Veterinary Research Communications最新文献

Tissue distribution of Ocimum basilicum essential oil in Nile tilapia Oreochromis niloticus after anesthesia.

IF 1.8 3区农林科学

Veterinary Research Communications Pub Date : 2025-03-21 DOI: 10.1007/s11259-025-10716-w

Emilly Monteiro Lopes, Arlene Sobrinho Ventura, Claudia Andrea Lima Cardoso, Alexandre Vaz da Silva, Caio Francisco Santana Farias, Domickson Silva Costa, Ana Paula de Souza, Danilo Vitor Vilhena Batista, Silvia Terra Fontes, Gabriela Tomas Jerônimo, José Luiz Pedreira Mouriño, Maurício Laterça Martins

{"title":"Tissue distribution of Ocimum basilicum essential oil in Nile tilapia Oreochromis niloticus after anesthesia.","authors":"Emilly Monteiro Lopes, Arlene Sobrinho Ventura, Claudia Andrea Lima Cardoso, Alexandre Vaz da Silva, Caio Francisco Santana Farias, Domickson Silva Costa, Ana Paula de Souza, Danilo Vitor Vilhena Batista, Silvia Terra Fontes, Gabriela Tomas Jerônimo, José Luiz Pedreira Mouriño, Maurício Laterça Martins","doi":"10.1007/s11259-025-10716-w","DOIUrl":"https://doi.org/10.1007/s11259-025-10716-w","url":null,"abstract":"This study evaluated how the levels of methyl chavicol and linalool behave in tissues of juvenile Nile tilapia after anesthesia with the essential oil (EO) of Ocimum basilicum at different temperatures. The EO was diluted in ethanol (1:9), and the fish (64.99 ± 13.90 g) were immersed in the anesthetic solution (250 mg L-1) at 23 and 27 ºC for 10 min. After anesthesia, the fish were placed in tanks free of anesthetic solution for recovery. Tissues (gill, brain, liver and muscle) were collected at 0, 0.5, 3, 6, and 12 h after anesthesia. The compounds were quantified in the gills, brain and liver up to 6 h, and in the muscle up to 12 h at both temperatures. The maximum concentration (Cmax) of both compounds was highest in the liver at 23 ºC and lowest in the muscle at 27 ºC. In the gills, the Cmax of methyl chavicol and linalool was highest at 23 ºC with half-lives of 0.78 h and 0.70 h, respectively. The levels of methyl chavicol and linalool in the brain were similar at 23 and 27 ºC. In the liver, elimination of the compounds was more efficient at 27 ºC, with a significant interaction between temperature and depletion. In the muscle, the compounds showed greater retention, with slower elimination compared to the other tissues. The temperature of 23 ºC favored greater absorption of the compounds in the gills and liver, while 27 ºC accelerated their elimination. The brain and muscle, on the other hand, were not influenced by temperature. The liver was identified as the main organ for metabolizing the compounds, with less elimination in the muscle. The results highlight the impact of temperature on the metabolism of anesthetics in fish.","PeriodicalId":23690,"journal":{"name":"Veterinary Research Communications","volume":"49 3","pages":"148"},"PeriodicalIF":1.8,"publicationDate":"2025-03-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143671084","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Dietary methanol extract of purslane (Portulaca oleracea L.) can help reduce the negative impacts of triiodothyronine-induced ascites in broiler chickens.

IF 1.8 3区农林科学

Veterinary Research Communications Pub Date : 2025-03-20 DOI: 10.1007/s11259-025-10723-x

Mahmood Habibian, Amirali Sadeghi, Ahmad Karimi

{"title":"Dietary methanol extract of purslane (Portulaca oleracea L.) can help reduce the negative impacts of triiodothyronine-induced ascites in broiler chickens.","authors":"Mahmood Habibian, Amirali Sadeghi, Ahmad Karimi","doi":"10.1007/s11259-025-10723-x","DOIUrl":"https://doi.org/10.1007/s11259-025-10723-x","url":null,"abstract":"This study investigated the effects of purslane methanol extract (PME) on broilers suffering from triiodothyronine (T3)-induced ascites. A total of 240 one-day-old chicks were divided into four dietary treatment groups: a control diet, a T3 diet (1.5 mg/kg), a T3 diet added with 150 mg/kg PME, and a T3 diet added with 300 mg/kg PME. Results indicated that the T3-treated birds exhibited a higher right ventricle to total ventricle (RV/TV) ratio and increased mortality due to ascites, alongside reduced feed intake, body weight gain, and production efficiency index, while showing a higher feed conversion ratio compared with controls. Despite the lack of significant enhancement in growth performance due to dietary PME supplementation, the group that was administered a diet with 300 mg/kg PME demonstrated an improved production efficiency index, as well as decreased mortality and a reduced RV/TV ratio. Additionally, the birds subjected to T3 treatment displayed diminished activity of antioxidant enzymes (superoxide dismutase, catalase, and glutathione peroxidase), in conjunction with elevated malondialdehyde levels compared with the control birds. Supplementation with 300 mg/kg PME increased antioxidant enzyme activities and lowered malondialdehyde concentrations to near control levels. Furthermore, T3 treatment led to elevated red blood cell counts, hematocrit, hemoglobin, and heterophil to lymphocyte ratio, which were significantly mitigated by PME supplementation. To conclude, PME effectively mitigates the adverse effects of T3-induced ascites in broilers by enhancing antioxidant enzyme activity and improving production efficiency, suggesting its potential as a dietary supplement in poultry production.","PeriodicalId":23690,"journal":{"name":"Veterinary Research Communications","volume":"49 3","pages":"147"},"PeriodicalIF":1.8,"publicationDate":"2025-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143671083","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

First identification and molecular characterization of protozoan parasites associated with abortion in ruminants from South Sinai Governorate, Egypt.

IF 1.8 3区农林科学

Veterinary Research Communications Pub Date : 2025-03-19 DOI: 10.1007/s11259-025-10698-9

Safaa Mohamed Barghash, Al-Shaimaa Mohsen Sadek

{"title":"First identification and molecular characterization of protozoan parasites associated with abortion in ruminants from South Sinai Governorate, Egypt.","authors":"Safaa Mohamed Barghash, Al-Shaimaa Mohsen Sadek","doi":"10.1007/s11259-025-10698-9","DOIUrl":"https://doi.org/10.1007/s11259-025-10698-9","url":null,"abstract":"This study presents the first molecular detection and characterization of protozoa associated with livestock abortion, including Toxoplasma gondii, Neospora caninum, Sarcocystis spp., and Microsporidia spp., in the South Sinai Governorate, Egypt. A total of 226 blood samples were collected from cattle, goats, and sheep with a history of abortion. Molecular analysis was conducted using PCR and sequencing of the internal transcribed spacer (ITS) region of the rRNA gene. The results revealed the prevalence of T. gondii in cattle at 12.33% (9/73), while goats and sheep tested negative. In contrast, 14.92% (10/67) of goat samples tested positive for Microsporidia spp., with no cases detected in cattle or sheep. No infections with Sarcocystis spp. or N. caninum were identified in any tested animals. Across all examined livestock, the overall prevalence of T. gondii was 3.98% (9/226), while Microsporidium spp. exhibited a slightly higher prevalence at 4.42% (10/226). Molecular sequencing identified three novel T. gondii strains in cattle recorded in the GenBank database under accession numbers MZ197902, MZ197903, and MZ197904, showing 99.9% genetic similarity to reference strains. Additionally, three novel strains of Enterocytozoon bieneusi (MZ197781, MZ197782, and MZ197783) were detected in goats. Phylogenetic analysis indicated a close evolutionary relationship between these newly identified E. bieneusi strains and known genotypes, suggesting a high degree of genetic conservation. These findings underscore the ongoing risks of T. gondii and E. bieneusi to livestock and public health. Continued surveillance is necessary to assess the zoonotic potential of these novel strains to aid in effective management strategies.","PeriodicalId":23690,"journal":{"name":"Veterinary Research Communications","volume":"49 3","pages":"145"},"PeriodicalIF":1.8,"publicationDate":"2025-03-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143664633","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

First report of Moraxella oculi in Brazil in an infectious bovine keratoconjunctivitis outbreak.

IF 1.8 3区农林科学

Veterinary Research Communications Pub Date : 2025-03-19 DOI: 10.1007/s11259-025-10713-z

Clarissa Vidal de Carvalho, Robert Domingues, Cinthia de Carvalho Coutinho, Nicole Tafnes de Brito Silva Honório, Daniele Ribeiro de Lima Reis Faza, Alessandra Barbosa Ferreira-Machado, Wanessa Araújo Carvalho, Emanuelle Baldo Gaspar, Marta Fonseca Martins

{"title":"First report of Moraxella oculi in Brazil in an infectious bovine keratoconjunctivitis outbreak.","authors":"Clarissa Vidal de Carvalho, Robert Domingues, Cinthia de Carvalho Coutinho, Nicole Tafnes de Brito Silva Honório, Daniele Ribeiro de Lima Reis Faza, Alessandra Barbosa Ferreira-Machado, Wanessa Araújo Carvalho, Emanuelle Baldo Gaspar, Marta Fonseca Martins","doi":"10.1007/s11259-025-10713-z","DOIUrl":"https://doi.org/10.1007/s11259-025-10713-z","url":null,"abstract":"Infectious Bovine Keratoconjunctivitis (IBK) is a widespread ocular disease that affects dairy and beef cattle worldwide, caused by Gram-negative bacteria from the genus Moraxella. It is the most common eye disease in cattle, with symptoms including tearing, ocular pain, corneal opacity, photophobia, ulceration, and, in severe cases, permanent blindness. This study focused on characterizing Moraxella species in a 2022 IBK outbreak in Minas Gerais, Brazil. Ocular swabs from 18 symptomatic Holstein cattle were analyzed through colony isolation, physiological tests, and molecular techniques, including PCR-RFLP and sequencing. Results revealed one isolate of Moraxella bovoculi genotype 1 and five isolates of Moraxella oculi, marking the first report of the latter species in Brazil. This study represents the second report of Moraxella oculi isolation and the first in a country different from the initial report. Notably, Moraxella bovis was not isolated in this outbreak. All isolates exhibited susceptibility to the tested antibiotics. Comparative genomic analysis between the Brazilian Moraxella oculi isolate and the American strain (Tifton 1) revealed 99.5% similarity in the 16-23 S rRNA locus and 99.0% average nucleotide identity.","PeriodicalId":23690,"journal":{"name":"Veterinary Research Communications","volume":"49 3","pages":"143"},"PeriodicalIF":1.8,"publicationDate":"2025-03-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143664635","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Fecal strains Enterococcus mundtii from wild ruminants, their safety and postbiotic potential.

IF 1.8 3区农林科学

Veterinary Research Communications Pub Date : 2025-03-19 DOI: 10.1007/s11259-025-10701-3

Andrea Lauková, Valentína Focková, Marián Maďar, Grzegorz Belzecki, Renata Miltko, Monika Pogány Simonová

{"title":"Fecal strains Enterococcus mundtii from wild ruminants, their safety and postbiotic potential.","authors":"Andrea Lauková, Valentína Focková, Marián Maďar, Grzegorz Belzecki, Renata Miltko, Monika Pogány Simonová","doi":"10.1007/s11259-025-10701-3","DOIUrl":"https://doi.org/10.1007/s11259-025-10701-3","url":null,"abstract":"Roe and red deers represent inhabitants in European forests but also in agricultural areas. In general, microbiota can have a significant impact on health. E. g. the genus Enterococcus was detected in more or less high abundance percentage in feces of red deers. To preserve negative impact of some microbiota, beneficial autochthonous strains can be selected for this purpose. The aim of this study was to assess safety, postbiotic activity and character of the fecal strains Enterococcus mundtii from roe and red deers living in Poland to spread basic microbiology research in this field and to select application candidate. Taxonomy of seven E. mundtii was determined using MALDI-TOF mass spectrometry and 16S rRNA sequence analysis. The evaluation score responded mostly with secure genus identification/probable species identification. Strains showed identity up to 100% with the sequence MK414812.1 of E. mundtii in GenBank. They were deoxyribonuclease and gelatinase- negative, with low-grade biofilm formation (0.100 ± 0.31 to 0.181 ± 0.43). E. mundtii were susceptible to antibiotics without production of damaging enzymes. They were absent of virulence factors genes (gelE, agg, esp, efaAfm, efaAfs). Postbiotic potential of the bacteriocin substance produced by E. mundtii revealed inhibition of indicator strains growth up to 48%. The most active substance was from the strain EM 1/90/2; inhibitory activity against enterococci, staphylococci and listeriae up to 86% (up to 1600 AU mL) was noted. The substance from the strain EM 6/123/1 reached inhibitory activity up to 81% with activity up to 400 AU/mL. It remained active at -20 ˚C for one month. E. mundtii were assessed with no and/or low- grade virulence factor rate and with postbiotic potential for further studies.","PeriodicalId":23690,"journal":{"name":"Veterinary Research Communications","volume":"49 3","pages":"141"},"PeriodicalIF":1.8,"publicationDate":"2025-03-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143664620","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Total knee replacement in a dog with an articular epiphyseal medial condylar fracture of the distal femur, a case report.

IF 1.8 3区农林科学

Veterinary Research Communications Pub Date : 2025-03-19 DOI: 10.1007/s11259-025-10712-0

Brent R Fink, Laurent P Guiot, Peter Muir

引用次数: 0

Detection and genomic characterization of foot and mouth disease virus SAT2 XIV topotype using amplicon-based nanopore sequencing.

IF 1.8 3区农林科学

Veterinary Research Communications Pub Date : 2025-03-19 DOI: 10.1007/s11259-025-10718-8

Ibrahim H M Abualghusein, Mustafa M K Ababneh, Mohammad Borhan F Al-Zghoul, Daoud A A Alghizzawi, Hebah A A Aboomer

{"title":"Detection and genomic characterization of foot and mouth disease virus SAT2 XIV topotype using amplicon-based nanopore sequencing.","authors":"Ibrahim H M Abualghusein, Mustafa M K Ababneh, Mohammad Borhan F Al-Zghoul, Daoud A A Alghizzawi, Hebah A A Aboomer","doi":"10.1007/s11259-025-10718-8","DOIUrl":"https://doi.org/10.1007/s11259-025-10718-8","url":null,"abstract":"Foot and mouth disease (FMD) is a highly contagious vesicular viral disease that infects cloven-hoofed animals. This disease is caused by Foot-and-mouth disease virus (FMDV) which is composed of seven serotypes (O, A, C, Asia-1, SAT1, SAT2, and SAT3), each of which lacks cross immune protection among serotypes which complicates vaccination strategies. For that, sequencing of viral protein 1 (VP1) is crucial to identify circulating serotypes in a country. Jordan is endemic for FMD, with primarily O and A serotypes circulating according to previous data. However, the epidemiological status of FMD requires updates in Jordan. In this study, out of 200 samples collected during a recent outbreak in Al-Dhlail, Jordan in early 2023, forty-nine were positive by quantitative polymerase chain reaction (qPCR). Fourteen of these positive samples were selected for PCR amplification of the viral protein 1 (VP1) gene, which were then sequenced using nanopore technology. Phylogenetic analysis highlighted the presence of SAT2 XIV topotype, with eleven samples successfully sequenced using this approach. In addition to that, Sanger sequencing validated a N137S SNP in the VP1 protein that initially identified by our approach. This study provides additional data that can be used and incorporated into the national and global efforts against this disease with new means of diagnostics methods.","PeriodicalId":23690,"journal":{"name":"Veterinary Research Communications","volume":"49 3","pages":"144"},"PeriodicalIF":1.8,"publicationDate":"2025-03-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143664597","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Theileria parva genetics, prevalence and vaccination practices in Zimbabwe and the African region and the prospects for vaccine development: a systematic review.

IF 1.8 3区农林科学

Veterinary Research Communications Pub Date : 2025-03-19 DOI: 10.1007/s11259-025-10715-x

Daniel Mukandabvute, Noah Herbert Paul, Fanuel Songwe, Maud Chipatiko, Liana-Lisa Sakwa, Nyasha Chin'ombe, Leonard Madzingaidzo

{"title":"Theileria parva genetics, prevalence and vaccination practices in Zimbabwe and the African region and the prospects for vaccine development: a systematic review.","authors":"Daniel Mukandabvute, Noah Herbert Paul, Fanuel Songwe, Maud Chipatiko, Liana-Lisa Sakwa, Nyasha Chin'ombe, Leonard Madzingaidzo","doi":"10.1007/s11259-025-10715-x","DOIUrl":"https://doi.org/10.1007/s11259-025-10715-x","url":null,"abstract":"Introduction: January disease causes the deaths of over 55,000 cattle valued at approximately US$ 17 million annually in Zimbabwe. The locally developed Boleni stabilate vaccine is in use for controlling the disease. In the present review, we show the current knowledge of the genetic variation and population structure of Theileria parva parasite and its implications on the epidemiology and control of the parasite in eastern and southern Africa, with a major emphasis on Zimbabwe.Methods: A systematic review was conducted to analyse the reports available in literature in order to map the Theileria parva genetic diversity profile. A total of 103 studies met the criteria for analysis and were included in the review.Results: The reports retrieved in this study show that East Coast fever and Corridor disease have been extensively sequenced to establish the parasite population genetic structure. One report described genetic diversity in January disease, with no sequencing tools included. The live sporozoite stabilate administered as infection without concurrent treatment remains the vaccination approach of choice in January disease.Discussion: The adoption of population genetics led to the determination of genotypes that were employed in the development of the Muguga cocktail vaccine. To date, vaccination against East Coast fever is reported to reduce mortality rate. The Boleni isolate remains in use for vaccination against uncharacterized populations of January disease in spite of increased mortality rates extensively reported from 2015 to date. There is need to adopt comparative genomics in vaccine development for a more effective vaccine.","PeriodicalId":23690,"journal":{"name":"Veterinary Research Communications","volume":"49 3","pages":"146"},"PeriodicalIF":1.8,"publicationDate":"2025-03-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143664641","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of dietary phytase and sex on behavioral responses in castrated male and female piglets. 日粮植酸酶和性别对阉割公母猪行为反应的影响

IF 1.8 3区农林科学

Veterinary Research Communications Pub Date : 2025-03-13 DOI: 10.1007/s11259-025-10714-y

Gustavo Zigovski, Elmer Modesto Elvir Hernandez, Isabela Cristina Colaço Bez, Gabriel Kioshi Cavalari Nakamura, Mariana Regina Rosa Catoia, Ana Julia Carrasco Buzatto, Ruan Rolnei Daros, Paulo Levi de Oliveira Carvalho, Leandro Batista Costa

{"title":"Effects of dietary phytase and sex on behavioral responses in castrated male and female piglets.","authors":"Gustavo Zigovski, Elmer Modesto Elvir Hernandez, Isabela Cristina Colaço Bez, Gabriel Kioshi Cavalari Nakamura, Mariana Regina Rosa Catoia, Ana Julia Carrasco Buzatto, Ruan Rolnei Daros, Paulo Levi de Oliveira Carvalho, Leandro Batista Costa","doi":"10.1007/s11259-025-10714-y","DOIUrl":"https://doi.org/10.1007/s11259-025-10714-y","url":null,"abstract":"This study investigated whether adding phytase to the diet of castrated male and female piglets modulates behavior and if the response differs between sexes. The experiment involved 126 piglets, weaned at 21 days of age, with an average weight of 6.53 ± 1.29 kg. Six treatments were employed, with seven replications and three animals per pen. The treatments were PC: positive control diet - adequate nutritional levels and without phytase; NC: negative control diet - reduced levels of nutrients and metabolizable energy, and without phytase; negative control diets with 500; 1.000; 1.500 and 2.000 FTU/kg of feed. Piglets were subjected to open field (OF), novel object (NO) and reactivity during weighing (RDW) behavioral tests. In the OF, females showed longer exploration time (p = 0.036) and explored a greater number of quadrants (p = 0.031). Conversely, males remained more static (p < 0.01) and vocalized more (p = 0.041). For the NO, females exhibited shorter latency to reach the novel object (p = 0.040). In the RDW, male piglets exhibited greater movement resistance (p = 0.011) and vocalized more frequently (p = 0.038). The addition of phytase did not affect piglet behavior; however, the results suggest that females are more explorative, while castrated males show increased immobility in response to fear.","PeriodicalId":23690,"journal":{"name":"Veterinary Research Communications","volume":"49 3","pages":"139"},"PeriodicalIF":1.8,"publicationDate":"2025-03-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143626046","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of intramammary infusion of Minthostachys verticillata essential oil in cows at drying-off on microbiological and immunological parameters, and milk quality during the subsequent lactation.

IF 1.8 3区农林科学

Veterinary Research Communications Pub Date : 2025-03-13 DOI: 10.1007/s11259-025-10709-9

Sofía Arsaute, Elina B Reinoso, María E Cecchini, Ivana D Montironi, Dardo A Roma, Fabiana D Éramo, Marcela Moressi, Lorena Decara, Javier Macchiavelli, Andrea Ariño, Laura Noelia Cariddi

{"title":"Effects of intramammary infusion of Minthostachys verticillata essential oil in cows at drying-off on microbiological and immunological parameters, and milk quality during the subsequent lactation.","authors":"Sofía Arsaute, Elina B Reinoso, María E Cecchini, Ivana D Montironi, Dardo A Roma, Fabiana D Éramo, Marcela Moressi, Lorena Decara, Javier Macchiavelli, Andrea Ariño, Laura Noelia Cariddi","doi":"10.1007/s11259-025-10709-9","DOIUrl":"https://doi.org/10.1007/s11259-025-10709-9","url":null,"abstract":"This study evaluated the effects of EO administered via intramammary inoculation in dairy cows during the drying-off period and its impact on milk quality in the subsequent lactation. Ten pregnant Holstein cows, free of mastitis, were selected from an organic dairy farm in Córdoba, Argentina. Twenty-four quarters were inoculated with EO: eight received 0.25 g, eight received 0.50 g, and eight received 1.00 g. Control groups included uninoculated quarters and quarters treated with cephapirin. Milk samples were collected before treatment and within 4 days after calving to assess microbial count and somatic cell counts (SCC). The expression of TNF-α, IL-6, and IL-10 was quantified using qPCR and malondialdehyde (MDA) levels were also measured. Blood samples were collected before treatment and within 4 days after calving to determine serum IL-6 and IL-4 levels using ELISA. No significant effects were observed on milk's physical-chemical properties following EO or cephapirin treatment. EO at 1.00 g significantly reduced microbial load (p < 0.05), comparable to cephapirin. SCC increased with all EO doses (p < 0.01, p < 0.05, p < 0.05, respectively). Differential cell counts showed an increase in macrophages after 1.00 g EO treatment. The relative expression of TNF-α, IL-6, and IL-10 decreased without statistical significance. MDA levels significantly increased with cephapirin (p < 0.001), but EO had no effect. Serum IL-6 and IL-4 levels remained unchanged. In conclusion, intramammary EO inoculation enhanced immune response, reduced microbial load, and did not induce lipid peroxidation, suggesting its potential as a preventive treatment for mastitis.","PeriodicalId":23690,"journal":{"name":"Veterinary Research Communications","volume":"49 3","pages":"140"},"PeriodicalIF":1.8,"publicationDate":"2025-03-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143626238","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0