Veterinary Research Communications最新文献

{"title":"Potential antioxidant and anti-inflammatory impacts of Salvia officinalis leaves extract on mice experimentally infected with Trichinella spiralis.","authors":"Marwa Safi-Eldin, Mahmoud M Abdelfattah, Safaa Barghash, Ameen A Ashour, Hoda A Taha","doi":"10.1007/s11259-025-10875-w","DOIUrl":"10.1007/s11259-025-10875-w","url":null,"abstract":"<p><p>Trichinellosis is a dangerous food-borne zoonotic infection with a global spread. Treatment is currently insufficient. The study aimed to assess the potential therapeutic effects of Sage (Salvia officinalis) leaves extract in comparison to albendazole against Trichinella spiralis infection in mice. Seventy mice were classified into six groups, non-infected control, non-infected receiving Salvia officinalis extract, infected control, infected and treated with albendazole (50 mg/kg), infected and received Salvia officinalis extract (250 mg/kg), and infected and treated with combination of 25 mg/kg albendazole and 250 mg/kg S. officinalis extract. Mice were sacrificed at 7- and 37-days post-infection for intestinal and muscle phases, respectively. Efficacy was assessed through parasitological parameters, anti-inflammatory and oxidative stress markers. Our finding revealed a significant reduction in the adult worms and larvae counts of T. spiralis in albendazole (97.3% (P < 0.01), 74.19% (P < 0.05), S. officinalis extract (80% (P < 0.05), 60% (P < 0.05) and combined treatment led to the highest reduction rate (91.9% (P < 0.05), 81.4% (P < 0.01). S. officinalis extract boosted the immune response by reducing NO and MDA levels while increasing SOD, CAT and GSH in intestinal and muscular tissues. Additionally, it improved the inflammatory response, Albendazole and S. officinalis, especially in combination, have effectively suppress inflammatory and fibrotic gene expression (FN1, TNFα, TGF-β, and IL-10) indicating synergistic therapeutic efficacy. In conclusion, S. officinalis extract can be considered a practical and effective alternative herbal drug in treating trichinellosis.</p>","PeriodicalId":23690,"journal":{"name":"Veterinary Research Communications","volume":"49 6","pages":"337"},"PeriodicalIF":2.0,"publicationDate":"2025-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12484373/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145201802","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pharmacokinetics of praziquantel in rainbow trout, Oncorhynchus mykiss (Walbaum 1792) following a single dose oral administration.","authors":"Sumanta Kumar Mallik, Richa Pathak, Krishna Kala, Prasanna Kumar Patil, Neetu Shahi, Ritesh Shantilal Tandel, Niladri Sekhar Chatterjee, Ranjit Kumar Nadella, Kishor Kunal","doi":"10.1007/s11259-025-10895-6","DOIUrl":"https://doi.org/10.1007/s11259-025-10895-6","url":null,"abstract":"<p><p>This study examined the pharmacokinetics of praziquantel (PZQ) in rainbow trout (Oncorhynchus mykiss) following a single oral dose of 50 mg kg^-1 fish. Fish (average weight 107.65 ± 34.56 g; length 21.5 ± 2.30 cm) were sampled at multiple intervals (0 to 128 h post-administration). Tissue samples (liver, kidney, gill, intestine, muscle, and plasma) were analyzed using LC-MS/MS and evaluated via a Mixed-Effects Approach with Structural Models (MAS). PZQ was absorbed most rapidly in the liver, which peaked at 205 µg kg⁻¹ in 2 h and had the highest absorption rate constant (kₐ = 1.03 h⁻¹), followed by the intestine (kₐ = 0.96 h⁻¹). Muscle tissue showed the slowest absorption (kₐ = 0.134 h⁻¹) and the longest absorption half-life (t₁/₂ₐ = 5.17 h), indicating prolonged drug retention. The highest AUC₀₋128 values were found in the gill (10,233.25 µg·h/kg) and plasma (9,309.29 µg·h/kg), reflecting greater drug exposure. Elimination varied across tissues, with muscle showing the fastest elimination (kₑ = 0.052 h⁻¹) and shortest elimination half-life (13.38 h), while the intestine exhibited the longest elimination half-life (70.18 h) and mean residence time (MRT = 102.29 h), suggesting extended retention. Model fitting showed the liver data had the highest correlation (R² = 0.978) and lowest residuals (0.944). The study characterizes the pharmacokinetic profile of praziquantel in rainbow trout, demonstrating its prolonged retention in plasma and intestinal tissues. These findings support the development of targeted dosing regimens to enhance antiparasitic efficacy, minimize tissue residues, and reduce the potential for antimicrobial resistance, thereby promoting its safe and effective use in aquaculture.</p>","PeriodicalId":23690,"journal":{"name":"Veterinary Research Communications","volume":"49 6","pages":"341"},"PeriodicalIF":2.0,"publicationDate":"2025-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145201785","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of pesticides on sperm quality in honey bee (Apis mellifera L.) drones.","authors":"Samet Okuyan, Ethem Akyol, Fırat Korkmaz, Aziz Gül, Gökhan Akdeniz, Ümit Karataş, Ahmet Emir Şahin, Sedat Sevin, Salim Aktürk, Neslihan Çakici, Serhat Solmaz","doi":"10.1007/s11259-025-10910-w","DOIUrl":"https://doi.org/10.1007/s11259-025-10910-w","url":null,"abstract":"<p><p>In this study, the effects of pesticides, which are extensively used in Turkish agriculture, on drone semen were investigated. Some of the most used pesticides in Turkey; abamectin, cypermethrin, glyphosate, and spinosad were selected. The study consisted of four pesticide groups, the solvent for these pesticides (methyl alcohol), and a control group, a total of six groups. There was a total of 36 colonies used in the experiment, with six colonies in each group. Before beginning the experiment, the colonies were homogenized, and sister queens were admitted in all colonies. When drone eggs began to form, pesticides were applied to the colonies. Sperm was taken 17 days after the drones emerged reached maturity. Semen volume and sperm count measured by spectrophotometer were recorded, and sperm concentration was calculated based on these two parameters. In addition, sperm length and viability were also assessed. When sperm concentration was examined, the lowest concentration was found in drones collected from abamectin-treated colonies (P < 0.05). The volume of ejaculates collected from bees from colonies treated with abamectin and cypermethrin was found to be lower than that of the other groups (P < 0.05). When the effects of pesticides on sperm length were examined, the sperm length of the abamectin group was the shortest, followed by the control and cypermethrin groups (P < 0.05). The sperm length of the spinosad group was the longest (P < 0.05). Sperm viability in the cypermethrin group was the lowest, followed by the abamectin group (P < 0.05). No differences were found between the control, methyl alcohol, glyphosate, and spinosad groups (P > 0.05). In the study conducted to determine the stress caused by pesticides in the larval period, it was found that abamectin and cypermethrin caused stress at the applied dose in the larval period (P < 0.05).</p>","PeriodicalId":23690,"journal":{"name":"Veterinary Research Communications","volume":"49 6","pages":"343"},"PeriodicalIF":2.0,"publicationDate":"2025-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145201759","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Parasitic dynamics of Varroa destructor and Nosema spp. and their associations with the health of honey bee (Apis mellifera) colonies in small-scale apiaries in Argentina.","authors":"Celina Maria Junges, Exequiel Furlan, Adriana A Pérez, Pablo M Beldomenico, Matías D Maggi","doi":"10.1007/s11259-025-10915-5","DOIUrl":"https://doi.org/10.1007/s11259-025-10915-5","url":null,"abstract":"<p><p>Global apiculture faces significant health challenges due to parasites such as the mite Varroa destructor and microsporidia of the genus Nosema, which can severely affect the Apis mellifera colony health and honey production. This study evaluated the dynamics of these parasites and their association with colony health in small-scale beekeeping systems. A longitudinal study was conducted in nine apiaries, with five colonies per apiary monitored over the course of an annual cycle, including two samplings during both productive and non-productive seasons. Parasite prevalence and mean abundance were assessed alongside health indicators such as brood proportions and pollen and honey reserves. Results indicated that V. destructor reached peak abundance during the productive season (p < 0.001), coinciding with colony expansion, whereas Nosema spp. peaked at the beginning of the non-productive season (p < 0.001) following acaricide treatment. These patterns suggest a competitive or opportunistic interaction wherein Varroa acts as the dominant parasite, modulating Nosema spp. infection dynamics. Additionally, eight colonies died during the study (17.7%); higher parasite loads in non-survivors suggest a potential contribution to colony losses, although causal mechanisms remain to be elucidated. During the productive season, higher open brood proportions were associated with Varroa-free colonies (p = 0.048), while mite presence correlated with increased pollen stores (p = 0.022). These findings highlight functional health indicators that are useful for monitoring parasitic dynamics in small-scale production systems and underscore the importance of integrated management strategies and long-term research to better understand parasite-host interactions in small-scale apiculture.</p>","PeriodicalId":23690,"journal":{"name":"Veterinary Research Communications","volume":"49 6","pages":"342"},"PeriodicalIF":2.0,"publicationDate":"2025-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145201844","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Unveiling Dirofilaria Asiatica infection: first clinical insights and treatment challenges for this feline zoonotic parasitosis.","authors":"Angel Almendros, Stefan Hobi, Zhou You, Tiffany Wong, Rebecca Traub","doi":"10.1007/s11259-025-10916-4","DOIUrl":"10.1007/s11259-025-10916-4","url":null,"abstract":"<p><strong>Background: </strong>Dirofilaria asiatica (previously referred to as Dirofilaria sp. Hong Kong genotype, Candidatus Dirofilaria hongkongensis, or Dirofilaria sp. \"hongkongensis\") is an emerging zoonotic filarioid nematode, initially described in human subcutaneous nodules in Hong Kong and later demonstrated in dogs and cats. This report includes the first description of clinical signs, diagnostic findings, including comparative clinicopathology, treatment and associated clinical complications in a feline infection.</p><p><strong>Methods: </strong>An 18-month-old, indoor-only spayed female Domestic Shorthair cat presented with subcutaneous nodules and microfilariae in blood smears. Diagnostic work-up included a PCR from blood, a quantitative modified Knott test, haematology, echocardiography, and abdominal ultrasonography.</p><p><strong>Results: </strong>PCR confirmed the presence of Dirofilaria asiatica. Haematology revealed neutrophilia (11.35 × 10³/µL) with left shift, and hyperproteinaemia (80 g/L). A quantitative modified Knott test revealed a microfilaremia of 36,907 per ml. Treatment with oral doxycycline and transdermal moxidectin triggered an acute onset of respiratory distress, managed with oxygen and dexamethasone. Nodules regressed, and microfilariae cleared by day 70.</p><p><strong>Conclusions: </strong>This case supports that indoor feline pets are susceptible to infection with Dirofilaria asiatica. In addition, we describe for the first time, the clinical and clinicopathological findings associated with infection and highlight the risks of anaphylactic reactions to microfilaricidal therapy. Moxidectin and doxycycline were critical for resolution, but pre-treatment with corticosteroids is recommended. Exorbitant microfilaremia might be a critical feature in cats. The zoonotic potential of this parasite warrants heightened surveillance in endemic regions.</p>","PeriodicalId":23690,"journal":{"name":"Veterinary Research Communications","volume":"49 6","pages":"338"},"PeriodicalIF":2.0,"publicationDate":"2025-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12484381/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145201815","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mycobacterial infections in cats and a dog: A case series from Southern Brazil and one health implications.","authors":"Bibiana Campello Moglia Dutra, Anelise Bonilla Trindade-Gerardi, Gabriela da Cruz Schaefer, Samara Christina França de Almeida, Bruna Correa Lopes, Cristine Cerva, Deisy Daiana Lerner, Matheus Viezzer Bianchi, Angélica Cavalheiro Bertagnolli Rodrigues, Rogério Oliveira Rodrigues, Gabriela Hartmann, David Driemeier, Daniele Mariath Bassuino, Guilherme Konradt, Franciele Maboni Siqueira, Fabiana Quoos Mayer, Flávio Silveira","doi":"10.1007/s11259-025-10906-6","DOIUrl":"https://doi.org/10.1007/s11259-025-10906-6","url":null,"abstract":"<p><p>Infections by M. avium and M. tuberculosis in companion animals are little reported. This case series describes four instances of mycobacterial infections in companion animals (three cats and one dog) in southern Brazil. We detail the diagnostic challenges and emphasize their epidemiological relevance in a One Health context. Histopathology, acid-fast bacilli staining, mycobacterial culture and PCR were used to confirm the infections. Clinical presentations, diagnostic workups, and therapeutic outcomes were reviewed. One case involved disseminated infection by M. avium in a cat with partial therapeutic success, representing the first such case reported in Rio Grande do Sul. Two cats were diagnosed with disseminated M. tuberculosis var bovis infection, all with a fatal outcome. A final case of M. tuberculosis var tuberculosis in a dog showed multisystemic dissemination and was linked to an owner previously treated for TB. These findings highlight the underrecognized role of companion animals in the epidemiology of mycobacterial infections in Brazil. Increased awareness and surveillance are needed, particularly in rural settings. A One Health approach is crucial for mitigating risks to both animal and human health.</p>","PeriodicalId":23690,"journal":{"name":"Veterinary Research Communications","volume":"49 6","pages":"336"},"PeriodicalIF":2.0,"publicationDate":"2025-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145186033","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Feasibility and safety of intrathecal transplantation of allogeneic bone marrow mesenchymal stem cells in horses.","authors":"Lucas Vinícius de Oliveira Ferreira, Leandro Maia, Danielle Jaqueta Barberini, Regina Kiomi Takahira, Vânia Maria de Vasconcelos Machado, Gisele Fabrino Machado, Guilherme Dias de Melo, Rogério Martins Amorim","doi":"10.1007/s11259-025-10920-8","DOIUrl":"https://doi.org/10.1007/s11259-025-10920-8","url":null,"abstract":"<p><p>Mesenchymal stem cells (MSCs) have emerged as a promising alternative for treating neurological disorders due to their neuroprotective, neuroregenerative, immunomodulatory, anti-inflammatory, and anti-apoptotic properties. The use of allogeneic MSCs offers advantages such as the selection of specific cells and their immediate availability. This study aimed to evaluate the safety and feasibility of intrathecal transplantation of allogeneic equine bone marrow-derived MSCs (EqBM-MSCs) in healthy horses. Ten healthy horses (five-12 years old) were randomly divided into two groups. In the control group (n = 5), phosphate-buffered saline was administered intrathecally, while the MSCs group (n = 5) received 3 × 10⁶ allogeneic EqBM-MSCs. Safety and feasibility were assessed through physical examinations monthly for one year, cerebrospinal fluid (CSF) analysis before and six days after transplantation, and magnetic resonance imaging (MRI) one-year post-transplantation. No significant differences were found between the groups in physical examinations or CSF analysis before and six days after transplantation. Furthermore, MRI showed no changes after one year. Matrix Metalloproteinase-2 (MMP-2) expression was detected in the CSF before and after transplantation but showed no significant differences. These findings suggest that intrathecal transplantation of allogeneic EqBM-MSCs is safe and viable, offering a potential therapeutic approach for neurological disorders in horses.</p>","PeriodicalId":23690,"journal":{"name":"Veterinary Research Communications","volume":"49 6","pages":"333"},"PeriodicalIF":2.0,"publicationDate":"2025-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145186910","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification of fertility associated sperm sub-populations and development of quality control assays for cryopreserved semen in buffaloes (Bubalus bubalis).","authors":"Kishan Kumar Fataniya, Arumugam Kumaresan, Anand Kumar Yadav, John Peter Ebenezer Samuel King, Muniandy Sivaram, Thirumala Rao Talluri, Ayyasamy Manimaran, Gowdar Veerappa Vedamurthy, Sakthivel Jeyakumar, Sakshi Payasi","doi":"10.1007/s11259-025-10887-6","DOIUrl":"https://doi.org/10.1007/s11259-025-10887-6","url":null,"abstract":"<p><p>The fertility of buffalo bull semen is crucial to the success of buffalo breeding programs. However, traditional methods for determining semen fertility frequently fall short, resulting in inefficiencies in artificial insemination (AI). Recent advances have highlighted the importance of sperm sub-populations as critical indicators of semen fertility. Frozen semen from 30 Mehsana buffalo bulls with known field fertility was analysed for 25 sperm traits using CASA and flow cytometry. The data was utilized to phenotypically characterize the two groups of bulls and create a model to predict their fertility. Sperm progressive motility, viability, acrosome intactness, intracellular calcium status, mitochondrial membrane potential (MMP), tyrosine phosphorylation status (PTP) and DNA fragmentation index (DFI%) differed (p < 0.05) between high and low fertility bulls. The relationship of sperm progressive motility, viability, acrosomal integrity, low intracellular calcium status, high MMP and PTP positivity were significantly positive (p < 0.05), while the relationship of acrosome reacted population, high intracellular calcium status and DFI% were significantly negative (p < 0.05) with semen fertility. Principal component analysis revealed that three components explained a total of 72.66% variation in the dataset. A stepwise regression analysis revealed that the model comprising of three variables, which include DFI%, live intracellular calcium negative and live acrosome reacted population explained 70% variation (Adjusted R² value: 0.70; p < 0.001) in the bull fertility. In conclusion, the study identified sperm quality traits explaining the variability in bull fertility, and the fertility prediction model developed in the study have the potential to be used for quality control of cryopreserved buffalo semen for obtaining high fertility in AI.</p>","PeriodicalId":23690,"journal":{"name":"Veterinary Research Communications","volume":"49 6","pages":"335"},"PeriodicalIF":2.0,"publicationDate":"2025-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145186870","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Extraskeletal chondroma of the tongue in a two-year-old quarter horse colt: a case report.","authors":"Maria Virginia Ralletti, Federica Meistro, Maria Adele Tarasconi, Luciana Mandrioli, Andrea Renzi, Riccardo Rinnovati, Alessandro Spadari","doi":"10.1007/s11259-025-10918-2","DOIUrl":"10.1007/s11259-025-10918-2","url":null,"abstract":"<p><p>Extraskeletal chondromas (ESCs) are rare benign tumours composed of mature cartilage that typically develop in soft tissues. Their occurrence in horses is extremely uncommon and, to the authors' knowledge, no previous cases of lingual ESCs in equines have been reported. This report describes a two-year-old Quarter Horse colt presented with progressive dysphagia. Clinical examination revealed a solitary, multilobulated, firm-elastic mass located at the dorsal aboral portion (base) of the tongue. Diagnostic imaging ruled out bone involvement. The mass was surgically excised under general anaesthesia, with a tracheotomy performed to secure the airways; a histopathological diagnosis of ESC was achieved. Postoperative recovery was uneventful, and at six months follow-up, the horse had fully returned to normal feeding behaviour, without signs of recurrence. This appears to be the first described case of an ESC affecting the tongue in horses, adding to the list of differential diagnoses for equine oral masses.</p>","PeriodicalId":23690,"journal":{"name":"Veterinary Research Communications","volume":"49 6","pages":"334"},"PeriodicalIF":2.0,"publicationDate":"2025-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12479599/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145186860","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Curcumin as a protective agent for goat sperm viability and DNA integrity against bisphenol A toxicity and cryopreservation stress.","authors":"Khuong Thi Thanh Tran, San Nguyen Cam Lu, Duy Lam Khanh Nguyen","doi":"10.1007/s11259-025-10878-7","DOIUrl":"https://doi.org/10.1007/s11259-025-10878-7","url":null,"abstract":"<p><p>Oxidative stress induced by environmental toxicants such as Bisphenol A (BPA) and cryopreservation significantly impairs sperm quality in goats, leading to reduced viability, motility, membrane integrity, and increased DNA fragmentation. This study aimed to evaluate the protective effects of curcumin, a natural polyphenolic antioxidant on goat sperm exposed to BPA and cryopreservation stress in vitro. Three experiments were conducted using semen collected from healthy adult goats. In Experiment 1, sperm samples were treated with 30 µM BPA to induce oxidative damage, followed by curcumin supplementation at 0, 5, 10, and 20 µM. In Experiment 2, freshly collected semen was cryopreserved in liquid nitrogen (- 196 °C) with the same curcumin concentrations added to the freezing extender. In Experiment 3, commercial frozen goat semen straws were thawed in media supplemented with curcumin at identical concentrations. Each treatment was replicated 12 times. BPA exposure significantly decreased overall motility and progressive motility compared to control. Treatment with 10 µM curcumin effectively restored sperm quality. In cryopreserved samples, 5 µM curcumin provided optimal protection. Similarly, in thawed commercial semen, 5 µM curcumin significantly improved sperm performance. These results demonstrate that curcumin at optimal concentrations effectively mitigates oxidative stress-induced damage to goat sperm, whether from BPA toxicity or cryopreservation. The dual role of curcumin during both freezing and thawing underscores its practical value as an antioxidant additive in semen preservation protocols. Its inclusion could enhance sperm quality, promote fertility outcomes, and support reproductive biotechnology applications in animal breeding programs.</p>","PeriodicalId":23690,"journal":{"name":"Veterinary Research Communications","volume":"49 6","pages":"332"},"PeriodicalIF":2.0,"publicationDate":"2025-09-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145178780","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}