Veterinary Research最新文献_第9页

Development of a novel chimeric lysin to combine parental phage lysin and cefquinome for preventing sow endometritis after artificial insemination. 结合亲代噬菌体溶酶和头孢醌的新型嵌合溶酶的研制预防母猪人工授精后子宫内膜炎。

IF 3.7 1区农林科学

Veterinary Research Pub Date : 2025-02-11 DOI: 10.1186/s13567-025-01457-4

Xin-Xin Li, Zi-Qiang Hong, Zhi-Xuan Xiong, Li-Wen Zhang, Shuang Wang, Pan Tao, Pin Chen, Xiang-Min Li, Ping Qian

{"title":"Development of a novel chimeric lysin to combine parental phage lysin and cefquinome for preventing sow endometritis after artificial insemination.","authors":"Xin-Xin Li, Zi-Qiang Hong, Zhi-Xuan Xiong, Li-Wen Zhang, Shuang Wang, Pan Tao, Pin Chen, Xiang-Min Li, Ping Qian","doi":"10.1186/s13567-025-01457-4","DOIUrl":"10.1186/s13567-025-01457-4","url":null,"abstract":"Sow endometritis is usually caused by multiple species of pathogenic bacteria. Numerous isolates from endometritis patients have developed antimicrobial resistance. Thus, novel antibacterial agents and strategies to combat endometritis are needed. A total of 526 bacteria, including Staphylococcus spp. (26.3%), Streptococcus spp. (12.3%), E. coli (28.9%), Enterococcus spp. (20.1%), Proteus spp. (9.5%), and Corynebacterium spp. (2.8%), were isolated from sows with endometritis. We constructed a novel chimeric lysin, ClyL, which is composed of a cysteine- and histidine-dependent amidohydrolase/peptidase (CHAP) catalytic domain from the phage lysin LysGH15 and a cell wall-binding domain (CBD) from the prophage lysin Lys0859. The activities of ClyL and Lys0859 were most pronounced for the Staphylococcus and Streptococcus strains isolated from sow endometritis and bovine mastitis, respectively. ClyL and Lys0859 were combined to create a phage lysin cocktail, which demonstrated a synergistic effect against the coinfection of Staphylococcus and Streptococcus in vitro and in vivo. Furthermore, the combination of phage lysin cocktail and cefquinome had a synergistic bactericidal effect on boar semen that did not influence the activity of sperm. Remarkably, the incidence rate of sow endometritis was 0% (0/7) when the combination of phage lysin cocktail and cefquinome was used in semen via artificial insemination compared with 50% (3/6) when PBS was administered. Overall, the administration of a phage lysin cocktail and cefquinome in semen via artificial insemination is a promising novel strategy to prevent sow endometritis after artificial insemination.","PeriodicalId":23658,"journal":{"name":"Veterinary Research","volume":"56 1","pages":"39"},"PeriodicalIF":3.7,"publicationDate":"2025-02-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11816537/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143400337","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Opportunistic pathogens are prevalent across the culturable exogenous and endogenous microbiota of stable flies captured at a dairy facility. 机会致病菌普遍存在于奶牛设施捕获的稳定苍蝇的可培养外源性和内源性微生物群中。

IF 3.7 1区农林科学

Veterinary Research Pub Date : 2025-02-11 DOI: 10.1186/s13567-025-01458-3

Andrew J Sommer, Courtney L Deblois, Andrew D J Tu, Garret Suen, Kerri L Coon

{"title":"Opportunistic pathogens are prevalent across the culturable exogenous and endogenous microbiota of stable flies captured at a dairy facility.","authors":"Andrew J Sommer, Courtney L Deblois, Andrew D J Tu, Garret Suen, Kerri L Coon","doi":"10.1186/s13567-025-01458-3","DOIUrl":"10.1186/s13567-025-01458-3","url":null,"abstract":"Stable flies in the genus Stomoxys are highly abundant, blood-feeding pests on dairy farms; however, their role in the carriage and potential transmission of pathogens is largely understudied. Here, we report on the frequency and distribution of culturable bacteria collected from Stomoxys flies captured in free stall barns and nearby calf hutches over a three-month period on a focal research farm in Wisconsin, USA. Mastitis-associated bacterial taxa, including Staphylococcus, Escherichia, Enterobacter, and Klebsiella spp., were frequently isolated from pooled samples of the internal or external portions of the flies. Conversely, selective enrichment protocols from these samples yielded only a single isolate of Salmonella and no enterohemorrhagic Escherichia coli O157. Neither trap location nor time of capture had a significant impact on the observed frequency of most bacterial genera isolated from the flies. Our results confirm that Stomoxys flies harbor both mastitis-associated bacterial taxa and bacterial taxa associated with opportunistic infections in humans. Further research into the transmission of fly-associated microbes could be important in the control of mastitis or other bacterial diseases on dairy farms.","PeriodicalId":23658,"journal":{"name":"Veterinary Research","volume":"56 1","pages":"40"},"PeriodicalIF":3.7,"publicationDate":"2025-02-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11817017/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143400338","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genetic and molecular characterization of a novel reassortant H3N2 influenza virus from a sick pig in Eastern China in 2019. 2019年中国东部一株新型病猪重组H3N2流感病毒的遗传和分子特征

IF 3.7 1区农林科学

Veterinary Research Pub Date : 2025-02-10 DOI: 10.1186/s13567-025-01462-7

Fan Yang, Linfang Cheng, Fumin Liu, Hangping Yao, Nanping Wu, Lihua Xu, Haibo Wu

{"title":"Genetic and molecular characterization of a novel reassortant H3N2 influenza virus from a sick pig in Eastern China in 2019.","authors":"Fan Yang, Linfang Cheng, Fumin Liu, Hangping Yao, Nanping Wu, Lihua Xu, Haibo Wu","doi":"10.1186/s13567-025-01462-7","DOIUrl":"10.1186/s13567-025-01462-7","url":null,"abstract":"Swine influenza viruses (SIVs) cause clinical respiratory symptoms associated with high mortality rates among pigs. Because pigs can be a \"mixing vessel\" for influenza viruses, the SIV might pose a serious threat to animal and human health. In this study, an H3N2 SIV [A/swine/Zhejiang/19/2019(H3N2) (ZJ-SW19)] was isolated from a sick pig in Eastern China in 2019, and its molecular genetics were characterized. Phylogenetic analysis demonstrated the hemagglutinin (HA) and neuraminidase (NA) segments of ZJ-SW19 are highly homologous with those of H3N2 SIVs, belonging to human-like lineages; in contrast, the remaining six SIV segments (PB2, PB1, PA, NP, M, and NS) demonstrate the highest similarity with H1N1 SIVs isolated in East Asia during 2014-2020. The in vitro analysis of the virus's growth kinetics revealed that ZJ-SW19 can replicate efficiently in various mammalian and avian cell lines (including MDCK, A549, and DF-1). The receptor-binding analysis results indicated that ZJ-SW19 can bind to human-like receptors (α-2,6-linked sialic acid) and avian-like receptors (α-2,3-linked sialic acid). Moreover, ZJ-SW19 demonstrated significant differences compared with avian- and human-origin H3N2 influenza viruses in the antigenic analysis. Finally, in the pathogenicity test, ZJ-SW19 effectively replicated in the mouse lungs with moderate virulence. Therefore, continuous circulation of novel reassortant H3N2 SIVs indicates the need for long-term, close surveillance of influenza viruses in pig herds.","PeriodicalId":23658,"journal":{"name":"Veterinary Research","volume":"56 1","pages":"38"},"PeriodicalIF":3.7,"publicationDate":"2025-02-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11808988/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143392012","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Limited transmission of avian influenza viruses, avulaviruses, coronaviruses and Chlamydia sp. at the interface between wild birds and a free-range duck farm. 禽流感病毒、牛瘟病毒、冠状病毒及衣原体在野鸟与放养鸭场交界处传播有限。

IF 3.7 1区农林科学

Veterinary Research Pub Date : 2025-02-08 DOI: 10.1186/s13567-025-01466-3

Chloé Le Gall-Ladevèze, Benjamin Vollot, Julien Hirschinger, Laëtitia Lèbre, Rachid Aaziz, Karine Laroucau, Jean-Luc Guérin, Mathilde Paul, Julien Cappelle, Guillaume Le Loc'h

{"title":"Limited transmission of avian influenza viruses, avulaviruses, coronaviruses and Chlamydia sp. at the interface between wild birds and a free-range duck farm.","authors":"Chloé Le Gall-Ladevèze, Benjamin Vollot, Julien Hirschinger, Laëtitia Lèbre, Rachid Aaziz, Karine Laroucau, Jean-Luc Guérin, Mathilde Paul, Julien Cappelle, Guillaume Le Loc'h","doi":"10.1186/s13567-025-01466-3","DOIUrl":"10.1186/s13567-025-01466-3","url":null,"abstract":"Recent outbreaks of highly pathogenic avian influenza in Europe have raised questions regarding the epidemiological role of commensal wild birds on free-range poultry farms. This study aimed to assess the prevalence of avian influenza viruses (AIV), avulaviruses, coronaviruses and Chlamydia sp. in commensal wild birds on a free-range duck farm in southwestern France and to evaluate possible transmission events at the wild‒domestic interface. From 2019 through 2021, a longitudinal study was conducted on wild birds, domestic ducks and their shared environment on farms. Commensal wild birds were captured and sampled for blood and swabs, and fresh feces from cattle egrets visiting the farm were collected. In parallel, domestic ducks were sampled, and environmental samples were collected. The presence of the four pathogens was tested by q(RT-)PCR, and the immunity of wild birds to AIV and Newcastle disease virus (NDV) was tested by ELISA. Wild birds were found to shed AIV and Chlamydia only, with a low prevalence (< 3%). The seroprevalence rates were less than 10% for AIV and less than 4.5% for NDV. No significant temporal trend was identified. Ducks and their environment frequently test simultaneously positive for the same pathogens (19 to 44% of flocks), mostly during fall‒winter. In addition to unrelated temporal patterns, the identification of pathogens in wild birds seemed unrelated to that in domestic ducks. These results suggest a low transmissibility of the avian pathogens tested in our study at the wild‒domestic interface and highlight the limited contribution of commensal wild birds in comparison with free-range poultry to the global microbiological pressure on the environment.","PeriodicalId":23658,"journal":{"name":"Veterinary Research","volume":"56 1","pages":"36"},"PeriodicalIF":3.7,"publicationDate":"2025-02-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11806813/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143374589","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Engineered Bacillus subtilis WB600/ZD prevents Salmonella Infantis-induced intestinal inflammation and alters the colon microbiota in a mouse model. 在小鼠模型中，工程枯草芽孢杆菌WB600/ZD可预防婴儿沙门氏菌引起的肠道炎症并改变结肠微生物群。

IF 3.7 1区农林科学

Veterinary Research Pub Date : 2025-02-08 DOI: 10.1186/s13567-024-01438-z

Wei Li, Xue Wang, Keyuan Chen, Yaohong Zhu, Guiyan Yang, Yipeng Jin, Jiufeng Wang

{"title":"Engineered Bacillus subtilis WB600/ZD prevents Salmonella Infantis-induced intestinal inflammation and alters the colon microbiota in a mouse model.","authors":"Wei Li, Xue Wang, Keyuan Chen, Yaohong Zhu, Guiyan Yang, Yipeng Jin, Jiufeng Wang","doi":"10.1186/s13567-024-01438-z","DOIUrl":"10.1186/s13567-024-01438-z","url":null,"abstract":"Antimicrobial peptides (AMPs) are instrumental in maintaining intestinal homeostasis and have emerged as potential therapeutic candidates for ameliorating intestinal bacterial infections. However, the intrinsic instability associated with the in vivo delivery of AMPs constitutes a substantial impediment to their therapeutic efficacy in treating infections. In this study, we genetically modified Bacillus subtilis (B. subtilis) WB600 to express Zophobas atratus defensin (ZD), an antimicrobial peptide with broad-spectrum activity isolated from Zophobas atratus, for oral administration. This engineered strain effectively protects against Salmonella Infantis (S. Infantis) infection in mice. Pretreatment with WB600/ZD prevented NF-κB pathway activation induced by S. Infantis infection and increased expression of antioxidant and tight junction proteins, thus alleviating the severity of intestinal inflammation in both the jejunum and ileum (P < 0.01). Moreover, WB600/ZD pretreatment facilitated the growth of beneficial bacteria such as Lachnospiraceae, Butyricicoccus, Eubacterium_xylanophilum, and Clostridia_UCG-014 while decreasing the abundance of pathogenic bacteria such as Escherichia-Shigella and Salmonella (P < 0.05). In conclusion, this study underscores the protective effects of WB600/ZD on S. Infantis-induced intestinal inflammation, suggesting that oral delivery of B. subtilis WB600/ZD may be a promising prophylactic strategy for combating bacterial infections in the intestine.","PeriodicalId":23658,"journal":{"name":"Veterinary Research","volume":"56 1","pages":"35"},"PeriodicalIF":3.7,"publicationDate":"2025-02-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11806837/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143371118","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A novel high-throughput screen identifies phenazine-1-carboxylic acid as an inhibitor of African swine fever virus replication in primary porcine alveolar macrophages. 一种新的高通量筛选鉴定了吩那嗪-1-羧酸作为非洲猪瘟病毒在原代猪肺泡巨噬细胞复制的抑制剂。

IF 3.7 1区农林科学

Veterinary Research Pub Date : 2025-02-08 DOI: 10.1186/s13567-025-01467-2

Jing Lan, Rui Luo, Di Liu, Changxing Qi, Xin Song, Zhanhao Lu, Ruojia Huang, Yuying Yang, Yuan Sun, Yonghui Zhang, Tao Wang, Hua-Ji Qiu

{"title":"A novel high-throughput screen identifies phenazine-1-carboxylic acid as an inhibitor of African swine fever virus replication in primary porcine alveolar macrophages.","authors":"Jing Lan, Rui Luo, Di Liu, Changxing Qi, Xin Song, Zhanhao Lu, Ruojia Huang, Yuying Yang, Yuan Sun, Yonghui Zhang, Tao Wang, Hua-Ji Qiu","doi":"10.1186/s13567-025-01467-2","DOIUrl":"10.1186/s13567-025-01467-2","url":null,"abstract":"African swine fever (ASF), caused by African swine fever virus (ASFV), has resulted in significant economic impacts on the global swine industry. Currently, there is no safe and effective commercial vaccine available for ASFV. Thus, the development of effective and readily available therapeutics for ASF is urgently needed. To conduct high-throughput screening (HTS) for anti-ASFV drugs, we initially developed a recombinant dual-reporter virus (rASFV-Gluc/EGFP) using the virulent strain ASFV HLJ/18 (ASFV-WT). The enhanced green fluorescent protein (EGFP)- and Gaussia luciferase (Gluc)-encoding genes were incorporated downstream of the ASFV MGF300-4L gene without disrupting viral genes. The growth kinetics, hemadsorption, and transmission electron microscopy analysis of rASFV-Gluc/EGFP in primary porcine alveolar macrophages (PAMs) revealed that rASFV-Gluc/EGFP exhibits similar biological characteristics to ASFV-WT. Furthermore, analysis of Gluc activities, fluorescence, and next-generation sequencing indicated that rASFV-Gluc/EGFP maintains good genetic stability after 20 consecutive passages in PAMs. Using the HTS platform established with rASFV-Gluc/EGFP, we screened and identified phenazine-1-carboxylic acid (PCA) as an effective inhibitor of ASFV replication from 246 small molecule compounds in PAMs. Importantly, PCA was found to reduce ASFV replication by as much as 100-fold at a concentration of 25 μM. Overall, this study suggests that rASFV-Gluc/EGFP is suitable for rapid screening of anti-ASFV drugs. Importantly, we showed that PCA has significant anti-ASFV activity in PAMs.","PeriodicalId":23658,"journal":{"name":"Veterinary Research","volume":"56 1","pages":"37"},"PeriodicalIF":3.7,"publicationDate":"2025-02-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11806816/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143374577","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The unique activity of the bone morphogenetic protein TGH4 affects the embryonic development of Trichinella spiralis and the establishment of vaccine protection. 骨形态发生蛋白TGH4的独特活性影响旋毛虫的胚胎发育和疫苗保护的建立。

IF 3.7 1区农林科学

Veterinary Research Pub Date : 2025-02-07 DOI: 10.1186/s13567-025-01473-4

Wenjie Shi, Yi Liu, Yan Liu, Xue Bai, Yue Liang, Yaming Yang, Fangwei Wu, Mingyuan Liu, Ning Xu

{"title":"The unique activity of the bone morphogenetic protein TGH4 affects the embryonic development of Trichinella spiralis and the establishment of vaccine protection.","authors":"Wenjie Shi, Yi Liu, Yan Liu, Xue Bai, Yue Liang, Yaming Yang, Fangwei Wu, Mingyuan Liu, Ning Xu","doi":"10.1186/s13567-025-01473-4","DOIUrl":"10.1186/s13567-025-01473-4","url":null,"abstract":"In recent years, animal outbreaks of trichinellosis have been reported in Mexico, China, Algeria, Croatia and others, which is a challenge to meat safety and public health. Vaccination is an important means to control trichinellosis, but one of the main challenges in vaccine development is screening for protective candidate antigens. Bone morphogenetic proteins (BMP)), which are potential vaccine research targets, play key roles in the growth and development of metazoans. The BMP homologue TGH4 was identified from Trichinella spiralis (T. spiralis), and recombinant C-terminal mature rTGH4-m had BMP activity and exerted regulatory effects on both mouse fibroblast and T. spiralis embryonic development. The construction of a protein mutant without activation potential confirmed that BMP activity had a negative regulatory effect on the establishment of immune protection by affecting the innate, adaptive, and humoral immunity of mice. Stripping protein activity can enhance immune protection and host resistance to T. spiralis. Our findings demonstrate that the TGH4 mutant is an important vaccine candidate antigen that blocks embryonic development, kills larvae, and provides insight into parasite vaccine research.","PeriodicalId":23658,"journal":{"name":"Veterinary Research","volume":"56 1","pages":"31"},"PeriodicalIF":3.7,"publicationDate":"2025-02-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11803935/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143366111","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Correction: Genomic analysis and replication kinetics of the closely related EHV-1 neuropathogenic 21P40 and abortigenic 97P70 strains. 更正：密切相关的EHV-1神经致病性21P40和流产性97P70菌株的基因组分析和复制动力学。

IF 3.7 1区农林科学

Veterinary Research Pub Date : 2025-02-07 DOI: 10.1186/s13567-025-01464-5

Eslam Mohamed, Ines Zarak, Nick Vereecke, Sebastiaan Theuns, Kathlyn Laval, Hans Nauwynck

引用次数: 0

The tryptophan-aspartate (WD) repeat domain of bovine Coronin-1A promotes mycobacterial survival by inhibiting calcium signaling-mediated phagosome-lysosome fusion. 牛冠状蛋白1a的色氨酸-天冬氨酸（WD）重复结构域通过抑制钙信号介导的吞噬体-溶酶体融合促进分枝杆菌的存活。

IF 3.7 1区农林科学

Veterinary Research Pub Date : 2025-02-07 DOI: 10.1186/s13567-025-01471-6

Jing Yang, Zhunan Li, Aicong Li, Yayi Liu, Xinyan Zhang, Yong Zhang, Yuanpeng Gao

{"title":"The tryptophan-aspartate (WD) repeat domain of bovine Coronin-1A promotes mycobacterial survival by inhibiting calcium signaling-mediated phagosome-lysosome fusion.","authors":"Jing Yang, Zhunan Li, Aicong Li, Yayi Liu, Xinyan Zhang, Yong Zhang, Yuanpeng Gao","doi":"10.1186/s13567-025-01471-6","DOIUrl":"10.1186/s13567-025-01471-6","url":null,"abstract":"Bovine tuberculosis is a chronic consumptive zoonosis, causing significant economic losses and critical public health risks. Coronin-1A is a host cytoskeleton-associated protein that is crucial for understanding the inhibition of phagosome-lysosome fusion by Mycobacterium tuberculosis (M.tb) to evade host innate immune clearance. However, the involvement of bovine Coronin-1A (bCoronin-1A) in M.tb infection and whether it can be manipulated so as to enhance host resistance against bovine tuberculosis remains to be seen. Here, we explored the role of bCoronin-1A in phagosome-lysosome fusion in M.tb-infected macrophages. We found that bCoronin-1A was upregulated at both the transcriptional and protein levels following M.tb infection of embryonic bovine lung (EBL) cells. Notably, bCoronin-1A was recruited to M.tb-containing phagosomes where it hindered phagosome-lysosome fusion, leading to increased intracellular mycobacterial survival. Further investigation revealed that mycobacterial lipoamide dehydrogenase C (LpdC) interacted with a single tryptophan-aspartate (WD) unit within the WD repeat domain of bCoronin-1A to sequester it on the phagosomes. The WD repeat domain mediated a decrease in intracellular calcium levels, which reduced levels of calmodulin-dependent kinase II (CaMKII) and its activated forms, thereby inhibiting lysosomal delivery. Overall, our findings revealed that bCoronin-1A had a critical impact on mycobacterial survival in macrophages by inhibiting calcium-mediated phagosome-lysosome fusion. This suggests that targeting bCoronin-1A as a key factor influencing mycobacterial survival may be an effective breeding strategy to develop tuberculosis-resistant dairy cows.","PeriodicalId":23658,"journal":{"name":"Veterinary Research","volume":"56 1","pages":"33"},"PeriodicalIF":3.7,"publicationDate":"2025-02-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11806767/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143371140","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Emergence of a novel porcine pestivirus with potential for cross-species transmission in China, 2023. 2023年中国出现一种具有跨物种传播潜力的新型猪瘟病毒。

IF 3.7 1区农林科学

Veterinary Research Pub Date : 2025-02-07 DOI: 10.1186/s13567-025-01472-5

Li-Shuang Deng, Tong Xu, Zhi-Wen Xu, Ling Zhu

{"title":"Emergence of a novel porcine pestivirus with potential for cross-species transmission in China, 2023.","authors":"Li-Shuang Deng, Tong Xu, Zhi-Wen Xu, Ling Zhu","doi":"10.1186/s13567-025-01472-5","DOIUrl":"10.1186/s13567-025-01472-5","url":null,"abstract":"Pestiviruses, RNA viruses belonging to the Flaviviridae family, have a broad host range. Their pathogenicity varies greatly and they have caused significant economic losses in animal husbandry. In this study, a novel pestivirus, porcine abortion-associated pestivirus (PAAPeV), was isolated from pigs in China in 2023. Clinically, PAAPeV causes abortions in sows and leads to congenital tremors and death in piglets. PAAPeV replicates efficiently in ST cells. Morphologically, PAAPeV virions are spherical particles with a diameter of approximately 80 nm. Phylogenetic analysis reveals that PAAPeV is closely related to Wenzhou Pipistrellus abramus pestivirus and clusters into a distinct branch, suggesting that it represents a new species: Pestivirus chinensis. Animal experiments demonstrated that PAAPeV-infected piglets and mice exhibit significant histopathological changes. In piglets, histopathological examination revealed myocarditis, hepatitis, glial vacuolation and cerebrovascular inflammation. In mice, findings included hepatic monocyte aggregation, glomerular atrophy, pulmonary edema, inflammatory cell infiltration, and capillary dilation. Viremia has been detected in both piglets and mice, with high viral genome loads found in various organs. In vitro results revealed that PAAPeV replicates in ST cells and, to a lesser extent, in human (A549 and HepG2) and monkey (Vero) cells. Overall, PAAPeV infects both pigs and mice and has the potential to infect other mammals, indicating its ability for cross-species transmission. This poses significant risks to the pig industry and public health. Strengthening monitoring and prevention efforts for PAAPeV is crucial. Our findings greatly increase the understanding of pestivirus diversity and its broader pathogen spectrum.","PeriodicalId":23658,"journal":{"name":"Veterinary Research","volume":"56 1","pages":"32"},"PeriodicalIF":3.7,"publicationDate":"2025-02-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11804013/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143366105","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0