Veterinary Research最新文献_第8页

Glutathione reductase modulates endogenous oxidative stress and affects growth and virulence in Avibacterium paragallinarum. 谷胱甘肽还原酶调节内源性氧化应激并影响副鸡芽鸟杆菌的生长和毒力。

IF 3.7 1区农林科学

Veterinary Research Pub Date : 2025-01-02 DOI: 10.1186/s13567-024-01388-6

Yan Zhi, Chen Mei, Zhenyi Liu, Ying Liu, Hongjun Wang

{"title":"Glutathione reductase modulates endogenous oxidative stress and affects growth and virulence in Avibacterium paragallinarum.","authors":"Yan Zhi, Chen Mei, Zhenyi Liu, Ying Liu, Hongjun Wang","doi":"10.1186/s13567-024-01388-6","DOIUrl":"10.1186/s13567-024-01388-6","url":null,"abstract":"Glutathione reductase (GR) plays a pivotal role in managing oxidative stress, a process crucial for microbial virulence and adaptation, yet it has not been extensively explored in bacteria such as Avibacterium paragallinarum (Av. paragallinarum). This study examined the specific roles of GR in Av. paragallinarum, focusing on how GR modulates the bacterium's response to oxidative stress and impacts its pathogenic behavior. Using gene knockouts together with transcriptomic and metabolomic profiling, we identified an important shift in redox balance due to GR deficiency, which disrupted energy metabolism and weakened the oxidative stress defense, culminating in a notable decline in virulence. In addition, decreased growth rates, reduced biofilm production, and weakened macrophage interactions were observed in GR-deficient strains. Notably, our findings reveal a sophisticated adaptation mechanism wherein the bacterium recalibrated its metabolic pathways in response to GR deficiency without fully restoring virulence. Our in vivo studies further highlight the pivotal role of GR in pathogen fitness. Together, our findings connect GR-mediated redox control to bacterial virulence, thereby furthering the understanding of microbial adaptation and positioning GR as a potential antimicrobial target. Our insights into the GR-centric regulatory network pave the way for leveraging bacterial redox mechanisms in the development of novel antimicrobial therapies, highlighting the importance of oxidative stress management in bacterial pathogenicity.","PeriodicalId":23658,"journal":{"name":"Veterinary Research","volume":"56 1","pages":"1"},"PeriodicalIF":3.7,"publicationDate":"2025-01-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11697956/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142923629","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Correction: Vidofludimus inhibits porcine reproductive and respiratory syndrome virus infection by targeting dihydroorotate dehydrogenase. 更正：Vidofludimus通过靶向二氢根酸脱氢酶抑制猪繁殖与呼吸综合征病毒感染。

IF 3.7 1区农林科学

Veterinary Research Pub Date : 2024-12-27 DOI: 10.1186/s13567-024-01447-y

Yuanqi Yang, Yanni Gao, Lujie Zhang, Xing Liu, Yangyang Sun, Juan Bai, Ping Jiang

引用次数: 0

African swine fever virus enhances viral replication by increasing intracellular reduced glutathione levels, which suppresses stress granule formation. 非洲猪瘟病毒通过增加细胞内还原型谷胱甘肽水平来增强病毒复制，从而抑制应激颗粒的形成。

IF 3.7 1区农林科学

Veterinary Research Pub Date : 2024-12-20 DOI: 10.1186/s13567-024-01433-4

Han Gao, Taoming Gu, Xiaopeng Gao, Zebu Song, Jing Liu, Yi Song, Guihong Zhang, Yankuo Sun

{"title":"African swine fever virus enhances viral replication by increasing intracellular reduced glutathione levels, which suppresses stress granule formation.","authors":"Han Gao, Taoming Gu, Xiaopeng Gao, Zebu Song, Jing Liu, Yi Song, Guihong Zhang, Yankuo Sun","doi":"10.1186/s13567-024-01433-4","DOIUrl":"10.1186/s13567-024-01433-4","url":null,"abstract":"African swine fever virus (ASFV) is a DNA virus that has significantly impacted the global swine industry. Currently, there are no effective therapies or vaccines against ASFV. Stress granules (SGs), known for their antiviral properties, are not induced during ASFV infection, even though reactive oxygen species (ROS) are generated. The mechanism by which ASFV regulates SGs formation remains unclear. This study demonstrates that ASFV antagonises SGs formation and increases intracellular levels of reduced glutathione (GSH) levels. The use of the GSH inhibitor BSO and the activator NAC confirmed that the ASFV-induced increase in GSH helps to suppress SGs formation and influences viral replication. Additionally, this study revealed that ASFV enhances GSH by upregulating the antioxidant transcription factor NRF2, as well as factors involved in GSH synthesis and regeneration, such as GCLC, and those related to the ferroptosis pathway, such as SLC7A11. Furthermore, the study uncovered that ASFV manipulates intracellular GSH levels by activating the mitochondrial protein AIFM1. This regulatory mechanism helps the virus inhibit the formation of intracellular SGs, thereby creating an optimal environment for viral replication. These findings provide new insights into the molecular strategies employed by ASFV.","PeriodicalId":23658,"journal":{"name":"Veterinary Research","volume":"55 1","pages":"172"},"PeriodicalIF":3.7,"publicationDate":"2024-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11662820/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142872851","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A spatially-heterogeneous impact of fencing on the African swine fever wavefront in the Korean wild boar population. 围栏对韩国野猪种群中非洲猪瘟波前的空间异质性影响。

IF 3.7 1区农林科学

Veterinary Research Pub Date : 2024-12-18 DOI: 10.1186/s13567-024-01422-7

Jun-Sik Lim, Timothée Vergne, Eutteum Kim, Claire Guinat, Simon Dellicour, Mathieu Andraud

{"title":"A spatially-heterogeneous impact of fencing on the African swine fever wavefront in the Korean wild boar population.","authors":"Jun-Sik Lim, Timothée Vergne, Eutteum Kim, Claire Guinat, Simon Dellicour, Mathieu Andraud","doi":"10.1186/s13567-024-01422-7","DOIUrl":"10.1186/s13567-024-01422-7","url":null,"abstract":"In October 2019, South Korea's first case of African swine fever (ASF) was reported in wild boar in the north of the country. Despite the implementation of a 2300 km-long fencing strategy, the ASF wavefront continued to invade southward. Our study aimed to investigate the ASF wavefront dynamics in different regions of South Korea, as well as to assess the effectiveness of the fencing measures on ASF dispersal and wavefront velocity. From the nationwide wild boar surveillance system, we extracted 2661 cases, starting from 2 October 2019 (first detection) to 15 September 2022. The cases were categorised into four main spatiotemporal clusters. The average wavefront velocity over the four clusters was estimated at 0.52 km/week, with the cluster in the eastern part of the Korean peninsula exhibiting the fastest velocity (0.99 km/week) compared to the other clusters (0.44, 0.31, and 0.15 km/week). We hypothesise that these differences are related to different wild boar densities due to heterogeneous habitat suitability. We also found that fencing significantly impacted ASF dispersal in only two of the four main clusters, with no evidence that fencing slowed down the spread of the wavefront in any of the clusters. We argue that this heterogeneity might result from fencing locations being misaligned with the true (and unobserved) wavefront.","PeriodicalId":23658,"journal":{"name":"Veterinary Research","volume":"55 1","pages":"163"},"PeriodicalIF":3.7,"publicationDate":"2024-12-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11654197/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142855586","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Developmental patterns of intestinal group 3 innate lymphoid cells in piglets and their response to enterotoxigenic Escherichia coli infection. 仔猪肠道第3组先天淋巴样细胞的发育模式及其对产肠毒素大肠杆菌感染的反应

IF 3.7 1区农林科学

Veterinary Research Pub Date : 2024-12-18 DOI: 10.1186/s13567-024-01418-3

Ningning Huang, Ling Ye, Hao Li, Jian Peng, Hongkui Wei

{"title":"Developmental patterns of intestinal group 3 innate lymphoid cells in piglets and their response to enterotoxigenic Escherichia coli infection.","authors":"Ningning Huang, Ling Ye, Hao Li, Jian Peng, Hongkui Wei","doi":"10.1186/s13567-024-01418-3","DOIUrl":"10.1186/s13567-024-01418-3","url":null,"abstract":"Diarrhoea and preweaning mortality in piglets are crucial factors impacting the economic sustainability of the swine industry. Pathogenic infections are among the main causes of diarrhea and mortality. Group 3 innate lymphoid cells (ILC3s) are crucial for safeguarding against pathogenic infections. However, knowledge regarding the development and function of ILC3s in suckling piglets is currently limited. Our findings demonstrate that the development of ILC3s in suckling piglets gradually progresses from day 1 to day 21, with a notable increase observed on day 28. Additionally, the development of NKp46+ILC3s and the production of interleukin (IL)-17A by ILC3s displayed consistent patterns with the changes observed in ILC3s. Notably, interferon (IFN)-γ levels significantly increased on day 14. Moreover, the production of IFN-γ by NKp46+ILC3s was greater than that by NKp46-ILC3s. Importantly, when piglets were subjected to a 4-h challenge with enterotoxigenic Escherichia coli, both the percentages of ILC3s significantly increased, accompanied by increased IL-22 production, highlighting their importance in maintaining intestinal health. The outcomes of this study provide valuable insights for future related research.","PeriodicalId":23658,"journal":{"name":"Veterinary Research","volume":"55 1","pages":"159"},"PeriodicalIF":3.7,"publicationDate":"2024-12-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11654254/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142855591","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Revealing novel and conservative CD8⁺T-cell epitopes with MHC B2 restriction on ALV-J. 揭示新的和保守的CD8+ t细胞表位与MHC B2限制ALV-J。

IF 3.7 1区农林科学

Veterinary Research Pub Date : 2024-12-18 DOI: 10.1186/s13567-024-01426-3

Xueqing Li, Ziwei Li, Mulin Ma, Na Yang, Shanyao Du, Ming Liao, Manman Dai

{"title":"Revealing novel and conservative CD8+T-cell epitopes with MHC B2 restriction on ALV-J.","authors":"Xueqing Li, Ziwei Li, Mulin Ma, Na Yang, Shanyao Du, Ming Liao, Manman Dai","doi":"10.1186/s13567-024-01426-3","DOIUrl":"10.1186/s13567-024-01426-3","url":null,"abstract":"MHC B2 haplotype chickens have been reported to induce strong immune response against various avian pathogens. However, little is known about the CD8+T-cell epitope with MHC B2-restricted on subgroup J avian leukosis virus (ALV-J). In this study, we explored the ALV-J-induced cellular immune response in B2 haplotype chickens in vivo. We found that ALV-J infection significantly increased the proportion of CD8+T cells in chickens and up-regulated the expression of cytotoxic genes like Granzyme A and antiviral genes like IFIT5 at 14 days post-infection (dpi). We selected 32 candidate peptides based on the peptide-binding motif and further identified three MHC B2-restricted CD8+T epitopes on ALV-J, including Pol652-660, Gag374-382, and Gag403-411 which induced significant levels of chicken IFN-γ production in splenocytes from ALV-J infected chickens using the ELISpot assay. In addition, we also verified that the three identified epitopes stimulated memory splenocytes elevating TNF-α and IL-2 protein expression. Importantly, we found that the three positive peptides were highly conserved among ALV-A, ALV-B, ALV-E, ALV-J, and ALV-K. Taken together, we identified three MHC B2-restricted CD8+T cell epitopes on ALV-J, providing a foundation for developing effective T cell epitope vaccines targeting conserved internal viral proteins.","PeriodicalId":23658,"journal":{"name":"Veterinary Research","volume":"55 1","pages":"164"},"PeriodicalIF":3.7,"publicationDate":"2024-12-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11654158/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142855634","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Insights into genetic determinants of piglet survival during a PRRSV outbreak. PRRSV爆发期间仔猪存活的遗传决定因素的见解。

IF 3.7 1区农林科学

Veterinary Research Pub Date : 2024-12-18 DOI: 10.1186/s13567-024-01421-8

Joaquim Tarrés, Teodor Jové-Juncà, Carles Hernández-Banqué, Olga González-Rodríguez, Llilianne Ganges, Sofia Gol, Marta Díaz, Josep Reixach, Ramona N Pena, Raquel Quintanilla, Maria Ballester

{"title":"Insights into genetic determinants of piglet survival during a PRRSV outbreak.","authors":"Joaquim Tarrés, Teodor Jové-Juncà, Carles Hernández-Banqué, Olga González-Rodríguez, Llilianne Ganges, Sofia Gol, Marta Díaz, Josep Reixach, Ramona N Pena, Raquel Quintanilla, Maria Ballester","doi":"10.1186/s13567-024-01421-8","DOIUrl":"10.1186/s13567-024-01421-8","url":null,"abstract":"Breeding animals to produce more robust and disease-resistant pig populations becomes a complementary strategy to the more conventional methods of biosecurity and vaccination. The objective of this study was to explore the ability of a panel of genetic markers and immunity parameters to predict the survival rates during a natural PRRSV outbreak. Ten-week-old female Duroc pigs (n = 129), obtained from 61 sows and 20 boars, were naturally infected with a highly pathogenic PRRSV genotype 1 strain. Prior to infection, piglets were screened for immunity parameters (IgG levels in plasma and SOX13 mRNA expression in blood) and genetic markers previously associated to PRRSV immune response and immunity traits. Additionally, the 20 boars were genotyped with a panel of 132 single nucleotide polymorphisms (SNPs). Survival analysis showed that mortality was significantly higher for animals with low basal IgG levels in plasma and/or high SOX13 mRNA expression in blood. The genotypes of sires for SNPs associated with IgG plasma levels, CRP in serum, percentage of γδ T cells, lymphocyte phagocytic capacity, total number of lymphocytes and leukocytes, and MCV and MCH were significantly associated with the number of surviving offspring. Furthermore, CD163 and GBP5 markers were also associated to piglet survival. The effects of these SNPs were polygenic and cumulative, survival decreased from 94 to 21% as more susceptible alleles were accumulated for the different markers. Our results confirmed the existence of genetic variability in survival after PRRSV infection and provided a set of genetic markers and immunity traits associated with PRRS resistance.","PeriodicalId":23658,"journal":{"name":"Veterinary Research","volume":"55 1","pages":"160"},"PeriodicalIF":3.7,"publicationDate":"2024-12-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11654192/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142855631","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The F204S mutation in adrenodoxin oxidoreductase drives salinomycin resistance in Eimeria tenella. 肾上腺素还氧素氧化还原酶F204S突变驱动柔嫩艾美球虫耐盐霉素。

IF 3.7 1区农林科学

Veterinary Research Pub Date : 2024-12-18 DOI: 10.1186/s13567-024-01431-6

Pei Sun, Chaoyue Wang, Fujie Xie, Linlin Chen, Yuanyuan Zhang, Xinming Tang, Dandan Hu, Yang Gao, Ning Zhang, Zhenkai Hao, Yonglan Yu, Jingxia Suo, Xun Suo, Xianyong Liu

{"title":"The F204S mutation in adrenodoxin oxidoreductase drives salinomycin resistance in Eimeria tenella.","authors":"Pei Sun, Chaoyue Wang, Fujie Xie, Linlin Chen, Yuanyuan Zhang, Xinming Tang, Dandan Hu, Yang Gao, Ning Zhang, Zhenkai Hao, Yonglan Yu, Jingxia Suo, Xun Suo, Xianyong Liu","doi":"10.1186/s13567-024-01431-6","DOIUrl":"10.1186/s13567-024-01431-6","url":null,"abstract":"Salinomycin is a polyether ionophore widely used for the treatment of coccidiosis in poultry. However, the emergence of coccidia strains resistant to salinomycin presents challenges for control efforts, and the mechanisms underlying this resistance in Eimeria remain inadequately understood. In this study, 78 stable salinomycin-resistant strains were generated through experimental evolution approaches. Whole-genome sequencing of salinomycin-resistant Eimeria tenella isolates revealed single nucleotide polymorphisms (SNPs), with 12 candidate genes harboring nonsynonymous mutations identified. To confirm the candidate gene responsible for conferring salinomycin resistance, we leveraged reverse genetic strategies and identified a key amino acid substitution (F204S) in adrenodoxin oxidoreductase (EtADR), which markedly reduced susceptibility to salinomycin. Our results elucidate the complex interactions among salinomycin resistance, parasite fitness, point mutations, and the structure of EtADR, laying the foundation for future studies on drug resistance in Eimeria and contributing to the development of targeted control strategies.","PeriodicalId":23658,"journal":{"name":"Veterinary Research","volume":"55 1","pages":"170"},"PeriodicalIF":3.7,"publicationDate":"2024-12-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11654014/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142855648","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Revealing novel CD8⁺ T-cell epitopes from the H5N1 avian influenza virus in HBW/B1 haplotype ducks. 在HBW/B1单倍型鸭中发现H5N1禽流感病毒新的CD8+ t细胞表位

IF 3.7 1区农林科学

Veterinary Research Pub Date : 2024-12-18 DOI: 10.1186/s13567-024-01415-6

Wanlin Jiao, Yingyi Chen, Zimin Xie, Li Zhao, Shanyao Du, Mulin Ma, Ming Liao, Manman Dai

{"title":"Revealing novel CD8+ T-cell epitopes from the H5N1 avian influenza virus in HBW/B1 haplotype ducks.","authors":"Wanlin Jiao, Yingyi Chen, Zimin Xie, Li Zhao, Shanyao Du, Mulin Ma, Ming Liao, Manman Dai","doi":"10.1186/s13567-024-01415-6","DOIUrl":"10.1186/s13567-024-01415-6","url":null,"abstract":"The duck CD8+ T-cell response effectively defends against H5N1 highly pathogenic avian influenza virus (HPAIV) infection, but the recognized peptide is rarely identified. Here, we found that the ratio of CD8+ T cells and the expression of IFN-γ and cytotoxicity-associated genes, including granzyme A/K, perforin and IL2, at 7 days post-infection in peripheral blood mononuclear cells (PBMCs) from B1 haplotype ducks significantly increased in the context of defending against H5N1 AIV infection in vivo. Moreover, similar results were observed in cultured and sorted H5N1 AIV-stimulated duck CD8+ T cells in vitro. Next, we selected 109 epitopes as candidate epitopes on the basis of the MHC-I restriction binding peptide prediction website database and further identified twelve CD8+ T-cell epitopes that significantly increased IFN-γ gene expression after stimulating B1 haplotype duck memory PBMCs. In particular, NP338-346, NP473-481, M2-10, PB1540-548 and PA80-88 were highly conserved in H5N1, H5N6, H5N8, H7N9, and H9N2 AIVs. These findings provide directions for the development of universal T-cell epitope vaccines for AIV in ducks.","PeriodicalId":23658,"journal":{"name":"Veterinary Research","volume":"55 1","pages":"169"},"PeriodicalIF":3.7,"publicationDate":"2024-12-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11653964/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142855644","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Heat shock protein 70 enhances viral replication by stabilizing Senecavirus A nonstructural proteins L and 3D. 热休克蛋白70通过稳定赛尼卡病毒A非结构蛋白L和3D增强病毒复制。

IF 3.7 1区农林科学

Veterinary Research Pub Date : 2024-12-18 DOI: 10.1186/s13567-024-01414-7

Lei Hou, Penghui Zeng, Zhi Wu, Xiaoyu Yang, Jinshuo Guo, Yongyan Shi, Jiangwei Song, Jianwei Zhou, Jue Liu

{"title":"Heat shock protein 70 enhances viral replication by stabilizing Senecavirus A nonstructural proteins L and 3D.","authors":"Lei Hou, Penghui Zeng, Zhi Wu, Xiaoyu Yang, Jinshuo Guo, Yongyan Shi, Jiangwei Song, Jianwei Zhou, Jue Liu","doi":"10.1186/s13567-024-01414-7","DOIUrl":"10.1186/s13567-024-01414-7","url":null,"abstract":"Senecavirus A (SVA) is an emerging pathogen that causes idiopathic vesicular infections in pig herds, posing a potential threat to their production performance. Heat shock protein 70 (Hsp70) is a molecular chaperone that plays an important role in host homeostasis under both physiological and stress conditions. However, the effects of Hsp70 on SVA infection and its underlying regulatory mechanisms remain unclear. Here, we confirmed that Hsp70 expression promotes SVA infection, as evidenced by the expression of viral proteins, viral titers, and the number of rSVA-eGFP-infected cells. This positive regulatory role of Hsp70 is mainly involved in post-entry stages of SVA. Viral proteins that interacted with Hsp70 were screened, and co-immunoprecipitation (co-IP) shows an interaction between Hsp70 and SVA L and 3D proteins. Subsequently, we determined that the expression of Hsp70 is beneficial for the stability of the SVA L and 3D proteins. Additionally, the substrate-binding domain (SBD) of Hsp70 plays an important role in the interaction between Hsp70 and SVA L or 3D proteins; and the deletion of this domain results in the loss of the stabilizing effect of Hsp70 on SVA L and 3D proteins and the positive regulatory effect of Hsp70 on SVA replication. These results reveal that Hsp70 promotes SVA infection by stabilizing viral L and 3D proteins and provides a strategy for preventing and controlling SVA infection.","PeriodicalId":23658,"journal":{"name":"Veterinary Research","volume":"55 1","pages":"158"},"PeriodicalIF":3.7,"publicationDate":"2024-12-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11654094/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142855624","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0