Wellcome Open Research最新文献

{"title":"Acceptability trial of local Indonesian snack (SISTIK) enriched with chicken liver and eggshell powder as a potential food to increase micronutrient intakes among women of reproductive age.","authors":"Yenni Zuhairini, Aghnia Husnayiani Suryanto, Qorinah Estiningtyas Sakilah Adnani, Mohammad Brachim Anshari, Haidar Rizqi, Annisha Fathonah, Afini Dwi Purnamasari, Afiyah Hadiyanti Pangasih, Ayunda Jihadillah, Dina Novtyana Puspita, Dimas Erlangga Luftimas, Sofa Rahmannia, Umi Fahmida, Rosalind Gibson, Aly Diana","doi":"10.12688/wellcomeopenres.20292.2","DOIUrl":"https://doi.org/10.12688/wellcomeopenres.20292.2","url":null,"abstract":"<p><strong>Background: </strong>Chronic micronutrient deficiencies in Indonesian women and young children contribute to poor foetal and infant growth. To address this, we formulated micronutrient-enriched crackers (MEC) incorporating nutrient-rich chicken liver, rich in iron, zinc, vitamin A, and B vitamins, along with powdered eggshells as a calcium source. Known locally as ' <i>sistik</i>,' MEC may provide a sustainable solution to improve micronutrient intakes. This study evaluated MEC acceptability among non-pregnant women of reproductive age to ensure safety and feasibility before extending future research to pregnant and lactating populations.</p><p><strong>Methods: </strong>A single-blinded, two-phase acceptability trial involved 81 non-pregnant women aged 19-35 years in Ujung Berung Sub-district, Bandung City, Indonesia. In Phase 1, participants sampled both MEC and standard wheat crackers (SWC) in a test feeding session and rated each product using a 7-point cued facial response scale, evaluating colour, smell, flavour, and texture. In Phase 2, participants were randomly assigned to receive a 14-day home supply (75 g/day) of either MEC (n=41) or SWC (n=40). Adherence was determined by weighing unconsumed products. Data were analysed using mixed linear model for liking scores and a t-test for adherence.</p><p><strong>Results: </strong>MEC received favourable ratings, with no significant differences compared to SWC in liking scores for colour (MEC 4.5±1.2 vs. SWC 5.4±0.9), smell (MEC 4.9±1.5 vs. SWC 5.6±0.9), flavour (MEC 4.9±1.4 vs. SWC 5.6±0.9), and texture (MEC 5.4±1.0 vs. SWC 5.7±0.8); p-value > 0.05. Average daily consumption over 14 days was comparable between groups (MEC: 50.8±23.0 g vs. SWC: 51.0±20.0 g; mean difference: -0.2 g; 95% CI: -6.5 to 6.1; p-value = 0.802).</p><p><strong>Conclusions: </strong>MEC demonstrates reasonable acceptability and feasibility as a daily snack, supporting its potential as a dietary intervention to improve women's micronutrient intake and reduce infant stunting, especially among those women who found it appealing.</p><p><strong>Registration: </strong>ClinicalTrials.gov ( NCT04564222, 25 ^th September 2020).</p>","PeriodicalId":23677,"journal":{"name":"Wellcome Open Research","volume":"9 ","pages":"550"},"PeriodicalIF":0.0,"publicationDate":"2025-02-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11907183/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143650937","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The genome sequence of the Tropical Bluetail Damselfly, <i>Ischnura senegalensis </i>(Rambur, 1842).","authors":"Beatriz Willink","doi":"10.12688/wellcomeopenres.23747.1","DOIUrl":"https://doi.org/10.12688/wellcomeopenres.23747.1","url":null,"abstract":"<p><p>We present a genome assembly from a specimen of <i>Ischnura senegalensis</i> (Tropical Bluetail; Arthropoda; Insecta; Odonata; Coenagrionidae). The assembly contains two haplotypes with total lengths of 1,599.82 megabases and 1,602.78 megabases. Most of haplotype 1 (96.41%) is scaffolded into 14 chromosomal pseudomolecules, including the X sex chromosome, which haplotype 2 is a scaffold-level assembly. The mitochondrial genome has also been assembled and is 18.11 kilobases in length.</p>","PeriodicalId":23677,"journal":{"name":"Wellcome Open Research","volume":"10 ","pages":"104"},"PeriodicalIF":0.0,"publicationDate":"2025-02-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12033981/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144041089","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The genome sequence of an orbweaving spider, <i>Gibbaranea gibbosa</i> (Walckenaer, 1802).","authors":"Liam M Crowley, Craig S Wilding","doi":"10.12688/wellcomeopenres.23751.1","DOIUrl":"10.12688/wellcomeopenres.23751.1","url":null,"abstract":"<p><p>We present a genome assembly from a specimen of <i>Gibbaranea gibbosa</i> (orbweaving spider; Arthropoda; Arachnida; Araneae; Araneidae). The genome sequence has a total length of 2,816.88 megabases. Most of the assembly (98.61%) is scaffolded into 13 chromosomal pseudomolecules, including the X ₁ and X ₂ sex chromosomes. The mitochondrial genome has also been assembled and is 14.1 kilobases in length.</p>","PeriodicalId":23677,"journal":{"name":"Wellcome Open Research","volume":"10 ","pages":"97"},"PeriodicalIF":0.0,"publicationDate":"2025-02-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11953616/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143754755","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The genome sequence of the Coppice Mining Bee, <i>Andrena helvola</i> (Linnaeus, 1758).","authors":"Steven Falk, Joseph Monks","doi":"10.12688/wellcomeopenres.23746.1","DOIUrl":"10.12688/wellcomeopenres.23746.1","url":null,"abstract":"<p><p>We present a genome assembly from a female <i>Andrena helvola</i> (Coppice Mining Bee; Arthropoda; Insecta; Hymenoptera; Andrenidae). The genome sequence has a total length of 442.47 megabases. Most of the assembly (91.93%) is scaffolded into 7 chromosomal pseudomolecules. The mitochondrial genome has also been assembled and is 16.61 kilobases in length.</p>","PeriodicalId":23677,"journal":{"name":"Wellcome Open Research","volume":"10 ","pages":"102"},"PeriodicalIF":0.0,"publicationDate":"2025-02-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11897692/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143617277","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The genome sequence of the amphioxus, <i>Branchiostoma lanceolatum</i> (Pallas, 1774).","authors":"Patrick Adkins, John Bishop, Joanna Harley, Peter W H Holland","doi":"10.12688/wellcomeopenres.23671.1","DOIUrl":"10.12688/wellcomeopenres.23671.1","url":null,"abstract":"<p><p>We present a genome assembly from a specimen of <i>Branchiostoma lanceolatum</i> (Amphioxus; Chordata; Leptocardii; Amphioxiformes; Branchiostomatidae). The assembly contains two haplotypes with total lengths of 468.40 megabases and 465.81 megabases, respectively. Most of haplotype 1 (99.34%) is scaffolded into 19 chromosomal pseudomolecules. Haplotype 2 is a scaffold level assembly. The mitochondrial genome has also been assembled and is 15.14 kilobases in length.</p>","PeriodicalId":23677,"journal":{"name":"Wellcome Open Research","volume":"10 ","pages":"95"},"PeriodicalIF":0.0,"publicationDate":"2025-02-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11907188/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143650940","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Entomopathogenic fungi <i>Metarhizium pingshaense</i> increases susceptibility to insecticides in highly resistant malaria mosquitoes <i>Anopheles coluzzii</i>.","authors":"Doubé Lucien Lamy, Edounou Jacques Gnambani, Issiaka Sare, Souro Abel Millogo, Fatimata Aïcha Sodre, Moussa Namountougou, Mafalda Viana, Francesco Baldini, Abdoulaye Diabaté, Etienne Bilgo","doi":"10.12688/wellcomeopenres.21238.2","DOIUrl":"10.12688/wellcomeopenres.21238.2","url":null,"abstract":"<p><strong>Background: </strong><i>Metarhizium</i> spp. based mosquito control products are among the most investigated and could potentially serve as promising complements to chemical insecticides. However, limited knowledge exists on the implementation of this biocontrol tool in conjunction with primary insecticide-based strategies to achieve synergy.</p><p><strong>Methods: </strong>In laboratory bioassays, we combined 10 ⁷ conidia/ml native <i>Metarhizium pingshaense</i> strains with deltamethrin standard dose in three ways, before, after or simultaneously. These combinations were tested on laboratory insecticide resistant <i>Anopheles coluzzii</i>.</p><p><strong>Results: </strong>We found that <i>Metarhizium pingshaense</i> and deltamethrin could be combined to achieve greater mortality against a highly insecticide resistance colony of <i>Anopheles coluzzii</i>. When mosquitoes were exposed to both simultaneously, no effect was observed, as expected for an insecticide resistant colony. However, when fungi were applied earlier than deltamethrin, mosquitoes became more sensitive to insecticide with a minimum Lethal Time to kill at least 50% of mosquito population (LT50) less than 8 days. In addition, when deltamethrin exposure was followed by <i>Metarhizium</i> infection, mosquito survival was similar to <i>Metarhizium</i> alone LT50 (LT50 ~11 days).</p><p><strong>Conclusion: </strong>These findings suggest that early mosquito infection to <i>Metarhizium pingshaense</i> followed by chemical insecticide exposure synergically improve mosquito control in the laboratory.</p>","PeriodicalId":23677,"journal":{"name":"Wellcome Open Research","volume":"9 ","pages":"290"},"PeriodicalIF":0.0,"publicationDate":"2025-02-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12107690/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144162462","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The genome sequence of the European mole, <i>Talpa europaea</i> Linnaeus, 1758.","authors":"Nicola Pearce, Michelle F O'Brien, Rosa Lopez Colom","doi":"10.12688/wellcomeopenres.23759.1","DOIUrl":"10.12688/wellcomeopenres.23759.1","url":null,"abstract":"<p><p>We present a genome assembly from a female <i>Talpa europaea</i> (European mole; Chordata; Mammalia; Eulipotyphla; Talpidae). The assembly contains two haplotypes with total lengths of 2,060.98 megabases and 2,056.47 megabases. Most of haplotype 1 (98.6%) is scaffolded into 17 chromosomal pseudomolecules, including the X sex chromosome. Haplotype 2 was assembled to scaffold level. The mitochondrial genome has also been assembled and is 16.93 kilobases in length.</p>","PeriodicalId":23677,"journal":{"name":"Wellcome Open Research","volume":"10 ","pages":"98"},"PeriodicalIF":0.0,"publicationDate":"2025-02-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11909498/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143650941","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Incidence, risk and impact of ICU readmission on patient outcomes and resource utilisation in tertiary level ICUs in Nepal: A cohort study.","authors":"Diptesh Aryal, Hem Raj Paneru, Sabin Koirala, Sushil Khanal, Subhash Prasad Acharya, Arjun Karki, Dilanthi Gamaga Dona, Rashan Haniffa, Abi Beane, Jorge I F Salluh","doi":"10.12688/wellcomeopenres.18381.3","DOIUrl":"10.12688/wellcomeopenres.18381.3","url":null,"abstract":"<p><strong>Background: </strong>Readmissions to Intensive Care Units (ICUs) result in increased morbidity, mortality, and ICU resource utilisation (e.g. prolonged mechanical ventilation), and as such, is a widely utilised metric of quality of critical care. Most of the evidence on incidence, characteristics, associated risk factors and attributable outcomes of readmission to ICU are from studies performed in high-income countries This study explores the determinants of risk attributable to ICU readmission in four ICUs in Kathmandu, Nepal.</p><p><strong>Methods: </strong>The registry reported data on case mix, severity of illness, in-ICU interventions (including organ support), ICU outcome, and readmission characteristics. Data were captured in all adult patients admitted between September 2019 and February 2021. Population and ICU encounter characteristics were compared between those with and without readmission. Independent risk factors for readmission were assessed using univariate analysis.</p><p><strong>Results: </strong>In total 2955 patients were included in the study. Absolute ICU readmission rate was 5.69 % (n=168) for all four ICUs. Median time from ICU discharge to readmission was 3 days (IQR=8,1). Of those readmitted, 29.17% (n=49) were discharged at night following their index admission. ICU mortality was higher following readmission to ICU(p=0.016) and mortality was increased further in patients whose primary index discharge was at night(p= 0.019). Primary diagnosis, age, and use of organ support in the first 24hrs of index admission were all independently attributable risk factors for readmission.</p><p><strong>Conclusions: </strong>ICU readmission rates were adversely associated with significantly poorer outcomes, increased ICU resource utilisation. Clinical and organisational characteristics influenced risk of readmission and outcome.</p>","PeriodicalId":23677,"journal":{"name":"Wellcome Open Research","volume":"7 ","pages":"272"},"PeriodicalIF":0.0,"publicationDate":"2025-02-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11959250/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143765249","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The genome sequence of the Common Sheetweb Spider <i>Linyphia triangularis</i> (Clerck, 1757).","authors":"Olga Sivell, Duncan Sivell","doi":"10.12688/wellcomeopenres.23754.1","DOIUrl":"10.12688/wellcomeopenres.23754.1","url":null,"abstract":"<p><p>We present a genome assembly from a male <i>Linyphia triangularis</i> (Common Sheetweb Spider Arthropoda; Arachnida; Araneae; Linyphiidae). The genome sequence has a total length of 1,349.10 megabases. Most of the assembly (95.36%) is scaffolded into 13 chromosomal pseudomolecules, including the X ₁ and X ₂ sex chromosomes. The mitochondrial genome has also been assembled and is 15.31 kilobases in length.</p>","PeriodicalId":23677,"journal":{"name":"Wellcome Open Research","volume":"10 ","pages":"92"},"PeriodicalIF":0.0,"publicationDate":"2025-02-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11904403/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143626054","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dietary and related data collected during pregnancy in the Avon Longitudinal Study of Parents and Children (ALSPAC).","authors":"Caroline M Taylor, Kate Northstone, Jean Golding, Louise Jones, Genevieve Buckland, Pauline M Emmett","doi":"10.12688/wellcomeopenres.23464.2","DOIUrl":"10.12688/wellcomeopenres.23464.2","url":null,"abstract":"<p><p>The Avon Longitudinal Study of Parents and Children (ALSPAC) is a longitudinal birth cohort study based in the south-west of the UK. Pregnant women resident in and around the city of Bristol with expected delivery dates between 1 April 1991 and 31 December 1992 were invited to take part. The initial number of pregnancies enrolled was 14,541. Four questionnaires were sent to women through the post during pregnancy (including a food frequency questionnaire (FFQ) at 32 weeks) for self-completion; excluding withdrawals and exclusions, 11,978 FFQ records are available (April 2024). The main part of the FFQ at 32 weeks comprised questions on the weekly frequency of consumption of 43 food groups and food items. More detailed questions covered a further eight foods/drinks normally consumed daily. The data were used to derive daily nutrient intakes for each participant. Dietary patterns were derived using principal components analysis. Together with the diverse ALSPAC resource containing detailed data on demographics, lifestyle, environment, genetics and health, these data form a unique resource for the study of: (1) maternal diet in pregnancy, fetal/child development and their life course; (2) women's diet and their life course.</p>","PeriodicalId":23677,"journal":{"name":"Wellcome Open Research","volume":"10 ","pages":"6"},"PeriodicalIF":0.0,"publicationDate":"2025-02-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11904399/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143625971","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}