Veterinary Research Communications最新文献

{"title":"Fumonisin B1 induces oxidative stress, inflammation and necroptosis in IPEC-J2 cells.","authors":"Yinuo Zou, Xinyu Du, Xiaoyan Zheng, Sichao Mao, Xinyi Xu, Shuxia Zhang, Xingxiang Chen","doi":"10.1007/s11259-025-10728-6","DOIUrl":"https://doi.org/10.1007/s11259-025-10728-6","url":null,"abstract":"<p><p>Fumonisin B1 (FB1), an important mycotoxin, poses a significant threat to public health and livestock production due to its widespread contamination. Furthermore, the gastrointestinal tract is particularly vulnerable to FB1 exposure given its frequent contamination of staple crops such as corn. Although necroptosis has been recognized as a critical mechanism underlying intestinal damage caused by certain environmental toxins, whether FB1 specifically triggers necroptosis in intestinal epithelial cells remains to be fully elucidated. In this study, the intestinal porcine epithelial cell line-J2 (IPEC-J2) was employed as an in vitro model to study the intestinal cells injury caused by FB1 and the underlying mechanisms. By measuring IPEC-J2 cell viability, intracellular reactive oxygen species, gene levels, and protein levels, it was found that FB1 dose-dependent induced IPFC-J2 cell injury, oxidative stress, and inflammation. Meanwhile, FB1 significantly increased the expression of necroptosis-related genes and proteins in IPEC-J2 cells, indicating that FB1 induced the occurrence of necroptosis. In summary, the results demonstrated FB1 can induce oxidative stress, inflammation and necroptosis in IPEC-J2 cells.</p>","PeriodicalId":23690,"journal":{"name":"Veterinary Research Communications","volume":"49 3","pages":"161"},"PeriodicalIF":1.8,"publicationDate":"2025-04-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143812513","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bacillus velezensis MFF 2.2-derived lipopeptides modulate gut immune response in BALB/c mice maintaining mucosal integrity and systemic safety.","authors":"Ana Lucía Di Giácomo, Lorena Nadia Azcurra, Gisela Romina García, José María Raviolo, María Carolina Grosso, Cecilia Ana Dogi, María Laura González Pereyra","doi":"10.1007/s11259-025-10730-y","DOIUrl":"https://doi.org/10.1007/s11259-025-10730-y","url":null,"abstract":"<p><p>Bacillus-derived lipopeptides, particularly surfactin (SF), are known for their antimicrobial and immunomodulatory properties, making them promising candidates for applications in veterinary medicine. This study investigated the immunomodulatory, cytotoxic, and genotoxic effects of a lipopeptide extract (LPE) derived from Bacillus velezensis MFF 2.2, containing SF, using an in vivo murine model. Four groups of BALB/c mice (n = 6 per group) were orally administered daily doses of: (1) saline solution (control), (2) 60 μg/mL SF standard, (3) 40 μg/mL LPE, and (4) 60 μg/mL LPE, over a 10-day period. Assessments included changes in body weight, histopathological analysis of liver, kidney, and small intestine tissues, as well as evaluation of cytotoxic and genotoxic effects. LPE treatment was well-tolerated, with no significant changes in body weight, organ histopathology, or bone marrow toxicity. Both SF and LPE significantly increased goblet cell counts (P < 0.05), while LPE reduced IFN-γ levels and increased the IL- 10/IFN-γ ratio, suggesting an anti-inflammatory response (P < 0.05). TNF-α and IL- 10 levels remained unchanged, and no significant differences were observed in intraepithelial lymphocyte counts. These findings suggest that LPE derived from B. velezensis MFF 2.2 is safe and promotes immunomodulatory effects, supporting its potential as a therapeutic agent in inflammatory conditions.</p>","PeriodicalId":23690,"journal":{"name":"Veterinary Research Communications","volume":"49 3","pages":"159"},"PeriodicalIF":1.8,"publicationDate":"2025-04-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143796463","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Seroepidemiological study of Leishmania infantum, Toxoplasma gondii and Dirofilaria immitis in pet ferrets (Mustela putorius furo) in Spain.","authors":"José Villora, María Eugenia Lebrero, Jacobo Giner, Asier Basurco, Laura Vilalta, Andrés Montesinos, Maria-Magdalena Alcover, Cristina Riera, Roser Fisa, Xavier Roca-Geronès, Aitor Ramos, Álex Gómez, Sergio Villanueva-Saz, Antonio Fernández, Diana Marteles","doi":"10.1007/s11259-025-10729-5","DOIUrl":"10.1007/s11259-025-10729-5","url":null,"abstract":"<p><p>Ferrets are susceptible to infections with Leishmania infantum, Toxoplasma gondii, and Dirofilaria immitis; however, epidemiological data on these infections are limited in Europe. A total of 448 serum samples from household ferrets were collected between December 2019 and December 2023 in Spain. In this study, we assessed the seroprevalence of L. infantum and D. immitis using an in-house enzyme-linked immunosorbent assay (ELISA) and T. gondii using an in-house immunofluorescence antibody test (IFAT). Among the ferrets tested, the seroprevalence was 10.49% (47/448) for L. infantum, 2.68% (12/448) for T. gondii and 10.27% (46/448) for D. immitis. There was no significant association between seropositivity and age, gender, neutering status, cohabitation, lifestyle, and collection date. Ferrets classified as sick animals related to the presence of compatible or non-compatible signs showed a higher seropositivity rate for L. infantum (15.90%) compared to subclinical animals (4.76%). Overall, 23.44% (105/448) of the samples were seropositive for at least one of the three parasitic agents, 3.12% (15/448) were positive for two agents, and 0.22% (1/448) tested positive for all three agents. Co-infections were also evaluated, revealing that 12.76% (6/47) of L. infantum seropositive ferrets were also positive for T. gondii (p = 0.011) and 21.27% (10/47) for D. immitis (p = 0.009). To the best of our knowledge, this is the first report on the seroprevalence of L. infantum, D. immitis, and T. gondii within the ferret population in Spain. Understanding the epidemiological status of these and other zoonotic pathogens is crucial for enhancing surveillance in both veterinary and public health sectors, as well as for strengthening prevention and control strategies.</p>","PeriodicalId":23690,"journal":{"name":"Veterinary Research Communications","volume":"49 3","pages":"160"},"PeriodicalIF":1.8,"publicationDate":"2025-04-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143796467","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impact of single versus multiple infection on serum protein fractions in cats.","authors":"Diana Marteles, María Eugenia Lebrero, Antonio Fernández, Aurora Ortín, Ana González, Carmen Morell, María Jesús Villanueva, Ingo Schäfer, Pablo Quílez, Maite Verde, Alex Gómez, Sergio Villanueva-Saz","doi":"10.1007/s11259-025-10724-w","DOIUrl":"10.1007/s11259-025-10724-w","url":null,"abstract":"<p><p>Serum protein electrophoresis (SPE) is a widely used diagnostic tool for identifying acute and chronic inflammation, as well as immunodeficiencies. However, the impact of co-infections on SPE patterns in cats remains poorly understood. This study explored the utility of SPE in differentiating immune responses between cats infected with a single pathogen and those with multiple co-infections. A total of 79 serum samples from stray European Shorthair cats in Zaragoza, Spain, were analyzed. Fifty cats had a single infection, while 29 were co-infected with 2-4 pathogens. Agarose gel electrophoresis was used to assess protein profiles and statistical analyses were performed to identify significant differences between groups. The results showed no major differences in protein profiles between single and co-infected cats, with polyclonal hypergammaglobulinemia being most common in single-pathogen infections. Therefore, these findings indicate that SPE may have limitations in distinguishing between single and multiple infections in cats, contrasting with some previous studies.</p>","PeriodicalId":23690,"journal":{"name":"Veterinary Research Communications","volume":"49 3","pages":"158"},"PeriodicalIF":1.8,"publicationDate":"2025-04-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11971170/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143781284","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Study of epidemiological and molecular characteristics of Brucella strains circulating in Kazakhstan.","authors":"Aida Daugaliyeva, Saule Daugaliyeva, Aspen Abutalip, Akmaral Adambayeva, Nazerke Kydyr, Simone Peletto","doi":"10.1007/s11259-025-10725-9","DOIUrl":"10.1007/s11259-025-10725-9","url":null,"abstract":"<p><p>Brucellosis is one of the most common zoonotic infectious diseases of animals. The causative agent of brucellosis is a highly conserved bacterium of the genus Brucella, which includes 14 species with minor genetic changes. Our work aimed to study the epidemiological and molecular characteristics of Brucella strains circulating in Kazakhstan, including the study of polymorphisms in critical virulence genes using single nucleotide polymorphism (SNP) analysis based on whole genome sequencing (WGS). A total of 21 Brucella isolates obtained in 2023-2024 from cattle, sheep and goats, horses, camels, pigs, dogs, and humans were analyzed. As a result of whole-genome sequencing, 69 virulence genes were detected. The genome of Brucella melitensis, the most virulent species of Brucella, was the most susceptible to changes. Among the 10 virulence genes studied using SNP, polymorphisms were found in 7 genes. Some genetic mutations resulted in amino acid changes in the coding sequences of these virulence genes. Knowledge of virulence factors and changes in their coding genes has practical implications for epidemiological studies in outbreak areas, their management, and the implementation of brucellosis control strategies.</p>","PeriodicalId":23690,"journal":{"name":"Veterinary Research Communications","volume":"49 3","pages":"156"},"PeriodicalIF":1.8,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11961523/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143754773","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genetic diversity and population structure of Fasciola gigantica isolated from cattle in Malawi.","authors":"Lenson Mogha, Henson Kainga, Nathan Kamanga, Thoko Flav Kapalamula, Catherine Wood, Lian F Thomas, Florence Mutua, Neil Sargison, Kyoko Hayashida, Taiga Tsutsumi, Naoki Hayashi, Nariaki Nonaka, Ryo Nakao, Elisha Chatanga","doi":"10.1007/s11259-025-10717-9","DOIUrl":"https://doi.org/10.1007/s11259-025-10717-9","url":null,"abstract":"<p><p>Fasciola gigantica is an important trematode that affects the health of animals and humans in tropical and subtropical countries, including Malawi. Information on the genetic diversity and population structure of F. gigantica is important to understanding the parasite`s transmission patterns/ and in monitoring the development of resistance to commonly used anthelmintic agents. This study aimed to analyze the genetic diversity and population structure of Fasciola species collected from cattle at slaughter slabs and abattoirs in selected districts of Malawi. A total of 27 adult liver flukes were collected from cattle at slaughter slabs and abattoirs in the northern region (n = 12), central region (n = 5), and southern region (n = 10). The mitochondrial cytochrome c oxidase I (COI) gene and nicotinamide adenine dinucleotide dehydrogenase 1 (ND1) gene were amplified and the amplicons were sequenced for all samples. The sequences obtained were used to investigate genetic diversity through median-joining networks and phylogenetic analysis. Tajima's D test and Fu's Fs statistics were used to determine the population structure. Based on the analyzed COI and ND1 sequences, all samples were identified as F. gigantica. Single nucleotide polymorphisms (SNPs) were identified at 18 and 17 positions for COI and ND1 genes, resulting in 10 and 5 haplotypes, respectively. The haplotype diversities were 0.867 and 0.479 for COI and ND1 gene sequences, respectively. The population genetic structure indices showed a population that has undergone a recent expansion. This study provides baseline epidemiological data on the genetic diversity and population structure of F. gigantica in Malawi; which is important for its control.</p>","PeriodicalId":23690,"journal":{"name":"Veterinary Research Communications","volume":"49 3","pages":"157"},"PeriodicalIF":1.8,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143754761","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Clostridium septicum-associated myonecrosis in a dog: case report.","authors":"Isabele Maurer Amorim, Luiz Gustavo Schneider de Oliveira, Fabiana Marques Boabaid, Andressa da Silva Martins, Leidiane de Souza Gomes, Fernanda Martins Paixão, Ingrid Carneiro de Oliveira Falcão, Danieli Brolo Martins, Rosângela de Oliveira Alves Carvalho, Aline Maria Vasconcelos Lima, Ana Carolina Borsanelli","doi":"10.1007/s11259-025-10726-8","DOIUrl":"https://doi.org/10.1007/s11259-025-10726-8","url":null,"abstract":"<p><p>Necrotizing soft tissue diseases are severe, rapidly progressive infections characterized by tissue destruction and toxemia, often leading to death. Although common in livestock, myonecrosis caused by toxinogenic clostridial species is rarely reported in dogs. This study reports a case of myonecrosis in a male mongrel dog attacked by stray dogs, which subsequently developed a Clostridium septicum infection. The dog, prostrate and in contact with the ground, was brought for treatment two days post-attack, presenting multiple bite wounds, particularly on the pelvic limbs and scrotum, and a scrotal abscess. The condition progressed to toxemic shock, with extensive necrosis and emphysema in subcutaneous tissue and muscles. Due to clinical deterioration, euthanasia was performed. Postmortem findings revealed widespread edema, hemorrhage, and emphysema in the affected skeletal muscle and subcutaneous tissue of the pelvic limbs and scrotum, alongside moderate multifocal purulent exudate in the muscle fascia. Histological analysis identified myonecrosis with perimysium and subcutaneous tissue expansion due to hemorrhage, proteinaceous material, and emphysema. Suppurative infiltrates containing gram-positive, rod-shaped bacteria were present within the fascia. PCR analysis confirmed C. septicum in samples of affected skeletal muscle. This case highlights the importance of early diagnosis and management of traumatic infections in dogs, identifying bite wounds as entry points for clostridial pathogens and soil or saliva as spore sources. It contributes to understanding the pathogenesis and management of these infections in dogs, emphasizing their poor prognosis and the urgency of addressing clostridial myonecrosis.</p>","PeriodicalId":23690,"journal":{"name":"Veterinary Research Communications","volume":"49 3","pages":"155"},"PeriodicalIF":1.8,"publicationDate":"2025-03-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143735735","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Characterization of African swine fever virus in Angola, 2024.","authors":"Samo Daniel, Ana Paula D Moraes, Irene K Meki, Tirumala B K Settypalli, Charles E Lamien, Gottlieb Aikukutu, Siegfried Khaiseb, William G Dundon, Juliet Kabajani","doi":"10.1007/s11259-025-10721-z","DOIUrl":"https://doi.org/10.1007/s11259-025-10721-z","url":null,"abstract":"<p><p>In February 2024, an outbreak of African Swine Fever (ASF) was reported in a commercial pig farm in southwestern Angola. Five clinical samples were collected and sent to the Central Veterinary Laboratory in Windhoek, Namibia and the presence of ASF virus (ASFV) was confirmed. Sequencing of the C-terminus of the B646L gene (p72 protein), the complete E183L gene (p54 protein), the central hypervariable region (CVR) of the B602L gene, and the partial CD2v gene (used for serotyping) was performed on DNA isolated from the samples. Phylogenetic analyses of the B646L (p72) and the E183L gene (p54) revealed that the samples were identical to each other and belonged to genotype I. The viruses belonged to serotype 2 and had CVR profiles similar to those commonly found in ASFVs of genotype I. This is the first detailed report of ASFV in Angola and will be of interest to regional veterinary authorities.</p>","PeriodicalId":23690,"journal":{"name":"Veterinary Research Communications","volume":"49 3","pages":"154"},"PeriodicalIF":1.8,"publicationDate":"2025-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143711452","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Behavioral and neurological responses of Oreochromis niloticus to chronic cyclic hypoxia: mechanistic insights into hypoxia-responsive genes, pro-inflammatory and apoptotic pathways: role of camel whey protein hydrolysate.","authors":"Amany Abdel-Rahman Mohamed, Abdelwahab A Abdelwarith, Elsayed M Younis, Rowida E Ibrahim, Tarek Khamis, Ali Osman, Mohamed M M Metwally, Abd Elhakeem El-Murr, Simon J Davies, Yasmina M Abd-Elhakim","doi":"10.1007/s11259-025-10678-z","DOIUrl":"https://doi.org/10.1007/s11259-025-10678-z","url":null,"abstract":"<p><p>This study investigates the impact of prolonged cyclic hypoxia on the health of Nile tilapia (Oreochromis niloticus), focusing on behavior, brain oxidative stress, neurotransmitters and inflammatory responses over four weeks, as well as the microarchitectural integrity of brain tissue. Furthermore, the potential protective role of camel whey protein hydrolysate (WPHC, 75 g/kg diet) was examined. Fish were fed a basal diet or a diet replaced with 75 g/kg WPHC, according to the experimental protocol, we assigned four groups; the control (normoxic group), WPHC group (fed the camel whey protein-replaced diet in normoxic conditions), a hypoxia group (cyclic hypoxia), and a hypoxia + WPHC (fed the camel whey protein-replaced diet in hypoxic conditions). The results indicated that cyclic hypoxia significantly impaired ingestive behaviour and swimming activity. Biochemically, it induced oxidative stress, as evidenced by reduced levels of glutathione (GSH), superoxide dismutase (SOD), and catalase (CAT), alongside an increase in malondialdehyde (MDA) in brain tissue. Moreover, the neurotransmitter analysis revealed a marked decline in dopamine (DA) and serotonin (5HT) levels in hypoxic groups compared to normoxic. At the molecular level, there was a notable upregulation of hypoxia-inducible factor alpha (hif-α), fabp-4, pro-inflammatory, and pro-apoptotic genes in response to cyclic hypoxia. Remarkably, fish fed with WPHC-replaced diets showed substantial improvements across behavioral, biochemical, and molecular levels. WPHC also demonstrated a restorative effect on the brain's microarchitecture, mitigating the adverse effects of hypoxia. These findings suggest that WPHC is a valuable alternative protein source in aquaculture to mitigate the neurobehavioural alterations induced by cyclic hypoxia stress, enhancing the accommodation of O. niloticus to this stress through multiple biological pathways. The study underscores the potential of WPHC in promoting sustainable aquaculture practices, particularly in environments disposed to hypoxic stress.</p>","PeriodicalId":23690,"journal":{"name":"Veterinary Research Communications","volume":"49 3","pages":"153"},"PeriodicalIF":1.8,"publicationDate":"2025-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143711450","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Alternative approaches to antibiotics in the control of mastitis in dairy cows: a review.","authors":"Abir Maalaoui, Abdesselem Trimeche, Pierre Guy Marnet","doi":"10.1007/s11259-025-10720-0","DOIUrl":"https://doi.org/10.1007/s11259-025-10720-0","url":null,"abstract":"<p><p>Bovine mastitis is the most widespread and economically burdensome condition affecting dairy herds worldwide, causing substantial financial losses in the livestock and dairy sectors. The main approach to treating mastitis in dairy cows is based on the administration of antibiotics. However, their widespread use has led to the emergence of antibiotic-resistant pathogens, and thus to numerous food safety problems. Consequently, a growing body of scientific research has been directed towards exploring new and effective therapeutic alternatives for the management of bovine mastitis, which could replace conventional antibiotic therapy. This review surveys the various alternative strategies employed in the prevention and treatment of mastitis in dairy cattle. These strategies include nanoparticle therapy, bacteriophage therapy, vaccination, phytotherapy, the use of animal proteins, probiotics and bacteriocins. In addition, the potential synergistic effects resulting from the combination of these treatments has shown real benefits that will be highlighted.</p>","PeriodicalId":23690,"journal":{"name":"Veterinary Research Communications","volume":"49 3","pages":"150"},"PeriodicalIF":1.8,"publicationDate":"2025-03-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143701597","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}