Veterinary Research最新文献

{"title":"Plasma metabolomics of Mycoplasma synoviae infection in SPF White Leghorn hens by liquid chromatography-tandem mass spectrometry.","authors":"Chun Wang, Qing Wang, Yang Li, Zhaoyang Wang, Bo Hou, Naiji Zhou, Weitao Cui, Sishun Hu, Yuncai Xiao, Wanpo Zhang, Hongbo Zhou, Zili Li, Zutao Zhou","doi":"10.1186/s13567-025-01494-z","DOIUrl":"10.1186/s13567-025-01494-z","url":null,"abstract":"<p><p>Mycoplasma synoviae (M. synoviae) is a major bacterial pathogen that causes serious economic losses in the global poultry industry. Systemic changes in specific pathogen free White Leghorn egg-laying hens after M. synoviae infection were investigated using intra-tracheally inoculated animals. Samples were collected 10 days post-infection (dpi) (204-day-old) and 52 dpi (246-day-old). Infection caused air sac lesion, footpad swelling and oviduct atrophy. The qPCR and in situ hybridization showed that bacteria colonized the trachea and oviduct, and that bacterial loads in the magnum and uterus were significantly higher than in the infundibulum and isthmus. Histopathological examination revealed increased tracheal mucosal thickening accompanied by inflammatory cell infiltration, and that tubular glands of the uterus were edematous or dissolved. Infection also induced decreased egg production and eggshell strength, and eggshell apex abnormalities appeared at 14 dpi. Plasma metabolomics of hens analyzed by liquid chromatography-tandem mass spectrometry showed 168 and 128 differentially-expressed metabolites (DEM) at 10 and 52 dpi, respectively. Pathway analysis revealed that DEM at 10 dpi were enriched in five distinctive pathways: regulation of the actin cytoskeleton, neuroactive ligand-receptor interaction, sphingolipid metabolism, gap junctions, and necroptosis. In contrast, DEM at 52 dpi were enriched in fifteen pathways involved in steroid hormone biosynthesis, ferroptosis, the calcium signaling pathway, apelin signaling pathway, progesterone-mediated oocyte maturation, and oocyte meiosis. Combined metabolic analysis demonstrated that changes in ethylsalicylate, nicotinamide, (3-Methoxy-4-hydroxyphenyl) ethylene glycol sulfate, sphingosine-1-phosphate (d18:1), carnitine C24:6, and 15(R)-prostaglandin E1 correlated the best with M. synoviae infection. This study provides new insights into understanding pathogen mechanisms and signposts novel treatments for M. synoviae infection in poultry.</p>","PeriodicalId":23658,"journal":{"name":"Veterinary Research","volume":"56 1","pages":"65"},"PeriodicalIF":3.7,"publicationDate":"2025-03-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11929215/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143693567","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Generation of an artificially attenuated fowl adenovirus 4 viral vector using the reverse genetics system based on full-length infectious clone.","authors":"Zhihui Tang, Dengfei Feng, Wentao Fan, Liping Yan, Suquan Song","doi":"10.1186/s13567-025-01496-x","DOIUrl":"10.1186/s13567-025-01496-x","url":null,"abstract":"<p><p>Fowl adenovirus serotype 4 (FAdV-4) is a non-enveloped double-stranded DNA virus with a 43-45 kb genome. This characteristic makes it a promising viral vector for expressing other antigens in developing multi-valent or multi-series vaccines in the poultry industry. To create an easy-to-use reverse genetics system for manipulating FAdV-4 genomic DNA, a full-length infectious clone of FAdV-4 was constructed using lambda Red-mediated recombination in Escherichia coli DH10B. Viable viruses were successfully rescued after the transfection of linearised infectious clones into LMH cells. The rescued viruses showed the same cytopathic effect and growth kinetics as wild-type FAdV-4 viruses. Based on the FAdV-4 infectious clone, the hexon coding sequence of the high-pathogenicity FAdV-4 was replaced by that of the nonpathogenic FAdV-4 using lambda Red-mediated recombination combined with rpsL counter selection without leaving extra sequences after engineering. The rescued recombinant virus was highly attenuated and showed low pathogenicity to 21-day-old SPF chickens. Hereto, the easy-to-use reverse genetics system for FAdV-4 was successfully established. With this platform, the genomic DNA of FAdV-4 can be manipulated and purified in DH10B, making it quicker and easier to generate a recombinant FAdV-4 virus to develop multi-valent/multi-series vaccines.</p>","PeriodicalId":23658,"journal":{"name":"Veterinary Research","volume":"56 1","pages":"62"},"PeriodicalIF":3.7,"publicationDate":"2025-03-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11929364/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143677334","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dynamic interplay between RNA N⁶-methyladenosine modification and porcine reproductive and respiratory syndrome virus infection.","authors":"Zi-Han Wang, Jing Li, Sai-Ya Ma, Meng-Xuan Liu, Yu-Fei Zhan, Feng Jin, Bing-Xin Liu, Wenjing Wang, Mei He, Yu-Chuan Yang, Yandong Tang, Peng Wang, Wuchao Zhang, Jie Tong","doi":"10.1186/s13567-025-01495-y","DOIUrl":"10.1186/s13567-025-01495-y","url":null,"abstract":"<p><p>N⁶-methyladenosine (m⁶A) has attracted significant attention for its role in regulating the complex interaction between viruses and host cells. Porcine reproductive and respiratory syndrome virus (PRRSV) is a significant pathogen affecting swine health worldwide. Here, we first identified seven m⁶A-enriched peaks in PRRSV genomic RNA by m⁶A RNA immunoprecipitation sequencing (m⁶A-seq). Moreover, functional analyses revealed a positive correlation between the m⁶A modification level and PRRSV replication. Treatment with the universal methylation inhibitor 3-deazaadenosine (3-DAA) effectively suppressed PRRSV replication in a dose-dependent manner. Furthermore, m⁶A-seq was also used to determine the m⁶A landscape of the transcriptome in PAMs infected with pandemic or highly pathogenic PRRSV strains. Among the 4677 transcripts exhibiting altered m⁶A modification levels, the MAPK14 gene and the p38/MAPK signalling pathway emerged as preliminary targets of m⁶A-mediated epigenetic regulation during PRRSV infection. These findings provide new insights into the epigenetic mechanisms underlying PRRSV infection and may facilitate the development of anti-PRRSV therapeutics.</p>","PeriodicalId":23658,"journal":{"name":"Veterinary Research","volume":"56 1","pages":"64"},"PeriodicalIF":3.7,"publicationDate":"2025-03-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11929310/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143693559","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular mechanisms of surface antigen suppression by ApiAP2 and its implications for vaccine development.","authors":"Peiyao Li, Rina Su, Ganglin Ren, Hongbin Si, Xingju Song, Dandan Hu","doi":"10.1186/s13567-025-01491-2","DOIUrl":"10.1186/s13567-025-01491-2","url":null,"abstract":"<p><p>The apicomplexan parasite Eimeria is the causative agent of coccidiosis, which results in enteritis in animals and imposes a significant economic burden on the farming industry. Apicomplexan Apetala2/ERF (ApiAP2) transcription factors are known to play crucial roles in various processes of Apicomplexa parasites. However, little is understood regarding the role of ApiAP2s in Eimeria species. In this study, we performed a functional characterisation of ApiAP2 through CRISPR-Cas9-mediated knockout. Our results revealed that the parasite's invasion efficiency, total oocyst output, and virulence were significantly impaired after EtAP2-S1 depletion. RNA sequencing (RNA-Seq) and Cleavage Under Targets and Tagmentation (CUT&Tag) analyses showed that EtAP2-S1 targets the promoters of numerous genes, and its knockout resulted in the up-regulation of 59 sag genes. Furthermore, the knockout strain exhibited significantly lower virulence while offering excellent immune protection, making it a promising candidate for a vaccine. This study demonstrates that EtAP2-S1 is a fitness-conferring gene that suppresses the expression of sag genes in E. tenella, which is the first step in developing gene-knockout vaccines to control coccidiosis.</p>","PeriodicalId":23658,"journal":{"name":"Veterinary Research","volume":"56 1","pages":"63"},"PeriodicalIF":3.7,"publicationDate":"2025-03-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11929185/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143693562","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correction: Glutathione reductase modulates endogenous oxidative stress and affects growth and virulence in Avibacterium paragallinarum.","authors":"Yan Zhi, Chen Mei, Zhenyi Liu, Ying Liu, Hongjun Wang","doi":"10.1186/s13567-025-01499-8","DOIUrl":"10.1186/s13567-025-01499-8","url":null,"abstract":"","PeriodicalId":23658,"journal":{"name":"Veterinary Research","volume":"56 1","pages":"61"},"PeriodicalIF":3.7,"publicationDate":"2025-03-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11927113/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143677329","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The inactivated and ISA 61 VG adjuvanted vaccine enhances protection against cross-serotype Listeria monocytogenes.","authors":"Fanzeng Meng, Ye Wang, Chao Chen, Tianxiang Pan, Jing Li, Yao Xu, Zegang Wang, Hao Yao, Xin'an Jiao, Yuelan Yin","doi":"10.1186/s13567-025-01483-2","DOIUrl":"10.1186/s13567-025-01483-2","url":null,"abstract":"<p><p>Listeriosis is a zoonotic disease caused by Listeria monocytogenes (Lm), posing a significant threat to the breeding industry and public health. Ruminant livestock are particularly susceptible to Lm,  thus effective strategies are needed for controlling ovine listeriosis. In this study, we developed two inactivated vaccines and evaluated their efficacy against Lm infection in murine and ovine models. We inactivated the Lm serotype 4h XYSN strain and adjuvanted it with water-in-oil ISA 61 VG (61 VG-AIV) or aluminum (Al-AIV). Pathological observations confirmed the safety of both vaccines in mice and sheep. The immunological assays demonstrated that, compared with the Al-AIV, the 61 VG-AIV induced higher levels of Lm-specific antibodies and proinflammatory cytokines, suggesting that the ISA 61 VG adjuvant has superior immunostimulatory effects compared with the alum adjuvant. 61 VG-AIV elicited greater immunoprotection than Al-AIV (83.4% vs. 50%) against serotype 4h Lm strain challenge in mice. Additionally, 61 VG-AIV afforded cross-protection against challenges with serotypes 1/2a, 1/2b, and 4b Lm strains. Importantly, high immunoprotection in sheep was conferred by the 61 VG-AIV group (83.4%). Taken together, our findings demonstrate that the ISA 61 VG adjuvant contributes to enhancing the humoral and cellular immune responses of inactivated Lm, and 61 VG-AIV is a promising vaccine candidate for the prevention and control of animal listeriosis. This research lays a solid foundation for its application in veterinary medicine.</p>","PeriodicalId":23658,"journal":{"name":"Veterinary Research","volume":"56 1","pages":"60"},"PeriodicalIF":3.7,"publicationDate":"2025-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11924870/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143671160","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Foot-and-mouth disease dynamics in multi-species livestock systems at the interface of African protected areas.","authors":"Oriane Ploquin, Vladimir Grosbois, Mthabisi Ndlovu, Simbarashe Ndozore, Martin Munzamba, Emildah Porovha, Khanyile Nkomo, Oriane Basso, Gaelle Corbel, Richard Shumba, Masocha D Mhlanga, Ellen Mwandirigana, Benjamin Musekiwa, Elijah Takayindisa, Anais Loisier, Hervé Fritz, Florian Liégeois, Alexandre Caron, Franck Prugnolle, Eve Miguel","doi":"10.1186/s13567-025-01487-y","DOIUrl":"10.1186/s13567-025-01487-y","url":null,"abstract":"<p><p>Many pathogens have the capacity to infect multiple hosts. Multi-species epidemiological systems are characterized by populations that interact and perform different functions in pathogen transmission and maintenance. This study investigated the epidemiological dynamics of foot-and-mouth disease (FMD) virus in cattle and goats and their respective functions in disease circulation within sympatric livestock populations adjacent to wildlife areas in Zimbabwe. Through year-long longitudinal serological monitoring, the spatial distributions of FMD antibodies and associated risk factors were examined. The results revealed significantly greater FMDV seroprevalence in cattle than in goats, with serostatus in cattle being influenced by proximity to wildlife areas. In contrast, goats presented a lower seroprevalence, less variation among age groups, and no association with proximity to protected areas. On the other hand, clustering analysis indicated the absence of clustering of seropositive individuals at the herd scale, suggesting low levels of virus transmission between animals belonging to the same herd in both species. These findings highlight the significance of context-dependent interactions among hosts, particularly with wildlife. This study emphasizes the necessity of comprehensive surveillance and strain identification across multiple sympatric species, both wild and domestic, for the effective management of multi-host pathogens. In conclusion, this research contributes to understanding the complex dynamics of FMD transmission in rural areas in Zimbabwe and emphasizes the importance of tailored surveillance strategies in diverse ecological settings.</p>","PeriodicalId":23658,"journal":{"name":"Veterinary Research","volume":"56 1","pages":"58"},"PeriodicalIF":3.7,"publicationDate":"2025-03-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11921631/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143664794","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Conserved function of bat IRF7 in activating antiviral innate immunity: insights into the innate immune response in bats.","authors":"Jie Wang, Qiuju Liu, Caixia Xu, Feiyu Fu, Qi Shao, Yapeng Fu, Zhaofei Wang, Jingjiao Ma, Hengan Wang, Yaxian Yan, Jianhe Sun, Yuqiang Cheng","doi":"10.1186/s13567-025-01490-3","DOIUrl":"10.1186/s13567-025-01490-3","url":null,"abstract":"<p><p>Bats are natural hosts for various highly pathogenic viruses, which pose a considerable threat to humans and animals. However, they rarely display signs of disease infection from these viruses. The expression of IRF7-induced IFN-β plays a crucial role in preventing viral infections. However, the role of bat IRF7 during viral infection remains unclear. In this study, we cloned Tadarida brasiliensis IRF7 and discovered that its amino acid sequence was poorly conserved among species. Next, we investigated the expression of bat IRF7 mRNA in Tadarida brasiliensis lung cells (TB 1 Lu) infected with RNA viruses such as Newcastle disease virus (NDV), avian influenza virus (AIV), vesicular stomatitis virus (VSV), and the double-stranded RNA (dsRNA) analogue poly (I:C) and demonstrated that these viral infections significantly upregulated the mRNA expression of bat IRF7. Furthermore, the overexpression of IRF7 in TB1 Lu cells activated the expression of bat innate immune-related genes and inhibited virus replication. Importantly, we observed that bat IRF7 function is highly conserved in avian and mammalian species. Structurally, we revealed that the IRF domain of bat IRF7 is essential for activating IFN-β. In summary, our findings indicate that bat IRF7 has a conserved ability to activate bat antiviral innate immunity. This study provides a theoretical foundation for further understanding the innate immune response in bats.</p>","PeriodicalId":23658,"journal":{"name":"Veterinary Research","volume":"56 1","pages":"59"},"PeriodicalIF":3.7,"publicationDate":"2025-03-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11921751/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143664793","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genetic analyses and functional validation of ruminant SLAMs reveal potential hosts for PPRV.","authors":"Xi Wei, Kejia Lu, Zhengwu Chang, Hanwei Guo, Qinfeng Li, Binxuan Yuan, Chen Liu, Zengqi Yang, Haijin Liu","doi":"10.1186/s13567-025-01489-w","DOIUrl":"10.1186/s13567-025-01489-w","url":null,"abstract":"<p><p>Peste des petits ruminants (PPR), caused by the peste des petits ruminants virus (PPRV), is a highly contagious disease affecting ruminants. While goats and sheep are well-known hosts, PPRV has also spread to wild ruminants, and it remains unclear which ruminant species can be infected. SLAM (Signaling lymphocytic activation molecule) acts as the primary receptor for PPRV, playing a crucial role in the viral infection process. Identifying which ruminant SLAMs can mediate PPRV infection is essential for understanding the potential hosts of PPRV, which is vital for effective eradication efforts. In this study, we first extracted 77 ruminant species' SLAM sequences from ruminant genome database. Based on these sequences, we predicted the structures of ruminant SLAMs. The analysis revealed that SLAM conformation is similar across ruminant species, and the potential PPRV H protein binding domain residues were conserved among SLAMs of these 77 species. Phylogenetic analysis of SLAM grouped ruminants into six families. We then selected representative SLAMs from each ruminant family to assess their role in PPRV infection. Our findings demonstrated that ruminant SLAMs efficiently mediated PPRV infection, with enhanced viral amplification observed in cells expressing SLAM from java mouse deer (Tragulidae) and goat (Bovidae), compared to cells expressing SLAM from white tailed deer (Cervidae) and giraffe (Giraffidae). These results underscore the need to consider a broader range of potential host populations beyond goat and sheep in efforts to prevent and eradicate PPRV.</p>","PeriodicalId":23658,"journal":{"name":"Veterinary Research","volume":"56 1","pages":"57"},"PeriodicalIF":3.7,"publicationDate":"2025-03-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11916873/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143658858","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"GHSR gene knockout alleviates the liver pathological response in Echinococcus granulosus infection by reducing parasite survival.","authors":"Jiang Zhu, Tanfang Zhou, Guangfeng Chen, Huijing Gao, Xia Chen, Ayinula Tuohetali, Ya Song, Dongming Pang, Kalibixiati Aimulajiang","doi":"10.1186/s13567-025-01478-z","DOIUrl":"10.1186/s13567-025-01478-z","url":null,"abstract":"<p><p>Cystic echinococcosis (CE) is a parasitic disease caused by the larval stage of Echinococcus granulosus, and the immunosuppressive microenvironment exacerbates disease progression. Ghrelin, a peptide hormone, plays a role in modulating immune inflammation and may influence the progression of E. granulosus infection through its receptor, GHSR (growth hormone secretagogue receptor). However, whether GHSR downregulation can inhibit E. granulosus infection remains unclear. In this study, we extracted liver tissues from E. granulosus-infected mice and those treated with the GHSR antagonist [D-Lys3]-GHRP-6. Proteomic analysis revealed 341 differentially expressed proteins, of which 185 were upregulated and 156 were downregulated. Metabolomic sequencing revealed 101 differentially expressed metabolites, including 62 upregulated and 39 downregulated metabolites. KEGG pathway enrichment analysis of both proteomic and metabolomic data revealed seven key signalling pathways, 11 key proteins, and 26 key metabolites that interact through metabolic and organic system networks. Next, we examined the disease progression of E. granulosus infection in GHSR-knockout mice. Compared with the E. granulosus (Eg) group, the GHSR-KO group presented a significant reduction in the number of liver infection foci. The serum and liver ghrelin levels were significantly greater in the E. granulosus group than in the control group, along with increased secretion of proinflammatory cytokines (IL-2 and IFN-γ) and decreased secretion of anti-inflammatory cytokines (IL-4 and IL-10). In contrast, the GHSR-KO group presented significantly lower ghrelin levels in both the serum and liver, with reduced proinflammatory cytokine secretion and increased anti-inflammatory cytokine secretion, similar to those of the control group. Furthermore, ghrelin and inflammation-related factors, including MyD88, NF-κB p65, iNOS, and Arg-1, exhibited coordinated expression changes in liver lesions and surrounding areas. These findings suggest that GHSR gene knockout can ameliorate the progression of liver E. granulosus infection and associated liver inflammation.</p>","PeriodicalId":23658,"journal":{"name":"Veterinary Research","volume":"56 1","pages":"55"},"PeriodicalIF":3.7,"publicationDate":"2025-03-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11895129/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143597818","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}