Veterinary Research Communications最新文献

{"title":"Zoonotic relevance of multidrug-resistant bacteria in parrots with respiratory illness.","authors":"Ahmed Samir, Tarek Mosallam, Hassan Aboul-Ella, Aisha Ali, Ojena Samir, Mohamed Hegab, Mark Erian, Fady Youssef, Hala Zaher","doi":"10.1007/s11259-025-10752-6","DOIUrl":"https://doi.org/10.1007/s11259-025-10752-6","url":null,"abstract":"<p><p>Nowadays, research attention is paid to the investigation of bacterial pathogens in the cloaca of parrots rather than the nasal niche, which is largely ignored. Therefore, this study aimed to investigate the nasal carriage of multidrug-resistant bacteria with zoonotic potential in parrots suffering from respiratory illness. Nasal swabs were collected from 75 sick parrots with respiratory illness, and they were subjected to microbiological isolation and identification, followed by antimicrobial susceptibility testing. Escherichia coli, Klebsiella pneumoniae, Proteus mirabilis, and Staphylococcus aureus were isolated with a prevalence rate of 36%, 32%, 26.7%, and 9.3%, respectively, while one isolate (1.3%) of Staphylococcus pseudointermedius, Staphylococcus simulans, Staphylococcus sciuri, and Enterococcus faecalis was identified. E. coli, K. pneumoniae, and P. mirabilis were investigated for ESBL genes, Staphylococcus species for the mecA gene, followed by SCCmec typing, and E. faecalis for the vanA and vanB genes. Regarding beta-lactamase-encoding genes, bla_TEM (97.6%), bla_SHV (48.8%), and bla_CTX-M (39%) gene families were detected, while bla_OXA was not found. Sequencing of bla_CTX-M in one strain of E. coli, K. pneumoniae, and P. mirabilis revealed bla_CTX-M-15. The mecA was determined in three S. aureus and one S. sciuri strain, and the SCCmec typing of three MRSA isolates yielded type V, whereas type I in S. sciuri. Only the vanA gene was recognized in the E. faecalis strain. Moreover, 67.1% of bacterial isolates exhibited multidrug resistance. These findings highlight the potential role of parrots in the transmission of multidrug-resistant zoonotic bacteria, which may pose a threat to human contacts.</p>","PeriodicalId":23690,"journal":{"name":"Veterinary Research Communications","volume":"49 4","pages":"194"},"PeriodicalIF":1.8,"publicationDate":"2025-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12062053/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144001361","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Approaching equine infectious anemia in regions dominated by grade-working animals - a case of Pará, a state in the Brazilian Amazon.","authors":"Ana Paula Vilhena Beckman Pinho, Fernando Ferreira, Jeferson Jacó Fuck, Jefferson Pinto de Oliveira, Ricardo Augusto Dias, José Henrique Hidebrand Grisi-Filho, Marcos Bryan Heinemann, Evelise Oliveira Telles, Marcos Amaku, José Soares Ferreira Neto","doi":"10.1007/s11259-025-10759-z","DOIUrl":"https://doi.org/10.1007/s11259-025-10759-z","url":null,"abstract":"<p><p>Considering that control strategies for Equine Infectious Anemia, based on the serological diagnosis of equids and the removal of positive animals, may not be optimal for developing countries with an absolute predominance of grade working animals, this study aimed to address this issue based on the epidemiological situation of the disease in Pará, a state in the Brazilian Amazon. Pará was divided into five regions, and within each region a pre-established number of farms were randomly selected. Within each farm, a pre-set number of animals were randomly selected and submitted to Agar Gel Immunodiffusion test. A questionnaire was administered on the farms to identify risk factors for the disease. In total, 2,718 equids (horses, mules and donkeys) from 654 farms were tested. The prevalence at farms and animal level in the state was 34.5% (95% CI: 30.8-38.4) and 15.8% (95% CI: 14.4-17.4), with significant regional differences. Equids from farms with 11 or more equids (OR = 2.32 [95% CI: 1.38-3.92]) and those that shared water sources with other farms (OR = 1.76 [95% CI: 1.26-2.46]) were more likely to be infected. The average sensitivity of the surveillance system for detecting infected farms ranged from 0.92 to 1.14%, which is insufficient to disrupt the endemic balance of the disease in the state, demanding a reassessment. The key elements of this process, as well as the potential strategies to be implemented, have been discussed in the context of Pará and can be extended to regions with similar characteristics.</p>","PeriodicalId":23690,"journal":{"name":"Veterinary Research Communications","volume":"49 4","pages":"192"},"PeriodicalIF":1.8,"publicationDate":"2025-05-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143988739","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Survival and viability of Toxoplasma gondii oocysts under natural dry season conditions in the Brazilian semi-arid region.","authors":"Ana Luzia Peixoto Silva, Brendo Andrade Lima, Victor Hugo Alves Sousa Formiga, Estefany Ferreira Lima, Geraldo Moreira Silva Filho, Welitânia Inácia Silva, Jordania Oliveira Silva, Felipe Boniedj Ventura Alvares, Vinícius Longo Ribeiro Vilela, Thais Ferreira Feitosa","doi":"10.1007/s11259-025-10757-1","DOIUrl":"https://doi.org/10.1007/s11259-025-10757-1","url":null,"abstract":"<p><p>Due to the outbreaks of toxoplasmosis in Brazil, primarily caused by oocysts, the objective of this study was to evaluate how Toxoplasma gondii oocysts behave in the Brazilian semi-arid region and the influence of environmental conditions on the occurrence of outbreaks in the region. Cats were infected with mouse brains containing T. gondii cysts and subjected to five different environmental conditions: Group 1: Positive fecal samples exposed to sunlight and covered with 2 cm of common sand; Group 2: Positive fecal samples exposed to sunlight on the surface of the sand; Group 3: Positive fecal samples in the shade, covered with 2 cm of common sand; Group 4: Positive fecal samples in the shade on the surface of the sand; Group 5: Positive fecal samples kept under ideal temperature and humidity conditions in Biochemical Oxygen Demand (BOD) - Control. Samples were collected initially after 12 h of environmental exposure and subsequently every 12 h to evaluate the presence of oocysts and the sporulation rate. Temperature and humidity were monitored throughout the experiment. After 24 h of exposure, the oocysts were completely destroyed, and 92% of the oocysts were sporulated within 12 h of initial exposure. Soil temperatures reached up to 57 °C, with humidity levels as low as 15%. The study concludes that high temperatures and low humidity are decisive factors in the destruction of T. gondii oocysts, which are rapidly inactivated when exposed to the semi-arid climate of Northeastern Brazil during the dry season.</p>","PeriodicalId":23690,"journal":{"name":"Veterinary Research Communications","volume":"49 4","pages":"191"},"PeriodicalIF":1.8,"publicationDate":"2025-05-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143988742","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Histopathological and immunohistochemical characteristics of chordae tendineae affected by degenerative processes in canine myxomatous mitral valve disease.","authors":"Justyn Gach, Agnieszka Mackiewicz, Izabela Janus-Ziółkowska, Agnieszka Noszczyk-Nowak","doi":"10.1007/s11259-025-10761-5","DOIUrl":"https://doi.org/10.1007/s11259-025-10761-5","url":null,"abstract":"<p><p>Myxomatous mitral valve disease is a major problem in canine cardiology. Degenerative changes extend from the valve leaflets to reach the rest of the subvalvular apparatus. The chordae tendineae (CT) play a key role in the mechanics of the mitral valve and ensure unidirectional blood flow through the heart. Degenerative changes within the chordae tendineae can severely disrupt their function, ultimately leading to an episode of chordae rupture. The study aimed to analyse the structure of healthy and degenerated CTs via histopathology and immunohistochemistry. The mitral valve was assessed macroscopically using the Whitney scale to identify degenerative changes. The chordae tendineae were classified on a four-grade scale (0-3) on the basis of structural changes and subsequently analysed through immunohistochemical staining with antibodies targeting collagens I, III, and IV, as well as fibronectin, chondroitin, and tenascin. The findings revealed alterations in the extracellular matrix in degenerated chordae tendineae.</p>","PeriodicalId":23690,"journal":{"name":"Veterinary Research Communications","volume":"49 4","pages":"190"},"PeriodicalIF":1.8,"publicationDate":"2025-05-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12055879/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144052664","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Clinical, pathological, and molecular aspects of malignant catarrhal fever in Russia: a case report.","authors":"Pavel A Andriyanov, Olga I Zakharova, Pavel A Zhurilov, Anastasia I Tutrina, Daria D Kashina, Olga A Burova, Elena A Liskova, Ekaterina A Shirokova, Ivan V Yashin, Andrey A Blokhin","doi":"10.1007/s11259-025-10758-0","DOIUrl":"https://doi.org/10.1007/s11259-025-10758-0","url":null,"abstract":"<p><p>Malignant catarrhal fever (MCF) is a highly fatal viral disease caused by ovine gammaherpesvirus-2 (OvHV-2) that primarily affects cattle around the world. Sheep act as asymptomatic carriers, silently perpetuating the virus. Despite its global presence, MCF remains poorly understood in Russia due to limited surveillance, voluntary disease reporting, and its exclusion from routine differential diagnoses. This study aimed to investigate a suspected case of MCF in a Yakut-breed cow on a private farm in Pskov Oblast, Russia, bordering Estonia. The cow resided in a mixed sheep and cattle herd with direct sheep contact. Clinical examination and histological analysis revealed characteristic MCF lesions. Phylogenetic analysis of the cow's OvHV-2 revealed a mosaic structure. The polymerase01, gene clustered with strains from the Netherlands and Germany, while the tegument gene grouped with isolates from India and Egypt. This highlights the global nature of OvHV-2 genetic diversity. Overall, this study provides the first documented case of MCF in Russia and underscores the sporadic nature of the disease in cattle. The identification of novel pathological changes and the unique viral genetic makeup necessitate further investigation of MCF in Russia.</p>","PeriodicalId":23690,"journal":{"name":"Veterinary Research Communications","volume":"49 4","pages":"189"},"PeriodicalIF":1.8,"publicationDate":"2025-05-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144017110","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"DNA porcine viruses detected on fresh liver samples destined for human consumption.","authors":"Mariana Antunes Rezende, Yasmin Ampese Maté, Jean Francisco Maldaner Lui, Ana Clara Seibel, Rafael Frandoloso, Luiz Carlos Kreutz","doi":"10.1007/s11259-025-10753-5","DOIUrl":"https://doi.org/10.1007/s11259-025-10753-5","url":null,"abstract":"<p><p>Pork meat and pork-derived products are major sources of protein, but they might be important vehicles for transmitting viruses to humans. Here we aimed to evaluate whether pig enzootic viruses could be found in liver samples destined for foodstuff manufacture. We collected 140 liver samples in a slaughterhouse for DNA extraction and viral detection by real-time or conventional PCR. The samples were from 14 swine herds (n = 10/herd) located in Rio Grande do Sul state, Brazil. We found 93 (66.4%) samples positive for swine torque teno virus (TTSuV); out of these, 29 samples (31.2%) were positive for TTSuV1, 39 (41.9%) for TTSuV2 and 25 (26.9%) for both TTSuV genotypes. Porcine circovirus type 2 (PCV2) DNA was found on 24 (17.1%) samples, out of which 23 were PCV2d and only one was PCV2b. Half of the samples from each farm were tested for adenoviruses (AdV) and 44 (62.8%) were positive: by analyzing the melting curve, the samples were classified as adenoviruses of human (HAdV), canine (CAdV), and bird (AVAdV) origin in 39 (55.7%), 11 (15.7%) and 3 (4.2%) of the samples. Porcine parvovirus (PPV) DNA was detected in 5 (7.1%) of the 70 samples analyzed. Pork liver is commonly used for making pate, sausages, and mortadella which, in this situation, could harbor these viruses. Further processing of foodstuff is likely to inactive viral agents; however, our data indicates that pork-derived meat might contain viruses that eventually could reach the food chain and be harmful to immunocompromised individuals.</p>","PeriodicalId":23690,"journal":{"name":"Veterinary Research Communications","volume":"49 4","pages":"188"},"PeriodicalIF":1.8,"publicationDate":"2025-05-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144050642","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mycological and molecular identification of mycoses involved in mastitis from Holstein dairy cattle with special reference to Candida albicans.","authors":"Hayat Fayed, Asmaa A Elgendy, Salma A Shoulah, Samar M Moustafa, Ahmed Maher, Ahmed F Hikal, Ahmed Abdeen, Ekramy Elmorsy, Mohamed E Mohamed, Helal F Hetta, Heba Allah Elbaghdady, Afrina Mustari, Samah F Ibrahim, Ateya M Ibrahim, Marawan A Marawan","doi":"10.1007/s11259-025-10744-6","DOIUrl":"https://doi.org/10.1007/s11259-025-10744-6","url":null,"abstract":"<p><p>Globally, mastitis is an incredibly devastating, multifactorial disease that affects the dairy industry. However, cases of mycotic mastitis in dairy cows have increased in recent years. This study aimed to investigate the prevalence of mycotic mastitis with special reference to Candida albicans in Holstein-Friesian dairy cows from some farms in Menoufia province, Egypt. For the study, 150 milk samples (n = 150, 20 from healthy cows and 130 from cows with subclinical and clinical mastitis) were collected from 15 smallholder dairy farms located in three localities (Quesina, Elbagour, and Shibin El-Kom) five farms from each. The samples were subjected to mycological culture and subsequently verified using traditional morphological and biochemical tests to confirm fungal characteristics followed by antimycotic susceptibility testing using Vitec 2 system. Finally, molecular identification was carried out via PCR and phylogenetic analysis. 27 samples out of 130 were positive for fungal culture representing (20.77%). Candida species were recorded the highest percentage (62.96%) of the detected fungi (17/27). In addition, C. albicans was the predominant species (3/27), which corresponds to 11.11%. Antifungal susceptibility testing of the fungal isolates showed the highest susceptibility to micafungin, followed by caspofungin (88.88% and 86.19%, respectively). However, the lowest susceptibility was noted against amphotericin B (50.55%). The three isolates of C. albicans were confirmed by PCR by amplifying the ITS region, which was then sequenced for phylogenetic analysis. The sequences have been deposited in GenBank under the following accession numbers: OR97266, OR97267, and OR97268. Our results indicate that Candida spp., especially C. albicans, is one of the major causes of mycotic mastitis in dairy cows in Egypt. Moreover, micafungin might be the efficient medication for treating these cases of mycotic mastitis, followed by caspofungin.</p>","PeriodicalId":23690,"journal":{"name":"Veterinary Research Communications","volume":"49 4","pages":"187"},"PeriodicalIF":1.8,"publicationDate":"2025-05-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144064889","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of glutamine supplementation on the growth performance, antioxidant capacity, immunity and intestinal morphology of cold-stressed prestarter broiler chicks.","authors":"Juanjuan Wu, Wenxin Qiu, Guiyao Li, Haoneng Guo, Sifa Dai, Guanhong Li","doi":"10.1007/s11259-025-10756-2","DOIUrl":"https://doi.org/10.1007/s11259-025-10756-2","url":null,"abstract":"<p><strong>Background: </strong>Cold stress has important effects on the growth and production of broiler chickens. Glutamine (Gln) is a conditionally essential amino acid that plays an important role in promoting intestinal development and enhancing immune function. The aim of this study was to investigate the effects of Gln supplementation on the growth performance and health of cold-stressed prestarter broiler chicks.</p><p><strong>Methods and results: </strong>A total of 375 1-day-old male SZ901 broilers were randomly divided into five groups (CON, CS, GLN1, GLN2, GLN3). Birds in the CON and CS groups were provided with normal drinking water, while the GLN1, GLN2 and GLN3 groups were provided with water supplemented with 0.4%, 0.8% and 1.2% Gln, respectively. At d7, birds in groups CS, GLN1, GLN2, and GLN3 were stressed at 12 ± 1 ℃ for 12 h. The results showed that cold stress significantly decreased the growth performance, serum antioxidant capacity and antibody concentrations, small intestine villus structure, and increased the gene expression of intestinal inflammatory factors of broiler chicks compared with the CON group (P < 0.05). Compared with the CS group, Gln supplementation exhibited increased growth performance, serum antioxidant capacity and antibody concentrations, gene expression levels of intestinal tight junction protein, villus height and villus height to crypt depth ratio (V/C) of small intestine, and decreased mRNA expression level of intestinal inflammatory factors (P < 0.05).</p><p><strong>Conclusions: </strong>Gln supplementation ameliorated the impact of cold stress to a large extent as it promoted the development of the intestine and immune system and enhanced the antioxidant enzyme system in cold-stressed prestarter chicks.</p>","PeriodicalId":23690,"journal":{"name":"Veterinary Research Communications","volume":"49 3","pages":"183"},"PeriodicalIF":1.8,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144041172","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Phylogenetic and sequence analysis of the co-circulating lineages in the canine distemper virus complexity from Northern Vietnam (2017-2023).","authors":"Huong Thi Thanh Doan, Khue Thi Nguyen, Roan Thi Do, Luu Minh Duc, Pham Van Tiem, Thanh Hoa Le","doi":"10.1007/s11259-025-10750-8","DOIUrl":"https://doi.org/10.1007/s11259-025-10750-8","url":null,"abstract":"<p><p>Morbillivirus canis, commonly known as Canine distemper virus (CDV), causes Canine distemper (CD) in carnivore species across the world and spreads via cross-species transmission between domestic and wildlife canids, non-canid carnivores, and omnivores. We report on the Asia-4 lineage of CDV previously documented in Thailand and China, which has emerged for the first time in northern Vietnam since 2017. The phylogenetic analysis of 121 complete nucleotide CDS (coding sequence) of the hemagglutinin (H) gene, including 20 from this study, and 101 reference strains from all 19 lineages of CDVs revealed that the Asia-4 lineage co-circulated with the Asia-1 in Vietnam. The evolutionary distances (ED) or genetic distances between the Vietnamese and typical Asia-4 strains was low, substantially distinguishing them from other lineages. The immunodominant epitopes, host-switching, and glycosylation sites in Asia-4 viruses have several site alterations, providing fair insights into the effectiveness of the America-1-based vaccine. In addition to Thailand, China, Russia, and Mongolia, the Asia-4 lineage has appeared in northern Vietnam. Whether via canine and pet or wild animal trade/border crossing trafficking to Vietnam, remains to be studied. Our findings contribute to a better understanding of CDV molecular epidemiology and the suitable application of lineage-antigenic matching vaccines, as well as the complicated co-circulation of multiple-lineage strains in Vietnam.</p>","PeriodicalId":23690,"journal":{"name":"Veterinary Research Communications","volume":"49 3","pages":"186"},"PeriodicalIF":1.8,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144014353","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impact of autologous platelet-rich plasma used for treatment of oligozoospermia in dogs on the quality of semen and testicular blood flow.","authors":"Alaa Mohamed, Mohamed Fathi, Ashraf A Shamaa, K H El Shahat","doi":"10.1007/s11259-025-10734-8","DOIUrl":"https://doi.org/10.1007/s11259-025-10734-8","url":null,"abstract":"<p><p>Platelet-rich plasma (PRP) is widely used in regenerative medicine, and the current study aimed to investigate the effects of autologous PRP on semen characteristics, testicular blood flow, and testosterone levels in the treatment of oligozoospermia in dogs. Ten stray male dogs diagnosed with oligozoospermia were included in the study. The dogs were randomly assigned to two groups: Group I, the control group (n = 5), which received no treatment, and Group II, the PRP group (n = 5), which received a single intra-testicular injection of 0.5 mL of autologous PRP into each testicle. Testicular hemodynamics, hormonal and biochemical analysis and semen parameters were assessed for both groups and the examination was extended to 8 weeks. The results demonstrated that dogs treated with PRP showed significantly higher values of End Diastolic Velocity (EDV) and Peak Systolic Velocity (PSV) (P < 0.001) at the 6^th to 8^th weeks post-injection, compared to baseline (day 0) values. Conversely, the values of Resistance Index (RI) and Pulsatility Index (PI) showed a significant decrease (P < 0.001) in group II from week 2 to week 8. Additionally, PRP treatment led to significant increases in sperm concentration, motility percentage, and the proportion of live and normal spermatozoa (P < 0.001), with maximum values observed at 28 and 60 days post-treatment, compared to day 0. Moreover, serum testosterone and nitric oxide (NO) levels were significantly higher (P < 0.001) in group II and remained elevated through the 8^th week following injection, compared to baseline values and group I. In conclusion, this study demonstrates that autologous PRP treatment effectively increases sperm concentration, motility, and normal spermatozoa, improves testicular blood flow, and elevates testosterone and NO levels. These findings suggest that PRP may be a promising therapeutic option for the treatment of oligozoospermia in dogs. Further studies are needed to confirm and expand upon these results.</p>","PeriodicalId":23690,"journal":{"name":"Veterinary Research Communications","volume":"49 3","pages":"182"},"PeriodicalIF":1.8,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12045825/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143998319","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}