Enterococcus species identified by MALDI-TOF MS in milk from dairy cow mastitis cases and herd surveys.

IF 2 3区农林科学 Q2 VETERINARY SCIENCES

Veterinary Research Communications Pub Date : 2025-07-23 DOI:10.1007/s11259-025-10834-5

Fernando Ulloa, Martina Penati, Valentina Monistero, Valerio Bronzo, Paolo Moroni, Miguel Salgado, Maria Filippa Addis

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引用次数: 0

Abstract

Enterococcus species are increasingly recognized as mastitis pathogens in dairy cows. Reliable species information is required for correctly defining the regional epidemiology of enterococcal mastitis, establishing its relationships with management variables, and understanding its impact on udder health. We investigated the species distribution of enterococci in bovine milk from subclinical mastitis (SCM) and clinical mastitis (CM) cases and full herd surveys (HS) using MALDI-TOF MS as identification method. A total of 21,864 milk samples from 106 dairy herds were routinely collected and analyzed according to the National Mastitis Council (NMC) guidelines over one year. Enterococcus spp. were found in 4.86% of CM, 5.05% of SCM, and 1.37% of HS milk samples. Overall, E. saccharolyticus was the most prevalent species (55.65%), followed by E. faecium (27.25%), and E. cecorum (5.51%), which showed a significant association with SCM (p-value = 0.0128). This study provides novel data on the Enterococcus species distribution in full herds survey and mastitis cases, highlighting the relevance of MALDI-TOF MS in refining enterococcal mastitis epidemiology, as true prevalence and species distribution may have been skewed by conventional biochemical tests.

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MALDI-TOF质谱法在奶牛乳腺炎病例和牛群调查中鉴定出肠球菌种类。

肠球菌越来越被认为是奶牛乳腺炎的病原体。要正确定义肠球菌性乳腺炎的区域流行病学，建立其与管理变量的关系，并了解其对乳房健康的影响，需要可靠的物种信息。采用MALDI-TOF质谱法对亚临床乳腺炎（SCM）和临床乳腺炎（CM）病例和全群调查（HS）的牛乳中肠球菌的种类分布进行了研究。根据国家乳腺炎委员会（NMC）的指导方针，在一年的时间里，从106个奶牛群中常规收集和分析了21,864份牛奶样本。牛奶样品中检出肠球菌4.86%，牛奶样品中检出肠球菌5.05%，牛奶样品中检出肠球菌1.37%。总体而言，溶糖E.（55.65%）、屎E.（27.25%）、盲肠E.（5.51%）与SCM呈显著相关（p值= 0.0128）。该研究提供了全群调查和乳腺炎病例中肠球菌物种分布的新数据，强调了MALDI-TOF MS在改善肠球菌乳腺炎流行病学方面的相关性，因为传统的生化测试可能会扭曲真实的流行率和物种分布。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Veterinary Research Communications 农林科学-兽医学

CiteScore

2.50

自引率

0.00%

发文量

173

审稿时长

3 months

期刊介绍： Veterinary Research Communications publishes fully refereed research articles and topical reviews on all aspects of the veterinary sciences. Interdisciplinary articles are particularly encouraged, as are well argued reviews, even if they are somewhat controversial. The journal is an appropriate medium in which to publish new methods, newly described diseases and new pathological findings, as these are applied to animals. The material should be of international rather than local interest. As it deliberately seeks a wide coverage, Veterinary Research Communications provides its readers with a means of keeping abreast of current developments in the entire field of veterinary science.