Wellcome Open Research最新文献

{"title":"Migration policies versus public health - the ethics of Covid-19 related movement restrictions for asylum seekers in reception centers in Greece in 2020.","authors":"George Makris","doi":"10.12688/wellcomeopenres.20547.2","DOIUrl":"10.12688/wellcomeopenres.20547.2","url":null,"abstract":"<p><strong>Background: </strong>The emergency context of the Covid-19 pandemic necessitated the use of national and international public health measures of unprecedented scale to minimize mortality and morbidity, often in conflict with other principles and rights, such as the autonomy of individuals. Concerns have been voiced that for populations facing precarity, such as migrants, a disproportionate and unfair application of restrictive measures, deficient application of protective measures, and even enforcement of restrictive migration policies under the pretext of the pandemic has occurred.</p><p><strong>Methods: </strong>Experts have proposed various principles as possible moral foundations of public health interventions. The author used two public health ethics frameworks to examine the ethical acceptability of movement restrictions on asylum seekers residing in refugee camps in Greece from March 2020 to October 2020.</p><p><strong>Results: </strong>Most of the principles described in the frameworks for the ethical application of movement restrictions were not adhered to. Main concerns include that, measures were prolonged despite lack of evidence about their effectiveness to reduce morbidity and mortality, while posing severe and disproportionate burdens for this population.</p><p><strong>Conclusions: </strong>An ethically acceptable public health response to Covid-19 is incompatible with certain living conditions of refugees, asylum seekers, and migrants. Moral and political determinants of health, such as social inequalities and criteria for health resources allocation, can shape the form and effectiveness of public health interventions during emergencies. The role of the discipline of public health to address these underlying determinants, that influence health-related outcomes, is an important moral question in itself. It is essential for public health professionals to be aware of the moral theorizations that underpin their work, so as to ensure that their policies align with them and to contribute to the debate that shapes these determinants.</p>","PeriodicalId":23677,"journal":{"name":"Wellcome Open Research","volume":"9 ","pages":"115"},"PeriodicalIF":0.0,"publicationDate":"2024-12-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11409434/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142296675","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Awake prone positioning effectiveness in moderate to severe COVID-19 a randomized controlled trial.","authors":"Nguyen Thanh Phong, Du Hong Duc, Ho Bich Hai, Nguyen Thanh Nguyen, Le Dinh Van Khoa, Le Thuy Thuy Khanh, Luu Hoai Bao Tran, Nguyen Thi My Linh, Cao Thi Cam Van, Dang Phuong Thao, Nguyen Thi Diem Trinh, Pham Tieu Kieu, Nguyen Thanh Truong, Vo Tan Hoang, Nguyen Thanh Ngoc, Tran Thi Dong Vien, Vo Trieu Ly, Tran Dang Khoa, Abigail Beane, James Anibal, Guy E Thwaites, Ronald Geskus, David Clifton, Nguyen Thi Phuong Dung, Evelyne Kestelyn, Guy Glover, Le Van Tan, Lam Minh Yen, Nguyen Le Nhu Tung, Nguyen Thanh Dung, C Louise Thwaites","doi":"10.12688/wellcomeopenres.22792.2","DOIUrl":"10.12688/wellcomeopenres.22792.2","url":null,"abstract":"<p><strong>Background: </strong>Awake prone positioning (APP) may be beneficial in patients with respiratory failure who are not receiving mechanical ventilation. Randomized controlled trials of APP have been performed during peak COVID-19 periods in unvaccinated populations, with limited data on compliance or patient acceptability. We aimed to evaluate the efficacy and acceptability of APP in a lower-middle income country in an open-label randomized controlled trial using a dedicated APP implementation team and wearable continuous-monitoring devices.</p><p><strong>Methods: </strong>The trial was performed at a tertiary level hospital in Ho Chi Minh City, Vietnam, recruiting adults (≥18 years) hospitalized with moderate or severe COVID-19 and receiving supplemental oxygen therapy via nasal/facemask systems or high-flow nasal cannula (HFNC). Patients were allocated by a computer-generated random number sequence in a 1:1 ratio to standard care or APP, where a dedicated team provided bedside support. Wearable devices continuously recorded pulse oximetry and body position continuously. Our primary outcome was escalation of respiratory support within 28 days of randomization.</p><p><strong>Results: </strong>Ninety-three patients were enrolled in this study between March 2022 and March 2023. Eighty (86%) patients had received ≥2 doses of SARS-CoV2 vaccine. The study was terminated early because of a reduction in the number of eligible patients. Data from 46 patients allocated to APP and 47 to standard care were available for analysis. At baseline, 19/47 (40%) patients allocated to the standard care group and 14/46 (30%) patients allocated to the APP group received HFNC. Continuous monitoring data were available for all patients monitored with wearable devices. Significantly greater mean daily APP times were achieved in those allocated to APP, however, most achieved less than the target 8 h/day. We did not detect clear differences in the primary outcome (relative risk,RR, 0.85, 95% CI 0.40-1.78, p=0.67) or secondary outcomes, including intubation rate and 28-day mortality. Patients reported prone positioning was comfortable, although almost all patients preferred supine positioning. No adverse events associated with the intervention were observed.</p><p><strong>Conclusions: </strong>APP was not associated with benefit, but there was no sign of harm. Continuous monitoring with wearable devices is both feasible and acceptable for patients. In our population, achieving prolonged APP time was challenging despite a dedicated support team, and patients preferred supine positioning.</p><p><strong>Clinical trials registration: </strong>NCT05083130.</p>","PeriodicalId":23677,"journal":{"name":"Wellcome Open Research","volume":"9 ","pages":"543"},"PeriodicalIF":0.0,"publicationDate":"2024-12-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11626102/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142801577","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Daily life in the Open Biologist's second job, as a Data Curator.","authors":"Livia C T Scorza, Tomasz Zieliński, Irina Kalita, Alessia Lepore, Meriem El Karoui, Andrew J Millar","doi":"10.12688/wellcomeopenres.22899.1","DOIUrl":"10.12688/wellcomeopenres.22899.1","url":null,"abstract":"<p><strong>Background: </strong>Data reusability is the driving force of the research data life cycle. However, implementing strategies to generate reusable data from the data creation to the sharing stages is still a significant challenge. Even when datasets supporting a study are publicly shared, the outputs are often incomplete and/or not reusable. The FAIR (Findable, Accessible, Interoperable, Reusable) principles were published as a general guidance to promote data reusability in research, but the practical implementation of FAIR principles in research groups is still falling behind. In biology, the lack of standard practices for a large diversity of data types, data storage and preservation issues, and the lack of familiarity among researchers are some of the main impeding factors to achieve FAIR data. Past literature describes biological curation from the perspective of data resources that aggregate data, often from publications.</p><p><strong>Methods: </strong>Our team works alongside data-generating, experimental researchers so our perspective aligns with publication authors rather than aggregators. We detail the processes for organizing datasets for publication, showcasing practical examples from data curation to data sharing. We also recommend strategies, tools and web resources to maximize data reusability, while maintaining research productivity.</p><p><strong>Conclusion: </strong>We propose a simple approach to address research data management challenges for experimentalists, designed to promote FAIR data sharing. This strategy not only simplifies data management, but also enhances data visibility, recognition and impact, ultimately benefiting the entire scientific community.</p>","PeriodicalId":23677,"journal":{"name":"Wellcome Open Research","volume":"9 ","pages":"523"},"PeriodicalIF":0.0,"publicationDate":"2024-12-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11445645/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142366724","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Kinetics of naturally induced binding and neutralising anti-SARS-CoV-2 antibody levels and potencies among SARS-CoV-2 infected Kenyans with diverse grades of COVID-19 severity: an observational study.","authors":"John Kimotho, Yiakon Sein, Shahin Sayed, Reena Shah, Kennedy Mwai, Mansoor Saleh, Perpetual Wanjiku, Jedidah Mwacharo, James Nyagwange, Henry Karanja, Bernadette Kutima, John N Gitonga, Daisy Mugo, Ann Karanu, Linda Moranga, Viviane Oluoch, Jasmit Shah, Julius Mutiso, Alfred Mburu, Zaitun Nneka, Peter Betti, Wanzila Usyu Mutinda, Abdirahman Issak Abdi, Philip Bejon, Lynette Isabella Ochola-Oyier, George M Warimwe, Eunice W Nduati, Francis M Ndungu","doi":"10.12688/wellcomeopenres.19414.2","DOIUrl":"10.12688/wellcomeopenres.19414.2","url":null,"abstract":"<p><strong>Background: </strong>Given the low levels of coronavirus disease 2019 (COVID-19) vaccine coverage in sub-Saharan Africa (sSA), despite high levels of natural severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) exposures, strategies for extending the breadth and longevity of naturally acquired immunity are warranted. Designing such strategies will require a good understanding of naturally acquired immunity.</p><p><strong>Methods: </strong>We measured whole-spike immunoglobulin G (IgG) and spike-receptor binding domain (RBD) total immunoglobulins (Igs) on 585 plasma samples collected longitudinally over five successive time points within six months of COVID-19 diagnosis in 309 COVID-19 patients. We measured antibody-neutralising potency against the wild-type (Wuhan) SARS-CoV-2 pseudovirus in a subset of 51 patients over three successive time points. Binding and neutralising antibody levels and potencies were then tested for correlations with COVID-19 severities.</p><p><strong>Results: </strong>Rates of seroconversion increased from day 0 (day of PCR testing) to day 180 (six months) (63.6% to 100 %) and (69.3 % to 97%) for anti-spike-IgG and anti-spike-RBD binding Igs, respectively. Levels of these binding antibodies peaked at day 28 (p<0.01) and were subsequently maintained for six months without significant decay (p>0.99). Similarly, antibody-neutralising potencies peaked at day 28 (p<0.01) but declined by three-fold, six months after COVID-19 diagnosis (p<0.01). Binding antibody levels were highly correlated with neutralising antibody potencies at all the time points analysed (r>0.60, p<0.01). Levels and potencies of binding and neutralising antibodies increased with disease severity.</p><p><strong>Conclusions: </strong>Most COVID-19 patients generated SARS-CoV-2 specific binding antibodies that remained stable in the first six months of infection. However, the respective neutralising antibodies decayed three-fold by month-six of COVID-19 diagnosis suggesting that they are short-lived, consistent with what has been observed elsewhere in the world. Thus, regular vaccination boosters are required to sustain the high levels of anti-SARS-CoV-2 naturally acquired neutralising antibody potencies in our population.</p>","PeriodicalId":23677,"journal":{"name":"Wellcome Open Research","volume":"8 ","pages":"350"},"PeriodicalIF":0.0,"publicationDate":"2024-12-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11617823/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142786923","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Case Report: Soft tissue infection with <i>Burkholderia thailandensis</i> capsular variant: case report from the Lao PDR.","authors":"Souphaphone Vannachone, Manophab Luangraj, David Dance, Narisara Chantratita, Natnaree Saiprom, Rathanin Seng, Sarunporn Tandhavanant, Sayaphet Rattanavong, Andrew Simpson, Tamalee Roberts","doi":"10.12688/wellcomeopenres.22706.1","DOIUrl":"10.12688/wellcomeopenres.22706.1","url":null,"abstract":"<p><strong>Background: </strong><i>Burkholderia thailandensis</i> is an environmental bacteria closely related to <i>Burkholderia pseudomallei</i> that rarely causes infection in humans. Some environmental isolates have shown to express a capsular polysaccharide known as <i>B. thailandensis</i> capsular variant (BTCV), but human infection has not previously been reported. Although <i>B. thailandenisis</i> has been identified in environmental samples in Laos before, there have not been any human cases reported.</p><p><strong>Case: </strong>A 44-year-old man presented to a district hospital in Laos with a short history of fever and pain in his left foot. Physical examination identified a deep soft-tissue abscess in his left foot and an elevated white blood count. A deep pus sample was taken and melioidosis was suspected from preliminary laboratory tests. The patient was initially started on cloxacillin, ceftriaxone and metronidazole, and was then changed to ceftazidime treatment following local melioidosis treatment guidelines.</p><p><strong>Laboratory methods: </strong>A deep pus sample was sent to Mahosot Hospital microbiology laboratory where a mixed infection was identified including <i>Burkholderia</i> sp. Conventional identification tests and API 20NE were inconclusive, and the <i>B. pseudomallei</i>-specific latex agglutination was positive. The isolate then underwent a <i>Burkholderia</i> species specific PCR which identified the isolate as <i>B. thailandensis.</i> The isolate was sent for sequencing on the Illumina NovaSeq 6000 system and multi-locus sequence typing analysis identified the isolate had the same sequence type (ST696) as <i>B. thailandensis</i> E555, a strain which expresses a <i>B. pseudomallei</i>-like capsular polysaccharide.</p><p><strong>Conclusion: </strong>This is the first report of human infection with <i>B. thailandensis</i> in Laos, and the first report of any human infection with the <i>B. thailandensis</i> capsular variant. Due to the potential for laboratory tests to incorrectly identify this bacteria, staff in endemic areas for <i>B. thailandensis</i> and <i>B. pseudomallei</i> should be aware and ensure that appropriate confirmatory methods are used to differentiate between the species.</p>","PeriodicalId":23677,"journal":{"name":"Wellcome Open Research","volume":"9 ","pages":"421"},"PeriodicalIF":0.0,"publicationDate":"2024-11-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11377925/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142156110","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The genome sequence of the harlequin ladybird, <i>Harmonia axyridis</i> (Pallas, 1773).","authors":"Douglas Boyes, Liam M Crowley","doi":"10.12688/wellcomeopenres.17349.2","DOIUrl":"10.12688/wellcomeopenres.17349.2","url":null,"abstract":"<p><p>We present a genome assembly from an individual female <i>Harmonia axyridis</i> (the harlequin ladybird; Arthropoda; Insecta; Coleoptera; Coccinellidae). The genome sequence is 426 megabases in span. The majority (99.98%) of the assembly is scaffolded into 8 chromosomal pseudomolecules, with the X sex chromosome assembled.</p>","PeriodicalId":23677,"journal":{"name":"Wellcome Open Research","volume":"6 ","pages":"300"},"PeriodicalIF":0.0,"publicationDate":"2024-11-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11589414/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142733093","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"testCompareR: an R package to compare two binary diagnostic tests using paired data.","authors":"Kyle J Wilson, José A Roldán-Nofuentes, Marc Y R Henrion","doi":"10.12688/wellcomeopenres.22411.3","DOIUrl":"10.12688/wellcomeopenres.22411.3","url":null,"abstract":"<p><strong>Background: </strong>Binary diagnostic tests are commonly used in medicine to answer a question about a patient's clinical status, most commonly, do they or do they not have some disease. Recent advances in statistical methodologies for performing inferential statistics to compare commonly used test metrics for two diagnostic tests have not yet been implemented in a statistical package.</p><p><strong>Methods: </strong>Up-to-date statistical methods to compare the test metrics achieved by two binary diagnostic tests are implemented in the new R package testCompareR. The output and efficiency of testCompareR is compared to the only other available package which performs this function, DTComPair, as well as an open-source program, compbdt, using a motivating example.</p><p><strong>Results: </strong>testCompareR achieves similar results to DTComPair using statistical methods with improved coverage and asymptotic performance. Further, testCompareR is faster than the currently available package and requires fewer pre-processing steps in order to produce accurate results.</p><p><strong>Conclusions: </strong>testCompareR provides a new tool to compare the test metrics for two binary diagnostic tests compared with the gold standard. This tool allows flexible inputs, which minimises the need for data pre-processing, and operates in very few steps, so that it is easy to use even for those less experienced with R. testCompareR achieves results comparable to those computed by DTComPair, using optimised statistical methods and with improved computational efficiency.</p>","PeriodicalId":23677,"journal":{"name":"Wellcome Open Research","volume":"9 ","pages":"351"},"PeriodicalIF":0.0,"publicationDate":"2024-11-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11535492/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142581182","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Association between type 2 diabetes and periodontitis: a population-based study in the North Peru.","authors":"Marcela Mayta-Mayorga, Victoria Guerra-Rodríguez, Antonio Bernabe-Ortiz","doi":"10.12688/wellcomeopenres.23036.2","DOIUrl":"10.12688/wellcomeopenres.23036.2","url":null,"abstract":"<p><strong>Background: </strong>Periodontitis, one of the most common forms of periodontal disease, has been linked to several cardiovascular factors including metabolic syndrome and inflammatory processes. This study aimed to determine the association between type 2 diabetes mellitus (T2DM) and periodontitis in a representative sample of individuals in the north of Peru.</p><p><strong>Materials and methods: </strong>Secondary data analysis using information of a population-based survey, enrolling subjects aged 35 to 69 years. The outcome was periodontitis, evaluated using a self-reported and validated 8-item questionnaire (≥5 points compatible with severe periodontitis), whereas the exposure was the presence of T2DM, evaluated using results of oral glucose tolerance test and categorized into two different forms: (a) normoglycemic, prediabetes, and T2DM, and (b) without T2DM, with T2DM and <5 years of diagnosis, and with T2DM and ≥5 years of diagnosis. Poisson regression models were utilized to report prevalence ratios (PR) and 95% confidence intervals (95% CI).</p><p><strong>Results: </strong>Data from 1606 individuals were analyzed, with a mean age of 48.2 (SD: 10.6) years, and 50.3% were women. Of these, 272 (16.9%) had prediabetes and 176 (11.0%) had T2DM (71.6% with <5 years of disease). Overall, 97.0% presented at least one symptom compatible with periodontitis, 882 (55.0%) had mild, 643 (40.0%) had moderate, and 5% had severe periodontitis. In multivariable model, those with T2DM had a higher prevalence of severe periodontitis (PR = 1.99; 95% CI: 1.12 - 3.54). Similarly, those with <5 years of disease had a higher prevalence of severe periodontitis (PR = 2.48; 95% CI: 1.38 - 4.46).</p><p><strong>Conclusions: </strong>Our research confirms the association between T2DM and severe periodontitis, especially among those with recent diagnosis (<5 years). Symptoms of periodontitis are quite common in our study population. Our results suggest a need to periodically assess oral health in patients with T2DM.</p>","PeriodicalId":23677,"journal":{"name":"Wellcome Open Research","volume":"9 ","pages":"562"},"PeriodicalIF":0.0,"publicationDate":"2024-11-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11586918/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142717233","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Human placental cells are resistant to SARS-CoV-2 infection and replication.","authors":"Nagisa Yoshida, Jake R Thomas, Anna Appios, Matthew P Brember, Irving L M H Aye, James R Edgar, Andrew E Firth, Betty Y W Chung, Naomi McGovern, Hazel Stewart","doi":"10.12688/wellcomeopenres.20514.2","DOIUrl":"10.12688/wellcomeopenres.20514.2","url":null,"abstract":"<p><strong>Background: </strong>Infection during pregnancy with SARS-CoV-2 can have a serious impact on both maternal and foetal health. Clinical studies have shown that SARS-CoV-2 transmission from the mother to the foetus typically does not occur. However, there is evidence that SARS-CoV-2 can infect the placenta <i>in utero</i>. Here we sought to quantify the permissiveness of placental cells to SARS-CoV-2 infection and to determine if they support viral release.</p><p><strong>Methods: </strong>By using publicly available single-cell RNA sequencing (scRNAseq) data sets and confocal microscopy we compared ACE2 transcript and protein expression across human first trimester and term placental cells. We also used <i>in vitro</i> infection assays to quantify the infection rates of a range of placenta-derived cells. Finally, we quantified the viral egress from these cells.</p><p><strong>Results: </strong>ACE2 transcripts are found in a range of placental cell types across gestation, including trophoblast. However, ACE2 protein expression does not significantly change across placental cell types from first trimester to term. We find that 0.5±0.15 % of term trophoblast cells can be infected with SARS-CoV-2 while primary placental fibroblasts and macrophages, and JEG-3, JAR and HUVEC cell lines are resistant to infection. Furthermore, primary trophoblast cells poorly support viral release while JEG-3 cells allow relatively high levels of viral release.</p><p><strong>Conclusions: </strong>The low level of viral release by primary placental cells provides insight into how the virus is impaired from crossing the placenta to the foetus.</p>","PeriodicalId":23677,"journal":{"name":"Wellcome Open Research","volume":"9 ","pages":"209"},"PeriodicalIF":0.0,"publicationDate":"2024-11-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11617822/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142787118","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The genome sequence of barren brome, <i>Bromus sterilis</i> L. (Poaceae).","authors":"Maarten J M Christenhusz","doi":"10.12688/wellcomeopenres.22994.1","DOIUrl":"10.12688/wellcomeopenres.22994.1","url":null,"abstract":"<p><p>We present a genome assembly from an individual <i>Bromus sterilis</i> (the barren brome; Streptophyta; Magnoliopsida; Poales; Poaceae). The genome sequence has a total length of 2,677.90 megabases. Most of the assembly is scaffolded into 7 chromosomal pseudomolecules. The mitochondrial and plastid genome assemblies have lengths of 523.28 kilobases and 136.96 kilobases, respectively. Gene annotation of this assembly on Ensembl identified 29,147 protein-coding genes.</p>","PeriodicalId":23677,"journal":{"name":"Wellcome Open Research","volume":"9 ","pages":"534"},"PeriodicalIF":0.0,"publicationDate":"2024-11-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11462126/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142393771","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}