Veterinary Research最新文献

{"title":"Assessing the efficiency of the bovine brucellosis surveillance-control system in a disease-free context through agent-based modelling.","authors":"Sofia Mlala, Sébastien Picault, Carole Sala, Pierre Villard, Jean-Luc Vinard, Viviane Hénaux","doi":"10.1186/s13567-025-01549-1","DOIUrl":"https://doi.org/10.1186/s13567-025-01549-1","url":null,"abstract":"<p><p>In France, bovine brucellosis is subject to stringent surveillance to ensure early detection of re-emergence and maintain the country's disease-free status. However, the cost-efficiency of such surveillance systems has rarely been assessed. This study aimed to: (1) evaluate the detection delay of bovine brucellosis for current and alternative surveillance systems following its reintroduction; and (2) estimate the associated surveillance and control costs. A mechanistic, stochastic, agent-based simulation model was developed to represent the spread of bovine brucellosis within and between cattle farms in mainland France after the introduction of an infected animal. The results showed: (1) improved efficiency (considering both effectiveness and cost) when suspending testing at introduction; (2) reduced efficiency when annually screening all adults in one-third of suckler herds compared to 20% of adults in all suckler herds; and (3) slightly improved efficiency when reporting abortion series instead of all individual cases. The current surveillance system detected infection within a median delay of 49-51 weeks, with a median of one infected farm at confirmation, regardless of the epidemiological context. For alternative systems, detection occurred within a median of 40-99 weeks, with one to three infected farms. These findings suggest that, under model assumptions, all surveillance systems maintained a relatively low number of infected herds. Our study provides cost-effectiveness information that will help stakeholders compare the efficiency of alternative scenarios, improve the current surveillance system and make relevant decisions about the allocations of resources.</p>","PeriodicalId":23658,"journal":{"name":"Veterinary Research","volume":"56 1","pages":"120"},"PeriodicalIF":3.7,"publicationDate":"2025-06-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144318110","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The pathogenesis of foot-and-mouth disease virus: current understandings and knowledge gaps.","authors":"Carolina Stenfeldt, Michael Eschbaumer, John Humphreys, Gisselle N Medina, Jonathan Arzt","doi":"10.1186/s13567-025-01545-5","DOIUrl":"10.1186/s13567-025-01545-5","url":null,"abstract":"<p><p>Foot-and-mouth disease (FMD) continues to be one of the most important diseases of livestock globally based upon both biological features and regulatory aspects. Few pathogens have had comparable impact on global livestock production and regulation of international trade in animal-derived products. The pathogenesis (interaction between pathogen and host) is central to the importance of the disease ranging from how the causal pathogen, FMD virus (FMDV), transmits between hosts and is maintained in populations. Key accomplishments over the last decade include description of the primary sites of infection in domestic species, delineating critical differences in temporo-anatomic progression in different host species and emphasizing that knowledge gained regarding FMDV pathogenesis in one host cannot necessarily be extrapolated and applied to a different host. Host responses to infection and viral genomics have been characterized with ever-increasing granularity. Yet, the numerous knowledge gaps that remain in understanding FMDV pathogenesis impede advancements in FMD control and eradication. For instance, it remains unclear if long-term asymptomatic FMDV carriers are biologically relevant (contagious) and the manner in which host genomics and transcriptomics affect pathogenesis during different phases of infection. The characterization of neoteric subclinical infection as a disease stage that is distinct from the persistent \"FMDV carrier state\" has emphasized the importance of sample collection from clinically unaffected animals for FMDV surveillance. Similarly, incorporating a phase of pre-clinical infectiousness in simulation modeling can dramatically improve prediction of FMD outbreaks in non-endemic regions. The outcome of FMDV infection with regards to viral persistence differs between host species as well as between individuals of the same species. Yet, we lack a satisfactory explanation of the host factors that drive the FMDV carrier state divergence. This review was based upon a gap-analysis workshop organized by the Global Foot-and-Mouth Disease Research Alliance (GFRA) in Buenos Aires, Argentina, in December of 2022. The purpose of this work is to summarize the current understanding of the distinct compartments of FMD pathogenesis with an emphasis on progress made within the last decade and present the critical knowledge gaps that continue to limit FMD control and eradication.</p>","PeriodicalId":23658,"journal":{"name":"Veterinary Research","volume":"56 1","pages":"119"},"PeriodicalIF":3.7,"publicationDate":"2025-06-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12172366/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144310505","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genetic characterization and pathogenicity analysis of three porcine epidemic diarrhea virus strains isolated from North China.","authors":"Ying Liu, Jinghui Fan, Wenyuan Gu, Yunhuan Zhao, Shuai Zhang, Yuzhu Zuo","doi":"10.1186/s13567-025-01554-4","DOIUrl":"10.1186/s13567-025-01554-4","url":null,"abstract":"<p><p>Porcine epidemic diarrhea (PED) is a highly contagious intestinal disease owing to porcine epidemic diarrhea virus (PEDV) infection. It is extremely detrimental to newborn piglets and has caused huge economic losses to the global pig industry. In this study, three PEDV strains of G2a PEDV-WF/2023, G2b PEDV-SX/2024 and PEDV-HS/2024 were successfully isolated from small intestine tissue samples with the analysis of their molecular structure characteristics, genetic characteristics and pathogenicity. Notably, these three PEDV strains had multiple unique aa mutations and extensive N-glycosylation in the D0 region, S1-NTD, COE epitope and SS6, respectively. Therefore, their structures were different compared to CV777 and PT-P5 strains. Furthermore, all the three PEDV strains caused severe clinical symptoms in 1-day-old piglets after infection. Among them, G2a PEDV-WF/2023 was the most detrimental to piglets, with highly levels of viral RNA in vivo. In contrast, PEDV-HS/2024 showed relatively weak pathogenicity to piglets, but it also caused the death of piglets. It might be attributed to the occurrence of individual mutations consistent with the amino acid sequence of G1b subtype in PEDV-HS/2024 strain. Findings in this study allow us to confirm that the G2a PEDV-WF/2023 strain is currently one of the most harmful epidemic strains to piglets. This study may benefit our understanding of the molecular structure characteristics, evolution trend and transmission dynamics of the epidemic strains in China. Moreover, it may provide potential reference for formulating more targeted PEDV vaccines, preventing and controlling this infection, and further curbing the cross-species spread of PEDV.</p>","PeriodicalId":23658,"journal":{"name":"Veterinary Research","volume":"56 1","pages":"118"},"PeriodicalIF":3.7,"publicationDate":"2025-06-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12166606/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144294984","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correction: Lumpy skin disease virus suppresses the antiviral response of bovine peripheral blood mononuclear cells that support viral dissemination.","authors":"Manoj Kumar, Ohad Frid, Asaf Sol, Alexander Rouvinski, Sharon Karniely","doi":"10.1186/s13567-025-01544-6","DOIUrl":"10.1186/s13567-025-01544-6","url":null,"abstract":"","PeriodicalId":23658,"journal":{"name":"Veterinary Research","volume":"56 1","pages":"117"},"PeriodicalIF":3.7,"publicationDate":"2025-06-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12164193/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144286623","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Highly suitable LFBK cells for African swine fever virus replication and type I interferon-induced immune studies.","authors":"Eun-Gyeong Lee, Sang-Min Kang, Dongseob Tark","doi":"10.1186/s13567-025-01543-7","DOIUrl":"10.1186/s13567-025-01543-7","url":null,"abstract":"<p><p>African swine fever virus (ASFV), the causative agent of African swine fever, is a fatal haemorrhagic virus affecting domestic pigs and wild boars. The primary target cells for ASFV infection are porcine alveolar macrophages (PAMs); however, PAM isolation and maintenance are technically challenging, and genetic manipulation of these cells is difficult. The lack of suitable cell lines that support ASFV infection and replication has significantly hindered vaccine development. This study aimed to overcome these limitations and advance ASFV research. The results demonstrate that the foetal porcine kidney cells (LFBK) are suitable for ASFV studies. We observed that ASFV replicated significantly more efficiently in LFBK cells than in PAMs. Furthermore, LFBK cells exhibited antiviral immune responses similar to PAMs following ASFV infection or DNA analog. These findings suggest that the LFBK cell line could provide a much-needed platform for studying ASFV replication and pathogenesis while serving as a valuable tool for understanding ASFV-induced immune mechanisms.</p>","PeriodicalId":23658,"journal":{"name":"Veterinary Research","volume":"56 1","pages":"116"},"PeriodicalIF":3.7,"publicationDate":"2025-06-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12153162/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144276039","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development and evaluation of an attenuated Avibacterium paragallinarum strain as a live vaccine candidate for infectious coryza.","authors":"Mengjiao Guo, Haonan Wang, Donghui Liu, Zongyi Bo, Chengcheng Zhang, Yantao Wu, Xiaorong Zhang","doi":"10.1186/s13567-025-01546-4","DOIUrl":"10.1186/s13567-025-01546-4","url":null,"abstract":"<p><p>Avibacterium paragallinarum (Av. paragallinarum), the causative agent of infectious coryza, is a significant pathogen responsible for substantial economic losses in the poultry industry. Current preventive strategies rely primarily on inactivated vaccines, which have limitations such as vaccine failure and limited cross-protection between serotypes. This study aimed to develop an attenuated strain of Av. paragallinarum as a potential live vaccine candidate. Using the Tn5-Kan transposon, we constructed a transposon mutant library and identified a mutant strain, designated 2019/HB64-40, which harbored a disrupted ksgA gene encoding a critical enzyme involved in ribosomal RNA methylation. Compared with the wild-type strain, the 2019/HB64-40 strain presented significantly reduced biofilm formation, lower hemagglutination titres, and impaired growth. Pathogenicity assessments in chickens demonstrated that the mutant strain displayed significantly attenuated virulence, characterized by fewer clinical symptoms and reduced bacterial shedding. Furthermore, following challenge, all unimmunized chickens presented severe clinical signs of infectious coryza at 2 dpi, with symptoms beginning to ameliorate by 5 dpi, culminating in a mean clinical sign score of 2.1. In contrast, only one chicken (1/10) in the immunized group displayed mild facial swelling and nasal discharge, with a mean clinical sign score of 0.1. The immunized group receiving the 2019/HB64-40 strain demonstrated 90% immunoprotection, highlighting the potential of this attenuated strain as a live vaccine candidate. While cross-serotype protection was not evaluated, the results suggest effective homologous protection and colonization capacity, underscoring its promising application in the prevention and treatment of infectious coryza.</p>","PeriodicalId":23658,"journal":{"name":"Veterinary Research","volume":"56 1","pages":"115"},"PeriodicalIF":3.7,"publicationDate":"2025-06-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12150455/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144258978","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"E627V mutation in PB2 protein promotes the mammalian adaptation of novel H10N3 avian influenza virus.","authors":"Meishan Song, Jianyu Liang, Sige Wang, Ruyi Gao, Xiaolong Lu, Wenhao Yang, Yu Chen, Jingxia Ma, Min Gu, Jiao Hu, Xiaowen Liu, Shunlin Hu, Xiaoquan Wang, Kaituo Liu, Xiufan Liu","doi":"10.1186/s13567-025-01534-8","DOIUrl":"10.1186/s13567-025-01534-8","url":null,"abstract":"<p><p>Since 2021, the novel H10N3 has caused four cases of human infection in China, the most recent of which occurred in December 2024, posing a potential threat to public health. Our previous studies indicated that several avian H10N3 strains are highly pathogenic in mice and can be transmitted between mammals via respiratory droplets without prior adaptation. By analyzing the genome sequence, we found that these H10N3 viruses carry the PB2-E627V mutation, which is becoming increasingly common in several subtypes of avian influenza viruses (AIV); however, its mechanism in mammalian adaptation remains unclear. Using a reverse genetics system, we investigated the role of PB2-E627V in the adaptation of H10N3 to mammals and poultry. Our findings demonstrate that the PB2-E627V mutation is critical for the high pathogenicity of novel H10N3 in mice and its ability to be transmitted through the air among mammals. Additionally, we found that the role of PB2-627 V in promoting AIV adaptation to mammals is comparable to that of PB2-627 K. More importantly, PB2-627 V appears to be equally suited to long-term persistence in poultry. Therefore, using PB2-627 V as a novel molecular marker to assess the epidemic potential of AIV is of great significance for preventing possible influenza pandemics in the future.</p>","PeriodicalId":23658,"journal":{"name":"Veterinary Research","volume":"56 1","pages":"111"},"PeriodicalIF":3.7,"publicationDate":"2025-06-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12145617/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144249782","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"PorV factor of the type IX secretion system and PosF porin act as adhesins in Riemerella anatipestifer infection.","authors":"Sen Li, Yanhua Wang, Congran Ning, Rongkun Yang, Yaxin Wu, Xu Cheng, Keke Ren, Minghua Huang, Xiong Liu, Naiji Zhou, Wanpo Zhang, Sishun Hu, Yuncai Xiao, Zili Li, Hongbo Zhou, Zhengfei Liu, Zutao Zhou","doi":"10.1186/s13567-025-01550-8","DOIUrl":"10.1186/s13567-025-01550-8","url":null,"abstract":"<p><p>Riemerella anatipestifer infection is a critical disease that is a major threat to the poultry industry worldwide. The adhesion and invasion of host cells are key steps in the primary stages of bacterial infection. However, the outer membrane proteins that mediate these events in R. anatipestifer are poorly characterized. In this study, the PorV and PosF proteins, as well as the previously described OMP71 protein, were identified as important mediators of the adhesion and invasion of duck embryo fibroblast (DEF) cells by R. anatipestifer. Affinity chromatography-based surface proteomics was used to screen for adhesion proteins. The surface proteins on DEF cells were labelled with biotin-avidin to enrich for outer membrane proteins of R. anatipestifer, which generated 11 candidate proteins that were tested further. Protein adhesion and blocking assays and polyclonal antiserum inhibition analysis revealed that the PorV, PosF, and OMP71 proteins are adhesion factors. Knockout of porV or posF reduced the adhesion and invasion of R. anatipestifer in DEF cells. Moreover, the pathogenicity of the mutant strains was significantly attenuated, which supports the hypothesis that PorV and PosF are important virulence factors required for the pathogenicity of R. anatipestifer. The PorV protein is a key component of the type IX secretory system and is responsible for transporting effector substrates to the extracellular environment, whereas PosF belongs to the porin superfamily of barrel-shaped transmembrane proteins. This is the first description that PorV is an adhesin involved in host‒microbial interactions, which represents a breakthrough in pathogenicity studies of R. anatipestifer and other members of Flavobacteriaceae.</p>","PeriodicalId":23658,"journal":{"name":"Veterinary Research","volume":"56 1","pages":"112"},"PeriodicalIF":3.7,"publicationDate":"2025-06-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12147357/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144249784","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Swine influenza A virus infection sets the local immunological landscape in subsequent infection with porcine reproductive and respiratory syndrome virus.","authors":"Janaïna Grevelinger, Olivier Bourry, Selma Schmidt, François Meurens, Céline Deblanc, Caroline Hervet, Aline Perrin, Stéphane Gorin, Mireille Le Dimna, Stéphane Quéguiner, Thibaut Larcher, Patricia Renson, Frédéric Paboeuf, Wilhelm Gerner, Nicolas Bertho, Gaëlle Simon","doi":"10.1186/s13567-025-01536-6","DOIUrl":"10.1186/s13567-025-01536-6","url":null,"abstract":"<p><p>Farmed pigs are frequently exposed to respiratory infections, with swine influenza A virus (swIAV) and porcine reproductive and respiratory syndrome virus (PRRSV) being key drivers. Most co-infection studies with these viruses have focused on PRRSV infection followed by swIAV. However, the reverse scenario, where swIAV is given first and then PRRSV, has not been explored. This infection sequence is plausible under natural conditions and warrants further study, especially given that influenza A virus has been shown in mice to impair alveolar macrophages, which are the target cells for PRRSV. This study aimed to evaluate the impact of swIAV infection on the alveolar macrophage population, clinical signs, immune responses, and viral loads during a secondary infection with PRRSV initiated 7 days after the initial swIAV exposure. Results demonstrated that primary swIAV infection did not exacerbate the clinical progression of PRRSV infection, nor did it result in significant differences in PRRSV loads or affect the alveolar macrophage population in the lungs of super-infected pigs as compared to those of pigs infected with PRRSV alone. However, swIAV pre-infection was associated with an increase in the number of conventional dendritic cells type 1 (cDC1), perforin-expressing T cells and NK-related lymphocytes in bronchoalveolar lavage. This coincided with an increase of PRRSV-specific IFN-γ producing CD4 T cells in blood detected 7 days post-PRRSV infection. These findings suggest that a swIAV infection could enhance immune responses during subsequent PRRSV infection by recruiting cDC1 and inducing IL-12, promoting a type-1 immune response, highlighting the complex interplay and often unexpected outcomes of viral co-infections occurring in close temporal proximity.</p>","PeriodicalId":23658,"journal":{"name":"Veterinary Research","volume":"56 1","pages":"114"},"PeriodicalIF":3.7,"publicationDate":"2025-06-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12147356/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144249785","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cytotoxicity induced by Aeromonas schubertii is orchestrated by a unique set of type III secretion system effectors.","authors":"Hana Michova, Jan Pliva, Anezka Jirsova, David Jurnecka, Jana Kamanova","doi":"10.1186/s13567-025-01548-2","DOIUrl":"10.1186/s13567-025-01548-2","url":null,"abstract":"<p><p>The type III secretion system (T3SS) is an important virulence factor of Gram-negative bacteria, including the genus Aeromonas, which represents a diverse group of aquatic bacteria. One member of the genus, Aeromonas schubertii, is an emerging pathogen in aquaculture, causing high mortality in snakehead fish. Infections are associated with the formation of white nodules in the internal organs, likely resulting from A. schubertii-induced apoptosis and/or necrosis. The present study investigates the type strain A. schubertii ATCC 43700, which encodes two distinct T3SSs located within Aeromonas pathogenicity islands 1 and 2, referred here to as API1 and API2. We analyzed their role in A. schubertii-induced cytotoxicity and identified novel T3SS effector proteins. Infections of HeLa cells revealed that API1, but not API2, mediates cytotoxicity and induces both apoptotic and necrotic cell death. Moreover, proteomic analysis identified seven candidate effectors secreted by the API1 injectisome. These included two previously described effectors, AopH and AopO from A. salmonicida, as well as five novel effectors named AopI, AopJ, AopL, AopT, and AopU, whose injection into host cells was validated using a split luciferase reporter system. Functional characterization showed that AopL, a homolog of Vibrio parahaemolyticus VopQ, induces caspase-3/-7-independent necrosis, while AopI, a homolog of ExoY from Pseudomonas aeruginosa, suppresses caspase-3/-7 activation and necrosis, revealing a pro-survival function. These results demonstrate the critical role of the API1 injectisome in A. schubertii-induced cytotoxicity and provide experimental identification of novel Aeromonas effectors that cooperate to fine-tune host cell cytotoxicity.</p>","PeriodicalId":23658,"journal":{"name":"Veterinary Research","volume":"56 1","pages":"113"},"PeriodicalIF":3.7,"publicationDate":"2025-06-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12147276/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144249781","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}