Veterinary Research Communications最新文献_第2页

Distribution and infestation of Leptotrombidium scutellare (a major vector of scrub typhus) on small mammals across five provincial regions of Southwest China. 西南五省区小兽类恙虫病主要病媒scutellleptotrombidium的分布及侵染

IF 1.8 3区农林科学

Veterinary Research Communications Pub Date : 2025-07-04 DOI: 10.1007/s11259-025-10817-6

Yan Lv, Xian-Guo Guo, Peng-Wu Yin, Xiao-Bin Huang, Ti-Jun Qian

{"title":"Distribution and infestation of Leptotrombidium scutellare (a major vector of scrub typhus) on small mammals across five provincial regions of Southwest China.","authors":"Yan Lv, Xian-Guo Guo, Peng-Wu Yin, Xiao-Bin Huang, Ti-Jun Qian","doi":"10.1007/s11259-025-10817-6","DOIUrl":"https://doi.org/10.1007/s11259-025-10817-6","url":null,"abstract":"The chigger Leptotrombidium scutellare is a major vector of scrub typhus and a potential vector of hemorrhagic fever with renal syndrome (HFRS) in China. Between 2001 and 2022, a field survey was conducted at 114 survey sites in five provincial regions of southwest China, an important focus of scrub typhus and HFRS. A total of 20,254 L. scutellare were identified from 1,118 rodents and other sympatric small mammals (hosts) in 20 out of 114 survey sites, mainly concentrated in the middle longitude, latitude and altitude areas. The northwestern Yunnan (a tourist resort) was the main distribution area of L. scutellare. Most L. scutellare came from the mountainous landscape (Cr=82.73%) and outdoor habitat (Cr=99.21%). The hosts of L. scutellare crossed four orders, eight families, 19 genera and 33 species with low host specificity. More than half of L. scutellare mites (56.55%) came from the vole Eothenomys miletus with high infestation (PM=17.99%, MA = 4.24, MI = 23.57). Leptotrombidium scutellare was of aggregated distribution among different individuals of its hosts, and it had sex and age bias of host selection with preference to male and adult hosts. The landscape, altitude, longitude, latitude and host were important factors influencing the distribution and infestation of L. scutellare. The occurrence of abundant L. scutellare with low host specificity in southwest China (especially in the tourist resort of northwestern Yunnan) would increase the risk of transmission and focus-persistence of scrub typhus and HFRS in the region.","PeriodicalId":23690,"journal":{"name":"Veterinary Research Communications","volume":"49 4","pages":"246"},"PeriodicalIF":1.8,"publicationDate":"2025-07-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144561324","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Fatal coinfection with Hepatozoon canis, Babesia vogeli, Ehrlichia canis, and Bartonella clarridgeiae in a dog from Argentina. 阿根廷一只犬同时感染犬肝虫、沃氏巴贝斯虫、犬埃利希体和巴尔通体致死性感染。

IF 1.8 3区农林科学

Veterinary Research Communications Pub Date : 2025-07-03 DOI: 10.1007/s11259-025-10810-z

María Celeste Facelli Fernández, Johann Barolin, Antonela Florencia Stassi, Lucas Daniel Monje, Eduardo Matías Belotti

{"title":"Fatal coinfection with Hepatozoon canis, Babesia vogeli, Ehrlichia canis, and Bartonella clarridgeiae in a dog from Argentina.","authors":"María Celeste Facelli Fernández, Johann Barolin, Antonela Florencia Stassi, Lucas Daniel Monje, Eduardo Matías Belotti","doi":"10.1007/s11259-025-10810-z","DOIUrl":"https://doi.org/10.1007/s11259-025-10810-z","url":null,"abstract":"Vector-borne diseases represent a growing global threat, exposing new populations to previously unknown infectious agents, and posing diagnostic challenges for veterinarians. The aim of this article is to present a clinical case of coinfection with different hemoparasites in a canine with severe ectoparasite infestation and clinical signs of shock. Blood smears and complete necropsy were performed. Samples of different organs were obtained for histopathological and molecular biology studies. Examination of the blood smears showed azurophilic intracytoplasmic structures, compatible with the morphology of Ehrlichia canis, monocytes with the presence of gametocytes inside neutrophils, compatible with infection with Hepatozoon canis, and pyriform intraerythrocyte structures compatible with large piroplasms. Acridine orange staining, performed on different histological sections, allowed visualizing textures on fluorescent microscopy analysis, which were morphologically compatible with Bartonella sp. Molecular tests were positive for Ehrlichia spp., Hepatozoon spp., Babesia spp. and Bartonella spp. Control of vector-borne diseases would improve the health and well-being of animals and humans, especially in developing countries in the tropics, where the risk of contracting these diseases is high and access to health care is poor. Vector-borne diseases should always be suspected and included in the differential diagnosis, especially in areas with the presence of ticks and fleas.","PeriodicalId":23690,"journal":{"name":"Veterinary Research Communications","volume":"49 4","pages":"244"},"PeriodicalIF":1.8,"publicationDate":"2025-07-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144555015","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Disseminated aspergillosis associated with severe non-regenerative anemia in a Lagotto Romagnolo: a case report. 罗马Lagotto地区播散性曲霉病伴严重非再生性贫血1例。

IF 1.8 3区农林科学

Veterinary Research Communications Pub Date : 2025-07-02 DOI: 10.1007/s11259-025-10809-6

Maria Teresa Antognoni, Valentina Cremonini, Francesco Birettoni, Fabrizio Passamonti, Ambra Lisa Misia, Aurora Barbetta, Arianna Miglio

引用次数: 0

High-risk Escherichia coli global clones ST10 and ST155 in wild raptors admitted to a rehabilitation center. 野生猛禽体内的高风险大肠杆菌全球克隆体ST10和ST155被送入康复中心。

IF 1.8 3区农林科学

Veterinary Research Communications Pub Date : 2025-07-01 DOI: 10.1007/s11259-025-10811-y

Bruna B R Garcia, Guilherme E Paiva, Matheus U B Silva, João Pedro Rueda Furlan, Bruna Fuga, Paula R Lemos, Jose H Fontenelle, Nilton Lincopan, Fábio P Sellera

{"title":"High-risk Escherichia coli global clones ST10 and ST155 in wild raptors admitted to a rehabilitation center.","authors":"Bruna B R Garcia, Guilherme E Paiva, Matheus U B Silva, João Pedro Rueda Furlan, Bruna Fuga, Paula R Lemos, Jose H Fontenelle, Nilton Lincopan, Fábio P Sellera","doi":"10.1007/s11259-025-10811-y","DOIUrl":"https://doi.org/10.1007/s11259-025-10811-y","url":null,"abstract":"The dissemination of extended-spectrum β-lactamase (ESBL)-producing Enterbacterales, once largely confined to healthcare settings, has become a growing One Health concern as these pathogens threaten diverse environments, including wildlife habitats. This study describes high-risk clones of ESBL-producing Enterobacterales in wildlife undergoing rehabilitation at a zoological park in Southeast Brazil. Among 49 wild mammals and birds sampled, two Escherichia coli strains, from a rusty-barred owl and a black vulture, tested positive for ESBL production and exhibited multidrug resistance profiles. Genomic characterization identified these strains as belonging to globally recognized clones of the sequence type (ST) ST10 and ST155, which are associated with widespread antimicrobial resistance (AMR) and severe infections in human and veterinary medicine. The strains harbored resistance genes for several antimicrobials and virulence factors typically found in extraintestinal pathogenic E. coli. ESBL-encoding genes were related to mobile genetic elements, with the blaCTX-M-15 gene embedded in a multidrug resistance region flanked by Tn3 and IS26 elements and the blaCTX-M-2 gene located on a complex class 1 integron. Accordingly, this study spotlights the potential role of wildlife rehabilitation centers as reservoirs for critical AMR, emphasizing the importance of monitoring such facilities for the presence of priority pathogens. Therefore, this study reinforces the necessity for microbiological surveillance in wildlife rehabilitation settings to prevent the spread of AMR into natural ecosystems and calls for the development of standardized guidelines for the reintroduction of animals to ensure that colonized wildlife do not contribute to the dissemination of resistant pathogens in the wild.","PeriodicalId":23690,"journal":{"name":"Veterinary Research Communications","volume":"49 4","pages":"241"},"PeriodicalIF":1.8,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144545083","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Development of TaqMan real-time PCR and droplet digital PCR protocols for the detection of Candidatus Midichloria mitochondrii and evaluation of exposure among wildlife. TaqMan实时PCR和液滴数字PCR检测Midichloria线粒体及野生动物暴露评价方法的建立。

IF 1.8 3区农林科学

Veterinary Research Communications Pub Date : 2025-07-01 DOI: 10.1007/s11259-025-10808-7

Alessia Pucciarelli, Lorena Cardillo, Maurizio Viscardi, Gerardo Picazio, Nicola D'Alessio, Giovanni Sgroi, Antonio Rinaldi, Vincenzo Veneziano, Giovanna Fusco, Claudio de Martinis

{"title":"Development of TaqMan real-time PCR and droplet digital PCR protocols for the detection of Candidatus Midichloria mitochondrii and evaluation of exposure among wildlife.","authors":"Alessia Pucciarelli, Lorena Cardillo, Maurizio Viscardi, Gerardo Picazio, Nicola D'Alessio, Giovanni Sgroi, Antonio Rinaldi, Vincenzo Veneziano, Giovanna Fusco, Claudio de Martinis","doi":"10.1007/s11259-025-10808-7","DOIUrl":"10.1007/s11259-025-10808-7","url":null,"abstract":"Candidatus Midichloria mitochondrii (Ca. M. mitochondrii), an endosymbiont intracellular bacterium living in the mitochondria of several tick species, especially ixodid species, has been proposed as a potential marker for monitoring tick-bite exposure. Therefore, the present study aimed to develop two different diagnostic methods, TaqMan-based real-time PCR (rt-PCR) and Droplet Digital PCR (dd-PCR), targeting the 16 S rRNA gene and gyrB gene for the detection of Ca. M. mitochondrii in different wildlife species from several areas of southern Italy. Both techniques were validated using 10-fold serial dilutions of a sequenced positive control up to reach 10-6 final dilution. Among wildlife field samples, both the techniques identified Ca. M. mitochondrii DNA, although dd-PCR showed higher sensitivity, being able to detect the target DNA in a higher dilution and in several spleen samples scored negative by rt-PCR. Noteworthy, these molecular methods revealed for the first time the presence of Ca. M. mitochondrii DNA in red foxes (Vulpes vulpes), Eurasian badgers (Meles meles), otters (Lutra lutra), porcupines (Hystrix cristata), European hares (Lepus europaeus), and alpacas (Vicugna pacos), suggesting a tick-bite exposure of these animals in the study area.","PeriodicalId":23690,"journal":{"name":"Veterinary Research Communications","volume":"49 4","pages":"240"},"PeriodicalIF":1.8,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12214003/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144545081","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Novel paramyxovirus in wild pinnipeds, Brazil. 巴西野生鳍足类动物的新型副粘病毒。

IF 1.8 3区农林科学

Veterinary Research Communications Pub Date : 2025-07-01 DOI: 10.1007/s11259-025-10799-5

Samira Costa-Silva, Ana Carolina Ewbank, Aricia Duarte-Benvenuto, Carlos Sacristán, Rodrigo M Soares, Angélica M Sánchez-Sarmiento, Natália Silvestre-Perez, Pedro Volkmer Castilho, Cristiane K M Kolesnikovas, Caroline Freitas Pessi, Rafael Sardinha Murro, Carla B Barbosa, Raquel B Ferioli, José L Catão-Dias, Lara B Keid

{"title":"Novel paramyxovirus in wild pinnipeds, Brazil.","authors":"Samira Costa-Silva, Ana Carolina Ewbank, Aricia Duarte-Benvenuto, Carlos Sacristán, Rodrigo M Soares, Angélica M Sánchez-Sarmiento, Natália Silvestre-Perez, Pedro Volkmer Castilho, Cristiane K M Kolesnikovas, Caroline Freitas Pessi, Rafael Sardinha Murro, Carla B Barbosa, Raquel B Ferioli, José L Catão-Dias, Lara B Keid","doi":"10.1007/s11259-025-10799-5","DOIUrl":"10.1007/s11259-025-10799-5","url":null,"abstract":"Most emerging zoonotic viral infections originate in wildlife. The Paramyxoviridae and Coronaviridae families are important in terms of zoonotic potential and diversity of host susceptibility. To date, the only paramyxoviruses described in pinnipeds are within the genus Morbillivirus. An alphacoronavirus has been reported in pinnipeds. Herein, we surveyed for Paramyxoviridae and Coronaviridae in 46 animals of four pinniped species stranded along the coasts of Santa Catarina and São Paulo states, Brazil, between 2016 and 2022. A novel paramyxovirus (family Paramyxoviridae) was detected in an Antarctic fur seal (Arctocephalus gazella) and in two South American fur seals (Arctocephalus australis). The sequences presented with less than 83% amino acid identity to the closest published paramyxovirus sequences, amplified in Microchiroptera sp. in Vietnam and in Asiatic yellow bats (Scotophilus kuhlii) in Cambodia. Phylogenetic analysis of the paramyxovirus sequences clustered into the genus Jeilongvirus. Gross and histopathological analyses did not identify any related lesions and the viral zoonotic potential is unknown. None of the animals were PCR-positive to coronavirus. To our knowledge, this is the first report of paramyxovirus infection in pinnipeds in Brazil and the first paramyxovirus not classified as Morbillivirus in seals, sea lions and walruses. To better understand the natural history of this novel virus, future pathological, serological, and molecular studies based on pan-Paramyxoviridae RT-PCRs are recommended.","PeriodicalId":23690,"journal":{"name":"Veterinary Research Communications","volume":"49 4","pages":"242"},"PeriodicalIF":1.8,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12213916/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144545084","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Differences between behavioral time budget and welfare indicators in two different slow-growing broiler genotypes kept in the free-range system. 散养两种不同基因型慢生长肉鸡行为时间预算和福利指标的差异

IF 1.8 3区农林科学

Veterinary Research Communications Pub Date : 2025-06-28 DOI: 10.1007/s11259-025-10814-9

Arda Sözcü, Aydın İpek, Stefan Gunnarsson

{"title":"Differences between behavioral time budget and welfare indicators in two different slow-growing broiler genotypes kept in the free-range system.","authors":"Arda Sözcü, Aydın İpek, Stefan Gunnarsson","doi":"10.1007/s11259-025-10814-9","DOIUrl":"10.1007/s11259-025-10814-9","url":null,"abstract":"The consumer interest for meat from slow growing broilers in free-range system has increased recently. Therefore, the need for knowledge about behaviour and welfare of birds in these systems has increased. The aim of this study was to compare the differences between behavioral time budget, tonic immobility and clinical welfare indicators in two slow growing broiler genotypes (Hubbard ISA Red JA-57 and Sasso XL44 × SA51A) kept in a free-range system. In total, 480 one-day old chicks were reared, and the birds were regularly scored for behavioral time budget and multiple welfare indicators. The eating and drinking tended to decrease in Sasso birds, whereas they showed an increment in Hubbard birds with increasing of age (P < 0.01). Hubbard birds had the highest percentage of explorative pecking (7.65%) of the total time budget compared to the Sasso birds (4.33% at day 63, P < 0.01). Comb pecking wounds, skin injuries and gait scores were affected by both genotype and age (P < 0.01). The duration of tonic immobility was found to be longer, as well as the number of tonic immobility inductions was higher in Sasso birds compared to the Hubbard (26.49 vs. 19.68 s; 1.54 and 1.24, respectively P < 0.01). These findings indicate that birds of the Hubbard genotype may be more prone to comb pecking and skin injuries, but they showed less fearful and higher walking ability, compared to Sasso birds.","PeriodicalId":23690,"journal":{"name":"Veterinary Research Communications","volume":"49 4","pages":"239"},"PeriodicalIF":1.8,"publicationDate":"2025-06-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12206162/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144529825","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Surface temperature changes indicate disease onset after pulmonary murine corona virus infection, but do not constitute a humane endpoint. 表面温度变化表明肺部小鼠冠状病毒感染后疾病发作，但不构成人道终点。

IF 1.8 3区农林科学

Veterinary Research Communications Pub Date : 2025-06-28 DOI: 10.1007/s11259-025-10806-9

Rebecca Nistelberger, Patrizia Gibler, Thomas Filip, Manuel Salzmann, Boris Hartmann, Bruno K Podesser, Roberto Plasenzotti, Philipp J Hohensinner, Julia B Kral-Pointner

{"title":"Surface temperature changes indicate disease onset after pulmonary murine corona virus infection, but do not constitute a humane endpoint.","authors":"Rebecca Nistelberger, Patrizia Gibler, Thomas Filip, Manuel Salzmann, Boris Hartmann, Bruno K Podesser, Roberto Plasenzotti, Philipp J Hohensinner, Julia B Kral-Pointner","doi":"10.1007/s11259-025-10806-9","DOIUrl":"10.1007/s11259-025-10806-9","url":null,"abstract":"Mouse models are important contributors for understanding the immune system during infections. Objective parameters help to assess the course of infection and guarantee animal welfare. In this study we analyzed if surface temperature measured via thermal imaging of the dorsal area is a suitable marker to evaluate animal wellbeing during murine coronavirus (MCoV) infection. Infected BALB/c mice displayed severe symptoms whereas C57BL/6 mice were less affected. In BALB/c animals, temperature increased from 27.1 °C to 28.4 °C within 24 h with levels remaining slightly elevated over the observation period. In contrast, a decrease in body weight was consistent through the period with 60% of the animals reaching the previously set termination point of 20% weight loss (n = 6). Also, C57BL/6J animals showed a significant temperature increase from 27.1 °C to 28.4 °C within 24 h and a significant weight loss over time with two out of ten reached weight loss end point. However, temperature and weight changes were not related in individual animals. In contrast to temperature values, body weight clearly set a trajectory towards early termination. Taken together, our data indicate that superficial temperature did not serve as a predictive parameter for defining humane end points, but indicate disease onset after pulmonary virus infection.","PeriodicalId":23690,"journal":{"name":"Veterinary Research Communications","volume":"49 4","pages":"238"},"PeriodicalIF":1.8,"publicationDate":"2025-06-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12206167/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144529826","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

First report on whole saliva profiling of Landlly pigs for stress, inflammation, immunity, and systemic homeostasis appraisal: a pilot study. 首次报道了兰利猪的全唾液分析，用于应激、炎症、免疫和系统稳态评估：一项初步研究。

IF 1.8 3区农林科学

Veterinary Research Communications Pub Date : 2025-06-28 DOI: 10.1007/s11259-025-10805-w

Mayur M Jadav, Vivek Joshi

{"title":"First report on whole saliva profiling of Landlly pigs for stress, inflammation, immunity, and systemic homeostasis appraisal: a pilot study.","authors":"Mayur M Jadav, Vivek Joshi","doi":"10.1007/s11259-025-10805-w","DOIUrl":"https://doi.org/10.1007/s11259-025-10805-w","url":null,"abstract":"Given the stressful nature of blood collection, it is ideal to use saliva as a source of biomarkers that can reveal information about the health of pigs. Nevertheless, no research on the characterization of salivary analytes has been undertaken for Landlly pigs. In this study, the viability of two saliva sampling procedures was tested and a sialochemistry panel was developed. A total of 30 Landlly (Landrace x Ghurrah) pigs (15 males, 15 females) in their growing phase (8-10 weeks old) were chosen to obtain the normal reference ranges of 13 salivary analytes, specifically those related to stress (cortisol, α-amylase), inflammation (diamine oxidase, DAO; calprotectin, CALP), immune status (IgA, Cu, Zn) and systemic homeostasis (glucose, total protein, Na, K, Mg, Fe). The 'cotton rope method' allowed the pigs to gnaw on a hanging cotton rope for half an hour while the 'cotton swab method' included swabbing a piece of cotton (5 cm × 5 cm) inside the pigs' mouths for five minutes. Saliva was then removed by squeezing the wet materials and centrifugation. The cotton swab method achieved the best combination of reduced sampling time and low contamination. The overall mean salivary values of cortisol, α-amylase, DAO, CALP, IgA, Cu and Zn were 40.01 ng/ml, 2086.60 IU/L, 26.76 ng/ml, 0.63 ng/ml, 116.82 ng/ml, 123.33 µg/dl and 49.43 µg/dl, respectively. Moreover, the mean salivary values were 1.71 mg/dl, 0.89 g/dl, 95.74 mmol/l, 8.94 mmol/l, 0.41 mmol/l, and 5.33 μg/dl for glucose, TP, Na, K, Mg, and Fe, respectively. All salivary analytes exhibited a positive correlation with their serum equivalents, except for K and Fe. These preliminary findings illustrate that the cotton rope method works well for pooled saliva sampling while for individual saliva samples, the cotton swab approach is appropriate. Salivary analytics has a high potential for usage in farm settings as a quick, easy, affordable, and non-invasive diagnostic method for Landlly pigs.","PeriodicalId":23690,"journal":{"name":"Veterinary Research Communications","volume":"49 4","pages":"237"},"PeriodicalIF":1.8,"publicationDate":"2025-06-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144512569","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The effect of melatonin implants on the embryo yield and oxidative stress levels in superovulated Holstein heifers. 褪黑素植入对超排卵荷斯坦母牛胚胎产量和氧化应激水平的影响。

IF 1.8 3区农林科学

Veterinary Research Communications Pub Date : 2025-06-24 DOI: 10.1007/s11259-025-10807-8

Majid Gholami, Najmeh Davoodian, Ali Kadivar, Naser Shams-Esfandabadi, Hassan Nazari

{"title":"The effect of melatonin implants on the embryo yield and oxidative stress levels in superovulated Holstein heifers.","authors":"Majid Gholami, Najmeh Davoodian, Ali Kadivar, Naser Shams-Esfandabadi, Hassan Nazari","doi":"10.1007/s11259-025-10807-8","DOIUrl":"10.1007/s11259-025-10807-8","url":null,"abstract":"Superstimulation of ovaries predisposes the donors to oxidative stress, potentially impacting the outcomes. This study evaluated the effect of melatonin implants on embryo yield and oxidative stress levels in Holstein heifers, who were allocated to two groups: one (14 heifers) received melatonin implants (18 mg/50kg) before the start of superovulation, while the other (10 heifers) did not. Serum samples were collected on days 0, 9, and 16 to measure total antioxidant capacity (TAC) and total oxidant status (TOS). Ovarian follicles, corpora lutea, and recovered embryos were recorded at D16. Superovulation and Fertilization responses were not different; Ovulation response increased (80.73 ± 4.94 vs. 57.02 ± 10.61) in the treatment compared to the control. The mean number of CLs and Total recovered embryos were not different; Transferable embryos (3.85 ± 0.91 vs. 1.8 ± 0.37) were higher in treatment compared to the control (p˂0.05). Blastocysts and degenerated embryos were not different. TOS in the treatment group decreased at D7 and D16 compared with D0 (p˂0.05) but increased over time in control (p˂0.05); it was not different between groups at D0 but was lower in treatment than control at D9 and D16 (p˂0.05). TAC didn't change over time; however, it was higher in treated than in control donors at D7 (p˂0.05). OSI was lower in the treatment than control group at D9 and D16. Conclusively, melatonin implants in superovulated heifer donors increased the number of transferable embryos and reduced anovulated follicles, thereby enhancing ovulation and embryo quality. It mitigated oxidative stress by decreasing total oxidant status and boosting total antioxidant capacity.","PeriodicalId":23690,"journal":{"name":"Veterinary Research Communications","volume":"49 4","pages":"236"},"PeriodicalIF":1.8,"publicationDate":"2025-06-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144476912","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0