DNA porcine viruses detected on fresh liver samples destined for human consumption.

IF 1.8 3区农林科学 Q2 VETERINARY SCIENCES

Veterinary Research Communications Pub Date : 2025-05-02 DOI:10.1007/s11259-025-10753-5

Mariana Antunes Rezende, Yasmin Ampese Maté, Jean Francisco Maldaner Lui, Ana Clara Seibel, Rafael Frandoloso, Luiz Carlos Kreutz

{"title":"DNA porcine viruses detected on fresh liver samples destined for human consumption.","authors":"Mariana Antunes Rezende, Yasmin Ampese Maté, Jean Francisco Maldaner Lui, Ana Clara Seibel, Rafael Frandoloso, Luiz Carlos Kreutz","doi":"10.1007/s11259-025-10753-5","DOIUrl":null,"url":null,"abstract":"<p><p>Pork meat and pork-derived products are major sources of protein, but they might be important vehicles for transmitting viruses to humans. Here we aimed to evaluate whether pig enzootic viruses could be found in liver samples destined for foodstuff manufacture. We collected 140 liver samples in a slaughterhouse for DNA extraction and viral detection by real-time or conventional PCR. The samples were from 14 swine herds (n = 10/herd) located in Rio Grande do Sul state, Brazil. We found 93 (66.4%) samples positive for swine torque teno virus (TTSuV); out of these, 29 samples (31.2%) were positive for TTSuV1, 39 (41.9%) for TTSuV2 and 25 (26.9%) for both TTSuV genotypes. Porcine circovirus type 2 (PCV2) DNA was found on 24 (17.1%) samples, out of which 23 were PCV2d and only one was PCV2b. Half of the samples from each farm were tested for adenoviruses (AdV) and 44 (62.8%) were positive: by analyzing the melting curve, the samples were classified as adenoviruses of human (HAdV), canine (CAdV), and bird (AVAdV) origin in 39 (55.7%), 11 (15.7%) and 3 (4.2%) of the samples. Porcine parvovirus (PPV) DNA was detected in 5 (7.1%) of the 70 samples analyzed. Pork liver is commonly used for making pate, sausages, and mortadella which, in this situation, could harbor these viruses. Further processing of foodstuff is likely to inactive viral agents; however, our data indicates that pork-derived meat might contain viruses that eventually could reach the food chain and be harmful to immunocompromised individuals.</p>","PeriodicalId":23690,"journal":{"name":"Veterinary Research Communications","volume":"49 4","pages":"188"},"PeriodicalIF":1.8000,"publicationDate":"2025-05-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Veterinary Research Communications","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.1007/s11259-025-10753-5","RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"VETERINARY SCIENCES","Score":null,"Total":0}

引用次数: 0

Abstract

Pork meat and pork-derived products are major sources of protein, but they might be important vehicles for transmitting viruses to humans. Here we aimed to evaluate whether pig enzootic viruses could be found in liver samples destined for foodstuff manufacture. We collected 140 liver samples in a slaughterhouse for DNA extraction and viral detection by real-time or conventional PCR. The samples were from 14 swine herds (n = 10/herd) located in Rio Grande do Sul state, Brazil. We found 93 (66.4%) samples positive for swine torque teno virus (TTSuV); out of these, 29 samples (31.2%) were positive for TTSuV1, 39 (41.9%) for TTSuV2 and 25 (26.9%) for both TTSuV genotypes. Porcine circovirus type 2 (PCV2) DNA was found on 24 (17.1%) samples, out of which 23 were PCV2d and only one was PCV2b. Half of the samples from each farm were tested for adenoviruses (AdV) and 44 (62.8%) were positive: by analyzing the melting curve, the samples were classified as adenoviruses of human (HAdV), canine (CAdV), and bird (AVAdV) origin in 39 (55.7%), 11 (15.7%) and 3 (4.2%) of the samples. Porcine parvovirus (PPV) DNA was detected in 5 (7.1%) of the 70 samples analyzed. Pork liver is commonly used for making pate, sausages, and mortadella which, in this situation, could harbor these viruses. Further processing of foodstuff is likely to inactive viral agents; however, our data indicates that pork-derived meat might contain viruses that eventually could reach the food chain and be harmful to immunocompromised individuals.

查看原文本刊更多论文

在供人食用的新鲜肝脏样本中检出DNA猪病毒。

猪肉和猪肉制品是蛋白质的主要来源，但它们可能是将病毒传播给人类的重要载体。在这里，我们的目的是评估是否可以在用于食品生产的肝脏样本中发现猪地方性传染病病毒。我们在屠宰场收集了140份肝脏样本，用于DNA提取和实时或常规PCR检测病毒。样本来自巴西南大德州里约热内卢的14个猪群（n = 10/猪群）。检出猪转矩teno病毒（TTSuV）阳性93份（66.4%）；其中TTSuV1阳性29份（31.2%），TTSuV2阳性39份（41.9%），两种TTSuV基因型均阳性25份（26.9%）。在24份（17.1%）猪圆环病毒2型（PCV2）样本中检出DNA，其中23份为PCV2d， 1份为PCV2b。各养殖场半数标本进行腺病毒（AdV）检测，阳性标本44例（62.8%），经熔解曲线分析，鉴定为人（hav）、犬（CAdV）、禽（AVAdV）腺病毒39例（55.7%）、11例（15.7%）、3例（4.2%）。70份样品中有5份（7.1%）检出猪细小病毒（PPV） DNA。猪肝通常用于制作肉酱、香肠和摩泰台拉，在这种情况下，这些食物可能含有这些病毒。进一步加工的食品有可能使病毒失去活性；然而，我们的数据表明，猪肉衍生的肉类可能含有最终可能进入食物链的病毒，并对免疫功能低下的个体有害。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Veterinary Research Communications 农林科学-兽医学

CiteScore

2.50

自引率

0.00%

发文量

173

审稿时长

3 months

期刊介绍： Veterinary Research Communications publishes fully refereed research articles and topical reviews on all aspects of the veterinary sciences. Interdisciplinary articles are particularly encouraged, as are well argued reviews, even if they are somewhat controversial. The journal is an appropriate medium in which to publish new methods, newly described diseases and new pathological findings, as these are applied to animals. The material should be of international rather than local interest. As it deliberately seeks a wide coverage, Veterinary Research Communications provides its readers with a means of keeping abreast of current developments in the entire field of veterinary science.