Veterinary Research最新文献

{"title":"Correction: Lumpy skin disease virus suppresses the antiviral response of bovine peripheral blood mononuclear cells that support viral dissemination.","authors":"Manoj Kumar, Ohad Frid, Asaf Sol, Alexander Rouvinski, Sharon Karniely","doi":"10.1186/s13567-025-01544-6","DOIUrl":"10.1186/s13567-025-01544-6","url":null,"abstract":"","PeriodicalId":23658,"journal":{"name":"Veterinary Research","volume":"56 1","pages":"117"},"PeriodicalIF":3.7,"publicationDate":"2025-06-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12164193/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144286623","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Highly suitable LFBK cells for African swine fever virus replication and type I interferon-induced immune studies.","authors":"Eun-Gyeong Lee, Sang-Min Kang, Dongseob Tark","doi":"10.1186/s13567-025-01543-7","DOIUrl":"10.1186/s13567-025-01543-7","url":null,"abstract":"<p><p>African swine fever virus (ASFV), the causative agent of African swine fever, is a fatal haemorrhagic virus affecting domestic pigs and wild boars. The primary target cells for ASFV infection are porcine alveolar macrophages (PAMs); however, PAM isolation and maintenance are technically challenging, and genetic manipulation of these cells is difficult. The lack of suitable cell lines that support ASFV infection and replication has significantly hindered vaccine development. This study aimed to overcome these limitations and advance ASFV research. The results demonstrate that the foetal porcine kidney cells (LFBK) are suitable for ASFV studies. We observed that ASFV replicated significantly more efficiently in LFBK cells than in PAMs. Furthermore, LFBK cells exhibited antiviral immune responses similar to PAMs following ASFV infection or DNA analog. These findings suggest that the LFBK cell line could provide a much-needed platform for studying ASFV replication and pathogenesis while serving as a valuable tool for understanding ASFV-induced immune mechanisms.</p>","PeriodicalId":23658,"journal":{"name":"Veterinary Research","volume":"56 1","pages":"116"},"PeriodicalIF":3.7,"publicationDate":"2025-06-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12153162/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144276039","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development and evaluation of an attenuated Avibacterium paragallinarum strain as a live vaccine candidate for infectious coryza.","authors":"Mengjiao Guo, Haonan Wang, Donghui Liu, Zongyi Bo, Chengcheng Zhang, Yantao Wu, Xiaorong Zhang","doi":"10.1186/s13567-025-01546-4","DOIUrl":"10.1186/s13567-025-01546-4","url":null,"abstract":"<p><p>Avibacterium paragallinarum (Av. paragallinarum), the causative agent of infectious coryza, is a significant pathogen responsible for substantial economic losses in the poultry industry. Current preventive strategies rely primarily on inactivated vaccines, which have limitations such as vaccine failure and limited cross-protection between serotypes. This study aimed to develop an attenuated strain of Av. paragallinarum as a potential live vaccine candidate. Using the Tn5-Kan transposon, we constructed a transposon mutant library and identified a mutant strain, designated 2019/HB64-40, which harbored a disrupted ksgA gene encoding a critical enzyme involved in ribosomal RNA methylation. Compared with the wild-type strain, the 2019/HB64-40 strain presented significantly reduced biofilm formation, lower hemagglutination titres, and impaired growth. Pathogenicity assessments in chickens demonstrated that the mutant strain displayed significantly attenuated virulence, characterized by fewer clinical symptoms and reduced bacterial shedding. Furthermore, following challenge, all unimmunized chickens presented severe clinical signs of infectious coryza at 2 dpi, with symptoms beginning to ameliorate by 5 dpi, culminating in a mean clinical sign score of 2.1. In contrast, only one chicken (1/10) in the immunized group displayed mild facial swelling and nasal discharge, with a mean clinical sign score of 0.1. The immunized group receiving the 2019/HB64-40 strain demonstrated 90% immunoprotection, highlighting the potential of this attenuated strain as a live vaccine candidate. While cross-serotype protection was not evaluated, the results suggest effective homologous protection and colonization capacity, underscoring its promising application in the prevention and treatment of infectious coryza.</p>","PeriodicalId":23658,"journal":{"name":"Veterinary Research","volume":"56 1","pages":"115"},"PeriodicalIF":3.7,"publicationDate":"2025-06-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12150455/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144258978","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"E627V mutation in PB2 protein promotes the mammalian adaptation of novel H10N3 avian influenza virus.","authors":"Meishan Song, Jianyu Liang, Sige Wang, Ruyi Gao, Xiaolong Lu, Wenhao Yang, Yu Chen, Jingxia Ma, Min Gu, Jiao Hu, Xiaowen Liu, Shunlin Hu, Xiaoquan Wang, Kaituo Liu, Xiufan Liu","doi":"10.1186/s13567-025-01534-8","DOIUrl":"10.1186/s13567-025-01534-8","url":null,"abstract":"<p><p>Since 2021, the novel H10N3 has caused four cases of human infection in China, the most recent of which occurred in December 2024, posing a potential threat to public health. Our previous studies indicated that several avian H10N3 strains are highly pathogenic in mice and can be transmitted between mammals via respiratory droplets without prior adaptation. By analyzing the genome sequence, we found that these H10N3 viruses carry the PB2-E627V mutation, which is becoming increasingly common in several subtypes of avian influenza viruses (AIV); however, its mechanism in mammalian adaptation remains unclear. Using a reverse genetics system, we investigated the role of PB2-E627V in the adaptation of H10N3 to mammals and poultry. Our findings demonstrate that the PB2-E627V mutation is critical for the high pathogenicity of novel H10N3 in mice and its ability to be transmitted through the air among mammals. Additionally, we found that the role of PB2-627 V in promoting AIV adaptation to mammals is comparable to that of PB2-627 K. More importantly, PB2-627 V appears to be equally suited to long-term persistence in poultry. Therefore, using PB2-627 V as a novel molecular marker to assess the epidemic potential of AIV is of great significance for preventing possible influenza pandemics in the future.</p>","PeriodicalId":23658,"journal":{"name":"Veterinary Research","volume":"56 1","pages":"111"},"PeriodicalIF":3.7,"publicationDate":"2025-06-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12145617/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144249782","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Swine influenza A virus infection sets the local immunological landscape in subsequent infection with porcine reproductive and respiratory syndrome virus.","authors":"Janaïna Grevelinger, Olivier Bourry, Selma Schmidt, François Meurens, Céline Deblanc, Caroline Hervet, Aline Perrin, Stéphane Gorin, Mireille Le Dimna, Stéphane Quéguiner, Thibaut Larcher, Patricia Renson, Frédéric Paboeuf, Wilhelm Gerner, Nicolas Bertho, Gaëlle Simon","doi":"10.1186/s13567-025-01536-6","DOIUrl":"10.1186/s13567-025-01536-6","url":null,"abstract":"<p><p>Farmed pigs are frequently exposed to respiratory infections, with swine influenza A virus (swIAV) and porcine reproductive and respiratory syndrome virus (PRRSV) being key drivers. Most co-infection studies with these viruses have focused on PRRSV infection followed by swIAV. However, the reverse scenario, where swIAV is given first and then PRRSV, has not been explored. This infection sequence is plausible under natural conditions and warrants further study, especially given that influenza A virus has been shown in mice to impair alveolar macrophages, which are the target cells for PRRSV. This study aimed to evaluate the impact of swIAV infection on the alveolar macrophage population, clinical signs, immune responses, and viral loads during a secondary infection with PRRSV initiated 7 days after the initial swIAV exposure. Results demonstrated that primary swIAV infection did not exacerbate the clinical progression of PRRSV infection, nor did it result in significant differences in PRRSV loads or affect the alveolar macrophage population in the lungs of super-infected pigs as compared to those of pigs infected with PRRSV alone. However, swIAV pre-infection was associated with an increase in the number of conventional dendritic cells type 1 (cDC1), perforin-expressing T cells and NK-related lymphocytes in bronchoalveolar lavage. This coincided with an increase of PRRSV-specific IFN-γ producing CD4 T cells in blood detected 7 days post-PRRSV infection. These findings suggest that a swIAV infection could enhance immune responses during subsequent PRRSV infection by recruiting cDC1 and inducing IL-12, promoting a type-1 immune response, highlighting the complex interplay and often unexpected outcomes of viral co-infections occurring in close temporal proximity.</p>","PeriodicalId":23658,"journal":{"name":"Veterinary Research","volume":"56 1","pages":"114"},"PeriodicalIF":3.7,"publicationDate":"2025-06-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12147356/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144249785","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"PorV factor of the type IX secretion system and PosF porin act as adhesins in Riemerella anatipestifer infection.","authors":"Sen Li, Yanhua Wang, Congran Ning, Rongkun Yang, Yaxin Wu, Xu Cheng, Keke Ren, Minghua Huang, Xiong Liu, Naiji Zhou, Wanpo Zhang, Sishun Hu, Yuncai Xiao, Zili Li, Hongbo Zhou, Zhengfei Liu, Zutao Zhou","doi":"10.1186/s13567-025-01550-8","DOIUrl":"10.1186/s13567-025-01550-8","url":null,"abstract":"<p><p>Riemerella anatipestifer infection is a critical disease that is a major threat to the poultry industry worldwide. The adhesion and invasion of host cells are key steps in the primary stages of bacterial infection. However, the outer membrane proteins that mediate these events in R. anatipestifer are poorly characterized. In this study, the PorV and PosF proteins, as well as the previously described OMP71 protein, were identified as important mediators of the adhesion and invasion of duck embryo fibroblast (DEF) cells by R. anatipestifer. Affinity chromatography-based surface proteomics was used to screen for adhesion proteins. The surface proteins on DEF cells were labelled with biotin-avidin to enrich for outer membrane proteins of R. anatipestifer, which generated 11 candidate proteins that were tested further. Protein adhesion and blocking assays and polyclonal antiserum inhibition analysis revealed that the PorV, PosF, and OMP71 proteins are adhesion factors. Knockout of porV or posF reduced the adhesion and invasion of R. anatipestifer in DEF cells. Moreover, the pathogenicity of the mutant strains was significantly attenuated, which supports the hypothesis that PorV and PosF are important virulence factors required for the pathogenicity of R. anatipestifer. The PorV protein is a key component of the type IX secretory system and is responsible for transporting effector substrates to the extracellular environment, whereas PosF belongs to the porin superfamily of barrel-shaped transmembrane proteins. This is the first description that PorV is an adhesin involved in host‒microbial interactions, which represents a breakthrough in pathogenicity studies of R. anatipestifer and other members of Flavobacteriaceae.</p>","PeriodicalId":23658,"journal":{"name":"Veterinary Research","volume":"56 1","pages":"112"},"PeriodicalIF":3.5,"publicationDate":"2025-06-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12147357/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144249784","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cytotoxicity induced by Aeromonas schubertii is orchestrated by a unique set of type III secretion system effectors.","authors":"Hana Michova, Jan Pliva, Anezka Jirsova, David Jurnecka, Jana Kamanova","doi":"10.1186/s13567-025-01548-2","DOIUrl":"10.1186/s13567-025-01548-2","url":null,"abstract":"<p><p>The type III secretion system (T3SS) is an important virulence factor of Gram-negative bacteria, including the genus Aeromonas, which represents a diverse group of aquatic bacteria. One member of the genus, Aeromonas schubertii, is an emerging pathogen in aquaculture, causing high mortality in snakehead fish. Infections are associated with the formation of white nodules in the internal organs, likely resulting from A. schubertii-induced apoptosis and/or necrosis. The present study investigates the type strain A. schubertii ATCC 43700, which encodes two distinct T3SSs located within Aeromonas pathogenicity islands 1 and 2, referred here to as API1 and API2. We analyzed their role in A. schubertii-induced cytotoxicity and identified novel T3SS effector proteins. Infections of HeLa cells revealed that API1, but not API2, mediates cytotoxicity and induces both apoptotic and necrotic cell death. Moreover, proteomic analysis identified seven candidate effectors secreted by the API1 injectisome. These included two previously described effectors, AopH and AopO from A. salmonicida, as well as five novel effectors named AopI, AopJ, AopL, AopT, and AopU, whose injection into host cells was validated using a split luciferase reporter system. Functional characterization showed that AopL, a homolog of Vibrio parahaemolyticus VopQ, induces caspase-3/-7-independent necrosis, while AopI, a homolog of ExoY from Pseudomonas aeruginosa, suppresses caspase-3/-7 activation and necrosis, revealing a pro-survival function. These results demonstrate the critical role of the API1 injectisome in A. schubertii-induced cytotoxicity and provide experimental identification of novel Aeromonas effectors that cooperate to fine-tune host cell cytotoxicity.</p>","PeriodicalId":23658,"journal":{"name":"Veterinary Research","volume":"56 1","pages":"113"},"PeriodicalIF":3.7,"publicationDate":"2025-06-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12147276/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144249781","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Getah virus nonstructural protein 2 suppresses interferon-beta production by interrupting interferon regulatory factor 3 activation.","authors":"Hua Liu, Zhao Qi, Lan Tian, Zhe Chen, Haonan Li, Le Liu, Sicong Liu, Shuai Li, Jiumeng Sun, Ying Shao, Xiangjun Song, Jian Tu, Liangqiang Zhu, Kezong Qi, Zhenyu Wang","doi":"10.1186/s13567-025-01547-3","DOIUrl":"10.1186/s13567-025-01547-3","url":null,"abstract":"<p><p>Getah virus (GETV), a neglected and re-emerging mosquito-borne alphavirus, has become more serious and poses a potential threat to animal safety and public health. The innate immune response is critical for host defence against viral infection, and the dysregulation of host innate immune responses likely aggravates GETV infection. In this study, we use unbiased screening to identify GETV proteins that antagonise type I interferon (IFN-I) response. We found that GETV Nsp2 could inhibit Sendai virus or poly(I:C)-induced IFN-β promoter activation, potently suppressing primary interferon production- a key component of the host's innate immunity antiviral response. Remarkably, Nsp2 showed efficient inhibition of the IRF3-responsive promoter, but not AP-1 or NF-κB. Further examination revealed that Nsp2 significantly suppressed luciferase activity when RIG-I-CARD, MDA5, MAVS, or IRF3 activated the IFN-β promoter. By contrast, IRF3/5D led to less suppression of luciferase expression, partially restoring luciferase activity, suggesting that Nsp2 interferes with the biological function of IRF3 as a crucial strategy in its antagonism of IFN-β production. Mechanistically, Nsp2 binds TBK1 to suppress IRF3 phosphorylation. Meanwhile, Nsp2 competitively inhibited the interaction of pIRF3 with KPNA3 and KPNA4, to inhibit IRF3 nuclear translocation. Overall, we demonstrated that GETV suppresses antiviral innate immunity by inhibiting the activation of IRF3, and Nsp2 plays a crucial role in this process. These findings reveal a novel strategy by which GETV evades the host innate immune response, providing new insights into the pathogenesis of GETV.</p>","PeriodicalId":23658,"journal":{"name":"Veterinary Research","volume":"56 1","pages":"110"},"PeriodicalIF":3.7,"publicationDate":"2025-06-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12145652/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144249783","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Host-similar fragments in the African swine fever virus genome: distribution, functions, and evolution.","authors":"Zhaozhong Zhu, Na Li, Qin Sun, Xizi Long, Tao Wang, Hua-Ji Qiu","doi":"10.1186/s13567-025-01539-3","DOIUrl":"10.1186/s13567-025-01539-3","url":null,"abstract":"<p><p>African swine fever virus (ASFV) predominantly infects Argasidae and suids, resulting in high morbidity and mortality in pigs. Despite the crucial role that viral sequences resembling those of the host play in the virus's survival, there are limited comprehensive studies on the genomic similarities between ASFV and its hosts. Consequently, this study employs homology analysis to construct a similarity network between ASFV and its hosts (Argasidae and suids), investigating the distribution, function, evolution, and origins of these similar sequences in ASFV. Our findings indicate that the host-similar fragments are mainly distributed between positions 70000 and 180000 of the ASFV genome, primarily within non-coding regions. Notably, these non-coding fragments are often associated with promoter functions. Furthermore, the analysis of suid proteins that share similarities with ASFV proteins reveals that they predominantly exhibit RNA polymerase activity and are involved in metabolic processes. Evolutionary analysis indicates that pan-similar sequences of ASFV exist in an open state, highlighting the diversity of these analogous sequences. Additionally, a positive correlation was identified between the occurrence of recombination breakpoints and similar sequences, indicating that homologous recombination may serve as a crucial mechanism driving the formation of these analogous sequences.</p>","PeriodicalId":23658,"journal":{"name":"Veterinary Research","volume":"56 1","pages":"108"},"PeriodicalIF":3.7,"publicationDate":"2025-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12107907/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144162439","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Post-outbreak dynamics and persistence of Actinobacillus pleuropneumoniae serotype 15 in finisher pigs in Iowa.","authors":"Marcelo Nunes de Almeida, Pablo P Pineyro, Derald Holtkamp, Isadora Machado, Ana P S Silva, Guilherme Cezar, Peter Thomas, Marcelo Gottschalk, Alyona A Michael","doi":"10.1186/s13567-025-01538-4","DOIUrl":"10.1186/s13567-025-01538-4","url":null,"abstract":"<p><p>In 2021, Actinobacillus pleuropneumoniae serotype 15 (App15) mediated a high mortality respiratory outbreak in finisher hogs, affecting multiple companies within a 30-km radius of Iowa, USA. The atypical regional spread raised concerns for the strain's unusual environmental persistence and survivability. This prospective longitudinal study aimed to determine the duration of App15 persistence in convalescent pigs at a naturally infected 1200 head site. Sixty-seven pigs were sampled individually for 6 weeks using nasal swabs (NS), tonsil scrapings (TS), and serum samples (SS); pen-based oral fluids (OF) were also collected. NS, TS, and OF were tested for App by rtPCR, while serum was screened with the Swinecheck mix-App ELISA. All 67 pigs tested App PCR positive in TS ≥ 1 during the sampling period, with progressive increases in detection from 53.3% to 95.8% between the first and last sampling week, respectively. Only 23 pigs (34.3%) were PCR positive by NS ≥ 1 during the sampling period, with decaying detection rates from a peak of 51.2% positivity in the first sampling week. Fifty-three of 73 pens (72.6%) tested App PCR positive in OF ≥ 1 during the sampling period. Seropositivity decreased from 93% on week 4- to 33% on week 8. TS had the highest PCR detection rate at all time points evaluated, representing the best antemortem sample type for App PCR detection in this study. The results reported here generated new important knowledge related to App15 ecology and epidemiology, prospectively informing disease diagnosis, surveillance, and biosecurity practices.</p>","PeriodicalId":23658,"journal":{"name":"Veterinary Research","volume":"56 1","pages":"107"},"PeriodicalIF":3.7,"publicationDate":"2025-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12107770/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144162441","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}