Veterinary Research Communications最新文献_第4页

Molecular detection of Bartonella in bats and their ectoparasites, Spinturnix myoti, from central and Western Yunnan Province, China.

IF 1.8 3区农林科学

Veterinary Research Communications Pub Date : 2025-03-07 DOI: 10.1007/s11259-025-10704-0

Xiaoyan Zheng, Xianzheng Zhang, Yifan Deng, Ying Li, Yiru Gu, Xiaobin Huang

{"title":"Molecular detection of Bartonella in bats and their ectoparasites, Spinturnix myoti, from central and Western Yunnan Province, China.","authors":"Xiaoyan Zheng, Xianzheng Zhang, Yifan Deng, Ying Li, Yiru Gu, Xiaobin Huang","doi":"10.1007/s11259-025-10704-0","DOIUrl":"https://doi.org/10.1007/s11259-025-10704-0","url":null,"abstract":"Bartonella, a facultative intracellular pathogen, is known for causing zoonotic diseases and has been detected in a variety of mammals and arthropods. Previous research has highlighted bats as natural reservoirs for Bartonella, with bat ectoparasites acting as vectors for transmission. Moreover, studies have indicated a positive correlation between the prevalence of ectoparasites and Bartonella infection levels. However, research on Bartonella carried by bats and their ectoparasites in China is limited. In this study, we captured bats at three sites in Yunnan Province, China, and collected their ectoparasites. Using conventional PCR, we screened for Bartonella by targeting three gene fragments: the RNA polymerase subunit gene (rpoB), citrate synthase (gltA), and cell division protein (ftsZ). Further phylogenetic and genetic distance analyses indicated the presence of identical Bartonella strains in bats and their ectoparasitic mites, suggesting the high risk of bat hosts and the potential of their ectoparasites as vectors for pathogen transmission. Despite this, the mechanisms underlying the maintenance and transmission of Bartonella within bat and ectoparasite populations, as well as the process of spillover to humans, remain poorly understood. This underscores the need for further in-depth experimental studies on mammals and their ectoparasites.","PeriodicalId":23690,"journal":{"name":"Veterinary Research Communications","volume":"49 3","pages":"132"},"PeriodicalIF":1.8,"publicationDate":"2025-03-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143574120","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Validation and comparison of EvaGreen- and TaqMan-based real-time qRT-PCR for diagnosis of the black queen cell virus in the honey bees.

IF 1.8 3区农林科学

Veterinary Research Communications Pub Date : 2025-03-06 DOI: 10.1007/s11259-025-10706-y

Mustafa Emin Oz, Oguzhan Avci

{"title":"Validation and comparison of EvaGreen- and TaqMan-based real-time qRT-PCR for diagnosis of the black queen cell virus in the honey bees.","authors":"Mustafa Emin Oz, Oguzhan Avci","doi":"10.1007/s11259-025-10706-y","DOIUrl":"https://doi.org/10.1007/s11259-025-10706-y","url":null,"abstract":"The black queen cell virus (BQCV) is a common agent that causes covert infection in hives and has a global distribution. In this study, TaqMan probe and EvaGreen (EG) dye-based real-time quantitative reverse transcription polymerase chain reaction (qRT-PCR) assays based on the amplification of coding senquences for the BQCV structural protein (SP) and the non-structural protein (NSP) were developed and validated for the detection of BQCV in adult bee and larvae/pupae. Additionally, the performances of commonly used EG and TaqMan chemicals for real-time qRT-PCR analyses were compared. The operating range for BQCV-SP TaqMan and EG real-time qRT-PCRs ranged 2.61-7.61 log10 RNA copies/reaction and 2.61-7.79 log10 RNA copies/reaction, respectively. The operating range for BQCV-NSP TaqMan and EG real-time qRT-PCRs ranged 6.83-11.83 log10 RNA copies/reaction and 6.98-11.98 log10 RNA copies/reaction, respectively. Based on these novel assays, the prevalence of BQCV in adult bees and larvae/pupae was 84% (88/105) and 44% (46/105), respectively. BQCV viral load was also within the operating range determined during assay validation. Comparable results were obtained in analytical and diagnostic performance analyses of the EG dye and TaqMan probe chemicals. Highly sensitive and analytically specific for the detection of BQCV, these real-time qRT-PCR assays will benefit the etiological and epidemiological studies of BQCV.","PeriodicalId":23690,"journal":{"name":"Veterinary Research Communications","volume":"49 3","pages":"130"},"PeriodicalIF":1.8,"publicationDate":"2025-03-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143568233","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The first report of gastro-thoracoschisis in a puppy born to a pomeranian bitch afflicted with dystocia.

IF 1.8 3区农林科学

Veterinary Research Communications Pub Date : 2025-03-05 DOI: 10.1007/s11259-025-10681-4

Emadeddin Mobedi, Hamid Ghasemzadeh-Nava, Sara Shokrpoor, Massoud Talebkhan Garoussi, Vahid Akbarinejad, Ali Shojaei, Hassan Zaheri-Abdehvand

{"title":"The first report of gastro-thoracoschisis in a puppy born to a pomeranian bitch afflicted with dystocia.","authors":"Emadeddin Mobedi, Hamid Ghasemzadeh-Nava, Sara Shokrpoor, Massoud Talebkhan Garoussi, Vahid Akbarinejad, Ali Shojaei, Hassan Zaheri-Abdehvand","doi":"10.1007/s11259-025-10681-4","DOIUrl":"https://doi.org/10.1007/s11259-025-10681-4","url":null,"abstract":"A 1.5-year-old primiparous Pomeranian bitch was referred with abdominal discomfort and clinical signs of parturition. Fetal membranes were hanging from vulva, yet no evident progress in parturition was observed. Following medical treatment of the bitch with calcium and oxytocin, the first puppy was born, which had protruding viscera. The abnormal puppy was diagnosed with gastro-thoracoschisis as it has a thoracoabdominal cleft from sternum to pubis, but without skeletal abnormalities. Further, it was revealed that there were some histopathological lesions in the liver and lung of the abnormal puppy. The second and third puppies delivered without difficulty, but only one of these puppies was alive at birth. This case represents the first report of gastro-thoracoschisis clinical case in Pomeranian breed. This case underscores the complexities of managing congenital anomalies in canine and highlights the need for further research to understand the etiology of GTS, which is observed more frequently in ruminants and sporadically in dogs.","PeriodicalId":23690,"journal":{"name":"Veterinary Research Communications","volume":"49 3","pages":"128"},"PeriodicalIF":1.8,"publicationDate":"2025-03-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143558199","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

High prevalence of Chorioptes bovis: an important factor in chronic progressive lymphedema in Belgian draft horses.

IF 1.8 3区农林科学

Veterinary Research Communications Pub Date : 2025-03-05 DOI: 10.1007/s11259-025-10695-y

M Brys, E Claerebout, V Saey, K Chiers

{"title":"High prevalence of Chorioptes bovis: an important factor in chronic progressive lymphedema in Belgian draft horses.","authors":"M Brys, E Claerebout, V Saey, K Chiers","doi":"10.1007/s11259-025-10695-y","DOIUrl":"10.1007/s11259-025-10695-y","url":null,"abstract":"A cross-sectional study was conducted to estimate the prevalence of chorioptic mange affecting the distal legs of horses in Belgium, focusing on the association between mange and chronic progressive lymphedema (CPL) in Belgian draft horses. Clinical examinations and skin scrapings were performed on the distal legs of 156 Belgian draft horses and 142 Belgian warmblood horses. In the Belgian draft horse breed, 144 (92.31%) horses were infested with Chorioptes bovis mites, and 126 (80.77%) displayed clinical signs of CPL. CPL prevalence in draft horses aged < 1 year was 17.86%, while mites were detected in 85.71% of this age group, with infestations observed as early as 6 days of age. In a subset of horses aged ≥ 1 year, CPL and mange prevalence amounted to 94.53% and 93.75%, respectively. In contrast, no mites or CPL were detected in the Belgian warmblood horses examined. Statistical analysis revealed a strong association between C. bovis and CPL (prevalence odds ratio: 7.37; p = 0.002). The prevalence of CPL was approximately twice as high in horses with mites compared to non-infested horses (prevalence ratio: 2.02). Furthermore, the prevalence risk difference of 42.36%, indicates a substantial absolute increase in CPL prevalence among infested horses. This study demonstrates the high prevalence of C. bovis and its breed-specific predilection in Belgian draft horses. The strong association between mange and CPL highlights the potential role of C. bovis as a contributing factor in CPL pathogenesis.","PeriodicalId":23690,"journal":{"name":"Veterinary Research Communications","volume":"49 3","pages":"129"},"PeriodicalIF":1.8,"publicationDate":"2025-03-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11882615/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143558197","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Correction to: Sublingual and subpharyngeal floor, beak, and palate of the local Egyptian ISA Brown Hen (Gallus gallus Domesticus): gross, morphometric, and scanning electron microscopic features with new insights into their nutritional adaptations.

IF 1.8 3区农林科学

Veterinary Research Communications Pub Date : 2025-03-04 DOI: 10.1007/s11259-025-10699-8

Barakat M Alrashdi, Mousa O Germoush, Ibrahim B Abdel-Farid, Diaa Massoud, Eman Kamal Khalil, Ahmed G Nomir, Mohamed Abumandour

引用次数: 0

Proteomic profiling of the serological response to a chemically-inactivated nodavirus vaccine in European sea bass Dicentrarchus labrax.

IF 1.8 3区农林科学

Veterinary Research Communications Pub Date : 2025-03-04 DOI: 10.1007/s11259-025-10688-x

Nadia Chérif, Kais Ghedira, Houda Agrebi, Semah Najahi, Hiba Mejri, Saifeddine Azouz, Mélodie Kielbasa, Jean Armengaud, Richard Thiga Kangethe, Viskam Wijewardana, Balkiss Bouhaouala-Zahar, Haitham Sghaier

{"title":"Proteomic profiling of the serological response to a chemically-inactivated nodavirus vaccine in European sea bass Dicentrarchus labrax.","authors":"Nadia Chérif, Kais Ghedira, Houda Agrebi, Semah Najahi, Hiba Mejri, Saifeddine Azouz, Mélodie Kielbasa, Jean Armengaud, Richard Thiga Kangethe, Viskam Wijewardana, Balkiss Bouhaouala-Zahar, Haitham Sghaier","doi":"10.1007/s11259-025-10688-x","DOIUrl":"10.1007/s11259-025-10688-x","url":null,"abstract":"The analysis of animal responses to immunization is fundamental to vaccine development, enabling the evaluation of immune responses and the elucidation of key mechanisms underlying protective immunity. Such insights are essential for advancing vaccines through clinical trial stages and regulatory approval. Furthermore, understanding the molecular signatures of approved vaccines not only deepens our knowledge of their function but also guides the rational design and improvement of future vaccines. This study aims to elucidate alterations in protein abundance patterns in the sera of European sea bass, D. labrax, following immunization with a chemically-inactivated nodavirus vaccine. The shotgun proteome comparison revealed that in vaccinated animals, compared to controls, there is a modulation of the redox balance favouring reactive oxygen species (ROS), an intricate interaction between coagulation and the immune system resulting in the lower abundance of hematopoiesis-related FLT3, and indications of functional adaptive immunity demonstrated by the lower abundance of pentraxin fusion protein-like and the over-representation of myosins. To the best of our knowledge, this study represents the inaugural investigation of the immune response in fish using a proteomics approach, employing D. labrax as the host and nodavirus as the pathogen used for vaccination and challenge.","PeriodicalId":23690,"journal":{"name":"Veterinary Research Communications","volume":"49 3","pages":"125"},"PeriodicalIF":1.8,"publicationDate":"2025-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143543686","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Missense variants in MC4R gene are associated with obesity in cats.

IF 1.8 3区农林科学

Veterinary Research Communications Pub Date : 2025-03-04 DOI: 10.1007/s11259-025-10700-4

Salah Aldin Mousa Basha, Iraz Akis

{"title":"Missense variants in MC4R gene are associated with obesity in cats.","authors":"Salah Aldin Mousa Basha, Iraz Akis","doi":"10.1007/s11259-025-10700-4","DOIUrl":"10.1007/s11259-025-10700-4","url":null,"abstract":"Obesity stands out as the most common multifactorial nutritional problem affecting domestic cats. According to studies, the prevalence of overweight or obese cats varies between 11.5% and 63%. Various factors such as breed, age, gender, reproductive status, owner-pet relationship, diet type, and environmental factors have been identified as potential risk factors for the development of obesity in cats. Among the genes involved in regulating energy balance, one of the prominent genes is melanocortin-4 receptor gene (MC4R). A specific missense variant in the feline MC4R gene (c.92 C > T) has been associated with overweight in diabetic domestic shorthaired cats. In this study, it was aimed to determine the polymorphisms in MC4R gene in random bred cats and cats belonging to a registered breed in Turkey and to investigate their relationship with obesity. Blood samples from 30 obese and 20 non-obese cats were collected into sterile vacuum EDTA tubes. Exon 1 of the MC4R was amplified and sequenced. As a result of DNA sequence analysis, we identified a total of six SNPs in the feline MC4R gene, four of which were found for the first time in this study. As a result of comparing allele frequencies in obese and non-obese cats, a significant relationship was found between SNP rs783632116 and obesity. The results of regression analyses evaluating the effects of SNP genotypes, sex and infertility status on feline Body Mass Index (fBMI) indicated that non-synonymous SNPs rs783632116, ss11356259660 and ss11356259661 were significantly associated with fBMI.","PeriodicalId":23690,"journal":{"name":"Veterinary Research Communications","volume":"49 3","pages":"127"},"PeriodicalIF":1.8,"publicationDate":"2025-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11880134/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143543682","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Vestibular syndrome in dairy heifers associated with Mycobacterium bovis: two case reports and strain characterization.

IF 1.8 3区农林科学

Veterinary Research Communications Pub Date : 2025-03-03 DOI: 10.1007/s11259-025-10696-x

Germán J Cantón, Yolanda M Marron, María A Fiorentino, Eleonora L Morrell, Juan A García, María J Marfil, Facundo Urtizbiria, Claudia Morsella, Karina M Cirone, María V Scioli, Martín J Zumárraga, Fernando Paolicchi

{"title":"Vestibular syndrome in dairy heifers associated with Mycobacterium bovis: two case reports and strain characterization.","authors":"Germán J Cantón, Yolanda M Marron, María A Fiorentino, Eleonora L Morrell, Juan A García, María J Marfil, Facundo Urtizbiria, Claudia Morsella, Karina M Cirone, María V Scioli, Martín J Zumárraga, Fernando Paolicchi","doi":"10.1007/s11259-025-10696-x","DOIUrl":"https://doi.org/10.1007/s11259-025-10696-x","url":null,"abstract":"Bovine tuberculosis is a chronic disease caused by Mycobacterium bovis which rarely causes a neurologic clinical presentation. Tuberculous meningitis is a common clinical finding in young calves in dairy herds where tuberculosis is endemic, associated with the early consumption of milk from infected cows. We report two simultaneous cases of vestibular syndrome, including loss of balance, circling, tilted head, and strabismus in 12- and 16-month-old Holstein heifers on a dairy farm in Tandil, Buenos Aires province, Argentina, with low tuberculosis prevalence (< 1.0%). Several widespread granulomatous lesions including large intracranial granulomas, characterized by a fibrous capsule containing pale yellow granular caseous material, with were observed during postmortem examination of both heifers. Microscopically, granulomas were characterized by the presence of a central caseous necrosis with mineralization, surrounded by an abundant macrophages, lymphocytes, and plasma cells infiltrate, with the presence of multiple Langhans-type giant cells, epithelioid macrophages, and connective tissue proliferation in the outer circumference. Mycobacterium bovis was isolated from different granulomas and associated lymph nodes and was typed by spoligotyping. This uncommon clinical presentation of bovine tuberculosis should be considered by veterinary practitioners, particularly in dairy herds where tuberculosis is endemic.","PeriodicalId":23690,"journal":{"name":"Veterinary Research Communications","volume":"49 3","pages":"124"},"PeriodicalIF":1.8,"publicationDate":"2025-03-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143543692","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Unveiling the chewing lice (Menoponidae and Philopteridae) fauna of the Brazilian Northeast: new records for Maranhão and Piauí States.

IF 1.8 3区农林科学

Veterinary Research Communications Pub Date : 2025-03-01 DOI: 10.1007/s11259-025-10694-z

Ricardo Bassini-Silva, Beatriz Bacelar Barbosa, Aryna Dias Pereira, Flávio Kulaif Ubaid, Fernando de Castro Jacinavicius

引用次数: 0

Infection by Syngamidae in seabirds along the Brazilian Coast: new occurrences of Cyathostoma.

IF 1.8 3区农林科学

Veterinary Research Communications Pub Date : 2025-03-01 DOI: 10.1007/s11259-025-10697-w

André Mota Alves, Larissa Mello Figueiredo, Elaine Knupp de Brito, Jociery Einhardt Vergara Parente, Claudia Carvalho do Nascimento, Carlos Alberto Palmeira Sarmento, Iara Gama de Medeiros, Victor Fernando Santana Lima, João Carlos Gomes Borges

{"title":"Infection by Syngamidae in seabirds along the Brazilian Coast: new occurrences of Cyathostoma.","authors":"André Mota Alves, Larissa Mello Figueiredo, Elaine Knupp de Brito, Jociery Einhardt Vergara Parente, Claudia Carvalho do Nascimento, Carlos Alberto Palmeira Sarmento, Iara Gama de Medeiros, Victor Fernando Santana Lima, João Carlos Gomes Borges","doi":"10.1007/s11259-025-10697-w","DOIUrl":"10.1007/s11259-025-10697-w","url":null,"abstract":"This study reports new occurrences of Syngamidae nematodes, specifically Cyathostoma (Cyathostoma) phenisci, in seabirds stranded along the northeastern coast of Brazil. Through systematic monitoring efforts of seabird strandings along the coast of Sergipe (northeastern Brazil), deceased specimens were subjected to post-mortem analyses. During necropsies, nematodes were identified in the tracheae of three specimens (Calonectris borealis, Puffinus puffinus, and Ardenna gravis), indicating respiratory infections. The parasites were collected and identified as C. phenisci. This represents the first record of this species in birds of the order Procellariiformes in Brazil. This study expands the known host range for this parasite and suggests a potential underreporting of respiratory infections caused by Syngamidae in seabirds. The findings underscore the importance of continuous monitoring of these populations and their implications for the health and conservation of migratory birds.","PeriodicalId":23690,"journal":{"name":"Veterinary Research Communications","volume":"49 2","pages":"121"},"PeriodicalIF":1.8,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143531909","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0