Veterinary Research Communications最新文献

{"title":"Potential use of titanium nano-gel to palliate redox status, DNA damage, histopathological changes, and renal-hepatic disorders induced by Candida albicans in Clarias gariepinus.","authors":"Randa Y Thabet, Abdallah Tageldein Mansour, Heba H Mahboub, Yousef Alkhamis, Hesham A Hassanien, Ahmed Abbas, Adnan Alhaje, Sameh H Ismail, Afaf N Abdel Rahman, Heba Allah M Elbaghdady","doi":"10.1007/s11259-025-10882-x","DOIUrl":"10.1007/s11259-025-10882-x","url":null,"abstract":"<p><p>Currently, there has been an obvious direction for making use of green nanotechnology in the aquaculture sector for maintaining fish performance and health. Therefore, the current perspective is a novel attempt to assess the potential use of green-synthesized titanium dioxide nano-gel (GSTG) fabricated by Caralluma fimbriata as a waterborne on African catfish (Clarias gariepinus) physiological performance, histopathological changes, and Candida albicans infection resistance. Fish (77.90 ± 4.02 g) were assigned to four treatments, including control (CON), GSTG, C. albicans, and GSTG + C. albicans. The fish of the CON and GSTG groups were intraperitoneally (IP) inoculated with sterile phosphate buffer saline and exposed to 0.00 and 2.00 mg/L of GSTG, respectively. Meanwhile, the fish of the C. albicans and GSTG + C. albicans groups were IP injected with C. albicans (0.30 × 10⁸ CFU/fish) and treated with 0.00 and 2.00 mg/L of GSTG, respectively, for 10 days. Findings revealed that C. albicans resulted in a reduction in fish survival (62.50%) and an occurrence of oxidative stress response. This is indicated by lower activity of glutathione peroxidase and catalase, and higher levels of lipid peroxide (malonaldehyde) and a product of oxidative DNA damage (8-hydroxy-2-deoxyguanosine). Additionally, an elevation in the stress indices (cortisol and glucose) and renal-hepatic functioning biomarkers (creatinine, aspartate aminotransferase, and alanine aminotransferase) was detected in C. albicans-challenged fish. Moreover, alterations in the histological picture in renal and hepatic tissues were observed, where C. albicans induced necrosis, congestion, degeneration, and inflammatory cell infiltration. Surprisingly, the GSTG application enhanced fish survival to 85.00%, and improved the adverse physiological consequences induced by C. albicans in all measured parameters, besides reviving the injured tissues. Based on the study findings, GSTG exerts a potentially beneficial role in defending C. gariepinus from physiological and health disorders prompted by the C. albicans challenge, and hence, it could be considered a potential remedial measure in the aquaculture sector.</p>","PeriodicalId":23690,"journal":{"name":"Veterinary Research Communications","volume":"49 6","pages":"310"},"PeriodicalIF":2.0,"publicationDate":"2025-09-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145056032","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"New record of the cercariae of liver fluke (Opisthorchis sp.) (Digenea: Opisthorchiidae) from Gabbia kiusiuensis (S. Hirase, 1927) (Caenogastropoda: Bithyniidae) in Shiga prefecture, Japan.","authors":"Shin Kubo, Shigefumi Kanao, Karin Tsuchida, Misako Urabe","doi":"10.1007/s11259-025-10873-y","DOIUrl":"10.1007/s11259-025-10873-y","url":null,"abstract":"<p><p>We investigated two species of bithyniid snails, Gabbia kiusiuensis and Parafossarulus manchouricus japonicus, in Shiga Prefecture, Japan, between 2021 and 2024, to evaluate their infections with cercariae of trematodes. A type of parapleurolophocercous cercariae was detected in G. kiusiuensis from a locality near Lake Biwa. 28S rDNA and cox1 sequence analyses revealed that these cercariae probably belong to the genus Opisthorchis Blanchard, 1895. This is the first record of this genus in snails in Japan. Species of Opisthorchis can be pathogenic to mammals and birds, but the actual pathogenicity of the present species remains unknown. The recent spread of G. kiusiuensis in Japan could lead to cases of liver fluke infections.</p>","PeriodicalId":23690,"journal":{"name":"Veterinary Research Communications","volume":"49 6","pages":"311"},"PeriodicalIF":2.0,"publicationDate":"2025-09-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145055991","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Prevalence of paratuberculosis in Murciano-Granadina dairy goats in Iran: seroprevalence in two large industrial herds and consequences.","authors":"Maziar Kaveh Baghbadorani, Samad Lotfollahzadeh, Abdolrahman Kazemi Hasanvand, Mohammad Hadi Khabazan, Behnam Hajimohammadi Darabi","doi":"10.1007/s11259-025-10869-8","DOIUrl":"10.1007/s11259-025-10869-8","url":null,"abstract":"<p><p>Paratuberculosis is a globally distributed and fatal disease affecting ruminant populations. This study aimed to assess the seroprevalence of paratuberculosis in two large industrial dairy herds of Murciano-Granadina located in Qazvin, and Kerman province, Iran. Serum (N = 1675), and bulk tank milk (N = 2) samples from both herds were analyzed using serum and milk ELISA antibody test, respectively. Several reproductive and productive parameters, including milk yield, services per conception, kidding-to-conception interval, litter size, and gestation length, were compared between seropositive and seronegative goats. The overall apparent seroprevalence differed significantly between two studied herds, which were considered as the control (2.0%, 1/50) and suspected (19.3%, 314/1625) herds (P = 0.002), while the true seroprevalence was estimated at 3.5% and 34.5%, respectively (P < 0.0001). A significant difference in seroprevalence was observed between primiparous does (14.1%) and those with multiple parities (26%) (P < 0.01). Additionally, seropositive goats were significantly older (P = 0.001) and had higher parity (P = 0.003) than seronegative individuals. In terms of production, primiparous seronegative goats produced significantly more milk (P = 0.04) compared to their seropositive counterparts. Seropositive goats also required more services per conception (P = 0.03). The incidence of stillbirths (P = 0.09) and abortions in primiparous seropositive does (P = 0.06) tended to be higher. No other significant differences were observed between the two serogroups. In conclusion, the seroprevalence of paratuberculosis significantly varied. Furthermore, seropositive does, particularly during their first parity exhibited reduced milk production and required more services per conception.</p>","PeriodicalId":23690,"journal":{"name":"Veterinary Research Communications","volume":"49 6","pages":"309"},"PeriodicalIF":2.0,"publicationDate":"2025-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145041452","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"ASF outbreaks in Vietnam (2019-2024): insights and lessons learned.","authors":"Nhat Huy Bui, Thi Trang Vy, Ngoc Bao Anh Ngo, Quoc Khanh Dam, The Viet Hoang Nguyen, Thi Chau Giang Tran, Aruna Ambagala, Van Phan Le","doi":"10.1007/s11259-025-10884-9","DOIUrl":"https://doi.org/10.1007/s11259-025-10884-9","url":null,"abstract":"<p><p>African swine fever (ASF) is a contagious viral disease that affects domestic pigs and Eurasian wild boars, causing significant economic losses to the global pig industry. Since its first outbreak in February 2019, ASF has had a profound impact on the Vietnamese pig sector. This review presents a comprehensive analysis of ASF outbreaks in Vietnam from 2019 to 2024, focusing on outbreak dynamics, control strategies, economic impact, and key lessons learned. By summarizing findings from various studies and official reports, this review provides key insights and practical recommendations to improve ASF management and prevention in Vietnam and across the region.</p>","PeriodicalId":23690,"journal":{"name":"Veterinary Research Communications","volume":"49 6","pages":"307"},"PeriodicalIF":2.0,"publicationDate":"2025-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145030796","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The role of Moringa oleifera in enhancing fish performance and health: a comprehensive review of sustainable aquaculture applications.","authors":"Sohair Y Saleh, Nehal A Younis, Ahmed H Sherif, Howyda G Gaber","doi":"10.1007/s11259-025-10889-4","DOIUrl":"10.1007/s11259-025-10889-4","url":null,"abstract":"<p><p>This comprehensive review examines the versatile applications and effects of Moringa oleifera across multiple fish species in aquaculture systems amid growing challenges of rising feed costs and antimicrobial resistance. M. oleifera, commonly called the Miracle tree, contains an exceptional nutritional profile with high protein content (22.99-29.36% dry weight), complete profiles of essential amino acids, and remarkably elevated levels of vitamins and minerals. Its bioactive profile includes substantial quantities of polyphenols, flavonoids, alkaloids (niazimicin, niaziminin, β-sitosterol), and glucosinolates that collectively enhance fish health and performance. Recent studies across multiple fish species demonstrate variable optimal inclusion rates (5-30%) with specific growth rates increasing 15-35% and feed conversion ratios improving 10-25% compared to controls. Physiological benefits include enhanced digestive enzyme activity, improved liver and kidney functions, and reduced cortisol levels during environmental stress. Immunologically, M. oleifera supplementation significantly increases lysozyme activity, complement C3, total IgM levels, and pro-inflammatory cytokine expression (TNFα, IL1-β, IL-6), strengthening resistance against major aquaculture pathogens including Aeromonas hydrophila, Pseudomonas aeruginosa, and Vibrio species. Challenge studies demonstrate relative percent survival improvements ranging from 48 to 73% against virulent pathogens. Haematological improvements include increased erythrocyte counts, higher haemoglobin levels at moderate inclusion rates, and beneficial effects on glucose metabolism and lipid profiles. Moringa seeds are highly effective natural water clarifiers, removing 95-99% of turbidity through cationic proteins that act as coagulants. However, their antinutritional compounds (tannins, phytates, saponins, oxalates) can harm fish at elevated levels, requiring careful dosage monitoring in aquaculture applications. This review synthesizes current evidence, identifies optimal application strategies, and provides direction for future research to maximize Moringa's potential as a sustainable, cost-effective resource for enhancing productivity, fish health, and environmental sustainability in modern aquaculture systems.</p>","PeriodicalId":23690,"journal":{"name":"Veterinary Research Communications","volume":"49 6","pages":"308"},"PeriodicalIF":2.0,"publicationDate":"2025-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12423190/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145030754","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dietary Nigella sativa oil confers protection against diazinon toxicity in nile tilapia (Oreochromis niloticus): A detoxification-based approach.","authors":"Mustafa Öz, Enes Üstüner, Merjan Jumayeva, Suat Dikel","doi":"10.1007/s11259-025-10861-2","DOIUrl":"https://doi.org/10.1007/s11259-025-10861-2","url":null,"abstract":"<p><p>This study evaluated how dietary black seed oil (Nigella sativa L.) against the diazinon waterborne toxicity on Nile tilapia (Oreochromis niloticus), focusing on growth performance, hematological and biochemical parameters as well as oxidative stress markers and histological changes. A 40-day feeding trial was carried out using four experimental groups: Group 1 (control group), Group 2 (N. sativa-supplemented group), Group 3 (diazinon-exposed group), and Group 4 (diazinon-exposed with N. sativa supplementation group). The LC₅₀ value of diazinon for Nile tilapia was determined to be 4.642 mg/L, and fish were exposed to a sublethal concentration of LC₅₀/20 during the trial. Results demonstrated that diazinon exposure significantly impaired growth, hematological parameters, and liver function while increasing oxidative stress and tissue damage. Conversely, dietary supplementation with N. sativa oil significantly mitigated these adverse effects by enhancing specific growth rate, restoring RBC, Hb, and Hct values, improving liver enzyme profiles, and increasing antioxidant enzyme activity (SOD, CAT, GPx). Histological examinations confirmed that N. sativa oil reduced diazinon-induced damage in gill and liver tissues. The results show that N. sativa oil can be used as a functional feed ingredient to enhance fish resistance to chemical stressors that are commonly found in intensive aquaculture.</p>","PeriodicalId":23690,"journal":{"name":"Veterinary Research Communications","volume":"49 5","pages":"305"},"PeriodicalIF":2.0,"publicationDate":"2025-09-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145024284","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of earwax and hair cortisol concentration in relation to environmental stressors in Hanwoo cattle.","authors":"Mohammad Ataallahi, Geun-Woo Park, Jalil Ghassemi Nejad, Kyu-Hyun Park","doi":"10.1007/s11259-025-10866-x","DOIUrl":"https://doi.org/10.1007/s11259-025-10866-x","url":null,"abstract":"<p><p>Global warming causes heat stress in livestock, impairing their health, welfare, and productivity. In bovines, chronic stress elevates cortisol levels; however, this response often goes undetected due to the lack of practical biomatrices for accurate assessment. Common biomatrices such as blood require repeated sampling that may affect measurement accuracy. This study compared earwax cortisol concentration (ECC) and hair cortisol concentration (HCC) in native Korean cattle (Hanwoo) to evaluate their usefulness in reflecting chronic environmental stress. A total of 68 earwax samples (left ear) and 68 hair samples (forehead) were collected monthly from April to July 2022 from 20 healthy cattle (mean age 30.2 ± 18.3 months). Cortisol was quantified using enzyme immunoassay kits. The temperature-humidity index (THI) reached 77 in July, indicating mild heat stress. A general linear model with Tukey's test, and Pearson correlation analysis were used to assess relationships among ECC, HCC, and THI. Average ECC and HCC were 29.02 ± 11.5 and 6.8 ± 4.9 pg/mg, respectively. ECC was higher in July than in April and June (p < 0.05) and in May than in June (p < 0.05). HCC was higher in July than in April, May, and June (p < 0.05). HCC showed differences among age groups (up to 12, 12-25, and above 25 months; p < 0.05), while ECC showed no significant age-related differences. ECC and HCC were moderately correlated (r = 0.3, p < 0.05), and both were correlated with THI (r = 0.3 and r = 0.2, respectively; p < 0.05). Overall, earwax and hair cortisol are practical biomarkers for assessing chronic heat stress, although variations in earwax production may affect the reliability of ECC.</p>","PeriodicalId":23690,"journal":{"name":"Veterinary Research Communications","volume":"49 5","pages":"306"},"PeriodicalIF":2.0,"publicationDate":"2025-09-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145024301","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Time-dependent post-mortem histomorphological changes in Penaeus vannamei following humane euthanasia.","authors":"Rodolfo Lozano-Olvera, Karla G Aguilar-Rendón, Sergio Cervantes, Selene M Abad-Rosales, Sonia A Soto-Rodriguez, María C Chávez-Sánchez","doi":"10.1007/s11259-025-10865-y","DOIUrl":"https://doi.org/10.1007/s11259-025-10865-y","url":null,"abstract":"<p><p>Histological analysis is a method commonly used in medical clinics to evaluate biological tissues and their abnormalities. However, this method is based on tissue collected from the necropsy of the animal, where modifications in the tissue associated with autolysis can occur after death. This study analyses the post-mortem histomorphological changes in white shrimp (Penaeus vannamei) after euthanasia by clove oil anaesthesia induction and pithing as a secondary killing method. The objective was to evaluate the natural progression of autolysis in specific tissues and determine the optimal time for collecting histopathological samples. Eighty clinically healthy juvenile shrimp were killed and analysed at 0, 10, 20, 40, 60, 120, 240, and 360 min post-mortem (mpd). Changes in colouration, muscle stiffness (rigor mortis), and tissue structure in the hepatopancreas, midgut, and antennal gland were examined. From 40 mpd onward, colour changes were observed in the musculature and hepatopancreas, accompanied by an increase in abdominal curvature due to the development of rigor mortis. The hepatopancreas exhibited the fastest rate of autolysis, followed by the midgut and antennal gland. Tissue integrity was lost at 10 mpd in the hepatopancreas and was severe from 60 mpd onward. A significant relationship was found between post-mortem time and the degree of tissue autolysis (r > 0.90, P < 0.001), with the hepatopancreas being the most severely affected tissue. The results indicate that post-mortem changes in P. vannamei occur rapidly, which can affect the interpretation of histopathological analyses if autolytic changes in tissues are not recognised as a post-mortem process. Immediate fixation of samples within the first 5 min after shrimp death is recommended to avoid significant alterations that could lead to misinterpretation in histological studies.</p>","PeriodicalId":23690,"journal":{"name":"Veterinary Research Communications","volume":"49 5","pages":"304"},"PeriodicalIF":2.0,"publicationDate":"2025-09-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145024276","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Engineering a recombinant VP2-Based neutralizing epitope vaccine candidate against canine parvovirus: a preliminary immunogenicity assessment.","authors":"Qing Wu, Yuhan Jin, Weiyue Cao, Zeheng Ren, Xinyu Li, Zhitong Li, Jiachi Han, Chunxu Shi, Rui Gao, Min Yan, Shengrui Zhu, Wenpeng Guan, Xinyuan Shen, Lin Bai, Guiping Ren","doi":"10.1007/s11259-025-10877-8","DOIUrl":"https://doi.org/10.1007/s11259-025-10877-8","url":null,"abstract":"<p><strong>Background: </strong>Canine parvovirus (CPV) poses a severe threat to canine health, necessitating the development of safer and more effective vaccines. While traditional vaccines carry risks of virulence reversion and environmental contamination, subunit vaccines-especially neutralizing epitope vaccines-offer promising alternatives by eliciting targeted immune responses with enhanced safety.</p><p><strong>Methods: </strong>We employed bacterial display technology to express 11 overlapping CPV VP2 gene fragments on the periplasmic membrane of E. coli. Key neutralizing antigenic epitopes of CPV VP2 were mapped using four neutralizing single-chain antibodies in combination with FCM. The identified epitopes were concatenated via GS linkers and expressed as a recombinant VP2D protein. Then, animal experiments were conducted to evaluate the immunogenicity of the epitope-based vaccine in mice.</p><p><strong>Results: </strong>The data shows that the antigenic epitopes of the four single-chain antibodies are located in V2-V3, V8, V10 and V11 respectively. After linking five antigenic epitope fragments, we constructed a novel chimeric antigen protein, VP2D, and achieved the efficient expression of VP2D. Animal immunization evaluation results demonstrated that the VP2D vaccine exhibited superior immunological properties compared to both the VP2 vaccine and the commercial vaccine. The VP2D vaccine significantly enhanced the host's capacity to mount both humoral and cellular immune responses. Notably, the antibody titers in the VP2D vaccine group were consistently 1.2- to 1.5-fold higher than those in the VP2 vaccine group throughout the experimental period. Furthermore, the proportions of CD4⁺ T cells and F4/80⁺ cells were increased by up to 12% and 8%, respectively, in the VP2D group. Additionally, both the protein levels and mRNA transcription of inflammatory cytokines were elevated in the VP2D vaccine group relative to both the VP2 and commercial vaccine groups, indicating a consistently enhanced inflammatory response.</p><p><strong>Conclusions: </strong>Identified CPV-VP2 neutralizing antigenic epitopes (70-150, 410-440, 510-585 aa), and an effective candidate vaccine for the prevention of CPV was provided.</p>","PeriodicalId":23690,"journal":{"name":"Veterinary Research Communications","volume":"49 5","pages":"298"},"PeriodicalIF":2.0,"publicationDate":"2025-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145001404","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"First molecular detection and subtyping of Blastocystis in pigs raised by hilly ethnic communities in Bangladesh: a zoonotic concern.","authors":"Jinnat Rehena, Anas Bin Harun, Abdullah Al Bayazid, Md Farhan Hasan, Tania Sultana, Ainun Nahar, Joynti Saha, Mokammel Hossain Tito, Shuvo Singha, S H M Faruk Siddiki, M Nazmul Hoque, Md Robiul Karim","doi":"10.1007/s11259-025-10864-z","DOIUrl":"https://doi.org/10.1007/s11259-025-10864-z","url":null,"abstract":"<p><p>Blastocystis is a single-celled intestinal protist found worldwide in humans and animals, including pigs, and can cause gastrointestinal disorders. It can hinder pig production and pose a potential zoonotic risk. The parasite exhibits considerable genetic diversity and is currently classified into 44 recognized subtypes (STs). Of these, nine STs have been identified in pigs, eight of which are considered zoonotic. This study aimed to investigate the molecular prevalence, genetic diversity, and zoonotic potential of Blastocystis in pigs raised by the ethnic communities in the Chattogram Hill Tracts of Bangladesh. A total of 408 fresh pig fecal samples were collected from the three hill districts, Rangamati, Khagrachhari, and Bandarban, and analyzed using polymerase chain reaction (PCR) targeting the small subunit ribosomal RNA (SSU rRNA) gene. Molecular analysis revealed an overall prevalence of Blastocystis at 20.83% (85/408). Although prevalence varied across several variables, multivariable logistic regression identified location as the only significant risk factor, with pigs from Khagrachhari being almost 18 times more likely to be infected with Blastocystis compared to those from Bandarban. Among the 85 positive samples, three STs were identified: ST1, ST3, and ST5. The predominant subtype was ST5, which accounted for 92.92% of cases. Notably, all detected STs were zoonotic, underscoring the potential risk of cross-species transmission. This study presents the first report of Blastocystis infection in domestic pigs in Bangladesh and, given its high prevalence, low genetic diversity, and zoonotic potential, underscores the need for further research on its epidemiology, genetic makeup, and transmission across the country.</p>","PeriodicalId":23690,"journal":{"name":"Veterinary Research Communications","volume":"49 5","pages":"303"},"PeriodicalIF":2.0,"publicationDate":"2025-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145001418","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}