Veterinary Research Communications最新文献

{"title":"Age- and agro-climatic zone-specific variations in post-vaccinal antibody responses to FMD vaccination in bovine populations: a longitudinal study from Karnataka, India.","authors":"Jagadish B Hiremath, Rohini Bhat, G B Bhavana, Sagar Awati, Shanthkumar B Mannapur, Manjunatha Reddy Gundallahalli, Sharanagouda S Patil, Satheesha S P, Divakar Hemadri, Suresh K P, Saravanan Subramaniam, Raveendra Hegde, Rahman H","doi":"10.1007/s11259-025-10737-5","DOIUrl":"https://doi.org/10.1007/s11259-025-10737-5","url":null,"abstract":"<p><p>This longitudinal study examined variations in post-vaccination antibody response to Foot-and-Mouth Disease (FMD) vaccination in bovines across different age groups and agro-climatic zones in Karnataka, India. A total of 374 serum samples were collected from 28 villages across nine agroclimatic zones, targeting pre- and post-vaccination titers for FMD virus serotypes O, A, and Asia-1. Analysis via single-dilution liquid-phase blocking ELISA revealed significant findings: pre-vaccination immune proportions were below the herd immunity threshold (< 80%) for serotypes O and Asia-1 but exceeded it for serotype A. Younger bovines (< 24 months) displayed notably lower antibody titers (log₁₀1.67-1.99) compared to older cattle (> 24 months; log₁₀2.14-2.44), highlighting the age-dependent vaccine response variability. Agroclimatic disparities were evident, with regions like the Coastal and Central Dry Zones failing to achieve protective titers (log₁₀<1.8) even post-vaccination, whereas zones like the Eastern Dry Zone demonstrated marked improvements. Post-vaccination seromonitoring showed an overall increase in immune proportions for all serotypes, yet younger cattle and certain agroclimatic zones remained suboptimal. These findings emphasize the importance of region-specific vaccination strategies and the need for tailored booster protocols to enhance immunity in younger bovines. The study underscores the utility of seromonitoring in optimizing FMD control programs by addressing age and agro-climatic disparities in immune responses.</p>","PeriodicalId":23690,"journal":{"name":"Veterinary Research Communications","volume":"49 3","pages":"167"},"PeriodicalIF":1.8,"publicationDate":"2025-04-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144048881","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of chemical elements as potential biomarkers in the treatment of goat mastitis.","authors":"Ugonna Henry Uzoka, Juliana Miwa Fujikura, Richard Costa Polveiro, Jéssica Lobo Albuquerque Caldeira, Maria Júlia Fernandes Netto, Luísa Maria de Souza Menezes, Cristiane do Carmo Cesário, Fabricio Luciani Valente, Maria Aparecida Scatamburlo Moreira","doi":"10.1007/s11259-025-10733-9","DOIUrl":"https://doi.org/10.1007/s11259-025-10733-9","url":null,"abstract":"<p><p>Mastitis significantly impacts dairy goat herds, causing substantial economic losses. The escalating challenge of multidrug-resistant bacteria has prompted research into alternative therapeutic strategies, including milk microbiota transfer and phytochemical treatments. This study evaluates milk chemical elements as potential biomarkers during alternative treatments for Staphylococcus warneri-induced goat mastitis. Seven female Parda Alpina goats were studied, with six receiving sequential treatments: milk microbiota transplantation (MMT) from the seventh goat (donor), two days later, intra-mammary 7-epiclusianone administration followed. The right udder of the six goats received the treatments and different time points while the left udder served as the control. Milk samples (n = 120) were analyzed using X-ray Energy Dispersive Spectroscopy (XEDS) to monitor trace and macro elements. Milk elemental level variations were observed across both treatments. Notable changes included increased iron levels from MMT initiation, reduced copper and zinc levels during MMT, and decreased sodium levels following 7-epiclusianone treatment. These results suggest zinc, copper, iron, and sodium could serve as potential biomarkers for monitoring mastitis treatment effectiveness. Additional research with broader and more diverse sample populations would help validate these findings and explore the method's broader applicability.</p>","PeriodicalId":23690,"journal":{"name":"Veterinary Research Communications","volume":"49 3","pages":"166"},"PeriodicalIF":1.8,"publicationDate":"2025-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144039079","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Rabies virus, Bartonella koehlerae and Leishmania infantum coinfection in a black Myotis (Myotis nigricans) from southeastern Brazil.","authors":"Danilo Alves de França, Bruna Letícia Devidé Ribeiro, Benedito Donizete Menozzi, Helio Langoni","doi":"10.1007/s11259-025-10736-6","DOIUrl":"https://doi.org/10.1007/s11259-025-10736-6","url":null,"abstract":"<p><p>The aim of this study was to investigate the presence of important zoonotic agents in Brazil in urban bats and to evaluate the existence of coinfections in bats diagnosed with rabies. In 2021, organ samples from 52 bats from urban areas in southeastern Brazil were used to diagnose rabies and other zoonoses occurring in the region. The positive samples were sequenced, characterized and included in GenBank. A co-infection involving the rabies virus, Bartonella koehlerae, and Leishmania infantum was identified in a Black Myotis bat collected from a household in São Manuel, a city endemic for visceral leishmaniasis. Phylogenetic analysis showed similarity between the RABV sequence obtained and reference sequences from humans and wild canids, as well as confirming the identity of the Bartonella and Leishmania species detected. To the best of our knowledge, this is the first report of a bat co-infected with rabies virus, B. koehlerae, and L. infantum. The co-infection of three important pathogens in a Black Myotis highlights the multifaceted role of neotropical bats as reservoirs of zoonotic agents. This unprecedented finding reinforces the potential of these animals to contribute to the transmission dynamics of viral, bacterial and protozoan pathogens, and the importance of molecular surveillance in bats.</p>","PeriodicalId":23690,"journal":{"name":"Veterinary Research Communications","volume":"49 3","pages":"165"},"PeriodicalIF":1.8,"publicationDate":"2025-04-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144055297","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Prenatal ultrasound diagnosis of ectopic ureter and renal hypoplasia in two puppies: a case report.","authors":"H Moserová, L Frgelecová, R Morávek, P Proks","doi":"10.1007/s11259-025-10732-w","DOIUrl":"https://doi.org/10.1007/s11259-025-10732-w","url":null,"abstract":"<p><p>This study reports two cases of prenatal ultrasound diagnosis of ectopic ureter and bilateral renal hypoplasia in two different canine fetuses. These developmental disorders were detected during a routine pregnancy ultrasound examination of a female Golden retriever and a female Old English bulldog. A suspected diagnosis of ectopic ureter was made based on the ultrasonographic detection of fetal kidney hydronephrosis and ureteral dilatation, along with consideration of breed predisposition. Bilateral fetal renal hypoplasia presented with ultrasonographic detection of reduced, hyperechogenic fetal kidneys without a distinguishable renal pelvis. Ultrasonographic findings were subsequently confirmed postnatally at surgery or autopsy and histopathology. Our findings highlight the potential for early detection of urogenital anomalies in canines and the importance of knowledge of normal fetal anatomy during ultrasound examination of pregnancy.</p>","PeriodicalId":23690,"journal":{"name":"Veterinary Research Communications","volume":"49 3","pages":"163"},"PeriodicalIF":1.8,"publicationDate":"2025-04-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11991952/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144041123","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bovine brucellosis sero-prevalence trend during 2011-14 and 2015-18 in Indian sub-continent.","authors":"Rajeswari Shome, Sharangauda Patil, Somy Skariah, Snigdha Madhaba Maharana, G Shanmugam, Kuralayanapalya Puttahonnappa Suresh, Thimmappa Rajeshwari Nanditha, Nagalingam Mohandoss","doi":"10.1007/s11259-025-10731-x","DOIUrl":"https://doi.org/10.1007/s11259-025-10731-x","url":null,"abstract":"<p><p>Brucellosis, a significant zoonotic disease impacting livestock and human health poses substantial economic and public health challenges in endemic regions. This study analyses and compares the sero-prevalence of bovine brucellosis across 14 states in India over two distinct periods: 2011-14 and 2015-18. A stratified random sampling approach and a protein G-based indirect Enzyme Linked Immunosorbent Assay (iELISA) were employed to evaluate 6408 bovine serum samples during 2015-18 (cattle- 4495; buffaloes- 1913) and results were compared to published data of 2011-14. Chi-square test and analysis of variance (ANOVA) were carried out to know the significance among the states and also between the two time periods. Bovine brucellosis sero-prevalence across Indian states showed a significant decline from 2011 to 14 to 2015-18. In cattle, sero-prevalence reduced from 9.60 to 1.76% (p < 0.0001) with notable reductions in seven states (Gujarat, Madhya Pradesh, Karnataka, Kerala, Maharashtra, Tamil Nadu, and Rajasthan). Except in the state of Punjab, highest sero-prevalence was noted despite a significant decrease (p = 0.002). Whereas, in buffaloes, prevalence declined from 5.35 to 2.67% (p < 0.0001), with significant reduction in two out of ten states tested with modest decreases in other states. The study revealed that only 2 out of 10 states showed reduced brucellosis sero-prevalence in buffaloes indicating the need for aggressive control efforts and continued investment in surveillance, vaccination, and public health education.</p>","PeriodicalId":23690,"journal":{"name":"Veterinary Research Communications","volume":"49 3","pages":"164"},"PeriodicalIF":1.8,"publicationDate":"2025-04-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144030211","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of dietary egg lysozyme on improved growth performance, intestinal health, antioxidant capacity, and disease resistance in hybrid red tilapia (Oreochromis niloticus x O. mossambicus).","authors":"Mahmoud Radwan, Saad M Alsaiad, Ghadeer M Albadrani, Muath Q Al-Ghadi, Mohamed M Abdel-Daim, Mohamed M M El-Feky","doi":"10.1007/s11259-025-10719-7","DOIUrl":"https://doi.org/10.1007/s11259-025-10719-7","url":null,"abstract":"<p><p>This study evaluated the dietary lysozyme (DSLY) effect on the growth performance, health status, and disease resistance in hybrid red tilapia (Oreochromis niloticus x O. mossambicus). Fish were fed dietary DSLY at 0, 1, 2, and 3 g/kg diet (L0-L3, respectively) for 60 days, followed by a challenge with Streptococcus agalactiae. Fish-fed diets (DSLY) showed significant improvements in growth, carcass composition, digestive enzyme activity, and intestinal histomorphometry compared to control groups, specifically in the L2 and L3 groups. Fish fed with DSLY had lower gut bacterial counts than the control. Fish-fed DSLY positively affected hemato-biochemical indices compared to the control group, especially in the 2 and 3 g/kg diets. Comparing fish fed DSLY to those fed 0 g/kg lysozyme, particularly in the 2 and 3 g/kg diets, revealed a significant decrease in MDA levels along with an increase in antioxidant (SOD and CAT) and immunological indices. Fish-fed DSLY, after the challenge with S. agalactiae showed a lower mortality rate than the control. The histological structure of the intestines and stomach of fish that fed DSLY improved. These results suggest that fish-fed DSLY enhanced their intestinal health and growth, boosted their immune responses, and raised their resistance to S. agalactiae.</p>","PeriodicalId":23690,"journal":{"name":"Veterinary Research Communications","volume":"49 3","pages":"162"},"PeriodicalIF":1.8,"publicationDate":"2025-04-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144030198","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Fumonisin B1 induces oxidative stress, inflammation and necroptosis in IPEC-J2 cells.","authors":"Yinuo Zou, Xinyu Du, Xiaoyan Zheng, Sichao Mao, Xinyi Xu, Shuxia Zhang, Xingxiang Chen","doi":"10.1007/s11259-025-10728-6","DOIUrl":"10.1007/s11259-025-10728-6","url":null,"abstract":"<p><p>Fumonisin B1 (FB1), an important mycotoxin, poses a significant threat to public health and livestock production due to its widespread contamination. Furthermore, the gastrointestinal tract is particularly vulnerable to FB1 exposure given its frequent contamination of staple crops such as corn. Although necroptosis has been recognized as a critical mechanism underlying intestinal damage caused by certain environmental toxins, whether FB1 specifically triggers necroptosis in intestinal epithelial cells remains to be fully elucidated. In this study, the intestinal porcine epithelial cell line-J2 (IPEC-J2) was employed as an in vitro model to study the intestinal cells injury caused by FB1 and the underlying mechanisms. By measuring IPEC-J2 cell viability, intracellular reactive oxygen species, gene levels, and protein levels, it was found that FB1 dose-dependent induced IPFC-J2 cell injury, oxidative stress, and inflammation. Meanwhile, FB1 significantly increased the expression of necroptosis-related genes and proteins in IPEC-J2 cells, indicating that FB1 induced the occurrence of necroptosis. In summary, the results demonstrated FB1 can induce oxidative stress, inflammation and necroptosis in IPEC-J2 cells.</p>","PeriodicalId":23690,"journal":{"name":"Veterinary Research Communications","volume":"49 3","pages":"161"},"PeriodicalIF":1.8,"publicationDate":"2025-04-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143812513","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bacillus velezensis MFF 2.2-derived lipopeptides modulate gut immune response in BALB/c mice maintaining mucosal integrity and systemic safety.","authors":"Ana Lucía Di Giácomo, Lorena Nadia Azcurra, Gisela Romina García, José María Raviolo, María Carolina Grosso, Cecilia Ana Dogi, María Laura González Pereyra","doi":"10.1007/s11259-025-10730-y","DOIUrl":"10.1007/s11259-025-10730-y","url":null,"abstract":"<p><p>Bacillus-derived lipopeptides, particularly surfactin (SF), are known for their antimicrobial and immunomodulatory properties, making them promising candidates for applications in veterinary medicine. This study investigated the immunomodulatory, cytotoxic, and genotoxic effects of a lipopeptide extract (LPE) derived from Bacillus velezensis MFF 2.2, containing SF, using an in vivo murine model. Four groups of BALB/c mice (n = 6 per group) were orally administered daily doses of: (1) saline solution (control), (2) 60 μg/mL SF standard, (3) 40 μg/mL LPE, and (4) 60 μg/mL LPE, over a 10-day period. Assessments included changes in body weight, histopathological analysis of liver, kidney, and small intestine tissues, as well as evaluation of cytotoxic and genotoxic effects. LPE treatment was well-tolerated, with no significant changes in body weight, organ histopathology, or bone marrow toxicity. Both SF and LPE significantly increased goblet cell counts (P < 0.05), while LPE reduced IFN-γ levels and increased the IL- 10/IFN-γ ratio, suggesting an anti-inflammatory response (P < 0.05). TNF-α and IL- 10 levels remained unchanged, and no significant differences were observed in intraepithelial lymphocyte counts. These findings suggest that LPE derived from B. velezensis MFF 2.2 is safe and promotes immunomodulatory effects, supporting its potential as a therapeutic agent in inflammatory conditions.</p>","PeriodicalId":23690,"journal":{"name":"Veterinary Research Communications","volume":"49 3","pages":"159"},"PeriodicalIF":1.8,"publicationDate":"2025-04-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143796463","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Seroepidemiological study of Leishmania infantum, Toxoplasma gondii and Dirofilaria immitis in pet ferrets (Mustela putorius furo) in Spain.","authors":"José Villora, María Eugenia Lebrero, Jacobo Giner, Asier Basurco, Laura Vilalta, Andrés Montesinos, Maria-Magdalena Alcover, Cristina Riera, Roser Fisa, Xavier Roca-Geronès, Aitor Ramos, Álex Gómez, Sergio Villanueva-Saz, Antonio Fernández, Diana Marteles","doi":"10.1007/s11259-025-10729-5","DOIUrl":"10.1007/s11259-025-10729-5","url":null,"abstract":"<p><p>Ferrets are susceptible to infections with Leishmania infantum, Toxoplasma gondii, and Dirofilaria immitis; however, epidemiological data on these infections are limited in Europe. A total of 448 serum samples from household ferrets were collected between December 2019 and December 2023 in Spain. In this study, we assessed the seroprevalence of L. infantum and D. immitis using an in-house enzyme-linked immunosorbent assay (ELISA) and T. gondii using an in-house immunofluorescence antibody test (IFAT). Among the ferrets tested, the seroprevalence was 10.49% (47/448) for L. infantum, 2.68% (12/448) for T. gondii and 10.27% (46/448) for D. immitis. There was no significant association between seropositivity and age, gender, neutering status, cohabitation, lifestyle, and collection date. Ferrets classified as sick animals related to the presence of compatible or non-compatible signs showed a higher seropositivity rate for L. infantum (15.90%) compared to subclinical animals (4.76%). Overall, 23.44% (105/448) of the samples were seropositive for at least one of the three parasitic agents, 3.12% (15/448) were positive for two agents, and 0.22% (1/448) tested positive for all three agents. Co-infections were also evaluated, revealing that 12.76% (6/47) of L. infantum seropositive ferrets were also positive for T. gondii (p = 0.011) and 21.27% (10/47) for D. immitis (p = 0.009). To the best of our knowledge, this is the first report on the seroprevalence of L. infantum, D. immitis, and T. gondii within the ferret population in Spain. Understanding the epidemiological status of these and other zoonotic pathogens is crucial for enhancing surveillance in both veterinary and public health sectors, as well as for strengthening prevention and control strategies.</p>","PeriodicalId":23690,"journal":{"name":"Veterinary Research Communications","volume":"49 3","pages":"160"},"PeriodicalIF":1.8,"publicationDate":"2025-04-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11976796/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143796467","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impact of single versus multiple infection on serum protein fractions in cats.","authors":"Diana Marteles, María Eugenia Lebrero, Antonio Fernández, Aurora Ortín, Ana González, Carmen Morell, María Jesús Villanueva, Ingo Schäfer, Pablo Quílez, Maite Verde, Alex Gómez, Sergio Villanueva-Saz","doi":"10.1007/s11259-025-10724-w","DOIUrl":"10.1007/s11259-025-10724-w","url":null,"abstract":"<p><p>Serum protein electrophoresis (SPE) is a widely used diagnostic tool for identifying acute and chronic inflammation, as well as immunodeficiencies. However, the impact of co-infections on SPE patterns in cats remains poorly understood. This study explored the utility of SPE in differentiating immune responses between cats infected with a single pathogen and those with multiple co-infections. A total of 79 serum samples from stray European Shorthair cats in Zaragoza, Spain, were analyzed. Fifty cats had a single infection, while 29 were co-infected with 2-4 pathogens. Agarose gel electrophoresis was used to assess protein profiles and statistical analyses were performed to identify significant differences between groups. The results showed no major differences in protein profiles between single and co-infected cats, with polyclonal hypergammaglobulinemia being most common in single-pathogen infections. Therefore, these findings indicate that SPE may have limitations in distinguishing between single and multiple infections in cats, contrasting with some previous studies.</p>","PeriodicalId":23690,"journal":{"name":"Veterinary Research Communications","volume":"49 3","pages":"158"},"PeriodicalIF":1.8,"publicationDate":"2025-04-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11971170/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143781284","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}