Veterinary Research最新文献

A spatially-heterogeneous impact of fencing on the African swine fever wavefront in the Korean wild boar population.

IF 3.7 1区农林科学

Veterinary Research Pub Date : 2024-12-18 DOI: 10.1186/s13567-024-01422-7

Jun-Sik Lim, Timothée Vergne, Eutteum Kim, Claire Guinat, Simon Dellicour, Mathieu Andraud

{"title":"A spatially-heterogeneous impact of fencing on the African swine fever wavefront in the Korean wild boar population.","authors":"Jun-Sik Lim, Timothée Vergne, Eutteum Kim, Claire Guinat, Simon Dellicour, Mathieu Andraud","doi":"10.1186/s13567-024-01422-7","DOIUrl":"https://doi.org/10.1186/s13567-024-01422-7","url":null,"abstract":"In October 2019, South Korea's first case of African swine fever (ASF) was reported in wild boar in the north of the country. Despite the implementation of a 2300 km-long fencing strategy, the ASF wavefront continued to invade southward. Our study aimed to investigate the ASF wavefront dynamics in different regions of South Korea, as well as to assess the effectiveness of the fencing measures on ASF dispersal and wavefront velocity. From the nationwide wild boar surveillance system, we extracted 2661 cases, starting from 2 October 2019 (first detection) to 15 September 2022. The cases were categorised into four main spatiotemporal clusters. The average wavefront velocity over the four clusters was estimated at 0.52 km/week, with the cluster in the eastern part of the Korean peninsula exhibiting the fastest velocity (0.99 km/week) compared to the other clusters (0.44, 0.31, and 0.15 km/week). We hypothesise that these differences are related to different wild boar densities due to heterogeneous habitat suitability. We also found that fencing significantly impacted ASF dispersal in only two of the four main clusters, with no evidence that fencing slowed down the spread of the wavefront in any of the clusters. We argue that this heterogeneity might result from fencing locations being misaligned with the true (and unobserved) wavefront.","PeriodicalId":23658,"journal":{"name":"Veterinary Research","volume":"55 1","pages":"163"},"PeriodicalIF":3.7,"publicationDate":"2024-12-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142855586","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Developmental patterns of intestinal group 3 innate lymphoid cells in piglets and their response to enterotoxigenic Escherichia coli infection.

IF 3.7 1区农林科学

Veterinary Research Pub Date : 2024-12-18 DOI: 10.1186/s13567-024-01418-3

Ningning Huang, Ling Ye, Hao Li, Jian Peng, Hongkui Wei

{"title":"Developmental patterns of intestinal group 3 innate lymphoid cells in piglets and their response to enterotoxigenic Escherichia coli infection.","authors":"Ningning Huang, Ling Ye, Hao Li, Jian Peng, Hongkui Wei","doi":"10.1186/s13567-024-01418-3","DOIUrl":"https://doi.org/10.1186/s13567-024-01418-3","url":null,"abstract":"Diarrhoea and preweaning mortality in piglets are crucial factors impacting the economic sustainability of the swine industry. Pathogenic infections are among the main causes of diarrhea and mortality. Group 3 innate lymphoid cells (ILC3s) are crucial for safeguarding against pathogenic infections. However, knowledge regarding the development and function of ILC3s in suckling piglets is currently limited. Our findings demonstrate that the development of ILC3s in suckling piglets gradually progresses from day 1 to day 21, with a notable increase observed on day 28. Additionally, the development of NKp46+ILC3s and the production of interleukin (IL)-17A by ILC3s displayed consistent patterns with the changes observed in ILC3s. Notably, interferon (IFN)-γ levels significantly increased on day 14. Moreover, the production of IFN-γ by NKp46+ILC3s was greater than that by NKp46-ILC3s. Importantly, when piglets were subjected to a 4-h challenge with enterotoxigenic Escherichia coli, both the percentages of ILC3s significantly increased, accompanied by increased IL-22 production, highlighting their importance in maintaining intestinal health. The outcomes of this study provide valuable insights for future related research.","PeriodicalId":23658,"journal":{"name":"Veterinary Research","volume":"55 1","pages":"159"},"PeriodicalIF":3.7,"publicationDate":"2024-12-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142855591","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Revealing novel and conservative CD8⁺T-cell epitopes with MHC B2 restriction on ALV-J.

IF 3.7 1区农林科学

Veterinary Research Pub Date : 2024-12-18 DOI: 10.1186/s13567-024-01426-3

Xueqing Li, Ziwei Li, Mulin Ma, Na Yang, Shanyao Du, Ming Liao, Manman Dai

{"title":"Revealing novel and conservative CD8+T-cell epitopes with MHC B2 restriction on ALV-J.","authors":"Xueqing Li, Ziwei Li, Mulin Ma, Na Yang, Shanyao Du, Ming Liao, Manman Dai","doi":"10.1186/s13567-024-01426-3","DOIUrl":"https://doi.org/10.1186/s13567-024-01426-3","url":null,"abstract":"MHC B2 haplotype chickens have been reported to induce strong immune response against various avian pathogens. However, little is known about the CD8+T-cell epitope with MHC B2-restricted on subgroup J avian leukosis virus (ALV-J). In this study, we explored the ALV-J-induced cellular immune response in B2 haplotype chickens in vivo. We found that ALV-J infection significantly increased the proportion of CD8+T cells in chickens and up-regulated the expression of cytotoxic genes like Granzyme A and antiviral genes like IFIT5 at 14 days post-infection (dpi). We selected 32 candidate peptides based on the peptide-binding motif and further identified three MHC B2-restricted CD8+T epitopes on ALV-J, including Pol652-660, Gag374-382, and Gag403-411 which induced significant levels of chicken IFN-γ production in splenocytes from ALV-J infected chickens using the ELISpot assay. In addition, we also verified that the three identified epitopes stimulated memory splenocytes elevating TNF-α and IL-2 protein expression. Importantly, we found that the three positive peptides were highly conserved among ALV-A, ALV-B, ALV-E, ALV-J, and ALV-K. Taken together, we identified three MHC B2-restricted CD8+T cell epitopes on ALV-J, providing a foundation for developing effective T cell epitope vaccines targeting conserved internal viral proteins.","PeriodicalId":23658,"journal":{"name":"Veterinary Research","volume":"55 1","pages":"164"},"PeriodicalIF":3.7,"publicationDate":"2024-12-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142855634","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Insights into genetic determinants of piglet survival during a PRRSV outbreak.

IF 3.7 1区农林科学

Veterinary Research Pub Date : 2024-12-18 DOI: 10.1186/s13567-024-01421-8

Joaquim Tarrés, Teodor Jové-Juncà, Carles Hernández-Banqué, Olga González-Rodríguez, Llilianne Ganges, Sofia Gol, Marta Díaz, Josep Reixach, Ramona N Pena, Raquel Quintanilla, Maria Ballester

{"title":"Insights into genetic determinants of piglet survival during a PRRSV outbreak.","authors":"Joaquim Tarrés, Teodor Jové-Juncà, Carles Hernández-Banqué, Olga González-Rodríguez, Llilianne Ganges, Sofia Gol, Marta Díaz, Josep Reixach, Ramona N Pena, Raquel Quintanilla, Maria Ballester","doi":"10.1186/s13567-024-01421-8","DOIUrl":"https://doi.org/10.1186/s13567-024-01421-8","url":null,"abstract":"Breeding animals to produce more robust and disease-resistant pig populations becomes a complementary strategy to the more conventional methods of biosecurity and vaccination. The objective of this study was to explore the ability of a panel of genetic markers and immunity parameters to predict the survival rates during a natural PRRSV outbreak. Ten-week-old female Duroc pigs (n = 129), obtained from 61 sows and 20 boars, were naturally infected with a highly pathogenic PRRSV genotype 1 strain. Prior to infection, piglets were screened for immunity parameters (IgG levels in plasma and SOX13 mRNA expression in blood) and genetic markers previously associated to PRRSV immune response and immunity traits. Additionally, the 20 boars were genotyped with a panel of 132 single nucleotide polymorphisms (SNPs). Survival analysis showed that mortality was significantly higher for animals with low basal IgG levels in plasma and/or high SOX13 mRNA expression in blood. The genotypes of sires for SNPs associated with IgG plasma levels, CRP in serum, percentage of γδ T cells, lymphocyte phagocytic capacity, total number of lymphocytes and leukocytes, and MCV and MCH were significantly associated with the number of surviving offspring. Furthermore, CD163 and GBP5 markers were also associated to piglet survival. The effects of these SNPs were polygenic and cumulative, survival decreased from 94 to 21% as more susceptible alleles were accumulated for the different markers. Our results confirmed the existence of genetic variability in survival after PRRSV infection and provided a set of genetic markers and immunity traits associated with PRRS resistance.","PeriodicalId":23658,"journal":{"name":"Veterinary Research","volume":"55 1","pages":"160"},"PeriodicalIF":3.7,"publicationDate":"2024-12-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142855631","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The F204S mutation in adrenodoxin oxidoreductase drives salinomycin resistance in Eimeria tenella.

IF 3.7 1区农林科学

Veterinary Research Pub Date : 2024-12-18 DOI: 10.1186/s13567-024-01431-6

Pei Sun, Chaoyue Wang, Fujie Xie, Linlin Chen, Yuanyuan Zhang, Xinming Tang, Dandan Hu, Yang Gao, Ning Zhang, Zhenkai Hao, Yonglan Yu, Jingxia Suo, Xun Suo, Xianyong Liu

{"title":"The F204S mutation in adrenodoxin oxidoreductase drives salinomycin resistance in Eimeria tenella.","authors":"Pei Sun, Chaoyue Wang, Fujie Xie, Linlin Chen, Yuanyuan Zhang, Xinming Tang, Dandan Hu, Yang Gao, Ning Zhang, Zhenkai Hao, Yonglan Yu, Jingxia Suo, Xun Suo, Xianyong Liu","doi":"10.1186/s13567-024-01431-6","DOIUrl":"https://doi.org/10.1186/s13567-024-01431-6","url":null,"abstract":"Salinomycin is a polyether ionophore widely used for the treatment of coccidiosis in poultry. However, the emergence of coccidia strains resistant to salinomycin presents challenges for control efforts, and the mechanisms underlying this resistance in Eimeria remain inadequately understood. In this study, 78 stable salinomycin-resistant strains were generated through experimental evolution approaches. Whole-genome sequencing of salinomycin-resistant Eimeria tenella isolates revealed single nucleotide polymorphisms (SNPs), with 12 candidate genes harboring nonsynonymous mutations identified. To confirm the candidate gene responsible for conferring salinomycin resistance, we leveraged reverse genetic strategies and identified a key amino acid substitution (F204S) in adrenodoxin oxidoreductase (EtADR), which markedly reduced susceptibility to salinomycin. Our results elucidate the complex interactions among salinomycin resistance, parasite fitness, point mutations, and the structure of EtADR, laying the foundation for future studies on drug resistance in Eimeria and contributing to the development of targeted control strategies.","PeriodicalId":23658,"journal":{"name":"Veterinary Research","volume":"55 1","pages":"170"},"PeriodicalIF":3.7,"publicationDate":"2024-12-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142855648","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Revealing novel CD8⁺ T-cell epitopes from the H5N1 avian influenza virus in HBW/B1 haplotype ducks.

IF 3.7 1区农林科学

Veterinary Research Pub Date : 2024-12-18 DOI: 10.1186/s13567-024-01415-6

Wanlin Jiao, Yingyi Chen, Zimin Xie, Li Zhao, Shanyao Du, Mulin Ma, Ming Liao, Manman Dai

{"title":"Revealing novel CD8+ T-cell epitopes from the H5N1 avian influenza virus in HBW/B1 haplotype ducks.","authors":"Wanlin Jiao, Yingyi Chen, Zimin Xie, Li Zhao, Shanyao Du, Mulin Ma, Ming Liao, Manman Dai","doi":"10.1186/s13567-024-01415-6","DOIUrl":"https://doi.org/10.1186/s13567-024-01415-6","url":null,"abstract":"The duck CD8+ T-cell response effectively defends against H5N1 highly pathogenic avian influenza virus (HPAIV) infection, but the recognized peptide is rarely identified. Here, we found that the ratio of CD8+ T cells and the expression of IFN-γ and cytotoxicity-associated genes, including granzyme A/K, perforin and IL2, at 7 days post-infection in peripheral blood mononuclear cells (PBMCs) from B1 haplotype ducks significantly increased in the context of defending against H5N1 AIV infection in vivo. Moreover, similar results were observed in cultured and sorted H5N1 AIV-stimulated duck CD8+ T cells in vitro. Next, we selected 109 epitopes as candidate epitopes on the basis of the MHC-I restriction binding peptide prediction website database and further identified twelve CD8+ T-cell epitopes that significantly increased IFN-γ gene expression after stimulating B1 haplotype duck memory PBMCs. In particular, NP338-346, NP473-481, M2-10, PB1540-548 and PA80-88 were highly conserved in H5N1, H5N6, H5N8, H7N9, and H9N2 AIVs. These findings provide directions for the development of universal T-cell epitope vaccines for AIV in ducks.","PeriodicalId":23658,"journal":{"name":"Veterinary Research","volume":"55 1","pages":"169"},"PeriodicalIF":3.7,"publicationDate":"2024-12-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142855644","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Heat shock protein 70 enhances viral replication by stabilizing Senecavirus A nonstructural proteins L and 3D.

IF 3.7 1区农林科学

Veterinary Research Pub Date : 2024-12-18 DOI: 10.1186/s13567-024-01414-7

Lei Hou, Penghui Zeng, Zhi Wu, Xiaoyu Yang, Jinshuo Guo, Yongyan Shi, Jiangwei Song, Jianwei Zhou, Jue Liu

{"title":"Heat shock protein 70 enhances viral replication by stabilizing Senecavirus A nonstructural proteins L and 3D.","authors":"Lei Hou, Penghui Zeng, Zhi Wu, Xiaoyu Yang, Jinshuo Guo, Yongyan Shi, Jiangwei Song, Jianwei Zhou, Jue Liu","doi":"10.1186/s13567-024-01414-7","DOIUrl":"https://doi.org/10.1186/s13567-024-01414-7","url":null,"abstract":"Senecavirus A (SVA) is an emerging pathogen that causes idiopathic vesicular infections in pig herds, posing a potential threat to their production performance. Heat shock protein 70 (Hsp70) is a molecular chaperone that plays an important role in host homeostasis under both physiological and stress conditions. However, the effects of Hsp70 on SVA infection and its underlying regulatory mechanisms remain unclear. Here, we confirmed that Hsp70 expression promotes SVA infection, as evidenced by the expression of viral proteins, viral titers, and the number of rSVA-eGFP-infected cells. This positive regulatory role of Hsp70 is mainly involved in post-entry stages of SVA. Viral proteins that interacted with Hsp70 were screened, and co-immunoprecipitation (co-IP) shows an interaction between Hsp70 and SVA L and 3D proteins. Subsequently, we determined that the expression of Hsp70 is beneficial for the stability of the SVA L and 3D proteins. Additionally, the substrate-binding domain (SBD) of Hsp70 plays an important role in the interaction between Hsp70 and SVA L or 3D proteins; and the deletion of this domain results in the loss of the stabilizing effect of Hsp70 on SVA L and 3D proteins and the positive regulatory effect of Hsp70 on SVA replication. These results reveal that Hsp70 promotes SVA infection by stabilizing viral L and 3D proteins and provides a strategy for preventing and controlling SVA infection.","PeriodicalId":23658,"journal":{"name":"Veterinary Research","volume":"55 1","pages":"158"},"PeriodicalIF":3.7,"publicationDate":"2024-12-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142855624","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Establishment of minigenomes for infectious bursal disease virus.

IF 3.7 1区农林科学

Veterinary Research Pub Date : 2024-12-18 DOI: 10.1186/s13567-024-01423-6

Hui Yang, Mingrui Zhang, Sanying Wang, Daxin Peng, Luis Martinez-Sobrido, Chengjin Ye

{"title":"Establishment of minigenomes for infectious bursal disease virus.","authors":"Hui Yang, Mingrui Zhang, Sanying Wang, Daxin Peng, Luis Martinez-Sobrido, Chengjin Ye","doi":"10.1186/s13567-024-01423-6","DOIUrl":"https://doi.org/10.1186/s13567-024-01423-6","url":null,"abstract":"Minigenomes (MGs) have greatly advanced research on the viral life cycle, including viral replication and transcription, virus‒host interactions, and the discovery of antivirals against RNA viruses. However, an MG for infectious bursal disease virus (IBDV) has not been well established. Here, we describe the development of IBDV MG, in which the entire coding sequences of viral genomic segments A and B are replaced with Renilla luciferase (Rluc) or enhanced green fluorescent protein (EGFP) reporter genes. Under the control of the RNA polymerase I promoter, the translation of IBDV MG is controlled by the viral proteins VP1 and VP3. Interestingly, IBDV B MG shows greater activity than does IBDV A MG. Moreover, the sense IBDV B MG was expressed at a higher level than the antisense IBDV B MG. In agreement with our previous findings, the translation of IBDV B MG controlled by VP1 and VP3 is independent of the cellular translation machinery components eukaryotic initiation factor (eIF)4E and eIF4G, but intact VP1 polymerase activity, VP3 dsRNA-binding activity, and the interaction between VP1 and VP3 are indispensable for both sense and antisense IBDV B MG activity. In addition, ribavirin, which inhibits IBDV replication, inhibits IBDV B MG activity in a dose-dependent manner. Collectively, the IBDV MG established in this study provides a powerful tool to investigate IBDV intracellular replication and transcription and virus‒host interactions and facilitates high-throughput screening for the identification of IBDV antivirals.","PeriodicalId":23658,"journal":{"name":"Veterinary Research","volume":"55 1","pages":"162"},"PeriodicalIF":3.7,"publicationDate":"2024-12-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142855594","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Identification of Brucella RS15060 as a novel type IV secretion system effector associated with bacterial virulence.

IF 3.7 1区农林科学

Veterinary Research Pub Date : 2024-12-18 DOI: 10.1186/s13567-024-01417-4

Yi Yin, Mingxing Tian, Guangdong Zhang, Hai Hu, Chan Ding, Shengqing Yu

{"title":"Identification of Brucella RS15060 as a novel type IV secretion system effector associated with bacterial virulence.","authors":"Yi Yin, Mingxing Tian, Guangdong Zhang, Hai Hu, Chan Ding, Shengqing Yu","doi":"10.1186/s13567-024-01417-4","DOIUrl":"https://doi.org/10.1186/s13567-024-01417-4","url":null,"abstract":"Brucella is an intracellular parasitic pathogen that causes the worldwide zoonotic disease brucellosis. The type IV secretion system (T4SS) is utilized to secrete various effectors to help Brucella form Brucella-containing vacuoles within the cell and accomplish intracellular trafficking and replication. Brucella has fewer recognized effector proteins than other intracellular parasites in the Proteobacteria, indicating that Brucella may contain a large number of unidentified effector proteins. In this study, the optimal conditions for inducing protein secretion from Brucella were screened, and the secreted proteins of 2308 and the T4SS-deficient mutant SV123 under optimal conditions were collected for comparative proteomics analysis. By label-free quantitative proteomics, we identified 15 differential proteins. Through the β-lactamase TEM1 assay and indirect immunofluorescence assay, we identified RS15060 and RS10635 as novel T4SS effectors. Furthermore, by constructing mutation strains and performing cell/mouse infection experiments, we found that deletion of the rs15060 gene reduced the capacity of Brucella to replicate in cells and cause chronic infection in mice. In conclusion, a novel Brucella T4SS effector protein, RS15060, was identified to be associated with virulence in this study, and the discovery of effector proteins is conducive to a more comprehensive elucidation of T4SS function as well as to uncovering the cryptic strategies of Brucella survival in cells.","PeriodicalId":23658,"journal":{"name":"Veterinary Research","volume":"55 1","pages":"168"},"PeriodicalIF":3.7,"publicationDate":"2024-12-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142855627","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Nucleotide-binding oligomerization domain 1 (NOD1) regulates microglial activation in pseudorabies virus infection.

IF 3.7 1区农林科学

Veterinary Research Pub Date : 2024-12-18 DOI: 10.1186/s13567-024-01416-5

Xiuxiu Sun, Xinxin Jin, Zhengdan Lin, Xi Liu, Junjie Yang, Li Li, Helong Feng, Wanpo Zhang, Changqin Gu, Xueying Hu, Xiaoli Liu, Guofu Cheng

{"title":"Nucleotide-binding oligomerization domain 1 (NOD1) regulates microglial activation in pseudorabies virus infection.","authors":"Xiuxiu Sun, Xinxin Jin, Zhengdan Lin, Xi Liu, Junjie Yang, Li Li, Helong Feng, Wanpo Zhang, Changqin Gu, Xueying Hu, Xiaoli Liu, Guofu Cheng","doi":"10.1186/s13567-024-01416-5","DOIUrl":"https://doi.org/10.1186/s13567-024-01416-5","url":null,"abstract":"The primary cause of viral encephalitis (VE) is invasion of the central nervous system (CNS) by the virus, which leads to neuroinflammation and poses a significant threat to global public health. Microglia, as CNS-resident macrophages, play a crucial role in neuroinflammation and are often identified as the preferred target for the prevention or treatment of VE. In this study, we used pseudorabies virus (PRV)-induced VE in mice and pigs as a model to investigate the regulation of microglial responses during viral encephalitis and explored the mechanism of microglial activation. Cellular experiments revealed that microglial activation was accompanied by cell migration, characteristic morphological changes, phagocytosis, inflammatory cytokine production, and antigen presentation. Transcriptome analysis revealed that genes related to inflammation in PRV-infected BV2 cells were significantly enriched. The expression of the NOD1 gene in BV2 cells was significantly increased during PRV infection, after which NOD1 in BV2 cells was silenced by siRNA and overexpressed via a plasmid. NOD1 was found to be involved in the secretion of cytokines in BV2 cells by regulating the MAPK/NF-κB signalling pathway. Mouse and pig experiments have shown that NOD1 is involved in the secretion of cytokines by microglia by regulating the MAPK/NF-κB signalling pathway during PRV infection.","PeriodicalId":23658,"journal":{"name":"Veterinary Research","volume":"55 1","pages":"161"},"PeriodicalIF":3.7,"publicationDate":"2024-12-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142855633","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0