Veterinary Research最新文献_第9页

Inhibition of STING-mediated type I IFN signaling by African swine fever virus DP71L. 非洲猪瘟病毒 DP71L 对 STING 介导的 I 型 IFN 信号传导的抑制。

IF 3.7 1区农林科学

Veterinary Research Pub Date : 2025-02-04 DOI: 10.1186/s13567-025-01474-3

Lakmal Ranathunga, Sachini Abesinghe, Ji-Won Cha, Niranjan Dodantenna, Kiramage Chathuranga, Asela Weerawardhana, D K Haluwana, Nuwan Gamage, Jong-Soo Lee

{"title":"Inhibition of STING-mediated type I IFN signaling by African swine fever virus DP71L.","authors":"Lakmal Ranathunga, Sachini Abesinghe, Ji-Won Cha, Niranjan Dodantenna, Kiramage Chathuranga, Asela Weerawardhana, D K Haluwana, Nuwan Gamage, Jong-Soo Lee","doi":"10.1186/s13567-025-01474-3","DOIUrl":"10.1186/s13567-025-01474-3","url":null,"abstract":"African swine fever virus (ASFV) is nucleocytoplasmic large DNA arbovirus and encodes many proteins involved in the interaction with host molecules to evade antiviral immune responses. Especially, evasion strategies of type I interferon (IFN-I)-mediated immune responses are crucial for early ASFV replication. However, there is still a lack of information regarding the immune evasion mechanism of ASFV proteins. Here, we demonstrated that ASFV DP71L suppresses STING-mediated antiviral responses. The conserved phosphatase 1 (PP1) motif of DP71L specifically interact with the C-terminal tail (CTT) of STING and in particular, amino acids P371, L374, and R375 of STING were important for interaction with DP71L. Consequently, this interaction disrupted the binding between STING and TANK-binding kinase 1 (TBK1), thereby inhibiting downstream signaling including phosphorylation of TBK1, STING and IRF3 for antiviral signaling. DP71L significantly interfered with viral DNA induced interferon production and IFN-mediated downstream signaling in vitro. Consistently, knockdown of DP71L enhanced antiviral gene expression in ASFV-infected cells. Taken together, these results highlight the important role of DP71L with respect to inhibition of interferon responses and provide guidance for a better understanding of ASFV pathogenesis and the development of live attenuated ASFV vaccines.","PeriodicalId":23658,"journal":{"name":"Veterinary Research","volume":"56 1","pages":"27"},"PeriodicalIF":3.7,"publicationDate":"2025-02-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11796124/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143190535","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

MyD88 inhibitor TJ-M2010-5 alleviates spleen impairment and inflammation by inhibiting the PI3K/miR-136-5p/AKT3 pathway in the early infection of Trichinella spiralis. MyD88抑制剂TJ-M2010-5通过抑制旋毛虫感染早期的PI3K/miR-136-5p/AKT3通路，减轻脾脏损伤和炎症。

IF 3.7 1区农林科学

Veterinary Research Pub Date : 2025-02-04 DOI: 10.1186/s13567-025-01459-2

Huifang Bai, Qianqian Dang, Guoliang Chen, Lingfeng Xie, Saining Wang, Ning Jiang, Xiaoxia Wu, Shuyan Zhang, Xuelin Wang

{"title":"MyD88 inhibitor TJ-M2010-5 alleviates spleen impairment and inflammation by inhibiting the PI3K/miR-136-5p/AKT3 pathway in the early infection of Trichinella spiralis.","authors":"Huifang Bai, Qianqian Dang, Guoliang Chen, Lingfeng Xie, Saining Wang, Ning Jiang, Xiaoxia Wu, Shuyan Zhang, Xuelin Wang","doi":"10.1186/s13567-025-01459-2","DOIUrl":"10.1186/s13567-025-01459-2","url":null,"abstract":"Trichinella spiralis (T. spiralis) has been reported to induce inflammation, which can cause immune system dysregulation. Myeloid differentiation primary response gene 88 (MyD88) is implicated in inflammation signalling pathways. TJ-M2010-5 is a novel MyD88 inhibitor with remarkable protective effects against several diseases. However, the precise mechanism of TJ-M2010-5's involvement in spleen impairment and inflammation in the early infection of T. spiralis has yet to be fully elucidated. This study analysed histological, inflammation, and macrophage polarisation of the early T. spiralis-infected mice treated with TJ-M2010-5. MyD88 promoter methylation results showed that the methylation levels in the 5 d group were lower compared to the control group (P < 0.05). Furthermore, the methylation led to an imbalance in anti-inflammatory regulation in the infected mice. After TJ-M2010-5 treatment, spleen impairment was reduced. Sequencing analysis showed that TJ-M2010-5 significantly up-regulated 9 and down-regulated 10 miRNAs compared with the 5 d group. A dual-luciferase reporter assay further revealed that miR-136-5p is involved in the TJ-M2010-5 treatment by targeting AKT3. In RAW264.7 cells, TJ-M2010-5 pre-treatment significantly reversed the M1 polarisation and inhibited nitric oxide (NO) production. LC-MS/MS results showed TJ-M2010-5 was hepatosplenic-targeted. In conclusion, the study demonstrates that TJ-M2010-5 could effectively alleviate spleen impairment and reduce inflammation in mice infected with T. spiralis in its early stages by blocking the activation of PI3K/miR-136-5p/AKT3.","PeriodicalId":23658,"journal":{"name":"Veterinary Research","volume":"56 1","pages":"28"},"PeriodicalIF":3.7,"publicationDate":"2025-02-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11796171/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143190539","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Lawsonia intracellularis T3SS effector LI0758, an Rce1 ortholog, activates MAPK and NF-κB signaling in mammalian cells. 胞内Lawsonia intracellularis T3SS效应物LI0758是Rce1同源物，可激活哺乳动物细胞中的MAPK和NF-κB信号。

IF 3.7 1区农林科学

Veterinary Research Pub Date : 2025-02-04 DOI: 10.1186/s13567-025-01461-8

Yuanxiu Zhong, Yiyun Duan, Fenju Lai, Jinhua Zhang, Yimin Dai

{"title":"Lawsonia intracellularis T3SS effector LI0758, an Rce1 ortholog, activates MAPK and NF-κB signaling in mammalian cells.","authors":"Yuanxiu Zhong, Yiyun Duan, Fenju Lai, Jinhua Zhang, Yimin Dai","doi":"10.1186/s13567-025-01461-8","DOIUrl":"10.1186/s13567-025-01461-8","url":null,"abstract":"Lawsonia intracellularis, a Gram-negative obligate intracellular bacterium causing porcine proliferative enteropathy, possesses a type III secretion system (T3SS), yet only a handful of its substrates have been experimentally characterized. In this study, we identify that LI0758 can be secreted by the Yersinia T3SS, which suppresses yeast growth and activates mitogen-activated protein kinase (MAPK) and nuclear factor-kappa B (NF-κB) signaling pathway in mammalian cells. Bioinformatics analyses indicate that LI0758 is an ortholog of Rce1, a eukaryotic CAAX protein endoprotease, sharing a similar subcellular localization on the endoplasmic reticulum (ER). While displaying unique activity in the yeast a-factor reporter system, LI0758 restores Ras2 localization in Rce1Δ mutant strains, implying functional similarity. Our findings underscore LI0758's pivotal role in activating MAPK pathways and suggest its potential to modulate the localization and function of host CAAX proteins. Further investigation holds promise for elucidating novel bacteria-host interaction mechanisms and fostering the development of innovative therapies against proliferative enteritis.","PeriodicalId":23658,"journal":{"name":"Veterinary Research","volume":"56 1","pages":"29"},"PeriodicalIF":3.7,"publicationDate":"2025-02-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11796264/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143190537","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Targeting AI-2 quorum sensing: harnessing natural products against Streptococcus suis biofilm infection. 针对AI-2群体感应：利用天然产物对抗猪链球菌生物膜感染。

IF 3.7 1区农林科学

Veterinary Research Pub Date : 2025-02-04 DOI: 10.1186/s13567-025-01450-x

Shuji Gao, Shuo Yuan, Yingying Quan, Wenjie Jin, Yamin Shen, Rishun Li, Baobao Liu, Yuxin Wang, Li Yi, Shaohui Wang, Xiaogai Hou, Yang Wang

{"title":"Targeting AI-2 quorum sensing: harnessing natural products against Streptococcus suis biofilm infection.","authors":"Shuji Gao, Shuo Yuan, Yingying Quan, Wenjie Jin, Yamin Shen, Rishun Li, Baobao Liu, Yuxin Wang, Li Yi, Shaohui Wang, Xiaogai Hou, Yang Wang","doi":"10.1186/s13567-025-01450-x","DOIUrl":"10.1186/s13567-025-01450-x","url":null,"abstract":"The biofilm acts as a protective layer for Streptococcus suis (S. suis), contributing to the development of drug resistance and chronic infections. Autoinducer 2 (AI-2) quorum sensing represents the primary regulatory pathway governing biofilm formation in S. suis. Consequently, targeting AI-2 quorum sensing to inhibit biofilm formation represents a promising strategy for preventing and managing drug resistance and chronic infections caused by S. suis. This study established a small natural product library by integrating commercial drug molecules with Chinese herbal medicine molecules. Consequently, two natural products, salvianolic acid A (SAA) and rhapontin (RH), which target S. suis AI-2 via quorum sensing, were identified. SAA and RH inhibit AI-2 synthesis through noncompetitive and competitive binding to S-ribosylhomocysteinase (LuxS). By inhibiting S. suis AI-2 quorum sensing, these compounds modulate the expression of adhesion genes and the synthesis of extracellular polysaccharides (EPS), reducing the adhesion ability of S. suis and ultimately inhibiting biofilm formation. Using LC‒MS/MS, we further analysed the impact of SAA and RH on the metabolic activity of S. suis, revealing the potential medicinal value of these compounds. Finally, the efficacy of SAA and RH against S. suis infection was validated in Galleria mellonella larvae, confirming their significant anti-infection effects.","PeriodicalId":23658,"journal":{"name":"Veterinary Research","volume":"56 1","pages":"26"},"PeriodicalIF":3.7,"publicationDate":"2025-02-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11796197/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143190544","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The cholesterol metabolite 25-hydroxycholesterol suppresses porcine deltacoronavirus via lipophagy inhibition and mTORC1 modulation. 胆固醇代谢产物25-羟基胆固醇通过脂噬抑制和mTORC1调节抑制猪冠状病毒。

IF 3.7 1区农林科学

Veterinary Research Pub Date : 2025-01-31 DOI: 10.1186/s13567-025-01452-9

Jia-Lu Zhang, Xue-Fei Wang, Jia-Lin Li, Cong Duan, Jiu-Feng Wang

{"title":"The cholesterol metabolite 25-hydroxycholesterol suppresses porcine deltacoronavirus via lipophagy inhibition and mTORC1 modulation.","authors":"Jia-Lu Zhang, Xue-Fei Wang, Jia-Lin Li, Cong Duan, Jiu-Feng Wang","doi":"10.1186/s13567-025-01452-9","DOIUrl":"10.1186/s13567-025-01452-9","url":null,"abstract":"25-Hydroxycholesterol (25HC) is a hydroxylated cholesterol with multiple antiviral activities, however, little is known about the mechanisms by which 25HC correlates antiviral ability with lipid droplet (LD) dynamic balance to ensure cholesterol homeostasis. In the present study, 25HC was applied to porcine deltacoronavirus (PDCoV)-infected LLC-PK1 (Lilly Laboratories Culture-Porcine Kidney 1) cells and piglets to explore its antiviral capacity and underlying mechanism. The results revealed that 25HC decreased free cholesterol (FC) levels but increased triglyceride (TG) levels in PDCoV-infected cells and piglets. The accumulation of LDs induced by oleic acid (OA) impedes PDCoV replication. In addition, 25HC administration increases LD accumulation and declines protein expression associated with lipophagy and lysosomes to facilitate LD accumulation. Moreover, 25HC inhibited TFEB (transcription factor-EB) expression, blocked its translocation into the nucleus and reversed Mechanistic Target of Rapamycin Complex 1 (mTORC1) activity, which in turn hindered lipophagy and PDCoV replication. Additionally, 25HC treatment ameliorated the clinical symptoms and intestinal injury of PDCoV-infected piglets. These findings reveal the beneficial effect of lipophagy on PDCoV infection and uncover the antiviral mechanism of 25HC, by which lipophagy and mTOR activity are tightly controlled by 25HC.","PeriodicalId":23658,"journal":{"name":"Veterinary Research","volume":"56 1","pages":"23"},"PeriodicalIF":3.7,"publicationDate":"2025-01-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11786589/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143075797","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Spray vaccination with a safe and bivalent H9N2 recombinant chimeric NDV vector vaccine elicits complete protection against NDV and H9N2 AIV challenge. 用安全的二价H9N2重组嵌合NDV载体疫苗喷雾接种，可对NDV和H9N2 AIV的攻击产生完全的保护作用。

IF 3.7 1区农林科学

Veterinary Research Pub Date : 2025-01-31 DOI: 10.1186/s13567-025-01448-5

Xiaoquan Wang, Jing Dai, Wenhao Yang, Yao Yao, Jin Zhang, Kaituo Liu, Xiaolong Lu, Ruyi Gao, Yu Chen, Jiao Hu, Min Gu, Shunlin Hu, Xiufan Liu, Xiaowen Liu

{"title":"Spray vaccination with a safe and bivalent H9N2 recombinant chimeric NDV vector vaccine elicits complete protection against NDV and H9N2 AIV challenge.","authors":"Xiaoquan Wang, Jing Dai, Wenhao Yang, Yao Yao, Jin Zhang, Kaituo Liu, Xiaolong Lu, Ruyi Gao, Yu Chen, Jiao Hu, Min Gu, Shunlin Hu, Xiufan Liu, Xiaowen Liu","doi":"10.1186/s13567-025-01448-5","DOIUrl":"10.1186/s13567-025-01448-5","url":null,"abstract":"Newcastle disease virus (NDV) and H9N2 avian influenza virus (AIV) represent significant pathogenic risks to the poultry industry, leading to considerable economic losses. Vaccination is a widely used preventive measure against these pathogens, yet the lack of a live bivalent vaccine targeting NDV and H9N2 AIV imposes a heavy vaccination burden. Previously, we constructed a genotype-matched chimeric NDV vector, LX-OAI4S, in which the genotype I NDV backbone was replaced with the ectodomain of haemagglutinin-neuraminidase (HN) and modified using the attenuated F gene from the genotype VII vaccine strain A-VII. Based on the LX-OAI4S vector, we successfully generated three H9N2 recombinant viruses: LX-OAI4S-NPU-HA, LX-OAI4S-MU-HA, and LX-OAI4S-HNU-HA. These recombinants incorporated the H9N2 HA gene, flanked by untranslated regions (UTRs) from the NP, M, or HN gene of the NDV LX strain, inserted between the P and M genes of LX-OAI4S. The vaccine candidate LX-OAI4S-NPU-HA induced a more robust immune response in chickens against H9N2 AIV and NDV than the other two recombinants. This response effectively protects against virus shedding and lethal virus challenge. Furthermore, spray vaccination with LX-OAI4S-NPU-HA showed protective efficacy against H9N2 AIV and NDV. This study offers a promising strategy for comprehensive protection in regions threatened by H9N2 AIV and NDV.","PeriodicalId":23658,"journal":{"name":"Veterinary Research","volume":"56 1","pages":"24"},"PeriodicalIF":3.7,"publicationDate":"2025-01-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11786375/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143075796","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Lactoferrin exhibits PEDV antiviral activity by interfering with spike-heparan sulfate proteoglycans binding and activating mucosal immune response. 乳铁蛋白通过干扰硫酸乙酰肝素蛋白聚糖结合和激活粘膜免疫反应来显示PEDV抗病毒活性。

IF 3.7 1区农林科学

Veterinary Research Pub Date : 2025-01-31 DOI: 10.1186/s13567-025-01456-5

Peng Liu, Jinjiao Zuo, Hui Lu, Bin Zhang, Caihong Wu

{"title":"Lactoferrin exhibits PEDV antiviral activity by interfering with spike-heparan sulfate proteoglycans binding and activating mucosal immune response.","authors":"Peng Liu, Jinjiao Zuo, Hui Lu, Bin Zhang, Caihong Wu","doi":"10.1186/s13567-025-01456-5","DOIUrl":"10.1186/s13567-025-01456-5","url":null,"abstract":"Neonatal piglets infected with Porcine Epidemic Diarrhea Virus (PEDV) experience a mortality rate of up to 90%, resulting in significant economic losses to the swine industry in China. Current strategies using specific antibodies in sow milk to prevent Porcine Epidemic Diarrhea (PED) in these piglets through specific antibodies in sow milk is unsatisfactory. Preliminary studies have shown limited success. Emerging evidence suggests that general immune factors in sow milk provide protection to neonatal piglets, particularly, lactoferrin, play a crucial role in protecting piglets by inhibiting PEDV replication. However, the precise mechanism by which lactoferrin exerts its antiviral effects remains unclear. This study sought to clarify these mechanisms through both in vitro and in vivo approaches, proposing that higher concentrations of lactoferrin lead to greater antiviral activity. It was hypothesized that lactoferrin can impede PEDV by blocking its binding to heparan sulfate proteoglycans (HSPG) on the surface of target cells, and molecular docking experiments was conducted to identify the binding sites between lactoferrin and HSPG. Additionally, the findings indicated that lactoferrin can effectively trigger the maturation of porcine dendritic cells and boosts their antigen-presenting functions, thereby improving intestinal mucosal immunity in neonatal piglets against PEDV. Overall, these findings aid to elucidate the antiviral actions and mechanisms of lactoferrin in sow colostrum, offering new insights for the effective prevention and control of PED in neonatal piglets.","PeriodicalId":23658,"journal":{"name":"Veterinary Research","volume":"56 1","pages":"25"},"PeriodicalIF":3.7,"publicationDate":"2025-01-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11786531/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143075795","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Biofilm characterisation of Mycoplasma bovis co-cultured with Trueperella pyogenes. 牛支原体与化脓性真芽孢杆菌共培养的生物膜特性。

IF 3.7 1区农林科学

Veterinary Research Pub Date : 2025-01-30 DOI: 10.1186/s13567-025-01468-1

Koji Nishi, Satoshi Gondaira, Yuki Hirano, Masahide Ohashi, Ayano Sato, Kazuya Matsuda, Tomohito Iwasaki, Takuya Kanda, Ryoko Uemura, Hidetoshi Higuchi

{"title":"Biofilm characterisation of Mycoplasma bovis co-cultured with Trueperella pyogenes.","authors":"Koji Nishi, Satoshi Gondaira, Yuki Hirano, Masahide Ohashi, Ayano Sato, Kazuya Matsuda, Tomohito Iwasaki, Takuya Kanda, Ryoko Uemura, Hidetoshi Higuchi","doi":"10.1186/s13567-025-01468-1","DOIUrl":"10.1186/s13567-025-01468-1","url":null,"abstract":"Mycoplasma pneumonia, caused by Mycoplasma bovis (Mycoplasmopsis bovis; M. bovis), is linked with severe inflammatory reactions in the lungs and can be challenging to treat with antibiotics. Biofilms play a significant role in bacterial persistence and contribute to the development of chronic lesions. A recent study has shown that polymicrobial interactions between species are an important factor in biofilm formation, yet the precise mechanism of biofilm formation in M. bovis remains unknown. By assuming multiple pathogen infections in the bovine respiratory disease complex (BRDC), this study examined the characterisation of the polymicrobial relationship between M. bovis and Trueperella pyogenes (T. pyogenes) during biofilm formation. Autopsies were performed on four Holstein calves (two chronic Mycoplasma pneumonia calves and two control calves). Bacterium-like aggregation structures (> 10 μm), which were assumed to be biofilms of M. bovis in vivo, were observed adhering to the cilia in calves with Mycoplasma pneumonia. M. bovis released an extracellular matrix to connect with neighbouring bacteria and form a mature biofilm on the plate. Biofilm formation in the co-culture of M. bovis and T. pyogenes (strain T1: 1 × 105 and 1 × 106 CFU/well) significantly increased (p < 0.05 and p < 0.01; 64.1% and 64.8% increase) compared to that in a single culture of these bacteria. Furthermore, some large aggregates (> 40 μm), composed of M. bovis and T. pyogenes, were observed. The morphological characteristics of this biofilm were similar to those observed in vivo compared to a single culture. In conclusion, the polymicrobial interaction between M. bovis and T. pyogenes induces biofilm formation, which is associated with increased resistance to antimicrobial agents, and this exacerbates the progression of chronic Mycoplasma pneumonia.","PeriodicalId":23658,"journal":{"name":"Veterinary Research","volume":"56 1","pages":"22"},"PeriodicalIF":3.7,"publicationDate":"2025-01-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11783866/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143068115","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

IFN-β production induced by PRRSV is affected by GP3 quantity control and CLND4 interaction. PRRSV诱导IFN-β的产生受GP3数量控制和CLND4相互作用的影响。

IF 3.7 1区农林科学

Veterinary Research Pub Date : 2025-01-28 DOI: 10.1186/s13567-025-01455-6

Dexin Li, Xinyu Cui, Yingchao Li, Qin Zhang, Hongyan Gao, Youbo Li, Yanmeng Hou, Hongjie Yuan, Yihong Xiao

{"title":"IFN-β production induced by PRRSV is affected by GP3 quantity control and CLND4 interaction.","authors":"Dexin Li, Xinyu Cui, Yingchao Li, Qin Zhang, Hongyan Gao, Youbo Li, Yanmeng Hou, Hongjie Yuan, Yihong Xiao","doi":"10.1186/s13567-025-01455-6","DOIUrl":"10.1186/s13567-025-01455-6","url":null,"abstract":"Porcine reproductive and respiratory syndrome virus (PRRSV) is one of the most harmful pathogens in the swine industry. Our previous studies demonstrated that the small extracellular domain (ECL2) of CLDN4 effectively blocks PRRSV infection. In this study, we explored the in vivo administration of swine ECL2 (sECL2) and found that it blocked HP-PRRSV infection and alleviated histopathological changes in organs. Notably, sECL2 stimulated cytokine production in the lungs. We observed that CLDN4 upregulated the expression of IFN-β at low doses of GP3. While high doses of GP3 inhibited the activity of the IFN-β promotor, regardless of whether CLDN4 was present. GP3 also downregulated IFN-β by decreasing the phosphorylation of TBK1 and IRF3. These findings highlight functional differences in GP3 under quantity control, which account for the variations in IFN-β induction during the early and late stages of infection. Our results indicate that sECL2 is a promising candidate drug for developing treatments to control PRRS.","PeriodicalId":23658,"journal":{"name":"Veterinary Research","volume":"56 1","pages":"21"},"PeriodicalIF":3.7,"publicationDate":"2025-01-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11776237/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143060850","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Binding and cleavage of pro-urokinase by a tegument extract of Fasciola hepatica newly excysted juveniles activate the host fibrinolytic system. 肝片形吸虫幼虫被皮提取物结合和裂解尿激酶原体，可激活宿主纤维蛋白溶解系统。

IF 3.7 1区农林科学

Veterinary Research Pub Date : 2025-01-25 DOI: 10.1186/s13567-025-01449-4

Judit Serrat, María Torres-Valle, Carolina De Marco Verissimo, Mar Siles-Lucas, Javier González-Miguel

{"title":"Binding and cleavage of pro-urokinase by a tegument extract of Fasciola hepatica newly excysted juveniles activate the host fibrinolytic system.","authors":"Judit Serrat, María Torres-Valle, Carolina De Marco Verissimo, Mar Siles-Lucas, Javier González-Miguel","doi":"10.1186/s13567-025-01449-4","DOIUrl":"10.1186/s13567-025-01449-4","url":null,"abstract":"Plasmin, the final product of fibrinolysis, is a broad-spectrum serine protease that degrades extracellular matrix (ECM) components, a function exploited by multiple pathogens for dissemination purposes. The trematode Fasciola hepatica is the leading cause of fasciolosis, a major disease of livestock and an emerging zoonosis in humans. Infection success depends on the ability of F. hepatica newly excysted juveniles (FhNEJ) to penetrate the host intestinal wall, a process that remains incompletely understood. We have previously shown that FhNEJ are capable of binding plasminogen (PLG), the zymogen of plasmin, on their tegument surface, which leads to plasmin generation in the presence of host-derived PLG activators and subsequent degradation of laminin, a major component of the intestinal ECM. Here, we describe the interaction between a tegument extract of FhNEJ and the precursor of the urokinase-type PLG activator (pro-u-PA). We found that F. hepatica cathepsins B3, L3, enolase and glutathione S-transferase mediate this interaction, suggesting a multifactorial or moonlighting role for these proteins. Additionally, our results revealed that the tegument of FhNEJ contains a protease that is capable of cleaving and activating pro-u-PA into its catalytically active form, which positively impacts the capacity of the parasites to generate plasmin from the host PLG. Collectively, our findings indicate that FhNEJ interact with the host fibrinolytic system at multiple levels, reinforcing the potential of targeting this interaction as a strategy to prevent FhNEJ trans-intestinal migration and infection success.","PeriodicalId":23658,"journal":{"name":"Veterinary Research","volume":"56 1","pages":"20"},"PeriodicalIF":3.7,"publicationDate":"2025-01-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11762853/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143042362","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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