Veterinary Research Communications最新文献

{"title":"Antimicrobial resistance and biofilm production by Staphylococcus spp. and Mammaliicoccus Sciuri in Inia araguaiaensis: surveillance under a one health perspective in the Amazon.","authors":"Denny Parente de Sá Barreto Maia Leite, Pollyanne Raysa Fernandes de Oliveira, Flavia Cristina Matos Oliveira, Gabriel Melo-Santos, Guilherme Maricato, Karolina Rosa Fernandes Beraldo, Angélica Lúcia Figueiredo Rodrigues, Alexsandro Antonio Portilho Damasceno, Rodrigo Hipólito Tardin Oliveira, Thais Fernanda Campos Fraga-Silva, Maria Aparecida Juliano, Frederico Ozanan Barros Monteiro, Abelardo Silva-Júnior, Rinaldo Aparecido Mota","doi":"10.1007/s11259-025-10836-3","DOIUrl":"10.1007/s11259-025-10836-3","url":null,"abstract":"<p><p>Antimicrobial resistance in natural environments poses an increasing threat to public health and biodiversity conservation. This study investigated antimicrobial resistance and biofilm formation in bacteria isolated from Inia araguaiaensis, an Amazonian endemic dolphin species. Skin lesion samples were collected from eight individuals in the Mocajuba region, Pará, Brazil. Bacterial isolation was performed on Brain Heart Infusion agar and Mannitol Salt agar. Species identification was carried out using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS), and antimicrobial susceptibility testing was conducted by disk diffusion following. Resistance genes were detected by polymerase chain reaction, and biofilm formation was assessed using the crystal violet staining method. A total of 19 bacterial isolates were recovered, including Staphylococcus spp. (n = 14) and Mammaliicoccus sciuri (n = 5). The most frequently identified species were Staphylococcus aureus (4/19; 21.1%), S. warneri (4/19; 21.1%), and S. epidermidis (4/19; 21.1%). A high prevalence of penicillin resistance was observed (18/19; 94.7%), followed by resistance to erythromycin, tetracycline, and sulfamethoxazole-trimethoprim (each 4/19; 21.1%), and gentamicin (3/19; 15.8%). Four isolates (4/19; 21.1%) exhibited a multidrug-resistant (MDR) profile. Genotypic screening revealed the presence of the blaZ gene in 8/19 isolates (44.4%), followed by mecA, msrA, and norC (each 2/19; 10.5%). The tetracycline resistance genes tetL and tetM were detected in 3/19 (15.8%) and 1/19 (5.3%) isolates, respectively. Biofilm formation was detected on 15/19 (78.9%) of the isolates, of which 4/15 (26.7%) were classified as strong producers, 1/15 (6.7%) as moderate, and 10/15 (66.7%) as weak producers. These findings support the recognition of river dolphins as potential sentinels for antimicrobial resistance in aquatic ecosystems and reinforce the importance of integrating microbiological monitoring into broader conservation efforts under the One Health perspective.</p>","PeriodicalId":23690,"journal":{"name":"Veterinary Research Communications","volume":"49 5","pages":"271"},"PeriodicalIF":2.0,"publicationDate":"2025-08-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144785439","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Metabolomic evidence of bovine leukemia virus regulation on ferroptosis in bovine mammary epithelial cells.","authors":"Shuai Lian, Xiujuan Zheng, Miao Yu, Zijian Geng, Jianfa Wang, Guanxin Lv, Di Wang","doi":"10.1007/s11259-025-10840-7","DOIUrl":"https://doi.org/10.1007/s11259-025-10840-7","url":null,"abstract":"<p><p>Bovine leukemia virus (BLV) is a globally prevalent pathogen that can cause enzootic bovine leukosis (EBL), which reduces dairy cows' immunity, interferes with mammary antimicrobial defense capabilities, and exacerbates clinical mastitis. However, the specific mechanisms by which these effects occur remain incompletely understood. Ferroptosis, an iron-dependent non-apoptotic cell death mechanism, exhibits dual roles in viral infections. In this study, wild-type BLV and a miRNA-deficient mutant strain (BLV-ΔmiRNA) were used to infect bovine mammary epithelial cells in vitro, combined with non-targeted metabolomics to investigate BLV-regulated ferroptosis evidence. The results shown that BLV significantly elevated levels of unsaturated fatty acid, interferes with vitamin B6 metabolism. Suggest that BLV promote ferroptosis in mammary epithelial cells through unsaturated fatty acid biosynthesis and vitamin B6 metabolism pathways, potentially involving BLV-encoded miRNAs. This research provides a theoretical foundation for identifying novel BLV pathogenic targets and prevention strategies.</p>","PeriodicalId":23690,"journal":{"name":"Veterinary Research Communications","volume":"49 5","pages":"270"},"PeriodicalIF":2.0,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144761537","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Emerging GI-23 infectious bronchitis virus in vaccinated broiler chickens: detection and molecular characterization during a respiratory outbreak in Southern Brazil.","authors":"Graziela Borges Dos Santos, Carolina Isabela Mucellini, Luiz Almiro Carvalho Sesti, Ruy Diego Chacón, Jorge Luis Chacón, Elisabete Takiuchi","doi":"10.1007/s11259-025-10838-1","DOIUrl":"10.1007/s11259-025-10838-1","url":null,"abstract":"<p><p>Infectious Bronchitis Virus (IBV) is an avian coronavirus that significantly impacts poultry production worldwide. Analysis of the complete S1 gene has classified IBV into genotypes GI to GIX and more than 40 lineages to date. This study aimed to investigate whether a new IBV variant was involved in respiratory disease outbreaks observed in 2021 in vaccinated commercial broiler flocks in Paraná State, Brazil. Day-old chicks in all affected flocks had received mass IBV vaccination at the hatchery with a combination of two live-attenuated vaccines, specifically the predominant lineages GI-1 (Mass-type) and GI-11 (BR-I). Organ samples (air sac, trachea, lung, cecal tonsils, and kidney) were collected from twelve commercial broiler flocks across four poultry companies. Semi-nested RT-PCR targeting the HVR3 region detected IBV in all flocks, with 80.4% (37/46) of samples testing positive. IBV was detected in all types of organs studied, with a 100% detection rate in kidneys and trachea, and 90.9% in cecal tonsils. Eighteen amplicons, representing samples from all flocks, and one derived from the commercial vaccine containing IBV Var-2 strain were sequenced. The nucleotide identities ranged from 99.2 to 100% among them and demonstrated high homology with IBV Var-2 strain sequences (GI-23 lineage), prevalent in the Middle East and Europe. Phylogenetic analysis based on HVR3 clustered the Brazilian field strains within subclade GI-23.1. A second phylogenetic reconstruction using full-length S1 sequences showed the same topology, indicating that HVR3-based classification provided satisfactory resolution for lineage identification in the context of initial molecular surveillance efforts. The detection of the exotic GI-23 lineage underscores the importance of continued surveillance of circulating IBV strains to refine disease control strategies.</p>","PeriodicalId":23690,"journal":{"name":"Veterinary Research Communications","volume":"49 5","pages":"269"},"PeriodicalIF":2.0,"publicationDate":"2025-07-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144754424","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Use of type I bovine collagen membranes for treating deep and perforating corneal ulcers in brachycephalic dogs: a case series.","authors":"Sandra P Acevedo, Nathalia M Correa-Valencia","doi":"10.1007/s11259-025-10829-2","DOIUrl":"https://doi.org/10.1007/s11259-025-10829-2","url":null,"abstract":"<p><p>This retrospective study aimed to assess the effectiveness of lamellar keratoplasty using 200 μm type I bovine skin-derived collagen membranes for the treatment of deep and perforating corneal ulcers in dogs. Twelve cases involving dogs with descemetocele and/or ocular perforation demonstrated advanced corneal stromal damage and compromised eye integrity. Two hundred-micrometer (200 μm) type I bovine skin-derived collagen membranes were inserted into the corneal lesion, which was protected with a 360-degree conjunctival flap, followed by tarsorrhaphy. Evaluations were conducted on days 15, 30, and 60 via a self-designed V-injury score scale. By day 15, patients exhibited significant improvement, with stable intraocular pressure (IOP) and the absence of pain. All patients displayed episcleritis, moderate conjunctival inflammation, and diffuse corneal edema. The score was II in 10 dogs without limbal involvement but with moderate edema. Two dogs had a Grade III injury score with corneal edema exceeding 50% and moderate limbal involvement. By day 30, all patients had remodeled the corneal defect with stable IOPs, but three had moderate edema and superficial vascularization. On day 60, stable IOPs were observed, with four patients developing simple leucoma without involvement with the visual axis or active corneal alteration. The use of 200 μm type I bovine skin-derived collagen membranes, with a 360-degree conjunctival flap and temporary tarsorrhaphy, proves to be an excellent adjuvant in the healing process of deep and perforating corneal lesions in dogs. This combination facilitates corneal remodeling, reducing adverse scarring.</p>","PeriodicalId":23690,"journal":{"name":"Veterinary Research Communications","volume":"49 5","pages":"266"},"PeriodicalIF":2.0,"publicationDate":"2025-07-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144745305","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correction: Epidemiological assessment of bovine brucellosis in Iranian dairy farms: regional Seroprevalence and demographic indicators (2023-2024).","authors":"Alireza Bahonar, Akram Bahreinipour, Maryam Dadar","doi":"10.1007/s11259-025-10841-6","DOIUrl":"https://doi.org/10.1007/s11259-025-10841-6","url":null,"abstract":"","PeriodicalId":23690,"journal":{"name":"Veterinary Research Communications","volume":"49 5","pages":"267"},"PeriodicalIF":2.0,"publicationDate":"2025-07-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144745294","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Absence of hepatitis E virus RNA in bovines from Portugal.","authors":"Sérgio Santos-Silva, Josman D Palmeira, Helena Ferreira, Jesús L Romalde, Helena M R Gonçalves, Wim H M Van der Poel, Maria S J Nascimento, António Rivero-Juarez, João R Mesquita","doi":"10.1007/s11259-025-10839-0","DOIUrl":"10.1007/s11259-025-10839-0","url":null,"abstract":"<p><p>Hepatitis E virus (HEV) is widely recognized as an emerging public health issue in developed countries, with most infections linked to foodborne transmission of genotype HEV-3. This zoonotic genotype can infect a diverse range of mammalian species, including bovine, with pigs serving as the primary reservoir. The aim of the present study was to investigate the occurrence, circulation, and the potential of HEV infection among bovines in Portugal. Stool samples were collected from 166 bovines raised on extensive and intensive farms in Portugal, from June one to July 31, 2015. For the detection of HEV RNA a nested broad-spectrum RT-PCR targeting the ORF1 region was used. HEV RNA was not detected in any of the fecal samples analyzed. Although no HEV RNA was detected in bovine fecal samples, spiking of the samples with mengovirus demonstrated an acceptable RNA recovery rate, ensuring the reliability of RNA extraction and subsequent molecular analysis performed. Further research could provide additional insights into the factors influencing HEV transmission dynamics in bovines and its potential implications for public health.</p>","PeriodicalId":23690,"journal":{"name":"Veterinary Research Communications","volume":"49 5","pages":"265"},"PeriodicalIF":2.0,"publicationDate":"2025-07-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12310789/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144745293","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Immune-antioxidant responses, autophagy, endoplasmic reticulum stress, resistance to Aeromonas sobria in Nile tilapia under arsenic exposure: The mitigating role of a genistein-fortified diet.","authors":"Badriyah S Alotaibi, Amany Abdel-Rahman Mohamed, Yasmina M Abd-Elhakim, Saed A Althobaiti, Shatha B Albattal, Mohamed Mohamed Soliman, Tarek Khamis, Mohamed M M Metwally, Nawal Alsubaie, Rowida E Ibrahim","doi":"10.1007/s11259-025-10837-2","DOIUrl":"https://doi.org/10.1007/s11259-025-10837-2","url":null,"abstract":"<p><p>The immune system of fish is severely impacted by heavy metal pollutants that enter the aquatic system, reducing their survival and resistance to diseases. Arsenic is a widespread heavy metal that contaminates aquatic bodies, impacts fish health, and hinders the sustainability of aquaculture. In the current investigation, we studied the arsenic-hazard consequences on the hematology, serum immunological responses, antioxidant activity, autophagy-related genes, splenic architecture, and protection against Aeromonas sobria in Nile tilapia (Oreochromis niloticus). Additionally, we proposed the ameliorative potential of genistein as a feed additive against arsenic exposure. For this purpose, 160 fish were equally set into four groups, each with four replicates for sixty days. The C (control) and GN (genistein) groups were fed basal diets fortified with 0 and 500 mg genistein/kg diet and were not exposed to arsenic. However, the AS (arsenic) and AS + GN (arsenic + genistein) groups were reared under arsenic conditions (10 µg/L of As₂O₃) and fed on identical diets as the C and GN groups. The outcomes revealed that arsenic exposure induced hematological disorders and anemic conditions in Nile tilapia. The serum immunological and splenic antioxidant enzymes (phagocytic activity, nitric oxide, lysozymes, complement 3, superoxide dismutase, catalase, glutathione peroxidase, glutathione S-transferase, glutathione S-reductase, and reduced glutathione) were substantially lowered, and the malondialdehyde level was increased in the arsenic-exposed fish. Arsenic exposure down-regulated the splenic expression of antioxidant enzymes while upregulated the autophagy-related genes (Beclin-1 and AKT-1) and endoplasmic reticulum (ER) stress-related genes (ATF-6, XBP-1, JNK, and BIP). The arsenic-exposed fish showed the lowest survivability (66.7%) when challenged with A. sobria relative to the control (80%). The dietary intervention of genistein in the Nile tilapia diet mitigated the arsenic-induced hematotoxic, immunotoxic, oxidative stress, autophagy, and ER stress consequences. The histological picture of the spleen and the survivability of the arsenic-exposed fish were restored by feeding on the genistein-fortified diet. Genistein is a promising feed additive for counteracting the harmful effects of arsenic in Nile tilapia, which helps in sustainable aquaculture development.</p>","PeriodicalId":23690,"journal":{"name":"Veterinary Research Communications","volume":"49 5","pages":"268"},"PeriodicalIF":2.0,"publicationDate":"2025-07-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144745295","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of Ferula sp. on sperm cryotolerance, quality, and fatty acid composition in common carp (Cyprinus carpio).","authors":"Abdullatif Ölçülü, Esin Özçiçek, Filiz Kutluyer Kocabaş, Volkan Kızak, Mehmet Kocabaş, Ökkeş Yılmaz","doi":"10.1007/s11259-025-10830-9","DOIUrl":"10.1007/s11259-025-10830-9","url":null,"abstract":"<p><p>Ferula sp. is a perennial herbaceous plant and is used in traditional medicine owing to their antimicrobial, antiviral, aphrodisiac and spermatogenic activities in the world. Hence, Ferula sp. was used in the present study for cryopreservation protocol as a herbal supplement. The impact of different concentrations [0 mM (Control), 30 µg L^-1, 60 µg L^-1, 90 µg L^-1] of Ferula sp. extract on the quality and fatty acid (FAs) composition was assessed in fresh and cryopreserved sperm of Cyprinus carpio. Optimal supplementation of Ferula sp. at a concentration of 60 µg L^-1 in the freezing extender significantly improved post-thawed sperm quality of C. carpio. Sperm motility parameters were assessed using the sperm class analyzer system. The fatty acid (FAs) content in sperm was analyzed using gas chromatography. The results revealed that the increases in motility rate (80.0 ± 3.54%) at 60 µg L^-1 were increased significantly (p < 0.05). Significant differences in sperm parameters [(VCL: curvilinear velocity, VSL: straight line velocity, VAP: average path velocity, and BCF: beat cross frequency)] were observed between the different treatments (p < 0.05). The Monounsaturated fatty acids (MUFAs) showed a slight increase in comparison to fresh spermatozoa during cryopreservation. DHA (Docosahexaenoic acid), LA (Linoleic acid) and ARA (Arachidonic acid) were identified as the major PUFAs (polyunsaturated fatty acid) in the sperm plasma membrane, and however the total PUFAs reduced all treated compared to fresh sperm samples after post-thawing. Overall, the supplementation with Ferula sp. to extender at a concentration of 60 µg L^-1 positively influenced the motility parameters and FAs levels during cryopreservation and, Ferula sp. may act as a promising protective substance for the conservation of valuable fish genetic resources.</p>","PeriodicalId":23690,"journal":{"name":"Veterinary Research Communications","volume":"49 5","pages":"264"},"PeriodicalIF":2.0,"publicationDate":"2025-07-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144733503","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Trehalose prevents glyphosate-induced kidney damage in roosters via inhibiting apoptosis.","authors":"Shuang Liu, Bo Qiu, Yue Li, Hai-Peng Wang, Zhao-Jin Zhong, Lin Wang","doi":"10.1007/s11259-025-10832-7","DOIUrl":"https://doi.org/10.1007/s11259-025-10832-7","url":null,"abstract":"<p><p>Glyphosate (Gly) is a globally prevalent herbicide, which poses significant health risks to human and animals via the food chain. The kidney is the primary excretory organ and a site of residual accumulation during Gly exposure. Trehalose (Tre) has been shown to prevent kidney injury induced by various toxicants due to its anti-apoptotic properties. However, the potential protective effect of Tre on Gly-induced kidney damage in poultry remains unclear. Thus, 60 one-day-old healthy Hy-line Brown roosters were randomly divided into three groups (n = 20), i.e., control group (basal diet without Gly), Gly group (200 mg Gly/kg basal diet), and Gly + Tre group {(200 mg Gly plus 5 g Tre)/kg basal diet}. The experiment lasted for 120 days, and serum and kidney samples were finally collected for respective analysis. Data first showed that Tre treatment can significantly alleviate Gly-induced renal injury in roosters, as evidenced by serum levels of creatinine and blood urea nitrogen as well as renal histopathological observations. Tre also significantly inhibited the elevated abundance of renal injury marker proteins KIM-1 and NGAL induced by Gly. Meanwhile, Tre obviously alleviated Gly-induced ultrastructural abnormalities in the nuclei of renal tissues. Additionally, Tre markedly inhibited Gly-induced apoptotic assays in rooster kidneys, as demonstrated by the results from TUNEL staining and the protein abundance of apoptotic markers cleaved caspase-3 and cleaved PARP. In summary, these findings suggest that Tre exerts its protective effects against Gly-induced kidney damage in roosters via inhibiting apoptosis.</p>","PeriodicalId":23690,"journal":{"name":"Veterinary Research Communications","volume":"49 5","pages":"263"},"PeriodicalIF":1.8,"publicationDate":"2025-07-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144709060","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative evaluation of fresh and lyophilized Nile tilapia fish skin for enhancing wound healing in a donkey model.","authors":"Kamal H Hussein, Mahmoud Soliman, Mahmoud Abd-Elkareem, Ahmed Abdelrahiem Sadek","doi":"10.1007/s11259-025-10821-w","DOIUrl":"10.1007/s11259-025-10821-w","url":null,"abstract":"<p><p>Recent advancements in wound care have explored the use of biological dressings, including fish skin, due to its rich collagen content and bioactive components that promote healing. This study aimed to evaluate the effectiveness of fresh and lyophilized Nile tilapia skin in enhancing full thickness skin wound healing in donkeys. Nile tilapia skin was collected, thoroughly washed, lyophilized, and sterilized. Five female donkeys were used in the study, each receiving three full-thickness skin wounds (2 cm x 2 cm) on each side of the back after aseptic preparation and local anesthesia, resulting in a total of six wounds per animal. The wounds were assigned to three groups: control (treated with saline), fresh fish skin, and lyophilized fish skin. Macroscopic wound assessment was performed and skin samples were collected on days 14 and 28 for histological examination using hematoxylin and eosin (H&E) and Crossman's trichrome staining. Results revealed that treatment with lyophilized fish skin significantly accelerated wound contraction and epithelialization compared to the control and fresh fish skin-treated groups. On day 14, wound contraction rates were 43.57% ± 0.87 for lyophilized fish skin, 41.32% ± 0.26 for fresh fish skin, and 32.48% ± 0.39 for the control. By day 28, contraction rates increased to 74.37% ± 0.77, 66.92% ± 0.31, and 56.88% ± 0.73, respectively. Histological analysis showed enhanced collagen deposition and angiogenesis in the lyophilized fish skin group. In conclusion, lyophilized Nile tilapia skin is a promising and cost-effective biomaterial for enhancing wound healing, offering a practical solution for field veterinarians in low-resource settings.</p>","PeriodicalId":23690,"journal":{"name":"Veterinary Research Communications","volume":"49 5","pages":"262"},"PeriodicalIF":1.8,"publicationDate":"2025-07-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12287218/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144691738","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}