Lyudmila A Lider, Vladimir Kiyan, Nellya E Mannapova, Irina A Olex, Christian Bauer
{"title":"Seroprevalence of Toxoplasma gondii infection in urban client-owned cats in Kazakhstan.","authors":"Lyudmila A Lider, Vladimir Kiyan, Nellya E Mannapova, Irina A Olex, Christian Bauer","doi":"10.1007/s11259-025-10862-1","DOIUrl":"https://doi.org/10.1007/s11259-025-10862-1","url":null,"abstract":"<p><p>Toxoplasma gondii is the causative agent of one of the most important parasitic zoonoses. Cats and other felids, as definitive hosts, play a central role in the epidemiology of this protozoan parasite. However, current knowledge about the occurrence of toxoplasmosis in cats in the Central Asian countries of the former Soviet Union remains limited. In Kazakhstan, human toxoplasmosis has received little attention from public health authorities. The aim of this study was to gather information on the current seroprevalence of toxoplasmosis in urban cats in the country. Serum samples were collected from 273 client-owned cats during consultations at veterinary clinics in five cities (Almaty, Astana, Oral, Qostanai, and Shymkent) between January and February 2024. Samples were analyzed for T. gondii-specific immunoglobulin G (IgG) antibodies using a commercially available enzyme-linked immunosorbent assay. Differences in seroprevalence among groups of cats based on city, sex, and age class were assessed for statistical significance using the chi-squared test. T. gondii-specific IgG antibodies were detected in 50 (18.3%) of the cats tested. Seroprevalence was not significantly associated with city of origin or sex; however, it increased significantly with age. Although these data should not be considered statistically representative of the national cat population in Kazakhstan due to non-random sampling, they can still serve as a basis for local health authorities to make informed decisions regarding education and prevention measures aimed at reducing the risk of toxoplasmosis in humans, in accordance with the \"One Health\" approach.</p>","PeriodicalId":23690,"journal":{"name":"Veterinary Research Communications","volume":"49 5","pages":"291"},"PeriodicalIF":2.0,"publicationDate":"2025-08-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144970986","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Tishita Sen Ape, Shuvo Singha, Unusing Marma, Hasnat Jahan Rumi, Sirajul Islam Sagor, Antonella Chiariotti, Vittoria Lucia Barile, Ylva Persson, Md Mizanur Rahman
{"title":"Water buffalo farming, udder health and its dairy production status in Bangladesh: Practices, challenges, and potentialities.","authors":"Tishita Sen Ape, Shuvo Singha, Unusing Marma, Hasnat Jahan Rumi, Sirajul Islam Sagor, Antonella Chiariotti, Vittoria Lucia Barile, Ylva Persson, Md Mizanur Rahman","doi":"10.1007/s11259-025-10823-8","DOIUrl":"https://doi.org/10.1007/s11259-025-10823-8","url":null,"abstract":"<p><p>Buffalo farming in Bangladesh plays a key role in enhancing food security by providing a valuable protein source through milk and meat production. Water buffalo are well-adapted to harsh climate conditions due to their robust immune systems, while they may adapt with minimal feed availability compared to dairy cows. Despite its adaptability and importance, buffalo farming has been underprioritized compared to cattle farming due to several challenges, such as a lack of awareness among farmers of modern dairy practices, limited access to good quality feed, poor breeding programs, improper management practices, and inadequate veterinary services. Furthermore, the lack of a dedicated buffalo product chain, fragmented market, and limited policy support have further impeded the development of this sector, leading to reduced profitability. Despite similar climatic conditions, Bangladesh's buffalo population remains underexploited, contributing minimally to global buffalo milk production. In contrast, several neighboring countries, including India, Pakistan, and Nepal, lead global buffalo milk production, highlighting a significant gap in Bangladesh's buffalo sector. Despite these constraints, buffalo farming holds great potential, particularly for producing high-nutrient products such as yogurt and cheese, which are popular in many regions. Recognizing the potential of buffalo farming, the government and other organizations are collaborating on initiatives such as enhanced breeding programs, policy support, and extension services for farmers. This review highlights the challenges and prospects of water buffalo farming, including the need for strategic interventions and key research areas to exploit the potential in dairy enterprises in Bangladesh.</p>","PeriodicalId":23690,"journal":{"name":"Veterinary Research Communications","volume":"49 5","pages":"292"},"PeriodicalIF":2.0,"publicationDate":"2025-08-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12394335/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144971016","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Menthol as a sustainable alternative anaesthetic for adult zebrafish (Danio rerio).","authors":"Luís Félix, Sandra M Monteiro, Carlos Venâncio","doi":"10.1007/s11259-025-10860-3","DOIUrl":"https://doi.org/10.1007/s11259-025-10860-3","url":null,"abstract":"<p><p>The use of anaesthetics has become pivotal in promoting ethical, sustainable, and responsible welfare practices in aquaculture and research procedures. However, species-specific reactions and side-effects have arisen from the use of the most common anaesthetics (MS-222 and eugenol). This study investigated the potential of menthol, a naturally derived and environmentally friendly compound, as an alternative immersion anaesthetic for adult zebrafish (Danio rerio) through concentration-response assays and the evaluation of cardiorespiratory, locomotion and aversive-related effects. Concentrations below 25 mg/L did not induce anaesthetic states while concentrations of 50 and 75 mg/mL were considered the most effective, as they successfully induced anaesthesia with induction and recovery times below 400 s. These values were comparable to those observed with MS-222. In addition, no mortality was observed at these concentrations despite the transient depression of the cardio-respiratory system. The video tracking analyses showed no significant behavioural changes for the lowest concentrations while relatively narrow behavioural safety margins were noticed for the highest concentrations (> 150 mg/L). In addition, concentrations above 75 mg/L induced aversive behaviours which do not support their use for fish anaesthesia. Based on these findings, a short-exposure (10-min) was conducted using menthol concentration of 50 mg/L, resulting in cortisol values equal to those of the control group. Overall, this study shows that a menthol concentration of 50 mg/L can be used as an alternative and more sustainable anaesthetic for zebrafish while higher concentrations may compromise fish welfare.</p>","PeriodicalId":23690,"journal":{"name":"Veterinary Research Communications","volume":"49 5","pages":"288"},"PeriodicalIF":2.0,"publicationDate":"2025-08-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12380966/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144971022","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Preventive effect of matrine on clostridium perfringens type A-induced diarrhoea in piglets.","authors":"Siyi Xing, Mengran Zhang, Dujian Yan, Tian Tian, Aohang Yu, Chenchen Wu","doi":"10.1007/s11259-025-10856-z","DOIUrl":"https://doi.org/10.1007/s11259-025-10856-z","url":null,"abstract":"<p><p>Clostridium perfringens (Cp) type A is one of the primary microorganisms responsible for bacterial diarrhoea in weaning piglets. Matrine, a tetracyclic quinolizidine alkaloid, exhibits diverse bioactive properties, including anti-inflammatory and neuroprotective effects. To better understand the anti-inflammatory mechanisms of Matrine on piglets infected with Cp type A (CpA). We investigated its preventive effects against diarrhoea over a 14-days period. Our results showed that 30 mg/kg of Matrine is non-toxic and does not harm the histological structure of organ tissues or alter the haematological indices of piglets. Matrine significantly reduced the abundance of CpA in faecal and intestines, as well as the content of toxin-α. The inflammatory response was mitigated following Matrine treatment, and physiological organ functions were restored, particularly through the repair of intestinal mucosal layer and sIgA level. Matrine also promoted a high abundance of beneficial intestinal microbiota, including Lactobacillus, Bacteroidota, Muribaculaceae, and Oscillospirales. This microbial composition inhibited the abundance of epithelial cells by Cp, thereby further reducing the diarrhoea rate and mortality in piglets. In conclusion, Matrine promotes the abundance of intestinal probiotics, inhibits the colonization of CpA, reduces the inflammatory response as well as restores intestinal tissue hismorphology. It will be used as a therapeutic agent diarrhea of piglets infected with CpA in the furture.</p>","PeriodicalId":23690,"journal":{"name":"Veterinary Research Communications","volume":"49 5","pages":"289"},"PeriodicalIF":2.0,"publicationDate":"2025-08-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144971011","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Piotr Trębacz, Jan Frymus, Michał Czopowicz, Mateusz Pawlik, Anna Barteczko, Aleksandra Kurkowska
{"title":"Nerve palpation to reduce the risk of ulnar nerve injury associated with caudo-medial arthroscopic portal creation in the canine elbow - a cadaveric study.","authors":"Piotr Trębacz, Jan Frymus, Michał Czopowicz, Mateusz Pawlik, Anna Barteczko, Aleksandra Kurkowska","doi":"10.1007/s11259-025-10859-w","DOIUrl":"https://doi.org/10.1007/s11259-025-10859-w","url":null,"abstract":"<p><p>The medial portal is the most common type of optical portal used in canine elbow arthroscopy. The caudo-medial arthroscopic portal can be used to improve visualization of humeral condylar lesions and medial coronoid process pathologies, as well as for more accurate identification of the humeral intercondylar fissure. However, due to the proximity of the nerve, caution must be exercised when creating this portal. This study investigated whether the risk of ulnar nerve injury associated with caudo-medial arthroscopic portal placement in canine cadavers could be reduced by palpating the ulnar nerve before creating the portal for the arthroscope, as is done in human elbow arthroscopy. Twenty-six canine cadavers were enrolled in the study: 15 males and 11 females, weighing between 12 and 41 kg. In the 26 dogs (52 elbows) in which ulnar nerve palpation was performed, the distance between the nerve and the arthroscopic sheath ranged from 0 to 10.8 mm. Overall, ulnar nerve injury was observed in 4 / 26 dogs (15%, CI 95%: 6%, 34%): in the left elbow of one dog and in the right elbows of three dogs. Comparison with previous studies indicated that implementing palpation of the ulnar nerve before creating the portal for the arthroscope reduced the risk of ulnar nerve injury threefold, from approximately 50% to approximately 15%. Conclusions: Following palpation and identification, the incidence of ulnar nerve injury is low.</p>","PeriodicalId":23690,"journal":{"name":"Veterinary Research Communications","volume":"49 5","pages":"287"},"PeriodicalIF":2.0,"publicationDate":"2025-08-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12378507/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144970976","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Natalia Carrasco, Romina Levit, Cecilia Aristimuño Ficoseco, Alejandra de Moreno de LeBlanc, Jean Guy LeBlanc, María Elena Fátima Nader-Macías
{"title":"Isolation, identification and selection of beneficial canine homologous lactic acid bacteria by their potential probiotic characteristics.","authors":"Natalia Carrasco, Romina Levit, Cecilia Aristimuño Ficoseco, Alejandra de Moreno de LeBlanc, Jean Guy LeBlanc, María Elena Fátima Nader-Macías","doi":"10.1007/s11259-025-10851-4","DOIUrl":"https://doi.org/10.1007/s11259-025-10851-4","url":null,"abstract":"<p><p>Companion animals have become an increasingly important part of human life, consequently, there is a significant increase in the demand of natural food ingredients and functional foods in order to improve their health and well-being. Probiotics have been used in humans and production animals and have recently attracted the focus of the pet industry. Therefore, the main objective of this work was to isolate, and survey the different beneficial properties of lactic acid bacteria (LAB) strains from dogs, in order to advance in the design of probiotic formula that would be beneficial for canine health. One hundred presumptive LAB isolated from mother's milk and faeces of canine puppies and adult dogs were selected and their surface, enzymatic activities and adhesion properties were studied: 40% showed high or medium hydrophobicity, 13% self-aggregation, 20% cellulase activity and 19% feruloyl esterase activity. Based on the results, 60 LAB were pre-selected and their biofilm formation was evaluated in media without Tween 80, and their EPS production on MRS with glucose, sucrose or lactose. Also, 60% of the isolates grew on folate-free medium, while 53% on medium lacking riboflavin. The genetic identification of 30 isolates indicated: 4 L. johnsonii, 2 L. salivarius, 2 L. plantarum, 2 S. thoraltensis, 2 P. acidilactici, 7 E. canintestini, 4 E. faecalis, 6 E. faecium and 1 E. durans. Finally, ten of the selected LAB strains evidenced adhesion ability to eucaryotic cells close to 70%. This work supports the selection of LAB strains with the best characteristics through laboratory assays, and to define those sharing properties, in a way to define their optimal combinations, to further advance in the design of probiotic formulas containing homologous strains for the health of canines.</p>","PeriodicalId":23690,"journal":{"name":"Veterinary Research Communications","volume":"49 5","pages":"285"},"PeriodicalIF":2.0,"publicationDate":"2025-08-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144971247","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Xin Wang, Lijuan Kang, Yongni Guo, Yilong Jiang, Zelong Wang, Bo Kang
{"title":"Effects of spermidine-rich diet on follicle development and steroidogenesis in Sichuan white geese.","authors":"Xin Wang, Lijuan Kang, Yongni Guo, Yilong Jiang, Zelong Wang, Bo Kang","doi":"10.1007/s11259-025-10845-2","DOIUrl":"https://doi.org/10.1007/s11259-025-10845-2","url":null,"abstract":"<p><p>Polyamines have been reported to play a role in the regulation of follicular development and steroidogenesis, the underlying mechanisms remain unclear. This study examined the effects of spermidine-enriched diets on ovarian follicle development and steroid hormone synthesis in Sichuan white geese. Forty healthy female Sichuan white geese were randomly assigned to five groups(Con, 5SPG, 10SPG, 5SPD, and 10SPD). The Con group were provided with basal diet, while the 5SPG and 10SPG groups were fed wheat germ feed containing 5 mg/kg or 10 mg/kg spermidine, respectively. Similarly, the 5SPD and 10SPD groups received basal diets containing 5 mg/kg and 10 mg/kg pure spermidine, respectively. In the 5SPG group, ovarian spermidine and putrescine concentrations, along with serum and follicular E2 and P4 levels, increased significantly (P < 0.05). Serum malondialdehyde (MDA) level decreased, whereas BCL-2 gene expression was significantly elevated compared with the control group (P < 0.05). Furthermore, the expression of StAR and 3β-HSD in yellow follicles and of 17β-HSD and CYP17A1 in white follicles was significantly upregulated in the 5SPG group (P < 0.05). Conversely, the 10SPD group exhibited severe follicular damage, increased numbers of white follicles (P < 0.05), and reduced polyamine and E2 concentrations in prehierarchical follicles (P < 0.05). Additionally, the expression of steroidogenesis-related genes was significantly reduced (P < 0.05), whereas ovarian MDA concentrations and Caspase-3 and Bax expression were significantly upregulated (P < 0.05). Collectively, these results indicate that a wheat germ diet containing 5 mg/kg spermidine enhanced ovarian antioxidant capacity, modulated steroid hormone levels through the regulation of steroidogenesis-related gene expression, and promoted follicular development in Sichuan white geese.</p>","PeriodicalId":23690,"journal":{"name":"Veterinary Research Communications","volume":"49 5","pages":"286"},"PeriodicalIF":2.0,"publicationDate":"2025-08-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144971270","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Juan Agustín García, Alejandro Rodríguez, María Valeria Scioli, Ignacio Azaldegui, Agustín Tiberio, Martina Pachiani, Elvis Vilatuña, Emiliano Sosa, Germán José Cantón
{"title":"Potential reproductive impairment due to Solanum glaucophyllum-induced calcinosis in female ruminants.","authors":"Juan Agustín García, Alejandro Rodríguez, María Valeria Scioli, Ignacio Azaldegui, Agustín Tiberio, Martina Pachiani, Elvis Vilatuña, Emiliano Sosa, Germán José Cantón","doi":"10.1007/s11259-025-10857-y","DOIUrl":"https://doi.org/10.1007/s11259-025-10857-y","url":null,"abstract":"<p><p>Solanum glaucophyllum is a toxic calcinogenic plant endemic to Central Argentina and is widely recognized as the primary cause of enzootic calcinosis in ruminants. Although the systemic calcification induced by this plant is well documented, its potential impact on reproductive performance remains underexplored. The present study reports the first evidence of calcinosis affecting the female reproductive organs of ruminants. Reproductive tissues-including the uterus, ovary, and oviduct-were obtained from a non-pregnant 4-year-old Texel ewe and a 6-year-old Hereford cow that exhibited gross signs of systemic calcification following S. glaucophyllum ingestion. Histopathological examination revealed extensive granular basophilic calcium deposits within the tunica media and/or intima of blood vessels in these organs, accompanied by varying degrees of non-mineralized hyperplasia. The deposits were confirmed as calcium by Von Kossa staining. These findings suggest that vascular calcification in reproductive tissues may reduce blood flow, thereby impairing the delivery of hormones essential for follicular growth, ovulation, fertilization, and embryo implantation. Given that subclinical calcinosis may compromise reproductive efficiency, this phenomenon could have significant economic repercussions for the livestock industry. Further investigations are warranted to quantitatively assess the degree of calcification, vascular perfusion, and additional reproductive parameters to elucidate the impact of S. glaucophyllum-induced calcinosis on fertility.</p>","PeriodicalId":23690,"journal":{"name":"Veterinary Research Communications","volume":"49 5","pages":"284"},"PeriodicalIF":2.0,"publicationDate":"2025-08-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144970991","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Andrés Fraile-Fernandez, Manuel Fuertes-Recuero, Jorge Espinel-Rupérez, Alejandro Cárdenas-Osuna, Ricardo Ruano Barreda, Javier Horcajada-Garcia, Gustavo Ortiz-Diez
{"title":"Correction: Reconstruction of a rostral maxillary defect by two custom-made titanium implants following a partial maxillectomy for treatment of squamous cell carcinoma in a dog.","authors":"Andrés Fraile-Fernandez, Manuel Fuertes-Recuero, Jorge Espinel-Rupérez, Alejandro Cárdenas-Osuna, Ricardo Ruano Barreda, Javier Horcajada-Garcia, Gustavo Ortiz-Diez","doi":"10.1007/s11259-025-10863-0","DOIUrl":"https://doi.org/10.1007/s11259-025-10863-0","url":null,"abstract":"","PeriodicalId":23690,"journal":{"name":"Veterinary Research Communications","volume":"49 5","pages":"283"},"PeriodicalIF":2.0,"publicationDate":"2025-08-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12373692/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144971321","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Tong Liu, Huasong Bai, Hengyan Wang, Yunliang Li, Zhanzhong Wang
{"title":"Lysozyme and dandelion extract alleviate tearing eye disease by reducing inflammation and oxidative stress through canine corneal fibroblast model established.","authors":"Tong Liu, Huasong Bai, Hengyan Wang, Yunliang Li, Zhanzhong Wang","doi":"10.1007/s11259-025-10846-1","DOIUrl":"10.1007/s11259-025-10846-1","url":null,"abstract":"<p><p>Tearing around the eyes was a common health problem in dogs and cats. The alleviating effects of lysozyme and dandelion extract on inflammatory reaction of canine corneal fibroblasts induced by lipopolysaccharide (LPS) were systematically investigated. Based on inflammatory model established, inflammatory factors (IL-6, IL-8, IL-10, TNF-α) and oxidative stress indicators (SOD, CAT, GSH, MDA) were utilized to evaluate inflammatory response. The anti-inflammatory mechanisms of the two substances were also explored by transcriptome sequencing. The results demonstrated that lysozyme and dandelion extract at a safe concentration (1-10 µg/mL) could significantly reduce the release of nitric oxide by 59%-72% (P < 0.05), increase the concentrations of SOD, CAT and GSH by 2-9 times (P < 0.05), and decrease the concentration of MDA by 35%-61% (P < 0.05). The concentrations of IL-6, IL-8 and TNF-α decreased by 10%-44% (P < 0.05), while the concentrations of IL-10 and LXA-4 increased by 21%-39% (P < 0.05). The number of migrating cells increased by 42%-56% (P < 0.05). Therefore, the two substances alleviated oxidative damage, inhibited the production of inflammatory factors and improved cell migration ability. Transcription GO enrichment results confirmed that lysozyme and dandelion extracts could up-regulate the gene expression of \"structural molecule activity\" and \"ribosome\" terms. The results of KEGG enrichment showed that lysozyme affected \"ribosome\" and other pathways, while dandelion extract participated in \"axon guidance\", \"amoebiasis\", \"ECM-receptor interaction\", \"focal adhesion\" pathways, which meant that lysozyme and dandelion extract exert crucial function in alleviating corneal cell inflammation by affecting the expression of genes or enzymes in these pathways. These findings could provide significant insights into reducing tearing eye inflammation in canine and strategies for preventing or removing tears and tear residue with lysozyme and dandelion extract.</p>","PeriodicalId":23690,"journal":{"name":"Veterinary Research Communications","volume":"49 5","pages":"282"},"PeriodicalIF":2.0,"publicationDate":"2025-08-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144875457","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}