Veterinary Research最新文献_第2页

A chimeric strain of porcine reproductive and respiratory syndrome virus 2 derived from HP-PRRSV and NADC30-like PRRSV confers cross-protection against both strains. 从 HP-PRRSV 和 NADC30-like PRRSV 提取的猪繁殖与呼吸综合征病毒 2 嵌合株对这两种病毒株具有交叉保护作用。

IF 3.7 1区农林科学

Veterinary Research Pub Date : 2024-10-07 DOI: 10.1186/s13567-024-01390-y

Yang Li, Yumiao Wang, Xiuxiu Pei, Shao Chen, Yang Jing, Yongshuai Wu, Zhiqian Ma, Zhiwei Li, Zifang Zheng, Yingtong Feng, Lele Xu, Xiao Liu, Xuyang Guo, Haixue Zheng, Shuqi Xiao

{"title":"A chimeric strain of porcine reproductive and respiratory syndrome virus 2 derived from HP-PRRSV and NADC30-like PRRSV confers cross-protection against both strains.","authors":"Yang Li, Yumiao Wang, Xiuxiu Pei, Shao Chen, Yang Jing, Yongshuai Wu, Zhiqian Ma, Zhiwei Li, Zifang Zheng, Yingtong Feng, Lele Xu, Xiao Liu, Xuyang Guo, Haixue Zheng, Shuqi Xiao","doi":"10.1186/s13567-024-01390-y","DOIUrl":"https://doi.org/10.1186/s13567-024-01390-y","url":null,"abstract":"Porcine reproductive and respiratory syndrome (PRRS) is one of the most significant swine viral infectious diseases worldwide. Vaccination is a key strategy for the control and prevention of PRRS. At present, the NADC30-like PRRSV strain has become the predominant epidemic strain in China, superseding the HP-PRRSV strain. The existing commercial vaccines offer substantial protection against HP-PRRSV, but their efficacy against NADC30-like PRRSV is limited. The development of a novel vaccine that can provide valuable cross-protection against both NADC30-like PRRSV and HP-PRRSV is highly important. In this study, an infectious clone of a commercial MLV vaccine strain, GD (HP-PRRSV), was first generated (named rGD). A recombinant chimeric PRRSV strain, rGD-SX-5U2, was subsequently constructed by using rGD as a backbone and embedding several dominant immune genes, including the NSP2, ORF5, ORF6, and ORF7 genes, from an NADC30-like PRRSV isolate. In vitro experiments demonstrated that chimeric PRRSV rGD-SX-5U2 exhibited high tropism for MARC-145 cells, which is of paramount importance in the production of PRRSV vaccines. Moreover, subsequent in vivo inoculation and challenge experiments demonstrated that rGD-SX-5U2 confers cross-protection against both HP-PRRSV and NADC30-like PRRSV, including an improvement in ADG levels and a reduction in viremia and lung tissue lesions. In conclusion, our research demonstrated that the chimeric PRRSV strain rGD-SX-5U2 is a novel approach that can provide broad-spectrum protection against both HP-PRRSV and NADC30-like PRRSV. This may be a significant improvement over previous MLV vaccinations.","PeriodicalId":23658,"journal":{"name":"Veterinary Research","volume":null,"pages":null},"PeriodicalIF":3.7,"publicationDate":"2024-10-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11460240/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142393746","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Strategic nucleic acid detection approaches for diagnosing African swine fever (ASF): navigating disease dynamics. 诊断非洲猪瘟（ASF）的战略性核酸检测方法：疾病动态导航。

IF 3.7 1区农林科学

Veterinary Research Pub Date : 2024-10-07 DOI: 10.1186/s13567-024-01386-8

Yuanshou Zhu, Meng Zhang, Zhijun Jie, Shujuan Guo, Zhigang Zhu, Sheng-Ce Tao

{"title":"Strategic nucleic acid detection approaches for diagnosing African swine fever (ASF): navigating disease dynamics.","authors":"Yuanshou Zhu, Meng Zhang, Zhijun Jie, Shujuan Guo, Zhigang Zhu, Sheng-Ce Tao","doi":"10.1186/s13567-024-01386-8","DOIUrl":"https://doi.org/10.1186/s13567-024-01386-8","url":null,"abstract":"African swine fever (ASF) is a devastating disease caused by African swine fever virus (ASFV) and leads to significant economic losses in the pig farming industry. Given the absence of an effective vaccine or treatment, the mortality rate of ASF is alarmingly close to 100%. Consequently, the ability to rapidly and accurately detect ASFV on site and promptly identify infected pigs is critical for controlling the spread of this pandemic. The dynamics of the ASF virus load and antibody response necessitate the adoption of various detection strategies at different stages of infection, a topic that has received limited attention to date. This review offers detailed guidance for choosing appropriate ASF diagnostic techniques tailored to the clinical manifestations observed from the acute to chronic phases, including asymptomatic cases. We comprehensively summarize and evaluate the latest advancements in ASFV detection methods, such as CRISPR-based diagnostics, biosensors, and microfluidics. Additionally, we address the challenges of false negatives or positives due to ASF variants or the use of injected live attenuated vaccines. This review provides an exhaustive list of diagnostic tests suitable for detecting each stage of symptoms and potential target genes for developing new detection methods. In conclusion, we highlight the current challenges and future directions in ASFV detection, underscoring the need for continued research and innovation in this field.","PeriodicalId":23658,"journal":{"name":"Veterinary Research","volume":null,"pages":null},"PeriodicalIF":3.7,"publicationDate":"2024-10-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11460097/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142393749","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Transcriptome analysis reveals a new virulence-associated trimeric autotransporter responsible for Glaesserella parasuis autoagglutination. 转录组分析揭示了一种新的与毒力相关的三聚体自体转运体，该转运体负责寄生褐喉蛆虫的自体凝集。

IF 3.7 1区农林科学

Veterinary Research Pub Date : 2024-10-07 DOI: 10.1186/s13567-024-01387-7

Junxing Li, Shiyi Ye, Fei Su, Bin Yu, Lihua Xu, Hongchao Sun, Xiufang Yuan

{"title":"Transcriptome analysis reveals a new virulence-associated trimeric autotransporter responsible for Glaesserella parasuis autoagglutination.","authors":"Junxing Li, Shiyi Ye, Fei Su, Bin Yu, Lihua Xu, Hongchao Sun, Xiufang Yuan","doi":"10.1186/s13567-024-01387-7","DOIUrl":"https://doi.org/10.1186/s13567-024-01387-7","url":null,"abstract":"Capsular polysaccharide is an important virulence factor of Glaesserella parasuis. An acapsular mutant displays multiple phenotype variations, while the underlying mechanism for these variations is unknown. In this study, we created an acapsular mutant by deleting the wza gene in the capsule locus. We then used transcriptome analysis to compare the gene expression profiles of the wza deletion mutant with those of the parental strain to understand the possible reasons for the phenotypic differences. The mutant Δwza, which has a deleted wza gene, secreted less polysaccharide and lost its capsule structure. The Δwza exhibited increased autoagglutination, biofilm formation and adherence to eukaryotic cells, while the complementary strain C-Δwza partially restored the phenotype. Transcriptome analysis revealed several differentially expressed genes (DEGs) in Δwza, including up-regulated outer membrane proteins and proteins involved in peptidoglycan biosynthesis, suggesting that wza deletion affects the cell wall homeostasis of G. parasuis. Transcriptome analysis revealed the contribution of non-coding RNAs in the regulation of DEGs. Moreover, a new virulence-associated trimeric autotransporter, VtaA31 is upregulated in Δwza. It is responsible for enhanced autoagglutination but not for enhanced biofilm formation and adherence to eukaryotic cells in Δwza. In conclusion, these data indicate that wza affects the expression of multiple genes, especially those related to cell wall synthesis. Furthermore, they provide evidence that vtaA31 is involved in the autoagglutination of G. parasuis.","PeriodicalId":23658,"journal":{"name":"Veterinary Research","volume":null,"pages":null},"PeriodicalIF":3.7,"publicationDate":"2024-10-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11460128/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142393750","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Blastocystis occurrence and subtype diversity in European wild boar (Sus scrofa) from the Iberian Peninsula. 伊比利亚半岛欧洲野猪（Sus scrofa）中的布氏囊虫发生率和亚型多样性。

IF 3.7 1区农林科学

Veterinary Research Pub Date : 2024-10-07 DOI: 10.1186/s13567-024-01385-9

Pamela C Köster, Ana M Figueiredo, Jenny G Maloney, Alejandro Dashti, Begoña Bailo, Rita T Torres, Carlos Fonseca, Atle Mysterud, Miguel Á Habela, Antonio Rivero-Juarez, Joaquín Vicente, Emmanuel Serrano, Maria C Arnal, Daniel Fernández de Luco, José A Armenteros, Ana Balseiro, Guillermo A Cardona, João Carvalho, Dário Hipólito, Joana Fernandes, Josman D Palmeira, Rafael Calero-Bernal, David González-Barrio, Monica Santin, David Carmena

{"title":"Blastocystis occurrence and subtype diversity in European wild boar (Sus scrofa) from the Iberian Peninsula.","authors":"Pamela C Köster, Ana M Figueiredo, Jenny G Maloney, Alejandro Dashti, Begoña Bailo, Rita T Torres, Carlos Fonseca, Atle Mysterud, Miguel Á Habela, Antonio Rivero-Juarez, Joaquín Vicente, Emmanuel Serrano, Maria C Arnal, Daniel Fernández de Luco, José A Armenteros, Ana Balseiro, Guillermo A Cardona, João Carvalho, Dário Hipólito, Joana Fernandes, Josman D Palmeira, Rafael Calero-Bernal, David González-Barrio, Monica Santin, David Carmena","doi":"10.1186/s13567-024-01385-9","DOIUrl":"https://doi.org/10.1186/s13567-024-01385-9","url":null,"abstract":"The ongoing increase in wild boar populations across Europe has fostered human-wildlife conflicts, including the transmission of emerging pathogens with zoonotic importance. Blastocystis is a ubiquitous, faecal-oral transmitted protist that can cause gastrointestinal illnesses and is observed in humans and animals worldwide. The role of wildlife in the epidemiology of Blastocystis is insufficiently understood. Thus, we investigated the occurrence and subtype diversity of Blastocystis in free-ranging wild boars from the Iberian Peninsula using conventional PCR and next-generation amplicon sequencing of a fragment of the ssu RNA gene. A total of 459 wild boar faecal samples were collected across Spain (n = 360) and Portugal (n = 99) between 2014 and 2021. Blastocystis was present in 15.3% (70/459; 95% CI 12.1-18.9) of the wild boars analysed, and its occurrence was significantly higher in Portugal (34.3%, 34/99; 95% CI 25.1-44.6) than in Spain (10.0%, 36/360; 95% CI 7.1-13.6). Seven Blastocystis subtypes (ST5, ST10b, ST13-ST15, ST24b, and ST43) were detected among the surveyed wild boar populations, with greater variability detected in Portuguese samples. ST5 was identified in all the Blastocystis-positive animals, whereas 14.3% of them harboured ST mixed colonisations. Our results demonstrate that Blastocystis ST5 is particularly adapted to infect wild boars. The additional identification of zoonotic STs reinforces the role of wild boars as spreaders of zoonotic infections with public health significance.","PeriodicalId":23658,"journal":{"name":"Veterinary Research","volume":null,"pages":null},"PeriodicalIF":3.7,"publicationDate":"2024-10-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11460206/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142393747","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Spatio-temporal distribution and international context of bovine viral diarrhoea virus genetic diversity in France. 法国牛病毒性腹泻病毒基因多样性的时空分布和国际背景。

IF 3.7 1区农林科学

Veterinary Research Pub Date : 2024-10-03 DOI: 10.1186/s13567-024-01377-9

Claire Lescoat, Delphine Perrotte, Séverine Barry, Élise Oden, Valentin Herbet, Gaël Beaunée, Marc Tabouret, Fabienne Benoit, Pierre-Hugues Pitel, Véronique Duquesne, Xavier Bailly, Julien Thézé, Guy Kouokam

{"title":"Spatio-temporal distribution and international context of bovine viral diarrhoea virus genetic diversity in France.","authors":"Claire Lescoat, Delphine Perrotte, Séverine Barry, Élise Oden, Valentin Herbet, Gaël Beaunée, Marc Tabouret, Fabienne Benoit, Pierre-Hugues Pitel, Véronique Duquesne, Xavier Bailly, Julien Thézé, Guy Kouokam","doi":"10.1186/s13567-024-01377-9","DOIUrl":"10.1186/s13567-024-01377-9","url":null,"abstract":"Bovine viral diarrhoea (BVD) is one of the most economically damaging livestock enzootic diseases in the world. BVD aetiological agents are three pestiviruses (BVDV-1, -2 and HoBi-like pestivirus), which exhibit high genetic diversity and complex transmission cycles. This considerably hampers the management of the disease, which is why eradication plans have been implemented in several countries. In France, a national plan has been in place since 2019. Our understanding of its impact on the distribution of BVDV genotypes is limited by the availability of French genetic data. Here, we conducted a molecular epidemiology study to refine our knowledge of BVDV genetic diversity in France, characterise its international relationships, and analyse national spatio-temporal genotypic distribution. We collated 1037 BVDV-positive samples throughout France between 2011 and 2023, with a greater sampling effort in two major cattle production areas. We developed a high-throughput sequencing protocol which we used to complete the 5'UTR genotyping of this collection. We show that two main BVDV-1 genotypes, 1e and 1b, account for 88% of genotyped sequences. We also identified seven other BVDV-1 genotypes occurring at low frequencies and three BVDV-2 samples (genotype 2c). Phylogenetic analyses indicate different worldwide distribution patterns between the two main BVDV-1 genotypes. Their relative frequencies present no major changes in France since the 1990s and few variations at the national scale. We also found some degree of local spatial structuring in western France. Overall, our results demonstrate the potential of large-scale sequence-based surveillance to monitor changes in the epidemiological situation of enzootic diseases.","PeriodicalId":23658,"journal":{"name":"Veterinary Research","volume":null,"pages":null},"PeriodicalIF":3.7,"publicationDate":"2024-10-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11451180/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142373048","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Biological characteristics of a new long-chain fatty acid transport protein 1 from Trichinella spiralis and its participation in lipid metabolism, larval moulting, and development. 一种新的螺旋毛霉长链脂肪酸转运蛋白 1 的生物学特征及其在脂质代谢、幼虫蜕皮和发育过程中的参与。

IF 3.7 1区农林科学

Veterinary Research Pub Date : 2024-09-30 DOI: 10.1186/s13567-024-01380-0

Yang Li Li, Qi Qi Lu, Wen Wen Zheng, Zhao Yu Zhang, Jin Yi Wu, Mei Hao Wei, Xin Zhuo Zhang, Ruo Dan Liu, Zhong Quan Wang, Jing Cui

{"title":"Biological characteristics of a new long-chain fatty acid transport protein 1 from Trichinella spiralis and its participation in lipid metabolism, larval moulting, and development.","authors":"Yang Li Li, Qi Qi Lu, Wen Wen Zheng, Zhao Yu Zhang, Jin Yi Wu, Mei Hao Wei, Xin Zhuo Zhang, Ruo Dan Liu, Zhong Quan Wang, Jing Cui","doi":"10.1186/s13567-024-01380-0","DOIUrl":"10.1186/s13567-024-01380-0","url":null,"abstract":"Long-chain fatty acid transport protein 1 (FATP1) is a member of the fatty acid transporter family. It facilitates transmembrane transport of fatty acids and participates in lipid metabolism. Lipids are essential components of the cell and organelle membranes of Trichinella spiralis. The nematode has lost the capacity to synthesise the necessary lipids de novo and has instead evolved to obtain fatty acids and their derivatives from its host. This study aims to ascertain the primary biological characteristics and roles of T. spiralis FATP1 (TsFATP1) in lipid metabolism, larval moulting, and the development of this nematode. The results show that TsFATP1 is highly expressed at enteral T. spiralis stages, mainly localised at the cuticle, the stichosome and the intrauterine embryos of the parasite. The silencing of the TsFATP1 gene by TsFATP1-specific dsRNA significantly decreases the expression levels of TsFATP1 in the worm. It reduces the contents of ATP, triglycerides, total cholesterol, and phospholipids both in vitro and in vivo. RNAi inhibits lipid metabolism, moulting, and the growth of this nematode. The results demonstrate that TsFATP1 plays an essential role in lipid metabolism, moulting, and the development of T. spiralis. It could also be a target candidate for the anti-Trichinella vaccine and drugs.","PeriodicalId":23658,"journal":{"name":"Veterinary Research","volume":null,"pages":null},"PeriodicalIF":3.7,"publicationDate":"2024-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11443915/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142354982","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Correction: Baicalin attenuates PD-1/PD-L1 axis-induced immunosuppression in piglets challenged with Glaesserella parasuis by inhibiting the PI3K/ Akt/mTOR and RAS/MEK/ERK signalling pathways. 更正：通过抑制 PI3K/ Akt/mTOR 和 RAS/MEK/ERK 信号通路，黄芩苷可减轻 PD-1/PD-L1 轴诱导的仔猪寄生虫疱疹病毒免疫抑制。

IF 3.7 1区农林科学

Veterinary Research Pub Date : 2024-09-30 DOI: 10.1186/s13567-024-01375-x

Shulin Fu, Jingyang Li, Jiarui You, Siyu Liu, Qiaoli Dong, Yunjian Fu, Ronghui Luo, Yamin Sun, Xinyue Tian, Wei Liu, Jingyi Zhang, Yu Ding, Yitian Zhang, Wutao Wang, Ling Guo, Yinsheng Qiu

引用次数: 0

Use of virus-like particles and nanoparticle-based vaccines for combating picornavirus infections. 利用病毒样颗粒和基于纳米颗粒的疫苗防治皮卡病毒感染。

IF 3.7 1区农林科学

Veterinary Research Pub Date : 2024-09-30 DOI: 10.1186/s13567-024-01383-x

Mei Ren, Sahibzada Waheed Abdullah, Chenchen Pei, Huichen Guo, Shiqi Sun

{"title":"Use of virus-like particles and nanoparticle-based vaccines for combating picornavirus infections.","authors":"Mei Ren, Sahibzada Waheed Abdullah, Chenchen Pei, Huichen Guo, Shiqi Sun","doi":"10.1186/s13567-024-01383-x","DOIUrl":"10.1186/s13567-024-01383-x","url":null,"abstract":"Picornaviridae are non-enveloped ssRNA viruses that cause diseases such as poliomyelitis, hand-foot-and-mouth disease (HFMD), hepatitis A, encephalitis, myocarditis, and foot-and-mouth disease (FMD). Virus-like particles (VLPs) vaccines mainly comprise particles formed through the self-assembly of viral capsid proteins (for enveloped viruses, envelope proteins are also an option). They do not contain the viral genome. On the other hand, the nanoparticles vaccine (NPs) is mainly composed of self-assembling biological proteins or nanomaterials, with viral antigens displayed on the surface. The presentation of viral antigens on these particles in a repetitive array can elicit a strong immune response in animals. VLPs and NPs can be powerful platforms for multivalent antigen presentation. This review summarises the development of virus-like particle vaccines (VLPs) and nanoparticle vaccines (NPs) against picornaviruses. By detailing the progress made in the fight against various picornaviruses such as poliovirus (PV), foot-and-mouth disease virus (FMDV), enterovirus (EV), Senecavirus A (SVA), and encephalomyocarditis virus (EMCV), we in turn highlight the significant strides made in vaccine technology. These advancements include diverse construction methods, expression systems, elicited immune responses, and the use of various adjuvants. We see promising prospects for the continued development and optimisation of VLPs and NPs vaccines. Future research should focus on enhancing these vaccines' immunogenicity, stability, and delivery methods. Moreover, expanding our understanding of the interplay between these vaccines and the immune system will be crucial. We hope these insights will inspire and guide fellow researchers in the ongoing quest to combat picornavirus infections more effectively.","PeriodicalId":23658,"journal":{"name":"Veterinary Research","volume":null,"pages":null},"PeriodicalIF":3.7,"publicationDate":"2024-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11443892/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142354993","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Hepatic transcriptomic analysis reveals differential regulation of metabolic and immune pathways in three strains of chickens with distinct growth rates exposed to mixed parasite infections. 肝脏转录组分析揭示了三种不同生长速度的鸡在受到混合寄生虫感染时对代谢和免疫途径的不同调控。

IF 3.7 1区农林科学

Veterinary Research Pub Date : 2024-09-28 DOI: 10.1186/s13567-024-01378-8

Oyekunle John Oladosu, Henry Reyer, Rosemarie Weikard, Beatrice Grafl, Dieter Liebhart, Cornelia C Metges, Christa Kühn, Gürbüz Daş

{"title":"Hepatic transcriptomic analysis reveals differential regulation of metabolic and immune pathways in three strains of chickens with distinct growth rates exposed to mixed parasite infections.","authors":"Oyekunle John Oladosu, Henry Reyer, Rosemarie Weikard, Beatrice Grafl, Dieter Liebhart, Cornelia C Metges, Christa Kühn, Gürbüz Daş","doi":"10.1186/s13567-024-01378-8","DOIUrl":"https://doi.org/10.1186/s13567-024-01378-8","url":null,"abstract":"During parasite infections, the liver may prioritise immune-related pathways over its metabolic functions. Intestinal infections caused by Ascaridia galli and Heterakis gallinarum impair feed intake, nutrient absorption, and weight gain. Histomonas meleagridis, vectored by H. gallinarum, can also damage liver tissues, potentially impairing liver functions. This study examined the hepatic gene expression in three strains of chickens: Ross-308 (R), Lohmann Brown Plus (LB), and Lohmann Dual (LD), 2 weeks after an experimental infection (n = 18) with both A. galli and H. gallinarum or kept as uninfected control (n = 12). Furthermore, H. gallinarum infection led to a co-infection with H. meleagridis. The mixed infections reduced feed intake and the average daily weight gain (P < 0.001). The infections also increased the plasma concentrations of alpha (1)-acid glycoprotein and the antibody titre against H. meleagridis (P = 0.049), with no strain differences (P > 0.05). For host molecular response, 1887 genes were differentially expressed in LD, while 275 and 25 genes were differentially expressed in R and LB, respectively. The up-regulated genes in R and LD were mostly related to inflammatory and adaptive immune responses, while down-regulated genes in LD were involved in metabolic pathways, including gluconeogenesis. Despite performance differences among the strains, worm burdens were similar, but hepatic molecular responses differed significantly. Moreover, there was an indication of a shift in hepatic functions towards immune-related pathways. We, therefore, conclude that the liver shifts its functions from metabolic to immune-related activities in chickens when challenged with mixed parasite species.","PeriodicalId":23658,"journal":{"name":"Veterinary Research","volume":null,"pages":null},"PeriodicalIF":3.7,"publicationDate":"2024-09-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11439216/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142354986","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

RSAD2 suppresses viral replication by interacting with the Senecavirus A 2 C protein. RSAD2 通过与塞内卡病毒 A 2 C 蛋白相互作用来抑制病毒复制。

IF 3.7 1区农林科学

Veterinary Research Pub Date : 2024-09-27 DOI: 10.1186/s13567-024-01370-2

Lei Hou, Zhi Wu, Penghui Zeng, Xiaoyu Yang, Yongyan Shi, Jinshuo Guo, Jianwei Zhou, Jiangwei Song, Jue Liu

{"title":"RSAD2 suppresses viral replication by interacting with the Senecavirus A 2 C protein.","authors":"Lei Hou, Zhi Wu, Penghui Zeng, Xiaoyu Yang, Yongyan Shi, Jinshuo Guo, Jianwei Zhou, Jiangwei Song, Jue Liu","doi":"10.1186/s13567-024-01370-2","DOIUrl":"10.1186/s13567-024-01370-2","url":null,"abstract":"Senecavirus A (SVA), an emerging virus that causes blisters on the nose and hooves, reduces the production performance of pigs. RSAD2 is a radical S-adenosylmethionine (SAM) enzyme, and its expression can suppress various viruses due to its broad antiviral activity. However, the regulatory relationship between SVA and RSAD2 and the mechanism of action remain unclear. Here, we demonstrated that SVA infection increased RSAD2 mRNA levels, whereas RSAD2 expression negatively regulated viral replication, as evidenced by decreased viral VP1 protein expression, viral titres, and infected cell numbers. Viral proteins that interact with RSAD2 were screened, and the interaction between the 2 C protein and RSAD2 was found to be stronger than that between other proteins. Additionally, amino acids (aa) 43-70 of RSAD2 were crucial for interacting with the 2 C protein and played an important role in its anti-SVA activity. RSAD2 was induced by type I interferon (IFN-I) via Janus kinase signal transducer and activator of transcription (JAK-STAT), and had antiviral activity. Ruxolitinib, a JAK-STAT pathway inhibitor, and the knockdown of JAK1 expression substantially reduced RSAD2 expression levels and antiviral activity. Taken together, these results revealed that RSAD2 blocked SVA infection by interacting with the viral 2 C protein and provide a strategy for preventing and controlling SVA infection.","PeriodicalId":23658,"journal":{"name":"Veterinary Research","volume":null,"pages":null},"PeriodicalIF":3.7,"publicationDate":"2024-09-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11430333/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142354992","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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