Veterinary Research Communications最新文献

{"title":"Dynamic changes in plasma electrophoretic protein profiles of different goat breeds during peripartum: implications for immuno-inflammatory monitoring.","authors":"Stella Agradi, Alda Quattrone, Giulio Curone, Gabriele Brecchia, Federica Arrigo, Alessandro Zumbo, Giuseppe Piccione, Maria Rizzo, Francesca Arfuso","doi":"10.1007/s11259-025-10896-5","DOIUrl":"https://doi.org/10.1007/s11259-025-10896-5","url":null,"abstract":"<p><p>The peripartum period is marked by profound physiological adjustments related to fetal development and the onset of lactation, which can affect homeostasis and alter plasma protein profiles. This study aimed to evaluate and compare changes in plasma protein fractions in healthy goats from four Italian breeds: Camosciata delle Alpi (n = 15), Orobica di Valgerola (n = 20), Frisa Valtellinese (n = 22), and Lariana (n = 19) during this transitional phase. Blood samples were collected at five time points: thirty days before parturition (T1), fifteen days before parturition (T2), at parturition (T3), fifteen days after parturition (T4), and thirty days after parturition (T5). All breeds exhibited dynamic variations in total protein levels and electrophoretic fractions throughout the peripartum period. Frisa and Lariana breeds showed a post-partum increase in α- and β-globulin fractions, suggesting a potentially greater inflammatory response compared to the other breeds. These findings highlight the metabolic demands of the peripartum period and the breed-specific adaptations involved. Plasma protein electrophoresis proved to be an effective tool for monitoring physiological changes and may support the early detection of metabolic imbalances. The study also underscores the importance of developing breed-specific reference intervals to enhance health assessment and herd management in goats.</p>","PeriodicalId":23690,"journal":{"name":"Veterinary Research Communications","volume":"49 6","pages":"351"},"PeriodicalIF":2.0,"publicationDate":"2025-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145252926","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"High genetic diversity of Ehrlichia canis trp36 and p28 genes in dogs from Rio de Janeiro, Brazil.","authors":"Jônathan David Ribas Chagas, Diogo Santos Maia, Matheus Dias Cordeiro, Zbigniew Zając, Angélique Foucault-Simonin, Alejandro Cabezas-Cruz, Huarrisson Azevedo Santos, Douglas McIntosh, Claudia Bezerra da Silva, Bruna de Azevedo Baêta","doi":"10.1007/s11259-025-10934-2","DOIUrl":"https://doi.org/10.1007/s11259-025-10934-2","url":null,"abstract":"<p><p>Ehrlichia canis is an obligate intracellular bacterium and the etiologic agent of canine monocytic ehrlichiosis, an infectious disease that affects dogs and is often associated with thrombocytopenia. This study aimed to analyze the genetic variability of E. canis in thrombocytopenic dogs from two mesoregions in the state of Rio de Janeiro, Brazil. We analyzed 404 blood samples from dogs with thrombocytopenia using the dsb, p28, and trp36 genes as molecular targets. The positivity rate was 21.04% (85/404) for the dsb and p28 genes, while the trp36 gene had a detection rate of 34.1% (29/85) in the positive samples. To analyze genetic diversity, 23 samples positive for the p28 gene and 29 samples based on trp36 results were sequenced. Phylogenetic analysis revealed high genetic diversity among the circulating E. canis strains in the mesoregions studied, with the identification of distinct genogroups, including the American and Brazilian genogroups. Analysis of the p28 gene revealed 17 distinct haplotypes (Hd = 0.9872) with region-specific clustering. The haplotype network mirrored the trp36-defined genogroups, suggesting genetic structuring associated with the American and Brazilian variants. It is concluded that there is high genetic diversity in the trp36 and p28 genes of E. canis strains circulating in dogs in the Metropolitan and south Fluminense mesoregions of Rio de Janeiro state, highlighting the importance of molecular surveillance to better understand the epidemiology of canine ehrlichiosis and the strains involved in the infection.</p>","PeriodicalId":23690,"journal":{"name":"Veterinary Research Communications","volume":"49 6","pages":"350"},"PeriodicalIF":2.0,"publicationDate":"2025-10-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145252979","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Therapeutic effects of adipose mesenchymal stem cell-derived exosomes on limbal stem cells deficiency in cats.","authors":"Irem Ergin, Sumeyye Sainkaplan, Melis Isik, Ozge Ozgenc Cinar, Alev Gurol Bayraktaroglu, Burak Derkus, Oytun Okan Senel","doi":"10.1007/s11259-025-10908-4","DOIUrl":"https://doi.org/10.1007/s11259-025-10908-4","url":null,"abstract":"<p><p>Corneal transparency is maintained by the limbus and its stem cells, and damage or deficiency in this region leads to conjunctivalization and subsequent vision impairment. This study aims to evaluate the therapeutic effects of feline adipose mesenchymal stem cell-derived exosomes (fAD-MSCExo) in the treatment of limbal stem cell deficiency in cats. For this purpose, 30 animals presenting with severe conjunctivalization were included in the study. Cats were randomly assigned to two groups: Group 1 (n = 15), serving as the control group and receiving steroid treatment, Group 2 (n = 15), receiving fAD-MSCExo therapy. Exosomes were produced from fAD-MSCs isolated from intra-abdominal adipose tissue obtained from three healthy donor cats. Detailed ophthalmologic examination and impression cytology were performed on all animals preoperatively and on postoperative days 0, 10, and 30. Periodic acid-schiff staining was used to detect goblet cells, while Immunofluorescence staining was performed to identify K13 cytokeratin antibodies. At postoperative day 10, no significant clinical or histological differences were observed between the two groups. However, after treatment discontinuation, by postoperative day 30, clinical improvement had regressed in the steroid-treated group, whereas corneal epithelial regeneration and, consequently, limbal repair had progressed satisfactorily in the exosome-treated group. This study demonstrated that even short-term use of exosomes is effective in the treatment of limbal stem cell deficiency in cats. The presented data suggest that fAD-MSCExo may offer a potential definitive solution for the management of this widespread pathology, which requires long-term follow-up, thereby generating significant interest in further research in this field.</p>","PeriodicalId":23690,"journal":{"name":"Veterinary Research Communications","volume":"49 6","pages":"349"},"PeriodicalIF":2.0,"publicationDate":"2025-10-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145239895","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ultrasound-guided serratus plane injection using superficial and deep approaches in cat cadavers: anatomical and dye spread study.","authors":"Marina Moresco, Marco Aurélio Camargo-Fontanela, Gilberto Serighelli-Júnior, Fabiano Montiani-Ferreira, Marcello Machado, Juan Carlos Duque-Moreno","doi":"10.1007/s11259-025-10923-5","DOIUrl":"https://doi.org/10.1007/s11259-025-10923-5","url":null,"abstract":"<p><p>This study aims to describe the anatomy and sonoanatomy of the lateral thoracic wall of cat cadavers and compare the spread of a bupivacaine-dye solution into the superficial (SSP) or deep (DSP) planes of the serratus ventralis thoracis (SVT) muscle. One formalin-fixed and sixteen frozen-thawed feline cadavers (32 hemithoraces) were used and randomly assigned to receive either a SSP or DSP ultrasound-guided injection. With cadavers in lateral recumbency, a linear transducer was transversely placed between the fourth and fifth ribs, at the level of the scapulohumeral joint and a 22-gauge Tuohy needle was inserted in-plane, in a caudocranial direction, until the tip was in the desired fascial plane. Bupivacaine-dye solution (0.3 mL/kg) was injected. Injectate placement and number of stained nerves were assessed by dissection. Differences were considered when p ≤ 0.05. The intercostobrachial nerve (nIC), lateral cutaneous branches of intercostal nerves 3 to 9 in SSP, and lateral cutaneous branches of intercostal nerves 3 to 7 in DSP were identified. Injection success was 93.75% (SSP) and 100% (DSP). In the SSP group, the nIC and the lateral cutaneous branches of intercostal nerves 3 to 7 were stained in 26.6; 66.6; 93.3; 86.6; 26.6 and 6.6% of cases and 31.2; 68.7; 87.5; 75.0; 56.2 and 18.7% in the DSP group, respectively. The sonoanatomy and landmarks for SSP and DSP plane injections have been successfully described in cats. Both approaches stained nerves involved in thoracic wall sensory innervation and appear feasible for regional analgesia. Clinical studies are warranted to assess their efficacy.</p>","PeriodicalId":23690,"journal":{"name":"Veterinary Research Communications","volume":"49 6","pages":"348"},"PeriodicalIF":2.0,"publicationDate":"2025-10-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145239831","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular characterization of Rhipicephalus microplus and tick-borne pathogens in cattle in Nepal.","authors":"Gita Sadaula Pandey, Chet Raj Pathak, Amir Sadaula, Prajwol Manandhar, Rabin Bastakoti, Mackenzie L Kwak, Naoki Hayashi, Nariaki Nonaka, Ryo Nakao","doi":"10.1007/s11259-025-10913-7","DOIUrl":"https://doi.org/10.1007/s11259-025-10913-7","url":null,"abstract":"<p><p>Ticks and tick-borne pathogens (TBPs) have a significant economic impact on livestock. In Nepal, only limited information is available for the molecular identification of ticks and TBPs. This study aims to address this major challenge by providing data on the diversity of TBPs in Nepalese cattle, along with molecular sequences to facilitate accurate identification. A total of 145 cattle blood samples were collected from Pokhara (n = 70) and Madi (n = 75). The cattle were also examined for tick infestations, and the detected ticks were subjected to morphological identification, followed by molecular characterization using the cytochrome c oxidase subunit I (COI) gene. The heat shock protein (groEL) and citrate synthase (gltA) genes were targeted for screening Anaplasmataceae. Piroplasmida infections were initially screened using nested polymerase chain reaction (PCR) targeting the 18S ribosomal RNA gene. Theileria orientalis and Anaplasma marginale were further characterized by the major surface protein 4 (MSP4) gene. Rhipicephalus microplus was the only tick species detected in the examined cattle. Overall, 29.7% (43/145) of the cattle examined were infected with at least one TBP, with 3.4% (5/145) being co-infected with more than one TBP. The detection rate of TBPs was 20% (n = 29) for A. marginale, 3.4% (n = 5) for Anaplasma bovis, 6.2% (n = 9) for T. orientalis, and 4.1% (n = 6) for Theileria annulata. The phylogenetic analysis showed that two genotypes of A. marginale and T. orientalis (types 5 and 7) were present in cattle. This study provides preliminary baseline data on the prevalence and genetic diversity of ticks and TBPs in cattle in Nepal.</p>","PeriodicalId":23690,"journal":{"name":"Veterinary Research Communications","volume":"49 6","pages":"346"},"PeriodicalIF":2.0,"publicationDate":"2025-10-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145226204","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"First serological evidence of equine coronavirus and severe acute respiratory syndrome coronavirus 2 in horses in North Africa.","authors":"Walid Kouadria, Sophie Le Poder, Kees van Maanen, Torsten Seuberlich, Kara L D Dawson, Stéphan Zientara, Farouk Laabassi","doi":"10.1007/s11259-025-10928-0","DOIUrl":"https://doi.org/10.1007/s11259-025-10928-0","url":null,"abstract":"<p><p>Viral diseases cause significant economic losses within the equine population. Horses are susceptible to equine coronavirus (ECoV) and severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2), although only ECoV has been associated to clinical disease. The aim of this study was to investigate, for the first time in Algeria, the seroprevalence of ECoV and SARS-CoV-2 antibodies and the prevalence of ECoV infection in horses. In 2022, a total of 299 serum samples was collected from horses aged 1 to 27 years. Serological analysis for the presence of ECoV and SARS-CoV-2 was performed using a validated in-house and a commercially available ELISA, respectively. In addition, fecal samples of these animals were tested for the presence of ECoV RNA by RT-qPCR. SARS-CoV-2-ELISA positive sera with high S/P ratios and negative samples close to the doubtful threshold were retested using a virus neutralization test (VNT). The seroprevalence of ECoV and SARS-CoV-2 in the tested horses was 63.5% (190/299) and 4.3% (13/299), respectively. Among CoVs-seropositive horses, six were seropositive for both ECoV and SARS-CoV-2, thus 6/10 sera were VNT positive, including two ELISA-negative samples for SARS-CoV-2. ECoV seroprevalence varied according to age, breed and sex. None of the fecal samples tested positive for ECoV. Antibodies against SARS-CoV-2 were confirmed by VNT in six samples (2%). One SARS-CoV-2-positive serum tested by ELISA and confirmed through VNT was cytotoxic for VERO cells. This study is the first to report the circulation of ECoV and SARS-CoV-2 in the Algerian horse population. Further studies are necessary to isolate and obtain molecular characterisation of ECoV and SARS-CoV-2 from horses in Algeria.</p>","PeriodicalId":23690,"journal":{"name":"Veterinary Research Communications","volume":"49 6","pages":"347"},"PeriodicalIF":2.0,"publicationDate":"2025-10-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145226219","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antibacterial, anti-biofilm and anti-virulence activity of biosynthesized silver nanoparticles against drug-resistant Staphylococcus aureus.","authors":"Jaideep Singh, Ravi Kant Agrawal, Kajal Bankoti, Richa Sarkar, Mrinalini Saini, Keshav Kashyap, Deepak Kumar, Gaurav Kumar Sharma, Prabhat Nath Jha, Sonali Jain, Bhoj Raj Singh","doi":"10.1007/s11259-025-10900-y","DOIUrl":"https://doi.org/10.1007/s11259-025-10900-y","url":null,"abstract":"&lt;p&gt;&lt;p&gt;Antibiotic resistance in bacteria has become a major concern for the effective treatment of infections; therefore, alternatives to antibiotics are being extensively researched to combat drug-resistant microbes. In this study, silver nanoparticles (AgNPs) were biosynthesized using aqueous extracts of papaya leaves (Carica papaya), cannabis leaves (Cannabis sativa), and cardamom (Elettaria cardamomum) and characterized by field-emission scanning electron microscopy (FE-SEM) and UV-visible spectrophotometry. Biosynthesized AgNPs were evaluated for their antibacterial, anti-biofilm, and anti-virulence potential by phenotypic and genotypic methods. AgNPs biosynthesized by all three extracts had spherical morphology and sizes in the nanoscale, average diameter ranging from 46.05 to 94.12 nm. Antibacterial susceptibility testing of S. aureus field isolates under study revealed 48% (24/50) and 38% (19/50) to be resistant to methicillin and amoxycillin-clavulanic acid, respectively. Antibacterial activity of biosynthesized AgNPs against S. aureus strains was determined by the well diffusion method. AgNPs were found to be effective on 90.90% (50/55) S. aureus strains with a zone of inhibition varying from 10 to 21 mm. The AgNPs were also found to be effective on other important bacterial pathogens (viz. Bacillus cereus ATCC 10876, Pseudomonas aeruginosa ATCC 27853, Salmonella Enteritidis ATCC 13070, Escherichia coli ATCC 43888, and Listeria monocytogenes MTCC 657) screened in the study with a ZOI of 15-18 mm. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of AgNPs against S. aureus ranged between 0.015625-0.125 mg/mL and 0.015625-0.25 mg/mL, respectively. In the time kill assay, AgNPs were able to kill S. aureus rapidly within 0.5-1.0 h. In the haemolytic assay, 4-9% haemolysis was observed at concentrations ranging from 0.015625 to 0.25 mg/mL of AgNPs. Biofilm-forming ability of all strains of S. aureus (n = 55) determined by crystal violet assay revealed that 87.27% (48/55) were biofilm formers, while 12.73% (7/55) were non-biofilm formers. Out of 48 biofilm-forming strains, 81.25% (39/48) were strong biofilm producers, 10.41% (5/48) were moderate biofilm producers, and 8.33% (4/48) were weak biofilm producers. Anti-biofilm effect of AgNPs was found at sub-MIC (0.03125 mg/mL), phenotypically. Exopolysaccharide production was found to be reduced by 53.38% indicating the anti-virulence potential of AgNPs at sub-MIC. Relative expression analysis revealed that AgNPs downregulated the expression of biofilm-related genes, namely icaC, icaD, and spa, by 14.2, 10.6, and 8.7-fold, respectively, compared to the control at 3 h of incubation. Other biofilm-related and virulence genes, including icaA, icaB, icaR, agr, ebps, fnb-B, sar-A, and katA, were also found to be downregulated by 7.4, 7.5, 6.2, 5, 4.2, 7.3, 4, and 3.6-fold, respectively, at 3 h. All the target genes were also found to be downregulated at 24 h post-","PeriodicalId":23690,"journal":{"name":"Veterinary Research Communications","volume":"49 6","pages":"345"},"PeriodicalIF":2.0,"publicationDate":"2025-10-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145213687","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"First report of visceral Leishmaniasis in abandoned dogs of the urban area in the central region of Colombia.","authors":"Tuane Ferreira Melo, Josiane Aparecida Martiniano de Pádua, Ana Carolina Amado-Gomes, Natalie Hell-Mor, Fernando Borda Rojas, Luz Ángela Vega, Jose Vallejo Forero, Ricardo Toshio Fujiwara, Ana Paula Peconick, Gabriel Andres Tafur-Gómez","doi":"10.1007/s11259-025-10914-6","DOIUrl":"https://doi.org/10.1007/s11259-025-10914-6","url":null,"abstract":"<p><p>Leishmaniasis is a significant zoonosis, with dogs serving as reservoirs and sand flies as vectors. This survey aimed to identify, for the first time, visceral Leishmaniasis (VL) in dogs in an urban shelter in the Andean region of Colombia. We sampled dogs at four longitudinal time points. We used the rKDDR-plus immunochromatographic test (ICT-rKDDR-plus) and the rKDDR-plus enzyme-linked immunosorbent assay (ELISA-rKDDR-plus) for serological diagnosis. For PCR diagnosis, we used a real-time reaction to amplify the GAPDH gene, and a conventional reaction to amplify the RV1-RV2 genes to identify the Leishmania genus. We also used multiplex PCRs to amplify five genes from Leishmania species for genotyping. Seropositivity in both tests showed 25.3% and 37.5% in dogs from the first and second collections, respectively. These animals also presented clinical and laboratory findings consistent with visceral Leishmaniasis (VL). Following GAPDH gene RT-PCR, we confirmed the amplification of 20 samples, and two samples tested positive for Leishmania spp. using the RV1-RV2 PCR method, the same samples tested positive for L. infantum using multiplex genotyping PCR. The product of the RV1-RV2 PCR exhibited 99.0% similarity to L. infantum following sequencing. These results suggest the presence of VL diagnoses, as determined by serology and PCR methods, in urban areas of the Andean region of Colombia.</p>","PeriodicalId":23690,"journal":{"name":"Veterinary Research Communications","volume":"49 6","pages":"344"},"PeriodicalIF":2.0,"publicationDate":"2025-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145201731","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Short communication: Pharmacological semen collection in dogs via urethral catheterization: evaluation of cardiorespiratory stability and semen quality under continuous dexmedetomidine infusion.","authors":"Jeandson da Silva Carneiro, Guilherme Vitor Freire Guirau, Bruno Henrique da Silva Nogueira, Elaine Dione Venega da Conceição, Tathiana Ferguson Motheo","doi":"10.1007/s11259-025-10907-5","DOIUrl":"https://doi.org/10.1007/s11259-025-10907-5","url":null,"abstract":"<p><p>Pharmacological semen collection (PSC) via urethral catheterization employs α2-adrenergic agonists to induce semen emission. While dexmedetomidine is effective for PSC at doses of 15-20 µg/kg, doses above 3 µg/kg in dogs are associated with notable cardiorespiratory adverse effects. This study evaluated the safety and efficacy of a low-dose sedative protocol for PSC in domestic dogs. Ten male dogs were sedated with intramuscular dexmedetomidine (3 µg/kg) combined with ketamine (3 mg/kg). After 10 min, a continuous dexmedetomidine infusion (3 µg/kg/h) was initiated, and following 10 min of infusion, a propofol bolus (1-2 mg/kg) was administered to allow catheterization and semen collection. Heart rate, respiratory rate, and mean arterial pressure were continuously monitored. Semen samples were analyzed for macroscopic characteristics (color, volume, pH) and microscopic parameters (sperm morphology, plasma membrane integrity). Sperm concentration, and kinectics were assessed using a portable computer-assisted sperm analysis system. The protocol maintained stable cardiopulmonary parameters in all animals. Two dogs did not emit semen; however, the remaining eight yielded samples with a mean volume of 15.49 ± 2.98 µL and a sperm concentration of 693.69 ± 116.15 million spermatozoa/mL. Motility parameters included total motility (20.74 ± 5.17%), progressive motility (12.80 ± 4.32%), curvilinear velocity (51.79 ± 4.04 μm/s), and sperm linearity (22.00 ± 1.68%). The mean proportion of sperm with intact plasma membranes was 53.16 ± 2.52%. In conclusion, the proposed protocol was safe and moderately effective for PSC in dogs; however, the suboptimal semen quality underscores the need for further refinement.</p>","PeriodicalId":23690,"journal":{"name":"Veterinary Research Communications","volume":"49 6","pages":"340"},"PeriodicalIF":2.0,"publicationDate":"2025-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145201796","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mycoplasma leachii strain PG50 shows reduced adherence and invasion compared to M. bovis strains 428E and PG45.","authors":"Daniel W Nielsen, Samantha J Hau, David J Holthausen, Rohana P Dassanayake, Bryan S Kaplan","doi":"10.1007/s11259-025-10901-x","DOIUrl":"10.1007/s11259-025-10901-x","url":null,"abstract":"<p><p>Mycoplasma leachii is a member of the \"Mycoplasma mycoides cluster\", which includes the causative agent of contagious bovine pleuropneumonia (Mycoplasma mycoides subsp. mycoides). Mycoplasma leachii has been associated with severe outbreaks of polyarthritis, mastitis, and abortion in dairy cattle in Australia, Argentina, and China. As M. leachii has veterinary implications and is related to other foreign animal diseases of economic importance, we sought to investigate differences in adherence to and invasion of different bovine cells between M. leachii type strain PG50 and M. bovis strains (type strain PG45 and field strain 428E). Specifically, we tested the strains with Madin-Darby bovine kidney (MDBK), bovine turbinate (BTu), and the carticular progenitor 5 (CP5) cells for 2 and 24 h. Additionally, we tested the MDBK and BTu cells with both non-cytopathic bovine viral diarrhea virus (BVDV-1b) positive and BVDV negative fetal bovine serum. Mycoplasma leachii strain PG50 adhered and invaded less efficiently than M. bovis strains 428E and PG45 with a log₁₀ reduction in adherence and invasion ranging from 1.5 to 2.9 CFU/mL. The reduced adherence and invasion of M. leachii type strain PG50 was observed regardless of the incubation time (2 h vs. 24 h), tested cells (MDBK, BTu, or CP5), cell type (epithelial or fibroblast), or presence of BVDV. Future work should further characterize M. leachii to further assess its risk to animal health and compare it phenotypically to other members of the M. mycoides cluster.</p>","PeriodicalId":23690,"journal":{"name":"Veterinary Research Communications","volume":"49 6","pages":"339"},"PeriodicalIF":2.0,"publicationDate":"2025-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12484092/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145201753","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}