Veterinary Research Communications最新文献

{"title":"Physiological and growth responses of tambaqui (Colossoma macropomum) fed Croton conduplicatus essential oil and challenged with Aeromonas hydrophila.","authors":"Gilmar Amaro Pereira, Carlos Eduardo Copatti, Aline da Silva Rocha, Rafael Silva Marchão, Aline Silva de Santana, David Ramos Rocha, Mateus Matiuzzi da Costa, Jackson Roberto Guedes da Silva Almeida, Rozzanno Antônio Cavalcanti Reis de Figueiredo, Anderson Miranda de Souza, José Fernando Bibiano Melo","doi":"10.1007/s11259-024-10631-6","DOIUrl":"10.1007/s11259-024-10631-6","url":null,"abstract":"<p><p>This study aimed to assess the effect of Croton conduplicatus essential oil (CCEO) in diets for tambaqui (Colossoma macropomum) (14.50 ± 0.07 g), a native Amazonian fish. Five diets (29.25% digestible protein; 3063.14 kcal digestible energy) were prepared: 0.00 (control), 0.25, 0.50, 1.00, and 1.50 mL CCEO kg diet^-1 and evaluated for 60 days, followed by 14 additional days of bacterial infection with Aeromonas hydrophila. Fish were fed until apparent satiation four times daily and maintained in 1,000-L tanks (n = 15 fish per tank; 5 tanks per treatment; randomized design) in a recirculatory aquaculture system. The main chemical compounds of CCEO were the monoterpenes 1,8-Cineole (20.72%), p-Cymene (12.45%), and α-Phellandrene (11.46%). Animals had no mortality, even after the bacterial infection, and CCEO did not influence the number of lesions in infected fish. According to quadratic regression analysis, feeding fish diets containing 0.85 mL CCEO kg^-1 increased their growth parameters and feed intake. The feed conversion ratio was reduced with 0.25 mL CCEO kg diet^-1. Before infection, the regression showed that the 1.00 mL CCEO kg diet^-1 reduced erythrocytes and increased hemoglobin, hematimetric indices, plasma glucose, total cholesterol, albumin levels, and plasma and liver alanine aminotransferase (ALT) activities. After bacterial infection, quadratic regression analysis showed that the control group had the highest plasma glucose and albumin values, and the treatment 0.50 mL CCEO kg diet^-1 increased mean corpuscular volume values and decreased plasma ALT activity. In conclusion, 0.85 mL CCEO kg diet^-1 is recommended for tambaqui because it can potentially improve growth performance and hemato-biochemical responses; however, CCEO did not influence responses against aeromoniasis.</p>","PeriodicalId":23690,"journal":{"name":"Veterinary Research Communications","volume":"49 1","pages":"58"},"PeriodicalIF":1.8,"publicationDate":"2024-12-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142897807","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Trace elements in feathers, pectoral plumage, and blood of griffon vultures (Gyps fulvus) from a captive environment of Southern Italy.","authors":"Francesco Giuseppe Galluzzo, Gaetano Cammilleri, Pietro Riolo, Antonio Spinnato, Maria Drussilla Buscemi, Elisa Maria Domenica Messina, Licia Pantano, Gianluigi Maria Lo Dico, Andrea Macaluso, Vincenzo Ferrantelli, Francesca Di Gaudio","doi":"10.1007/s11259-024-10629-0","DOIUrl":"10.1007/s11259-024-10629-0","url":null,"abstract":"<p><p>Heavy metals and metalloids are increasingly recognised as a threat to avian health, especially in species at the top of the food chain such as vultures. Griffon Vultures (Gyps fulvus) are ideal bioindicators for studying environmental contamination due to their scavenging habits and territorial behavior. In this study, we analysed the concentrations of six trace elements (Cd, Pb, Cr, Sb, Ni, and Cu) in feathers, pectoral plumage, and blood samples of Griffon Vultures from a captive environment in Sicily, Southern Italy, using an ICP-MS method. Significant differences in the levels of trace elements were observed between different matrices (p < 0.05). The ramigal feathers were divided into rachis (basal, medial, apical), calamus, and barbs (basal, medial apical). The Principal Component Analysis (PCA) demonstrated a clear differentiation in metal accumulation between the sections of feather matrices and between pectoral plumage and blood, reflecting the diverse pathways and time of exposure and deposition. No significant differences in heavy metal concentrations were observed between sexes and age classes. This work highlights the effectiveness of using feather matrices for monitoring environmental exposure to toxic elements and highlight the importance of continuous surveillance of heavy metal contamination in reintroduced vulture populations.</p>","PeriodicalId":23690,"journal":{"name":"Veterinary Research Communications","volume":"49 1","pages":"57"},"PeriodicalIF":1.8,"publicationDate":"2024-12-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142898217","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Main pre-slaughter factors affecting carcass bruising and meat quality in beef cattle.","authors":"Ivan Vićić, Miloš Ži Petrović, Nikola Čobanović, Mirjana Dimitrijević, Nevena Grković, Milijana Sinđić, Nedjeljko Karabasil","doi":"10.1007/s11259-024-10625-4","DOIUrl":"10.1007/s11259-024-10625-4","url":null,"abstract":"<p><p>This study aimed to determine the prevalence of carcass bruising in Simmental cross-breed bulls and to characterize the bruises in terms of number, size, shape, severity, and the affected region of the carcass. In addition, the risk factors related to the pre-harvested and harvested phase of beef production and meat quality parameters, as well, were investigated. The prevalence of bruised carcasses was 40.6% (95% confidence interval, CI = 35.4-46.0%). The main risk factors related to carcass bruising were procurement of the bulls from the auction markets (odds ratio, OR = 2.94, 95% CI = 1.65-5.26) compared to the bulls that came directly from the farms and inadequate practice in the combined type of slaughterhouses (type A: OR = 6.86, 95% CI = 3.53-13.37; type B: OR = 4.76, 95% CI = 2.26-10.01) relative to the beef-only slaughterhouses (P = 0.0001). Out of the total investigated bruises, 40.7% were severe, affecting muscular parts and mainly localized on the rear parts of the carcasses. The probability of severe bruises was higher in bulls with at least one large-sized bruise than in bulls without bruises of that size (OR = 3.10, 95% CI = 1.42-6.78, P = 0.005). Concerning the risk factors, the dark cutting occurrence was significantly lower in the bull's carcasses originating from the farms and completely absent in beef-only slaughterhouses (P = 0.001). Improvement of facilities and equipment, avoiding transport through markets, and training of staff in terms of animal welfare are crucial in obtaining a concept of safe and better quality food.</p>","PeriodicalId":23690,"journal":{"name":"Veterinary Research Communications","volume":"49 1","pages":"56"},"PeriodicalIF":1.8,"publicationDate":"2024-12-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142898585","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A preliminary study on the molecular variabilities in Varroa destructor and its effect on the body measurements.","authors":"Harun Kaya Kesik, Seyma Gunyakti Kilinc, Figen Celik, Abdurrahman Gul, Sami Simsek","doi":"10.1007/s11259-024-10619-2","DOIUrl":"10.1007/s11259-024-10619-2","url":null,"abstract":"<p><p>Varroa species, commonly known as the honey bee mites, poses a significant challenge to the worldwide beekeeping industry. The aim of this study was to investigate the effect of haplotype variation on morphology of Varroa destructor. Forty-one apiaries across Bingol province (Türkiye) and its districts were visited using random sampling to examine 2440 honey bee colonies. Adult Varroa spp mites were collected from each apiary and morphometrically analysed at species level, resulting in seven mites being used to represent each apiary. Length measurements were taken of six morphometric parameters of 287 adult Varroa spp. isolates under a stereo microscope, including body length, body width, genital shield length, genital shield width, anal shield length and anal shield width. In order to analyze genetic diversity, genomic DNA was isolated from a total of 82 individual Varroa spp. isolates, and specific primers were used to amplify the mitochondrial cytochrome oxidase subunite 1 (mt-CO1) gene fragment (376 bp) by PCR followed by DNA sequence analysis. Morphometric analysis revealed that the adult Varroa spp isolates had a body width of 1691.10 ± 06.55 μm and a body length of 1125.69 ± 06.62 μm, with no significant differences between apiaries. These findings indicate that all the isolates were morphometrically identified as V. destructor. Moreover, all the sequences were highly similar to the Korean (K) haplotype of V. destructor. Six haplotypes (Hap01-Hap06) of V. destructor were identified via haplotype analysis. The haplotype groups exhibited low nucleotide diversity and were separated by 1-3 point mutations. There was no significant difference in body widths between Hap01 and other haplotypes. However, Hap03 had significantly higher body widths than Hap04 and Hap05. This indicates that haplotype differences may have an effect on morphological parameters in Varroa species.</p>","PeriodicalId":23690,"journal":{"name":"Veterinary Research Communications","volume":"49 1","pages":"55"},"PeriodicalIF":1.8,"publicationDate":"2024-12-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142883022","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular genotyping of Theileria spp. detected in horses from Corrientes City, Argentina.","authors":"Alicia P Benitez-Ibalo, Valeria N Debárbora, Atilio J Mangold, Santiago Nava, Patrick S Sebastian","doi":"10.1007/s11259-024-10618-3","DOIUrl":"10.1007/s11259-024-10618-3","url":null,"abstract":"<p><p>After the previous description and characterization of Theileria equi sensu stricto (Genotype A) in Argentina, the aim of the present study was to investigate the possible heterogeneity of 18 S rDNA genotypes circulating in this country. Therefore, blood samples from 18 horses from two different neighborhoods of Corrientes City, Corrientes Province, were analyzed for infection with Theileria species. Theileria DNA was detected in five samples (27.8%). Four nearly complete 18 S rDNA gene sequences were obtained and phylogenetic analyses were carried out. The maximum likelihood tree constructed in this study revealed that two different 18 S rDNA genotypes - namely Genotype A (T. equi sensu stricto) and Genotype C (T. equi sensu lato/Theileria haneyi) - are circulating in the same horse population. These results represent to first evidence of T. equi genotype heterogeneity in Argentina and also the first detection of a member of the T. haneyi/Genotype C group. Together with reports from Brazil, the results of this study reinforce the assumption that Genotypes A and C are the predominant genotypes in South America.</p>","PeriodicalId":23690,"journal":{"name":"Veterinary Research Communications","volume":"49 1","pages":"54"},"PeriodicalIF":1.8,"publicationDate":"2024-12-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142883023","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Metalloproteases and their inhibitors in the postpartum endometrial remodeling in dairy cows: their relationship with days to conception after parturition.","authors":"L G Grötter, S Cainelli, M B Peralta, E Angeli, E M Belotti, H H Ortega, F Rey, M M L Velázquez, N C Gareis","doi":"10.1007/s11259-024-10622-7","DOIUrl":"10.1007/s11259-024-10622-7","url":null,"abstract":"<p><p>In dairy herds, it is expected that cows will be cycling and the uterus will be ready for a new conception before the fourth week postpartum. However, an alteration in the endometrial remodeling can delay conception, increasing the parturition-to-conception interval, and consequently decreasing the reproductive performance. The endometrial matrix has a relevant participation in the processes of postpartum uterine remodeling. In this sense, the matrix metalloprotease (MMP) system and its inhibitors (TIMPs) are directly involved in the proteolytic degradation of the matrix and their action is related to the concentration of steroid hormones. The aim of this study was to evaluate the protein expression of MMP2, MMP14, MMP9, and their inhibitors, TIMP1 and TIMP2, in the luminal epithelium, glandular epithelium and stroma of endometrial biopsies from dairy cows, at 60 days in milk. Together, the results obtained provide evidence about the expression of MMP2, MMP14 and MMP9, and their inhibitors, TIMP1 and TIMP2, in the postpartum uterus of dairy cows, and about how the balance in their expression could be associated with the achievement of pregnancy. The high protein expression of MMP2, MMP14 and TIMP1 in dairy cows with short parturition-to-conception interval could be important for uterine remodeling and early conception in dairy cows. In addition, the imbalance observed in the MMP9/TIMP1 ratio could be generating an excess of gelatinase activity in the endometrium, causing a delayed conception.</p>","PeriodicalId":23690,"journal":{"name":"Veterinary Research Communications","volume":"49 1","pages":"53"},"PeriodicalIF":1.8,"publicationDate":"2024-12-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142878178","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Prevalence and genotypic diversity of Enterocytozoon bieneusi in pigeons from two parks in Zhengzhou, China.","authors":"Chunxiang Zhou, Yongqiang He, Bowen Zhang, Zhenjie Zhang, Fuchang Yu, Meng Qi","doi":"10.1007/s11259-024-10621-8","DOIUrl":"10.1007/s11259-024-10621-8","url":null,"abstract":"<p><p>Enterocytozoon bieneusi is a predominant pathogen that presents significant public health and animal husbandry challenges. Information on the prevalence and genotypes of E. bieneusi in pigeons is scarce. In this study, 207 faecal samples were collected from pigeons in two parks in Zhengzhou, China, and subjected to nested polymerase chain reaction (nested-PCR) analysis for the detection of E. bieneusi. The overall prevalence of E. bieneusi was found to be 15.5% (32/207) across the two parks. ITS sequence analysis identified only the Peru6 genotype (n = 32), which phylogenetic analysis placed within the zoonotic group 1. Among the E. bieneusi isolates, multilocus sequence typing (MLST) at MS1, MS4 and MS7 loci identified 19, 13 and 23 samples, respectively. Of the samples, 11 were successfully amplified and sequenced at all three loci, resulting in nine distinct multilocus genotypes (MLGs). This suggests that pigeons may play a role in the transmission of E. bieneusi to humans and that there is high genetic variability of E. bieneusi in pigeons from Zhengzhou in China.</p>","PeriodicalId":23690,"journal":{"name":"Veterinary Research Communications","volume":"49 1","pages":"52"},"PeriodicalIF":1.8,"publicationDate":"2024-12-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142847712","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"First molecular detection of bovine gammaherpesvirus type 4 in dairy cows of Iran and its possible role in endometritis.","authors":"Mehran Ghaemi, Mohammad Sadeghi, Mojtaba Kafi, Zohreh Pakniat","doi":"10.1007/s11259-024-10617-4","DOIUrl":"10.1007/s11259-024-10617-4","url":null,"abstract":"<p><p>A remarkable percentage of cows in industrialized dairy farms can be affected by uterine diseases including endometritis. Different viral and bacterial agents are causing endometritis in dairy cows including Trueperella pyogenes, and bovine alphaherpesvirus 1. Also, bovine gammaherpesvirus type 4 (BoGHV-4) is responsible for different reproductive disorders. The present study aimed to investigate the role of BoGHV-4 in histopathological and cytological changes of post-partum uterine diseases and its relation to bacterial co-infection. Forty-seven uteri were collected from Holstein dairy cows at least 35 days post-partum. Bacterial culture, cytological, and histopathological studies were conducted on the endometrial samples. The PCR reaction was conducted for the sequencing of the gB gene. PCR examination indicated that from 47 uteri obtained from a slaughterhouse, 13 uteri (27.7%) were positive for BoGHV-4. Statistical analysis revealed a significant relationship between virus presence, and histopathologic and cytologic endometritis (P < 0.05). Also, Escherichia coli and Fusobacterium necrophorum presence was significantly related to BoGHV-4 infection. Vast varieties of other bacterial species were isolated from different samples but the relation between these bacteria and BoGHV-4 was not significant (P > 0.05). Altogether, it can be concluded that BoGHV-4 can play some roles in establishing endometrial inflammation increasing leukocyte infiltration in endometrium, and enhancing bacterial infection in the uterus of dairy cows. This is the first study, which detected the BoGHV-4 in Iranian cows using molecular methods.</p>","PeriodicalId":23690,"journal":{"name":"Veterinary Research Communications","volume":"49 1","pages":"51"},"PeriodicalIF":1.8,"publicationDate":"2024-12-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142830026","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"In vitro simulated digestion and Caco-2 cell absorption to explore potential of soy protein isolates as whey protein substitutes in canine and feline food.","authors":"Songjun Wang, Tong Liu, Hengyan Wang, Huasong Bai, Yunliang Li, Zhanzhong Wang","doi":"10.1007/s11259-024-10616-5","DOIUrl":"10.1007/s11259-024-10616-5","url":null,"abstract":"<p><p>Digestive properties and bioavailability of soy protein isolate and whey protein in canine and feline were evaluated using in vitro simulated digestion and cell absorption experiments. The amino acids and peptides in soybean protein isolate and whey protein after digestion were detected by high-performance liquid chromatography and liquid mass spectrometry. Gastrointestinal digestion rates of soy protein isolate and whey protein were analyzed by fitting digestive kinetics curves. The degree of absorption and utilization of amino acids and peptides from the digestive products was evaluated by establishing a Caco-2 cell model. The results demonstrated that soy protein isolate, like whey protein, could produce large amounts of free amino acids and peptides distributed 100-1500 Da. The gastrointestinal digestion speed of soy protein isolate was similar with whey protein. It is worth noting from the intestinal absorption of Caco-2 cell that more than 15 amino acids produced by soy protein isolate and whey protein could be absorbed, and a large number of peptides were also utilized by Caco-2 cell. The bioavailability of soy protein isolates and whey protein for dogs respectively reached 6.30% and 9.00%, and it reached 7.40% and 16.40% in cats, respectively. Soy isolate proteins can be digested and absorbed by pets like whey proteins. These findings may provide significant strategies and support for the application of plant-based proteins in pet foods.</p>","PeriodicalId":23690,"journal":{"name":"Veterinary Research Communications","volume":"49 1","pages":"50"},"PeriodicalIF":1.8,"publicationDate":"2024-12-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142802193","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Plenty of everlasting gratitude to a minute worm!","authors":"Giovanni Di Guardo","doi":"10.1007/s11259-024-10615-6","DOIUrl":"10.1007/s11259-024-10615-6","url":null,"abstract":"","PeriodicalId":23690,"journal":{"name":"Veterinary Research Communications","volume":"49 1","pages":"49"},"PeriodicalIF":1.8,"publicationDate":"2024-12-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142802195","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}