Amany Abdel-Rahman Mohamed, Yasmina M Abd-Elhakim, Nawal Alsubaie, Mohamed M M Metwally, Tarek Khamis, Ali H El-Far, Badriyah S Alotaibi, Samah S Abuzahrah, Rowida E Ibrahim
{"title":"Molecular docking prediction and hepatoprotective potential of genistein-enriched diet against arsenic toxicity in juvenile nile tilapia: implications for ER stress and oxidative damage.","authors":"Amany Abdel-Rahman Mohamed, Yasmina M Abd-Elhakim, Nawal Alsubaie, Mohamed M M Metwally, Tarek Khamis, Ali H El-Far, Badriyah S Alotaibi, Samah S Abuzahrah, Rowida E Ibrahim","doi":"10.1007/s11259-025-10770-4","DOIUrl":"https://doi.org/10.1007/s11259-025-10770-4","url":null,"abstract":"<p><p>The current study evaluated the effects of genistein (GEN) supplementation to alleviate the arsenic (As)-induced hepatotoxicity in Oreochromis niloticus. This was conducted in two steps: a computational prediction study (in silico) and an experimental investigation (in vivo). The prediction step involved molecular docking analysis to assess the interactions between GEN and key stress-related mRNAs in Nile tilapia. In the experimental phase, 160 Nile tilapia fingerlings were randomly assigned to four treatment groups (in four replicates/group) for 60 days: (1) a control group fed a basal diet, (2) a GEN group receiving a GEN-supplemented diet (500 mg/kg), (3) an As group exposed to 10 µg/L As, and (4) an As + GEN group, in which fish was exposed to As and fed the GEN-supplemented diet. The computational assessment of GEN's binding ability revealed strong interactions with key mRNAs associated with inflammation and misfolded protein responses. The in vivo results revealed that GEN significantly alleviated As-induced hepatic oxidative stress and hepatocellular damage by restoring liver enzyme levels, lipid profiles, and bilirubin content and restoring the serum proteins to near-normal values. Additionally, GEN downregulated the expression of endoplasmic reticulum (ER) stress- and inflammation-related genes in the liver tissue of the As + GEN group, compared to the As-exposed fish fed on a basal diet. Additionally, the histopathological analysis further confirmed that GEN supplementation mitigated hepatic tissue damage, reducing necrosis, congestion, and inflammatory cell infiltration. In conclusion, GEN supplementation effectively counteracted As-induced hepatotoxicity in Nile tilapia by modulating oxidative stress, ER stress, and inflammation while preserving liver structure and function. Also, the molecular docking results suggest that GEN interacts with the mRNAs of inflammatory and misfolded protein targets, which are increased due to exposure to As-contaminated water. All our findings highlight GEN as a promising natural dietary additive for improving hepatic health in fish inhabiting As-contaminated environments.</p>","PeriodicalId":23690,"journal":{"name":"Veterinary Research Communications","volume":"49 4","pages":"210"},"PeriodicalIF":1.8,"publicationDate":"2025-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144152033","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Muhammad Umar Javed, Muhammad Ijaz, Arslan Ahmed, Hamza Rasheed, Ali Abdullah Jabir, Maria Batool, Kehkshan Shahid, Ashraf Ali, Muhammad Talha
{"title":"Exploring evolutionary perspectives and antibiogram analysis of biofilm-forming Staphylococcus aureus in goat mastitis.","authors":"Muhammad Umar Javed, Muhammad Ijaz, Arslan Ahmed, Hamza Rasheed, Ali Abdullah Jabir, Maria Batool, Kehkshan Shahid, Ashraf Ali, Muhammad Talha","doi":"10.1007/s11259-025-10776-y","DOIUrl":"https://doi.org/10.1007/s11259-025-10776-y","url":null,"abstract":"<p><p>Biofilm formation plays a key role in developing antimicrobial resistance in Staphylococcus aureus (S. aureus) and is thus a major virulence factor responsible for persistent intramammary infections and subclinical mastitis in goats. The current study investigated the prevalence and molecular characterization of biofilm-forming S. aureus isolated from goat mastitis, the associated risk factors, and comparative antibiogram profiling. A total of 768 milk samples were conveniently collected from farms dairy goats in three districts of Pakistan Multan, Bahawalpur, and Rahim Yar Khan. The results revealed a molecular prevalence (presence of nuc gene) of 206/426 (48.37%) for S. aureus in milk samples from goats. The risk factors analysis showed that age group, water availability, previous mastitis history, hygienic measures during milking, teat lesion, and veterinary services posed significant associations with S. aureus intramammary infections in goats. The prevalence of biofilm production by phenotypic methods i.e. Congo Red agar and Microtitre plate were recorded to be 36.90% and 27.67% respectively. In comparison, the genotypic confirmation was done by the presence of the icaA gene and was found to be 55.34%. The phylogenetic analysis of the icaA gene revealed high homology between sequences of study isolates and the isolates of other countries like India, Egypt, Japan Kenya, the USA, and China. The S. aureus subjected to the Kirby-Bauer disc diffusion method revealed the highest resistance to cefoxitin and oxytetracycline followed by gentamycin, amikacin, trimethoprim + sulphamethoxazole, and vancomycin. There was a slight variation among the resistant profile of biofilm and non-biofilm producing S. aureus strains against antimicrobial agents. Out of 206, 31.07% (64/206) tested isolates exhibited resistance to three or more antibiotics concurrently, categorizing them as multiple drug resistant (MDR) with multiple antibiotic resistance index greater than 0.2. The study concluded that biofilm-producing S. aureus is prevalent in dairy goats of different breeds, including Beetal, Teddi, Barbari, and Makhi-cheeni, across various lactation stages (early, mid, and late), regardless of milk collection times in Pakistan of Pakistan with risk to the udder health and milk quality. Moreover, the biofilm formation may be associated with multidrug resistance of study isolates.</p>","PeriodicalId":23690,"journal":{"name":"Veterinary Research Communications","volume":"49 4","pages":"209"},"PeriodicalIF":1.8,"publicationDate":"2025-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144151999","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Occurrence of mastitis pathogens in cow's milk samples from the Hanover region, north-western Germany - an overview of routine laboratory data from 2005 to 2023.","authors":"Theresa Büthe, Nadja Jessberger, Bettina Schneider, Lothar Kreienbrock, Madeleine Plötz","doi":"10.1007/s11259-025-10773-1","DOIUrl":"10.1007/s11259-025-10773-1","url":null,"abstract":"<p><p>Mastitis remains the most frequent disease in dairy cows, affecting both animal welfare and the profitability of dairy farms. In the present investigation, we retrospectively analysed 102,179 cow's milk samples, which were object of routine diagnostics at the Department of Milk Hygiene, Institute for Food Quality and Food Safety, University of Veterinary Medicine Hannover, from 2005 to 2023, regarding the occurrence of major mastitis pathogens. On average, approx. 48% of all tested samples were bacteriologically positive. Differences in the occurrence of positive findings were described regarding the season, the submitter, the reason for submission, as well as the udder quarters. Generally, the 13 most common groups of pathogens accounted for 94.0% of all positive samples. Non-aureus staphylococci and mammaliicocci (23.6%) were detected most frequently, followed by Streptococcus uberis (14.8%), Escherichia coli (13.2%), Corynebacterium spp. (9.5%), Staphylococcus aureus (6.7%), yeast (6.1%), and Enterococcus spp. (5.6%). While a slight decline was recorded for most of these pathogen groups, S. uberis and E. coli showed a significant upward trend throughout the investigation period. Moreover, a constantly high percentage of samples contaminated with more than three different colony forms was found. Monitoring long-term development of the mastitis pathogen spectrum as well as its antibiotic resistance patterns is of utmost importance to enable adaptation of hygiene and treatment strategies. This includes appropriate training of the treating veterinarians with regard to sterile milk sampling.</p>","PeriodicalId":23690,"journal":{"name":"Veterinary Research Communications","volume":"49 4","pages":"208"},"PeriodicalIF":1.8,"publicationDate":"2025-05-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12098181/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144120976","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Viera Karaffová, Renáta Szabóová, Csilla Tóthová, Rudolf Žitňan, Michaela Čechová, Martin Levkut, Erik Hudec, Zuzana Ševčíková, Monika Röntgen, Elke Albrecht, Róbert Herich
{"title":"Application of Bacillus amyloliquefaciens CECT 5940 influenced muscle satellite cells, PCNA and acute phase protein secretion in broilers.","authors":"Viera Karaffová, Renáta Szabóová, Csilla Tóthová, Rudolf Žitňan, Michaela Čechová, Martin Levkut, Erik Hudec, Zuzana Ševčíková, Monika Röntgen, Elke Albrecht, Róbert Herich","doi":"10.1007/s11259-025-10769-x","DOIUrl":"10.1007/s11259-025-10769-x","url":null,"abstract":"<p><p>Despite the growing interest in the use of probiotics in broilers as feed additives, studies conducted to investigate the effect of probiotic administration on acute phase responses and the impact on muscle growth parameters in broilers are still limited. In this study, we investigated the effect of Bacillus amyloliquefaciens CECT 5940 on relative gene expression for growth factors involved in muscle development (insulin-like growth factors, myogenic factor 5, paired-box transcription factor), percentage of proliferating antigen cell nuclei in breast muscle, and secretion of acute phase proteins (serum amyloid A, haptoglobin, alpha<sub>1</sub>-acid glycoprotein) in the peripheral blood of broilers. Sixty one-day-old chicks from the experimental group were sprayed with a probiotic containing B. amyloliquefaciens at a dose of 1 × 10<sup>10</sup> CFU/g directly after hatching and received the probiotic in drinking water (50 × 10<sup>10</sup> CFU/1000 L) for 5 consecutive days of life. Sampling was performed on the 5th, 8th, and 12th day of life of the chicks. From the obtained results, we can conclude that B. amyloliquefaciens modulated the gene expression of selected growth parameters in the pectoral muscle, thereby increasing the number of satellite cells, however, their uptake into muscle fibers and thus increased hypertrophic growth was not proven at the time of the last sampling. At the same time, it demonstrated a potentiating effect on PCNA expression in chicken breast muscles in the early intensive growth phase of broiler chickens and modulated the production of acute phase proteins, which may contribute to improving adaptive processes during the growth and development of broilers.</p>","PeriodicalId":23690,"journal":{"name":"Veterinary Research Communications","volume":"49 4","pages":"207"},"PeriodicalIF":1.8,"publicationDate":"2025-05-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12095425/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144112132","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
María Florencia Ovelar, Juan Agustín García, Daniel Cook, Dale Gardner, Bryan Stegelmeier, Patricia Diez de Ulzurrun, Agustina Tettamanti, Delfina Balbuena, Elvis Vilatuña Lita, Juan I Poo, María Valeria Scioli, Germán J Cantón
{"title":"Senecio pampeanus poisoning in beef cattle: case report and toxicological evaluation.","authors":"María Florencia Ovelar, Juan Agustín García, Daniel Cook, Dale Gardner, Bryan Stegelmeier, Patricia Diez de Ulzurrun, Agustina Tettamanti, Delfina Balbuena, Elvis Vilatuña Lita, Juan I Poo, María Valeria Scioli, Germán J Cantón","doi":"10.1007/s11259-025-10768-y","DOIUrl":"https://doi.org/10.1007/s11259-025-10768-y","url":null,"abstract":"<p><p>Pyrrolizidine alkaloidosis occurs sporadically in cattle in South America, associated with the consumption of several Senecio species including S. brasiliensis, S. grisebachii, S. madagascariensis, S. oxyphyllus and S. selloi. Although S. pampeanus has been potentially reported as hepatotoxic, there is no scientific evidence of outbreaks associated with its consumption. This report describes a case of chronic hepatotoxicity in a beef heifer in Buenos Aires province in Argentina, grazing native grasslands heavily infested with S. pampeanus. Clinical and pathological findings were consistent with pyrrolizidine alkaloidosis, including hepatic fibrosis, marked hyperplasia of the bile ducts and the presence of abundant hepatomegalocytes. Furthermore, four unsaturated pyrrolizidine alkaloids (PAs) were identified in S. pampeanus including florosenine, doronine, otosenine and desacetyldoronine. Some clinical and histological details are suggestive of a chronic, low-dose pyrrolizidine alkaloidosis probably associated with the low concentration of toxic principles found in S. pampeanus. Further studies are needed to know PAs variation in S. pampeanus and its potential dissemination throughout the region, implying an important risk for livestock.</p>","PeriodicalId":23690,"journal":{"name":"Veterinary Research Communications","volume":"49 4","pages":"205"},"PeriodicalIF":1.8,"publicationDate":"2025-05-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144112158","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Guilherme Ramon Vieira da Silva, Luiz Emanuel Campos Francelino, Samuel Ferreira de França Silvestre, Maria Danielle Chagas Dos Santos, Francisco Joelson Correia de Freitas, Ana Caroline Freitas Caetano de Sousa, Clodomiro Alves Júnior, Moacir Franco de Oliveira, Carlos Iberê Alves Freitas, Carlos Eduardo Bezerra de Moura
{"title":"Use of cold atmospheric plasma (CAP) for the control of gingival inflammation in a rabbit (Oryctolagus cuniculus) with progressive syndrome of acquired dental disease (PSADD): case report.","authors":"Guilherme Ramon Vieira da Silva, Luiz Emanuel Campos Francelino, Samuel Ferreira de França Silvestre, Maria Danielle Chagas Dos Santos, Francisco Joelson Correia de Freitas, Ana Caroline Freitas Caetano de Sousa, Clodomiro Alves Júnior, Moacir Franco de Oliveira, Carlos Iberê Alves Freitas, Carlos Eduardo Bezerra de Moura","doi":"10.1007/s11259-025-10775-z","DOIUrl":"https://doi.org/10.1007/s11259-025-10775-z","url":null,"abstract":"<p><p>Progressive syndrome of acquired dental disease (PSADD) is common in domestic rabbits and is often associated with chronic gingivitis, which compromises quality of life. Conventional treatment involves prolonged use of antimicrobials and anti-inflammatories, which may cause resistance and adverse effects. Cold atmospheric plasma (CAP) has antimicrobial, anti-inflammatory and regenerative properties, suggesting its therapeutic potential. This pilot study evaluated the effects of CAP as a complementary treatment for gingivitis associated with PSADD in a domestic rabbit. Two applications of the plasma jet were performed on the gingiva of the incisors, with an interval of seven days. The CAP was generated with helium gas at 2 L/min, 1.2 W power, 1.08 mA current and 10 Hz frequency, with application time of 30s/cm² of the affected area. Efficacy was assessed by clinical and histopathological scores before and after the intervention. The results indicated a significant reduction in gingival hyperemia and edema, in addition to the inhibition of the inflammatory infiltrate and regeneration of the lamina propria. After treatment, the patient also presented weight gain of 100 g, suggesting improvement in general well-being. These findings reinforce the potential of CAP as a complementary approach in PSADD, reducing the need for prolonged pharmacological therapies and promoting tissue regeneration.</p>","PeriodicalId":23690,"journal":{"name":"Veterinary Research Communications","volume":"49 4","pages":"206"},"PeriodicalIF":1.8,"publicationDate":"2025-05-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144112167","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Álex Gómez, Sergio Villanueva-Saz, Antonio Fernández, Estela Pérez, Lluís Luján, Diego Cortés
{"title":"Tiamulin and monensin intoxication in commercial brown pullets.","authors":"Álex Gómez, Sergio Villanueva-Saz, Antonio Fernández, Estela Pérez, Lluís Luján, Diego Cortés","doi":"10.1007/s11259-025-10771-3","DOIUrl":"10.1007/s11259-025-10771-3","url":null,"abstract":"<p><p>Tiamulin and monensin intoxication has been widely reported in broilers and many other domestic animals, causing muscular dystrophy. This study reports an outbreak of subacute tiamulin-monensin toxicosis in commercial brown pullets and provides a detailed clinicopathological description. The outbreak occurred on a floor-rearing farm in Spain of 21,044 Lohmann Brown pullets (11-16 weeks old) after monensin (120 ppm) was administered in feed and tiamulin (150 ppm) in drinking water. Within 24 h after tiamulin treatment, feed and water consumption declined drastically, and mortality increased progressively, reaching a peak of 1.75%. Cumulative mortality over a 13-day period was 4.3%. Affected pullets (8%) exhibited marked apathy, muscle weakness, flaccid paralysis, and prostration. Necropsy of 18 naturally dead pullets revealed gross myocardial (72%) and vastus intermedius (100%) muscle pallor, with histopathological examination confirming monophasic myocardial and myocyte degeneration and necrosis (100%). Fibrosis and granulomatous infiltration surrounding and infiltrating affected muscle fibers were observed in a low number of animals (11%). These findings suggest that tiamulin enhances monensin toxicity through biochemical interactions, indicating that the combination of 150 ppm tiamulin and 120 ppm monensin is highly toxic to pullets. This highlights the need for careful administration in poultry production. Twenty-four hours after tiamulin-monensin withdrawal, feed and water consumption increased and mortality decreased. Tiamulin-monensin toxicosis should be considered in the differential diagnosis of cardiomuscular disorders in pullets and layers.</p>","PeriodicalId":23690,"journal":{"name":"Veterinary Research Communications","volume":"49 4","pages":"203"},"PeriodicalIF":1.8,"publicationDate":"2025-05-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12092491/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144112162","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Immunohistochemistry and molecular biology studies of apelin and apelin receptor in queen placenta.","authors":"Sara Pastore, Cecilia Dall'Aglio, Margherita Maranesi, Guillaume Robiteau, Viola Zappone, Tiziana Caspanello, Alain Fontbonne, Angela Polisca, Andrea Verini Supplizi, Alessandro Troisi","doi":"10.1007/s11259-025-10766-0","DOIUrl":"10.1007/s11259-025-10766-0","url":null,"abstract":"<p><p>Placenta is a tissue where vasculogenesis, blood pressure and blood flow are dramatically important to allow normal embryonic and foetal growth and requires the production of numerous growth factors, hormones and transcription factors. Apelin is a pleiotropic peptide, and its major action relates to energy metabolism, cardiovascular function, body fluid homeostasis via its receptor. The involvement of the apelinergic system during pregnancy in veterinary medicine has been investigated only in bitches. Thereafter, the aim of our study was to investigate, for the first time, presence and distribution of this system in the queen placenta at mid- and end-gestation. Ten pregnant mixed-breed queens were used. The animals were equally divided into two groups based on the stage of pregnancy (mid and end gestation) and, with the written consent of their owners, were subjected to ovariohysterectomy or non-conservative caesarean section. The Real-Time PCR (RT-PCR) analysis showed the presence of transcripts for apelin and its receptor in all the foetal and maternal placenta samples processed. The immunohistochemical (IHC) study evidenced the presence and the distribution of positive immunoreactions for apelin and its receptor in all the samples observed. In particular, in the placental labyrinthic portion, apelin and apelin receptor immunopositivity was evident in the cytoplasm of trophoblasts and endothelial cells. The uterine glands also exhibited a positive immune reaction for apelin and corresponding receptor. Based on our results, apelin and its receptor, also in the queen placenta, could be an important system involved in the physiological development of placenta, embryo and foetal growth.</p>","PeriodicalId":23690,"journal":{"name":"Veterinary Research Communications","volume":"49 4","pages":"204"},"PeriodicalIF":1.8,"publicationDate":"2025-05-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12092554/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144112155","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Monoclonal antibody based competitive 3AB non-structural protein ELISA for detection of foot-and-mouth disease virus infection.","authors":"Smrutirekha Mallick, Jajati Keshari Mohapatra, Jitendra Kumar Biswal, Manoranjan Rout, Rajeev Ranjan, Reshama Samanta, Rabindra Prasad Singh","doi":"10.1007/s11259-025-10767-z","DOIUrl":"https://doi.org/10.1007/s11259-025-10767-z","url":null,"abstract":"<p><p>Foot and mouth disease (FMD) is one of the most infectious viral diseases of cloven-footed animals which cause devastating global economic losses. FMD virus (FMDV) specific non-structural protein (NSP) antibodies are indicative of a current or past infection with any of the seven FMDV serotypes(O, A, C, Asia1, SAT1, SAT2, SAT3). In the present study, a monoclonal antibody (MAb) was developed against recombinant FMDV NSP 3AB3 (r3AB3) via hybridoma system and applied in a competitive enzyme linked immunosorbent assay (cELISA) for NSP antibody detection. The newly generated MAb #FMDV3AB-2E4 was identified as an IgG1 isotype that specifically binds to NSP 3B. A cELISA termed FMDV3AB-2E4 cELISA was developed using r3AB3 as the antigen and the MAb. Percent inhibition (PI) cut-off of the cELISA was determined by receiver operating characteristics curve analysis of 202 known FMDV-positive and 730 negative serum samples from multiple species (cattle, buffalo sheep, goat, pig and deer). At the selected cut-off value of 45 PI the cELISA exhibited diagnostic sensitivity (DSn) of 95.54% and diagnostic specificity (DSp) of 98.36% at 95% confidence interval. Compared to commercial PrioCHECK FMD NS test the DSn was 93.54% and DSp was 97.46% for bovine and caprine sera (n = 446) obtained from naïve, FMDV-infected and vaccinated animals. Concordance rate of new cELISA and extensively used in-house r3AB3 indirect ELISA was 96.61% for test results of 3016 cattle sera collected across multi-locations in India. The species independent NSP antibody test developed would be a useful tool in FMD control efforts in India and other countries.</p>","PeriodicalId":23690,"journal":{"name":"Veterinary Research Communications","volume":"49 4","pages":"200"},"PeriodicalIF":1.8,"publicationDate":"2025-05-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144086774","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Flávia Fiori, Rogério Cunha de Paula, Ricardo Luiz Pires Boulhosa, Ricardo Augusto Dias
{"title":"Clinical evaluation of sarcoptic mange (Sarcoptes scabiei) in maned wolves (Chrysocyon brachyurus).","authors":"Flávia Fiori, Rogério Cunha de Paula, Ricardo Luiz Pires Boulhosa, Ricardo Augusto Dias","doi":"10.1007/s11259-025-10774-0","DOIUrl":"https://doi.org/10.1007/s11259-025-10774-0","url":null,"abstract":"<p><p>Sarcoptic mange is a disease potentially threatening to Brazilian carnivores, especially maned wolves (Chrysocyon brachyurus). Recently, an outbreak of sarcoptic mange has affected maned wolves in part of their range; therefore, it is a priority to assess the clinical manifestation of the disease. A total of 20 maned wolves were evaluated in Southeast Brazil, 12 of which from research projects and eight were rescued between 2008 and 2023. Since the clinical signs of the sarcoptic mange vary considerably, the maned wolves were classified according to the degree of clinical manifestation and the lesion severity. According to the extension of the body affected by sarcoptic mange, two maned wolves (10%) were classified as level I (< 25% of the body affected), five (25%) as level II (26-50% of the body affected), three (15%) as level III (51-75% of the body affected), and ten maned wolves (50%) as level IV (76-100% of the body affected). By lesion severity, five maned wolves (25%) had type A lesions (mild lesions), ten (50%) had type B lesions, and five (25%) had type C lesions (severe lesions). The maned wolves classified as III-C and IV-C were transferred to captivity to receive intensive veterinary care. The definitive diagnosis was challenging due to the low sensitivity of the available diagnostic tests, suggesting that future research should use a diagnostic protocol that combines blood serum antibody testing with skin scrapings and histopathology.</p>","PeriodicalId":23690,"journal":{"name":"Veterinary Research Communications","volume":"49 4","pages":"202"},"PeriodicalIF":1.8,"publicationDate":"2025-05-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144086768","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}