Veterinary Research最新文献

{"title":"Comparative genomic analysis of Mycoplasma agalactiae strain GM139 highlights unique surface architecture and pathogenic determinants.","authors":"Rohini Chopra-Dewasthaly, Katja Sommer, Maysa Santos Barbosa, Joachim Spergser","doi":"10.1186/s13567-025-01531-x","DOIUrl":"10.1186/s13567-025-01531-x","url":null,"abstract":"<p><p>Mycoplasma agalactiae causes one of the most serious forms of mycoplasmosis in small ruminants that is notifiable to the World Organization for Animal Health (WOAH). Possessing a plastic genome, its Vpma and other surface antigenic variations play important roles in its pathogenesis and systemic spread within the goat or sheep host, as well as its ability to jump to wild animals. The Vpma phenotypic profile of strain GM139 was recently compared to that of the type strain PG2, whereby GM139 predominantly exhibited stable expression of a single VpmaV protein in comparison with the high-frequency variable expression of all six Vpma proteins in PG2. The complete genome sequence of GM139 was generated, annotated for detailed analysis of the vpma locus and compared with the finished genomes of three distinct M. agalactiae strains (PG2, 5632, and GrTh01). Interestingly, GM139 presented a longer distinct vpma locus with ten genes, one of which is a chimera between the vpmaV and vpmaZ genes of PG2, which correlates very well with previous immunoblotting results and was confirmed here by nanoLC-MS/MS analysis; five vpmas are completely unique, whereas the other four share similarities with the vpmas of 5632, one of which is also partially homologous to vpmaZ_PG2. Additionally, features such as a larger spma locus, an intact gsmA known to encode a phase-variable glucan affecting serum resistance, and the presence of integrative and conjugative element (ICE) and transposases might have also influenced the pathogenicity and host range of these strains, segregating them into two well-separated phylogenetic clusters on the basis of a newly developed cgMLST scheme. This study highlights the plasticity and dynamic evolution of the M. agalactiae genome, especially its surface antigenic architecture.</p>","PeriodicalId":23658,"journal":{"name":"Veterinary Research","volume":"56 1","pages":"106"},"PeriodicalIF":3.7,"publicationDate":"2025-05-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12103780/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144143306","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Chicken interferon-induced transmembrane proteins inhibit Newcastle disease virus infection by affecting viral entry and W protein expression.","authors":"Jing Chen, Peiheng Li, Wancheng Zou, Ju Li, Yuhang Jiang, Letian Li, Pengfei Hao, Zihan Gao, Jiayi Hao, Xiaoshuang Shi, Chang Li","doi":"10.1186/s13567-025-01530-y","DOIUrl":"10.1186/s13567-025-01530-y","url":null,"abstract":"<p><p>Interferon-induced transmembrane proteins (IFITMs) are essential components of the innate immune system, demonstrating potent resistance to various enveloped viruses (such as influenza, West Nile, and dengue viruses) both in laboratory settings and in living organisms. Newcastle disease (ND), resulting from Newcastle disease virus (NDV), is a severe avian viral ailment with notable economic impact due to its significant mortality and morbidity rates. On the basis of the efficient antiviral effects of IFITMs, an in-depth study of the role and mechanism of NDV inhibition by chicken IFITMs (chIFITMs) is highly important for the prevention and control of this disease. In this study, we found that transient overexpression of chIFITMs effectively inhibited NDV (NDV Lasota, NDV Na) infection in DF-1 cells, with the highest inhibition rates of up to 89% and 99%, respectively, and that there was no significant difference in the antiviral effects of chIFITM1/2/3, which were not significantly different. Virus‒cell binding-entry assays revealed that chIFITMs restrict the entry process of NDV. Deleting endogenous chIFITMs enhances viral replication (more than 1.27-fold) and diminishes chIFNL3-mediated antiviral effects. Concurrently, overexpressing chIFITMs influences the expression level of the W protein; and co-immunoprecipitation experiments confirmed interaction between them. These findings suggest that the W protein could represent a novel target for the inhibition of NDV by chIFITMs. In summary, our results provide the initial comprehensive analysis of the antiviral effects of chIFITMs against NDV. This observation suggests that IFITMs are important barriers against zoonotic infections and important targets against viral invasion.</p>","PeriodicalId":23658,"journal":{"name":"Veterinary Research","volume":"56 1","pages":"104"},"PeriodicalIF":3.7,"publicationDate":"2025-05-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12093685/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144120909","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The dimeric conformation of PRRSV nsp1α is important for its ability to regulate viral RNA synthesis.","authors":"Qingyu Li, Jingbo Hu, Xue Jiao, Jing Shi, Chenxi Li, Yanhua Li","doi":"10.1186/s13567-025-01537-5","DOIUrl":"10.1186/s13567-025-01537-5","url":null,"abstract":"<p><p>PRRSV nsp1α, the first viral protein translated in virus-infected cells, is released from viral polyprotein 1a through autocleavage. It plays important roles in viral replication, the suppression of the host innate immune response, and the modulation of cell-mediated immune responses. Nsp1α forms a homodimer in vitro. In this study, we aimed to elucidate the functional significance of nsp1α dimerization. Using the alanine scanning strategy, we identified valine132 and proline134 as critical residues for nsp1α dimerization. Using recombinant viruses expressing an additional FLAG-nsp1α mutant (V132A or P134A), we demonstrated that both the V132A and P134A mutations disrupted nsp1α dimerization in PRRSV-infected cells. When ectopically expressed, the V132A or P134A mutation did not affect the ability of nsp1α to antagonize host type I IFN production or degrade SLA-I molecules. Introducing V132A or P134A mutations into an HP‒PRRSV replicon system significantly interfered with the expression of a Gaussia luciferase reporter and viral proteins, suggesting that nsp1α dimerization is critical for viral replication. Using PRRSV reverse genetics, a recombinant virus carrying the V132A mutation (vV132A) was successfully rescued, while the P134A mutation was lethal. Compared with the wild-type virus, vV132A significantly attenuated growth and reduced the relative expression levels of subgenomic RNAs in MARC-145 cells. In BHK-21 cells transfected with full-length cDNA clones, the P134A mutation nearly completely blocked the synthesis of specific sgRNAs at both the minus- and positive-strand levels while maintaining sgRNA6 accumulation. Thus, nsp1α dimerization is essential for viral RNA synthesis and transcriptional regulation but appears to be dispensable for both the autoproteolytic activity and immune evasion functions of PCPα. This study not only enhances our fundamental knowledge of PRRSV biology but also establishes a foundation for developing targeted antiviral strategies against PRRSV and related arteriviruses.</p>","PeriodicalId":23658,"journal":{"name":"Veterinary Research","volume":"56 1","pages":"105"},"PeriodicalIF":3.7,"publicationDate":"2025-05-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12096626/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144120910","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Detection, transmission and spread of airborne avian influenza and Newcastle disease viruses: experimental and field investigations.","authors":"Pierre Hostyn, Mieke Steensels, Bénédicte Lambrecht","doi":"10.1186/s13567-025-01533-9","DOIUrl":"10.1186/s13567-025-01533-9","url":null,"abstract":"<p><p>Both avian influenza (AI) and Newcastle disease (ND) viruses cause highly contagious respiratory diseases in chicken. These viruses are transmitted through the oro-faecal route, with airborne transmission via virus-laden droplets or dust. In this study, the Coriolis^® µ air sampler was evaluated for its suitability to assess the air detection and dispersion of highly pathogenic avian influenza virus (HPAIV) or live Newcastle disease virus (NDV) vaccines between chickens in both experimental and field settings. Experimental assays demonstrated HPAIV and NDV detection in air samples, indicating aerial persistence beyond the end of viral shedding measured in tracheal and cloacal swabs. Viral particles were detected in field air samples taken inside and outside HPAIV H5N1 outbreak farms, with outside aerial dispersion reaching up to 40 m from the exhaust fans. In accordance with these findings, viral particles were detected in air samples both indoors and outdoors from three live NDV-vaccinated farms; however, their aerial dispersion extended only up to 5 m from the exhaust fans. As observed in the NDV controlled assays, high levels of viral concentrations persisted in the air samples, whereas the viral concentrations in the individual swabs collected from the chickens were lower in the live NDV-vaccinated farms. For both the HPAIV and NDV field data, chicken density seemed to impact the viral air concentrations within and outside the studied farms. Coriolis^® µ proved effective as a non-invasive method for diagnosing AIV and NDV in both experimental and field studies, highlighting the value of air samples for monitoring poultry disease outbreaks.</p>","PeriodicalId":23658,"journal":{"name":"Veterinary Research","volume":"56 1","pages":"102"},"PeriodicalIF":3.7,"publicationDate":"2025-05-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12087104/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144102770","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Immunomodulatory effects of Eimeria maxima surface antigen (EmSAG) as an IFN-γ inhibitory molecule on peripheral blood mononuclear cells (PBMCs) and T cell subsets in chickens.","authors":"Xianglin Pu, Yiyuan Zhang, Xinmei Huang, Mingmin Lu, Lixin Xu, Ruofeng Yan, Xiangrui Li, Xiaokai Song","doi":"10.1186/s13567-025-01535-7","DOIUrl":"10.1186/s13567-025-01535-7","url":null,"abstract":"<p><p>Eimeria maxima (E. maxima) infection inhibits the expression of IFN-γ, a cytokine that is essential for the Th1 immune response and plays a key role in combating this parasite. In our preliminary investigations, we identified the E. maxima surface antigen (EmSAG) as an inhibitory molecule of IFN-γ. EmSAG was screened and characterised from an E. maxima sporozoite cDNA expression library. The present study aimed to evaluate the immunomodulatory effects of EmSAG on chicken peripheral blood mononuclear cells (PBMCs) and various T cell subsets. We analysed cell proliferation, nitric oxide (NO) release, and cytokine transcription. The results revealed that EmSAG boosts PBMC proliferation and promotes differentiation of CD4⁺/CD8⁺ T cells. Additionally, stimulation with EmSAG significantly inhibited NO release and IFN-γ transcription while enhancing the transcription of IL-4, IL-10, and TGF-β1 in chicken PBMCs. The sorting purity of T cell subsets was as follows: CD8⁺ (96.90%), CD4⁺ (86.25%), CD4⁺CD25^- (89.14%), and CD4⁺CD25⁺ regulatory T cells (Tregs; 92.16%). These purified subsets were co-incubated with EmSAG to analyse the transcription of hallmark cytokines associated with Th1, Th2, and Treg responses. EmSAG significantly inhibited the transcription of IFN-γ and IL-2 in both CD4⁺ and CD8⁺ T cells, while promoting the expression of IL-10, TGF-β1, and CTLA-4 in Tregs. Moreover, depletion of CD25⁺ cells reversed the EmSAG-induced suppression of IL-2 transcription and reduced its stimulating effects on IL-4 and IL-10 transcription in CD4⁺CD25^- T cells. These findings highlight the role of EmSAG as an inhibitor of IFN-γ, facilitating immune evasion by attenuating the Th1 immune response and modulating Treg cell function. This study provides critical insights into the immune evasion mechanisms utilised by chicken coccidia.</p>","PeriodicalId":23658,"journal":{"name":"Veterinary Research","volume":"56 1","pages":"103"},"PeriodicalIF":3.7,"publicationDate":"2025-05-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12090499/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144102771","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Review of respiratory syndromes in poultry: pathogens, prevention, and control measures.","authors":"Huixin Liu, Sijia Pan, Chenchen Wang, Wenwen Yang, Xiaofang Wei, Yang He, Ting Xu, Kaichuang Shi, Hongbin Si","doi":"10.1186/s13567-025-01506-y","DOIUrl":"10.1186/s13567-025-01506-y","url":null,"abstract":"<p><p>Respiratory syndromes (RS) include a variety of diseases that lead to respiratory dysfunction, resulting in significant economic losses for the poultry industry. Infectious agents and unfavourable environmental factors cause these respiratory diseases, and rapid transmission, high morbidity rates, and frequent mixed infections characterise them. The challenge in preventing and treating these diseases arises from the complexity of their triggers and the potential for secondary infections. Current vaccines often do not provide effective prevention, and the overuse of certain medications can lead to increased bacterial resistance, complicating prevention and control efforts. This review article examines the common sources of respiratory infections in poultry flocks, including infectious bronchitis virus, avian influenza virus, Newcastle disease virus, infectious laryngotracheitis virus, avian metapneumovirus, pathogenic Escherichia coli, Haemophilus paragallinarum, Mycoplasma gallisepticum, and Chlamydia. It also considers non-infectious factors such as adverse environmental conditions and management errors. The article provides an updated, comprehensive overview of widespread and economically significant poultry respiratory pathogens. It briefly discusses detection technology and vaccine development based on the transmission characteristics of RS. Furthermore, it explores prevention and control measures such as combination drug strategies and antibiotic alternatives to enhance understanding and implementation of effective disease prevention and control measures.</p>","PeriodicalId":23658,"journal":{"name":"Veterinary Research","volume":"56 1","pages":"101"},"PeriodicalIF":3.7,"publicationDate":"2025-05-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12085855/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144095325","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A novel chimpanzee adenovirus vector vaccine for protection against infectious bronchitis and Newcastle disease in chickens.","authors":"Chengyao Hou, Ruiqi Ni, Lijun Zhao, Wenjun Yan, Kailu Wang, Qinyuan Chu, Xinggui Chen, Hongning Wang, Xin Yang","doi":"10.1186/s13567-025-01528-6","DOIUrl":"10.1186/s13567-025-01528-6","url":null,"abstract":"<p><p>The development of effective poultry vaccines is crucial for maintaining flock health and productivity. In this study, we developed and evaluated a recombinant chimpanzee adenovirus vaccine (PAD-S1-HN) that simultaneously expresses the infectious bronchitis virus (IBV) spike subunit protein S1 and Newcastle disease virus (NDV) hemagglutinin-neuraminidase HN protein. The recombinant virus was successfully rescued in HEK293 cells, and transmission electron microscopy confirmed its typical adenoviral morphology. The expression of the IBV S1 and NDV HN proteins was validated by indirect immunofluorescence assay and western blotting. The vaccine demonstrated genetic stability over multiple passages and exhibited growth kinetics similar to those of the empty chimpanzee adenovirus vector. In animal trials, PAD-S1-HN effectively induced IBV- and NDV-specific antibodies, increased key cytokine levels, and stimulated mucosal immune responses, resulting in reduced viral loads, and alleviated clinical symptoms in vaccinated chickens. These findings indicate that the PAD-S1-HN vaccine provides strong immunogenicity and protective efficacy against IBV and NDV infections. Therefore, it presents a promising alternative to conventional vaccines, offering a novel approach for improving poultry disease management.</p>","PeriodicalId":23658,"journal":{"name":"Veterinary Research","volume":"56 1","pages":"100"},"PeriodicalIF":3.7,"publicationDate":"2025-05-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12083102/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144080780","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification of the methionine transporter MetQ in Streptococcus suis and its contribution to virulence and biofilm formation.","authors":"Camila Bosch, Carla García, Luis Saralegui, Lucille van Beek, Marien I de Jonge, Clara Marín, Jesús Arenas","doi":"10.1186/s13567-025-01522-y","DOIUrl":"https://doi.org/10.1186/s13567-025-01522-y","url":null,"abstract":"<p><p>Streptococcus suis is a Gram-positive bacterium responsible for various infections in both pigs and humans. This study investigates the role of methionine acquisition in the growth and virulence of S. suis. The putative methionine transport system is organised as an operon comprising the metQ gene and genes encoding a transposase and an ATPase, forming a typical tripartite ABC transporter. This operon is conserved across multiple streptococcal species, including both animal and human pathogens. We examined whether MetQ functions as a methionine-binding protein and its role in bacterial infection. Using Western blotting and flow cytometry with a specific antiserum, we demonstrated that MetQ is produced in vitro by the S. suis reference strain P1/7 under methionine-limited conditions and is located on the bacterial cell surface. Growth assays in chemically defined media revealed that a metQ deletion mutant (P1/7∆metQ) exhibited impaired growth under methionine-restricted conditions but grew normally in a nutrient-rich medium, suggesting that MetQ primarily transports methionine. Isothermal Titration Calorimetry demonstrated that MetQ binds L-methionine with a dissociation constant (K_D) of 7.1 µM. In a murine infection model, the metQ mutant showed reduced dissemination to internal organs compared to the wild type. Furthermore, the mutant showed decreased intracellular survival in murine macrophages and increased sensitivity to oxidative stress, while exhibited enhanced biofilm formation compared to the wild type. Our findings indicate that MetQ is essential for methionine uptake under methionine-restricted conditions, which is critical for bacterial nutrition, immune evasion, and pathogenicity during infection.</p>","PeriodicalId":23658,"journal":{"name":"Veterinary Research","volume":"56 1","pages":"99"},"PeriodicalIF":3.7,"publicationDate":"2025-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12063423/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144012572","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ingestion of Artemisia argyit essential oil combats Salmonella pullorum infections by altering gut microbiota composition in chicks.","authors":"Linlin Ding, Kaige Qi, Yutong Zhou, Qingjie Li, Minda Liu, Na Hu, Jianfeng Wang, Jiazhang Qiu, Xuming Deng, Lei Xu","doi":"10.1186/s13567-025-01527-7","DOIUrl":"https://doi.org/10.1186/s13567-025-01527-7","url":null,"abstract":"<p><p>Pullorum disease, caused by Salmonella pullorum (S. pullorum), is a highly contagious illness affecting the poultry industry. Emerging evidence suggests that Artemisia argyit essential oil can influence the composition of gut microbes in the host, thereby promoting overall health. However, the specific mechanisms by which Artemisia argyit essential oil modulates gut microbiota to combat S. pullorum infection remains unclear. This study explored the effectiveness of various doses of Artemisia argyit essential oil in preventing S. pullorum infection in chicks. Our findings indicate that consuming this essential oil can mitigate the intestinal mucosal barrier damage and excessive inflammatory response caused by S. pullorum, as well as reverse the weight loss seen in infected chicks. Additionally, chicks that received faecal microbiota transplantation (FMT) from the gut microbiota of Artemisia argyit essential oil donors exhibited notable recovery from S. pullorum infections. This suggests that the observed protection may be linked to the modulation of gut microbiota. Furthermore, 16S rRNA sequencing revealed an increased abundance of Lactobacillus reuteri (L. reuteri), which along with the activation of Wnt/β-catenin pathways, played critical roles in the enhanced health of S. pullorum-infected chicks treated with Artemisia argyit essential oil. In summary, these findings highlight that the dietary inclusion of Artemisia argyit essential oil promotes the intestinal enrichment of L. reuteri, offering a promising strategy for the treatment and prevention of pullorum disease in chicks.</p>","PeriodicalId":23658,"journal":{"name":"Veterinary Research","volume":"56 1","pages":"98"},"PeriodicalIF":3.7,"publicationDate":"2025-05-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12057167/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144001353","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"SIRT2 inhibition enhances mitochondrial apoptosis in Brucella-infected bovine placental trophoblast cells.","authors":"Mengyu Zhang, Lin Qi, Junmei Li, NingQiu Yuan, Yunyi Zhai, Mingyue Hao, Dong Zhou, Wei Liu, Yaping Jin, Aihua Wang","doi":"10.1186/s13567-025-01518-8","DOIUrl":"https://doi.org/10.1186/s13567-025-01518-8","url":null,"abstract":"<p><p>Brucella is a successful pathogen that employs a plethora of immune evasion mechanisms. This contributes to pathogenesis and persistence and limits the efficacy of available treatments. An increasing understanding of host‒pathogen interactions suggests that integrating host-directed strategies with existing anti-Brucella treatments could lead to more effective bacterial clearance and a reduction in drug-resistant strains. SIRT2 is a nicotinamide adenine dinucleotide (NAD⁺)-dependent deacetylase found in mammals. It can deacetylate various transcription factors and regulatory proteins, playing crucial roles in host‒pathogen interactions and pathogen infection-induced apoptosis. In this study, we investigated the role of SIRT2 in Brucella-induced cell apoptosis using bovine placental trophoblast cells. Our results indicate that B. abortus A19 infection upregulates SIRT2 protein expression and significantly induces mitochondrial apoptosis in these cells. Furthermore, inhibition of SIRT2 exacerbates B. abortus A19-induced mitochondrial apoptosis and markedly inhibits intracellular bacterial survival. These results prove the role of SIRT2 in Brucella pathogenesis and the mechanism of action.</p>","PeriodicalId":23658,"journal":{"name":"Veterinary Research","volume":"56 1","pages":"97"},"PeriodicalIF":3.7,"publicationDate":"2025-05-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12049057/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144017859","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}