Veterinary Research Communications最新文献

{"title":"Cerebrospinal fluid estradiol fluctuations during the estrous cycle and their association with intraocular pressure and optic nerve sheath diameter in dogs: a first report.","authors":"Candemir Özcan, Serkan Bozacı, Berrak Işık Soytürk, Kenan Çağrı Tümer, Ömer Deniz, Tarık Şafak, Ayşe Başak Dellalbaşı, Özgür Kaynar, Elif Dogan","doi":"10.1007/s11259-025-10820-x","DOIUrl":"https://doi.org/10.1007/s11259-025-10820-x","url":null,"abstract":"<p><strong>Objective: </strong>The relationship between optic nerve sheath diameter (ONSD), an indicator of intracranial pressure (ICP), and intraocular pressure (IOP) is controversial. The two aims of this study were; first, to investigate the effect of cerebrospinal fluid (CSF) estradiol (E2) and progesterone (P4) levels on ONSD and second, to investigate the relationship between ONSD and IOP in female dogs.</p><p><strong>Methods: </strong>IOP measurements were performed using rebound tonometer, and transpalpebral ultrasonographic assessment of the ONSD was conducted. CSF samples were collected, and E2 and P4 levels were quantified using enzyme-linked immunosorbent assay (ELISA). Multiple regression analysis was conducted to assess the relationships among the variables.</p><p><strong>Results: </strong>Multiple regression analyses on dogs found that the level of cerebrospinal fluid estradiol level (CSF-E2) significantly influenced left (L) ONSD (p = 0.0421, R² = 0.1551) and right (R) ONSD (p = 0.0216, R² = 0.1938). Systolic blood pressure (SBP) was a significant independent variable for left intraocular pressure (LIOP) (p = 0.0122, R² = 0.2261), while body weight was a significant predictor for right intraocular pressure (RIOP) (p = 0.0008, R² = 0.3679).</p><p><strong>Conclusions: </strong>This study revealed that CSF-E2 levels exert a significant influence on ONSD in dogs, whereas no direct association was observed between IOP and ONSD. These findings underscore the notion that ONSD is modulated not only by hemodynamic mechanisms but also by hormonal regulation.</p>","PeriodicalId":23690,"journal":{"name":"Veterinary Research Communications","volume":"49 5","pages":"255"},"PeriodicalIF":1.8,"publicationDate":"2025-07-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144609735","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Immunomodulatory and antioxidant responses of African catfish (Clarias gariepinus) to cestode infections: implications for intestinal health and aquaculture management.","authors":"Mahmoud Radwan, Jamila S Al Malki, Amaal Mohammadein, Mohamed Fares","doi":"10.1007/s11259-025-10819-4","DOIUrl":"https://doi.org/10.1007/s11259-025-10819-4","url":null,"abstract":"<p><p>Inadequate information exists on host-tapeworm parasite interactions in African catfish (Clarias gariepinus, Burchell 1822). Hence, this study evaluated the natural C. gariepinus severely infected mildly infected, on histopathological features, intestine health, immunological and antioxidant response to cestode parasites infection (Polyonchobothrium clarias (Woodland, 1925) Meggitt, 1930, Proteocephalus sp.). The prevalence of cestodes was 80%, with 26 and 52% of these fish mildly and severely infected, respectively. The prevalence was positively correlated with turbidity (0.64), NO₂ (0.58), NO₃ (0.63), and total alkalinity (0.57). Data illuminated that mildly and severely infected fish have more harmful effects on intestinal health and hemato-biochemical biomarkers than uninfected fish. Liver and intestinal antioxidant indices showed that the infected fish had higher malondialdehyde (MDA) levels than the uninfected fish. Correspondingly, mildly infected groups' liver and intestine showed significantly higher levels of superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GSH), while severely infected groups showed lower levels. Data clarified that infected fish have significantly upregulated pro-inflammatory genes IL-1β, Tlr1, and C3 expression, especially in mildly infected, than uninfected fish. However, the expression of CAT and SOD genes was significantly downregulated, in the severely infected, along with higher expression of the HSP70 gene than those of the uninfected groups. The histopathological study revealed that fish infected with the cestode parasite had inflammatory and abnormal features, especially in severely infected fish groups. Hence, the study concluded that the cestode parasites induce pathological immunological responses and health status in C. gariepinus.</p>","PeriodicalId":23690,"journal":{"name":"Veterinary Research Communications","volume":"49 5","pages":"254"},"PeriodicalIF":1.8,"publicationDate":"2025-07-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144609736","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correction to: Effects of central injection of liver-expressed antimicrobial peptide-2 (LEAP2) on feed intake in broiler chickens: interactions with opioidergic and serotonergic systems.","authors":"Kowsar Saffar, Morteza Zendehdel, Bita Vazir, Shahin Hassanpour, Kimia Mahdavi","doi":"10.1007/s11259-025-10824-7","DOIUrl":"https://doi.org/10.1007/s11259-025-10824-7","url":null,"abstract":"","PeriodicalId":23690,"journal":{"name":"Veterinary Research Communications","volume":"49 5","pages":"253"},"PeriodicalIF":1.8,"publicationDate":"2025-07-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144601674","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular epidemiology and phylogenetic analysis of Echinococcus spp. in the Qinghai-Tibetan Plateau: unraveling genetic evolution in a high-risk region for echinococcosis.","authors":"Guoyan Zhou, Zhi Li, Xueyong Zhang, Ru Meng, Xiuying Shen, Zhen Zhang, Fuyun Chen, Hong Duo, Yong Fu","doi":"10.1007/s11259-025-10815-8","DOIUrl":"https://doi.org/10.1007/s11259-025-10815-8","url":null,"abstract":"<p><p>As the largest and highest plateau in the world, the Qinghai-Tibetan Plateau (QTP) also represents a critical endemic region for echinococcosis, a zoonotic disease caused by Echinococcus spp. The disease is listed as a neglected tropical disease by the World Health Organization (WHO). Given its high prevalence and associated socioeconomic impacts, there is an urgent need to conduct in-depth research on this problem and formulate prevention and control intervention strategies. Here, we conducted a molecular genetic analysis of 1299 samples (including canid feces and livestock hydatid cysts) from the QTP based on the mitochondrial cytochrome c oxidase I (COI) gene. Our results revealed the infection rate of echinococcosis had some regional differences and identified 24 distinct haplotypes that clustered into five Echinococcus species. Encouragingly, the first isolation and identification of Echinococcus equinus in Ovis aries laid the foundation for the cross-species transmission of Echinococcus. Phylogenetic analyses (ML/BI) consistently supported highly clustering of each haplotype with its reference sequence (bootstrap ≥ 80%, PP ≥ 0.80). Furthermore, molecular clock estimates indicated that the co-evolution of Echinococcus spp. and its host may be closely related to the rapid uplift and climate changes of the QTP since the Cenozoic era. This study provided the latest dataset on Echinococcus infections in natural hosts across the QTP, systematically revealing the epidemic characteristics, genotype distribution and genetic diversity of Echinococcus spp. in this region.</p>","PeriodicalId":23690,"journal":{"name":"Veterinary Research Communications","volume":"49 5","pages":"252"},"PeriodicalIF":1.8,"publicationDate":"2025-07-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144601675","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Epidemiological assessment of bovine brucellosis in Iranian dairy farms: regional Seroprevalence and demographic indicators (2023-2024).","authors":"Alireza Bahonar, Akram Bahreinipour, Maryam Dadar","doi":"10.1007/s11259-025-10822-9","DOIUrl":"https://doi.org/10.1007/s11259-025-10822-9","url":null,"abstract":"<p><p>Brucellosis remains a significant challenge in Iran's dairy industry, with varying prevalence across provinces. This retrospective study (March 21, 2023-March 20, 2024) assessed bovine brucellosis seroprevalence across all regions of Iran (32 provinces), following Iranian Veterinary Organization (IVO) ethical guidelines. A total of 1,555,086 blood samples were collected from 612,558 dairy cattle at 4,586 industrial dairy farms, indicating that some animals were sampled more than once during the study period. Blood samples were screened using the Rose Bengal Plate Test, confirmed by Wright and 2-ME tests; milk samples were cultured for Brucella. Additionally, 248 milk samples were collected from seropositive farms. Overall, 7.6% (350 farms) had seropositive cases, with the highest rates in Qom (22.8%), Alborz (20.5%), Isfahan (18.1%), and Yazd (14.7%), which showed the highest farm-level prevalence. By contrast, provinces including South Khorasan (0.6%), West Azerbaijan (1%), and Kurdistan (1.1%) showed the lowest farm-level prevalence with positive cases. Provinces with zero positive cases at the farm level include Sistan and Baluchestan, Gilan, South Kerman, Mazandaran, and Hormozgan. Based on our study, the national average prevalence at the animal level in industrial dairy cattle is 0.4%, and the reactor to test percentage is 0.1%. Our analysis showed the highest animal-level prevalence in Qom (2.1%), Yazd (1.6%), and Fars (1.5%). Moreover, the highest reactor to test percentage was in Yazd (1.0%), followed by Qom and Khuzestan (0.5%). Holstein cattle were most affected (76.1%), followed by crossbreds (23%), with minimal infection in Simmental (0.7%) and Jersey (0.1%). Of the infected cattle, 79.6% were born on the farm, and 20.4% came from outside sources. Moreover, 82.2% of reactor cows were nursing, and 49.7% were pregnant at testing. Reproductive issues among positive reactors were noted: 17.1% had abortions, 1.3% had stillbirths, and 4% had weak calves. Vaccination records showed that 68.7% of reactors had received a full dose of the RB51 vaccine, while 31.3% were unvaccinated. Furthermore, 65% had been vaccinated with reduced doses of the RB51 vaccine, and 35% were unvaccinated. Brucella abortus and Brucella melitensis were found in 7.7% and 7.1% of milk samples, respectively. These results highlight the urgent need for stricter biosecurity, movement controls, and better vaccination to manage brucellosis in Iranian dairy farms.</p>","PeriodicalId":23690,"journal":{"name":"Veterinary Research Communications","volume":"49 5","pages":"251"},"PeriodicalIF":1.8,"publicationDate":"2025-07-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144592493","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Urinalysis in the white-eared opossum (Didelphis albiventris).","authors":"Fabiane de Holleben Camozzato Fadrique, Eduarda Aléxia Nunes Louzada Dias Cavalcanti, Lorena Eduarda Feitosa Ferrarezi da Silva, Roberto Gumieiro Junior, Matheus Giannechini Medeiros, Raqueli Teresinha França","doi":"10.1007/s11259-025-10818-5","DOIUrl":"https://doi.org/10.1007/s11259-025-10818-5","url":null,"abstract":"<p><p>To evaluate parameters and discuss findings of urinalysis conducted in non-captive, clinically healthy adult white-eared opossums (Didelphis albiventris). Twenty-five urine samples from different animals were analyzed: 15 from males and 10 from females, 14 obtained by spontaneous urination and 11 by cystocentesis. The analyses included physical, chemical, and sediment examinations. The results showed that 88% of the urine samples were citrine yellow, with urine specific gravity ranging from 1.019 to 1.084 and pH between 6 and 7. In the chemical analysis, there was no colorimetric reaction for parameters such as urobilinogen, bilirubin, blood, glucose, and ketone bodies, and few samples reacted for protein content. Regarding the sediment, red blood cells, white blood cells, and bacteria were observed in small numbers in most cases. No cylinders were found but triple phosphate crystals and bilirubin were detected in some samples. The results of urinalysis conducted in D. albiventris are similar to the data described in the literature for dogs and cats. Five cases of cystitis could be diagnosed, even though it was not possible to assess clinical signs, as done for domestic animals. These findings highlight the importance of a larger sample size to devise species-specific reference values. This work contributes to both future research and the clinical routine of professionals who treat this species.</p>","PeriodicalId":23690,"journal":{"name":"Veterinary Research Communications","volume":"49 5","pages":"248"},"PeriodicalIF":1.8,"publicationDate":"2025-07-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144585013","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Assessment of duodenal villous morphology in canine IBD using magnification endoscopy with water immersion technique (WIT) and image skeletonization.","authors":"Giulia Maggi, Laura Valli, Domenico Caivano, Elvio Lepri, Francesca Pirgher, Maria Chiara Marchesi","doi":"10.1007/s11259-025-10816-7","DOIUrl":"https://doi.org/10.1007/s11259-025-10816-7","url":null,"abstract":"<p><p>Inflammatory Bowel Disease (IBD) is an idiopathic disorder characterized by chronic, nonspecific gastrointestinal signs. Gastrointestinal endoscopy with histopathology is the gold standard for confirming inflammation and assessing severity. Although endoscopic criteria for IBD in dogs exist, detailed evaluation of duodenal villi morphology remains limited. In human medicine, magnification endoscopy with water immersion technique (WIT) and image skeletonization have proven useful for assessing villous atrophy in celiac disease. Only one veterinary study has applied these methods. This study aimed to assess the feasibility of WIT combined with skeletonized image analysis for evaluating duodenal villi in dogs with IBD. Thirty-three dogs undergoing endoscopy for suspected IBD were included. For each dog, five sub-images (7.5 × 7.5 mm²) were selected and skeletonized. The number of branches (B) and junctions (J) were quantified and correlated with histopathological inflammation grades. Skeletonized images before (nW) and after (W) WIT were compared based on average (AV) and maximum (Max) branch lengths. AV and Max branch lengths were significantly higher post-WIT. B and J values decreased with increasing severity of IBD. This study demonstrates that WIT and quantitative image analysis are simple, safe, and feasible for evaluating duodenal villous morphology in dogs with IBD. WIT could be routinely integrated into standard endoscopic procedures, offering immediate, standardized staging of IBD and enhancing diagnostic information.</p>","PeriodicalId":23690,"journal":{"name":"Veterinary Research Communications","volume":"49 5","pages":"249"},"PeriodicalIF":1.8,"publicationDate":"2025-07-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144585012","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Accidental monensin poisoning in buffaloes in Minas Gerais, Brazil.","authors":"Eduardo Bastianetto, Ariane Dantas","doi":"10.1007/s11259-025-10813-w","DOIUrl":"https://doi.org/10.1007/s11259-025-10813-w","url":null,"abstract":"<p><p>The manuscript reports accidental water buffalo (Bubalus bubalis) poisoning by monensin, a food supplement commonly used to feed cattle, and indicate some relevant aspects related to buffalo breeding. Most of the buffalo intoxication reports are accidental and occur in areas where buffalo breeding is not a traditional activity or in new buffalo breed without expert assistance. The present communication reports accidental buffalo poisoning due to monensin consumption at one corral that usually breed bovine to slaughter, a situation not reported before in Brazil. Monensin poisoning hypothesis was confirmed through investigation conducted by veterinarians using epidemiology, clinical signs and specific laboratory tests on food, blood and serum and biological samples obtained during a sick buffalo necropsy. Intoxicated animals presented apathy, anorexia, diarrhea, muscular weakness, locomotion disorders, dyspnea, tachycardia, jugular distension, pulse and recumbency, suggesting acute intoxication in the animals. Complementary diagnoses included myocarditis, moderate multifocal myonecrosis, myositis with lymphocytic infiltration and moderate multifocal oedema, presence of inflammatory biomarkers and normocytic normochromic anemia, typical of muscle damage observed in monensin poisoning.</p>","PeriodicalId":23690,"journal":{"name":"Veterinary Research Communications","volume":"49 5","pages":"250"},"PeriodicalIF":1.8,"publicationDate":"2025-07-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144585011","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Home blood pressure measurement in companion animals: underestimated or unknown?","authors":"Felipe Gaia de Sousa, Fabiana Silva Fádel Queiroz, Suzane Lilian Beier","doi":"10.1007/s11259-025-10812-x","DOIUrl":"https://doi.org/10.1007/s11259-025-10812-x","url":null,"abstract":"<p><p>Blood pressure (BP) measurement is a clinically significant procedure due to its close relationship with hemodynamics. Diseases that affect the components determining BP can lead to alterations in pressure values and must be recognized and managed therapeutically. Although BP can be measured in a hospital setting, it is well known that animals may exhibit signs of situational hypertension due to various factors, such as \"white coat syndrome,\" unfamiliar environments, distinct odors, long waiting times, contact with other animals, transportation, and other stress-inducing stimuli that can trigger the release of circulating catecholamines. As a result, temporary changes in BP readings may occur. If these factors are not considered when interpreting the results, it can lead to under- or overestimation of BP. Some professionals may overlook these conditions and frequently prescribe antihypertensive medications unnecessarily. In light of this, home BP monitoring is a potentially valuable strategy that could be incorporated into routine clinical practice, especially for patients who consistently present elevated BP values without an underlying condition to justify the increase. However, this technique remains underutilized, particularly in Brazil, contributing to high rates of inaccurate or erroneous diagnoses. Clearly defined protocols for performing blood pressure (BP) measurements at home are still necessary to ensure reliable and accurate diagnostics.</p>","PeriodicalId":23690,"journal":{"name":"Veterinary Research Communications","volume":"49 5","pages":"247"},"PeriodicalIF":1.8,"publicationDate":"2025-07-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144567914","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Gastrocnemius muscle rupture associated to selenium deficiency in beef cattle.","authors":"Raul E Marin, Carlos O Schild, Luiz G S Oliveira, Carlos Sant, Robert Poppenga, Francisco A Uzal, Franklin Riet-Correa","doi":"10.1007/s11259-025-10797-7","DOIUrl":"10.1007/s11259-025-10797-7","url":null,"abstract":"<p><p>Three cases of gastrocnemius muscle rupture occurred in cattle. The first two cases (Cases 1 and 2) occurred in a herd of 128, 16-18-month-old crossbred steers in Argentina; the lesions were bilateral. A third case (Case 3) occurred in a herd of 20, 2-year-old crossbred heifers in Uruguay; the lesion was unilateral. The three animals showed the tarsal joints flexed at an ~ 90° angle and the metatarsal bone region was touching the ground along its entire length. Necropsy and histopathology were conducted on Cases 1 and 3. Grossly, the gastrocnemius muscles were torn and edematous. Microscopically, the two animals had polyphasic, multifocal muscle necrosis with proliferation of satellite cells and macrophage infiltration. In Case 3 the selenium liver concentration was 0.29 ppm DW; selenium concentration was not measured in Cases 1 and 2. The gross and microscopic changes, coupled with low concentration of selenium in one of the animals, suggests that the gastrocnemius rupture was consequence of nutritional myopathy associated with selenium deficiency.</p>","PeriodicalId":23690,"journal":{"name":"Veterinary Research Communications","volume":"49 4","pages":"245"},"PeriodicalIF":1.8,"publicationDate":"2025-07-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144561325","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}