Veterinary Research Communications最新文献_第3页

Co-infection with systemic candidiasis and diphtheritic aspergillosis in a Buffalo calf. 水牛小牛的系统性念珠菌病和白喉曲霉病合并感染。

IF 2 3区农林科学

Veterinary Research Communications Pub Date : 2025-09-26 DOI: 10.1007/s11259-025-10885-8

Sumit S Kamble, Veena R Sutar, S D Vinay Kumar, Sonu S Nair, V Athira, J Pranathi, Pawan Kumar, Prasad Thomas, Bablu Kumar, Premanshu Dandapat, Vidya Singh, Abhishek

{"title":"Co-infection with systemic candidiasis and diphtheritic aspergillosis in a Buffalo calf.","authors":"Sumit S Kamble, Veena R Sutar, S D Vinay Kumar, Sonu S Nair, V Athira, J Pranathi, Pawan Kumar, Prasad Thomas, Bablu Kumar, Premanshu Dandapat, Vidya Singh, Abhishek","doi":"10.1007/s11259-025-10885-8","DOIUrl":"https://doi.org/10.1007/s11259-025-10885-8","url":null,"abstract":"Mycotic infections in neonatal calves are often undiagnosed but pose significant risks, especially in immunocompromised neonates. In this study, we report a rare case of co-infection with systemic candidiasis and diphtheritic aspergillosis in a 13-day-old female Murrah buffalo calf presented for necropsy at ICAR-Indian Veterinary Research Institute, Izatnagar, Bareilly. Gross examination revealed pneumonic changes with a well-demarcated nodule in the right caudal pulmonary lobe. Adherent mycotic pseudo membranes over the tracheal mucosa, and type I b haemorrhagic ulcers in the abomasum were also detected. Histopathological examination revealed fungal pseudo-hyphae, budding yeasts within pulmonary microabscesses, bronchiolar epithelium, and vessels, along with angioinvasion. The abomasum exhibited transmural necrotizing mycotic abomasitis with fungal hyphae invading submucosal vessels. Aseptically collected heart blood and lung tissue samples cultured on Sabouraud Dextrose Agar (SDA) yielded C. tropicalis. Tracheal swabs inoculated on SDA revealed growth of Aspergillus fumigatus and Aspergillus flavus. This study highlights that in calves with immature immune systems, opportunistic fungal infections are more likely, and the risk is further heightened by injudicious alteration of antibiotic regimens and corticosteroid therapy; such practices should be avoided to prevent mixed infections.","PeriodicalId":23690,"journal":{"name":"Veterinary Research Communications","volume":"49 6","pages":"331"},"PeriodicalIF":2.0,"publicationDate":"2025-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145151015","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Development and application of a novel indirect ELISA based on recombinant ORFV114 protein for serodiagnosis of Orf virus in goats. 重组ORFV114蛋白间接ELISA检测山羊口蹄疫病毒的建立与应用

IF 2 3区农林科学

Veterinary Research Communications Pub Date : 2025-09-25 DOI: 10.1007/s11259-025-10881-y

Shaobo Liang, Jingjin Hu, Weijie Zhou, Feng Pang

{"title":"Development and application of a novel indirect ELISA based on recombinant ORFV114 protein for serodiagnosis of Orf virus in goats.","authors":"Shaobo Liang, Jingjin Hu, Weijie Zhou, Feng Pang","doi":"10.1007/s11259-025-10881-y","DOIUrl":"https://doi.org/10.1007/s11259-025-10881-y","url":null,"abstract":"Orf, resulting from infection with the Orf virus (ORFV), is a zoonotic disease that threatens the health of both animals and people. Reliable serological detection methods are critical for managing and preventing Orf outbreaks. The ORFV114 protein, highly conserved across ORFV strains, serves as a promising antigen for diagnostic applications. In this study, we developed a novel indirect ELISA for ORFV serodiagnosis using a recombinant ORFV114 protein. The antigen was produced in Escherichia coli via the pET-32a-ORFV114 plasmid, then purified and refolded for use in the assay. Receiver operating characteristic (ROC) analysis of sera from 40 ORFV-negative and 30 ORFV-positive goats established an optimal cut-off value of 0.2938. At this threshold, the assay achieved a diagnostic sensitivity of 93.33% and a specificity of 95%, with an excellent area under the curve (AUC) of 0.9871 (P < 0.0001). The ELISA demonstrated high specificity, with no cross-reactivity against FMDV, GTPV, or PPRV, and showed good reproducibility (CV < 10%). Testing 168 serum samples from asymptomatic goats with the assay showed a 16.67% seropositivity rate for ORFV and an approximate 93.45% concordance with the virus neutralization test, demonstrating its practical applicability. This newly established indirect ELISA assay is a sensitive and specific tool for ORFV surveillance.","PeriodicalId":23690,"journal":{"name":"Veterinary Research Communications","volume":"49 6","pages":"330"},"PeriodicalIF":2.0,"publicationDate":"2025-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145138918","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Response of broiler chickens to dietary different yeast cell wall components challenged with Salmonella typhimurium. 鼠伤寒沙门菌攻毒肉鸡对饲粮不同酵母细胞壁成分的反应。

IF 2 3区农林科学

Veterinary Research Communications Pub Date : 2025-09-25 DOI: 10.1007/s11259-025-10880-z

Pinar Sacakli, Elsayed Mickdam, Alev Gurol Bayraktaroglu, Burcu Ekim, Ozge Sizmaz

{"title":"Response of broiler chickens to dietary different yeast cell wall components challenged with Salmonella typhimurium.","authors":"Pinar Sacakli, Elsayed Mickdam, Alev Gurol Bayraktaroglu, Burcu Ekim, Ozge Sizmaz","doi":"10.1007/s11259-025-10880-z","DOIUrl":"https://doi.org/10.1007/s11259-025-10880-z","url":null,"abstract":"This study assessed the impact of dietary prebiotic supplementation on broiler chickens challenged with Salmonella Typhimurium. In experiment I, the treatments consisted of control (K) [without supplementation]; supplemented with 0.565% autolyzed whole yeast cell (W); supplemented with 0.125% autolyzed whole yeast cell (WP); supplemented with 0.565% enzymatically removed yeast cell wall (CELL) and supplemented with 0.2% pure Mannan-oligosaccharides (MOS) + β-glucan (MB). The second experiment, the Salmonella Typhimurium challenge was performed on day 7 post-hatch. Birds in the CELL group in Experiment I exhibited higher final body weight and weight gain (P = 0.001). On day 21, jejunum villus height (VH) was lower in the MB group, while jejunum crypt depth (CD) was lower in the WP and K groups (P < 0.05). The W group had the lowest VH and CD in the ileum (P < 0.05). At day 35, jejunal villus health was unaffected by prebiotic supplementation, but the CELL group showed the highest VH and CD in the ileum (P < 0.05). Gene expression analysis revealed significant differences in claudin, IL-1β, and IL-10 expressions. In experiment II, the CELL group exhibits the highest final live body weight and weight gain (P < 0.05). Intestinal histology varied among groups and time points. Gene expression analysis indicated upregulation of claudin and occludin in certain groups, while IL-1β was upregulated in all groups and IL-10 showed varied expressions. In conclusion, prebiotic supplementation, particularly yeast cell wall, positively influenced broiler performance, intestinal histology, and gene expression related to the intestinal barrier.","PeriodicalId":23690,"journal":{"name":"Veterinary Research Communications","volume":"49 6","pages":"329"},"PeriodicalIF":2.0,"publicationDate":"2025-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145138921","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Serological, molecular, and epidemiological assessment of Leishmania spp. in equids within a hotspot endemic area for cutaneous leishmaniasis in southeastern Algeria. 阿尔及利亚东南部皮肤利什曼病流行热点地区马科动物中利什曼原虫的血清学、分子和流行病学评估

IF 2 3区农林科学

Veterinary Research Communications Pub Date : 2025-09-25 DOI: 10.1007/s11259-025-10904-8

Meriem Djellouli, Naouel Eddaikra, Assia Beneldjouzi, Razika Benikhlef, Ramjee Ghimire, Melinda Wilkins, Ismail Lafri

{"title":"Serological, molecular, and epidemiological assessment of Leishmania spp. in equids within a hotspot endemic area for cutaneous leishmaniasis in southeastern Algeria.","authors":"Meriem Djellouli, Naouel Eddaikra, Assia Beneldjouzi, Razika Benikhlef, Ramjee Ghimire, Melinda Wilkins, Ismail Lafri","doi":"10.1007/s11259-025-10904-8","DOIUrl":"https://doi.org/10.1007/s11259-025-10904-8","url":null,"abstract":"Cutaneous leishmaniosis remains a major public health concern in Algeria, predominantly affecting humans and dogs. However, the role of other animal hosts, particularly equids, in the transmission cycle remains underexplored. This cross-sectional study presents the first systematic investigation of Leishmania spp. exposure and infection in equids, along with associated environmental and host factors, from southeastern Algeria, El Oued Wilaya, the third most affected region for human cutaneous leishmaniasis in the country. A total of 122 equids from eight communes were clinically examined and tested using three diagnostic tools: indirect fluorescent antibody test, conventional PCR targeting the ITS1 region, and real-time qPCR targeting the kDNA minicircle. Despite the absence of clinical symptoms, serological analysis revealed a 22.13% seropositivity rate for anti-Leishmania infantum IgG. ITS1-PCR and kDNA qPCR detected parasite DNA in 13.93% and 7.38% of samples, respectively. PCR-RFLP analysis confirmed the presence of Leishmania (Leishmania) infantum. Phylogenetic analysis revealed intraspecific divergence among isolates, with two sequences clustering in a distinct clade closely related to a Brazilian strain, indicating unexpected genetic diversity among Algerian isolates. Key associated factors included proximity to human settlements and age-related susceptibility. The detection of subclinical infections in equids suggests a potential but underrecognized epidemiological role in endemic areas. Although their reservoir competence remains unconfirmed, this evidence warrants further research on vector competence, molecular characterization, and entomological surveillance. Integrating equids into One Health surveillance frameworks could help clarify their role and guide targeted control strategies in Algeria where transmission dynamics of leishmaniosis may vary.","PeriodicalId":23690,"journal":{"name":"Veterinary Research Communications","volume":"49 6","pages":"328"},"PeriodicalIF":2.0,"publicationDate":"2025-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145138941","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Low efficacy of levamisole for Ascaridia galli control in experimentally infected poultry: first report in Cajamarca (Peru). 左旋咪唑对实验感染家禽的加利蛔虫控制效果低：在卡哈马卡（秘鲁）首次报道。

IF 2 3区农林科学

Veterinary Research Communications Pub Date : 2025-09-24 DOI: 10.1007/s11259-025-10868-9

Delia Zavala, Juan Rojas-Moncada, Cristian Hobán, Teófilo Torrel, Luis Vargas-Rocha

{"title":"Low efficacy of levamisole for Ascaridia galli control in experimentally infected poultry: first report in Cajamarca (Peru).","authors":"Delia Zavala, Juan Rojas-Moncada, Cristian Hobán, Teófilo Torrel, Luis Vargas-Rocha","doi":"10.1007/s11259-025-10868-9","DOIUrl":"https://doi.org/10.1007/s11259-025-10868-9","url":null,"abstract":"Chickens play an essential role in the global economy and household nutrition. The farming system, particularly extensive or backyard production, affects their productivity due to intestinal parasites, with Ascaridia galli being the most prevalent. Various active compounds are utilized to mitigate its impact; however, improper administration frequently leads to resistant parasites. In some instances, efficacy assessments are not conducted, leaving the effectiveness of different drugs uncertain. This study aimed to evaluate the clinical and controlled efficacy of levamisole in poultry experimentally infected with A. galli in Cajamarca, Peru. Thirty Gallus gallus domesticus chicks were experimentally infected with 210 embryonated A. galli eggs. After confirming established infection through weekly fecal egg counts, 20 birds with ≥ 100 EPG were selected and randomly assigned to control (n = 10) and treatment (n = 10) groups. The treatment group received a single oral dose of levamisole (30 mg/kg body weight). Efficacy was determined by calculating the percentage reduction in egg counts and adult worm burden at day 7 post-treatment. The clinical efficacy between the control and treatment groups was 81.25% (95% CI: 79.63-82.87), the clinical efficacy between pre- and post-treatment was 79.61% (95% CI: 77.87-81.35), and the controlled efficacy was 71.07% (95% CI: 55.74-88.40). No statistically significant differences were observed between the formulas used to determine clinical efficacy (P > 0.01). In conclusion, levamisole administered at 30 mg/kg was insufficiently effective against A. galli in poultry under experimental conditions in Cajamarca, suggesting potential resistance and the need for alternative control strategies.","PeriodicalId":23690,"journal":{"name":"Veterinary Research Communications","volume":"49 6","pages":"327"},"PeriodicalIF":2.0,"publicationDate":"2025-09-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145132054","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Decay of maternal neutralizing antibodies against foot and mouth disease virus (FMDV) in the calves born to the dam vaccinated for FMD during 80-110 days of gestation. 在妊娠80-110天接种口蹄疫疫苗的母鼠出生的小牛中，母鼠抗口蹄疫病毒（FMDV）中和抗体的衰减。

IF 2 3区农林科学

Veterinary Research Communications Pub Date : 2025-09-24 DOI: 10.1007/s11259-025-10876-9

P V Kishor, B H Manjunatha Patel, Sughra Banu, Tamil Selvan Ramasamy Periyasamy, Hollabasappa Teggi, Jeyakumar Sakthivel, Aadhithya Muthuswamy Jayaprakash, Dechamma Hosur Joyappa, Madhusudan Hosamani, Priyanka Mahadappa, Mukesh Singh, Pallab Chaudhuri, Narayanan Krishnaswamy

{"title":"Decay of maternal neutralizing antibodies against foot and mouth disease virus (FMDV) in the calves born to the dam vaccinated for FMD during 80-110 days of gestation.","authors":"P V Kishor, B H Manjunatha Patel, Sughra Banu, Tamil Selvan Ramasamy Periyasamy, Hollabasappa Teggi, Jeyakumar Sakthivel, Aadhithya Muthuswamy Jayaprakash, Dechamma Hosur Joyappa, Madhusudan Hosamani, Priyanka Mahadappa, Mukesh Singh, Pallab Chaudhuri, Narayanan Krishnaswamy","doi":"10.1007/s11259-025-10876-9","DOIUrl":"https://doi.org/10.1007/s11259-025-10876-9","url":null,"abstract":"Passive immunity against foot and mouth disease virus (FMDV) is important till the time of primary vaccination as it causes high mortality in the calf. Hence, it is necessary to determine the vaccination window of the dam during the pregnancy. Accordingly, serial sera were collected at monthly interval (0-5 months) from the calves (n = 15) whose dams were last vaccinated during 80-110 days of pregnancy with an oil-adjuvanted inactivated FMD vaccine covering serotypes O, A, and Asia1. First blood sample from the calf was collected 2-3 days post-birth while blood sample was collected from the dam 3-5 days before expected calving. Virus neutralization (VN) test was performed and the resulting neutralizing antibody titer was compared with the expected protective titer. One sample t test revealed that the calves had passive immunity at birth for all the three serotypes (P > 0.05). Mixed model ANOVA indicated that the VN titer declined significantly by 2 months for serotype O and by 3 months for other two serotypes (P < 0.05). Antibody response to serotype O was highly variable among the calves as evidenced by a random effect of 73%. Though a positive correlation was expected in the VN titer between the dam and calf, no such result was found for any of the three FMDV serotypes. It was concluded that the protective window of FMD vaccination of dam is beyond 110 days for all the three serotypes of FMDV to confer adequate passive immunity to the calf.","PeriodicalId":23690,"journal":{"name":"Veterinary Research Communications","volume":"49 6","pages":"326"},"PeriodicalIF":2.0,"publicationDate":"2025-09-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145131968","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Adjuvant screening of an inactivated Pasteurella multocida vaccine in mice and evaluation of its immunogenicity in sheep. 小鼠多杀性巴氏杆菌灭活疫苗的佐剂筛选及绵羊免疫原性评价。

IF 2 3区农林科学

Veterinary Research Communications Pub Date : 2025-09-23 DOI: 10.1007/s11259-025-10902-w

Cheng-Cai Zhu, Jia-Ying Wu, Ze-Kai Wang, Long-Hao Wang, Liu-Jun Zhang, Kang Yan, Shao-Jun He

{"title":"Adjuvant screening of an inactivated Pasteurella multocida vaccine in mice and evaluation of its immunogenicity in sheep.","authors":"Cheng-Cai Zhu, Jia-Ying Wu, Ze-Kai Wang, Long-Hao Wang, Liu-Jun Zhang, Kang Yan, Shao-Jun He","doi":"10.1007/s11259-025-10902-w","DOIUrl":"https://doi.org/10.1007/s11259-025-10902-w","url":null,"abstract":"This study systematically evaluated the adjuvant efficacy of Rehydragel® LV alum (AL) and Freund's adjuvant in enhancing the immunogenicity of an inactivated Pasteurella multocida vaccine in both murine and ovine models. The mice were immunized with vaccines adjuvanted with either AL and Freund's adjuvant, followed by assessments of humoral and cellular immune responses, organ bacterial burdens, histopathology, and post-challenge survival. Sheep-natural hosts-were immunized with the PM + AL vaccine to validate translational efficacy. The results demonstrated that the PM + AL vaccine elicited significantly higher IgG titers (P < 0.01) and Th2-skewed immune response in mice, characterized by a marked increase in IL-4 and moderate induction of IFN-γ, compared to the Freund's adjuvant. Immunization with the PM + AL vaccine resulted in 100% survival and complete bacterial clearance in organs post-challenge, whereas PM + Freund's vaccine resulted in only 40% survival. Histopathology revealed minimal inflammation in PM + AL vaccinated mice. In sheep, PM + AL vaccination elicited robust IgG responses by day 14, complete protection following challenge, elevated lymphocyte stimulation indices (P < 0.01), and increased serum bactericidal activity, without any observable clinical symptoms. By contrast, sheep in the control group exhibited signs of depression and coughing. These findings underscore the importance of validating adjuvant efficacy in target species and highlight the potential of AL as a promising adjuvant for P. multocida vaccines in sheep.","PeriodicalId":23690,"journal":{"name":"Veterinary Research Communications","volume":"49 6","pages":"322"},"PeriodicalIF":2.0,"publicationDate":"2025-09-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145126120","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Combining network pharmacology and metabonomics to explore the mechanism of baicalin inhibiting PRRSV replication. 结合网络药理学和代谢组学探讨黄芩苷抑制PRRSV复制的机制。

IF 2 3区农林科学

Veterinary Research Communications Pub Date : 2025-09-23 DOI: 10.1007/s11259-025-10899-2

Qin Luo, Yu-Xuan Huang, Hai-Rui Li, Chang-Hong Qiao, Zhen-Wen He, Xiang-Yu Chen, Xiao-Hu Wang, Gang Wang, Meng-Meng Zhao, Ru-Jian Cai

{"title":"Combining network pharmacology and metabonomics to explore the mechanism of baicalin inhibiting PRRSV replication.","authors":"Qin Luo, Yu-Xuan Huang, Hai-Rui Li, Chang-Hong Qiao, Zhen-Wen He, Xiang-Yu Chen, Xiao-Hu Wang, Gang Wang, Meng-Meng Zhao, Ru-Jian Cai","doi":"10.1007/s11259-025-10899-2","DOIUrl":"https://doi.org/10.1007/s11259-025-10899-2","url":null,"abstract":"Porcine reproductive and respiratory syndrome virus (PRRSV) is widely prevalent and causes significant economic losses to the global swine industry. Currently available vaccines provide insufficient protection, and there are no clinically approved antiviral treatments specifically targeting PRRSV. Accordingly, developing safe and potent measures for PRRS prevention and control remains an urgent priority. Yinhuang soluble powder is a traditional Chinese medicinal preparation mainly composed of baicalin and chlorogenic acid, with baicalin serving as its principal active ingredient, which demonstrates significant potential in antiviral research. However, current research on baicalin's anti-PRRSV activity is notably lacking, with its molecular mechanisms still not fully elucidated. In this study, in vitro experiments demonstrated that both Yinhuang soluble powder and baicalin not only effectively inhibited PRRSV replication but also interfered with the viral replication cycle, concurrently demonstrating significant downregulation of pro-inflammatory cytokines. Network pharmacological screening identified eight core targets of baicalin against PRRSV, namely MAPK14, MAPK10, MAPK8, NF-κB1, MAPK1, SRC, IL-6, and TP53. Molecular docking revealed baicalin's strong binding affinity to the core targets, with free binding energies below - 7.0 kcal/mol. In addition, metabolomics identified 29 metabolites associated with the anti-PRRSV effects of baicalin, the main components of which were glycerophospholipids, glycerolipids, and fatty acyls, and the principal metabolic pathways through which baicalin exerts its anti-PRRSV effects are glycerophospholipid and glycerolipid metabolism. In conclusion, this study demonstrates that baicalin exhibits promising anti-PRRSV efficacy, proposing new insights for PRRS prevention and treatment.","PeriodicalId":23690,"journal":{"name":"Veterinary Research Communications","volume":"49 6","pages":"323"},"PeriodicalIF":2.0,"publicationDate":"2025-09-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145126083","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Demonstrating persistence of foot-and-mouth disease virus in African buffalo (Syncerus caffer) using BaseScope™ in situ hybridisation. 利用BaseScope™原位杂交技术证明非洲水牛（Syncerus caffer）中口蹄疫病毒的持久性。

IF 2 3区农林科学

Veterinary Research Communications Pub Date : 2025-09-23 DOI: 10.1007/s11259-025-10898-3

Alischa Henning, Lieza Odendaal, Angelika Loots, Melvyn Quan

{"title":"Demonstrating persistence of foot-and-mouth disease virus in African buffalo (Syncerus caffer) using BaseScope™ in situ hybridisation.","authors":"Alischa Henning, Lieza Odendaal, Angelika Loots, Melvyn Quan","doi":"10.1007/s11259-025-10898-3","DOIUrl":"10.1007/s11259-025-10898-3","url":null,"abstract":"BaseScope™ in situ hybridisation (Advanced Cell Diagnostics, USA) was used to detect foot-and-mouth disease virus (FMDV), species Aphthovirus vesiculae, in formalin-fixed paraffin-embedded tissues from African buffalo (Syncerus caffer, n = 15) culled from the Kruger National Park - where the South African territories (SAT)-1, -2 and - 3 serotypes are endemic - as part of their population management program. Foot-and-mouth disease viral RNA was consistently detected in the palatine tonsils and lungs, demonstrating these as primary sites of viral persistence. Detection in the retropharyngeal lymph nodes and interdigital skin was less frequent, while oropharyngeal tissue showed rare positivity. Other sampled tissues - including the tip of the ear, eyelid, tongue, lip, and coronary band - proved suboptimal for identifying FMDV-positive buffalo or carriers. These findings highlight the value of BaseScope™ for detecting low viral loads of FMDV in persistently infected African buffalo, with a notable predilection for the palatine tonsils and lungs.","PeriodicalId":23690,"journal":{"name":"Veterinary Research Communications","volume":"49 6","pages":"324"},"PeriodicalIF":2.0,"publicationDate":"2025-09-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12457507/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145126102","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Clinical, cytological, and microbiological evaluation of a topical boric acid-alcohol solution for the treatment of canine otitis externa: a randomized, open-label controlled clinical trial. 局部硼酸-酒精溶液治疗犬外耳炎的临床、细胞学和微生物学评价：一项随机、开放标签对照临床试验。

IF 2 3区农林科学

Veterinary Research Communications Pub Date : 2025-09-23 DOI: 10.1007/s11259-025-10903-9

Elif Ünlü

{"title":"Clinical, cytological, and microbiological evaluation of a topical boric acid-alcohol solution for the treatment of canine otitis externa: a randomized, open-label controlled clinical trial.","authors":"Elif Ünlü","doi":"10.1007/s11259-025-10903-9","DOIUrl":"10.1007/s11259-025-10903-9","url":null,"abstract":"Otitis externa (OE) is a common inflammatory condition frequently encountered in veterinary practice. This study aimed to evaluate the efficacy of a topical 5% boric acid-alcohol (BAA) solution compared to a commercial ear cleanser (EpiOtic®) for treating canine OE. Thirty client-owned dogs with OE were enrolled in a randomized, open-label controlled clinical trial and assigned to two treatment groups: Group I received BAA combined with EpiOtic®, and Group II received EpiOtic® alone, both applied twice daily for 14 days. Clinical assessments-including OTIS-3 scoring, secretion type, otoscopic findings, pain, pruritus, and cytology-were performed on days 0, 7, 14, and 21, with microbiological analyses on days 0 and 21. By day 21, treatment success was achieved in 93.3% of Group I dogs, compared to 26.7% in Group II (p < 0.001). Group I showed significant improvements in all clinical parameters (p ≤ 0.001), while Group II exhibited only modest changes. Cytology showed significantly better results in Group I on days 7 (p = 0.002), 14, and 21 (both p < 0.001), with cytological success rates of 93.3% vs. 20% in Group II. By day 21, microbiological analysis demonstrated complete fungal eradication and near-complete bacterial clearance in Group I, with cultures confirming elimination of common pathogens including Staphylococcus intermedius, Proteus mirabilis, Escherichia coli, Staphylococcus aureus, Staphylococcus epidermidis, and Malassezia pachydermatis. These findings indicate that BAA is a safe, effective, and economical alternative for managing canine OE and may contribute to antimicrobial stewardship by reducing the need for systemic therapies.","PeriodicalId":23690,"journal":{"name":"Veterinary Research Communications","volume":"49 6","pages":"325"},"PeriodicalIF":2.0,"publicationDate":"2025-09-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145126137","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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