Veterinary Research Communications最新文献

{"title":"Same parasite, diverging fates: distinct responses of tenrecs to Angiostrongylus cantonensis infection.","authors":"Anna Šipková, Petr Cibulka, Lucia Anettová, Divakaran Pandian, Jana Kačmaříková, Kristýna Javorská, Ladislav Novotný, David Modrý","doi":"10.1007/s11259-025-10870-1","DOIUrl":"10.1007/s11259-025-10870-1","url":null,"abstract":"<p><p>Tenrecs (Afrosoricida: Tenrecidae) are insectivorous mammals endemic to Madagascar, currently facing population declines due to habitat loss and subsistence hunting. Emerging infectious diseases, including parasitic infections, may pose additional threats. A comparable situation has been observed in Algerian hedgehogs (Atelerix algirus) in Mallorca, where the invasive nematode Angiostrongylus cantonensis has been associated with severe neuropathology. Given ecological parallels and the confirmed presence of A. cantonensis in Madagascar, this study aimed to assess its potential impact on tenrec health and survival. An experimental infection was conducted using Echinops telfairi, orally inoculated with 500 or 2000 third-stage larvae and monitored for 59 days through behavioral observations. Following euthanasia, artificial tissue digestion, qPCR analysis, and histopathology were performed. Baermann's larvoscopy was used to examine feces from day 39 post-infection (DPI). No neuropathological symptoms were observed. Artificial digestion revealed 11 third-stage larvae in the gastrointestinal tract of one high-dose tenrec at 36 DPI. Parasite DNA was detected in various organs of both groups; however, accumulation in brain tissue occurred only in the high-dose group, with no viable larvae visible in histological sections. E. telfairi showed no apparent sensitivity to A. cantonensis, unlike the Algerian hedgehog, which develops severe neuropathology under comparable conditions. These findings suggest that A. cantonensis exhibits host-specific outcomes, and not all mammals act as aberrant hosts facing fatal infection. The persistence of third-stage larvae up to 36 DPI raises concerns about potential transmission to predators or hypothetical human infection.</p>","PeriodicalId":23690,"journal":{"name":"Veterinary Research Communications","volume":"49 5","pages":"300"},"PeriodicalIF":2.0,"publicationDate":"2025-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12413333/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145001464","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dietary Supplementation of the Rainbow Trout, Oncorhynchus mykiss by Red Ginseng, Panax Ginseng\" Powder: Enhancing Effects on Growth, Immunity and Disease Resistance.","authors":"Junxia Liu, Chuan Jin, Hamed Ghafarifarsani","doi":"10.1007/s11259-025-10867-w","DOIUrl":"https://doi.org/10.1007/s11259-025-10867-w","url":null,"abstract":"<p><p>This study aimed to determine the effects of dietary red ginseng, Panax ginseng powder (RGP), on the growth performance, immunity, antioxidant system, and disease resistance of the rainbow trout, Oncorhynchus mykiss. Eight experimental groups were established, including a control group and seven groups fed varying levels of ginseng powder (5 to 35 g/kg) over 60 days, followed by a challenge with Streptococcus iniae. The results indicated that ginseng supplementation significantly enhanced growth parameters (P < 0.01), with the final weight (FW) of fish in the RGP₆ (fish fed 25 g/kg RGP) group reaching 56.7 g, compared to only 36.2 g in the control group (P < 0.01). Weight gain percentages (WG%), specific growth rates (SGR), and feed conversion ratios (FCR) also improved markedly in the RGP-fed groups (P < 0.01). Furthermore, Amylase, trypsin, chymotrypsin, and lipase activities increased with higher RGP levels, particularly in the RGP₇ (fish fed 30 g/kg RGP) group (P < 0.01). Immune parameters in serum, including lysozyme, complement activity (ACH₅₀), total Ig, and myeloperoxidase activity (MPO), demonstrated significant enhancements, especially in RGP₆ and RGP₇ (P < 0.01). Mucus immune responses were also improved by the dietary RGP. The highest total Ig, alkaline phosphatase, protease, and lysozyme activities in mucus were observed in the RGP₇ group (P < 0.01). The antioxidant enzyme activities, including superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPx), also showed significant increases with RGP supplementation(P < 0.01). Liver enzyme activities decreased with higher RGP levels, particularly in the RGP₇ group (P < 0.01). Malondialdehyde (MDA) levels, were significantly lower in the RGP-treated groups compared to the control (P < 0.01). Control group exhibited the highest mortality after bacterial challenge, while groups RGP₆ and RGP₇ showed significantly lower mortality rates. Overall, the findings underscore the potential of RGP as a dietary supplement to enhance growth, immunity, antioxidant activity, and disease resistance in the rainbow trout, offering promising implications for aquaculture practices.</p>","PeriodicalId":23690,"journal":{"name":"Veterinary Research Communications","volume":"49 5","pages":"299"},"PeriodicalIF":2.0,"publicationDate":"2025-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145001440","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of repeated injection of human chorionic gonadotropin on semen quality, testicular hemodynamics and hormonal profile of senile camel.","authors":"Elshymaa A Abdelnaby, Abdulrhman K Alhaider, Ibrahim A Emam","doi":"10.1007/s11259-025-10852-3","DOIUrl":"https://doi.org/10.1007/s11259-025-10852-3","url":null,"abstract":"<p><p>The current study aimed to determine the impacts of hCG injection on the reproductive performance and hormonal profile of senile camels. Ten adult senile camels were classified into two groups: control males (n = 5) and treated males (n = 5; received hCG) once/week. Testicular morphology and echotexture [testicular volume (TV), testicular echogenicity (TE), and pixel heterogeneity (PH)] were measured. Testicular blood flow (TBF), colored area (CA), testicular resistance and pulsatility indices (RI and PI), steroids [estradiol (E2) and testosterone (T), as well as nitric oxide (NO)] were estimated. Sperm motility [total motility (TM; %), non-progressive motility (NPM; %), progressive motility (PM; %)], sperm cell concentration (SCC; 10⁶ /mL), and viability (V; %) were also estimated. Examination begins one week before injection (W-1) and continued from W0 till W8 and then continued until W12 to determine post-treatment effect. Significant elevation (P < 0.05) of both TBF and CA (pixels) from W5 to W9, while TE, PH, RI, and PI were significantly declined (P < 0.05) from W4 to W8. E2, T, and NO levels were elevated (P = 0.01) from W4 to W8 for E2 and T, and from W4 to W9 for NO. Significant (P = 0.02) elevation of TM%, V%, PM%, and SCC from W8 to W11, with a decline in NPM%. T, E2, and NO positively correlated with TBF and negatively correlated with RI and PI. In conclusion, repeated administration of hCG improved the reproductive performance of senile male camels with an alteration in semen quality, steroids, testicular artery blood flow pattern, and NO levels.</p>","PeriodicalId":23690,"journal":{"name":"Veterinary Research Communications","volume":"49 5","pages":"301"},"PeriodicalIF":2.0,"publicationDate":"2025-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145001449","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ectoparasites of opossum's (Didelphimorphia: Didelphidae) captured in an urban complex in the Northern Brazilian amazon: new hosts and research on hemotropic bacteria.","authors":"Darlison Chagas-de-Souza, Claúdia Regina Silva, Ricardo Bassini-Silva, Tássio Alves-Coêlho, Lourdes Marina Bezerra Pessoa, Fernando de Castro Jacinavicius, Marcelo Bahia Labruna, Lúcio André Viana","doi":"10.1007/s11259-025-10871-0","DOIUrl":"https://doi.org/10.1007/s11259-025-10871-0","url":null,"abstract":"<p><p>Ticks and mites are important ectoparasites that affect animal and human health, directly causing harm and acting as vectors of pathogens. This study investigated the ectoparasites of synanthropic didelphids marsupials in northern Amazonia, Brazil, and screened them for hemotropic bacteria. The study was carried out in October 2022 in the metropolitan region of Macapá, Amapá State, Brazil, in vegetation remnants characterized by terra firme rainforest, alluvial forest, and savanna. Sampling was conducted over 12 consecutive days using live traps (Tomahawk and Shanon) and pitfall traps for capturing small wild mammals. The ectoparasites were removed using tweezers. A fraction of the collected ectoparasites was separated for DNA extraction and subsequent PCR amplification, and an aliquot of the extracted DNA was used for pathogen molecular tests, including bacteria and protozoa. A total of 26 larvae of Amblyomma dissimile, 17 nymphs of Amblyomma humerale, and more than 20 larvae of Eutrombicula goeldii were analyzed. Molecular identification confirmed the identity of A. dissimile and A. humerale, with new host records for Didelphis imperfecta and Didelphis marsupialis, respectively. The 16 S rRNA gene amplified successfully for 38.5% of A. dissimile larvae and all A. humerale nymphs, while the 18 S rRNA gene failed for chiggers. Pathogen screening yielded negative results. These findings contribute to the knowledge of host-parasite interactions and highlight the need for further surveillance of potential zoonotic agents in ectoparasites from wildlife.</p>","PeriodicalId":23690,"journal":{"name":"Veterinary Research Communications","volume":"49 5","pages":"302"},"PeriodicalIF":2.0,"publicationDate":"2025-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145001422","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular detection and phylogenetic analysis of lumpy skin disease virus in Yunnan Province, China from 2019 to 2023.","authors":"Jun Yao, Xue Li, Lei Tan, Lin Gao, Pei Zhu, Jiarui Xie, Rui Yan, Taoyin Yu, Fengyi Xia, Qiyun Chen, Bangquan Zeng, Jiaping Yang","doi":"10.1007/s11259-025-10879-6","DOIUrl":"10.1007/s11259-025-10879-6","url":null,"abstract":"<p><p>The lumpy skin disease (LSD), caused by the lumpy skin disease virus (LSDV), represents an emerging infectious disease that poses substantial economic losses to the cattle industries in China. This study aimed to investigate the epidemiological characteristics of LSDV in Yunnan Province, Southwest China, from 2019 to 2023. A Taqman-probe-based real-time PCR (qPCR) assay was developed for the molecular detection of LSDV nucleotides. In total, 2495 samples were collected and tested using the established method. LSDV-positive samples were further analyzed by amplifying and sequencing the GPCR and p32 genes. In addition, Viral isolation was performed to explore the biological characterisitics of the isolate. The developed qPCR assay demonstrated high sensitivity, with a limit of detection of 4.83 copies/µL. Six samples (0.24%) tested positive for LSDV, all originating from skin scabs. Molecular and phylogenetic analyses of the GPCR and p32 genes revealed that these six LSDV strains were genetically related to strains previously reported from China, Russia, and Thailand. One LSDV strain, designated YUN-LSDV, was successfully isolated in primary sheep testicular (PST) cells; while the isolate exhibited insensitivity to Madin-Darby bovine kidney cells. Collectively, this study represents the first report on the epidemiological characteristics of LSDV in cattle in Yunnan Province and contributes to the development of effective prevention and control strategies for LSD.</p>","PeriodicalId":23690,"journal":{"name":"Veterinary Research Communications","volume":"49 5","pages":"297"},"PeriodicalIF":2.0,"publicationDate":"2025-09-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144971018","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Detection of PCV2d in pig industry of the Iberian Peninsula.","authors":"Sara Gomes-Gonçalves, Sérgio Santos-Silva, Guilherme Moreira, Andreia V S Cruz, João R Mesquita","doi":"10.1007/s11259-025-10874-x","DOIUrl":"10.1007/s11259-025-10874-x","url":null,"abstract":"<p><p>Porcine circovirus type 2 (PCV2) is a major viral pathogen affecting swine worldwide, causing significant economic losses through its association with porcine circovirus-associated diseases (PCVD), which can lead to reproductive failure, growth retardation, and increased mortality in affected herds. Although vaccination against the PCV2a and PCV2b genotypes has reduced clinical disease, the PCV2d genotype is increasingly prevalent in many regions. Molecular detection was performed on fecal samples from collected from a slaughterhouse using PCR targeting the ORF2 gene to detect PCV2 genotypes. Of 400 samples tested, 8.5% (17/200; 95% CI: 5.03-13.26) of samples from Portugal were positive for PCV2d, while none from Spain tested positive. This study reports the first confirmed detection of PCV2d in domestic pigs in Portugal in indexed journals, indicating circulation within the Portuguese swine population. The absence of PCV2 in Spanish samples may reflect differences in regional epidemiology or biosecurity practices. These findings highlight the importance of ongoing molecular surveillance and farm-level monitoring to inform vaccination strategies and control measures in response to evolving PCV2 genotypes. Integrated approaches should also consider wildlife reservoirs and environmental factors to improve management of PCV2 spread in domestic swine.</p>","PeriodicalId":23690,"journal":{"name":"Veterinary Research Communications","volume":"49 5","pages":"296"},"PeriodicalIF":2.0,"publicationDate":"2025-09-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12408743/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144971328","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Invasive whistling frogs (Eleutherodactylus johnstonei) act as a reservoir for antimicrobial-resistant Enterobacteriaceae in Latin America's most populous city.","authors":"Gabrielli S Abreu, Gabriel S Dos Santos, Denise B Nogueira, Gissandra F Braz, Rafael R Soares, Nathália S Guimarães, Gisele O de Souza, Cinthia A Brasileiro, José S Ferreira Neto, Marcos B Heinemann","doi":"10.1007/s11259-025-10872-z","DOIUrl":"https://doi.org/10.1007/s11259-025-10872-z","url":null,"abstract":"<p><p>Invasive species represent a significant threat to ecological balance and the maintenance of native populations. Besides, these have been associated with the emergence of pathogens of public health importance, including multidrug-resistant bacteria. This study aimed to screen and describe the antimicrobial resistance profile of clinically important Enterobacteriaceae species isolated from whistling frogs (Eleutherodactylus johnstonei), an invasive anuran species in São Paulo, Brazil. Clinically relevant Enterobacteriaceae strains (n = 35) were isolated from oral and skin swabs of 19 whistling frogs and tested for antimicrobial susceptibility and antimicrobial resistance encoding genes. Resistance to amoxicillin + clavulanate and cefoxitin were the most frequent (16.67%; 4/24), followed by cefotaxime (5.71%; 2/35), ceftriaxone (2.86%; 1/35), and tetracycline (2.86%; 1/35). Among the antimicrobial resistance genes screened, bla_{CTX-M group 8}, bla_TEM, and bla_CMY were identified. The whole genome of the bla_{CTX-M group 8}-positive E. coli strain was assessed and confirmed bla_CTX-M-8 presence and phylogenetic analysis. Given the synanthropic behavior of whistling frogs, these amphibians may act as carriers of antimicrobial-resistant bacteria.</p>","PeriodicalId":23690,"journal":{"name":"Veterinary Research Communications","volume":"49 5","pages":"295"},"PeriodicalIF":2.0,"publicationDate":"2025-08-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144971250","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mesenchymal stem cell therapy in veterinary orthopaedics: Evidence from canine clinical medicine.","authors":"S Amitha Banu, Merlin Mamachan, Hussein M El-Husseiny, Ali Golchin, Khan Sharun","doi":"10.1007/s11259-025-10843-4","DOIUrl":"https://doi.org/10.1007/s11259-025-10843-4","url":null,"abstract":"<p><p>Mesenchymal stem cell (MSC) therapy is a promising approach for treating orthopaedic conditions in veterinary clinical patients. MSCs exhibit remarkable regenerative properties, including multilineage differentiation, immunomodulation, and tissue repair, making them an attractive alternative to conventional therapies. This comprehensive review explores the clinical potential of MSCs in managing a range of canine orthopaedic disorders, including fractures, degenerative joint diseases, and tendon injuries. Evidence from canine clinical studies highlights the ability of MSCs to enhance tissue healing, reduce pain, and restore function, offering valuable insights into their therapeutic utility. Optimizing delivery methods, such as intra-articular injections and scaffold-based approaches, is critical for maximizing therapeutic efficacy. Clinical trials in dogs have shown significant improvements in mobility and recovery, underscoring the relevance of MSC therapy for specific orthopaedic applications. Despite its potential, MSC therapy faces several challenges. One major hurdle is study heterogeneity, which complicates evidence synthesis. Differences in cell sourcing, dosage, and injection frequency make it difficult to establish a consensus on optimal treatment protocols. Future advancements should focus on expanding cell-free approaches utilizing MSC-derived bioactive molecules and extracellular vesicles. Furthermore, collaborative efforts between veterinarians, researchers, and industry partners will be critical in translating preclinical findings into viable commercial veterinary stem cell products. Large-scale, randomized clinical trials with standardized methodologies are necessary to validate the efficacy of MSC therapy across different orthopaedic conditions.</p>","PeriodicalId":23690,"journal":{"name":"Veterinary Research Communications","volume":"49 5","pages":"290"},"PeriodicalIF":2.0,"publicationDate":"2025-08-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144971041","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Advances in diagnosis of diseases causing diarrhea in newborn calves.","authors":"Doaa Sedky, Alaa A Ghazy, Hala A A Abou-Zeina","doi":"10.1007/s11259-025-10855-0","DOIUrl":"https://doi.org/10.1007/s11259-025-10855-0","url":null,"abstract":"<p><p>Diarrhea in newborn calves is a serious global health problem. It poses challenges for animal industry, veterinarians and researchers due to the rapid onset of dehydration. Mixed infections make treatment complicated, and many young calves suffer high rates of illness and death from this condition. Numerous enteropathogens are associated with diarrhea in newborn calves, encompassing viruses, bacteria, parasites, and protozoa. Their occurrence differs by region, yet the most prevalent infections include E. coli, Salmonella species, Clostridium perfringens, Clostridium difficile, Rotavirus, Coronavirus, Cryptosporidium, Toxocara, Giardia and Eimeria. This review outlines the diagnostic techniques for diseases that lead to diarrhea in newborn calves. Diagnosis is based on clinical manifestations; however, the laboratory identification of etiological items is the only valid way for detecting the illness's aetiology and initiating treatment protocols. Classic methods such as bacterial culturing, fecal flotation, direct microscopy, and virus isolation help us understand pathogens better. Immunological assays like ELISA and immunochromatography are fast, accurate, affordable, and useful for on-farm detection. They help identify specific antigens or antibodies efficiently. Molecular methods including PCR (standard, multiplex, real time and digital), LAMP assays, DNA microarrays and whole-genome sequencing allow highly accurate and sensitive detection. They can identify pathogens effectively, even at very low levels. Nanotechnology-based assays introduce a novel level of sensitivity and specificity, often yielding quick results with minimal sample volumes. In conclusion, accurate and rapid diagnosis using advanced techniques is critical for managing and preventing diseases that lead to diarrhea in newborn calves.</p>","PeriodicalId":23690,"journal":{"name":"Veterinary Research Communications","volume":"49 5","pages":"293"},"PeriodicalIF":2.0,"publicationDate":"2025-08-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12394334/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144971181","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Therapeutic application of canine adipose tissue-derived mesenchymal stromal cells in a dog with refractory immune-mediated thrombocytopenia, with diabetic ketoacidosis, and gastrointestinal bleeding induced by immunosuppressive treatment.","authors":"Hee-Won Jang, Ga-Hyun Lim, So-Young Jeung, Jae-Gon Ah, Sung-Soo Kim","doi":"10.1007/s11259-025-10849-y","DOIUrl":"https://doi.org/10.1007/s11259-025-10849-y","url":null,"abstract":"<p><p>Refractory immune-mediated thrombocytopenia (ITP) in dogs is a condition with a poor prognosis due to the poor response to immunosuppressive therapy and the adverse effects of long-term drug administration. This case report describes the successful management of refractory ITP using stem cell therapy in a dog that experienced severe side effects from immunosuppressive treatment. The patient experienced recurrent relapses when prednisolone (PDS) tapering was attempted during conventional immunosuppressive therapy, necessitating prolonged use of PDS; this led to complications, such as diabetic ketoacidosis and chronic gastrointestinal bleeding. In this case, the dog was treated with canine adipose tissue-derived mesenchymal stromal cells (cAT-MSCs) administered intravenously at four-week intervals for eight sessions. Following MSC therapy, refractory ITP was successfully treated without PDS, and the side effects associated with prolonged use of PDS and multiple immunosuppressants were resolved. Although further investigations are needed for the clinical application of cAT-derived MSCs in ITP, cAT-MSC injections could be an alternative treatment option for refractory ITP in veterinary medicine.</p>","PeriodicalId":23690,"journal":{"name":"Veterinary Research Communications","volume":"49 5","pages":"294"},"PeriodicalIF":2.0,"publicationDate":"2025-08-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144970961","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}