Veterinary Research最新文献

{"title":"Development of a reporter feline herpesvirus-1 for antiviral screening assays.","authors":"Jia Yang, Li Li, Fuqiang Xu, Fan Jia","doi":"10.1186/s13567-024-01430-7","DOIUrl":"https://doi.org/10.1186/s13567-024-01430-7","url":null,"abstract":"<p><p>Feline herpesvirus type 1 (FHV-1), a member of the Herpesviridae family, is one of the most important pathogens that causes upper respiratory tract disease in felines. Following infection, FHV-1 can spread retrogradely to the trigeminal ganglia, establishing a life-long latency. Although vaccines are available for routine feline vaccination, FHV-1 is still an agent that poses a serious threat to feline health. There are currently no specific drugs for the treatment of FHV-1. To facilitate the screening of antiviral drugs, we constructed a reporter FHV-1 virus, which expresses a secreted Gaussia luciferase (GLuc) and a bright green fluorescent protein, mNeonGreen. The reporter virus shows slower growth than does the wild-type FHV-1. The expression of the two reporter genes, Gluc and mNeonGreen, was consistent with viral propagation and remained stable during continuous passage in CRFK cells, even after twenty rounds. In addition, the known inhibitor ganciclovir was used to confirm the characteristics of the reporter virus for drug screening. We found that the reporter FHV-1 is suitable for antiviral screening assays. Overall, our work provides a useful tool for screening drugs to combat FHV-1.</p>","PeriodicalId":23658,"journal":{"name":"Veterinary Research","volume":"55 1","pages":"167"},"PeriodicalIF":3.7,"publicationDate":"2024-12-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142855589","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The predominant role of FliC contributes to the flagella-related pathogenicity of ST34 S. Typhimurium monophasic variant.","authors":"Yaming Hong, Qilong Hou, Hui Liu, Xiaojie Wang, Jiaojie Gu, Zhenyu Wang, Xinan Jiao, Qiuchun Li","doi":"10.1186/s13567-024-01427-2","DOIUrl":"https://doi.org/10.1186/s13567-024-01427-2","url":null,"abstract":"<p><p>Over the past two decades, the monophasic variant of Salmonella enterica serovar Typhimurium (S. Typhimurium) has rapidly emerged and increased worldwide. This upsurge is especially true for the European clone of the ST34 S. Typhimurium monophasic variant. The key distinction between ST34 S. Typhimurium and its monophasic variant is that the genes that encode for second-phase flagellin (FljB) and the regions around it have been replaced with various multidrug resistance cassettes. To determine if the loss of fljB or the retention of fliC,-the gene coding for first-phase flagellin (FliC)-, would impact its pathogenicity, we constructed various mutations, including deletions of fljB, fliC, fliC/fljB, and strains where fliC was replaced with fljB. Our results showed that the loss of fljB in ST34 S. Typhimurium and its monophasic variant does not affect bacterial motility, cell infection ability, survival in macrophages, induced pro-inflammatory cytokines secretion, virulence, or persistent infection in mice. However, the deletion of fliC caused a significant decrease in these outcomes for both strains, while the replacement of fliC with fljB only partially restored these capabilities. Consequently, we determined that FliC is predominant in the flagellar expression of ST34 S. Typhimurium other than FljB. This finding demonstrates that replacing the fljB gene with various resistance regions in ST34 S. Typhimurium monophasic variants can enhance bacterial survival under specific antibiotic farming practices and spread globally.</p>","PeriodicalId":23658,"journal":{"name":"Veterinary Research","volume":"55 1","pages":"166"},"PeriodicalIF":3.7,"publicationDate":"2024-12-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142855650","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Serotype diversity of Actinobacillus pleuropneumoniae detected by real-time PCR in clinical and subclinical samples from Spanish pig farms during 2017-2022.","authors":"José Luis Arnal Bernal, Marcelo Gottschalk, Sonia Lacotoure, Celia Sanz Tejero, Gema Chacón Pérez, Desiree Martín-Jurado, Ana Belén Fernández Ros","doi":"10.1186/s13567-024-01419-2","DOIUrl":"https://doi.org/10.1186/s13567-024-01419-2","url":null,"abstract":"<p><p>Actinobacillus pleuropneumoniae is the causative agent of porcine pleuropneumonia, a challenging respiratory disease for the global swine industry. Variations in the serotypes associated with clinical disease have been observed in different regions worldwide. This study aimed to provide an updated epidemiological assessment of A. pleuropneumoniae serotypes in Spain, incorporating bacterial characterization through serotyping and toxinotyping. Serotypes 9/11, 2, 4, 5, 17, and 13 were frequently identified in diseased animals. Furthermore, qPCR of lung samples from an outbreak, even when samples were pooled, emerged as a robust diagnostic tool, enabling the rapid detection of A. pleuropneumoniae and their serotypes without the need for microbiological isolation. This technology also facilitates serotype monitoring of apparently healthy herds through the testing of oral fluids. The study revealed the frequent simultaneous presence of diverse serotypes within a farm. Serotypes 1, 7, 10, 12, 18, and 19 were frequently found in subclinically infected animals but were rarely detected in acute pleuropneumonia outbreaks in the current study. These results provide valuable information for interpreting the potential virulence of the different serotypes in Spain. However, other predisposing factors and the immune status of the herds such as type of vaccines used when appropriate, should be carefully considered before drawing definitive conclusions. Nevertheless, the study offers valuable insights that underscore the necessity for detailed regional data to contribute toward a comprehensive understanding of the disease dynamics and toward formulating effective control measures for porcine pleuropneumonia.</p>","PeriodicalId":23658,"journal":{"name":"Veterinary Research","volume":"55 1","pages":"165"},"PeriodicalIF":3.7,"publicationDate":"2024-12-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142855646","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"When animal viruses meet N⁶-methyladenosine (m⁶A) modifications: for better or worse?","authors":"Wenjing Wang, Yufei Jin, Ziyun Xie, Mei He, Jing Li, Zihan Wang, Saiya Ma, Wuchao Zhang, Jie Tong","doi":"10.1186/s13567-024-01424-5","DOIUrl":"https://doi.org/10.1186/s13567-024-01424-5","url":null,"abstract":"<p><p>N⁶-methyladenosine (m⁶A) is a prevalent and dynamic RNA modification, critical in regulating gene expression. Recent research has shed light on its significance in the life cycle of viruses, especially animal viruses. Depending on the context, these modifications can either enhance or inhibit the replication of viruses. However, research on m⁶A modifications in animal virus genomes and the impact of viral infection on the host cell m⁶A landscape has been hindered due to the difficulty of detecting m⁶A sites at a single-nucleotide level. This article summarises the methods for detecting m⁶A in RNA. It then discusses the progress of research into m⁶A modification within animal viruses' infections, such as influenza A virus, porcine epidemic diarrhoea virus, porcine reproductive, and respiratory syndrome virus. Finally, the review explores how m⁶A modification affects the following three aspects of the replication of animal RNA viruses: the regulation of viral genomic RNA function, the alteration of the m⁶A landscape in cells after viral infection, and the modulation of antiviral immunity through m⁶A modification. Research on m⁶A modifications in viral RNA sheds light on virus-host interactions at a molecular level. Understanding the impact of m⁶A on viral replication can help identify new targets for antiviral drug development and may uncover novel regulatory pathways that could potentially enhance antiviral immune responses.</p>","PeriodicalId":23658,"journal":{"name":"Veterinary Research","volume":"55 1","pages":"171"},"PeriodicalIF":3.7,"publicationDate":"2024-12-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142855652","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biofilm formation in Streptococcus suis: in vitro impact of serovars and assessment of coinfections with other porcine respiratory disease complex bacterial pathogens.","authors":"Rubén Miguélez-Pérez, Oscar Mencía-Ares, César B Gutiérrez-Martín, Alba González-Fernández, Máximo Petrocchi-Rilo, Mario Delgado-García, Sonia Martínez-Martínez","doi":"10.1186/s13567-024-01412-9","DOIUrl":"https://doi.org/10.1186/s13567-024-01412-9","url":null,"abstract":"<p><p>Streptococcus suis is a worldwide pathogen that impacts the swine industry, causing severe clinical signs, including meningitis and arthritis, in postweaning piglets. A key virulence mechanism of S. suis is biofilm formation, which improves its persistence and resistance to external factors. Here, we assessed the in vitro biofilm formation of 240 S. suis isolates from Spanish swine farms and evaluated the effects of serovars (SVs) and coinfections with other porcine respiratory disease complex (PRDC) pathogens. Our study revealed significant heterogeneity in biofilm formation among S. suis SVs. Notably, SV2 resulted in the lowest degree of biofilm formation, in contrast with the high biofilm-forming capacities of SV1, SV7, and SV9. Other PRDC pathogens, including Actinobacillus pleuropneumoniae, Glaesserella parasuis, and Pasteurella multocida, formed biofilms, although they were generally less robust than those of S. suis (except for SV2), which contrasts with the high biofilm formation of Staphylococcus hyicus. Coinfections enhanced biofilm formation in mixed cultures of S. suis, particularly with P. multocida. Other coinfections revealed variable results in pathogen interactions, suggesting the potential of biofilms for increased persistence and pathogenicity in coinfections. In conclusion, this study underscores the importance of serovar-specific differences in biofilm formation among S. suis isolates, with significant implications for pathogenicity and persistence. The heterogeneous biofilm formation observed in coinfections with other PRDC pathogens reveals a complex interplay that could exacerbate disease severity. These findings provide a foundation for further research on biofilm mechanisms to mitigate the impact of PRDC in the swine industry.</p>","PeriodicalId":23658,"journal":{"name":"Veterinary Research","volume":"55 1","pages":"157"},"PeriodicalIF":3.7,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11610180/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142772727","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Porcine parvovirus infection induces necroptosis of porcine placental trophoblast cells via a ZBP1-mediated pathway.","authors":"Ning Xu, Qian Du, Yijiao Cheng, Lichen Nie, Peipei Ma, Dingwen Feng, Yong Huang, Dewen Tong","doi":"10.1186/s13567-024-01410-x","DOIUrl":"10.1186/s13567-024-01410-x","url":null,"abstract":"<p><p>Porcine parvovirus (PPV) infection induces germ cell death, leading to reproductive disorders in first-pregnant sows. Porcine placental trophoblast cells (PTCs) are the major target of PPV, and we have previously found that PPV infection leads to the death of PTCs by a non-apoptotic process, which may be related to PPV pathogenicity. The Z-nucleic acid-binding protein 1 (ZBP1) is often activated after virus invasion and mediates subsequent cell death. Here, we found that PPV infection induced ZBP1-mediated necroptosis of porcine PTCs in the presence of the apoptosis inhibitor, AC-DEVD-CHO. ZBP1 expression was upregulated during PPV infection, and ZBP1 knockout or RNA interference significantly reduced its expression along with the PPV-induced necroptosis. We discovered that RIPK3 and MLKL, but not Caspase-8, were involved in downstream signaling of ZBP1 during PPV infection; the phosphorylation levels of RIPK3 and MLKL were enhanced, but Caspase-8 was not significantly cleaved. The knockout of RIPK3 and MLKL significantly reduced the PPV infection-induced necroptosis of porcine PTCs. RIPK3 knockout did not affect the PPV infection-induced upregulation of ZBP1 expression, but blocked the activation of MLKL. MLKL knockout did not affect the upregulation of ZBP1 expression and RIPK3 phosphorylation during PPV infection. UV-inactivated PPV induced significantly less necroptosis of porcine PTCs than non-irradiated PPV. A PPV strain with a mutation in the translation initiation codon was still able to induce necroptosis of PTCs through the ZBP1/RIPK3/MLKL pathway. These results provide new insights into the pathogenic mechanism of PPV infection and suggest that PPV infection of porcine PTCs induces necroptosis through the viral DNA-dependent ZBP1/RIPK3/MLKL pathway.</p>","PeriodicalId":23658,"journal":{"name":"Veterinary Research","volume":"55 1","pages":"156"},"PeriodicalIF":3.7,"publicationDate":"2024-11-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11605877/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142755717","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Insights from the 2023 International Veterinary Immunology Symposium: global perspectives at Kruger National Park.","authors":"Alejandra V Capozzo, Yolanda Corripio-Miyar, Yolandy Lemmer","doi":"10.1186/s13567-024-01409-4","DOIUrl":"10.1186/s13567-024-01409-4","url":null,"abstract":"<p><p>The 13th International Veterinary Immunology Symposium (IVIS) was initially due to take place in August 2022, but as many things in our lives, the COVID-19 pandemic that hit the world two years prior, forced the organising committee to postpone the meeting until November 2023. As it is tradition, the veterinary immunology symposium was organised as a satellite meeting of the IUIS International Congress of Immunology, which in 2023 took place in Cape Town, and it is where veterinary immunologists from all over the world get together to discuss advances and challenges in the field of animal health. The 2023 International Veterinary Immunology Symposium (IVIS) was held from November 17th to 21st at Kruger National Park, Skukuza, South Africa. This was the first time the symposium was hosted on the African continent. This event gathered 210 veterinary professionals and scientists from 38 countries to discuss the latest advancements and challenges in veterinary immunology. A highlight of the event was that over 70% of the delegates were first-time attendees, contributing to the symposium's global reach. The symposium featured a series of 83 oral presentations and 104 poster presentations, including topics relating to protective immunity, vaccine strategies, important disease targets, and methodological advancements in veterinary immunology. Workshops provided hands-on experiences and discussions on new technologies such as next-generation sequencing and vaccine development strategies against bacterial infections. The symposium also provided opportunities for networking and engagements with leaders in the field, set against the backdrop of one of Africa's most iconic game reserves, enhancing the experience with a unique blend of professional exchange and natural beauty.</p>","PeriodicalId":23658,"journal":{"name":"Veterinary Research","volume":"55 1","pages":"155"},"PeriodicalIF":3.7,"publicationDate":"2024-11-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11590537/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142733090","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Avian influenza virus circulation and immunity in a wild urban duck population prior to and during a highly pathogenic H5N1 outbreak.","authors":"Jordan Wight, Ishraq Rahman, Hannah L Wallace, Joshua T Cunningham, Sheena Roul, Gregory J Robertson, Rodney S Russell, Wanhong Xu, Dmytro Zhmendak, Tamiru N Alkie, Yohannes Berhane, Kathryn E Hargan, Andrew S Lang","doi":"10.1186/s13567-024-01397-5","DOIUrl":"10.1186/s13567-024-01397-5","url":null,"abstract":"<p><p>Highly pathogenic avian influenza (HPAI) H5N1 clade 2.3.4.4b viruses were first detected in St. John's, Canada in late 2021. To investigate the patterns of avian influenza virus (AIV) infection and immune responses subsequent to the arrival of H5N1, we sampled the wild urban duck population in this area for a period of 16 months after the start of the outbreak and compared these findings to those from archived samples. Antibody seroprevalence was relatively stable before the outbreak (2011-2014) at 27.6% and 3.9% for anti-AIV (i.e., NP) and H5-specific antibodies, respectively. During the winter of 2022, AIV-NP and H5-specific antibody seroprevalence both reached 100%, signifying a population-wide infection event, which was observed again in late February 2023 following a second H5N1 incursion from Eurasia. As expected, population-level immunity waned over time, with ducks seropositive for anti-AIV-NP antibodies for approximately twice as long as for H5-specific antibodies, with the population seronegative to the latter after approximately six months. We observed a clear relationship of increasing antibody levels with decreasing viral RNA loads that allowed for interpretation of the course of infection and immune response in infected individuals and applied these findings to two cases of resampled ducks to infer infection history. Our study highlights the value of applying both AIV surveillance and seroprevalence monitoring to provide a better understanding of AIV dynamics in wild populations, which may be crucial following the global dissemination of clade 2.3.4.4b H5Nx subtypes to assess the threats they pose to both wild and domestic animals, and to humans.</p>","PeriodicalId":23658,"journal":{"name":"Veterinary Research","volume":"55 1","pages":"154"},"PeriodicalIF":3.7,"publicationDate":"2024-11-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11585116/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142692938","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A pH-triggered self-releasing humic acid hydrogel loaded with porcine interferon α/γ achieves anti-pseudorabies virus effects by oral administration.","authors":"Maoyuan Sun, Yongli Shi, Baishi Lei, Wuchao Zhang, Jingjing Feng, Shenghu Ge, Wanzhe Yuan, Kuan Zhao","doi":"10.1186/s13567-024-01411-w","DOIUrl":"10.1186/s13567-024-01411-w","url":null,"abstract":"<p><p>Interferon α (IFNα) and interferon γ (IFNγ) play pivotal roles in mediating crucial biological functions, including antiviral activity and immune regulation. However, the efficacy of monomeric IFN is limited, and its administration relies solely on injection. To address this issue, we successfully expressed and purified a recombinant porcine IFNα and IFNγ fusion protein (rPoIFNα/γ). Furthermore, we developed a pH-triggered humic acid hydrogel delivery system that effectively protects rPoIFNα/γ from gastric acid degradation, enhancing its oral bioavailability. Neither the humic acid hydrogel nor rPoIFNα/γ exhibited cytotoxic effects on porcine kidney-15 (PK-15) cells in vitro. The replication of vesicular stomatitis virus and pseudorabies virus (PRV) was effectively inhibited by rPoIFNα/γ, resulting in an antiviral activity of approximately 10⁴ U/mL. Scanning electron microscopy revealed that the humic acid hydrogel had a loose and porous honeycomb structure. The IFNα/γ@PAM^gel hydrogel effectively adsorbed rPoIFNα/γ, as confirmed by Fourier transform infrared spectroscopy analysis, demonstrating a favourable IFN-loading capacity. In vitro experiments revealed that IFNα/γ@PAM^gel swelled and released IFNα/γ rapidly at pH 7.4 but not at pH 1.2. The oral administration of IFNα/γ@PAM^gel in mice enhanced the proliferation and differentiation of CD4⁺ and CD8⁺ cells. Additionally, mice infected with PRV and treated with IFNα/γ@PAM^gel presented increased transcription levels of interferon-stimulated genes in the serum, reduced mortality rates, lower viral loads in various tissues, and decreased levels of organ damage. In conclusion, this study demonstrates that orally administered IFNα/γ@PAM^gel has antiviral and immunomodulatory effects, highlighting its potential as a therapeutic agent against PRV infection.</p>","PeriodicalId":23658,"journal":{"name":"Veterinary Research","volume":"55 1","pages":"153"},"PeriodicalIF":3.7,"publicationDate":"2024-11-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11580204/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142682885","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correction: Blastocystis occurrence and subtype diversity in European wild boar (Sus scrofa) from the Iberian Peninsula.","authors":"Pamela C Köster, Ana M Figueiredo, Jenny G Maloney, Alejandro Dashti, Begoña Bailo, Rita T Torres, Carlos Fonseca, Atle Mysterud, Miguel Á Habela, Antonio Rivero-Juarez, Joaquín Vicente, Emmanuel Serrano, Maria C Arnal, Daniel Fernández de Luco, José A Armenteros, Ana Balseiro, Guillermo A Cardona, João Carvalho, Dário Hipólito, Joana Fernandes, Josman D Palmeira, Rafael Calero-Bernal, David González-Barrio, Monica Santin, David Carmena","doi":"10.1186/s13567-024-01408-5","DOIUrl":"10.1186/s13567-024-01408-5","url":null,"abstract":"","PeriodicalId":23658,"journal":{"name":"Veterinary Research","volume":"55 1","pages":"152"},"PeriodicalIF":3.7,"publicationDate":"2024-11-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11566639/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142629475","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}