Veterinary Research Communications最新文献_第2页

Prevalence rates of Babesia caballi and Theileria equi in the horse population of northern Spain: a serological and molecular study. 西班牙北部马群中卡巴贝斯虫和马尾丝虫的流行率：血清学和分子研究。

IF 1.8 3区农林科学

Veterinary Research Communications Pub Date : 2025-03-24 DOI: 10.1007/s11259-025-10722-y

María Paz Peris, María Serrano, Antonio Romero, Mirta García, Nabil Halaihel, Juan Antonio Castillo, María Jesús Gracia

{"title":"Prevalence rates of Babesia caballi and Theileria equi in the horse population of northern Spain: a serological and molecular study.","authors":"María Paz Peris, María Serrano, Antonio Romero, Mirta García, Nabil Halaihel, Juan Antonio Castillo, María Jesús Gracia","doi":"10.1007/s11259-025-10722-y","DOIUrl":"https://doi.org/10.1007/s11259-025-10722-y","url":null,"abstract":"Equine piroplasmosis (EP), a tick-borne disease caused by Babesia caballi and Theileria equi, is of significant concern due to its impact on the international horse trade. According to standards established by the World Organisation for Animal Health (WOAH), horses imported from EP-endemic regions must have a certificate confirming negative serological and molecular test results. In EP-free countries, only seronegative horses are allowed to enter. This entails economic losses for endemic regions such as Spain. Our study aimed to assess the prevalence of B. caballi and T. equi in horses from northern Spain and to compare indirect fluorescent antibody tests (IFAT) with polymerase chain reaction (PCR) regarding diagnostic accuracy. In March 2019, blood samples were collected from 50 horses at a slaughterhouse in Zaragoza. The horses originated from meat farms in the Pyrenees and Cantabrian coastal regions, including northern Cantabria, the Basque Country, and Navarre. Serological results revealed a prevalence of 50% for B. caballi, 30% for T. equi, and 16% for mixed infections, with an overall EP prevalence of 64%. PCR results confirmed 22% positive for B. caballi, 42% for T. equi, and 16% for both parasites in one animal, showing an overall EP prevalence of 54%. Our study underscores the critical need for combining serological and molecular diagnostic tests to accurately identify carriers and active infections, with the goal of ensuring safe animal movement and controlling EP transmission.","PeriodicalId":23690,"journal":{"name":"Veterinary Research Communications","volume":"49 3","pages":"151"},"PeriodicalIF":1.8,"publicationDate":"2025-03-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143701627","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Dietary Pelargonium Sidoides extract mitigates thermal stress in Oreochromis niloticus: physiological and immunological insights.

IF 1.8 3区农林科学

Veterinary Research Communications Pub Date : 2025-03-24 DOI: 10.1007/s11259-025-10705-z

Nagwa I S Abu-Zahra, Ayman A Atia, Mohamed M Elseify, Mona E Abass, Shireen Soliman

{"title":"Dietary Pelargonium Sidoides extract mitigates thermal stress in Oreochromis niloticus: physiological and immunological insights.","authors":"Nagwa I S Abu-Zahra, Ayman A Atia, Mohamed M Elseify, Mona E Abass, Shireen Soliman","doi":"10.1007/s11259-025-10705-z","DOIUrl":"10.1007/s11259-025-10705-z","url":null,"abstract":"Extreme water temperatures caused by climate change constitute a serious threat to aquaculture producers. Aquatic ectotherms, which are unable to regulate their body temperature, are highly affected by temperature changes and serve as excellent models for studying the effects of these factors. This study aimed to examine the impact of extreme temperature variation on the growth, biological indices, antioxidant capacity, histology, and disease resistance of Oreochromis niloticus. The fish were exposed to 17 °C, 25 °C (control), or 33 °C for 30 days, imitating extreme temperature events. Additionally, a mitigation trial was conducted via dietary Pelargonium sidoides root extract (PS). Compared with that at 25 °C, growth performance was significantly impaired by thermal stress. The exposure of O. niloticus to thermal stress, especially cold stress, elicited significant increases in stress biomarkers (cortisol and glucose), hepatorenal functions (AST, ALT, LDH, and creatinine), and lipid profiles (triglyceride, cholesterol, HDL, and LDL). Cold stress induced immunosuppression in terms of reduced total protein, phagocytosis, serum bacterial activity, total immunoglobulin, and white blood cells, all of which increased the susceptibility of the fish to Aeromonas hydrophila infection and increased mortality. In addition to severe pathological lesions in the gills, liver, and intestine, thermal stress causes an imbalance in antioxidant/oxidative stress. Nevertheless, the PS extract counteracted these detrimental impacts of thermal stress. In conclusion, PS may be recommended for enhancing immunity and antioxidative resistance to mitigate the effects of thermal stress on O. niloticus. These findings may be useful in developing hypotheses about the physiology and immunology of fish in extreme-temperature environments.","PeriodicalId":23690,"journal":{"name":"Veterinary Research Communications","volume":"49 3","pages":"152"},"PeriodicalIF":1.8,"publicationDate":"2025-03-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11933177/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143701621","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Isolation and biological characterization of a variant pseudorabies virus strain from goats in Yunnan Province, China.

IF 1.8 3区农林科学

Veterinary Research Communications Pub Date : 2025-03-22 DOI: 10.1007/s11259-025-10703-1

Yuqing Duan, Ning Liu, Lei Tan, Lin Gao, Gangyi Zhu, Zhengze Tao, Baoyou Liu, Aibing Wang, Jun Yao

{"title":"Isolation and biological characterization of a variant pseudorabies virus strain from goats in Yunnan Province, China.","authors":"Yuqing Duan, Ning Liu, Lei Tan, Lin Gao, Gangyi Zhu, Zhengze Tao, Baoyou Liu, Aibing Wang, Jun Yao","doi":"10.1007/s11259-025-10703-1","DOIUrl":"https://doi.org/10.1007/s11259-025-10703-1","url":null,"abstract":"Pseudorabies virus (PRV), the causative agent of Aujeszky's disease, is an infectious pathogen that significantly impacts the global swine industry. The broad host range of PRV enables it to infect various animals, including pigs, cattle, minks, dogs, and even humans. Although PRV infections in ruminants have been reported, the occurrence of natural PRV infection in goats has not been documented. In this study, we present compelling evidence through the characterization of clinical symptoms and detection and analysis using polymerase chain reaction, enzyme-linked immunosorbent assay, and sequence alignment, demonstrating the first case of a PRV variant infection that led to acute illness and death in goats in Yunnan Province, China. Our results indicated that all 10 serum samples from fattening pigs raised near the infected goats tested positive for PRV, suggesting that the PRV infection in goats may have resulted from shared feeding with PRV-infected fattening pigs. Consequently, this report not only underscores the potential threat posed by newly emerging PRV variant strains to the goat industry but also emphasizes the necessity for the development of effective and safe vaccines against PRV variants for goats and other ruminants in the future.","PeriodicalId":23690,"journal":{"name":"Veterinary Research Communications","volume":"49 3","pages":"149"},"PeriodicalIF":1.8,"publicationDate":"2025-03-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143693583","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Tissue distribution of Ocimum basilicum essential oil in Nile tilapia Oreochromis niloticus after anesthesia. 麻醉后尼罗罗非鱼体内欧鼠李精油的组织分布。

IF 1.8 3区农林科学

Veterinary Research Communications Pub Date : 2025-03-21 DOI: 10.1007/s11259-025-10716-w

Emilly Monteiro Lopes, Arlene Sobrinho Ventura, Claudia Andrea Lima Cardoso, Alexandre Vaz da Silva, Caio Francisco Santana Farias, Domickson Silva Costa, Ana Paula de Souza, Danilo Vitor Vilhena Batista, Silvia Terra Fontes, Gabriela Tomas Jerônimo, José Luiz Pedreira Mouriño, Maurício Laterça Martins

{"title":"Tissue distribution of Ocimum basilicum essential oil in Nile tilapia Oreochromis niloticus after anesthesia.","authors":"Emilly Monteiro Lopes, Arlene Sobrinho Ventura, Claudia Andrea Lima Cardoso, Alexandre Vaz da Silva, Caio Francisco Santana Farias, Domickson Silva Costa, Ana Paula de Souza, Danilo Vitor Vilhena Batista, Silvia Terra Fontes, Gabriela Tomas Jerônimo, José Luiz Pedreira Mouriño, Maurício Laterça Martins","doi":"10.1007/s11259-025-10716-w","DOIUrl":"https://doi.org/10.1007/s11259-025-10716-w","url":null,"abstract":"This study evaluated how the levels of methyl chavicol and linalool behave in tissues of juvenile Nile tilapia after anesthesia with the essential oil (EO) of Ocimum basilicum at different temperatures. The EO was diluted in ethanol (1:9), and the fish (64.99 ± 13.90 g) were immersed in the anesthetic solution (250 mg L-1) at 23 and 27 ºC for 10 min. After anesthesia, the fish were placed in tanks free of anesthetic solution for recovery. Tissues (gill, brain, liver and muscle) were collected at 0, 0.5, 3, 6, and 12 h after anesthesia. The compounds were quantified in the gills, brain and liver up to 6 h, and in the muscle up to 12 h at both temperatures. The maximum concentration (Cmax) of both compounds was highest in the liver at 23 ºC and lowest in the muscle at 27 ºC. In the gills, the Cmax of methyl chavicol and linalool was highest at 23 ºC with half-lives of 0.78 h and 0.70 h, respectively. The levels of methyl chavicol and linalool in the brain were similar at 23 and 27 ºC. In the liver, elimination of the compounds was more efficient at 27 ºC, with a significant interaction between temperature and depletion. In the muscle, the compounds showed greater retention, with slower elimination compared to the other tissues. The temperature of 23 ºC favored greater absorption of the compounds in the gills and liver, while 27 ºC accelerated their elimination. The brain and muscle, on the other hand, were not influenced by temperature. The liver was identified as the main organ for metabolizing the compounds, with less elimination in the muscle. The results highlight the impact of temperature on the metabolism of anesthetics in fish.","PeriodicalId":23690,"journal":{"name":"Veterinary Research Communications","volume":"49 3","pages":"148"},"PeriodicalIF":1.8,"publicationDate":"2025-03-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143671084","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Dietary methanol extract of purslane (Portulaca oleracea L.) can help reduce the negative impacts of triiodothyronine-induced ascites in broiler chickens. 马齿苋甲醇提取物有助于减少三碘甲状腺原氨酸诱发肉鸡腹水的负面影响。

IF 1.8 3区农林科学

Veterinary Research Communications Pub Date : 2025-03-20 DOI: 10.1007/s11259-025-10723-x

Mahmood Habibian, Amirali Sadeghi, Ahmad Karimi

{"title":"Dietary methanol extract of purslane (Portulaca oleracea L.) can help reduce the negative impacts of triiodothyronine-induced ascites in broiler chickens.","authors":"Mahmood Habibian, Amirali Sadeghi, Ahmad Karimi","doi":"10.1007/s11259-025-10723-x","DOIUrl":"https://doi.org/10.1007/s11259-025-10723-x","url":null,"abstract":"This study investigated the effects of purslane methanol extract (PME) on broilers suffering from triiodothyronine (T3)-induced ascites. A total of 240 one-day-old chicks were divided into four dietary treatment groups: a control diet, a T3 diet (1.5 mg/kg), a T3 diet added with 150 mg/kg PME, and a T3 diet added with 300 mg/kg PME. Results indicated that the T3-treated birds exhibited a higher right ventricle to total ventricle (RV/TV) ratio and increased mortality due to ascites, alongside reduced feed intake, body weight gain, and production efficiency index, while showing a higher feed conversion ratio compared with controls. Despite the lack of significant enhancement in growth performance due to dietary PME supplementation, the group that was administered a diet with 300 mg/kg PME demonstrated an improved production efficiency index, as well as decreased mortality and a reduced RV/TV ratio. Additionally, the birds subjected to T3 treatment displayed diminished activity of antioxidant enzymes (superoxide dismutase, catalase, and glutathione peroxidase), in conjunction with elevated malondialdehyde levels compared with the control birds. Supplementation with 300 mg/kg PME increased antioxidant enzyme activities and lowered malondialdehyde concentrations to near control levels. Furthermore, T3 treatment led to elevated red blood cell counts, hematocrit, hemoglobin, and heterophil to lymphocyte ratio, which were significantly mitigated by PME supplementation. To conclude, PME effectively mitigates the adverse effects of T3-induced ascites in broilers by enhancing antioxidant enzyme activity and improving production efficiency, suggesting its potential as a dietary supplement in poultry production.","PeriodicalId":23690,"journal":{"name":"Veterinary Research Communications","volume":"49 3","pages":"147"},"PeriodicalIF":1.8,"publicationDate":"2025-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143671083","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

First report of Moraxella oculi in Brazil in an infectious bovine keratoconjunctivitis outbreak.

IF 1.8 3区农林科学

Veterinary Research Communications Pub Date : 2025-03-19 DOI: 10.1007/s11259-025-10713-z

Clarissa Vidal de Carvalho, Robert Domingues, Cinthia de Carvalho Coutinho, Nicole Tafnes de Brito Silva Honório, Daniele Ribeiro de Lima Reis Faza, Alessandra Barbosa Ferreira-Machado, Wanessa Araújo Carvalho, Emanuelle Baldo Gaspar, Marta Fonseca Martins

{"title":"First report of Moraxella oculi in Brazil in an infectious bovine keratoconjunctivitis outbreak.","authors":"Clarissa Vidal de Carvalho, Robert Domingues, Cinthia de Carvalho Coutinho, Nicole Tafnes de Brito Silva Honório, Daniele Ribeiro de Lima Reis Faza, Alessandra Barbosa Ferreira-Machado, Wanessa Araújo Carvalho, Emanuelle Baldo Gaspar, Marta Fonseca Martins","doi":"10.1007/s11259-025-10713-z","DOIUrl":"https://doi.org/10.1007/s11259-025-10713-z","url":null,"abstract":"Infectious Bovine Keratoconjunctivitis (IBK) is a widespread ocular disease that affects dairy and beef cattle worldwide, caused by Gram-negative bacteria from the genus Moraxella. It is the most common eye disease in cattle, with symptoms including tearing, ocular pain, corneal opacity, photophobia, ulceration, and, in severe cases, permanent blindness. This study focused on characterizing Moraxella species in a 2022 IBK outbreak in Minas Gerais, Brazil. Ocular swabs from 18 symptomatic Holstein cattle were analyzed through colony isolation, physiological tests, and molecular techniques, including PCR-RFLP and sequencing. Results revealed one isolate of Moraxella bovoculi genotype 1 and five isolates of Moraxella oculi, marking the first report of the latter species in Brazil. This study represents the second report of Moraxella oculi isolation and the first in a country different from the initial report. Notably, Moraxella bovis was not isolated in this outbreak. All isolates exhibited susceptibility to the tested antibiotics. Comparative genomic analysis between the Brazilian Moraxella oculi isolate and the American strain (Tifton 1) revealed 99.5% similarity in the 16-23 S rRNA locus and 99.0% average nucleotide identity.","PeriodicalId":23690,"journal":{"name":"Veterinary Research Communications","volume":"49 3","pages":"143"},"PeriodicalIF":1.8,"publicationDate":"2025-03-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143664635","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

First identification and molecular characterization of protozoan parasites associated with abortion in ruminants from South Sinai Governorate, Egypt.

IF 1.8 3区农林科学

Veterinary Research Communications Pub Date : 2025-03-19 DOI: 10.1007/s11259-025-10698-9

Safaa Mohamed Barghash, Al-Shaimaa Mohsen Sadek

{"title":"First identification and molecular characterization of protozoan parasites associated with abortion in ruminants from South Sinai Governorate, Egypt.","authors":"Safaa Mohamed Barghash, Al-Shaimaa Mohsen Sadek","doi":"10.1007/s11259-025-10698-9","DOIUrl":"10.1007/s11259-025-10698-9","url":null,"abstract":"This study presents the first molecular detection and characterization of protozoa associated with livestock abortion, including Toxoplasma gondii, Neospora caninum, Sarcocystis spp., and Microsporidia spp., in the South Sinai Governorate, Egypt. A total of 226 blood samples were collected from cattle, goats, and sheep with a history of abortion. Molecular analysis was conducted using PCR and sequencing of the internal transcribed spacer (ITS) region of the rRNA gene. The results revealed the prevalence of T. gondii in cattle at 12.33% (9/73), while goats and sheep tested negative. In contrast, 14.92% (10/67) of goat samples tested positive for Microsporidia spp., with no cases detected in cattle or sheep. No infections with Sarcocystis spp. or N. caninum were identified in any tested animals. Across all examined livestock, the overall prevalence of T. gondii was 3.98% (9/226), while Microsporidium spp. exhibited a slightly higher prevalence at 4.42% (10/226). Molecular sequencing identified three novel T. gondii strains in cattle recorded in the GenBank database under accession numbers MZ197902, MZ197903, and MZ197904, showing 99.9% genetic similarity to reference strains. Additionally, three novel strains of Enterocytozoon bieneusi (MZ197781, MZ197782, and MZ197783) were detected in goats. Phylogenetic analysis indicated a close evolutionary relationship between these newly identified E. bieneusi strains and known genotypes, suggesting a high degree of genetic conservation. These findings underscore the ongoing risks of T. gondii and E. bieneusi to livestock and public health. Continued surveillance is necessary to assess the zoonotic potential of these novel strains to aid in effective management strategies.","PeriodicalId":23690,"journal":{"name":"Veterinary Research Communications","volume":"49 3","pages":"145"},"PeriodicalIF":1.8,"publicationDate":"2025-03-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11922995/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143664633","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Fecal strains Enterococcus mundtii from wild ruminants, their safety and postbiotic potential.

IF 1.8 3区农林科学

Veterinary Research Communications Pub Date : 2025-03-19 DOI: 10.1007/s11259-025-10701-3

Andrea Lauková, Valentína Focková, Marián Maďar, Grzegorz Belzecki, Renata Miltko, Monika Pogány Simonová

{"title":"Fecal strains Enterococcus mundtii from wild ruminants, their safety and postbiotic potential.","authors":"Andrea Lauková, Valentína Focková, Marián Maďar, Grzegorz Belzecki, Renata Miltko, Monika Pogány Simonová","doi":"10.1007/s11259-025-10701-3","DOIUrl":"10.1007/s11259-025-10701-3","url":null,"abstract":"Roe and red deers represent inhabitants in European forests but also in agricultural areas. In general, microbiota can have a significant impact on health. E. g. the genus Enterococcus was detected in more or less high abundance percentage in feces of red deers. To preserve negative impact of some microbiota, beneficial autochthonous strains can be selected for this purpose. The aim of this study was to assess safety, postbiotic activity and character of the fecal strains Enterococcus mundtii from roe and red deers living in Poland to spread basic microbiology research in this field and to select application candidate. Taxonomy of seven E. mundtii was determined using MALDI-TOF mass spectrometry and 16S rRNA sequence analysis. The evaluation score responded mostly with secure genus identification/probable species identification. Strains showed identity up to 100% with the sequence MK414812.1 of E. mundtii in GenBank. They were deoxyribonuclease and gelatinase- negative, with low-grade biofilm formation (0.100 ± 0.31 to 0.181 ± 0.43). E. mundtii were susceptible to antibiotics without production of damaging enzymes. They were absent of virulence factors genes (gelE, agg, esp, efaAfm, efaAfs). Postbiotic potential of the bacteriocin substance produced by E. mundtii revealed inhibition of indicator strains growth up to 48%. The most active substance was from the strain EM 1/90/2; inhibitory activity against enterococci, staphylococci and listeriae up to 86% (up to 1600 AU mL) was noted. The substance from the strain EM 6/123/1 reached inhibitory activity up to 81% with activity up to 400 AU/mL. It remained active at -20 ˚C for one month. E. mundtii were assessed with no and/or low- grade virulence factor rate and with postbiotic potential for further studies.","PeriodicalId":23690,"journal":{"name":"Veterinary Research Communications","volume":"49 3","pages":"141"},"PeriodicalIF":1.8,"publicationDate":"2025-03-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11923013/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143664620","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Total knee replacement in a dog with an articular epiphyseal medial condylar fracture of the distal femur, a case report.

IF 1.8 3区农林科学

Veterinary Research Communications Pub Date : 2025-03-19 DOI: 10.1007/s11259-025-10712-0

Brent R Fink, Laurent P Guiot, Peter Muir

引用次数: 0

Detection and genomic characterization of foot and mouth disease virus SAT2 XIV topotype using amplicon-based nanopore sequencing.

IF 1.8 3区农林科学

Veterinary Research Communications Pub Date : 2025-03-19 DOI: 10.1007/s11259-025-10718-8

Ibrahim H M Abualghusein, Mustafa M K Ababneh, Mohammad Borhan F Al-Zghoul, Daoud A A Alghizzawi, Hebah A A Aboomer

{"title":"Detection and genomic characterization of foot and mouth disease virus SAT2 XIV topotype using amplicon-based nanopore sequencing.","authors":"Ibrahim H M Abualghusein, Mustafa M K Ababneh, Mohammad Borhan F Al-Zghoul, Daoud A A Alghizzawi, Hebah A A Aboomer","doi":"10.1007/s11259-025-10718-8","DOIUrl":"https://doi.org/10.1007/s11259-025-10718-8","url":null,"abstract":"Foot and mouth disease (FMD) is a highly contagious vesicular viral disease that infects cloven-hoofed animals. This disease is caused by Foot-and-mouth disease virus (FMDV) which is composed of seven serotypes (O, A, C, Asia-1, SAT1, SAT2, and SAT3), each of which lacks cross immune protection among serotypes which complicates vaccination strategies. For that, sequencing of viral protein 1 (VP1) is crucial to identify circulating serotypes in a country. Jordan is endemic for FMD, with primarily O and A serotypes circulating according to previous data. However, the epidemiological status of FMD requires updates in Jordan. In this study, out of 200 samples collected during a recent outbreak in Al-Dhlail, Jordan in early 2023, forty-nine were positive by quantitative polymerase chain reaction (qPCR). Fourteen of these positive samples were selected for PCR amplification of the viral protein 1 (VP1) gene, which were then sequenced using nanopore technology. Phylogenetic analysis highlighted the presence of SAT2 XIV topotype, with eleven samples successfully sequenced using this approach. In addition to that, Sanger sequencing validated a N137S SNP in the VP1 protein that initially identified by our approach. This study provides additional data that can be used and incorporated into the national and global efforts against this disease with new means of diagnostics methods.","PeriodicalId":23690,"journal":{"name":"Veterinary Research Communications","volume":"49 3","pages":"144"},"PeriodicalIF":1.8,"publicationDate":"2025-03-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143664597","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0