Veterinary Research Communications最新文献

{"title":"Enterococcus species identified by MALDI-TOF MS in milk from dairy cow mastitis cases and herd surveys.","authors":"Fernando Ulloa, Martina Penati, Valentina Monistero, Valerio Bronzo, Paolo Moroni, Miguel Salgado, Maria Filippa Addis","doi":"10.1007/s11259-025-10834-5","DOIUrl":"10.1007/s11259-025-10834-5","url":null,"abstract":"<p><p>Enterococcus species are increasingly recognized as mastitis pathogens in dairy cows. Reliable species information is required for correctly defining the regional epidemiology of enterococcal mastitis, establishing its relationships with management variables, and understanding its impact on udder health. We investigated the species distribution of enterococci in bovine milk from subclinical mastitis (SCM) and clinical mastitis (CM) cases and full herd surveys (HS) using MALDI-TOF MS as identification method. A total of 21,864 milk samples from 106 dairy herds were routinely collected and analyzed according to the National Mastitis Council (NMC) guidelines over one year. Enterococcus spp. were found in 4.86% of CM, 5.05% of SCM, and 1.37% of HS milk samples. Overall, E. saccharolyticus was the most prevalent species (55.65%), followed by E. faecium (27.25%), and E. cecorum (5.51%), which showed a significant association with SCM (p-value = 0.0128). This study provides novel data on the Enterococcus species distribution in full herds survey and mastitis cases, highlighting the relevance of MALDI-TOF MS in refining enterococcal mastitis epidemiology, as true prevalence and species distribution may have been skewed by conventional biochemical tests.</p>","PeriodicalId":23690,"journal":{"name":"Veterinary Research Communications","volume":"49 5","pages":"261"},"PeriodicalIF":1.8,"publicationDate":"2025-07-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12287207/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144691739","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Histological and histochemical characterisation of the salivary glands of the palatine fold and the mandibular venom gland of the Komodo dragon (Varanus komodoensis).","authors":"Maciej Janeczek, Karolina Goździewska-Harłajczuk, Agata Małyszek, Ludwika Hrabska, Joanna Klećkowska-Nawrot","doi":"10.1007/s11259-025-10825-6","DOIUrl":"10.1007/s11259-025-10825-6","url":null,"abstract":"<p><p>The Komodo dragon (Varanus komodoensis) is the largest living lizard whose hunting strategy allows it to attack large animals. It inflicts serious damage to its prey with specially developed teeth, called ziphodonts. Although the mandibular venom gland system was previously detected in the Komodo dragon, there is still a lack of its histochemical analysis. Thus, the objective of the current study was a detailed description of the mandibular venom gland of this species. In addition, a histological examination of the salivary glands of the palatine fold was performed. The research material was collected post-mortem from the captive adult female Komodo dragon. Hematoxylin and eosin and Masson-Goldner trichrome staining methods were used for the histological analysis of the glands, while periodic acid-Schiff, alcian blue pH 1.0, alcian blue pH 2.5, alcian blue pH 2.5 PAS and Hale's dialysed iron methods were included for the histochemical study. The venom gland was composed of distinctly marked and very numerous individual lobes surrounded by dense, irregularly structured, highly developed connective tissue that forms the interlobar septa. The salivary glands of the palatine fold were surrounded by a thick connective tissue capsule made of dense, irregularly structured connective tissue. The single ducts of the mandibular venom gland open into the sheaths surrounding the consecutive teeth. The presence of numerous muscle cells in the stroma of the venom gland between its lobes may indicate their participation in the emptying of the vesicles of their secretion.</p>","PeriodicalId":23690,"journal":{"name":"Veterinary Research Communications","volume":"49 5","pages":"260"},"PeriodicalIF":1.8,"publicationDate":"2025-07-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12279577/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144675929","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular analysis supports the morphological identification of the amphistome Pseudodiscus collinsi (Paramphistomoidea: Platyhelminthes) in wild elephants of Sri Lanka.","authors":"R P V J Rajapakse, K J K Karunathilake, T S P Fernando, Huong Thi Thanh Doan, David Blair, Thanh Hoa Le","doi":"10.1007/s11259-025-10827-4","DOIUrl":"https://doi.org/10.1007/s11259-025-10827-4","url":null,"abstract":"<p><p>Post-mortem examinations of elephants in Sri Lanka's Yala National Park morphologically identified amphistome flukes as Pseudodiscus collinsi, a species previously found in Indian elephants. These Sri Lankan worms had 100% ITS-2 sequence identity with that of the Indian P. collinsi Wayanad specimen (GenBank: PQ046280). This species is currently placed in the paramphistomoid family Gastrodiscidae. We compared three markers, ITS-2, partial 28S rDNA (D1-D3 domain), and partial mitochondrial cox1 sequences to previously published data and constructed three maximum likelihood (ML) phylogenies. Across the three phylogenies, the Sri Lankan sequences grouped with other members of the Gastrodiscidae only in the tree inferred from cox1 sequences, albeit with low bootstrap support. The three ML phylogenies revealed inconsistent generic and familial interrelationships. They also suggested that the placement of P. collinsi in the Gastrodiscidae, may need to be reconsidered We report the first record of the amphistome Pseudodiscus collinsi Cobbold, 1875 Sonsino, 1895 (Paramphistomoidea: Platyhelminthes), using morphological and molecular identification, in wild elephants in Sri Lanka.</p>","PeriodicalId":23690,"journal":{"name":"Veterinary Research Communications","volume":"49 5","pages":"259"},"PeriodicalIF":1.8,"publicationDate":"2025-07-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144668636","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The impact of stress in domestic animals: roles of heat shock proteins and acute-phase proteins.","authors":"Tina Yaghoobpour, Zahra Sheikhi, Saeed Nazifi","doi":"10.1007/s11259-025-10802-z","DOIUrl":"https://doi.org/10.1007/s11259-025-10802-z","url":null,"abstract":"<p><p>Stress is a term used to describe environmental stimuli that disrupt homeostasis. In response, animals activate a complex stress response that serves as a defense mechanism to restore balance and maintain physiological function. It impacts growth, reproductive performance, meat quality, animal welfare, and susceptibility to diseases. Heat shock proteins (HSPs) are abundant conserved proteins activated in response to various physiological and environmental stresses, such as heat shock, oxidative stress, heavy metals, ultraviolet radiation, and membrane perturbation Acute phase proteins (APPs) are a recognized method to quantify stressors and serve as reliable biomarkers of animal well-being. Although primarily synthesized in the liver, APPs can also be found in non-hepatic tissues and the bloodstream, reflecting the physiological response to stress. HSPs play a crucial role in the stress response mechanisms of cells, particularly in camels, which adapt to harsh environmental conditions. They help cells adapt by triggering the heat shock response (HSR), increasing the expression of stress-related enhancing homeostasis, and improving stress resistance. Transport stress, particularly in veterinary medicine, can cause organ damage, poor feed conversion ratios, and weight loss in pigs. HSPs detect changes in cellular redox state during stress, prompting other HSPs to normalize misfolded proteins and mitigate cellular stress. Understanding HSPs alterations under stress benefits the animal breeding industry, as animals rapidly synthesize HSPs as a self-protective mechanism. Serum amyloid A (SAA) is another major APP in vertebrates, with increased levels found following inflammation and non-inflammatory conditions. Studies have shown variable results in haptoglobin (Hp) levels in response to various stressors in cattle and other livestock. Understanding the complex responses of different species, stressors, and physiological responses in various animal species is crucial for accurate diagnosis.</p>","PeriodicalId":23690,"journal":{"name":"Veterinary Research Communications","volume":"49 5","pages":"258"},"PeriodicalIF":1.8,"publicationDate":"2025-07-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144650707","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Real-time PCR versus culture of bronchoalveolar lavage fluid for detecting Bordetella bronchiseptica in dogs with persistent lower respiratory signs: a retrospective study of 23 cases.","authors":"S Atencia, N Mateu, A Rodríguez-Cobos, M Parreño, M Labayru, M Molinero, E M González-Alonso-Alegre, A Rodríguez Álvaro, A Caro-Vadillo","doi":"10.1007/s11259-025-10826-5","DOIUrl":"https://doi.org/10.1007/s11259-025-10826-5","url":null,"abstract":"<p><p>Bordetella bronchiseptica (Bb) is a frequent pathogen in dogs and a component of the Canine Infectious Respiratory Disease Complex (CIRDC). However, it can also be detected in the lower respiratory tract of healthy animals, complicating its interpretation in clinical settings. This retrospective study compared the detection of Bb in bronchoalveolar lavage fluid (BALF) samples using real-time PCR versus bacterial culture in dogs referred for persistent lower respiratory tract signs to a referral hospital in Spain between 2018 and 2024. A total of 143 BALF samples were analyzed; 23 dogs tested positive for Bb by at least one method and were included in the clinical analysis. Real-time PCR identified Bb in 16.1% (23/143) of cases (95% CI:10.4-23.1%). Among these, 40% (9/23) had negative culture results. Co-infections with Mycoplasma spp. or Mycoplasma cynos were detected in 30% (7/23) of cases, and 26% (6/23) had positive cultures for other bacteria. Most dogs (91% (21/23)) had received antibiotics prior to BALF collection. The median time to clinical remission was 30 days. While real-time PCR showed a higher detection rate than culture, its results must be interpreted cautiously. PCR may amplify DNA from non-viable organisms or colonizers, and thus does not confirm active infection. Our findings underscore the need to integrate molecular, microbiological, and clinical data to reach an accurate diagnosis and avoid inappropriate antimicrobial use. Combining PCR and culture may enhance diagnostic accuracy, as culture alone would have failed to detect Bb in 40% of PCR positive dogs.</p>","PeriodicalId":23690,"journal":{"name":"Veterinary Research Communications","volume":"49 5","pages":"257"},"PeriodicalIF":1.8,"publicationDate":"2025-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144638202","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Establishment of an ELISA for the detection of antibodies against Stenotrophomonas maltophilia.","authors":"Qian Zhang, Lu Dai, Fuxian Zhang, Zijie Xu, Xiangrui Yang, Hong Chu, Guangmou Yan, Na Li, Fengyang Li, Liancheng Lei","doi":"10.1007/s11259-025-10828-3","DOIUrl":"https://doi.org/10.1007/s11259-025-10828-3","url":null,"abstract":"<p><strong>Background: </strong>Stenotrophomonas maltophilia (S. maltophilia) is a conditionally pathogenic bacterium around the world. In humans, it mainly harms patients with immunodeficient or chronic diseases, leading to high mortality rate of patients with pneumonia and bacteremia. In the veterinary medicine, it caused respiratory infections or mixed infections with other common pathogens in pigs. However, infection of S. maltophilia has been overlooked and the prevalence of S. maltophilia in porcine remains unknown due to a lack of diagnosis method.</p><p><strong>Methods: </strong>In this study, an indirect ELISA was established using the purified Phospholipase C (PLC) protein of S. maltophilia SMFZ-01 strain as the coating antigen. Checkerboard titration was performed for the optimization of the coating concentration of the recombinant PLC (rPLC) protein, the dilution and incubation time of serum and the IgG-HRP secondary antibody. The specificity, repeatability and sensitivity of established ELISA was also optimized. The positive rate of S. maltophilia antibodies was calculated using 305 clinical porcine sera samples by either established indirect ELISA or a nested PCR.</p><p><strong>Results: </strong>rPLC was successfully purified and identified by Western blot. The optimal coating concentration of rPLC was 2.5 µg/mL. The optimal dilutions of serum samples and secondary antibody were 1:400 and 1:2500, respectively. The analytical sensitivity was 1:1600, with no cross-reaction with the positive sera of other common porcine pathogens. The intra-assay and inter-assay reproducibility coefficients of variation was less than 10%. Detection of 305 clinical porcine sera samples revealed a high positive rate by established ELISA (12.01%) and nested PCR (29.87%).</p><p><strong>Conclusions: </strong>To our knowledge, this is the first study to develop an indirect ELISA for the detection and epidemiological analysis of S. maltophilia in porcine. Our findings indicate that S. maltophilia may be more prevalent in porcine populations than previously recognized, though further studies are needed to confirm this. The established indirect ELISA enhances our understanding of S. maltophilia infections in pig herds and provides valuable insights for designing future prevention and control strategies, along with considerations regarding the health and welfare of porcine.</p>","PeriodicalId":23690,"journal":{"name":"Veterinary Research Communications","volume":"49 5","pages":"256"},"PeriodicalIF":1.8,"publicationDate":"2025-07-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144620772","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cerebrospinal fluid estradiol fluctuations during the estrous cycle and their association with intraocular pressure and optic nerve sheath diameter in dogs: a first report.","authors":"Candemir Özcan, Serkan Bozacı, Berrak Işık Soytürk, Kenan Çağrı Tümer, Ömer Deniz, Tarık Şafak, Ayşe Başak Dellalbaşı, Özgür Kaynar, Elif Dogan","doi":"10.1007/s11259-025-10820-x","DOIUrl":"10.1007/s11259-025-10820-x","url":null,"abstract":"<p><strong>Objective: </strong>The relationship between optic nerve sheath diameter (ONSD), an indicator of intracranial pressure (ICP), and intraocular pressure (IOP) is controversial. The two aims of this study were; first, to investigate the effect of cerebrospinal fluid (CSF) estradiol (E2) and progesterone (P4) levels on ONSD and second, to investigate the relationship between ONSD and IOP in female dogs.</p><p><strong>Methods: </strong>IOP measurements were performed using rebound tonometer, and transpalpebral ultrasonographic assessment of the ONSD was conducted. CSF samples were collected, and E2 and P4 levels were quantified using enzyme-linked immunosorbent assay (ELISA). Multiple regression analysis was conducted to assess the relationships among the variables.</p><p><strong>Results: </strong>Multiple regression analyses on dogs found that the level of cerebrospinal fluid estradiol level (CSF-E2) significantly influenced left (L) ONSD (p = 0.0421, R² = 0.1551) and right (R) ONSD (p = 0.0216, R² = 0.1938). Systolic blood pressure (SBP) was a significant independent variable for left intraocular pressure (LIOP) (p = 0.0122, R² = 0.2261), while body weight was a significant predictor for right intraocular pressure (RIOP) (p = 0.0008, R² = 0.3679).</p><p><strong>Conclusions: </strong>This study revealed that CSF-E2 levels exert a significant influence on ONSD in dogs, whereas no direct association was observed between IOP and ONSD. These findings underscore the notion that ONSD is modulated not only by hemodynamic mechanisms but also by hormonal regulation.</p>","PeriodicalId":23690,"journal":{"name":"Veterinary Research Communications","volume":"49 5","pages":"255"},"PeriodicalIF":1.8,"publicationDate":"2025-07-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12254181/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144609735","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Immunomodulatory and antioxidant responses of African catfish (Clarias gariepinus) to cestode infections: implications for intestinal health and aquaculture management.","authors":"Mahmoud Radwan, Jamila S Al Malki, Amaal Mohammadein, Mohamed Fares","doi":"10.1007/s11259-025-10819-4","DOIUrl":"https://doi.org/10.1007/s11259-025-10819-4","url":null,"abstract":"<p><p>Inadequate information exists on host-tapeworm parasite interactions in African catfish (Clarias gariepinus, Burchell 1822). Hence, this study evaluated the natural C. gariepinus severely infected mildly infected, on histopathological features, intestine health, immunological and antioxidant response to cestode parasites infection (Polyonchobothrium clarias (Woodland, 1925) Meggitt, 1930, Proteocephalus sp.). The prevalence of cestodes was 80%, with 26 and 52% of these fish mildly and severely infected, respectively. The prevalence was positively correlated with turbidity (0.64), NO₂ (0.58), NO₃ (0.63), and total alkalinity (0.57). Data illuminated that mildly and severely infected fish have more harmful effects on intestinal health and hemato-biochemical biomarkers than uninfected fish. Liver and intestinal antioxidant indices showed that the infected fish had higher malondialdehyde (MDA) levels than the uninfected fish. Correspondingly, mildly infected groups' liver and intestine showed significantly higher levels of superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GSH), while severely infected groups showed lower levels. Data clarified that infected fish have significantly upregulated pro-inflammatory genes IL-1β, Tlr1, and C3 expression, especially in mildly infected, than uninfected fish. However, the expression of CAT and SOD genes was significantly downregulated, in the severely infected, along with higher expression of the HSP70 gene than those of the uninfected groups. The histopathological study revealed that fish infected with the cestode parasite had inflammatory and abnormal features, especially in severely infected fish groups. Hence, the study concluded that the cestode parasites induce pathological immunological responses and health status in C. gariepinus.</p>","PeriodicalId":23690,"journal":{"name":"Veterinary Research Communications","volume":"49 5","pages":"254"},"PeriodicalIF":1.8,"publicationDate":"2025-07-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144609736","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correction to: Effects of central injection of liver-expressed antimicrobial peptide-2 (LEAP2) on feed intake in broiler chickens: interactions with opioidergic and serotonergic systems.","authors":"Kowsar Saffar, Morteza Zendehdel, Bita Vazir, Shahin Hassanpour, Kimia Mahdavi","doi":"10.1007/s11259-025-10824-7","DOIUrl":"https://doi.org/10.1007/s11259-025-10824-7","url":null,"abstract":"","PeriodicalId":23690,"journal":{"name":"Veterinary Research Communications","volume":"49 5","pages":"253"},"PeriodicalIF":1.8,"publicationDate":"2025-07-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144601674","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular epidemiology and phylogenetic analysis of Echinococcus spp. in the Qinghai-Tibetan Plateau: unraveling genetic evolution in a high-risk region for echinococcosis.","authors":"Guoyan Zhou, Zhi Li, Xueyong Zhang, Ru Meng, Xiuying Shen, Zhen Zhang, Fuyun Chen, Hong Duo, Yong Fu","doi":"10.1007/s11259-025-10815-8","DOIUrl":"https://doi.org/10.1007/s11259-025-10815-8","url":null,"abstract":"<p><p>As the largest and highest plateau in the world, the Qinghai-Tibetan Plateau (QTP) also represents a critical endemic region for echinococcosis, a zoonotic disease caused by Echinococcus spp. The disease is listed as a neglected tropical disease by the World Health Organization (WHO). Given its high prevalence and associated socioeconomic impacts, there is an urgent need to conduct in-depth research on this problem and formulate prevention and control intervention strategies. Here, we conducted a molecular genetic analysis of 1299 samples (including canid feces and livestock hydatid cysts) from the QTP based on the mitochondrial cytochrome c oxidase I (COI) gene. Our results revealed the infection rate of echinococcosis had some regional differences and identified 24 distinct haplotypes that clustered into five Echinococcus species. Encouragingly, the first isolation and identification of Echinococcus equinus in Ovis aries laid the foundation for the cross-species transmission of Echinococcus. Phylogenetic analyses (ML/BI) consistently supported highly clustering of each haplotype with its reference sequence (bootstrap ≥ 80%, PP ≥ 0.80). Furthermore, molecular clock estimates indicated that the co-evolution of Echinococcus spp. and its host may be closely related to the rapid uplift and climate changes of the QTP since the Cenozoic era. This study provided the latest dataset on Echinococcus infections in natural hosts across the QTP, systematically revealing the epidemic characteristics, genotype distribution and genetic diversity of Echinococcus spp. in this region.</p>","PeriodicalId":23690,"journal":{"name":"Veterinary Research Communications","volume":"49 5","pages":"252"},"PeriodicalIF":1.8,"publicationDate":"2025-07-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144601675","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}