliferation (PHH3), embryonic endothelium (WGA, SNA), myofibroblasts (α-SMA), and endothelial cells (QH1) for morphological and histochemical analysis. Our findings demonstrated the robust angiogenic potential of the untreated scaffold without additional influence from the angiogenic factor VEGF-A. Furthermore, gene expression analysis revealed an upregulation of pro-angiogenic growth factors, including VEGF-A, ANG-2, and VE-Cadherin after 5 days of implantation, indicative of a pro-angiogenic microenvironment. These results underscore the inherent angiogenic potential of the PHB/CHIT composite. Additionally, monitoring of CAM microvilli growing to the scaffold provides a methodology for investigating the biocompatibility of materials using the ex ovo quail CAM assay as a suitable alternative model compared to the chicken CAM platform. This approach offers a rapid screening method for biomaterials in the field of tissue repair/regeneration and engineering.</p>","PeriodicalId":23690,"journal":{"name":"Veterinary Research Communications","volume":"49 1","pages":"24"},"PeriodicalIF":1.8,"publicationDate":"2024-11-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11582168/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142682948","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}