Wellcome Open Research最新文献

{"title":"The genome sequence of an ichneumonid wasp, <i>Polytribax perspicillator</i> (Gravenhorst, 1807).","authors":"Gavin R Broad, Liam M Crowley, Inez Januszczak, Chris Fletcher, Ian Barnes, James McCulloch, Augustijn De Ketelaere","doi":"10.12688/wellcomeopenres.23769.1","DOIUrl":"https://doi.org/10.12688/wellcomeopenres.23769.1","url":null,"abstract":"<p><p>We present a genome assembly from a male <i>Polytribax perspicillator</i> (ichneumonid wasp; Arthropoda; Insecta; Hymenoptera; Ichneumonidae). The genome sequence has a total length of 314.21 megabases. Most of the assembly (99.94%) is scaffolded into 8 chromosomal pseudomolecules. The mitochondrial genome has also been assembled and is 25.68 kilobases in length.</p>","PeriodicalId":23677,"journal":{"name":"Wellcome Open Research","volume":"10 ","pages":"140"},"PeriodicalIF":0.0,"publicationDate":"2025-03-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12015431/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143985075","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The genome sequence of the poplar hawk-moth, <i>Laothoe populi</i>  (Linnaeus, 1758).","authors":"Douglas Boyes, Peter W H Holland","doi":"10.12688/wellcomeopenres.17191.2","DOIUrl":"10.12688/wellcomeopenres.17191.2","url":null,"abstract":"<p><p>We present a genome assembly from an individual female <i>Laothoe populi</i> (the poplar hawk-moth; Arthropoda; Insecta; Lepidoptera; Sphingidae). The genome sequence is 576 megabases in span. Most of the assembly is scaffolded into 29 chromosomal pseudomolecules, with the W and Z sex chromosome assembled.</p>","PeriodicalId":23677,"journal":{"name":"Wellcome Open Research","volume":"6 ","pages":"237"},"PeriodicalIF":0.0,"publicationDate":"2025-03-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11937781/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143721598","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The genome sequence of a bark-dwelling beetle, <i>Silvanus unidentatus</i> (Olivier, 1790).","authors":"Maxwell V L Barclay, Toby Turner, Mark G Telfer, Michael F Geiser","doi":"10.12688/wellcomeopenres.23771.1","DOIUrl":"https://doi.org/10.12688/wellcomeopenres.23771.1","url":null,"abstract":"<p><p>We present a genome assembly from a specimen of <i>Silvanus unidentatus</i> (bark-dwelling beetle; Arthropoda; Insecta; Coleoptera; Silvanidae). The genome sequence has a total length of 187.15 megabases. Most of the assembly (84.24%) is scaffolded into 7 chromosomal pseudomolecules, including the X sex chromosome. The mitochondrial genome has also been assembled and is 17.15 kilobases in length. Gene annotation of this assembly on Ensembl identified 11,364 protein-coding genes.</p>","PeriodicalId":23677,"journal":{"name":"Wellcome Open Research","volume":"10 ","pages":"136"},"PeriodicalIF":0.0,"publicationDate":"2025-03-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11979581/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144052704","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sleep duration, sleep disturbances and skeletal muscle mass change over time: A population-based longitudinal analysis in Peru.","authors":"Renzo A Agurto-García, Enrique S Nuñez-Del-Arco, Rodrigo M Carrillo-Larco, J Jaime Miranda, Antonio Bernabe-Ortiz","doi":"10.12688/wellcomeopenres.23077.3","DOIUrl":"10.12688/wellcomeopenres.23077.3","url":null,"abstract":"<p><strong>Background: </strong>The skeletal muscle has mainly a structural function and plays a role in human's metabolism. Besides, the association between sleep quality and muscle mass, in the form of sarcopenia, has been reported. This study aimed to assess whether changes of skeletal muscle mass (SMM) over time are associated with baseline sleep duration and disturbances in a resource-constrained adult Peruvian population.</p><p><strong>Materials and methods: </strong>Secondary analysis using information of a population-based intervention. The outcome was SMM assessed using bioimpedance and the second version of the Lee's formula. The exposures were baseline self-reported sleep duration (normal, short and long sleepers) and disturbances (sleep difficulties and awakening at nights). Crude and adjusted linear mixed models were used to assess the associations of interest, and coefficients (β) and 95% confidence intervales (95% CI) were reported.</p><p><strong>Results: </strong>Data from 2,310 individuals at baseline, mean age 43.4 (SD: 17.2), and 1,163 (50.4%) females were analyzed. Sleep duration was 7.8 (SD: 1.3) hours/day, with 15.3% short sleepers and 11.6% long sleepers, whereas 24.2% reported sleep difficulties and 25.1% awakening at nights. In multivariable model, SMM among short and long sleepers did not vary significantly over time using the Lee's formula; however, SMM was lower at the end of follow-up for long sleepers using bioimpedance (-0.26 kg; 95% CI: -0.47 to -0.06). Sleep disturbances were associated with a gradual SMM reduction: 0.36 kg using bioimpedance and 0.25 kg using the formula at the end of follow-up.</p><p><strong>Conclusions: </strong>Using bioimpedance and formula estimations, sleep disturbances were associated with a reduction of SMM over a period of 2.4 years. Regarding sleep duration, no SMM changes over time were seen in short sleepers, but findings were discordant in long sleepers: a reduction of SMM using bioimpedance, but no change using the formula.</p>","PeriodicalId":23677,"journal":{"name":"Wellcome Open Research","volume":"9 ","pages":"565"},"PeriodicalIF":0.0,"publicationDate":"2025-03-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11757916/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143047750","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The genome sequence of the True Lover's Knot moth, <i>Lycophotia porphyrea</i> (Denis & Schiffermüller), 1775.","authors":"Jonathan Davis, Andy Griffiths","doi":"10.12688/wellcomeopenres.22897.2","DOIUrl":"10.12688/wellcomeopenres.22897.2","url":null,"abstract":"<p><p>We present a genome assembly from an individual male <i>Lycophotia porphyrea</i> (the True Lover's Knot; Arthropoda; Insecta; Lepidoptera; Noctuidae). The genome sequence has a total length of 542.40 megabases. Most of the assembly is scaffolded into 31 chromosomal pseudomolecules, including the Z sex chromosome. The mitochondrial genome has also been assembled and is 15.39 kilobases in length. Gene annotation of this assembly at Ensembl identified 17,907 protein-coding genes.</p>","PeriodicalId":23677,"journal":{"name":"Wellcome Open Research","volume":"9 ","pages":"469"},"PeriodicalIF":0.0,"publicationDate":"2025-03-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11923537/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143671080","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The genome sequence of the Maple Pug moth, <i>Eupithecia inturbata</i> (Hübner, 1817).","authors":"Finley Hutchinson, Liam M Crowley","doi":"10.12688/wellcomeopenres.23796.1","DOIUrl":"https://doi.org/10.12688/wellcomeopenres.23796.1","url":null,"abstract":"<p><p>We present a genome assembly from a female <i>Eupithecia inturbata</i> (Maple Pug; Arthropoda; Insecta; Lepidoptera; Geometridae). The genome sequence has a total length of 427.76 megabases. Most of the assembly (99.94%) is scaffolded into 31 chromosomal pseudomolecules, including the Z sex chromosome. The mitochondrial genome has also been assembled, with a length of 15.33 kilobases. Gene annotation of this assembly on Ensembl identified 12,386 protein-coding genes.</p>","PeriodicalId":23677,"journal":{"name":"Wellcome Open Research","volume":"10 ","pages":"141"},"PeriodicalIF":0.0,"publicationDate":"2025-03-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11992513/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144026266","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The genome sequence of a chironomid fly, <i>Chironomus tentans</i> Fabricius, 1805.","authors":"Liam M Crowley","doi":"10.12688/wellcomeopenres.23907.1","DOIUrl":"https://doi.org/10.12688/wellcomeopenres.23907.1","url":null,"abstract":"<p><p>We present a genome assembly from a specimen of <i>Chironomus tentans</i> (chironomid fly; Arthropoda; Insecta; Diptera; Chironomidae). The genome sequence has a total length of 185.47 megabases. Most of the assembly (99.7%) is scaffolded into 4 chromosomal pseudomolecules. The mitochondrial genome has also been assembled, with a length of 16.09 kilobases. Gene annotation of this assembly on Ensembl identified 12,575 protein-coding genes.</p>","PeriodicalId":23677,"journal":{"name":"Wellcome Open Research","volume":"10 ","pages":"133"},"PeriodicalIF":0.0,"publicationDate":"2025-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12038343/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144039822","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The genome sequence of the greater argentine, <i>Argentina silus</i> (Ascanius, 1775).","authors":"Helga Bára Mohr Vang, Ian Salter, Sunnvør Í Kongsstovu, Svein-Ole Mikalsen","doi":"10.12688/wellcomeopenres.23691.1","DOIUrl":"10.12688/wellcomeopenres.23691.1","url":null,"abstract":"<p><p>We present a genome assembly from a male specimen of <i>Argentina silus</i> (the greater argentine; Chordata; Actinopteri; Argentiniformes; Argentinidae). The genome sequence has a total length of 670.70 megabases. Most of the assembly is scaffolded into 24 chromosomal pseudomolecules, including the X and Y sex chromosomes. The mitochondrial genome has also been assembled and is 16.64 kilobases in length. Gene annotation of this assembly on Ensembl identified 19,422 protein-coding genes.</p>","PeriodicalId":23677,"journal":{"name":"Wellcome Open Research","volume":"10 ","pages":"131"},"PeriodicalIF":0.0,"publicationDate":"2025-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12198749/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144508596","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of a six-minute walk test in the DE50-MD canine model of Duchenne muscular dystrophy and its effect on blood-borne biomarkers.","authors":"Dominique Riddell, Rachel Harron, John Hildyard, Dominic Wells, Richard Piercy","doi":"10.12688/wellcomeopenres.23269.2","DOIUrl":"https://doi.org/10.12688/wellcomeopenres.23269.2","url":null,"abstract":"<p><strong>Background: </strong>Duchenne muscular dystrophy (DMD) is a fatal muscle wasting disease caused by mutations in the dystrophin gene resulting in cycles of muscle degeneration, inflammation and regeneration. The 6-minute walk test (6MWT) is a key functional outcome measure for DMD patient clinical trials and has been adapted for use in animal models of the disease. The DE50-MD dog model of DMD closely reflects the DMD patient phenotype prior to loss of ambulation. For pre-clinical trials using this model, functional outcome measures must be established.</p><p><strong>Methods: </strong>This longitudinal study compared distance walked in a 6MWT by DE50-MD and WT control dogs and assessed the utility of the 6MWT as a functional biomarker. Dogs underwent two 6MWTs conducted approximately 48-hours apart, at 3, 6, 9, 12, 15 and 18 months of age. In addition, we evaluated the stability of selected blood-borne biomarkers in 12-month old DE50-MD and WT dogs 0, 3, 6, 24 and 48 hours following a 6MWT.</p><p><strong>Results: </strong>DE50-MD dogs exhibited significantly shorter 6-minute walk distance (6MWD) than WT dogs at all timepoints (P<0.05), with no difference in 6MWD between the first and second 6MWT. C-C motif chemokine ligand 2 (CCL2), myomesin-3 (MYOM3) and myostatin (MSTN) were biomarkers of the DE50-MD phenotype that remained unchanged in DE50-MD dogs following the 6MWT, while creatine kinase (CK) activity significantly increased 3-hours following the test in DE50-MD dogs but remained unchanged in WT dogs.</p><p><strong>Conclusions: </strong>The 6MWT effectively discriminates DE50-MD from WT dogs aged 3-18 months and a single 6MWT is sufficient for future studies. Serum MYOM3, CCL2 and MSTN are good biomarkers of the DE50-MD phenotype that are unaffected by the relatively low level exertion performed in the 6MWT by 12-month-old DE50-MD dogs.</p>","PeriodicalId":23677,"journal":{"name":"Wellcome Open Research","volume":"9 ","pages":"681"},"PeriodicalIF":0.0,"publicationDate":"2025-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12022548/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144050639","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The genome sequence of the Yellow-legged Gull, <i>Larus michahellis</i> Naumann, 1840.","authors":"Elisa Ramos, Manuel Schweizer, Meng Yue Wu, Christophe Sahli, Constantin Latt, Maurice Lunak, Pierre-André Crochet, Walter Salzburger, Joana Meier, David Alexander Marques","doi":"10.12688/wellcomeopenres.23849.1","DOIUrl":"10.12688/wellcomeopenres.23849.1","url":null,"abstract":"<p><p>We present a genome assembly from a female specimen of <i>Larus michahellis</i> (Yellow-legged Gull; Chordata; Aves; Charadriiformes; Laridae). The genome sequence has a total length of 1,405.56 megabases. Most of the assembly (90.55%) is scaffolded into 35 chromosomal pseudomolecules, including the W and Z sex chromosomes. The mitochondrial genome has also been assembled and is 16.79 kilobases in length.</p>","PeriodicalId":23677,"journal":{"name":"Wellcome Open Research","volume":"10 ","pages":"129"},"PeriodicalIF":0.0,"publicationDate":"2025-03-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12082078/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144095336","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}