Veterinary Research Communications最新文献

{"title":"Prevalence and genetic characterization of porcine circovirus type 2, 3 and 4 in the upper Northern region of Thailand.","authors":"Wichittra Anukool, Panuwat Yamsakul","doi":"10.1007/s11259-025-10786-w","DOIUrl":"10.1007/s11259-025-10786-w","url":null,"abstract":"<p><p>Porcine circovirus (PCV) poses challenges to swine health and production worldwide, with PCV2, PCV3, and may be PCV4 contributing to porcine circovirus-associated diseases (PCVAD). This study investigated the prevalence and genetic diversity of PCV2, PCV3, and PCV4 in fattening pigs from 42 farms in Chiang Mai, Lamphun, and Lampang Provinces, Thailand. A total of 396 blood samples were tested using real-time PCR, and positive samples were selected for partial capsid (cap) gene sequencing. Real-time PCR detected PCV2 in 22.73% of samples and PCV3 in 4.29%, with co-infection found in 2.78%. PCV4 was not detected. Although the Chi-square test (𝜒² = 0.937, P > 0.05) showed no significant association between PCV2 and PCV3 infections and the odds ratio for PCV2 and PCV3 infection was 1.92 (95% CI: 0.69-5.33, P > 0.05), and the relative risk (RR) was 1.85, but suggesting a potential link between PCV2 infection and increased PCV3 infection. Genetic analysis identified all PCV2-positive samples as PCV2d and PCV3-positive samples belonged to clades 3a and 3b, with geographical clustering. Lamphun samples contained only 3a, while both 3a and 3b were found in Lampang. These findings highlight the dominance of PCV2d and the need for targeted vaccination. While PCV2 vaccines are available, commercial vaccines for PCV3 and PCV4 remain unavailable, emphasizing the importance of ongoing surveillance and research for effective disease control in Thai swine farms.</p>","PeriodicalId":23690,"journal":{"name":"Veterinary Research Communications","volume":"49 4","pages":"218"},"PeriodicalIF":1.8,"publicationDate":"2025-06-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12152015/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144258983","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Accuracy of inspection surveillance in detecting bovine tuberculosis during slaughter in Rio Grande do Sul.","authors":"Jerônimo Miguel Vicenzi, Cristine Cerva, Fernando Sérgio Castilhos Karam, Lucas Brunelli de Moraes, Rogério Oliveira Rodrigues, Priscilla Lucas Oliveira, Angélica Cavalheiro Bertagnolli, Fabiana Quoos Mayer","doi":"10.1007/s11259-025-10787-9","DOIUrl":"https://doi.org/10.1007/s11259-025-10787-9","url":null,"abstract":"<p><p>Bovine tuberculosis (bTB) is a zoonosis whose main reservoir and source of infection is cattle. Continuous efforts for disease effective control and eradication are made worldwide, including the identification of bTB-suggestive lesions in slaughtered animals. Since granulomatous lesions could have different causes, laboratory diagnosis is important to confirm the disease etiology. The aim of this study was to evaluate the accuracy of the macroscopic bTB diagnosis during abattoir inspection compared to laboratory confirmation, and to identify macroscopic lesions characteristics associated with tuberculosis. A total of 163 tissue samples were collected in slaughterhouses from Rio Grande do Sul, South Brazil, of which 38 were classified as bTB-suggestive. Among these, 31 were confirmed as bTB-positive by laboratory diagnosis based on PCR, histopathology, and bacterial isolation, resulting in abattoir surveillance sensitivity of 93.9%, specificity of 94.7%, positive predictive value of 81.6%, negative predictive value of 98.4%, agreement of 94.51% and Kappa value of 0.84. The results indicate that abattoir surveillance in the evaluated slaughterhouses is effective in preventing bTB-positive carcasses from reaching the consumers. Significant associations of bTB diagnosis and a higher number of affected organs, pulmonary location and pasty lesion consistency were identified in univariable analyses, with the number of affected organs emerging as a significant risk factor. These characteristics can serve as useful indicators to support more targeted and efficient post-mortem inspection procedures, such as training meat inspectors to recognize and prioritize carcasses displaying them for further diagnostic testing or detailed examination.</p>","PeriodicalId":23690,"journal":{"name":"Veterinary Research Communications","volume":"49 4","pages":"215"},"PeriodicalIF":1.8,"publicationDate":"2025-06-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144235378","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The cryoprotective effects of celastrol nanoemulsion on post-thawed attributes and fertilizing ability of cryopreserved buffalo semen.","authors":"Wael A Khalil, Salwa A Elkhamy, Mohamed M Hegazy, Mahmoud A E Hassan, Sameh A Abdelnour, Mostafa A El-Harairy","doi":"10.1007/s11259-025-10781-1","DOIUrl":"10.1007/s11259-025-10781-1","url":null,"abstract":"<p><p>Celastrol (CL), a natural molecule isolated from Tripterygium wilfordii Hook. f., possesses notable therapeutic potential across a range of disease states. However, its impact on semen quality following cryopreservation has not been extensively investigated. Thus, this research comprehensively evaluated the cryoprotective effects of celastrol nanoemulsion (CNE) on buffalo semen by analyzing sperm quality, sperm kinematics, acrosome status, oxidative stress, mitochondrial membrane potential, ultrastructure, microbiota, and apoptotic-like changes. Semen samples (n = 45) were collected from five buffalo bulls and subjected to cryopreservation following a standard procedure. Prior to freezing, Tris-extenders were supplemented with celastrol nanoemulsion (CNE) at concentrations of 0 (CNE0), 25 (CNE25), 50 (CNE50), 100 (CNE100), and 200 (CNE200) µg/mL. The results showed that CNE supplementation (excluding 200 µg/mL) significantly improved sperm progressive motility, viability, and membrane integrity in post-thawed samples and after 2 h of incubation at 37 °C with 5% CO₂. Specifically, sperm kinematic parameters were significantly improved by 50 and 100 µg/mL CNE compared to other treatments (P < 0.05). Furthermore, a superior percentage of sperm with intact acrosomes was remarked in the 25, 50, and 100 µg/mL CNE groups than in the other groups (P < 0.001). Total antioxidant capacity (TAC) was significantly higher in the CNE100 group compared to the CNE0 group (P < 0.001), while no significant differences were found between CNE100 and the other treated groups (P > 0.05). Oxidative stress markers (MDA, NO, and H₂O₂) were significantly lower in CNE50 and CNE100 groups compared to all other treatments (P < 0.001). Furthermore, the CNE 100 µg/mL group exhibited a higher percentage of viable sperm and a lower percentage of apoptotic sperm than other groups (P < 0.05). The addition of CNE to the freezing extender significantly reduced the total bacterial count, spore-forming bacteria, and coliform bacteria count in post-thawed buffalo bull sperm (P < 0.001). Transmission electron microscopy analysis revealed that CNE 100 µg/mL improved and sustained sperm ultrastructure. Notably, the CNE 100 µg/mL treatment also improved the pregnancy rate compared to the control group (82.0% vs. 68.0%). Collectively, the results indicate that CNE at concentrations of 50-100 µg/mL can be effectively used for improving sperm cryo-resistance. This is achieved through improvements in sperm quality and kinematic parameters, attenuation of oxidative stress and microbiota, enhancement of mitochondrial function, preservation of sperm ultrastructure, and improved fertilizing capacity. This study highlights the potential of celastrol nanoemulsion as a nanotechnology-based strategy for optimizing assisted reproductive technologies in livestock.</p>","PeriodicalId":23690,"journal":{"name":"Veterinary Research Communications","volume":"49 4","pages":"214"},"PeriodicalIF":1.8,"publicationDate":"2025-06-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12141171/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144226822","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Prevalence of two recessive genetic disorders in Marchigiana, Romagnola and Chianina cattle breeds in the period 2022-2024.","authors":"Martina Torricelli, Carla Sebastiani, Anna Fratto, Marcella Ciullo, Fiorella Sbarra, Massimo Biagetti","doi":"10.1007/s11259-025-10780-2","DOIUrl":"https://doi.org/10.1007/s11259-025-10780-2","url":null,"abstract":"<p><p>A certain number of inherited diseases and traits in cattle have been characterized at the molecular level, identifying the causative gene variant in the DNA locus. Also the three main native Italian beef cattle breeds located in the Apennine Mountains (Chianina, Marchigiana and Romagnola) are interested by this discovery. Genetic selection in Italian beef cattle is implemented by the National Association of Italian Beef Cattle Breeders, with the aim of improving meat production, growth ability and muscle development. Contextually, bovines, particularly those with pedigree registered in the genealogical book, have to be constantly monitored for the main congenital and hereditary disorders, among them \"Paunch Calf syndrome\" and \"Congenital Ichthyosis\". Paunch Calf Syndrome (PCS) is associated with a missense variant in the KDM2B gene on chromosome 17, while Congenital Ichthyosis (CI) is caused by an insertion of a cytosine in FA2H gene causing a frame-shift on chromosome 18. The aim of this work was to present an update of the prevalence of PCS in Marchigiana and Romagnola breeds and of CI in Chianina breed, in the Central Italy territory. In the period 2022-2024, a frequency of 6.67% heterozygous PCS carriers was registered, while for CI, in 2024, a prevalence of 5.67% heterozygous carriers was observed. These findings show that cattle should be constantly monitored and the genotyping of causal variant should be used, at least for excluding risk matings of carriers to prevent further animal losses due to these two congenital disorders.</p>","PeriodicalId":23690,"journal":{"name":"Veterinary Research Communications","volume":"49 4","pages":"213"},"PeriodicalIF":1.8,"publicationDate":"2025-06-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144217102","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of high-dose alacepril on the renin-angiotensin-aldosterone system and autonomic nervous system function in healthy dogs.","authors":"Toshiharu Fukayama, Hisako Kyojima, Mao Koike, Yuki Nakamura, Asuka Tadakuma, Takuma Doi, Seijirow Goya","doi":"10.1007/s11259-025-10782-0","DOIUrl":"https://doi.org/10.1007/s11259-025-10782-0","url":null,"abstract":"<p><p>This study investigated the effects of low-dose and high-dose alacepril on the renin-angiotensin-aldosterone system (RAAS) and autonomic nervous system in healthy dogs. Six Beagle dogs received low-dose (2.0 mg/kg/day) or high-dose (6.0 mg/kg/day) alacepril for 4 weeks. Blood pressure, the urinary aldosterone-to-creatinine ratio (UAld: Cre), echocardiographic variables, and heart rate variability were assessed pre- and post-treatment. High-dose alacepril reduced blood pressure and left ventricular dimensions, indicating a reduced preload and afterload. Heart rate variability analysis showed an increased percentage of successive normal-to-normal interval differences of > 50 ms, suggesting enhanced parasympathetic activity despite detecting no changes in heart rate. UAld: Cre was not significantly different. Low-dose alacepril had no significant effect on any of these variables. In conclusion, high-dose alacepril demonstrates antihypertensive effects and enhances parasympathetic activity, which may confer cardiovascular benefits. It reduces blood pressure, decreases left ventricular preload, and increases parasympathetic activity. However, both high-dose and low-dose alacepril showed incomplete RAAS suppression. These findings highlight the need for further research to clarify its therapeutic potential in heart failure and hypertension.</p>","PeriodicalId":23690,"journal":{"name":"Veterinary Research Communications","volume":"49 4","pages":"212"},"PeriodicalIF":1.8,"publicationDate":"2025-06-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144209654","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Aspects of the reproductive biology and gonad histology of Pagellus erythrinus (Actinopterygii: perciformes: Sparidae) in the Gulf of Antalya, NE Mediterranean Sea.","authors":"Beytullah Ahmet Balci, Ramazan İkiz, Muhammed Nurullah Arslan","doi":"10.1007/s11259-025-10778-w","DOIUrl":"10.1007/s11259-025-10778-w","url":null,"abstract":"<p><p>This study examined the histological and anatomical changes in the gonads of the common pandora (Pagellus erythrinus L., 1758), a member of the Sparidae family, collected from the Gulf of Antalya (36° 49' N, 30° 38' E; 36° 46' N, 31° 18' E), NE Mediterranean Sea. A total of 273 specimens were sampled monthly from the region. The gonads were immediately dissected and fixed in 10% neutral buffered formaldehyde for 24-48 h, followed by Bouin's solution for eight hours. They were then embedded in paraffin blocks after routine histological processing. Tissue Sect. (5 μm thick) were stained using hematoxylin-eosin and periodic acid-Schiff techniques for detailed analysis. As a protogynous hermaphrodite species, the common pandora exhibited four primary stages of gonadal development: the immature stage, female maturation stages (F1-F6), transitional stages (T1-T3), and male maturation stages (M1-M5). Based on these findings, it is recommended that fishing bans be enforced from late April to early September, coinciding with the species' breeding season in the NE Mediterranean Sea.</p>","PeriodicalId":23690,"journal":{"name":"Veterinary Research Communications","volume":"49 4","pages":"211"},"PeriodicalIF":1.8,"publicationDate":"2025-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12125098/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144188138","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular docking prediction and hepatoprotective potential of genistein-enriched diet against arsenic toxicity in juvenile nile tilapia: implications for ER stress and oxidative damage.","authors":"Amany Abdel-Rahman Mohamed, Yasmina M Abd-Elhakim, Nawal Alsubaie, Mohamed M M Metwally, Tarek Khamis, Ali H El-Far, Badriyah S Alotaibi, Samah S Abuzahrah, Rowida E Ibrahim","doi":"10.1007/s11259-025-10770-4","DOIUrl":"https://doi.org/10.1007/s11259-025-10770-4","url":null,"abstract":"<p><p>The current study evaluated the effects of genistein (GEN) supplementation to alleviate the arsenic (As)-induced hepatotoxicity in Oreochromis niloticus. This was conducted in two steps: a computational prediction study (in silico) and an experimental investigation (in vivo). The prediction step involved molecular docking analysis to assess the interactions between GEN and key stress-related mRNAs in Nile tilapia. In the experimental phase, 160 Nile tilapia fingerlings were randomly assigned to four treatment groups (in four replicates/group) for 60 days: (1) a control group fed a basal diet, (2) a GEN group receiving a GEN-supplemented diet (500 mg/kg), (3) an As group exposed to 10 µg/L As, and (4) an As + GEN group, in which fish was exposed to As and fed the GEN-supplemented diet. The computational assessment of GEN's binding ability revealed strong interactions with key mRNAs associated with inflammation and misfolded protein responses. The in vivo results revealed that GEN significantly alleviated As-induced hepatic oxidative stress and hepatocellular damage by restoring liver enzyme levels, lipid profiles, and bilirubin content and restoring the serum proteins to near-normal values. Additionally, GEN downregulated the expression of endoplasmic reticulum (ER) stress- and inflammation-related genes in the liver tissue of the As + GEN group, compared to the As-exposed fish fed on a basal diet. Additionally, the histopathological analysis further confirmed that GEN supplementation mitigated hepatic tissue damage, reducing necrosis, congestion, and inflammatory cell infiltration. In conclusion, GEN supplementation effectively counteracted As-induced hepatotoxicity in Nile tilapia by modulating oxidative stress, ER stress, and inflammation while preserving liver structure and function. Also, the molecular docking results suggest that GEN interacts with the mRNAs of inflammatory and misfolded protein targets, which are increased due to exposure to As-contaminated water. All our findings highlight GEN as a promising natural dietary additive for improving hepatic health in fish inhabiting As-contaminated environments.</p>","PeriodicalId":23690,"journal":{"name":"Veterinary Research Communications","volume":"49 4","pages":"210"},"PeriodicalIF":1.8,"publicationDate":"2025-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144152033","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploring evolutionary perspectives and antibiogram analysis of biofilm-forming Staphylococcus aureus in goat mastitis.","authors":"Muhammad Umar Javed, Muhammad Ijaz, Arslan Ahmed, Hamza Rasheed, Ali Abdullah Jabir, Maria Batool, Kehkshan Shahid, Ashraf Ali, Muhammad Talha","doi":"10.1007/s11259-025-10776-y","DOIUrl":"https://doi.org/10.1007/s11259-025-10776-y","url":null,"abstract":"<p><p>Biofilm formation plays a key role in developing antimicrobial resistance in Staphylococcus aureus (S. aureus) and is thus a major virulence factor responsible for persistent intramammary infections and subclinical mastitis in goats. The current study investigated the prevalence and molecular characterization of biofilm-forming S. aureus isolated from goat mastitis, the associated risk factors, and comparative antibiogram profiling. A total of 768 milk samples were conveniently collected from farms dairy goats in three districts of Pakistan Multan, Bahawalpur, and Rahim Yar Khan. The results revealed a molecular prevalence (presence of nuc gene) of 206/426 (48.37%) for S. aureus in milk samples from goats. The risk factors analysis showed that age group, water availability, previous mastitis history, hygienic measures during milking, teat lesion, and veterinary services posed significant associations with S. aureus intramammary infections in goats. The prevalence of biofilm production by phenotypic methods i.e. Congo Red agar and Microtitre plate were recorded to be 36.90% and 27.67% respectively. In comparison, the genotypic confirmation was done by the presence of the icaA gene and was found to be 55.34%. The phylogenetic analysis of the icaA gene revealed high homology between sequences of study isolates and the isolates of other countries like India, Egypt, Japan Kenya, the USA, and China. The S. aureus subjected to the Kirby-Bauer disc diffusion method revealed the highest resistance to cefoxitin and oxytetracycline followed by gentamycin, amikacin, trimethoprim + sulphamethoxazole, and vancomycin. There was a slight variation among the resistant profile of biofilm and non-biofilm producing S. aureus strains against antimicrobial agents. Out of 206, 31.07% (64/206) tested isolates exhibited resistance to three or more antibiotics concurrently, categorizing them as multiple drug resistant (MDR) with multiple antibiotic resistance index greater than 0.2. The study concluded that biofilm-producing S. aureus is prevalent in dairy goats of different breeds, including Beetal, Teddi, Barbari, and Makhi-cheeni, across various lactation stages (early, mid, and late), regardless of milk collection times in Pakistan of Pakistan with risk to the udder health and milk quality. Moreover, the biofilm formation may be associated with multidrug resistance of study isolates.</p>","PeriodicalId":23690,"journal":{"name":"Veterinary Research Communications","volume":"49 4","pages":"209"},"PeriodicalIF":1.8,"publicationDate":"2025-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144151999","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Occurrence of mastitis pathogens in cow's milk samples from the Hanover region, north-western Germany - an overview of routine laboratory data from 2005 to 2023.","authors":"Theresa Büthe, Nadja Jessberger, Bettina Schneider, Lothar Kreienbrock, Madeleine Plötz","doi":"10.1007/s11259-025-10773-1","DOIUrl":"10.1007/s11259-025-10773-1","url":null,"abstract":"<p><p>Mastitis remains the most frequent disease in dairy cows, affecting both animal welfare and the profitability of dairy farms. In the present investigation, we retrospectively analysed 102,179 cow's milk samples, which were object of routine diagnostics at the Department of Milk Hygiene, Institute for Food Quality and Food Safety, University of Veterinary Medicine Hannover, from 2005 to 2023, regarding the occurrence of major mastitis pathogens. On average, approx. 48% of all tested samples were bacteriologically positive. Differences in the occurrence of positive findings were described regarding the season, the submitter, the reason for submission, as well as the udder quarters. Generally, the 13 most common groups of pathogens accounted for 94.0% of all positive samples. Non-aureus staphylococci and mammaliicocci (23.6%) were detected most frequently, followed by Streptococcus uberis (14.8%), Escherichia coli (13.2%), Corynebacterium spp. (9.5%), Staphylococcus aureus (6.7%), yeast (6.1%), and Enterococcus spp. (5.6%). While a slight decline was recorded for most of these pathogen groups, S. uberis and E. coli showed a significant upward trend throughout the investigation period. Moreover, a constantly high percentage of samples contaminated with more than three different colony forms was found. Monitoring long-term development of the mastitis pathogen spectrum as well as its antibiotic resistance patterns is of utmost importance to enable adaptation of hygiene and treatment strategies. This includes appropriate training of the treating veterinarians with regard to sterile milk sampling.</p>","PeriodicalId":23690,"journal":{"name":"Veterinary Research Communications","volume":"49 4","pages":"208"},"PeriodicalIF":1.8,"publicationDate":"2025-05-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12098181/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144120976","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Application of Bacillus amyloliquefaciens CECT 5940 influenced muscle satellite cells, PCNA and acute phase protein secretion in broilers.","authors":"Viera Karaffová, Renáta Szabóová, Csilla Tóthová, Rudolf Žitňan, Michaela Čechová, Martin Levkut, Erik Hudec, Zuzana Ševčíková, Monika Röntgen, Elke Albrecht, Róbert Herich","doi":"10.1007/s11259-025-10769-x","DOIUrl":"10.1007/s11259-025-10769-x","url":null,"abstract":"<p><p>Despite the growing interest in the use of probiotics in broilers as feed additives, studies conducted to investigate the effect of probiotic administration on acute phase responses and the impact on muscle growth parameters in broilers are still limited. In this study, we investigated the effect of Bacillus amyloliquefaciens CECT 5940 on relative gene expression for growth factors involved in muscle development (insulin-like growth factors, myogenic factor 5, paired-box transcription factor), percentage of proliferating antigen cell nuclei in breast muscle, and secretion of acute phase proteins (serum amyloid A, haptoglobin, alpha₁-acid glycoprotein) in the peripheral blood of broilers. Sixty one-day-old chicks from the experimental group were sprayed with a probiotic containing B. amyloliquefaciens at a dose of 1 × 10¹⁰ CFU/g directly after hatching and received the probiotic in drinking water (50 × 10¹⁰ CFU/1000 L) for 5 consecutive days of life. Sampling was performed on the 5th, 8th, and 12th day of life of the chicks. From the obtained results, we can conclude that B. amyloliquefaciens modulated the gene expression of selected growth parameters in the pectoral muscle, thereby increasing the number of satellite cells, however, their uptake into muscle fibers and thus increased hypertrophic growth was not proven at the time of the last sampling. At the same time, it demonstrated a potentiating effect on PCNA expression in chicken breast muscles in the early intensive growth phase of broiler chickens and modulated the production of acute phase proteins, which may contribute to improving adaptive processes during the growth and development of broilers.</p>","PeriodicalId":23690,"journal":{"name":"Veterinary Research Communications","volume":"49 4","pages":"207"},"PeriodicalIF":1.8,"publicationDate":"2025-05-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12095425/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144112132","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}