Veterinary Research最新文献

Correction: Blastocystis occurrence and subtype diversity in European wild boar (Sus scrofa) from the Iberian Peninsula. 更正：伊比利亚半岛欧洲野猪（Sus scrofa）中布氏囊虫的发生和亚型多样性。

IF 3.7 1区农林科学

Veterinary Research Pub Date : 2024-11-14 DOI: 10.1186/s13567-024-01408-5

Pamela C Köster, Ana M Figueiredo, Jenny G Maloney, Alejandro Dashti, Begoña Bailo, Rita T Torres, Carlos Fonseca, Atle Mysterud, Miguel Á Habela, Antonio Rivero-Juarez, Joaquín Vicente, Emmanuel Serrano, Maria C Arnal, Daniel Fernández de Luco, José A Armenteros, Ana Balseiro, Guillermo A Cardona, João Carvalho, Dário Hipólito, Joana Fernandes, Josman D Palmeira, Rafael Calero-Bernal, David González-Barrio, Monica Santin, David Carmena

引用次数: 0

Porcine reproductive and respiratory syndrome virus degrades TANK-binding kinase 1 via chaperon-mediated autophagy to suppress type I interferon production and facilitate viral proliferation. 猪繁殖与呼吸综合征病毒通过伴侣介导的自噬作用降解 TANK 结合激酶 1，从而抑制 I 型干扰素的产生并促进病毒增殖。

IF 3.7 1区农林科学

Veterinary Research Pub Date : 2024-11-14 DOI: 10.1186/s13567-024-01392-w

Shuang-Shuang Zhao, Qisheng Qian, Yao Wang, Songlin Qiao, Rui Li

{"title":"Porcine reproductive and respiratory syndrome virus degrades TANK-binding kinase 1 via chaperon-mediated autophagy to suppress type I interferon production and facilitate viral proliferation.","authors":"Shuang-Shuang Zhao, Qisheng Qian, Yao Wang, Songlin Qiao, Rui Li","doi":"10.1186/s13567-024-01392-w","DOIUrl":"10.1186/s13567-024-01392-w","url":null,"abstract":"Porcine reproductive and respiratory syndrome virus (PRRSV) has led to significant economic losses in the global swine industry. Type I interferon (IFN-I) plays a crucial role in the host's resistance to PRRSV infection. Despite extensive research showing that PRRSV employs multiple strategies to antagonise IFN-I induction, the underlying mechanisms remain to be fully elucidated. In this study, we have discovered that PRRSV inhibits the production of IFN-I by degrading TANK-binding kinase 1 (TBK1) through chaperon-mediated autophagy (CMA). From a mechanistic standpoint, PRRSV nonstructural protein 2 (Nsp2) increases the interaction between the heat shock protein member 8 (HSPA8) and TBK1. This interaction leads to the translocation of TBK1 into lysosomes for degradation, mediated by lysosomal-associated membrane protein 2A (LAMP2A). As a result, the downstream activation of IFN regulatory factor 3 (IRF3) and the production of IFN-I are hindered. Together, these results reveal a new mechanism by which PRRSV suppresses host innate immunity and contribute to the development of new antiviral strategies against the virus.","PeriodicalId":23658,"journal":{"name":"Veterinary Research","volume":"55 1","pages":"151"},"PeriodicalIF":3.7,"publicationDate":"2024-11-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11566183/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142629476","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bactericidal activities and biochemical features of 16 antimicrobial peptides against bovine-mastitis causative pathogens. 16 种抗菌肽对牛乳腺炎致病菌的杀菌活性和生化特征。

IF 3.7 1区农林科学

Veterinary Research Pub Date : 2024-11-14 DOI: 10.1186/s13567-024-01402-x

Hye-Sun Cho, Dohun Kim, Hyoim Jeon, Prathap Somasundaram, Nagasundarapandian Soundrarajan, Chankyu Park

{"title":"Bactericidal activities and biochemical features of 16 antimicrobial peptides against bovine-mastitis causative pathogens.","authors":"Hye-Sun Cho, Dohun Kim, Hyoim Jeon, Prathap Somasundaram, Nagasundarapandian Soundrarajan, Chankyu Park","doi":"10.1186/s13567-024-01402-x","DOIUrl":"10.1186/s13567-024-01402-x","url":null,"abstract":"Mastitis, often caused by bacterial infection, is an inflammatory condition affecting the mammary glands. The condition is particularly prevalent in dairy cattle. Current treatment of bovine mastitis heavily relies on the use of antibiotics. To identify alternative solutions to antibiotic use, we evaluated the antimicrobial activity of 14 cathelicidins reported from 10 animal species. In conjunction, we assessed two bacteriocins against the bovine-mastitis causative bacterial panel, consisting of Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus, Bacillus cereus, Enterococcus faecalis, Streptococcus agalactiae, Streptococcus dysgalactiae, and Streptococcus equi. Among the antimicrobial peptides (AMPs), cc-CATH3, ML-CATH, and PD-CATH proved to be highly active (minimum inhibitory concentration of 2-41 μg/mL, 0.2-10.3 μM) against all bacterial strains in the panel and field isolates from milk, with elevated somatic cell counts (≥ 500,000 cells/mL). Of the AMPs tested in this study, ML-CATH presented the highest level of effectiveness in controlling mastitis-associated bacterial strains while also possessing minimal cytotoxicity and functional stability against pH change and a high salt condition. The results of in silico analyses on the biochemical features of 12 helical cathelicidins revealed that the charge of AMPs appears to be a major determinant in killing Gram-negative bacteria. Furthermore, we observed a unique motif, \"N(n≥3)-P(n≥1)-N(n≥3)\", from the sequences of PMAP-36, cc-CATH3, ML-CATH, and PD-CATH that exhibits potent antimicrobial activity against a broad spectrum of bacteria compared to others. Our findings support the proposition that AMPs could serve as effective antimicrobial alternatives to conventional antibiotics in treating complex animal diseases caused by microbial infection, such as bovine mastitis.","PeriodicalId":23658,"journal":{"name":"Veterinary Research","volume":"55 1","pages":"150"},"PeriodicalIF":3.7,"publicationDate":"2024-11-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11566078/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142629473","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

TRIM28 regulates the coagulation cascade inhibited by p72 of African swine fever virus. TRIM28调节非洲猪瘟病毒p72抑制的凝血级联。

IF 3.7 1区农林科学

Veterinary Research Pub Date : 2024-11-12 DOI: 10.1186/s13567-024-01407-6

Xuejiao Zhu, Fang Li, Baochao Fan, Yongxiang Zhao, Junming Zhou, Dandan Wang, Renqiang Liu, Dongming Zhao, Huiying Fan, Bin Li

{"title":"TRIM28 regulates the coagulation cascade inhibited by p72 of African swine fever virus.","authors":"Xuejiao Zhu, Fang Li, Baochao Fan, Yongxiang Zhao, Junming Zhou, Dandan Wang, Renqiang Liu, Dongming Zhao, Huiying Fan, Bin Li","doi":"10.1186/s13567-024-01407-6","DOIUrl":"10.1186/s13567-024-01407-6","url":null,"abstract":"In 2018, African swine fever virus (ASFV) emerged in China, causing extremely serious economic losses to the domestic pig industry. Infection with ASFV can cause disseminated coagulation, leading to the consumption of platelets and coagulation factors and severe bleeding. However, the mechanism of virus-induced coagulation has yet to be established. In our study, ASFV downregulated the coagulation process, as detected by D-dimer (D2D) and Factor X (F10) expression in pigs challenged with ASFV HLJ/18. In vitro, ASFV infection increased Factor IX (F9) and Factor XII (F12) expression while downregulating F10 expression in porcine alveolar macrophages (PAMs). African swine fever virus induced both intrinsic and extrinsic coagulation cascades. In addition, several encoded proteins affect the expression of the crucial coagulation protein F10, and among the encoded proteins, p72 inhibits the activity and expression of F10. Proteomic analysis also revealed that p72 is involved in the coagulation cascade. p72 can interact with F10, and its inhibitory functional domains include amino acids 423-432 and amino acids 443-452. Finally, we found that F10 and p72 interact with tripartite motif-containing protein 28 (TRIM28). TRIM28 knockdown resulted in a decrease in F10 expression. Importantly, TRIM28 contributes to the reduction in F10 protein expression regulated by p72. Our findings revealed an inhibitory effect of the viral protein p72 on the ASFV infection-induced coagulation cascade and revealed a role of TRIM28 in reducing F10 expression, revealing a molecular mechanism of ASFV-associated coagulation.","PeriodicalId":23658,"journal":{"name":"Veterinary Research","volume":"55 1","pages":"149"},"PeriodicalIF":3.7,"publicationDate":"2024-11-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11559047/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142627888","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

capD deletion in the Elizabethkingia miricola capsular locus leads to capsule production deficiency and reduced virulence. 伊丽莎白金线虫蒴果基因座中的 capD 缺失会导致蒴果生成不足和毒力降低。

IF 3.7 1区农林科学

Veterinary Research Pub Date : 2024-11-11 DOI: 10.1186/s13567-024-01394-8

Ruixue Hu, Fangyuan Liu, Fang Yu, Jiahao Hou, Dan Chen, Zemao Gu

{"title":"capD deletion in the Elizabethkingia miricola capsular locus leads to capsule production deficiency and reduced virulence.","authors":"Ruixue Hu, Fangyuan Liu, Fang Yu, Jiahao Hou, Dan Chen, Zemao Gu","doi":"10.1186/s13567-024-01394-8","DOIUrl":"10.1186/s13567-024-01394-8","url":null,"abstract":"Elizabethkingia miricola is a multidrug-resistant pathogen that can cause life-threatening infections in immunocompromised humans and outbreaks in amphibians. However, the specific virulence factors of this microorganism have not been described. In this study, we identified the polysaccharide biosynthesis protein-encoding gene capD, which is located in the conserved region of the Wzy-dependent capsule synthesis gene cluster in the E. miricola strain FL160902, and investigated its role in the pathogenesis of E. miricola. Our results revealed that the capD deletion strain (ΔcapD) lost its typical encapsulated structure, with a 45% reduction in cell wall thickness. CapD affects wza expression in the capsule polysaccharide synthesis pathway. Furthermore, the survival rates were significantly reduced in ΔcapD in response to complement-mediated killing, desiccation stress, and macrophage phagocytosis, whereas biofilm formation, surface hydrophobicity, and adherence to both endothelial and epithelial cells were increased. Additionally, the deletion of capD sharply attenuated the virulence of E. miricola in a frog infection model. Complementation of the capD gene restored the biological properties and virulence to wild-type levels. Overall, these findings suggest that CapD contributes to polysaccharide synthesis and plays a crucial role in the pathogenesis of E. miricola.","PeriodicalId":23658,"journal":{"name":"Veterinary Research","volume":"55 1","pages":"148"},"PeriodicalIF":3.7,"publicationDate":"2024-11-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11552330/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142629474","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The introduction of a highly virulent PRRSV strain in pig farms is associated with a change in the pattern of influenza A virus infection in nurseries. 猪场引入高致病性 PRRSV 株系与保育舍甲型流感病毒感染模式的改变有关。

IF 3.7 1区农林科学

Veterinary Research Pub Date : 2024-11-09 DOI: 10.1186/s13567-024-01406-7

Ivan Domingo-Carreño, Maria Soledad Serena, Gerard Eduard Martín-Valls, Hepzibar Clilverd, Laia Aguirre, Martí Cortey, Enric Mateu

{"title":"The introduction of a highly virulent PRRSV strain in pig farms is associated with a change in the pattern of influenza A virus infection in nurseries.","authors":"Ivan Domingo-Carreño, Maria Soledad Serena, Gerard Eduard Martín-Valls, Hepzibar Clilverd, Laia Aguirre, Martí Cortey, Enric Mateu","doi":"10.1186/s13567-024-01406-7","DOIUrl":"10.1186/s13567-024-01406-7","url":null,"abstract":"The present study aimed to determine the dynamics of influenza A virus (IAV) infection in two endemically infected farms (F1 and F2), where a longitudinal follow-up of piglets was performed from birth to 8-12 weeks of age. During the study, a highly virulent isolate of porcine reproductive and respiratory syndrome virus (PRRSV) was introduced on both farms. This allowed us to examine the impact of such introduction on the patterns of infection, disease, and the antibody response of pigs to IAV infection. The introduction of the new PRRSV strain coincided with a change in the dynamics of IAV infection on both farms. In F1, the cumulative incidence of IAV increased from 20% before the outbreak to 67.5%, together with the existence of animals that tested positive for IAV (RT‒qPCR) in nasal swabs for two or more consecutive samples. In F2, the cumulative incidence of IAV increased from 50% before the PRRSV outbreak to 70%, and the proportion of prolonged IAV shedders increased sharply. Additionally, some animals were infected with the same IAV twice during the observation period. In contrast to previous reports, our study revealed that prolonged shedding was not related to the titres of maternally derived antibodies at the time of infection but was significantly (p < 0.05) related to PRRSV infection status. Notably, both before and after the PRRSV outbreak, a high proportion of IAV-infected piglets did not seroconvert, which was significantly (p < 0.05) related to the hemagglutination inhibition titres against IAV when infected.","PeriodicalId":23658,"journal":{"name":"Veterinary Research","volume":"55 1","pages":"147"},"PeriodicalIF":3.7,"publicationDate":"2024-11-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11549838/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142627487","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Retrospective investigation of porcine circoviruses in cases of porcine dermatitis and nephropathy syndrome. 猪皮炎和肾病综合征病例中猪圆环病毒的回顾性调查。

IF 3.7 1区农林科学

Veterinary Research Pub Date : 2024-11-09 DOI: 10.1186/s13567-024-01405-8

Àlex Cobos, Mariano Domingo, Mónica Pérez, Eva Huerta, Anna Llorens, Joaquim Segalés, Marina Sibila

{"title":"Retrospective investigation of porcine circoviruses in cases of porcine dermatitis and nephropathy syndrome.","authors":"Àlex Cobos, Mariano Domingo, Mónica Pérez, Eva Huerta, Anna Llorens, Joaquim Segalés, Marina Sibila","doi":"10.1186/s13567-024-01405-8","DOIUrl":"10.1186/s13567-024-01405-8","url":null,"abstract":"Porcine dermatitis and nephropathy syndrome (PDNS) is a severe condition that affects mainly growing pigs and is considered to be caused by a type III hypersensitivity reaction. Although porcine circovirus 2 (PCV-2) is the antigen linked to this condition, porcine circovirus 3 (PCV-3) has also been proposed to be causally associated with PDNS. Moreover, the initial description of porcine circovirus 4 (PCV-4) also related this novel agent to this clinicopathological entity. Therefore, this retrospective study included a large number of PDNS cases (n = 102) fulfilling specific histologic criteria in search of known porcine circoviruses (PCV-1 to PCV-4) by conventional and/or quantitative PCR (qPCR). All the samples were subjected to PCV-2 immunohistochemistry (IHC) or conventional in situ hybridization (C-ISH), and RNAscope® (R-ISH) was used to study PCV-2 and PCV-3 localization in a subset of the samples. All PDNS cases were PCV-2 positive by qPCR, while 30 of them (29.4%) yielded PCV-3 qPCR positivity; PCV-2 viral loads were significantly greater than PCV-3 viral loads. All animals were negative for PCV-1 and PCV-4. By C-ISH/IHC, 63 cases (61.8%) were positive for PCV-2, with low to moderate amounts of antigen. R-ISH demonstrated higher sensitivity, as all studied cases were positive; however, neither PCV-2 nor PCV-3 were consistently found within characteristic PDNS lesions. These results indicate that all PDNS-affected pigs were infected with PCV-2, emphasizing the likelihood that this viral antigen is causally linked to this condition. In contrast, no evidence of the association of PCV-1, PCV-3 or PCV-4 with PDNS was found.","PeriodicalId":23658,"journal":{"name":"Veterinary Research","volume":"55 1","pages":"146"},"PeriodicalIF":3.7,"publicationDate":"2024-11-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11549871/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142629477","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A panoramic view of the molecular epidemiology, evolution, and cross-species transmission of rosaviruses. 罗沙病毒的分子流行病学、进化和跨物种传播全景。

IF 3.7 1区农林科学

Veterinary Research Pub Date : 2024-11-08 DOI: 10.1186/s13567-024-01399-3

Minyi Zhang, Shunchang Fan, Minyi Liang, Ruojun Wu, Jingli Tian, Juxian Xian, Xiaofeng Zhou, Qing Chen

{"title":"A panoramic view of the molecular epidemiology, evolution, and cross-species transmission of rosaviruses.","authors":"Minyi Zhang, Shunchang Fan, Minyi Liang, Ruojun Wu, Jingli Tian, Juxian Xian, Xiaofeng Zhou, Qing Chen","doi":"10.1186/s13567-024-01399-3","DOIUrl":"10.1186/s13567-024-01399-3","url":null,"abstract":"Rosavirus is a newly discovered member of the family Picornaviridae that was initially detected in wild rodents and subsequently in children with diarrhoea. Nevertheless, there is a significant gap in our understanding of the geographical distribution, phylogenetic relationships, evolutionary patterns, and transmission of rosaviruses. To address these issues, we analysed 434 rodents and shrews from five different species that were collected in southern China. Using PCR screening of faecal samples, we detected rosaviruses in Norway rats (Rattus norvegicus) and identified two previously undocumented host species: tanezumi rats (Rattus tanezumi) and Asian house shrews (Suncus murinus). Rosaviruses were particularly common in these animals, with an overall prevalence rate of 32.49% (141/434). For genetic and evolutionary analyses, we selected six representative positive samples to amplify the complete genomes of rosaviruses. Bayesian phylogenetic analysis suggested that our sequences clustered within the genus Rosavirus, where genotype B sequences are the closest relatives. The elevated nonsynonymous-to-synonymous ratios observed in rosavirus B may be attributed to relaxed selection pressures driven by virus spillover events. On the basis of the available data, it is hypothesized that the genus Rosavirus may have originated from Norway rats around the year 1339. In summary, these findings provide valuable insights into the complex evolutionary history of rosaviruses and underscore the urgent need for ongoing surveillance of this virus.","PeriodicalId":23658,"journal":{"name":"Veterinary Research","volume":"55 1","pages":"145"},"PeriodicalIF":3.7,"publicationDate":"2024-11-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11545274/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142629472","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Protective efficacy of a recombinant adenovirus expressing novel dual F and HN proteins of bovine parainfluenza virus type 3. 表达新型 F 和 HN 双蛋白的重组腺病毒对牛副流感病毒 3 型的保护作用。

IF 4.3 1区农林科学

Veterinary Research Pub Date : 2024-11-07 DOI: 10.1186/s13567-024-01400-z

Jiaqi Zhang, Jinbo Wu, Qing Zhu, Xiangyue Huang, Zhaohui Zhang, Chenxi Zhu, Gunan Deng, Ajia Ake, Yuanzhen Ma, Chunsai He, Rui Guo, Hua Yue, Lan Lan, Bin Zhang

{"title":"Protective efficacy of a recombinant adenovirus expressing novel dual F and HN proteins of bovine parainfluenza virus type 3.","authors":"Jiaqi Zhang, Jinbo Wu, Qing Zhu, Xiangyue Huang, Zhaohui Zhang, Chenxi Zhu, Gunan Deng, Ajia Ake, Yuanzhen Ma, Chunsai He, Rui Guo, Hua Yue, Lan Lan, Bin Zhang","doi":"10.1186/s13567-024-01400-z","DOIUrl":"10.1186/s13567-024-01400-z","url":null,"abstract":"Bovine parainfluenza virus type 3 (BPIV3) is a viral respiratory pathogen that infects cattle and causes significant economic losses. We generated a recombinant adenovirus called rHAd5-F + HN by expressing the fusion (F) and hemagglutinin-neuraminidase (HN) glycoprotein of BPIV3 using the human adenovirus serotype 5 (rHAd5). We evaluated its effects on humoral and cellular immune responses in mice (n = 45) and calves (n = 9). Serum antibody responses were assessed by enzyme-linked immunosorbent assay (ELISA), hemagglutination inhibition (HI), and neutralising antibodies (NAb). After boosting immunity with rHAd5-F + HN, mice produced significantly higher levels of antibodies against the BPIV3 genotype A and genotype C strains. The production of antibodies exceeded those produced by adenoviruses rHAd5-F and rHAd5-HN, which express the F and HN glycoprotein, respectively. The percentages of splenic CD3+/CD8+T lymphocytes and IL-4+ cytokines in rHAd5-F + HN mice were considerably higher than those in the control group. Mice immunised with rHAd5-F + HN exhibited much lower viral loads in the lungs and tracheas compared to the control group. Additionally, the lungs of mice vaccinated with rHAd5-F + HN showed no notable histopathological changes. On the other hand, rHAd5-F + HN produced a humoral immune response in calves. Following the booster intramuscular injection with the rHAd5-F + HN, the serum antibody levels against BPIV3 genotype C strain were 1:20 452, 1:1024, and 1:426 in calves, as detected by ELISA, HI, and NAb, respectively. The HI and NAb levels against the BPIV3 genotype A strain were 1:213 and 1:85 in calves, respectively. These results indicate that rHAd5-F + HN effectively induced immunity against BPIV3 infection.","PeriodicalId":23658,"journal":{"name":"Veterinary Research","volume":"55 1","pages":"144"},"PeriodicalIF":4.3,"publicationDate":"2024-11-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11545272/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142605115","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Recombinant characterization and pathogenicity of a novel L1C RFLP-1-4-4 variant of porcine reproductive and respiratory syndrome virus in China. 中国猪繁殖与呼吸综合征病毒新型 L1C RFLP-1-4-4 变异株的重组特征和致病性。

IF 3.7 1区农林科学

Veterinary Research Pub Date : 2024-11-06 DOI: 10.1186/s13567-024-01401-y

Xinyi Huang, Guoqing Liu, Tong Chang, Yongbo Yang, Tao Wang, Dasong Xia, Xinyu Qi, Xulong Zhu, Ziyi Wei, Xiaoxiao Tian, Haiwei Wang, Zhijun Tian, Xuehui Cai, Tongqing An

{"title":"Recombinant characterization and pathogenicity of a novel L1C RFLP-1-4-4 variant of porcine reproductive and respiratory syndrome virus in China.","authors":"Xinyi Huang, Guoqing Liu, Tong Chang, Yongbo Yang, Tao Wang, Dasong Xia, Xinyu Qi, Xulong Zhu, Ziyi Wei, Xiaoxiao Tian, Haiwei Wang, Zhijun Tian, Xuehui Cai, Tongqing An","doi":"10.1186/s13567-024-01401-y","DOIUrl":"10.1186/s13567-024-01401-y","url":null,"abstract":"Porcine reproductive and respiratory syndrome (PRRS) is one of the most significant diseases affecting the pig industry worldwide and is caused by the PRRS virus (PRRSV), which has complex genetic variation due to frequent mutations, indels, and recombination. The emergence of PRRSV L1C.5 in 2020 in the United States has raised worldwide concerns about PRRSV with the RFLP 1-4-4 pattern and lineage 1C. However, studies on the pathogenic characteristics, epidemiological distribution, and effectiveness of vaccines against PRRSV with L1C and RFLP1-4-4 pattern in China are still insufficient. In this study, a novel recombinant variant of PRRSV with RFLP 1-4-4 and lineage 1C features, different from L1C.5 in the United States, was isolated in China in 2021. In pathogenicity experiments in specific pathogen-free piglets or farm piglets, 60-100% of artificially infected experimental piglets died with high fever and respiratory symptoms. Inflammatory cytokine and chemokine levels were upregulated in infected piglets. A commercially modified live vaccine against highly pathogenic PRRSV did not provide effective protection when the vaccinated piglets were challenged with the novel L1C-1-4-4 variant. Therefore, this strain merits special attention when devising control and vaccine strategies. These findings suggest that extensive joint surveillance is urgently needed and that vaccine strategies should be updated to prevent the disease from spreading further.","PeriodicalId":23658,"journal":{"name":"Veterinary Research","volume":"55 1","pages":"142"},"PeriodicalIF":3.7,"publicationDate":"2024-11-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11539553/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142591790","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0