Veterinary Research最新文献

{"title":"Description of a contemporary pathogenic Escherichia coli isolated from pigs with post-weaning diarrhea in the United States from 2010 to 2023.","authors":"Rodrigo C Paiva, Eric R Burrough, Nubia Macedo, Ana Paula S P Silva, Maud de Lagarde, John M Fairbrother, Pablo E Piñeiro, Marcelo N Almeida","doi":"10.1186/s13567-025-01568-y","DOIUrl":"10.1186/s13567-025-01568-y","url":null,"abstract":"<p><p>Post-weaning diarrhea (PWD) due to Escherichia coli in pigs is a significant enteric disease in the U.S. Contemporary data about the main virulence factors, colony morphology, and distribution of virotypes of isolates associated with post-weaning colibacillosis (PWC) is essential information for swine veterinarians, producers, and stakeholders. This study reports the rate of PWC/PWD, frequency of detection of fimbrial types, pathotypes, toxins, virotypes, morphological characteristics, and temporal analysis of the most prevalent virotype of enterotoxigenic E. coli recovered from cases of PWD in pigs in the United States. There was a significant increase in PWC cases submitted to the diagnostic laboratory in 2013, 2014, and 2021, respectively. A greater frequency of detection of F18 fimbrial adhesin (69.87%) was observed compared to F4 (26.19%), F5 (0.25%), F41 (0.13%), AIDA (0.10%), and multiple adhesins (3.46%), respectively. Hybrid ETEC:STEC pathotype was greater than ETEC and STEC, and STb toxin was present in 93.33% of the detected isolates. The most frequently observed virotype was F18:LT:STa:STb:Stx2e (27.71%). Smooth:mucoid colony morphology was associated with a greater likelihood of PWC (0.758, 0.968, and 0.993) compared to smooth, intermediate, and rough, respectively. The frequency of the F18:LT:STa:STb:Stx2e virotype increased across all U.S. states from 2016 to 2023, greatest in the northwest and east of Iowa and northeast Indiana in 2022 and Iowa in 2023. The virulence factors and morphology of enterotoxigenic E. coli associated with PWC across different states in the U.S. from 2010 to 2023 were diverse; nevertheless, the virotype F18:LT:STa:STb:Stx2e predominated and increased in frequency during this time period.</p>","PeriodicalId":23658,"journal":{"name":"Veterinary Research","volume":"56 1","pages":"130"},"PeriodicalIF":3.7,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12218006/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144545050","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"IDO1 promotes Echinococcus multilocularis infection by regulating the formation of neutrophil extracellular traps.","authors":"Haining Zhang, Ru Meng, Fan Zhang, Ao Chen, Hongrun Ge, Wangkai Chen, Zhi Li, Yong Fu","doi":"10.1186/s13567-025-01572-2","DOIUrl":"10.1186/s13567-025-01572-2","url":null,"abstract":"<p><p>The widespread prevalence of alveolar echinococcosis (AE) caused by Echinococcus multilocularis infection poses a significant threat to human health. E. multilocularis is found primarily in the Northern Hemisphere. Given the limitations of current treatment methods, primarily surgical resection, there is a pressing need for more effective therapeutic options. We established a mouse model of E. multilocularis infection by injecting E. multilocularis protoscoleces into C57BL/6 mice. The formation of neutrophil extracellular traps (NETs) following E. multilocularis infection was identified and validated using various techniques, including transcriptome sequencing, scanning electron microscopy (SEM) and flow cytometry. We found that the knockout of the Indoleamine 2, 3 dioxygenase 1 (IDO1) gene or the administration of IDO1 inhibitors resulted in a decrease in the levels of NET-related molecules, including CitH3, MPO, PAD4, PR3, NE, and MPO-DNA complexes, as well as cfDNA. In addition, after the addition of the IDO1 inhibitor, the levels of p65, phospho-p65, p50/105, and REL increased. These results showed that IDO1 promotes the formation of NETs and inhibits NF-κB activation. Moreover, IDO1 inhibits AE progression by regulating NET formation. In conclusion, this study revealed that IDO1 inhibits AE progression by regulating NET formation, and this regulation may be associated with IDO1-induced neutrophil production and NF-κB signalling activation. These results are valuable for understanding the pathogenesis of E. multilocularis and may offer new insights for the prevention and treatment of AE.</p>","PeriodicalId":23658,"journal":{"name":"Veterinary Research","volume":"56 1","pages":"131"},"PeriodicalIF":3.7,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12217305/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144545055","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genomic characterization and virulence of Streptococcus suis serotype 7 sequence type 373 of clonal complex 94.","authors":"Rujirat Hatrongjit, Parichart Boueroy, Peechanika Chopjitt, Thidathip Wongsurawat, Piroon Jenjaroenpun, Natnicha Wankaew, Zeren Peng, Han Zheng, Marcelo Gottschalk, Zongfu Wu, Anusak Kerdsin","doi":"10.1186/s13567-025-01562-4","DOIUrl":"10.1186/s13567-025-01562-4","url":null,"abstract":"<p><p>Streptococcus suis is a swine pathogen that also causes invasive infections in humans, leading to significant economic losses in pig production worldwide. Serotype 2 is the most pathogenic S. suis strain associated with human infections. However, non-serotype 2 strains isolated from humans have been reported globally. Here, we conducted a comparative genomic analysis of S. suis serotype 7-ST373 strains belonging to clonal complex 94, isolated from both humans and pigs, and assessed their virulence through mouse infection experiments. Genomic analysis revealed that S. suis serotype 7-ST373 strains harbor genomic islands 1-3 of the pathogenic S. suis clade. They also possess a high number of virulence-associated genes, similar to those of virulent serotype 2 strains, suggesting a high virulence potential. The antimicrobial resistance gene tet(O), which confers tetracycline resistance, was found in all ST373 strains, while erm(B), which confers macrolide resistance, was detected in most ST373 strains. However, the macrolide and lincosamide resistance genes lnu(B) and lsa(E) were found exclusively in a Thai human strain. Comparative genomics of ST373 strains with the virulent serotype 2 strain P1/7 identified 76 unique genes in ST373 strains, including 30 genes exclusively present in human ST373 strains. Mouse virulence experiments with two human ST373 strains (GX69 and STC2826) and one strain from a healthy pig (WUSS318) resulted in 100% mortality, classifying them as highly virulent. These findings indicate that serotype 7-ST373 strains demonstrate pathogenic potential and should be closely monitored.</p>","PeriodicalId":23658,"journal":{"name":"Veterinary Research","volume":"56 1","pages":"125"},"PeriodicalIF":3.7,"publicationDate":"2025-06-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12186334/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144476904","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"CD44 mediates the internalization of foot-and-mouth disease virus through macropinocytosis.","authors":"Xuefei Wang, Hu Dong, Jin'en Wu, Yun Zhang, Shiqi Sun, Huichen Guo","doi":"10.1186/s13567-025-01555-3","DOIUrl":"10.1186/s13567-025-01555-3","url":null,"abstract":"<p><p>Foot-and-mouth disease virus (FMDV), a member of the Picornavirus family, poses a significant threat to global animal husbandry. While it is known that macropinocytosis plays a crucial role in the entry of FMDV into cells, the specific cellular proteins that regulate this process and the downstream signalling pathways associated with these proteins are not yet fully understood. In this study, we demonstrated that the membrane protein cluster of differentiation 44 (CD44) is essential for the internalization of FMDV in BHK-21 cells, while it is not involved in the attachment of the virus to the cell. In addition, we found that CD44 is internalized in response to FMDV entry. Interestingly, the use of a macropinocytosis inhibitor impaired both CD44 internalization and FMDV entry and infection, indicating a strong connection between these two processes. Further experiments revealed that CD44 facilitates FMDV internalization through macropinocytosis. Importantly, our study shows that CD44 interacts with FMDV capsid proteins VP2 and VP3 and becomes phosphorylated during the entry of the virus. The phosphorylation of CD44 subsequently promotes the phosphorylation of p21-activated kinase 1 (PAK1), which is a critical component in the macropinocytotic entry of FMDV. Overall, this study provides new insights into the role of CD44 in the invasion of pathogens into host cells and highlights potential strategies for improving FMDV vaccines.</p>","PeriodicalId":23658,"journal":{"name":"Veterinary Research","volume":"56 1","pages":"123"},"PeriodicalIF":3.7,"publicationDate":"2025-06-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12181885/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144340464","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mesomycoplasma hyopneumoniae lipoprotein Mhp390 serves as a plasminogen receptor mediating extracellular matrix degradation and respiratory epithelial cells injury.","authors":"Wei Liu, Liying Ye, Keli Yang, Xingyu Yan, Ting Gao, Fangyan Yuan, Rui Guo, Zewen Liu, Chang Li, Qiong Wu, Jiajia Zhu, Yongxiang Tian, Bo Tang, Qiqi Song, Danna Zhou","doi":"10.1186/s13567-025-01551-7","DOIUrl":"10.1186/s13567-025-01551-7","url":null,"abstract":"<p><p>The destruction of the respiratory barrier caused by Mesomycoplasma (Mycoplasma) hyopneumoniae plays a pivotal role in facilitating secondary infections by other respiratory pathogens. However, the pathogenesis of M. hyopneumoniae breaching the respiratory barrier to establish infection remains largely elusive. In this study, the role of Mhp390 encoded by M. hyopneumoniae in invasion of the respiratory tract barrier, including extracellular matrix and tracheal epithelial cells, were investigated through the Transwell assay. Our finding indicated that M. hyopneumoniae may exploit the host fibrinolytic system via Mhp390 to accumulate activated plasmin outside its membrane, thereby breaching the respiratory tract barrier and facilitating the progression of infection. Furthermore, the key functional domains within Mhp390 involved in its interaction with host plasminogen were determined by using truncated mutation techniques. Collectively, these findings will enhance our understanding of the mechanism underlying respiratory barrier invasion by M. hyopneumoniae thereby providing new theoretical basis for the development of novel vaccines and effective control strategies against secondary infection.</p>","PeriodicalId":23658,"journal":{"name":"Veterinary Research","volume":"56 1","pages":"124"},"PeriodicalIF":3.7,"publicationDate":"2025-06-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12182672/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144340465","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correction: Serotype diversity of Actinobacillus pleuropneumoniae detected by real-time PCR in clinical and subclinical samples from Spanish pig farms during 2017-2022.","authors":"José Luis Arnal Bernal, Marcelo Gottschalk, Sonia Lacotoure, Celia Sanz Tejero, Gema Chacón Pérez, Desiree Martín-Jurado, Ana Belén Fernández Ros","doi":"10.1186/s13567-025-01564-2","DOIUrl":"10.1186/s13567-025-01564-2","url":null,"abstract":"","PeriodicalId":23658,"journal":{"name":"Veterinary Research","volume":"56 1","pages":"122"},"PeriodicalIF":3.7,"publicationDate":"2025-06-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12181848/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144337076","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"LGALS3BP/90K suppresses porcine reproductive and respiratory syndrome virus replication by enhancing GP3 degradation and stimulating innate immunity.","authors":"Qingyu Fan, Haiyan Wang, Hanze Liu, Jinzhi Li, Juan Huang, Ying Yu, Zhi Cao, Qiaoya Zhang","doi":"10.1186/s13567-025-01556-2","DOIUrl":"10.1186/s13567-025-01556-2","url":null,"abstract":"<p><p>Porcine reproductive and respiratory syndrome virus (PRRSV) seriously affects the world pig industry, and existing vaccines are not enough to protect against PRRSV efficiently. Identifying host factors that inhibit PRRSV infection may provide potential targets for antiviral therapies. Galectin 3 binding protein (LGALS3BP), also referred to as 90K, is a multifunctional protein that plays significant roles in viral infections, but its role in PRRSV infection is not clearly defined. In this study, we found that 90K expression increased during PRRSV infection. Additionally, 90K inhibits PRRSV replication by restricting both viral RNA synthesis and viral assembly. Further studies showed that 90K can interact with various PRRSV structural proteins, such as GP2, GP3, and GP5. Moreover, 90K targets K217 in GP3 for K48-linked ubiquitination, facilitating GP3 degradation through the proteasomal pathway. Meanwhile, we found that 90K induces the expression of type I interferon (IFN) and inflammatory cytokines by activating NF-κB and IRF3. Overall, our findings suggest that 90K plays a negative role in PRRSV replication by inducing PRRSV-GP3 degradation and activating innate immune responses.</p>","PeriodicalId":23658,"journal":{"name":"Veterinary Research","volume":"56 1","pages":"121"},"PeriodicalIF":3.7,"publicationDate":"2025-06-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12180180/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144337077","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Assessing the efficiency of the bovine brucellosis surveillance-control system in a disease-free context through agent-based modelling.","authors":"Sofia Mlala, Sébastien Picault, Carole Sala, Pierre Villard, Jean-Luc Vinard, Viviane Hénaux","doi":"10.1186/s13567-025-01549-1","DOIUrl":"10.1186/s13567-025-01549-1","url":null,"abstract":"<p><p>In France, bovine brucellosis is subject to stringent surveillance to ensure early detection of re-emergence and maintain the country's disease-free status. However, the cost-efficiency of such surveillance systems has rarely been assessed. This study aimed to: (1) evaluate the detection delay of bovine brucellosis for current and alternative surveillance systems following its reintroduction; and (2) estimate the associated surveillance and control costs. A mechanistic, stochastic, agent-based simulation model was developed to represent the spread of bovine brucellosis within and between cattle farms in mainland France after the introduction of an infected animal. The results showed: (1) improved efficiency (considering both effectiveness and cost) when suspending testing at introduction; (2) reduced efficiency when annually screening all adults in one-third of suckler herds compared to 20% of adults in all suckler herds; and (3) slightly improved efficiency when reporting abortion series instead of all individual cases. The current surveillance system detected infection within a median delay of 49-51 weeks, with a median of one infected farm at confirmation, regardless of the epidemiological context. For alternative systems, detection occurred within a median of 40-99 weeks, with one to three infected farms. These findings suggest that, under model assumptions, all surveillance systems maintained a relatively low number of infected herds. Our study provides cost-effectiveness information that will help stakeholders compare the efficiency of alternative scenarios, improve the current surveillance system and make relevant decisions about the allocations of resources.</p>","PeriodicalId":23658,"journal":{"name":"Veterinary Research","volume":"56 1","pages":"120"},"PeriodicalIF":3.7,"publicationDate":"2025-06-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12172338/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144318110","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The pathogenesis of foot-and-mouth disease virus: current understandings and knowledge gaps.","authors":"Carolina Stenfeldt, Michael Eschbaumer, John Humphreys, Gisselle N Medina, Jonathan Arzt","doi":"10.1186/s13567-025-01545-5","DOIUrl":"10.1186/s13567-025-01545-5","url":null,"abstract":"<p><p>Foot-and-mouth disease (FMD) continues to be one of the most important diseases of livestock globally based upon both biological features and regulatory aspects. Few pathogens have had comparable impact on global livestock production and regulation of international trade in animal-derived products. The pathogenesis (interaction between pathogen and host) is central to the importance of the disease ranging from how the causal pathogen, FMD virus (FMDV), transmits between hosts and is maintained in populations. Key accomplishments over the last decade include description of the primary sites of infection in domestic species, delineating critical differences in temporo-anatomic progression in different host species and emphasizing that knowledge gained regarding FMDV pathogenesis in one host cannot necessarily be extrapolated and applied to a different host. Host responses to infection and viral genomics have been characterized with ever-increasing granularity. Yet, the numerous knowledge gaps that remain in understanding FMDV pathogenesis impede advancements in FMD control and eradication. For instance, it remains unclear if long-term asymptomatic FMDV carriers are biologically relevant (contagious) and the manner in which host genomics and transcriptomics affect pathogenesis during different phases of infection. The characterization of neoteric subclinical infection as a disease stage that is distinct from the persistent \"FMDV carrier state\" has emphasized the importance of sample collection from clinically unaffected animals for FMDV surveillance. Similarly, incorporating a phase of pre-clinical infectiousness in simulation modeling can dramatically improve prediction of FMD outbreaks in non-endemic regions. The outcome of FMDV infection with regards to viral persistence differs between host species as well as between individuals of the same species. Yet, we lack a satisfactory explanation of the host factors that drive the FMDV carrier state divergence. This review was based upon a gap-analysis workshop organized by the Global Foot-and-Mouth Disease Research Alliance (GFRA) in Buenos Aires, Argentina, in December of 2022. The purpose of this work is to summarize the current understanding of the distinct compartments of FMD pathogenesis with an emphasis on progress made within the last decade and present the critical knowledge gaps that continue to limit FMD control and eradication.</p>","PeriodicalId":23658,"journal":{"name":"Veterinary Research","volume":"56 1","pages":"119"},"PeriodicalIF":3.7,"publicationDate":"2025-06-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12172366/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144310505","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genetic characterization and pathogenicity analysis of three porcine epidemic diarrhea virus strains isolated from North China.","authors":"Ying Liu, Jinghui Fan, Wenyuan Gu, Yunhuan Zhao, Shuai Zhang, Yuzhu Zuo","doi":"10.1186/s13567-025-01554-4","DOIUrl":"10.1186/s13567-025-01554-4","url":null,"abstract":"<p><p>Porcine epidemic diarrhea (PED) is a highly contagious intestinal disease owing to porcine epidemic diarrhea virus (PEDV) infection. It is extremely detrimental to newborn piglets and has caused huge economic losses to the global pig industry. In this study, three PEDV strains of G2a PEDV-WF/2023, G2b PEDV-SX/2024 and PEDV-HS/2024 were successfully isolated from small intestine tissue samples with the analysis of their molecular structure characteristics, genetic characteristics and pathogenicity. Notably, these three PEDV strains had multiple unique aa mutations and extensive N-glycosylation in the D0 region, S1-NTD, COE epitope and SS6, respectively. Therefore, their structures were different compared to CV777 and PT-P5 strains. Furthermore, all the three PEDV strains caused severe clinical symptoms in 1-day-old piglets after infection. Among them, G2a PEDV-WF/2023 was the most detrimental to piglets, with highly levels of viral RNA in vivo. In contrast, PEDV-HS/2024 showed relatively weak pathogenicity to piglets, but it also caused the death of piglets. It might be attributed to the occurrence of individual mutations consistent with the amino acid sequence of G1b subtype in PEDV-HS/2024 strain. Findings in this study allow us to confirm that the G2a PEDV-WF/2023 strain is currently one of the most harmful epidemic strains to piglets. This study may benefit our understanding of the molecular structure characteristics, evolution trend and transmission dynamics of the epidemic strains in China. Moreover, it may provide potential reference for formulating more targeted PEDV vaccines, preventing and controlling this infection, and further curbing the cross-species spread of PEDV.</p>","PeriodicalId":23658,"journal":{"name":"Veterinary Research","volume":"56 1","pages":"118"},"PeriodicalIF":3.7,"publicationDate":"2025-06-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12166606/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144294984","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}