Wellcome Open Research最新文献

{"title":"The genome sequence of the eyed flat-backed millipede, <i>Nanogona polydesmoides</i> (Leach, 1814).","authors":"Christian Owen, Olga Sivell, Duncan Sivell, Richard J Twitchett, Gregory D Edgecombe","doi":"10.12688/wellcomeopenres.24341.2","DOIUrl":"10.12688/wellcomeopenres.24341.2","url":null,"abstract":"<p><p>We present a genome assembly from a specimen of <i>Nanogona polydesmoides</i> (eyed flat-backed millipede; Arthropoda; Diplopoda; Chordeumatida; Craspedosomatidae). The genome sequence has a total length of 406.26 megabases. Most of the assembly (95.49%) is scaffolded into 16 chromosomal pseudomolecules. The mitochondrial genome has also been assembled, with a length of 16.55 kilobases.</p>","PeriodicalId":23677,"journal":{"name":"Wellcome Open Research","volume":"10 ","pages":"332"},"PeriodicalIF":0.0,"publicationDate":"2026-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12334921/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144817638","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Perspectives on public health interventions in the management of the COVID-19 pandemic in Thailand.","authors":"Wirichada Pan-Ngum, Tassawan Poomchaichote, Pimnara Peerawaranun, Natinee Kulpijit, Anne Osterrieder, Naomi Waithira, Mavuto Mukaka, Bhensri Naemiratch, Rita Chanviriyavuth, Supa-At Asarath, Supanat Ruangkajorn, Noppadon Kannika, Phaik Yeong Cheah","doi":"10.12688/wellcomeopenres.16293.4","DOIUrl":"10.12688/wellcomeopenres.16293.4","url":null,"abstract":"<p><strong>Background: </strong>Any government needs to react quickly to a pandemic and make decisions on healthcare interventions locally and internationally with little information regarding the perceptions of people and the reactions they may receive during the implementation of restrictions.</p><p><strong>Methods: </strong>We report an anonymous online survey in Thailand conducted in May 2020 to assess public perceptions of three interventions in the Thai context: isolation, quarantine and social distancing. A total of 1,020 participants, of whom 52% were women, responded to the survey.</p><p><strong>Results: </strong>Loss of income was the main concern among respondents (>80% for all provinces in Thailand). Traditional media and social media were important channels for communication during the pandemic. A total of 92% of respondents reported that they changed their social behaviour even before the implementation of government policy with 94% reporting they performed social distancing, 97% reported using personal protective equipment such as masks and 95% reported using sanitizer products.</p><p><strong>Conclusions: </strong>This study showed a high level of compliance from individuals with government enforced or voluntarily controls such as quarantine, isolation and social distancing in Thailand. The findings from this study can be used to inform future government measures to control the pandemic and to shape communication strategies.</p>","PeriodicalId":23677,"journal":{"name":"Wellcome Open Research","volume":" ","pages":"245"},"PeriodicalIF":0.0,"publicationDate":"2026-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8283548.2/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39285856","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The genome sequence of the Yellow Meadow Ant, <i>Lasius flavus</i> (Fabricius, 1782) (Hymenoptera: Formicidae).","authors":"Gavin R Broad","doi":"10.12688/wellcomeopenres.27332.2","DOIUrl":"10.12688/wellcomeopenres.27332.2","url":null,"abstract":"<p><p>We present a genome assembly from an individual female <i>Lasius flavus</i> (Yellow Meadow Ant; Arthropoda; Insecta; Hymenoptera; Formicidae). The assembly contains two haplotypes with total lengths of 314.61 megabases and 325.88 megabases. Most of haplotype 1 (95.46%) and haplotype 2 (92.16%) are each scaffolded into 15 chromosomal pseudomolecules. The mitochondrial genome has also been assembled, with a length of 21.36 kilobases. This assembly was generated as part of the Darwin Tree of Life project, which produces genomes for eukaryotic species found in Britain and Ireland.</p>","PeriodicalId":23677,"journal":{"name":"Wellcome Open Research","volume":"11 ","pages":"548"},"PeriodicalIF":0.0,"publicationDate":"2026-10-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13658995/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149184734","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The genome sequence of the daisy, <i>Bellis perennis</i> L. (Asterales: Asteraceae).","authors":"Maarten J M Christenhusz, Michael F Fay, Ilia J Leitch","doi":"10.12688/wellcomeopenres.28039.1","DOIUrl":"10.12688/wellcomeopenres.28039.1","url":null,"abstract":"<p><p>We present a genome assembly of <i>Bellis perennis</i> (daisy; Streptophyta; Magnoliopsida; Asterales; Asteraceae). The assembly consists of two haplotypes with total lengths of 1,602.72 megabases and 1,503.20 megabases. Most of the haplotype 1 assembly (93.94%) was scaffolded into nine chromosomal pseudomolecules. Haplotype 2 was assembled to scaffold level. The mitochondrial genome assembly has a length of 262.08 kilobases and the plastid genome assembly has a length of 151.87 kilobases. This assembly was generated as part of the Darwin Tree of Life project, which produces reference genomes for eukaryotic species found in Britain and Ireland.</p>","PeriodicalId":23677,"journal":{"name":"Wellcome Open Research","volume":"11 ","pages":"798"},"PeriodicalIF":0.0,"publicationDate":"2026-10-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13653108/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149167077","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The genome sequence of the Dwarf Willow, <i>Salix herbacea</i> L. (Malpighiales: Salicaceae).","authors":"Markus Ruhsam, Peter M Hollingsworth","doi":"10.12688/wellcomeopenres.28051.1","DOIUrl":"10.12688/wellcomeopenres.28051.1","url":null,"abstract":"<p><p>We present a genome assembly of <i>Salix herbacea</i> (Dwarf Willow; Streptophyta; Magnoliopsida; Malpighiales; Salicaceae). The primary assembly has a total length of 421.43 megabases. Most of the primary assembly (92.79%) is scaffolded into 19 chromosomal pseudomolecules. The mitochondrial genome assembly has a length of 692.62 kilobases and the plastid genome assembly has a length of 155.63 kilobases. This assembly was generated as part of the Darwin Tree of Life project, which produces reference genomes for eukaryotic species found in Britain and Ireland.</p>","PeriodicalId":23677,"journal":{"name":"Wellcome Open Research","volume":"11 ","pages":"808"},"PeriodicalIF":0.0,"publicationDate":"2026-10-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13675631/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149220942","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The genome sequence of the Warted Knot-Horn moth, <i>Acrobasis repandana</i> Fabricius, 1798.","authors":"Douglas Boyes, Inez Januszczak, Ryan Mitchell","doi":"10.12688/wellcomeopenres.23665.2","DOIUrl":"10.12688/wellcomeopenres.23665.2","url":null,"abstract":"<p><p>We present a genome assembly from an individual female specimen of <i>Acrobasis repandana</i> (Warted Knot-Horn moth; Arthropoda; Insecta; Lepidoptera; Pyralidae). The genome sequence has a total length of 620.40 megabases. Most of the assembly (99.78%) is scaffolded into 32 chromosomal pseudomolecules, including the Z and W sex chromosomes. The mitochondrial genome has also been assembled and is 15.21 kilobases in length. Gene annotation of this assembly on Ensembl identified 11,522 protein-coding genes.</p>","PeriodicalId":23677,"journal":{"name":"Wellcome Open Research","volume":"10 ","pages":"50"},"PeriodicalIF":0.0,"publicationDate":"2026-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12004066/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144002104","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Process evaluation protocol of a trial evaluating app-based post knee arthroplasty telerehabilitation strategy in India.","authors":"Siaa Girotra, Purnima Shrivastava, Sandhya Kanaka Yatirajula, Devarsetty Praveen, Ruchika Madan, Seema Grover, Ajit Kumar, Sahil Batra, Bhavuk Garg, Rajesh Malhotra, Ralph Maddison, Niveditha Devasenapathy","doi":"10.12688/wellcomeopenres.26007.3","DOIUrl":"10.12688/wellcomeopenres.26007.3","url":null,"abstract":"<p><strong>Background: </strong>Physical rehabilitation after knee arthroplasty is essential for improving functional outcomes. However, adherence to rehabilitation is poor. We developed a multicomponent mobile application TeleREhabilitation after knee ArThroplasty (TReAT app) comprising education, exercise videos, an e-diary for self-monitoring progress, two-way communication, and remote therapy planning to facilitate home-based rehabilitation. The TReAT trial is a 1:1 randomized controlled superiority trial to evaluate the clinical and cost-effectiveness of the intervention against usual care, with an embedded process evaluation. This paper reports the protocol for process evaluation.</p><p><strong>Methods and analysis: </strong>Consenting individuals undergoing knee arthroplasty at a public and private tertiary care hospital in Delhi, who are able to use a smartphone will be randomized to either app-based or usual home-based rehabilitation and followed up at 3 and 6 months to assess knee-related functional outcomes. A mixed-methods convergent parallel design supported by a logic model will be used for a process evaluation. Data from screening log, backend app analytics, interviews with end users, and researcher notes will contribute to the evaluation based on RE-AIM framework. Researcher notes will be quantified and, consolidated with app analytics to summarise fidelity, and adoption. Thematic analysis of interviews will provide insights into contextual factors influencing intervention adoption during the trial and intention to use the TReAT app beyond the trial period. To explore potential association between use of intervention components and outcomes, correlations between functional outcomes, knowledge score and rehabilitation self-efficacy will be examined.</p><p><strong>Conclusions: </strong>The process evaluation will provide insights into the potential uptake and scalability of TReAT intervention. Comparing findings across public and private healthcare settings will enhance understanding of how the intervention functions in different contexts. The findings will inform implementation strategies to improve rehabilitation care using mHealth interventions in India.</p><p><strong>Trial registration: </strong>The trial was registered prospectively with the Clinical Trial Registry of India (CTRI/2024/06/068838).</p>","PeriodicalId":23677,"journal":{"name":"Wellcome Open Research","volume":"11 ","pages":"207"},"PeriodicalIF":0.0,"publicationDate":"2026-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13560626/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148956615","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The genome sequence of the Marsh Grey, <i>Eudonia pallida</i> (Curtis, 1827) (Lepidoptera: Crambidae).","authors":"Charlotte J Wright, Witold Morek","doi":"10.12688/wellcomeopenres.27972.1","DOIUrl":"10.12688/wellcomeopenres.27972.1","url":null,"abstract":"<p><p>We present a genome assembly from an individual male <i>Eudonia pallida</i> (Marsh Grey; Arthropoda; Insecta; Lepidoptera; Crambidae). The assembly contains two haplotypes with total lengths of 921.55 megabases and 921.62 megabases. The haplotype 1 assembly and the haplotype 2 assembly each contain 18 chromosomal pseudomolecules, including the Z sex chromosome; these account for 99.89% and 99.72% of their respective sequences. The mitochondrial genome has also been assembled, with a length of 15.29 kilobases. Gene annotation of the haplotype 1 assembly by Ensembl identified 12,359 protein-coding genes. This assembly was generated as part of the Darwin Tree of Life project, which produces genomes for eukaryotic species found in Britain and Ireland.</p>","PeriodicalId":23677,"journal":{"name":"Wellcome Open Research","volume":"11 ","pages":"775"},"PeriodicalIF":0.0,"publicationDate":"2026-09-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13662384/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149196793","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The genome sequence of the Garden Ectemnius, <i>Ectemnius cavifrons</i> (Thomson, 1870) (Hymenoptera: Crabronidae).","authors":"Liam M Crowley","doi":"10.12688/wellcomeopenres.27971.1","DOIUrl":"10.12688/wellcomeopenres.27971.1","url":null,"abstract":"<p><p>We present a genome assembly from an individual male <i>Ectemnius cavifrons</i> (Garden Ectemnius; Arthropoda; Insecta; Hymenoptera; Crabronidae). The genome sequence has a total length of 225.31 megabases. Most of the assembly (93.48%) is scaffolded into 14 chromosomal pseudomolecules. The mitochondrial genome has also been assembled, with a length of 21.69 kilobases. Gene annotation of the haploid assembly by Ensembl identified 10,644 protein-coding genes. This assembly was generated as part of the Darwin Tree of Life project, which produces genomes for eukaryotic species found in Britain and Ireland.</p>","PeriodicalId":23677,"journal":{"name":"Wellcome Open Research","volume":"11 ","pages":"774"},"PeriodicalIF":0.0,"publicationDate":"2026-09-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13675622/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149220872","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Strengthening systems to monitor antibiotics. An action oriented approach.","authors":"Olafur Valsson, Elizabeth Tayler, Freddy Eric Kitutu, Jennifer Bonnah, Javiera Cornejo, Alejandro Dorado Garcia, Anahí Dreser, Aitziber Echeverria, Sabiha Y Essack, Stanley Fenwick, Walter Fuller, Nada Hanna, Jennifer Hegewisch-Taylor, Bronwen Holloway, Morgan Jeannin, Jyoti Joshi, Henson Kainga, Amit Khurana, Shaffi Fazaludeen Koya, D G Joakim Larsson, Angkana Lekagul, Jane Lwoyero, Sophie Masika, Ana L P Mateus, Collins Mitambo, Nyambura Moremi, Dishon Muloi, Mwapu Ndahi, Mary Nkansa, Joseph Nkhoma, Sharon Odeo, Pascale Ondoa, Mike Sharland, Renata Tigulini de Souza Bogo, Susanna Sternberg-Lewerin, Marie Verhaegen, David Verner-Jeffreys, J Scott Weese, Kaunda Yamba, S M Sabrina Yesmin, Janet Midega, Clare I R Chandler","doi":"10.12688/wellcomeopenres.24568.2","DOIUrl":"10.12688/wellcomeopenres.24568.2","url":null,"abstract":"<p><strong>Background: </strong>Antibiotics have become lynchpins of our modern systems of healthcare, animal health and agriculture. Monitoring the types and volumes of antibiotics distributed, used and discharged across these systems is critical to provide evidence for action. With limited resources, implementation of multiple tools and recommendations for antibiotic monitoring is challenging. This paper sets out principles and case study illustrations for establishing systems to monitor antibiotic supply and use in resource-constrained settings.</p><p><strong>Methods: </strong>A technical working group and wider reference group drew together expertise and experience from 71 individuals representing different countries, continents and One Health domains, to establish the value of monitoring antibiotics, review tools and guidelines, share experiences and generate principles for prioritising action. This included a literature review, in person workshop, online meetings and collaborative writing between August 2024 and May 2025.</p><p><strong>Results: </strong>The literature search identified two types of outputs: a) antibiotic monitoring and surveillance tools (covering data collection-oriented and residue control tools), and b) guidelines, standards, and mapping/assessment tools for monitoring antibiotics. The group identified multiple purposes for establishing a coordinated system for monitoring antibiotic use across human, animal, plant and environmental domains at country level, including ensuring access, and informing efforts to mitigate antimicrobial resistance (AMR). Tools to monitor use closer to end-user level were less standardised and often more complex. Proposed steps for establishing national antibiotic monitoring systems include starting where the greatest impact can be anticipated in a given context and alignment with other programmes.</p><p><strong>Conclusions: </strong>The agricultural, population, epidemiological, cultural and economic contexts of different countries mean different starting points for establishing antibiotic monitoring. This project characterised an <i>action-oriented prioritisation</i> approach, targeting collection and collation of antibiotic data that have the highest likelihood of affecting change that can achieve impact. Such an approach is risk-based, prioritising monitoring of the antibiotic use that poses greatest risks as locally defined and is feasible to change; sustainable, aligning local expertise, infrastructure and technology with other country priorities; and transparent, ensuring evidence availability within the system alongside reporting 'up and out.' Effective national antibiotic monitoring systems require a collaborative and coordinated strategy focusing on data for action.</p>","PeriodicalId":23677,"journal":{"name":"Wellcome Open Research","volume":"11 ","pages":"55"},"PeriodicalIF":0.0,"publicationDate":"2026-09-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13126008/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147821356","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}