Veterinary Research Communications最新文献

{"title":"Outbreak of subacute necrotic myopathy caused by ionophore poisoning in water buffaloes (Bubalus bubalis).","authors":"Fernanda Felicetti Perosa, Anderson Hentz Gris, Luan Cleber Henker, Jean Carlo Olivo Menegatt, Paola Sônego, José Tiago Roza de Moraes, Saulo Petinatti Pavarini, David Driemeier, Welden Panziera","doi":"10.1007/s11259-025-10749-1","DOIUrl":"https://doi.org/10.1007/s11259-025-10749-1","url":null,"abstract":"<p><p>This study reports an outbreak of subacute necrotic myopathy in buffaloes associated with the ingestion of a commercial feed formulated for cattle containing monensin. Twenty-nine buffaloes were fed a monensin containing ration, and after two weeks, nine animals developed apathy, anorexia, dark-red urine, recumbency, and ventral abdominal distention. Four of them died and one was euthanized in extremis within two to five days after the onset of clinical signs. Three buffaloes were necropsied, and the main lesions included focally extensive areas of pallor, mainly in the musculature of the thoracic and pelvic limbs. Upon histological examination, these areas were characterized by polyphasic myonecrosis, with floccular and hyaline necrosis of myocytes associated with marked inflammatory infiltration of lymphocytes, plasma cells, and macrophages, as well as areas with proliferation of fibrous connective tissue, proliferation of satellite cells, and occasional mineralization of necrotic fibers. Similar areas of myonecrosis were observed in the heart in the three cases. The history of ingestion of feed containing monensin formulated for cattle and the absence of alternative causes for myonecrosis supported the diagnosis of ionophore poisoning in buffaloes in this report. The subacute pattern of myonecrosis observed in this outbreak is uncommonly reported in buffaloes and it was associated with the prolonged period of ingestion (presumably below toxic levels) of the monensin-containing feed.</p>","PeriodicalId":23690,"journal":{"name":"Veterinary Research Communications","volume":"49 3","pages":"178"},"PeriodicalIF":1.8,"publicationDate":"2025-04-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144017956","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bergeyella zoohelcum: A first case report of its association with respiratory diseases in swine in China.","authors":"Zenghai Jiang, Muhammad Umar Yaqoob, Abubakar Siddique, Juheng Guang, Abdelaziz Ed-Dra, Min Yue","doi":"10.1007/s11259-025-10735-7","DOIUrl":"https://doi.org/10.1007/s11259-025-10735-7","url":null,"abstract":"<p><p>The current investigation presents the first case report of Bergeyella zoohelcum associated with respiratory tract infections in pigs in China. Two Gram-negative bacterial isolates with bacillus morphology were isolated from the heart and lung samples of two pigs having respiratory symptoms in two separate cases. The isolates were identified as B. zoohelcum using morphological, biochemical, and molecular approaches, including 16 S rRNA sequencing. Antimicrobial susceptibility test (AST) revealed that the B. zoohelcum isolates exhibit multi-drug resistance (MDR) patterns, particularly the B. zoohelcum ZWSF strain, which showed resistance to several antibiotics including polymyxin B, tetracycline, chloramphenicol, erythromycin, fosfomycin, amikacin, and gentamicin. Therefore, further investigations are necessary to better understand the pathogenicity of B. zoohelcum in swine and to prevent its spread.</p>","PeriodicalId":23690,"journal":{"name":"Veterinary Research Communications","volume":"49 3","pages":"176"},"PeriodicalIF":1.8,"publicationDate":"2025-04-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144037107","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification of miR-71* as a regulator of Flotillin-1 in egg development of Rhipicephalus sanguineus.","authors":"Qilin Wang, Muxiao Li, Wenge Liu, Yanlong Wang, Zeyu Chen, Zhenjie Zhang, Guangyuan Liu, Meng Qi","doi":"10.1007/s11259-025-10727-7","DOIUrl":"https://doi.org/10.1007/s11259-025-10727-7","url":null,"abstract":"<p><p>Identifying the microRNAs (miRNAs) that regulate the growth and development of Rhipicephalus sanguineus (Rh. sanguineus) is essential for developing effective control strategies against both the tick and tick-borne pathogens. This study reveals the antagonistic regulatory mechanism between miR-71* (the passenger strand of miRNA-71) and Flotillin-1 in the egg development of Rh. sanguineus. Through bioinformatics prediction and validation using a dual-luciferase reporter system, we demonstrate for the first time that miR-71* directly targets and regulates Flotillin-1. Functional experiments indicate that inhibiting miR-71* significantly increases tick egg weight, while suppressing Flotillin-1 reduces egg weight, thereby suggesting that these molecules negatively regulate egg developmental processes. This discovery provides a novel target for elucidating reproductive regulatory pathways in ticks and lays a theoretical foundation for developing control strategies against ticks and tick-borne pathogens.</p>","PeriodicalId":23690,"journal":{"name":"Veterinary Research Communications","volume":"49 3","pages":"172"},"PeriodicalIF":1.8,"publicationDate":"2025-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143987484","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Serodiagnosis of equine infectious anemia by indirect ELISA based on a novel synthetic peptide derived from gp45 glycoprotein.","authors":"Aníbal Domínguez-Odio, Laura Iliana Coroas González, Dayamí Martín Alfonso, Francisco Guevara-Hernández, Manuel Alejandro La O Arias, Mayelin Paneque Zayas, Miguel Ángel Bedoya Ríos","doi":"10.1007/s11259-025-10707-x","DOIUrl":"https://doi.org/10.1007/s11259-025-10707-x","url":null,"abstract":"<p><p>The transmembrane glycoprotein gp45 (implicated in virus-equine B lymphocyte binding and in subsequent infection) is an important protein used as an antigen in the serological diagnosis of equine infectious anemia, but synthetic peptides derived from its structure are not. Consequently, the present study aimed to determine the diagnostic performance of the Bio-AIELAB immunoassay (novel synthetic peptide P05/gp45) in comparison with a similar commercial assay (ELISA EIA, VMRD Inc, USA) and with the gold standard (AGID, LABIOFAM, Cuba). A total of 365 samples of sera from naturally infected Cuban horses (positive: 179, and negative: 186) were investigated by Bio-AIELAB and AGID, of which 299 (positive: 147, and negative: 152) were analysed by ELISA EIA. The performance indicators used in all cases were: relative diagnostic sensitivity and specificity, positive and negative predictive values, as well as concordance according to the Kappa index. The Bio-AIELAB system obtained the best performance in sensitivity (99.44%, 178/179), specificity (95.16%, 177/186) and concordance (Kappa index: 0.94) with respect to ELISA EIA test. Based on the above elements, Bio-AIELAB could be a promising candidate for use in equine infectious anemia surveillance programs in combination with the AGID test.</p>","PeriodicalId":23690,"journal":{"name":"Veterinary Research Communications","volume":"49 3","pages":"174"},"PeriodicalIF":1.8,"publicationDate":"2025-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12014826/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144041354","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"An integrated in silico and ex vivo study identifies quinazolinedione L134716 as a potential inhibitor of infectious bronchitis virus.","authors":"Irfan Gul, Ishara M Isham, Shahnas M Najimudeen, Amreena Hassan, Ehtishamul Haq, Riaz Ahmad Shah, Nazir Ahmad Ganai, Syed Mudasir Ahmad, Naveed Anjum Chikan, Mohamed Faizal Abdul-Careem, Nadeem Shabir","doi":"10.1007/s11259-025-10742-8","DOIUrl":"https://doi.org/10.1007/s11259-025-10742-8","url":null,"abstract":"<p><p>Infectious Bronchitis Virus (IBV) poses a persistent threat to poultry health and productivity, resulting in substantial economic losses. Despite the deployment of live attenuated and inactivated vaccines, effective control of IBV remains challenging, emphasizing the need for alternative strategies to manage infections. This study identifies dual inhibitors targeting the main protease (Mpro) and papain-like protease (PLpro) of infectious bronchitis virus (IBV) using a combinatorial in silico and ex vivo approach. Screening of the MyriaScreen Diversity Library II, comprising 10,000 diverse small molecules, resulted in the selection of two promising compounds, ST092577 and L134716, based on their strong and stable interactions with both proteases. Molecular dynamics (MD) simulations further confirmed the stability of these complexes, with their binding interactions validated through MM-PBSA binding free energy calculations. Ex vivo validation utilizing tracheal organ cultures and quantitative PCR demonstrated that 50 µM of L134716 (4-(4-(benzyloxy)ph)-7,7-dimethyl-4,6,7,8-tetrahydro-2,5(1 H,3 H)-quinazolinedione) significantly reduced the IBV genome load in infected tracheal rings. This reduction in viral load was further corroborated by immunohistochemical analysis. These findings underscore the promising potential of targeting key viral proteases Mpro and PLpro as part of alternative therapeutic strategies against IBV infections in poultry. While the results are encouraging, additional in ovo and in vivo studies are necessary to validate these findings and further explore the efficacy of L134716 in practical applications.</p>","PeriodicalId":23690,"journal":{"name":"Veterinary Research Communications","volume":"49 3","pages":"175"},"PeriodicalIF":1.8,"publicationDate":"2025-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144016546","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Infectivity and persistence of bovine leukemia virus in human breast cells: assessing a possible zoonotic link to cancer.","authors":"Pedro Edgardo Moran, María Victoria Nieto Farías, Guillermina Laura Dolcini, María Carolina Ceriani","doi":"10.1007/s11259-025-10738-4","DOIUrl":"https://doi.org/10.1007/s11259-025-10738-4","url":null,"abstract":"<p><p>The study analyzes the potential link between bovine leukemia virus (BLV), an oncogenic retrovirus in cattle, and human breast cancer. BLV infects lymphocytes in cattle, causing persistent infections; while most animals are asymptomatic, some develop lymphosarcoma. The detection of BLV proviral DNA in milk and meat, along with antibodies and proviral DNA in humans, suggests possible zoonotic transmission. In controlled conditions, human mammary epithelial cells (MCF-10 A) were infected with BLV, and the effect of 17-β estradiol (E2) was assessed as a model for natural exposure. BLV integrated its DNA into human cells, increasing the proviral load in the presence of E2, suggesting E2 enhances viral integration. The virus replicated and produced infectious particles capable of infecting bovine mammary cells (MAC-T). Findings suggest BLV could establish infections in human mammary cells under certain conditions, potentially contributing to cancer development. The study highlights the need for further research into its zoonotic potential and emphasizes the importance of food safety practices, particularly regarding raw milk consumption.</p>","PeriodicalId":23690,"journal":{"name":"Veterinary Research Communications","volume":"49 3","pages":"173"},"PeriodicalIF":1.8,"publicationDate":"2025-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144017591","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Feline gastrointestinal eosinophilic sclerosing fibroplasia in a young ragdoll cat: with surgical management.","authors":"Jindong Gao, Yuning Li, Yang Xie, Md F Rahim, Md F Kulyar, Quan Mo, Jianzhong He, Mengdi Zhang, Xing Wu, Jinhua Yin","doi":"10.1007/s11259-025-10740-w","DOIUrl":"https://doi.org/10.1007/s11259-025-10740-w","url":null,"abstract":"<p><p>Feline gastrointestinal eosinophilic sclerosing fibrosis (FGESF) is a rare condition mainly affecting middle-aged cats. It is characterized by inflammatory fibrous masses in the gastrointestinal tract. In this case study, we present the case of a 5-year-old neutered Ragdoll cat who experienced chronic constipation, weight loss, and anorexia. A narrow rectal lumen with a dorsal mass was observed during the physical examination, along with tachycardia, tachypnea, and poor body condition. Hematological analysis revealed peripheral eosinophilia, while biochemical examination showed elevated levels of immunoglobulin and feline serum amyloid, indicating the presence of inflammation. Radiography and ultrasound confirmed the presence of a sub-lumbar mass compressing the rectum and urethra. The endoscopic evaluation revealed smooth rectal mucosa without any lesions. During surgical exploration, firm, spherical masses were found in the sub lumbar region, indicating lymphadenopathy. Multiple biopsy samples were taken for cytological and histopathological analysis, which confirmed lymphadenitis with eosinophilic and sclerotic changes. Antimicrobial susceptibility testing identified the presence of Pseudomonas aeruginosa. Surgical excision of the masses was performed, followed by postoperative management with ceftiofur, lactulose, and antibiotics. Cytological examination showed inflammatory cell infiltration without any signs of malignancy. Histopathology revealed dense fibrous hyperplasia with reactive lymphoid hyperplasia. The discussion focuses on differential diagnoses, pathogenesis, and treatment options. Although immunosuppressants are commonly used, surgical excision and antibiotics were chosen as the treatment approach in this case. The unique presentation of a younger Ragdoll cat and the successful surgical outcome highlights the importance of being vigilant in diagnosing and managing FGESF. In the future, it would be beneficial to consider preoperative cytology to guide treatment decisions and explore alternative therapeutic approaches.</p>","PeriodicalId":23690,"journal":{"name":"Veterinary Research Communications","volume":"49 3","pages":"170"},"PeriodicalIF":1.8,"publicationDate":"2025-04-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144030207","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Assessment of macronutrients and fat quality in cat food.","authors":"Jagoda Kępińska-Pacelik, Wioletta Biel, Patrycja Biernacka, Grzegorz Tokarczyk, Grzegorz Bienkiewicz","doi":"10.1007/s11259-025-10741-9","DOIUrl":"https://doi.org/10.1007/s11259-025-10741-9","url":null,"abstract":"<p><p>For certain nutrients, the European Pet Food Industry Federation (FEDIAF) provides minimum recommended levels that should be met in a cat's diet. The aim of this study was to assess the levels of essential nutrients and the quality of fats in commercial cat food. The material analyzed consisted of 27 dry cat food products, which were categorized into three groups based on their primary protein sources: poultry, fish, and alternative proteins. The proximate composition of ground samples was determined according to methods established by the Association of Official Analytical Chemists (AOAC). Fatty acid methyl esters were extracted from the samples and analyzed using gas chromatography coupled with mass spectrometry. All analyzed cat foods met the minimum recommended levels for protein and fat for adult cats, as established by FEDIAF (2024b). However, 11% of the products did not meet the minimum recommended level for linoleic acid (C18:2 n-6), and 14% lacked arachidonic acid (C20:4). The analysis confirmed that cat foods containing different types of animal-derived ingredients vary significantly in their individual fatty acid profiles.</p>","PeriodicalId":23690,"journal":{"name":"Veterinary Research Communications","volume":"49 3","pages":"171"},"PeriodicalIF":1.8,"publicationDate":"2025-04-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144017145","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"New records of fleas and ticks (Siphonaptera and Ixodida) parasitizing wild mammals in Mossoró Municipality, Rio Grande do Norte State, Brazil.","authors":"Ícaro Costa Moreira, Ana Caroline Freitas Caetano de Sousa, Luana Priscila Gomes Cândido, Maria Danielle Chagas Dos Santos, Ana Beatriz Pinheiro Guerra, Carlos Iberê Alves Freitas, Moacir Franco de Oliveira, Thiago Fernandes Martins, Ricardo Bassini-Silva, Josivania Soares Pereira","doi":"10.1007/s11259-025-10743-7","DOIUrl":"https://doi.org/10.1007/s11259-025-10743-7","url":null,"abstract":"<p><p>The Caatinga is a Brazilian biome with limited studies on its wild mammal fauna, including the ectoparasitic relationships in these animals. This study aims to report the findings of fleas and ticks infesting wild animals rescued in the municipality of Mossoró, Rio Grande do Norte State (RN), Brazil, and treated at the Veterinary Hospital of the Universidade Federal Rural do Semi-Árido (HOVET-UFERSA). During the clinical assessments and physical examinations of the host animals (one male Cerdocyon thous L.; two females of Tamandua tetradactyla L.; one male Leopardus tigrinus Schreber; and one male Conepatus semistriatus Boddaert), fleas and ticks were collected. These ectoparasites were removed mechanically and sent to the Animal Parasitology Laboratory at UFERSA for identification. Identification was carried out based on morphology, with the aid of specific literature, stereomicroscopy, and light optical microscopy. From C. thous, two males of Amblyomma parvum Aragão and four female Pulex irritans (L.) fleas were recovered. From the adult female T. tetradactyla, one male of Rhipicephalus linnaei (Audouin), eight nymphs, and two males of Amblyomma auricularium (Conil) were collected. From the juvenile female T. tetradactyla, three nymphs of A. parvum and six female fleas of the species Tunga penetrans (L.) were recovered. From the male L. tigrinus, five females and one male flea of the species Ctenocephalides felis (Bouche) were collected. Finally, from the male C. semistriatus, two nymphs, 19 males, and six females of A. auricularium were recovered. This study contributes to expanding the knowledge of host-parasite relationships in free-living mammals in the Caatinga biome, in western Rio Grande do Norte.</p>","PeriodicalId":23690,"journal":{"name":"Veterinary Research Communications","volume":"49 3","pages":"169"},"PeriodicalIF":1.8,"publicationDate":"2025-04-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144016556","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Residual depletion of eugenol as an anesthetic in growing phase pacu (Piaractus mesopotamicus).","authors":"Caio Francisco Santana Farias, Jayme Aparecido Povh, Ruy Alberto Caetano Corrêa Filho, Andrea Maria de Araújo Gabriel, Claudia Andrea Lima Cardoso, Adryadine Almeida da Costa, Antônio Francisco Oliveira, Gabriela Tomas Jerônimo, Maurício Laterça Martins, Arlene Sobrinho Ventura","doi":"10.1007/s11259-025-10739-3","DOIUrl":"https://doi.org/10.1007/s11259-025-10739-3","url":null,"abstract":"<p><p>The aim of this study was to evaluate the pharmacokinetics of eugenol in pacu (Piaractus mesopotamicus) juveniles following exposure to an immersion bath. Pacu juveniles (n = 42) with an average weight of 546.40 ± 74.51 g and total length of 25.43 ± 1.52 cm were subjected to a bath with 100 mg L^-1 eugenol previously dissolved in 95% ethanol (1:10). After a 10-min immersion, the fish were transferred to anesthetic-free aquaria for recovery. Blood, kidney, muscle, spleen, gills, and brain samples were collected at intervals 0, 0.5, 1.0, 3.0, 6.0, 12.0, and 24.0 h post-anesthesia. Pharmacokinetic parameters were calculated using non-compartmental methods. The highest eugenol concentrations were recorded immediately after anesthesia (0 h) in all tissues, in descending order: plasma > kidney > muscle > spleen > gills > brain. The plasma and brain half-lives of eugenol were 1.12 h and 0.73 h, respectively, with elimination constants of 0.62 and 0.95 per hour. In muscle and kidney tissues, the half-lives were 0.14 h and 0.09 h, with elimination constants of 4.97 and 7.44 per hour, respectively. In the spleen and gills, the half-lives were 0.21 h and 0.23 h, with elimination constants of 3.26 and 3.07 per hour. In conclusion, eugenol is rapidly absorbed and eliminated in growing phase pacu, reaching peak concentrations soon after anesthesia and showing a significant reduction up to 24 h.</p>","PeriodicalId":23690,"journal":{"name":"Veterinary Research Communications","volume":"49 3","pages":"168"},"PeriodicalIF":1.8,"publicationDate":"2025-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144021012","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}