Veterinary Research Communications最新文献

{"title":"Theileria parva genetics, prevalence and vaccination practices in Zimbabwe and the African region and the prospects for vaccine development: a systematic review.","authors":"Daniel Mukandabvute, Noah Herbert Paul, Fanuel Songwe, Maud Chipatiko, Liana-Lisa Sakwa, Nyasha Chin'ombe, Leonard Madzingaidzo","doi":"10.1007/s11259-025-10715-x","DOIUrl":"https://doi.org/10.1007/s11259-025-10715-x","url":null,"abstract":"<p><strong>Introduction: </strong>January disease causes the deaths of over 55,000 cattle valued at approximately US$ 17 million annually in Zimbabwe. The locally developed Boleni stabilate vaccine is in use for controlling the disease. In the present review, we show the current knowledge of the genetic variation and population structure of Theileria parva parasite and its implications on the epidemiology and control of the parasite in eastern and southern Africa, with a major emphasis on Zimbabwe.</p><p><strong>Methods: </strong>A systematic review was conducted to analyse the reports available in literature in order to map the Theileria parva genetic diversity profile. A total of 103 studies met the criteria for analysis and were included in the review.</p><p><strong>Results: </strong>The reports retrieved in this study show that East Coast fever and Corridor disease have been extensively sequenced to establish the parasite population genetic structure. One report described genetic diversity in January disease, with no sequencing tools included. The live sporozoite stabilate administered as infection without concurrent treatment remains the vaccination approach of choice in January disease.</p><p><strong>Discussion: </strong>The adoption of population genetics led to the determination of genotypes that were employed in the development of the Muguga cocktail vaccine. To date, vaccination against East Coast fever is reported to reduce mortality rate. The Boleni isolate remains in use for vaccination against uncharacterized populations of January disease in spite of increased mortality rates extensively reported from 2015 to date. There is need to adopt comparative genomics in vaccine development for a more effective vaccine.</p>","PeriodicalId":23690,"journal":{"name":"Veterinary Research Communications","volume":"49 3","pages":"146"},"PeriodicalIF":1.8,"publicationDate":"2025-03-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143664641","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of dietary phytase and sex on behavioral responses in castrated male and female piglets.","authors":"Gustavo Zigovski, Elmer Modesto Elvir Hernandez, Isabela Cristina Colaço Bez, Gabriel Kioshi Cavalari Nakamura, Mariana Regina Rosa Catoia, Ana Julia Carrasco Buzatto, Ruan Rolnei Daros, Paulo Levi de Oliveira Carvalho, Leandro Batista Costa","doi":"10.1007/s11259-025-10714-y","DOIUrl":"https://doi.org/10.1007/s11259-025-10714-y","url":null,"abstract":"<p><p>This study investigated whether adding phytase to the diet of castrated male and female piglets modulates behavior and if the response differs between sexes. The experiment involved 126 piglets, weaned at 21 days of age, with an average weight of 6.53 ± 1.29 kg. Six treatments were employed, with seven replications and three animals per pen. The treatments were PC: positive control diet - adequate nutritional levels and without phytase; NC: negative control diet - reduced levels of nutrients and metabolizable energy, and without phytase; negative control diets with 500; 1.000; 1.500 and 2.000 FTU/kg of feed. Piglets were subjected to open field (OF), novel object (NO) and reactivity during weighing (RDW) behavioral tests. In the OF, females showed longer exploration time (p = 0.036) and explored a greater number of quadrants (p = 0.031). Conversely, males remained more static (p < 0.01) and vocalized more (p = 0.041). For the NO, females exhibited shorter latency to reach the novel object (p = 0.040). In the RDW, male piglets exhibited greater movement resistance (p = 0.011) and vocalized more frequently (p = 0.038). The addition of phytase did not affect piglet behavior; however, the results suggest that females are more explorative, while castrated males show increased immobility in response to fear.</p>","PeriodicalId":23690,"journal":{"name":"Veterinary Research Communications","volume":"49 3","pages":"139"},"PeriodicalIF":1.8,"publicationDate":"2025-03-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143626046","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of intramammary infusion of Minthostachys verticillata essential oil in cows at drying-off on microbiological and immunological parameters, and milk quality during the subsequent lactation.","authors":"Sofía Arsaute, Elina B Reinoso, María E Cecchini, Ivana D Montironi, Dardo A Roma, Fabiana D Éramo, Marcela Moressi, Lorena Decara, Javier Macchiavelli, Andrea Ariño, Laura Noelia Cariddi","doi":"10.1007/s11259-025-10709-9","DOIUrl":"https://doi.org/10.1007/s11259-025-10709-9","url":null,"abstract":"<p><p>This study evaluated the effects of EO administered via intramammary inoculation in dairy cows during the drying-off period and its impact on milk quality in the subsequent lactation. Ten pregnant Holstein cows, free of mastitis, were selected from an organic dairy farm in Córdoba, Argentina. Twenty-four quarters were inoculated with EO: eight received 0.25 g, eight received 0.50 g, and eight received 1.00 g. Control groups included uninoculated quarters and quarters treated with cephapirin. Milk samples were collected before treatment and within 4 days after calving to assess microbial count and somatic cell counts (SCC). The expression of TNF-α, IL-6, and IL-10 was quantified using qPCR and malondialdehyde (MDA) levels were also measured. Blood samples were collected before treatment and within 4 days after calving to determine serum IL-6 and IL-4 levels using ELISA. No significant effects were observed on milk's physical-chemical properties following EO or cephapirin treatment. EO at 1.00 g significantly reduced microbial load (p < 0.05), comparable to cephapirin. SCC increased with all EO doses (p < 0.01, p < 0.05, p < 0.05, respectively). Differential cell counts showed an increase in macrophages after 1.00 g EO treatment. The relative expression of TNF-α, IL-6, and IL-10 decreased without statistical significance. MDA levels significantly increased with cephapirin (p < 0.001), but EO had no effect. Serum IL-6 and IL-4 levels remained unchanged. In conclusion, intramammary EO inoculation enhanced immune response, reduced microbial load, and did not induce lipid peroxidation, suggesting its potential as a preventive treatment for mastitis.</p>","PeriodicalId":23690,"journal":{"name":"Veterinary Research Communications","volume":"49 3","pages":"140"},"PeriodicalIF":1.8,"publicationDate":"2025-03-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143626238","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Assessment of the endectocide efficacy of a pour-on topical solution containing fluazuron, fipronil and eprinomectin in cattle.","authors":"Daniel Pacheco de Melo, Carolina Buzzulini, Gustavo Felippelli, Lucas Vinícius Costa Gomes, Laís Aparecida Torres, Pedro Vale Moreira, Gabriele Zaine Teixeira Debortoli, Thaís Rabelo Dos Santos-Doni, Alvimar José da Costa","doi":"10.1007/s11259-025-10710-2","DOIUrl":"https://doi.org/10.1007/s11259-025-10710-2","url":null,"abstract":"<p><p>The present study aimed to evaluate the effectiveness of a new formulation composed of the chemical active ingredients benzoylphenylurea, phenylpyrazole and macrocyclic lactone, which have different mechanisms of action on the most important parasites, such as R. microplus, H. irritans, D. hominis larvae, C. hominivorax larvae and nematodes. The six experiments were conducted in Formiga (Bananal and Bela Vista farms) in the Center-West region of Minas Gerais, Brazil. This study introduces a new topical formulation containing 3.0% Fluazuron, 1.25% Fipronil, and 0.5% Eprinomectin, which has demonstrated high efficacy against R. microplus in both natural (97%) and experimental (95%) infestations. Additionally, the formulation was effective as a larvicide against D. hominis (98%) and C. hominivorax (100%), as well as against H. irritans (98%) and as an anthelmintic (97%). Its broad-spectrum activity and prolonged efficacy make it a tool for managing parasitic infestations in cattle. However, the potential for resistance development and the need for strategic application highlight the importance of integrated control programs that combine chemical and nonchemical approaches to ensure sustainable parasite management.</p>","PeriodicalId":23690,"journal":{"name":"Veterinary Research Communications","volume":"49 3","pages":"138"},"PeriodicalIF":1.8,"publicationDate":"2025-03-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143617326","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Typing of hemotropic Mycoplasma in Egyptian cats: first detection and phylogenetic analysis of Candidatus Mycoplasma turicensis.","authors":"Mahmoud S Safwat, Noha M Bakry, Amany D Bahr, Ahmed Orabi, Asmaa A Rayan, Ghada M Khalil, Salma W Abdelhaleem, Omnia H Refaei","doi":"10.1007/s11259-025-10693-0","DOIUrl":"10.1007/s11259-025-10693-0","url":null,"abstract":"<p><p>Mycoplasma haemofelis (Mhf), Candidatus Mycoplasma haemominutum (CMhm), and Candidatus Mycoplasma turicensis (CMt) are the major feline hemotropic Mycoplasma (FHM) species identified in cats worldwide. Data concerning FHM in Egypt is limited; therefore, the current study aimed to gain further insights into disease epidemiology by investigating FHM molecular prevalence, risk factors, and hemogram abnormalities in 246 Egyptian cats (shelter-housed and client-owned) during 2022-2024. Additionally, 16 S rRNA gene-based maximum-likelihood (ML) phylogenetic analysis was performed for all CMt-positive samples for the first time in Egypt. FHM was detected in 16.3% (n = 40) of cats and typed as CMhm, Mhf, and CMt in 15.4% (n = 38), 3.6% (n = 9), and 2.4% (n = 6) of cats, respectively. Single CMhm and Mhf infections were detected in 12.6% (n = 31) and 0.8% (n = 2) of cats, respectively. Dual (Mhf and CMhm) and triple (Mhf, CMhm, and CMt) infections were found in 0.4% (n = 1) and 2.4% (n = 6) of cats, respectively. CMhm was significantly associated with single infections rather than coinfections compared to other FHM species. Male sex and anemia were identified as predictors of FHM PCR positivity. FHM-infected cats had significantly lower hematocrit %, RBC counts, and hemoglobin concentrations than non-infected ones. Egyptian CMt sequences showed 97.6-100% nucleotide identity with each other. Egyptian and reference CMt strains represented nine nucleotide sequence types clustered into three well-supported clades on the ML tree without clear geographic distinction. The data generated in this study, conducted in Egypt, is crucial for enhancing our understanding of disease epidemiology and implementing effective preventive measures.</p>","PeriodicalId":23690,"journal":{"name":"Veterinary Research Communications","volume":"49 3","pages":"136"},"PeriodicalIF":1.8,"publicationDate":"2025-03-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11897094/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143606450","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A retrospective study of injectable versus inhalation anesthesia for umbilical surgery in Japanese black calves.","authors":"Shogo Sato, Chihiro Kanno, Riku Ueda, Yosuke Maeda, Fumiaki Takahashi","doi":"10.1007/s11259-025-10711-1","DOIUrl":"https://doi.org/10.1007/s11259-025-10711-1","url":null,"abstract":"<p><p>Umbilical diseases are associated with a decreased market value and increased mortality in calves, and laparotomy is often performed in the field. This retrospective study compared the effects of inhalation anesthesia (INH) and injection anesthesia (INJ) during the perioperative period. We analyzed the medical records of 57 Japanese black calves that underwent laparotomy for umbilical diseases (umbilical hernia and/or umbilical cord disease) between January 2017 and December 2023. The INH group (n = 38) received continuous isoflurane inhalation with pure oxygen as a carrier gas, while the INJ group (n = 19) received only injectable anesthesia (xylazine hydrochloride and pentobarbital sodium). We investigated sex, age at first examination, diagnosis, anesthetic method, anesthetic drugs and dosages, number of rescue doses, hospital stay duration, operation time, and prognosis. The number of rescue doses was defined as the number of times sedatives or analgesics (xylazine hydrochloride, pentobarbital sodium, and butorphanol tartrate) were injected during laparotomy. There were no differences in age at first examination (p = 0.8656) and hospital stay duration (p = 0.4646) between the groups. The INJ group required significantly more rescue doses (p < 0.0001) and had longer operation times (p = 0.0643) compared to the INH group. Postoperative prognosis was not significantly different between groups (p = 0.7026). Overall, INJ required multiple rescue doses, but the difference in the method of general anesthesia did not affect the hospital stay duration or prognosis.</p>","PeriodicalId":23690,"journal":{"name":"Veterinary Research Communications","volume":"49 3","pages":"137"},"PeriodicalIF":1.8,"publicationDate":"2025-03-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143606449","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Alleviation of glyphosate-induced toxicity by Horseradish tree (Moringa oleifera) Leaf extract and phytase in Nile Tilapia (Oreochromis niloticus) highlighting the antioxidant, anti-inflammatory, and anti-apoptotic activities.","authors":"Esraa A Elahwl, Doaa H Assar, Ibrahim I Al-Hawary, Abdallah S Salah, Amany E Ragab, Ahmed Elsheshtawy, Mona Assas, Haitham G Abo-Al-Ela, Alamira Marzouk Fouad, Zizy I Elbialy","doi":"10.1007/s11259-025-10672-5","DOIUrl":"10.1007/s11259-025-10672-5","url":null,"abstract":"<p><p>The danger posed by waterborne toxicity from herbicides endangers the aquatic ecosystem. Using dietary medicinal herbs is a useful approach to mitigate the effects of herbicide toxicity on aquatic animals. This study attempts to examine the consequences and potential mechanisms behind the dietary addition of horseradish tree (Moringa oleifera) leaf extract (MOLE) with the help of phytase addition to check the overall growth performance, biochemical changes, histological alteration, and gene expression in normal and after glyphosate challenge in Nile tilapia. A total number of 135 Nile tilapia fish (7.93 ± 0.03 g) were randomly assigned into three groups each in triplicate. The first group is the control group and fed basal diet; the second group supplied with MOLE (200 mg of extract/kg), and the third group was supplied with MOLE (200 mg /kg), and phytase (0.2g/ kg) for 8 weeks. After the feeding trial, each experimental group was divided into two subgroups to be unchallenged and challenged with glyphosate (30 mg/L of water). The results declared significant enhancements (P < 0.05) in Weight Gain Percent (WG%), Specific growth rate (SGR), and Protein efficiency ratio (PER) and reducing feed conversion ratio (FCR) with up-regulating hepatic gh, igf1,myogenine, intestinal ghrelin and NPY in fish groups fed MOLE and phytase compared with the control group. Moreover, improving the hepatic antioxidant capacity while down-regulating hepatic igf1bp, myostatin. Interstingly, MOLE and phytase lightened glyphosate-induced biochemical alterations, antioxidants, apoptosis, and inflammation-associated genes compared to the glyphosate-challenged group. Interestingly, UPLC-ESI-MS/MS analysis recognized 16 compounds encompasing two glucosinolates, three flavonoids, one phenolic and three alkaloids in addition to four fatty acids, a terpenoid, one phytate and an aromatic glycoside. These components might be accountable for the potential effects exerted by MOLE. Therefore, the current study suggests that dietary supplementation to MOLE and phytase can be used as substitute feed supplements in sustainable farming of Nile tilapia to defend against glyphosate challenges and enhance growth, antioxidant capacity, exerting anti-inflammatory and antiapoptotic effects under normal health conditions or post glyphosate challenge.</p>","PeriodicalId":23690,"journal":{"name":"Veterinary Research Communications","volume":"49 3","pages":"135"},"PeriodicalIF":1.8,"publicationDate":"2025-03-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11893658/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143597834","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Progress in diagnostic methods and vaccines for lumpy skin disease virus: a path towards understanding the disease.","authors":"Tarek Korany Farag, Hala A A Abou-Zeina, Sobhy Abdel-Shafy, Ahmad M Allam, Alaa A Ghazy","doi":"10.1007/s11259-025-10667-2","DOIUrl":"10.1007/s11259-025-10667-2","url":null,"abstract":"<p><p>Lumpy skin disease (LSD) is caused by Lumpy Skin disease virus (LSDV) belonging to the genus Capripoxvirus (CaPV). The disease is widespread in Africa, the Middle East and Asia and has been present in Egypt since 1988. LSD is mainly transmitted by blood-sucking insects. LSD is clinically distinguished by a high fever, skin nodules, and swollen Lymph nodes. Detecting sub-clinical disease can be challenging however, prompt laboratory investigations are vital. Skin lesions are the main source of infection, although the virus is shed through many excretions and discharges including semen. Disease confirmation in clinical laboratories includes detection of viral nucleic acid, antigen and antibody levels. Simple, adaptable, and quick assays for detecting LSDV are required for control measures. Vaccination, together with controlled quarantine and vector control measures, may be beneficial for preventing disease spread. Presently, a range of live attenuated vaccines, have been used in the field with different levels of protection and side effects. With high levels of vaccination coverage, attenuated Neethling vaccines have successfully eradicated of LSDV in Europe. Inactivated LSDV vaccines have also been demonstrated effective in experimental infections. Furthermore, due to its large genome, LSDV is being exploited as a vaccine delivery element, generating an innovative composite with additional viral genes by DNA recombination. Vaccines developed on this basis have the potential to prevent a wide range of diseases and have been demonstrated to be effective in experimental settings. In this review, we emphasizethe advances in diagnostic methods and vaccines developed last decade, thereby providing a basis for future research into various aspects of LSDV and providing information for possibility of disease elimination.</p>","PeriodicalId":23690,"journal":{"name":"Veterinary Research Communications","volume":"49 3","pages":"134"},"PeriodicalIF":1.8,"publicationDate":"2025-03-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11890411/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143582368","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Application of ozone during incubation period: hatchability, chick quality and organ growth, bacterial load of feces, and first-week performance in broilers.","authors":"Bilgehan Yılmaz Dikmen, Arda Sözcü, Aydın İpek","doi":"10.1007/s11259-025-10702-2","DOIUrl":"10.1007/s11259-025-10702-2","url":null,"abstract":"<p><p>This study was aimed to investigate the effects of ozone (O₃) treatment during incubation period (IP) on hatchability, hatch window, chick quality and organ growth, bacterial load of feces and first-week growth performance in broilers. A total of 240 hatching eggs were weighed and randomly divided into control group (O₃-IP (-)) and O₃ treatment (O₃-IP (+)). A commercial O₃ generator was placed into the setter and O₃ treatment (at the level of 0.050 ppm) was applied during 1 min per hour in a cyclic period of 3 days during the 18-day incubation period. The egg weight loss between 1 and 18 days ranged with values 8.59% in O₃-IP (-) and 10.63% in O₃-IP (+) group. The pipping time and incubation length was determined as 500.67 h and 527.33 h in O₃-IP (-) and 489.67 h and 518.33 h in O₃-IP (+) respectively. The yolk sac weight was found to be higher in the O₃-IP (-) group compared to the O₃-IP (+). In conclusion, O₃ treatment during incubation period seems to be cause an acceleration for pipping time and shortening of total incubation period, unsteady effects for chick growth and quality, inhibitory effect for bacterial growth in feces.</p>","PeriodicalId":23690,"journal":{"name":"Veterinary Research Communications","volume":"49 3","pages":"133"},"PeriodicalIF":1.8,"publicationDate":"2025-03-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11890230/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143582367","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Anatomopathological characterization of hepatocellular carcinoma in a jaguar (Panthera onca).","authors":"Fernanda Barthelson Carvalho de Moura, Luíz Guilherme Dercore Benevenuto, Noeme Sousa Rocha, Didier Quevedo Cagnini","doi":"10.1007/s11259-025-10708-w","DOIUrl":"https://doi.org/10.1007/s11259-025-10708-w","url":null,"abstract":"<p><p>A 21-year-old jaguar (Panthera onca) with a low body score, pale ocular mucosa, severe dehydration, and decubitus eschar was referred to our veterinary hospital. The patient died and underwent a postmortem examination to establish a diagnosis. Macroscopic evaluation revealed a reddish-black, well-defined, focally extensive mass measuring 12 cm in diameter on the visceral surface involving the left lateral lobe of the liver. The mass was surrounded by a capsule and exhibited diffuse hemorrhage, cystic areas, infiltrative zones on cross-section, and areas of morphologically compatible fatty liver degeneration. The primary differential diagnoses included adenoma, hepatocellular carcinoma, and cholangiocarcinoma. Microscopic analysis showed malignant hepatocytes forming irregular trabeculae of two or more cells, with vascular spaces between them, rudimentary acini, and neoplastic cell aggregates scattered throughout the parenchyma. Additional findings included coagulative necrosis, macrophage infiltration, hepatocyte cord formation, moderate pleomorphism, anisokaryosis, and the presence of discrete binucleated and multinucleated cells. To our knowledge, this is the first report of hepatocellular carcinoma in a jaguar. This information may aid veterinary pathologists in reporting hepatic alterations and characterizing anatomopathological findings in jaguars, representing a valuable contribution to the literature.</p>","PeriodicalId":23690,"journal":{"name":"Veterinary Research Communications","volume":"49 3","pages":"131"},"PeriodicalIF":1.8,"publicationDate":"2025-03-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143574119","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}